JPH01111656A - Container for standard liquid etc. - Google Patents

Abstract

PURPOSE:To prevent the evaporation of a solvent and the use of the liquid concentrated to an undesirable extent, by providing a container with the members floating on the liquid surface and occupying at least one-half of the liquid surface and with the spherical members of not less than four having a greater specific gravity than that of the liquid and placed therein to a predeter mined height from the inner surface of the container bottom. CONSTITUTION:It is desirable that a clearance between a floating member and the inner surface of wall of a container in the liquid storage portion be made as small as possible. And it is important that the material of the floating member do not affect the liquid composition. By forming a hole in the middle of this floating member, the liquid can be removed by a pipette or the like through the hole, without forcing the floating member into the liquid. The material of the spherical member is greater in specific gravity than the liquid and it is important that this material be the type which does not affect the liquid composition. These spherical members are placed in the container to a depth corresponding to that of the liquid in the minimum limit of the amount, with changes in the liquid concentration resulting from evaporation confined within a practically negligible limit.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a container for storing a solution having a fixed composition and dispensing the same a plurality of times for use.

Specifically, it is a container for containing solutions that serve as analysis standards such as reference solutions, standard solutions, calibrators, and control solutions, which are frequently used in the field of analytical chemistry, particularly in the field of clinical chemistry testing.

[Prior art] In the field of analytical chemistry, especially in the field of clinical chemistry testing, analysis is often performed using a solution of a certain concentration as a standard.The standard solution includes a standard solution, a calibration solution, and a calibrator-1 test solution. There are various liquids called , control liquid, reference liquid, etc. In particular, in the case of commercially available products that are widely used on a daily basis in the field of clinical chemistry testing and are often commercially available, these solutions are generally housed in 5 to 10 vials. Because the area of the opening is large compared to the volume, repeated opening and closing of the lid to take out the liquid causes evaporation of the solvent, which has a non-negligible effect on the accuracy of analysis. In principle, containers for standard solutions, etc. should be tightly capped, and the cap should be removed only when dispensing with a pipette, and then capped again after aliquoting. During the time it is taken out and used, evaporation progresses little by little, and as the amount of remaining liquid decreases, its concentration increases.
There are times when it is unavoidable to continue working without the stopper, and in this case evaporation proceeds rapidly, resulting in a higher concentration of the solution used as the standard for analysis, making it impossible to obtain accurate measurement results.

Biological body fluids (whole blood, plasma,
Quantitative analysis of target components (analytes) in cerebrospinal fluid, urine, saliva, etc.) requires high precision, but the concentration of the solution used as a standard in this case can cause measurement errors, making analytical measurements difficult. It may become a serious problem. Especially Na in the blood,
The concentration of electrolyte ions such as Ni' and Cl- is maintained at homeostasis within a very narrow range in a normal living body, and measurement errors are extremely important in clinical diagnosis. Against this background, there has been a need for an easy-to-use, inexpensive container that can suppress concentration caused by evaporation of the solvent.

In order to reduce concentration changes in ordinary bottles, it is possible to reduce the inner diameter of the liquid storage part of the bottle or to make the diameter of the bottle mouth sufficiently small. However, there is a limit to reducing the inner diameter of the bottle when considering the stability of the container against overturning.

Furthermore, reducing the diameter of the stopper has its limits in terms of ease of use.

Additionally, the use of liquids that have become undesirably concentrated due to evaporation of the solvent must be avoided; measuring the concentration of stored liquids each time they are used is time-consuming and impractical. .

[Technical Problems to be Solved] In the present invention, in a liquid container having an opening at the top that can be sealed tightly, a wide-mouth bottle of the shape conventionally used is used, and the liquid can be taken out without inconvenience. , it is a technical problem to prevent evaporation of the solvent and to prevent the use of undesirably concentrated liquids.

[Means for solving technical problems] In the present invention, a liquid container is provided with an opening at the top that can be sealed tightly, is able to float on the liquid surface, has a hole communicating with the liquid surface at or near the center, and has a hole above the liquid surface. By providing a member in the container that covers at least one-half of the container, and filling four or more balls made of a material that does not affect the properties of the liquid and having a specific gravity larger than the liquid from the inner bottom surface of the container to a certain height. , the above problem was solved.

[Details of specific configuration] The member floating on the liquid surface is usually plate-shaped, but may also be cone-shaped. It is preferable that the gap between the opening and the opening be as small as possible.If the contour is similar to the shape of the inside of the container, it is advantageous to reduce the gap between the opening and the inner wall of the opening. Where the container is most commonly cylindrical, the member is preferably disc-shaped, or optionally conical.

If it is made of a material with a specific gravity greater than that of the liquid, it must be hollow so that the bulk specific gravity is less than the specific gravity of the liquid.

If it is solid (having no cavities), it must be made of a material whose specific gravity is lower than that of the liquid.

It is important that the material of the member floated on the liquid surface does not affect the liquid composition (1nnert). Examples of suitable materials include glass, silicone rubber, Teflon, polyvinyl chloride, polystyrene, acrylic resin, etc. with a specific gravity of more than 1, and polyethylene and polypropylene with a specific gravity of less than 1. It goes without saying that the container must be able to be sealed tightly, and even if the floating member made of flexible material is larger than the lid, it can be bent and inserted into the container through the mouth of the container. Even if the whole body is not flexible, if it can be bent along a straight line passing through the center, the same purpose can be achieved.

By providing a hole in the center of the floating member, the liquid can be taken out with a pipette or the like without pushing the member down. The size of the hole must therefore be large enough to allow the tip of a pipette or the like to be inserted therein. For example, if the hole is circular, it is preferably about 2 to 10 mm in diameter.

The spheres to be filled in the container of the present invention do not have to be perfect spheres, and may have, for example, flat portions, depressions, or small holes through them; however, it is desirable that the surface of the spheres be easy to clean. It is preferable that the size of the container be such that it can be inserted into the container through the mouth of the container, but even if it is larger than the mouth of the container, it can be placed in advance when manufacturing the container.

It is preferable that the device for collecting liquid, such as a pipette, be of a size that cannot be entered from the tip;
A diameter of about m11 to 10III11 is preferable.

If the inner diameter of the tip of the pipette is relatively thin, it is 0.5 am.
It would be fine to a certain extent.

The material constituting the sphere must have a specific gravity greater than that of the liquid. It is important that the material of the sphere has no influence on the liquid composition. Examples of suitable materials include glass, polyvinyl chloride, polystyrene, acrylic resin, tetrafluoroethylene resin, silicone rubber, nickel, and stainless steel. 2f
Materials of I or 3F1 or higher may be combined (for example, stainless steel and polystyrene, glass and polyvinyl chloride)9 The sphere may be hollow, but the overall specific gravity must be greater than that of the liquid.

The depth at which the sphere is filled is selected so that liquid can be collected freely only in a liquid state where concentration due to evaporation can be practically ignored, that is, it corresponds to the minimum amount of liquid within the range where concentration due to evaporation can be practically ignored. Fill the sphere to the desired depth.

[Example 1] A glass bulb with an outer diameter of 5 Ta was placed in a conventionally used glass vial (inner diameter of the opening: inner diameter of the cylindrical body: 23I*III: height: 50mm). Fill it to a depth of about 21mm (from the top of the top sphere to the bottom), and then fill it with polyvinyl chloride pipe (outer diameter 6mm, inner diameter 4mm: length 42mm).
After inserting a donut-shaped ring with an outer diameter of 20 mm with both ends of the parrot hermetically joined, a concentration of 4.2 milligram equivalents/
Reference solution for potassium ion measurement in No. 1 9+*j! was injected. 10ml+ of reference solution for potassium ion measurement was injected. Since the glass bulb is submerged, this container has a liquid level of 4-1
Liquid cannot be collected below.

With the ring floating on the liquid surface, the area of the solution in direct contact with the outside air was about 35%, and the evaporation rate was noticeably slowed down.

In other words, when the bottle was opened once a day and 50μβ was taken out, the concentration changes after 30, 60 and 100 days were as shown in Table 1 (the unit of concentration is milligram R/l).
).

If 50 μl is taken out each time the container is opened, approximately 1
It can be taken out 20 times, and the concentration of the last reference solution taken is 4.3 milligrams! /l, the concentration increase upon opening was within 0.1 milligram equivalent/1 from the initial concentration.

When using a container in which only the filling of spheres is omitted, approximately 20
It can be sampled 0 times, but when the sample is near the end, the concentration increases due to evaporation of the liquid, which is 0.2 milligrams per 1/1 from the initial concentration.
That's all.

For comparison, if the insertion of a floating ring is omitted, 1
When opening the cap twice and taking out 50 μl each.

The concentration changes for 30 days, 60 days, 100 days, and 160 days were as shown in Table 2 (concentration units are milligram equivalents/1).

Comparing Tables 1 and 2, it can be seen that when the container of the present invention is used, the change in the concentration of the solution in the container is much smaller than in the conventional container.

The examples described above are for reference solutions for ion-selective electrodes, but they are not limited to this. This is useful when it is desired to avoid changes in the concentration of the contents of the container and analysis errors caused by this change.

[Brief explanation of the drawing]

FIG. 1 is a sectional view showing a specific example of the container of the present invention. A
and C indicates a case where the floating member 5 has a cavity, and B indicates a case where the floating member 5 has a conical shape without a cavity. FIG. 2 is a sectional view showing a conventional liquid container. The meanings of the symbols in Figures 1 and 2 are as follows. 1: Container body 1a: Male threaded part 2: Lid 2a: Female threaded part 3: Packing 4: Liquid 5: Floating member 6: Sphere Applicant Fuji Photo Film Co., Ltd. Figure 2-3'

Claims (5)

[Claims] (1) A liquid container with a sealable opening at the top, which can float on the liquid surface and which is at least half the liquid surface.
The container has a hole at or near the center that communicates with the liquid surface, and is made of a material that does not affect the properties of the liquid and has a specific gravity larger than that of the liquid. A liquid container characterized in that it is filled with more than one sphere. (2) The container according to claim (1), wherein the liquid is a solution used as a standard in chemical analysis. (3) When the above liquid is chemically analyzed in a clinical chemistry test,
Claim (1) which is a solution used as a standard
Container as described. (4) Claim (1) wherein the cross-sectional shape of the member capable of floating on the liquid surface is similar to the cross-section of the liquid storage portion of the container.
). (5) The container according to claim (1), wherein the member is made of a material having a specific gravity lower than that of the liquid.