JP7850658B2 - ３’オーバーハングを修復する方法 - Google Patents

３’オーバーハングを修復する方法

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Publication number
JP7850658B2
JP7850658B2 JP2022521657A JP2022521657A JP7850658B2 JP 7850658 B2 JP7850658 B2 JP 7850658B2 JP 2022521657 A JP2022521657 A JP 2022521657A JP 2022521657 A JP2022521657 A JP 2022521657A JP 7850658 B2 JP7850658 B2 JP 7850658B2
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JP
Japan
Prior art keywords
dna fragment
fragment
overhang
stranded
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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JP2022521657A
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English (en)
Japanese (ja)
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JP2022553164A (ja
JP2022553164A5 (https=
Inventor
エリーン シャム，
Original Assignee
ガーダント ヘルス， インコーポレイテッド
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Application filed by ガーダント ヘルス， インコーポレイテッド filed Critical ガーダント ヘルス， インコーポレイテッド
Publication of JP2022553164A publication Critical patent/JP2022553164A/ja
Publication of JP2022553164A5 publication Critical patent/JP2022553164A5/ja
Priority to JP2025244666A priority Critical patent/JP2026067857A/ja
Application granted granted Critical
Publication of JP7850658B2 publication Critical patent/JP7850658B2/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2022521657A 2019-10-25 2020-10-23 ３’オーバーハングを修復する方法 Active JP7850658B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025244666A JP2026067857A (ja) 2019-10-25 2025-12-10 ３’オーバーハングを修復する方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962926093P 2019-10-25 2019-10-25
US62/926,093 2019-10-25
PCT/US2020/057189 WO2021081423A1 (en) 2019-10-25 2020-10-23 Methods for 3' overhang repair

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025244666A Division JP2026067857A (ja) 2019-10-25 2025-12-10 ３’オーバーハングを修復する方法

Publications (3)

Publication Number Publication Date
JP2022553164A JP2022553164A (ja) 2022-12-22
JP2022553164A5 JP2022553164A5 (https=) 2023-10-30
JP7850658B2 true JP7850658B2 (ja) 2026-04-23

Family

ID=73452312

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022521657A Active JP7850658B2 (ja) 2019-10-25 2020-10-23 ３’オーバーハングを修復する方法
JP2025244666A Pending JP2026067857A (ja) 2019-10-25 2025-12-10 ３’オーバーハングを修復する方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025244666A Pending JP2026067857A (ja) 2019-10-25 2025-12-10 ３’オーバーハングを修復する方法

Country Status (5)

Country Link
US (2) US11447819B2 (https=)
EP (2) EP4048812B1 (https=)
JP (2) JP7850658B2 (https=)
ES (1) ES2971348T3 (https=)
WO (1) WO2021081423A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024137316A1 (en) 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120172258A1 (en) 2010-12-27 2012-07-05 Ibis Biosciences, Inc. Nucleic acid sample preparation methods and compositions
JP2017537657A (ja) 2014-12-11 2017-12-21 ニユー・イングランド・バイオレイブス・インコーポレイテツド 標的配列の濃縮
WO2018191702A2 (en) 2017-04-14 2018-10-18 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US20030017081A1 (en) 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
US20040209299A1 (en) * 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
US20060078899A1 (en) 2004-10-12 2006-04-13 Scheffer Alicia F Methods and compositions for reducing label variation in array-based comparative genome hybridization assays
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
LT2756098T (lt) * 2011-09-16 2018-09-10 Lexogen Gmbh Nukleorūgščių molekulių bibliotekos sudarymo būdas
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN107083427B (zh) * 2016-04-01 2021-07-30 广州市基准医疗有限责任公司 Dna连接酶介导的dna扩增技术
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
CN113330122A (zh) 2018-11-16 2021-08-31 德皮克斯公司 使用位点特异性核酸酶优化核酸的体外分离

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120172258A1 (en) 2010-12-27 2012-07-05 Ibis Biosciences, Inc. Nucleic acid sample preparation methods and compositions
JP2017537657A (ja) 2014-12-11 2017-12-21 ニユー・イングランド・バイオレイブス・インコーポレイテツド 標的配列の濃縮
WO2018191702A2 (en) 2017-04-14 2018-10-18 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids

Also Published As

Publication number Publication date
US20230212663A1 (en) 2023-07-06
US20210123097A1 (en) 2021-04-29
JP2026067857A (ja) 2026-04-21
ES2971348T3 (es) 2024-06-04
WO2021081423A1 (en) 2021-04-29
US12534757B2 (en) 2026-01-27
EP4048812B1 (en) 2023-12-06
EP4048812A1 (en) 2022-08-31
JP2022553164A (ja) 2022-12-22
EP4345171A2 (en) 2024-04-03
EP4345171A3 (en) 2024-06-12
US11447819B2 (en) 2022-09-20

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