ES2971348T3 - Métodos de reparación de salientes 3' - Google Patents

Métodos de reparación de salientes 3' Download PDF

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Publication number
ES2971348T3
ES2971348T3 ES20807975T ES20807975T ES2971348T3 ES 2971348 T3 ES2971348 T3 ES 2971348T3 ES 20807975 T ES20807975 T ES 20807975T ES 20807975 T ES20807975 T ES 20807975T ES 2971348 T3 ES2971348 T3 ES 2971348T3
Authority
ES
Spain
Prior art keywords
dna fragment
dna
repaired
fragments
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES20807975T
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English (en)
Spanish (es)
Inventor
Eleen Shum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guardant Health Inc
Original Assignee
Guardant Health Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guardant Health Inc filed Critical Guardant Health Inc
Application granted granted Critical
Publication of ES2971348T3 publication Critical patent/ES2971348T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES20807975T 2019-10-25 2020-10-23 Métodos de reparación de salientes 3' Active ES2971348T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962926093P 2019-10-25 2019-10-25
PCT/US2020/057189 WO2021081423A1 (en) 2019-10-25 2020-10-23 Methods for 3' overhang repair

Publications (1)

Publication Number Publication Date
ES2971348T3 true ES2971348T3 (es) 2024-06-04

Family

ID=73452312

Family Applications (1)

Application Number Title Priority Date Filing Date
ES20807975T Active ES2971348T3 (es) 2019-10-25 2020-10-23 Métodos de reparación de salientes 3'

Country Status (5)

Country Link
US (2) US11447819B2 (https=)
EP (2) EP4048812B1 (https=)
JP (2) JP7850658B2 (https=)
ES (1) ES2971348T3 (https=)
WO (1) WO2021081423A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024137316A1 (en) 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US20030017081A1 (en) 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
US20040209299A1 (en) * 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
US20060078899A1 (en) 2004-10-12 2006-04-13 Scheffer Alicia F Methods and compositions for reducing label variation in array-based comparative genome hybridization assays
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CN103502463B (zh) 2010-12-27 2016-03-16 艾比斯生物科学公司 核酸样品的制备方法及组合物
LT2756098T (lt) * 2011-09-16 2018-09-10 Lexogen Gmbh Nukleorūgščių molekulių bibliotekos sudarymo būdas
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
WO2016093838A1 (en) * 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
CN107083427B (zh) * 2016-04-01 2021-07-30 广州市基准医疗有限责任公司 Dna连接酶介导的dna扩增技术
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
EP3885445B1 (en) 2017-04-14 2023-08-23 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
CN113330122A (zh) 2018-11-16 2021-08-31 德皮克斯公司 使用位点特异性核酸酶优化核酸的体外分离

Also Published As

Publication number Publication date
US20230212663A1 (en) 2023-07-06
US20210123097A1 (en) 2021-04-29
JP2026067857A (ja) 2026-04-21
WO2021081423A1 (en) 2021-04-29
US12534757B2 (en) 2026-01-27
EP4048812B1 (en) 2023-12-06
EP4048812A1 (en) 2022-08-31
JP2022553164A (ja) 2022-12-22
EP4345171A2 (en) 2024-04-03
EP4345171A3 (en) 2024-06-12
US11447819B2 (en) 2022-09-20
JP7850658B2 (ja) 2026-04-23

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