JP7813719B2 - 形質転換体並びにそれを用いるカロテノイド組成物の製造方法 - Google Patents

形質転換体並びにそれを用いるカロテノイド組成物の製造方法

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JP7813719B2
JP7813719B2 JP2022561924A JP2022561924A JP7813719B2 JP 7813719 B2 JP7813719 B2 JP 7813719B2 JP 2022561924 A JP2022561924 A JP 2022561924A JP 2022561924 A JP2022561924 A JP 2022561924A JP 7813719 B2 JP7813719 B2 JP 7813719B2
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gene
promoter
tag
carotenoid
capsanthin
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JPWO2022102595A1 (https=
Inventor
亜希子 久保
光司 大段
喜信 寺田
克郎 矢追
真衣子 古林
典彦 三沢
美保 小林
順一郎 八反
孝至 眞岡
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Ezaki Glico Co Ltd
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Ezaki Glico Co Ltd
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/1309—Zeaxanthin epoxidase (1.14.13.90)
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13129—Beta-carotene 3-hydroxylase (1.14.13.129)
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    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/99—Other intramolecular oxidoreductases (5.3.99)
    • C12Y503/99008—Capsanthin/capsorubin synthase (5.3.99.8)
    • C—CHEMISTRY; METALLURGY
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    • C12Y505/00—Intramolecular lyases (5.5)
    • C12Y505/01—Intramolecular lyases (5.5.1)
    • C12Y505/01019—Lycopene beta-cyclase (5.5.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99031—Phytoene desaturase (lycopene-forming) (1.3.99.31)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01029—Geranylgeranyl diphosphate synthase (2.5.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01032—15-Cis-phytoene synthase (2.5.1.32)

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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022561924A 2020-11-12 2021-11-09 形質転換体並びにそれを用いるカロテノイド組成物の製造方法 Active JP7813719B2 (ja)

Applications Claiming Priority (3)

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JP2020188462 2020-11-12
JP2020188462 2020-11-12
PCT/JP2021/041098 WO2022102595A1 (ja) 2020-11-12 2021-11-09 形質転換体並びにそれを用いるカロテノイド組成物の製造方法

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JPWO2022102595A1 JPWO2022102595A1 (https=) 2022-05-19
JP7813719B2 true JP7813719B2 (ja) 2026-02-13

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US (1) US20230416759A1 (https=)
EP (1) EP4245843A4 (https=)
JP (1) JP7813719B2 (https=)
CN (1) CN116457454A (https=)
WO (1) WO2022102595A1 (https=)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008532492A (ja) 2005-02-11 2008-08-21 カラマズー・ホールディングス・インコーポレイテッド ゼアキサンチンの過剰蓄積を示すトウガラシ属品種およびそこから得られる産物
US20140380516A1 (en) 2013-06-21 2014-12-25 Seminis Vegetable Seeds, Inc. Selection of mature fruit color in pepper plants

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5880332A (en) * 1994-03-01 1999-03-09 Centre National De La Recherche Scientifique DNA constructs related to capsanthin capsorubin synthase, cells and plants derived therefrom
KR101424123B1 (ko) * 2012-11-05 2014-08-20 대한민국 캡산틴을 생성하는 형질전환 컬러쌀 및 이의 제조방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008532492A (ja) 2005-02-11 2008-08-21 カラマズー・ホールディングス・インコーポレイテッド ゼアキサンチンの過剰蓄積を示すトウガラシ属品種およびそこから得られる産物
US20140380516A1 (en) 2013-06-21 2014-12-25 Seminis Vegetable Seeds, Inc. Selection of mature fruit color in pepper plants

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FURUBAYASHI, Maiko et al.，Journal of Agricultural and Food Chemistry，2021年，69，5076-5085
JEKNIC, Zoran et al.，Plant Cell Physiol，2012年，53(11)，1899-1912
KUMAGAI, H. Monto et al.，The Plant Journal，1998年，14(3)，305-315
LEMPENS, Edith H. M. et al.，Chem. Eur. J.，2009年，15，8760-8767

Also Published As

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JPWO2022102595A1 (https=) 2022-05-19
CN116457454A (zh) 2023-07-18
EP4245843A1 (en) 2023-09-20
EP4245843A4 (en) 2025-01-29
WO2022102595A1 (ja) 2022-05-19
US20230416759A1 (en) 2023-12-28

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