JP7778087B2 - Ｒｕｖｃドメインを有する酵素 - Google Patents

Ｒｕｖｃドメインを有する酵素

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Publication number
JP7778087B2
JP7778087B2 JP2022567462A JP2022567462A JP7778087B2 JP 7778087 B2 JP7778087 B2 JP 7778087B2 JP 2022567462 A JP2022567462 A JP 2022567462A JP 2022567462 A JP2022567462 A JP 2022567462A JP 7778087 B2 JP7778087 B2 JP 7778087B2
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Japan
Prior art keywords
seq
sequence
endonuclease
identity
nos
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JP2022567462A
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English (en)
Japanese (ja)
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JP2023533417A (ja
JP2023533417A5 (https=
Inventor
トーマス，ブライアン
ブラウン，クリストファー
カンター，ローズ
デヴォート，オードラ
バターフィールド，クリスティーナ
アレクサンダー，リサ
エス．エー． ゴルツマン，ダニエラ
リュー，ジェイソン
ラモット，レベッカ
エスピノサ，ディエゴ
ストーリー，メーガン
コスト，グレッグ
Original Assignee
メタゲノミ，インク．
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Publication of JP2023533417A publication Critical patent/JP2023533417A/ja
Publication of JP2023533417A5 publication Critical patent/JP2023533417A5/ja
Priority to JP2025027249A priority Critical patent/JP2025090617A/ja
Application granted granted Critical
Publication of JP7778087B2 publication Critical patent/JP7778087B2/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022567462A 2020-05-08 2021-05-06 Ｒｕｖｃドメインを有する酵素 Active JP7778087B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025027249A JP2025090617A (ja) 2020-05-08 2025-02-21 Ｒｕｖｃドメインを有する酵素

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US202063022320P 2020-05-08 2020-05-08
US63/022,320 2020-05-08
US202063032464P 2020-05-29 2020-05-29
US63/032,464 2020-05-29
US202063116155P 2020-11-19 2020-11-19
US63/116,155 2020-11-19
US202163180570P 2021-04-27 2021-04-27
US63/180,570 2021-04-27
PCT/US2021/031136 WO2021226363A1 (en) 2020-05-08 2021-05-06 Enzymes with ruvc domains

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025027249A Division JP2025090617A (ja) 2020-05-08 2025-02-21 Ｒｕｖｃドメインを有する酵素

Publications (3)

Publication Number Publication Date
JP2023533417A JP2023533417A (ja) 2023-08-03
JP2023533417A5 JP2023533417A5 (https=) 2024-03-14
JP7778087B2 true JP7778087B2 (ja) 2025-12-01

Family

ID=78468763

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022567462A Active JP7778087B2 (ja) 2020-05-08 2021-05-06 Ｒｕｖｃドメインを有する酵素
JP2025027249A Pending JP2025090617A (ja) 2020-05-08 2025-02-21 Ｒｕｖｃドメインを有する酵素

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025027249A Pending JP2025090617A (ja) 2020-05-08 2025-02-21 Ｒｕｖｃドメインを有する酵素

Country Status (10)

Country Link
US (1) US20240209332A1 (https=)
EP (2) EP4467642A3 (https=)
JP (2) JP7778087B2 (https=)
KR (1) KR20230021657A (https=)
CN (1) CN116096892A (https=)
AU (1) AU2021267379A1 (https=)
CA (1) CA3177828A1 (https=)
GB (1) GB2612458B (https=)
MX (1) MX2022014067A (https=)
WO (1) WO2021226363A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021178933A2 (en) 2020-03-06 2021-09-10 Metagenomi Ip Technologies, Llc Class ii, type v crispr systems
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
US10913941B2 (en) * 2019-02-14 2021-02-09 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
WO2021202568A1 (en) 2020-03-31 2021-10-07 Metagenomi Ip Technologies, Llc Class ii, type ii crispr systems
CA3205865A1 (en) * 2021-01-22 2022-07-28 Brian C. Thomas Novel engineered and chimeric nucleases
AU2022335499A1 (en) 2021-08-27 2024-02-22 Metagenomi Therapeutics, Inc. Enzymes with ruvc domains
AU2022396533A1 (en) 2021-11-24 2024-05-02 Metagenomi Therapeutics, Inc. Endonuclease systems
CA3243006A1 (en) * 2021-12-21 2025-02-27 Alia Therapeutics Srl TYPE II CAS PROTEINS AND THEIR APPLICATIONS
WO2023137451A1 (en) * 2022-01-14 2023-07-20 Arbor Biotechnologies, Inc. Compositions comprising an rna guide targeting cd38 and uses thereof
WO2023194359A1 (en) * 2022-04-04 2023-10-12 Alia Therapeutics Srl Compositions and methods for treatment of usher syndrome type 2a
CA3267752A1 (en) * 2022-09-16 2024-03-21 Alia Therapeutics Srl ENQP TYPE II CAS PROTEINS AND THEIR APPLICATIONS

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019507599A (ja) 2016-03-04 2019-03-22 エディタス・メディシン、インコーポレイテッド がん免疫療法のためのｃｒｉｓｐｒ−ｃｐｆ１関連方法、組成物および構成要素
WO2019097305A2 (en) 2017-05-12 2019-05-23 Crispr Therapeutics Ag Materials and methods for engineering cells and uses thereof in immuno-oncology
US20190264232A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019200306A1 (en) 2018-04-13 2019-10-17 Sigma-Aldrich Co. Llc Modification of immune-related genomic loci using paired crispr nickase ribonucleoproteins
WO2020081613A1 (en) 2018-10-16 2020-04-23 Intellia Therapeutics, Inc. Compositions and methods for immunotherapy

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015161276A2 (en) * 2014-04-18 2015-10-22 Editas Medicine, Inc. Crispr-cas-related methods, compositions and components for cancer immunotherapy
WO2017093969A1 (en) * 2015-12-04 2017-06-08 Novartis Ag Compositions and methods for immunooncology
US20190136230A1 (en) * 2016-05-06 2019-05-09 Juno Therapeutics, Inc. Genetically engineered cells and methods of making the same
EP3500967A1 (en) * 2016-08-17 2019-06-26 The Broad Institute, Inc. Methods for identifying class 2 crispr-cas systems
WO2018073393A2 (en) * 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
EP3617311B1 (en) * 2017-03-30 2024-11-20 Kyoto University Method for inducing exon skipping by genome editing
US9982279B1 (en) * 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
EP3724326A1 (en) * 2017-12-11 2020-10-21 Editas Medicine, Inc. Cpf1-related methods and compositions for gene editing
CA3094468A1 (en) * 2018-04-05 2019-10-10 Juno Therapeutics, Inc. Methods of producing cells expressing a recombinant receptor and related compositions
CA3241703C (en) * 2019-02-14 2026-02-24 Metagenomi Therapeutics, Inc. Enzymes with ruvc domains
JP2024515936A (ja) * 2021-03-19 2024-04-11 メタゲノミ，インク． Ｃａｓ酵素を用いたマルチプレックス編集

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019507599A (ja) 2016-03-04 2019-03-22 エディタス・メディシン、インコーポレイテッド がん免疫療法のためのｃｒｉｓｐｒ−ｃｐｆ１関連方法、組成物および構成要素
WO2019097305A2 (en) 2017-05-12 2019-05-23 Crispr Therapeutics Ag Materials and methods for engineering cells and uses thereof in immuno-oncology
US20190264232A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019200306A1 (en) 2018-04-13 2019-10-17 Sigma-Aldrich Co. Llc Modification of immune-related genomic loci using paired crispr nickase ribonucleoproteins
WO2020081613A1 (en) 2018-10-16 2020-04-23 Intellia Therapeutics, Inc. Compositions and methods for immunotherapy

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
UniProtKB [online], Accession No.A0A1F0KNW4, Definition: HNHc domain-containing protein，22-APR-2020 uploaded, [2024年4月11日検索], <https://rest.uniprot.org/unisave/A0A1F0KNW4?format=txt&versions=13>
UniProtKB [online], Accession No.A0A1F0PN46, Definition: HNH Cas9-type domain-containing protein，22-APR-2020 uploaded, [2024年4月11日検索], <https://rest.uniprot.org/unisave/A0A1F0PN46?format=txt&versions=14>
UniProtKB [online], Accession No.A0A1S1DAD0, Definition: HNH Cas9-type domain-containing protein，22-APR-2020 uploaded, [2024年4月11日検索], <https://rest.uniprot.org/unisave/A0A1S1DAD0?format=txt&versions=12>

Also Published As

Publication number Publication date
KR20230021657A (ko) 2023-02-14
GB202217378D0 (en) 2023-01-04
CA3177828A1 (en) 2021-11-11
CN116096892A (zh) 2023-05-09
AU2021267379A1 (en) 2022-12-08
EP4467642A2 (en) 2024-11-27
EP4146800A1 (en) 2023-03-15
JP2023533417A (ja) 2023-08-03
GB2612458B (en) 2025-04-09
EP4467642A3 (en) 2025-04-16
WO2021226363A1 (en) 2021-11-11
MX2022014067A (es) 2023-02-22
JP2025090617A (ja) 2025-06-17
US20240209332A1 (en) 2024-06-27
GB2612458A (en) 2023-05-03

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