JP7756726B2 - タバコ植物 - Google Patents

タバコ植物

Info

Publication number
JP7756726B2
JP7756726B2 JP2023570958A JP2023570958A JP7756726B2 JP 7756726 B2 JP7756726 B2 JP 7756726B2 JP 2023570958 A JP2023570958 A JP 2023570958A JP 2023570958 A JP2023570958 A JP 2023570958A JP 7756726 B2 JP7756726 B2 JP 7756726B2
Authority
JP
Japan
Prior art keywords
seq
amino acid
gene
tobacco
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023570958A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2023127723A1 (https=
JPWO2023127723A5 (https=
Inventor
庄一 鈴木
久史 宇田川
大志 平瀬
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tobacco Inc
Original Assignee
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Publication of JPWO2023127723A1 publication Critical patent/JPWO2023127723A1/ja
Publication of JPWO2023127723A5 publication Critical patent/JPWO2023127723A5/ja
Application granted granted Critical
Publication of JP7756726B2 publication Critical patent/JP7756726B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823Nicotiana, e.g. tobacco
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B13/00Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10Chemical features of tobacco products or tobacco substitutes
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/20Biochemical treatment
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
JP2023570958A 2021-12-27 2022-12-23 タバコ植物 Active JP7756726B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021212001 2021-12-27
JP2021212001 2021-12-27
PCT/JP2022/047603 WO2023127723A1 (ja) 2021-12-27 2022-12-23 タバコ植物

Publications (3)

Publication Number Publication Date
JPWO2023127723A1 JPWO2023127723A1 (https=) 2023-07-06
JPWO2023127723A5 JPWO2023127723A5 (https=) 2024-09-26
JP7756726B2 true JP7756726B2 (ja) 2025-10-20

Family

ID=86999115

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023570958A Active JP7756726B2 (ja) 2021-12-27 2022-12-23 タバコ植物

Country Status (6)

Country Link
US (1) US20240360463A1 (https=)
EP (1) EP4458140A4 (https=)
JP (1) JP7756726B2 (https=)
KR (1) KR20240112979A (https=)
CN (1) CN118475234A (https=)
WO (1) WO2023127723A1 (https=)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016508032A (ja) 2012-12-21 2016-03-17 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物体中のたばこ特異的ニトロソアミンの低減
JP2017520249A (ja) 2014-06-25 2017-07-27 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物体中の硝酸塩含有量の調節
CN108841834A (zh) 2018-06-27 2018-11-20 中国烟草总公司郑州烟草研究院 一个烟草氯离子通道蛋白NtCLC2及其应用
WO2021215465A1 (ja) 2020-04-21 2021-10-28 日本たばこ産業株式会社 タバコ植物体とその製造方法
CN113774069A (zh) 2021-11-03 2021-12-10 云南省烟草农业科学研究院 一种烟草NtCLC-f基因突变体及分子鉴定方法和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201204862D0 (en) * 2012-03-20 2012-05-02 Cambridge Advanced Tech Transgenic plants
JP7210806B2 (ja) * 2020-04-17 2023-01-23 日本たばこ産業株式会社 低アルカロイド含量のタバコ属植物体およびその製造方法
JP7696368B2 (ja) 2020-12-09 2025-06-20 日本たばこ産業株式会社 タバコ植物及びたばこ製品

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016508032A (ja) 2012-12-21 2016-03-17 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物体中のたばこ特異的ニトロソアミンの低減
JP2017520249A (ja) 2014-06-25 2017-07-27 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物体中の硝酸塩含有量の調節
CN108841834A (zh) 2018-06-27 2018-11-20 中国烟草总公司郑州烟草研究院 一个烟草氯离子通道蛋白NtCLC2及其应用
WO2021215465A1 (ja) 2020-04-21 2021-10-28 日本たばこ産業株式会社 タバコ植物体とその製造方法
CN113774069A (zh) 2021-11-03 2021-12-10 云南省烟草农业科学研究院 一种烟草NtCLC-f基因突变体及分子鉴定方法和应用

Also Published As

Publication number Publication date
KR20240112979A (ko) 2024-07-19
JPWO2023127723A1 (https=) 2023-07-06
CN118475234A (zh) 2024-08-09
US20240360463A1 (en) 2024-10-31
EP4458140A4 (en) 2025-10-15
EP4458140A1 (en) 2024-11-06
WO2023127723A1 (ja) 2023-07-06

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