JP7742874B2 - 能動補償を用いる電気穿孔 - Google Patents

能動補償を用いる電気穿孔

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Publication number
JP7742874B2
JP7742874B2 JP2023504334A JP2023504334A JP7742874B2 JP 7742874 B2 JP7742874 B2 JP 7742874B2 JP 2023504334 A JP2023504334 A JP 2023504334A JP 2023504334 A JP2023504334 A JP 2023504334A JP 7742874 B2 JP7742874 B2 JP 7742874B2
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JP
Japan
Prior art keywords
electrodes
value
resistance
current
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023504334A
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English (en)
Japanese (ja)
Other versions
JP2023537253A (ja
JP2023537253A5 (OSRAM
Inventor
チャン，チー－ウェイ
リュー，ジョン トーマス ヴァン
メーラビ，アーティン
Original Assignee
ナントセル，インコーポレーテッド
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Application filed by ナントセル，インコーポレーテッド filed Critical ナントセル，インコーポレーテッド
Publication of JP2023537253A publication Critical patent/JP2023537253A/ja
Publication of JP2023537253A5 publication Critical patent/JP2023537253A5/ja
Application granted granted Critical
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/08—Arrangements or circuits for monitoring, protecting, controlling or indicating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/0404—Electrodes for external use
    • A61N1/0472—Structure-related aspects
    • A61N1/0476—Array electrodes (including any electrode arrangement with more than one electrode for at least one of the polarities)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/36—Applying electric currents by contact electrodes alternating or intermittent currents for stimulation
    • A61N1/372—Arrangements in connection with the implantation of stimulators
    • A61N1/378—Electrical supply
    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Radiology & Medical Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
JP2023504334A 2020-07-22 2021-07-15 能動補償を用いる電気穿孔 Active JP7742874B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/935,987 2020-07-22
US16/935,987 US12006507B2 (en) 2020-07-22 2020-07-22 Electroporation with active compensation
PCT/US2021/041832 WO2022020175A1 (en) 2020-07-22 2021-07-15 Electroporation with active compensation

Publications (3)

Publication Number Publication Date
JP2023537253A JP2023537253A (ja) 2023-08-31
JP2023537253A5 JP2023537253A5 (OSRAM) 2024-05-16
JP7742874B2 true JP7742874B2 (ja) 2025-09-22

Family

ID=77367476

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023504334A Active JP7742874B2 (ja) 2020-07-22 2021-07-15 能動補償を用いる電気穿孔

Country Status (6)

Country Link
US (2) US12006507B2 (OSRAM)
EP (1) EP4185686A1 (OSRAM)
JP (1) JP7742874B2 (OSRAM)
KR (1) KR102913102B1 (OSRAM)
CN (1) CN115812098A (OSRAM)
WO (1) WO2022020175A1 (OSRAM)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD1016324S1 (en) 2020-07-08 2024-02-27 NanoCav, LLC Biological cell processing chip
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation
EP4377336A4 (en) 2021-07-29 2025-07-16 Nantcell Inc MODIFIED T CELL RECEPTORS FOR THE PREVENTION AND TREATMENT OF VIRAL INFECTIONS AND CANCER
CN115449481A (zh) * 2022-09-26 2022-12-09 杭州维纳安可医疗科技有限责任公司 电转染装置及方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006112870A1 (en) 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
US20190292510A1 (en) 2018-03-20 2019-09-26 The Charles Stark Draper Laboratory, Inc. Dual-Purpose Viral Transduction and Electroporation Device

Family Cites Families (24)

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Publication number Priority date Publication date Assignee Title
US5545130A (en) 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US6403348B1 (en) 1999-07-21 2002-06-11 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US6387671B1 (en) 1999-07-21 2002-05-14 The Regents Of The University Of California Electrical impedance tomography to control electroporation
US6300108B1 (en) 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
DK2574662T3 (da) 2001-08-22 2021-09-20 Maxcyte Inc Fremgangsmåde til elektroporation af biologiske prøver
US7245963B2 (en) 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US6801804B2 (en) 2002-05-03 2004-10-05 Aciont, Inc. Device and method for monitoring and controlling electrical resistance at a tissue site undergoing iontophoresis
PT3524316T (pt) 2006-10-17 2024-11-29 Inovio Pharmaceuticals Inc Dispositivos de eletroporação para eletroporação de células em mamíferos
US8222909B2 (en) 2008-08-22 2012-07-17 Bio-Rad Laboratories, Inc. Electroporation system with dial-in time constant
CN101857836B (zh) 2009-11-10 2013-06-12 昆山文曲生物微系统有限公司 一种流式电穿孔装置及系统
CN203451533U (zh) 2013-08-27 2014-02-26 苏州文曲生物微系统有限公司 一种基于新型微电极芯片的电穿孔系统
WO2016070100A1 (en) * 2014-10-31 2016-05-06 The Regents Of The University Of California Neural circuit probe
US11318305B2 (en) * 2015-03-31 2022-05-03 Oncosec Medical Incorporated Systems and methods for improved tissue-sensing based electroporation
BR112018004278B1 (pt) 2015-09-04 2022-05-03 Rutgers, The State University Of New Jersey Sistema para eletroporação de uma célula biológica, e método para eletroporação de células biológicas em uma solução tampão
ES2687422T3 (es) * 2015-09-07 2018-10-25 Miltenyi Biotec Gmbh Cartucho desechable para electroporación
WO2017176764A1 (en) 2016-04-04 2017-10-12 CyteQuest, Inc. System, device and method for electroporation of cells
US20170291172A1 (en) 2016-04-11 2017-10-12 The Regents Of The University Of California Tunable, sheathless, and three dimensional single-stream cell focusing and sorting in high speed flows
KR102392142B1 (ko) 2016-10-21 2022-04-28 알토 바이오사이언스 코포레이션 다량체 il-15 기반 분자
WO2018094249A1 (en) 2016-11-18 2018-05-24 NanoCav, LLC Methods of sorting activated t cells using three dimensional dielectrophoresis
WO2019068022A1 (en) * 2017-09-30 2019-04-04 Inscripta, Inc. CONTINUOUS FLOW ELECTROPORATION INSTRUMENTATION
AU2019301062A1 (en) 2018-07-09 2021-02-04 NanoCav, LLC Micro flow-through electroporation devices and methods of cell transfection
EP3915633A1 (en) * 2020-05-29 2021-12-01 Imec VZW Control of cell electroporation
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006112870A1 (en) 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
US20190292510A1 (en) 2018-03-20 2019-09-26 The Charles Stark Draper Laboratory, Inc. Dual-Purpose Viral Transduction and Electroporation Device

Also Published As

Publication number Publication date
JP2023537253A (ja) 2023-08-31
WO2022020175A1 (en) 2022-01-27
US12006507B2 (en) 2024-06-11
CN115812098A (zh) 2023-03-17
KR20230038733A (ko) 2023-03-21
KR102913102B1 (ko) 2026-01-14
EP4185686A1 (en) 2023-05-31
US20240229074A1 (en) 2024-07-11
US20220025402A1 (en) 2022-01-27
US12571003B2 (en) 2026-03-10

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