JP7736065B2 - 細胞画像解析方法 - Google Patents

細胞画像解析方法

Info

Publication number
JP7736065B2
JP7736065B2 JP2023538552A JP2023538552A JP7736065B2 JP 7736065 B2 JP7736065 B2 JP 7736065B2 JP 2023538552 A JP2023538552 A JP 2023538552A JP 2023538552 A JP2023538552 A JP 2023538552A JP 7736065 B2 JP7736065 B2 JP 7736065B2
Authority
JP
Japan
Prior art keywords
cell
image
cell image
value
trained model
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023538552A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2023008428A1 (https=
Inventor
隆二 澤田
周平 山本
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Publication of JPWO2023008428A1 publication Critical patent/JPWO2023008428A1/ja
Application granted granted Critical
Publication of JP7736065B2 publication Critical patent/JP7736065B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/34Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/0002Inspection of images, e.g. flaw detection
    • G06T7/0012Biomedical image inspection
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/10Segmentation; Edge detection
    • G06T7/143Segmentation; Edge detection involving probabilistic approaches, e.g. Markov random field [MRF] modelling
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/10Image acquisition modality
    • G06T2207/10056Microscopic image
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/20Special algorithmic details
    • G06T2207/20076Probabilistic image processing
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/20Special algorithmic details
    • G06T2207/20081Training; Learning
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/20Special algorithmic details
    • G06T2207/20084Artificial neural networks [ANN]
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/30Subject of image; Context of image processing
    • G06T2207/30004Biomedical image processing
    • G06T2207/30024Cell structures in vitro; Tissue sections in vitro

Landscapes

  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medical Informatics (AREA)
  • Software Systems (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Probability & Statistics with Applications (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Image Processing (AREA)
  • Image Analysis (AREA)
JP2023538552A 2021-07-29 2022-07-26 細胞画像解析方法 Active JP7736065B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021124772 2021-07-29
JP2021124772 2021-07-29
PCT/JP2022/028777 WO2023008428A1 (ja) 2021-07-29 2022-07-26 細胞画像解析方法

Publications (2)

Publication Number Publication Date
JPWO2023008428A1 JPWO2023008428A1 (https=) 2023-02-02
JP7736065B2 true JP7736065B2 (ja) 2025-09-09

Family

ID=85087711

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023538552A Active JP7736065B2 (ja) 2021-07-29 2022-07-26 細胞画像解析方法

Country Status (4)

Country Link
US (1) US20240370998A1 (https=)
JP (1) JP7736065B2 (https=)
CN (1) CN117597430A (https=)
WO (1) WO2023008428A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025187109A1 (ja) * 2024-03-08 2025-09-12 株式会社日立国際電気 データ収集装置、及び映像システム

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019171546A1 (ja) 2018-03-08 2019-09-12 株式会社島津製作所 細胞画像解析方法、細胞画像解析装置、及び学習モデル作成方法
WO2020031243A1 (ja) 2018-08-06 2020-02-13 株式会社島津製作所 教師ラベル画像修正方法、学習済みモデルの作成方法および画像解析装置
JP2020531971A (ja) 2017-08-15 2020-11-05 シーメンス ヘルスケア ゲゼルシヤフト ミツト ベシユレンクテル ハフツング コンボリューショナル・ニューラルを用いた、ホログラフィック顕微鏡で取得した細胞画像の品質の識別方法
WO2020241772A1 (ja) 2019-05-31 2020-12-03 国立大学法人京都大学 情報処理装置、スクリーニング装置、情報処理方法、スクリーニング方法、及びプログラム
WO2021009906A1 (ja) 2019-07-18 2021-01-21 株式会社島津製作所 細胞画像解析方法および細胞画像解析装置
JP2021018459A (ja) 2019-07-17 2021-02-15 オリンパス株式会社 評価支援方法、評価支援システム、プログラム
WO2021059572A1 (ja) 2019-09-27 2021-04-01 富士フイルム株式会社 情報処理装置、情報処理装置の作動方法、情報処理装置の作動プログラム

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020531971A (ja) 2017-08-15 2020-11-05 シーメンス ヘルスケア ゲゼルシヤフト ミツト ベシユレンクテル ハフツング コンボリューショナル・ニューラルを用いた、ホログラフィック顕微鏡で取得した細胞画像の品質の識別方法
WO2019171546A1 (ja) 2018-03-08 2019-09-12 株式会社島津製作所 細胞画像解析方法、細胞画像解析装置、及び学習モデル作成方法
WO2020031243A1 (ja) 2018-08-06 2020-02-13 株式会社島津製作所 教師ラベル画像修正方法、学習済みモデルの作成方法および画像解析装置
WO2020241772A1 (ja) 2019-05-31 2020-12-03 国立大学法人京都大学 情報処理装置、スクリーニング装置、情報処理方法、スクリーニング方法、及びプログラム
JP2021018459A (ja) 2019-07-17 2021-02-15 オリンパス株式会社 評価支援方法、評価支援システム、プログラム
WO2021009906A1 (ja) 2019-07-18 2021-01-21 株式会社島津製作所 細胞画像解析方法および細胞画像解析装置
WO2021059572A1 (ja) 2019-09-27 2021-04-01 富士フイルム株式会社 情報処理装置、情報処理装置の作動方法、情報処理装置の作動プログラム

Also Published As

Publication number Publication date
WO2023008428A1 (ja) 2023-02-02
JPWO2023008428A1 (https=) 2023-02-02
CN117597430A (zh) 2024-02-23
US20240370998A1 (en) 2024-11-07

Similar Documents

Publication Publication Date Title
US12331277B2 (en) Method and system for automated microbial colony counting from streaked sample on plated media
CN110853022B (zh) 病理切片图像的处理方法、装置、系统及存储介质
US7268939B1 (en) Tracking of cells with a compact microscope imaging system with intelligent controls
CN110647875B (zh) 一种血细胞分割、识别模型构造的方法及血细胞识别方法
JP5174040B2 (ja) 画像の構成要素と背景とを区別するためにコンピュータで実行される方法および画像の構成要素と背景とを区別するためのシステム
EP3874451B1 (en) Segmenting 3d intracellular structures in microscopy images using an iterative deep learning workflow that incorporates human contributions
Quinn et al. Automated blood smear analysis for mobile malaria diagnosis
JP5481696B2 (ja) 受精卵品質評価支援システム、受精卵品質評価支援装置および受精卵品質評価支援方法
US10007834B2 (en) Detection control device, detection system, non-transitory storage medium, and detection control method
CN111051955A (zh) 通过使用卷积神经网络来标识利用数字全息显微镜所获取的细胞图像的品质
KR20070097053A (ko) 개선된 이미지 분할 방법
CN117058752B (zh) 一种基于改进YOLOv7的学生课堂行为检测方法
US20240071057A1 (en) Microscopy System and Method for Testing a Sensitivity of an Image Processing Model
US20250078544A1 (en) Cell counting or cell confluence with rescaled input images
CN113096080A (zh) 图像分析方法及系统
JP7736065B2 (ja) 細胞画像解析方法
Liao et al. A clinical consensus-compliant deep learning approach to quantitatively evaluate human in vitro fertilization early embryonic development with optical microscope images
US8467590B2 (en) Microscopy analysis technique
JP7687406B2 (ja) 細胞画像解析方法
EP4379676B1 (en) Detection system, detection apparatus, learning apparatus, detection method, learning method and program
Spoorthy et al. Automatic identification of mixed retinal cells in time-lapse fluorescent microscopy images using high-dimensional dbscan
Beaver et al. Segmentation of nuclei in confocal image stacks using performance based thresholding
Wiers Label-efficient segmentation of organoid culture data using diffusion models
CN107977955A (zh) 一种基于层级结构信息确定细胞粘连情况的方法及系统
CN107977978A (zh) 一种基于椭圆轴关系确定细胞粘连方式的方法及系统

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20231030

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20241217

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20250213

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250402

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250729

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250811

R150 Certificate of patent or registration of utility model

Ref document number: 7736065

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150