JP7464310B2 - Wntシグナル伝達経路の抑制剤 - Google Patents
Wntシグナル伝達経路の抑制剤 Download PDFInfo
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Description
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチドを含有する、Wntシグナル伝達経路の抑制剤。
(e)配列番号6~9のいずれかに示されるアミノ酸配列、又は
(f)配列番号6~9のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
である、項5に記載の抑制剤。
(i)配列番号12~21のいずれかに示されるアミノ酸配列、又は
(j)配列番号12~21のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
である、項1~7のいずれかに記載の抑制剤。
(i’)配列番号12、17、20若しくは21に示されるアミノ酸配列、又は
(j’)配列番号12、17、20若しくは21に示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
である、項1~7のいずれかに記載の抑制剤。
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチドを含有する、線維化疾患の予防又は治療剤。
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチドを含有する、がんの予防又は治療剤。
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチドを細胞と接触させることを含む、Wntシグナル伝達経路の抑制方法。
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチドを、線維化疾患又はがんを有する患者に投与することを含む、線維化疾患又はがんの予防又は治療方法。
RANKLタンパク質DEループ配列、及び該DEループ配列のN末端側に隣接して配置されたRANKLタンパク質βストランドD配列を含むアミノ酸配列からなり、
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチド。
RANKLタンパク質DEループ配列、及び該DEループ配列のN末端側に隣接して配置されたRANKLタンパク質βストランドD配列を含むアミノ酸配列からなり、
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチド。
RANKLタンパク質DEループ配列、及び該DEループ配列のN末端側に隣接して配置されたRANKLタンパク質βストランドD配列を含むアミノ酸配列からなり、
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチドの使用。
RANKLタンパク質DEループ配列、及び該DEループ配列のN末端側に隣接して配置されたRANKLタンパク質βストランドD配列を含むアミノ酸配列からなり、
前記DEループ配列が下記(a)又は(b):
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であり、
前記βストランドD配列が下記(c)又は(d):
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列
であるオリゴペプチドの使用。
本明細書において、アミノ酸配列の表記は全て一文字表記で表わす。
以下に、RANKLタンパク質DEループ配列、及び該DEループ配列のN末端側に隣接して配置されたRANKLタンパク質βストランドD配列を含むアミノ酸配列からなるオリゴペプチド(本明細書において、「本発明のオリゴペプチド」と示すこともある。)について説明する。
(a)配列番号1に示されるアミノ酸配列、又は
(b)配列番号1に示されるアミノ酸配列に対して1個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列。
(c)配列番号2~5のいずれかに示されるアミノ酸配列、又は
(d)配列番号2~5のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列。
(e)配列番号6~9のいずれかに示されるアミノ酸配列、又は
(f)配列番号6~9のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列。
(i)配列番号12~21のいずれかに示されるアミノ酸配列、又は
(j)配列番号12~21のいずれかに示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列。
(i’)配列番号12、17、20若しくは21に示されるアミノ酸配列、又は
(j’)配列番号12、17、20若しくは21に示されるアミノ酸配列に対して1~3個のアミノ酸が置換、欠失、付加又は挿入されたアミノ酸配列。
本発明のオリゴペプチドは、線維化疾患又はがんの予防又は治療効果、Wntシグナル伝達経路の抑制効果、LGR4とRSPO(例えばRSPO1、RSPO2、RSPO3等)との結合抑制効果、上皮間葉転換(EMT)抑制効果を有することから、線維化疾患又はがんの予防又は治療剤、Wntシグナル伝達経路の抑制剤、LGR4とRSPO1との結合抑制剤、上皮間葉転換抑制剤等の有効成分として利用することができる。
表1に示すアミノ酸配列のオリゴペプチドをILS株式会社に委託して合成した。MHP24-AcN及びMHP24h-AcN以外は、N末端及びC末端の修飾は無く、N末端がアミノ基であり、C末端がカルボキシ基である。MHP24-AcN及びMHP24h-AcNは、N末端がアセチル化され且つC末端がアミド化されてなるオリゴペプチドである。なお、これらのペプチドにおけるアミノ酸残基は、全てL体である。
RANKLペプチドがWntシグナルに与える影響を解析した。具体的には、次のようにして行った。
RANKLペプチドとLGR4との結合について、及びRANKLペプチドがLGR4とそのリガンドであるRSPO1との結合に与える影響について解析した。具体的には、次のようにして行った。
ブレオマイシン誘発強皮症モデルを作製し、その症状が、RANKLペプチド早期投与によって改善されるかどうかを調べた。具体的には次のように行った。
ブレオマイシン誘発強皮症モデルを作製し、その症状が、RANKLペプチド晩期投与によって改善されるかどうかを調べた。具体的には次のように行った。
ブレオマイシン誘発肺線維症モデルを作製し、その症状が、RANKLペプチド投与によって改善されるかどうかを調べた。具体的には次のように行った。
RANKLペプチドのがんへの影響を解析した。
A549細胞(ヒト肺胞基底上皮腺癌細胞:LGR4とRSPO3の両方とも高発現していることを確認済)の増殖に対するRANKLペプチドの影響を調べた。具体的には、次のようにして行った。A549細胞にMHP1を1 ng/mlから100 μg/mlまで投与し、48時間後にMTT assayにより細胞生存率を算出した。
A549細胞のMigration及びInvasionに対するRANKLペプチドの影響を調べた。具体的には、次のようにして行った。 Boyden chamber (48-well Chamber, Neuro Probe)の上段に、8-μm polycarbonate membrane filter (Neuro Probe)のチャンバーを設置し、MHP1-AcNが含まれる無血清のDMEMにてA549細胞をまき、下段に10% FBSを含むDMEMを入れて、37度で15時間インキュベーションした。Invasion assayでは、上段はMatrigel Invasion chamber (Corning Life Science)とし、同様にMHP1-AcNが含まれる無血清培にてA549細胞をまき、下段には10% FBSを含むDMEMを入れた。それぞれの上段の上面に接着している細胞を剥離し、下面をDiff-Quick (Sysmex)にて染色し、顕微鏡にて細胞数をカウントした。
A549細胞のxenograftモデルのがんに対するRANKLペプチドの影響を調べた。具体的には、次のようにして行った。2X106の細胞をBALB/cAJcl- nu/nu細胞に皮下投与し、1日後より、MHP1を600 μg/マウスで連日投与し、49日まで腫瘍のサイズを計測した。また、腫瘍の重量を計測した。
RANKLペプチドのEMT機構への影響を解析した。
TGF-βにより誘導されるsmad2/3活性化に対するRANKLペプチドの影響を調べた。具体的には、次のようにして行った。MRC-5細胞をMHP1で24時間前処理後、TGFβ 5 ng/mlを培地に加え、10分後に培地を回収し、WBを行った。
TGF-β受容体の発現に対するRANKLペプチドの影響を調べた。具体的には、次のようにして行った。MRC-5細胞の培地中にMHP1を加え、24時間後に細胞を回収し、realtime RT-PCRでTβR1、TβR2の発現を解析した。
TGF-βにより誘導されるα-SMA及びCol1a1の発現に対するRANKLペプチドの影響を調べた。具体的には、次のようにして行った。MRC-5細胞をMHP1で24時間前処理後、TGFβを2 ng/mlで添加、24時間後に細胞を回収し、realtime RT-PCRでmRNA量を計測した。
Claims (7)
- 配列番号12又は17に示されるアミノ酸配列を含むアミノ酸配列からなるオリゴペプチドを含有する、Wntシグナル伝達経路の抑制剤。
- 前記オリゴペプチドのアミノ酸配列が、RANKLタンパク質CDループ配列を含まない、請求項1に記載の抑制剤。
- 前記オリゴペプチドが、N末端がアセチル化され且つC末端がアミド化されてなるオリゴペプチドである、請求項1又は2に記載の抑制剤。
- 前記オリゴペプチドの長さが50アミノ酸残基長以下である、請求項1~3のいずれかに記載の抑制剤。
- 前記オリゴペプチドの長さが40アミノ酸残基長以下である、請求項4に記載の抑制剤。
- 線維化疾患の予防又は治療に用いるため、或いはがんの予防又は治療に用いるための、請求項1~5のいずれかに記載の抑制剤。
- 前記線維化疾患が皮膚及び肺からなる群より選択される少なくとも1種における線維化疾患であり、且つ前記がんが肺がんである、請求項6に記載の抑制剤。
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LA MANNA et al.,Peptides as Therapeutic Agents for Inflammatory-Related Disease,International Journal of Molecular Sciences,2018年,Vol. 19, No. 9,page 2714 |
柴田 昌彦,がんの進展における炎症と宿主免疫能の関与の重要性,日本外科系連合学会誌,2011年,Vol. 36, No. 6,pages 1043-1045,10.4030/jjcs.36.1043 |
西岡 清,免疫と炎症と硬化,日本臨床免疫学会会誌,1994年,Vol. 17, No. 6,pages 688-690,10.2177/jsci.17.688 |
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