JP7450194B1 - A multi-stage circulation filtration culture method for different species that utilizes the low temperature of deep ocean water. - Google Patents

Abstract

[Problem] To provide a multi-stage circulating filtration type aquaculture method for different kinds of organisms utilizing the low temperature of deep sea water. [Solution] The present invention provides a multi-stage aquaculture method using deep sea water, in which deep sea water taken in is supplied to a first aquaculture tank, where cold-sea crustaceans are cultured at a water temperature of 3 to 5°C, the breeding water discharged from the first aquaculture tank is supplied to a second aquaculture tank, where cold-sea seaweeds are cultured at a water temperature of 10 to 12°C, the breeding water discharged from the second aquaculture tank is supplied to a third aquaculture tank, where salmon are cultured at a water temperature of 14 to 16°C in a circulating filtration aquaculture system, and the breeding water recirculated from the third aquaculture tank is supplied to a fourth aquaculture tank, where sea cucumbers are cultured at a water temperature of 16 to 18°C. This makes it possible to breed target organisms such as cold-sea aquatic resource salmon, seaweed, and sea cucumbers in a low water temperature breeding environment using deep sea water, and makes it possible to produce different kinds of organisms all year round. [Selected figure] Figure 2

Description

The present invention relates to a multi-stage circulation filtration culture method for different species of organisms that utilizes the low-temperature nature of deep ocean water, and in particular, utilizes the low-temperature nature of deep ocean water to adapt to the appropriate water temperature and water quality environment for the cultivated organisms. This paper relates to a multi-stage aquaculture method using deep ocean water that allows for modularization of each stage of aquaculture methods to facilitate farm operation and energy savings.

Deep ocean water is seawater that does not mix with surface seawater, which is usually at a depth of 200 meters or more where sunlight does not reach, and refers to deep currents that have circulated around the earth for approximately 1,200 to 2,000 years. . Deep ocean water makes up about 95% of the seawater on Earth, and is used not only in the marine industry, but also in food, medicine, the health industry, drinking water, cosmetics, and other fields.

While the temperature of surface seawater fluctuates significantly depending on the season, deep ocean water does not change in temperature depending on the season and has stable low temperature stability. In the deep layer, it is less susceptible to pollution from terrestrial river water and the atmosphere, and has cleanliness with less chemicals, pollutants, and bacteria. Deep ocean water is eutrophic, containing about 5 to 10 times more inorganic nutrients, including nitrogen, phosphorus, and silicon, which are nutritional sources for phytoplankton than surface seawater.
In addition, it is rich in minerals containing a wide variety of elements, has a lower pH than surface seawater, has a lower organic matter content, and has the ability to mature by being separated from surface seawater and aged for a long period of time under low temperature and high pressure.

Seafood products are being farmed all over the world, and the amount of domestic aquaculture is increasing.
By taking advantage of the low temperature, eutrophic, and high mineral content of deep ocean water, we can cultivate new business fields in which we can cultivate high value-added cold-sea fish species that have been difficult to cultivate in the past. New potential industries can be developed. Research in the field of fisheries using deep ocean water has already been conducted overseas since the 1970s, and industrialization is already underway in the United States, Taiwan, and Norway.

Republic of Korea Patent Registration No. 10-0861134 discloses a seaweed cultivation method using deep ocean water, and Republic of Korea Patent Registration No. 10-1578927 discloses a seaweed cultivation method; It is limited to seed cultivation methods only.
In addition, current industrial use in the fisheries field is for livestock farming, where cold-sea fish species such as crabs are stored for a while.
Therefore, the present invention provides a system in which various high-value-added fish species can be cultivated step by step using deep ocean water that has characteristics such as low temperature, eutrophicity, and high mineral content.

Korean registered patent number 10-0861134 Korean registered patent number 10-1578927 Korean registered patent number 10-0789389

The present invention utilizes the low-temperature nature of deep ocean water to modularize aquaculture methods for each stage to suit the appropriate water temperature and water quality environment of the cultured organisms, making it possible to raise a variety of aquaculture fish species at different water temperatures. To provide a multi-stage aquaculture method that enables the operation of a farm and energy saving for breeding various fish species using deep ocean water taken from the ocean.

The multi-stage circulation filtration culture method for cultivating different species of organisms using the low-temperature properties of deep ocean water according to the present invention involves supplying the taken deep ocean water to a first aquaculture tank, and raising the water temperature to 3 to 5 degrees Celsius to cultivate cold-sea crustaceans. to cultivate.
The culture water drained from the first culture tank is supplied to the second culture tank, and cold-water seaweed is cultured at a water temperature of 10 to 12°C.
The breeding water drained from the second aquaculture tank is supplied to the third aquaculture tank, where salmon are cultured at a water temperature of 14 to 16°C.
The breeding water drained from the third culture tank is supplied to the fourth culture tank, and sea cucumbers are cultured at a water temperature of 16 to 18°C.
The taken deep ocean water is sequentially moved and supplied to the first to fourth aquaculture tanks, and the aquaculture tanks are provided so that the deep ocean water can be moved depending on the water level difference, and a single potential energy is used to move the deep ocean water to the aquaculture tanks. can supply and transport deep ocean water to

The circulation filtration system of the third aquaculture tank is provided with one or more breeding aquariums in which the breeding water drained from the second aquaculture tank is supplied and salmon are cultured, and the breeding water drained from the second aquaculture tank is supplied with the breeding water drained from the second culture tank. After being stored in a settling tank, some of the water is re-supplied to the rearing tank through an organic matter removal device, a deaeration device, a filtration device, and an ozone injection device in order, and the rest is fed to the fourth aquaculture tank as rearing water. It is desirable to be able to supply it as

The organic matter separated from the organic matter removal device is either discharged to the outside or supplied as sea cucumber feed in the fourth aquaculture tank, and carbon dioxide is separated from the rearing water by a degassing device and separated from the rearing water by a filtration device. The nitrogen compounds produced are supplied as nutrients to the seaweed in the second culture tank.

The ozone injection device is composed of an ozone generator that generates ozone and dissolves it in the rearing water, and an ozone control device that performs base monitoring of total residual oxides (TRO), and ozone is added to the rearing water until the TRO concentration is 0. It is desirable to maintain the amount at .040 (mg/L).

The aquaculture method according to the present invention that utilizes the low temperature nature of deep ocean water requires a small difference in water temperature between the pre- and post-aquaculture stages of the different species to be cultured, requires less energy for heating, and prevents the production of marine products due to mistakes in maintaining water temperature. Death can be prevented.
Furthermore, the present invention makes it possible to maintain a low-temperature rearing environment using deep ocean water, making it possible to produce target organisms such as cold-sea aquatic resource salmon, seaweed, and sea cucumbers throughout the year.

Schematic diagram of the multi-step cultivation method of different species using deep ocean water according to the present invention Schematic diagram of the multi-stage cultivation method of different species using deep ocean water of the present invention Schematic diagram of the third culture tank circulation filtration system of the present invention Schematic diagram of the organic matter removal device, deaerator, and filtration device of the present invention Conceptual diagram of the organic matter removal device of the present invention Schematic diagram of the deaerator of the present invention Diagram showing changes in carbon dioxide according to Experimental Example 1 of the present invention A diagram showing the carbon dioxide removal rate by operating the deaerator according to Experimental Example 2 of the present invention. Diagram showing changes in carbon dioxide concentration in the breeding tank during operation of the deaerator according to Experimental Example 2 Diagram showing feed intake of rearing organisms according to TRO concentration according to Experimental Example 3 of the present invention Diagram showing microbial changes depending on TRO concentration according to Experimental Example 3 of the present invention

The multi-step culturing method of different species using deep ocean water according to the present invention will be explained with reference to the drawings.
FIG. 1 shows a schematic diagram of a multi-step cultivation method of different species using deep ocean water according to the present invention, and FIG. 2 shows a schematic diagram of a multi-stage cultivation method of different species using deep ocean water of the present invention. .
The multi-stage cultivation method of different species using deep ocean water of the present invention includes a deep ocean water intake stage (A) of taking seawater from an aqueous layer in which deep ocean water is located, and a first cultivation stage in which the taken deep ocean water is used for aquaculture. The first aquaculture tank supply step (B) in which cold-sea crustaceans are cultured at a water temperature of 3 to 5 degrees Celsius, and the drained aquaculture water that was used as culture water in the step (B) is used in the second aquaculture. A second aquaculture tank supply step (C) in which cold-water seaweed is cultivated at a water temperature of 10 to 12°C, and the breeding water drained in the step (C) is supplied to a third aquaculture tank, and the water temperature is 14 to 12°C. A third aquaculture tank supply step (D) for cultivating salmon at 16°C; a fourth step for cultivating sea cucumbers at a water temperature of 16 to 18°C by supplying the breeding water drained in the step (D) to a fourth aquaculture tank; It consists of the aquaculture tank supply stage (E).

In the present invention, the deep ocean water can be used as aquaculture water by sequentially moving through the first to fourth aquaculture tanks, and the flow of the aquaculture water in the aquaculture tanks can be adjusted by adjusting the water level difference.

The deep sea water intake step (A) of the present invention is a step of taking deep sea water using a water intake pipe.
The deep sea water of the present invention is seawater that is located at a depth of 200 m or less and is not exposed to sunlight, and has excellent cleanliness, low water temperature, and water quality stability at a water temperature of 2°C or less, and has a low amount of dissolved oxygen and contains minerals and nutrients. It is rich in seawater.
Further, in the method of taking deep ocean water according to the present invention, water is taken by extending a water intake pipe to the seabed. As the water intake method, a conventionally known method can be selected.

The first aquaculture tank supply step (B) of the present invention is a step in which the deep ocean water taken in the step (A) is supplied to the first aquaculture tank for aquaculture.
The aquatic organisms cultured in stage (B) are cold-sea crustaceans that can be cultured at a water temperature of 3 to 5°C. In an embodiment of the invention, crab farming was carried out.
The deep ocean water taken in the present invention has the characteristics of low temperature, cleanliness, and no bacteria or viruses, and can be supplied as breeding water without separate heating and water quality control.

The second aquaculture tank supply step (C) of the present invention is a step in which the drained aquaculture water used as the aquaculture water in the step (B) is supplied to the second aquaculture tank for aquaculture.
The aquatic organisms cultivated in step (C) are cold-sea seaweeds that can be cultivated at a water temperature of 10 to 12°C. Seaweeds can include kelp, wakame, and the like.
In the example of the present invention, kelp was cultured in the second culture tank. Young leaves of kelp emerge from December to March and grow until July.It dies when the water temperature exceeds 12 to 13 degrees Celsius, and grows actively in early fall below 10 degrees Celsius, making it possible to cultivate seaweed all year round. .

The third aquaculture tank supply step (D) of the present invention is a step in which the aquaculture water drained in the step (C) is supplied to the third aquaculture tank for aquaculture.
The aquatic organisms cultured in step (D) are preferably salmon that are cultured at a water temperature of 14 to 16°C.
The third aquaculture tank and the fourth aquaculture tank of the present invention preferably consist of a circulating filtration aquaculture system.

FIG. 3 shows a schematic diagram of the circulation filtration aquaculture system of the third aquaculture tank and the fourth aquaculture tank of the present invention.
The circulating filtration aquaculture system of the present invention is provided with one or more breeding aquariums 100 for breeding salmon and sea cucumbers, and is supplied with breeding water drained from the second aquaculture tank or the third aquaculture tank.
The breeding water drained from the breeding tank is stored in a settling tank 200, and then sequentially passes through an organic matter removal device 300, a deaeration device 400, a filtration device 500, and an ozone injection device 600, and some of it is resupplied as water. The water is re-supplied to the third aquaculture tank, and the remainder can be supplied as breeding water to the fourth aquaculture tank.

Circulating filtration aquaculture systems use a power source, such as a submersible pump, to move the aquaculture water through a purification process. Furthermore, by moving the breeding water within the system according to the difference in water level, circulation can be achieved by using power once.
The circulating filtration aquaculture system (RAS) of the present invention is capable of high-density aquaculture, and by increasing productivity and reusing the aquaculture water required for aquaculture, it can be operated with a small amount of water, resulting in huge production capacity. It is possible to reduce power consumption for water pumping.
The breeding tank 100 is a place where salmon are kept. The sedimentation tank 200 temporarily removes solid matter from the breeding water drained from the breeding aquarium.

FIG. 4 shows a schematic diagram of an organic matter removal device, a deaerator, and a filtration device of the present invention. FIG. 5 shows a conceptual diagram of the organic matter removal device of the present invention.
The organic matter removal device 300 of the present invention includes a treatment tank 1 equipped with a lid that is attached to the upper part so as to be openable and closable, and a structure in the middle of the treatment tank 1 that divides the treatment tank into an upper treatment tank 3 and a lower treatment tank 4. An inclined partition part 10 is installed which extends at an angle.

The upper processing tank 3 is provided with a horizontal porous partition 20 that extends horizontally so as to divide the internal space into two parts. A bioball layer 30 in which a plurality of bioballs 31 are stacked is formed between the horizontal porous partition 20 and the inclined partition 10. The bio-ball 31 functions as a degassing filtration medium.

The water supply line is connected to the sedimentation tank 200 to transfer breeding water, and includes a first water supply line and a second water supply line. A first downstream end is connected to the wall of the upper treatment tank 3 higher than the horizontal porous partition 20 , and the first downstream end is connected to the first water supply line 42 . The lower treatment tank 4 is connected to a second water supply line.

The aeration pipe 60 is provided with a venturi pipe 50 whose downstream end connected to the settling tank is connected to the discharge side and whose negative pressure chamber communicates with the atmosphere. The upstream end is connected to the lower end wall of the lower processing tank 4 and communicates with the lower processing tank 4 . The upstream end extends vertically to a position facing the lower part of the inclined partition part 10, and drains the culture water stored in the lower treatment tank 4 according to the water level difference according to the water level of the culture water stored in the lower treatment tank 4. A line 70 can feed the deaerator.

The air supply line 80 is connected to an air supply source 81 and to the wall of the upper treatment tank 3 facing the top of the bioball layer 30 located at the bottom of the horizontal porous partition 20 .
A flow pipe 11 is provided at the lower part of the inclined partition part 10 to allow breeding water to flow between the upper treatment tank 3 and the lower treatment tank 4. A vent pipe 12 is formed in the lower part of the inclined partition part 10, through which air that has flowed into the upper processing tank 3 via the air supply line 80 is drained out of the processing tank via the lower processing tank 4. Ru.

The upstream end inlet of the ventilation pipe 12 is disposed adjacent to the upper surface of the sloped partition 10 in the upper processing tank 3, and is opened downward from the length direction of the sloped partition 10.
The outlet at the downstream end of the ventilation pipe 12 is disposed in the lower treatment tank 4, and is directed toward the upper surface of the organic matter layer floating adjacent to the upper and lower surfaces of the inclined partition portion 10 in the lower treatment tank 4 and the organic matter discharge port 90. It is opened. The organic matter discharged from the discharge port 90 can be used as food for sea cucumbers reared in a fourth aquaculture tank, which will be described later.

The pump 41 is operated while supplying air via the air supply line 80. The breeding water from which heavy organic matter has been filtered from the settling tank 200 is passed through the first water supply line 42, the second water supply line 43, and the aeration pipe 60 to the upper treatment tank 3 and the lower treatment tank 4. supply to

The culture water transferred through the aeration pipe 60 is supplied with air from the lower treatment tank 4 by having atmospheric air flowed in through a Venturi pipe interposed in the middle of the aeration pipe 60 .
Due to the fine air particles generated in the breeding water conveyed through the ventilation pipe and the surface active reaction of the fine air particles, organic residues attached to the surface of the air particles are collected in the form of bubbles at the organic matter outlet 90.

When the organic matter exceeds a certain volume, it can be discharged to the outside and discarded, or it can be supplied to the food of the sea cucumbers reared in the fourth aquaculture tank as described above. At this time, the air that is forcibly discharged and vented to the upper treatment tank 3 through the organic matter discharge port 90 (the air discharged after carbon dioxide removal) is absorbed by the bubbles (microporous particles) collected on the water surface of the lower treatment tank 4. It can act to push out organic matter residues (in the form of organic matter).

The breeding water supplied to the lower treatment tank 4 reaches the middle part of the inclined partition part 10. When the water exceeds the intermediate water level of the inclined partition section 10, the water will overflow through the drainage line and move to the deaerator due to the water level difference.
Organic matter, which is a floating substance contained in the rearing water stored in the lower treatment tank 4, floats on the water surface and is collected near the organic matter discharge port 90.
The breeding water supplied to the upper treatment tank 3 falls onto the horizontal porous partition 20. The fallen breeding water is laminated as a shallow layer on the horizontal porous partition 20, passes through the plurality of through holes formed in the horizontal porous partition 20, and falls onto the bioball layer.

The air that has passed through the air supply line cannot pass through the horizontal porous partition 20 through the plurality of through holes in the horizontal porous partition 20 due to the culture water layered in a shallow layer on the horizontal porous partition 20, and the bio After penetrating the ball layer 30 and flowing downward, it is discharged to the outside of the processing tank via the ventilation port and the organic matter discharge port 90.

The breeding water that has fallen onto the bio-ball layer 30 permeates through the bio-ball layer 30, increasing the contact area and contact time with the air that is permeating through the bio-ball layer 30, maximizing the reaction efficiency with the air. . Carbon dioxide, which is highly dissolved in the rearing water, is removed by being blown into the air above the water surface as the water surface is strongly agitated by the infiltrating air.

The breeding water from which dissolved carbon dioxide has been removed cannot flow into the lower treatment tank 4 through the vent due to gravity, but instead flows into the lower treatment tank 4 through the flow pipe 11. Organic matter in the breeding water supplied to the lower treatment tank 4 through the flow pipe, the second water supply line, and the aeration pipe floats to the surface and is collected adjacent to the upper and lower surfaces of the inclined partitions 10 in the lower treatment tank 4.
The collected organic matter is forced toward the organic matter outlet 90 by air released through the vent pipe 12 and discharged from the treatment tank.

The organic matter removal device according to the present invention uses a single power source for a single treatment tank in order to remove dissolved carbon dioxide and organic matter, thereby reducing manufacturing and operating costs for culture water treatment compared to conventional methods. The separated organic substances can be supplied as feed to cultured organisms (sea cucumbers) in a fourth culture tank, which will be described later.

FIG. 6 shows a schematic diagram of the degassing device of the invention.
The deaerator of the present invention is provided with a packed column 410 having a certain size and an empty interior.
Filtration media 440 are stacked inside the packed column 410 . The deaerator according to the present invention can be operated at an air flow rate of 25 m ³ /min to 50 m ³ /min.

A rearing water inflow pipe 420 is provided at the upper part of the packed tower 410, which is connected to an organic matter removal device and allows the rearing water from which organic matter has been removed to flow into the inside. The breeding water inflow pipe 420 is provided with an inflow amount adjustment valve 430 that can adjust the amount of inflow of the breeding water. The inflow rate regulating valve 430 regulates the flow rate of breeding water flowing in from the organic matter removal device. By adjusting the volume of breeding water flowing into the filtration medium 440, the deaeration effect can be maximized by supplying an appropriate flow rate.

A discharge pipe 450 is provided at the bottom of the packed tower 410 so that the breeding water that has passed through the filtration medium 440 can be transferred to the filtration device. An air inlet 460 for supplying air into the inside of the packed tower 410 and an air outlet 470 for discharging the air supplied through the air inlet 460 to the outside of the packed tower are provided on either side of the packed tower 410. It is provided.
Furthermore, an air adjustment valve 480 that can adjust the amount of air injected and discharged from the air inlet 460 and the air outlet 470 may be provided.

The breeding water inflow pipe 420 is an inlet pipe that supplies high-concentration dissolved carbon dioxide generated by respiration of the cultured organisms raised in the third culture tank to the deaerator. The air adjustment valve 480 is a valve that adjusts the amount of breeding water supplied to the deaerator, and can supply an appropriate flow rate that matches the capacity of the device to maximize the effect.

It is preferable that the discharge pipe 450 is formed in the form of an S-TRAP so that air does not pass therethrough as an outlet for the culture water after the deaeration stage has been completed. The packed tower cover part is sealed to prevent air ventilation. A perforated plate in the form of water sprinkling is installed at the lower end of the sealed lid to evenly distribute breeding water to the filtration medium 440 stored in the packed tower.

A blower provided at the air outlet 470 is used to ensure that the air to be taken in and exhausted is supplied into the device only through the air inlet 460. At this time, the air regulating valve 480 can regulate the intake amount of air and the amount of air supplied to the interior, in order to regulate the intensity of the negative pressure inside.

It is preferable that the air outlet 470 is installed outside the packed tower 410 at a place where the air sucked into the device is discharged. According to embodiments of the invention, ventilators and blowers for forced suction and evacuation, etc. were implemented.
Although the air outlet 470 and the air inlet 460 can be installed regardless of the number, the total cross-sectional area of the air inlet 460 must be smaller than the total cross-sectional area of the air outlet 470. Additionally, the air outlet 470 and the air inlet 460 are placed opposite to increase air flow efficiency.

Furthermore, by providing an air control valve 480 on either side of the packed tower 410, it is possible to easily and precisely adjust the amount of air injection to adjust the pressure intensity within the packed tower. As a result, a fine negative pressure is formed inside the packed column of the present invention, and the carbon dioxide (CO ₂ ) evacuation capacity can be maximized.

The inside of the packed tower 410 where the air inlet 460 and the air outlet 470 are provided has a structure in which a medium is installed and the air is sealed, and breeding water is injected into the medium. Once the carbon dioxide is separated from the injected rearing water, the air outlet 470 will quickly suck in and exhaust the carbon dioxide, improving the deaeration efficiency compared to existing and conventional deaeration devices.

The filtration media 440 is stored inside the packed column 410, and is installed by vertically stacking plastic material platens maintaining regular spacing in a grid pattern. Deaeration occurs when the breeding water falls vertically and hits the filter medium 440 to be crushed into particles. The culture water flowing into the upper part of the filter medium passes through the filter medium, and the culture water particles collide and are minimized. At this time, carbon dioxide (CO ² ) is separated from the culture water particles, and since the culture water molecules are relatively larger than air molecules, they flow downward through the holes formed in the medium.

The deaerator of the present invention can fill the medium in the column in the form of a packaged column, allowing the passing water to effectively contact the air and supply more oxygen.
A breeding water inflow pipe 420 is connected to the upper part of the packed tower. The breeding water flowing into the breeding water inflow pipe 420 contains highly concentrated dissolved carbon dioxide generated by the respiration of the cultured organisms from which organic matter has been removed.
The culture water discharge pipe 420 has an S-trap structure, so that air is not injected into the packed tower and only the collected culture water is discharged to the outside. Carbon dioxide is removed through the medium, and the culture water collected in the lower part can be transferred to a subsequent filtration device.

Preferably, the filtration device according to embodiments of the invention is a fluidized bed filtration device using biball filtration media. Dissolved substances such as ammonia and nitrite remaining in the rearing water can be purified through the filtration device of the present invention, and the water can be reused as rearing water. In this case, the separated underwater ammonia and nitrite can be provided as nutrients to the seaweed in the second culture device.

The breeding water that has passed through the filtration device is supplied to an ozone injection device. Normally, ozone is a gaseous substance in which free oxygen atoms combine with oxygen molecules, and due to its instability, it exhibits strong oxidizing power in water. Ozone quickly reacts with dissolved Br- in seawater to produce BrO-/OHBr and has a strong bactericidal effect, which is highly effective in controlling microorganisms in breeding water and removing nitrogen compounds in water.
However, ozone is highly biologically hazardous. In particular, aquaculture organisms react sensitively to ozone-derived residual oxides, and there are many difficulties in applying them to aquaculture methods. ozone injection is required.

Total residual oxides (TRO) are the remaining oxidized materials. Total residual oxides will not cause fish mortality if TRO concentration levels are maintained that are safe for fish.
The process of treating seawater with ozone regarding the production and action of TRO is different from freshwater, and the chemical reaction process is much more complex due to the abundant presence of bromine ions (Br-) in seawater.
The ozone reaction in seawater forms multiple reactions while reacting with some chemical seawater compounds very quickly, so the half-life of ozone is only a few seconds. The oxidizing agents produced by ozone are called OPOs, and the main OPOs are hypoabromate (HOBr) and hypoabromate ion (OBr-). Since OPO is highly toxic to fish depending on its concentration, controlling residual OPO during the circulation stage is most important for the protection of farmed fish.

If the concentration exceeds the appropriate permissible concentration required by fish, it must be neutralized before being fed. It is desirable that the amount of ozone injected into the breeding water in the ozone injection device according to the present invention has a TRO concentration of 0.04 (mg/L). The amount of the TRO concentration can prevent the death of fish when the breeding water is re-supplied to the third breeding tank or drained to the fourth breeding tank and supplied to the breeding water after the breeding water is ozonated. It is concentration.

The ozone injection device consists of an ozone generator that generates ozone and dissolves it in the rearing water, and an ozone control device that performs total residual oxide (TRO) substrate monitoring.
The ozone generator according to the embodiment of the present invention generates ozone by selecting one or more of an asexual discharge method, an electrolytic method, a photochemical reaction, a radiation irradiation method, and a high frequency electric field method.
Further, an ozone dissolver and an oxygen generator can be additionally installed in the ozone generator.

The ozone control device of the present invention can be monitored by continuous measurement of total residual oxide (TRO), and in the embodiment, it is implemented using a commonly known HF scientific CLX Online residual chlorine monitor (U.S.A.). There is. Total residual oxide (TRO) based monitoring system control system has microbial sensitization properties and operational stability.

In the circulating filtration culture system according to the present invention, once certain pathogenic bacteria or parasites are introduced through recirculation of the culture water, they rapidly spread throughout the system. Therefore, the ozone injection device according to the present invention needs to supply ozone with excellent bactericidal effects to kill pathogens and parasites.
The fourth aquaculture tank supply step (E) of the present invention is a step in which the aquaculture water drained in the step (D) is supplied to the fourth aquaculture tank for aquaculture.
The aquatic organisms cultured in step (E) are preferably sea cucumbers cultured at a water temperature of 16 to 18°C. In step (D) above, organic matter including salmon excrement and feed residue discharged from salmon farming can be discharged into the drained rearing water together, and such aquaculture organic matter can be used for sea cucumber cultivation. Can be provided as bait. Further, the fourth aquaculture tank of the present invention can be operated as a circulating filtration aquaculture system using the same method as the third aquaculture tank described above.

The carbon dioxide and nitrogenous compounds separated into the culture water in step (D) or (E) of the present invention can be re-supplied to the second culture tank to supply seaweed nutrients.
The breeding water that is transferred to the above stages (D) to (E) is temporarily collected in a water tank without being heated before being transferred to the later stages, and the water temperature is raised at room temperature, or the water is heated. The water can be heated by a device and supplied to each aquarium at a set temperature.

Normally, land-based aquaculture farms require a large amount of thermal energy supply system to maintain a constant temperature due to seasonal changes, and accidents continue to occur in which large numbers of organisms die due to mistakes in maintaining the appropriate temperature. Additionally, supplying minerals using deep ocean water causes the water temperature in the winter aquaculture tank to drop rapidly, requiring sophisticated temperature correction.
However, in the aquaculture method according to the present invention, since the water temperature difference required between the front and rear aquaculture stages is not large, less energy is required for heating, and death of aquatic organisms due to mistakes in maintaining water temperature can be prevented.

In addition, target organisms such as salmon, seaweed, and sea cucumbers, which are cold-sea aquatic resources cultivated according to the present invention, can be produced year-round if it is possible to maintain a low-water temperature rearing environment using deep water. Because of its advantages, it has the effect of increasing the production rate of aquaculture organisms.
The aquaculture method using each of the above steps allows for separation and combination, so it is effective in cultivating only the fish species to be produced as needed.

The following experimental example confirms the effects of operating the deaerator and ozone injection device in the third aquaculture tank.
<Experiment example 1> Confirmation of carbon dioxide concentration in the deaerator In experiment example 1, after installing the deaerator in the circulation filtration system of the third aquaculture tank that is normally operating, we distinguish between the non-operating state and the normal operating state. Carbon dioxide was measured.
Feed was normally fed for 5 days, and after stopping feed supply, the speed at which carbon dioxide reached the highest and lowest points was measured, and the carbon dioxide concentration in the rearing water was determined depending on whether the deaerator was in operation or not. The reduction efficiency was compared. The carbon dioxide concentration in the rearing water was measured at 10 minute intervals, starting from the last day of feeding until the lowest concentration point.

FIG. 7 is a diagram showing changes in carbon dioxide according to Experimental Example 1 of the present invention.
When the deaerator was not operated, it started at 19.4 mg/L and rose to a maximum of 29.2 mg/L. It took about 29.5 hours for carbon dioxide to reach the lowest point of 11.4 mg/L.
On the other hand, when the deaerator was started, the concentration started at about 16.1 mg/L, rose to a maximum of 24.7 mg/L, and required about 24.9 hr to reach the lowest point of 11.3 mg/L.
When the deaerator was not in operation, the concentration of carbon dioxide was 1.18 times higher than the maximum concentration standard than when it was in operation, indicating that it could be improved by the deaerator. It was found that the concentration of carbon dioxide was also kept low during operation.

<Experimental Example 2> Efficiency of the deaerator based on deaerator operating efficiency (air flow rate) Experimental example 2 was carried out in the same manner as Experimental example 1. Table 1 below shows the removal efficiency conditions depending on the amount of air supplied to the deaerator, and FIG. 8 is a diagram showing the carbon dioxide removal rate depending on the operation of the deaerator.
When the deaerator was not operating at an air flow rate of 0 m ³ /min, it showed an unstable and low removal efficiency of 31.8 ± 7.3% on average, but when the air flow rate was 25 m ³ /min, , an average of 46.2±2.9%, and when the air flow rate was 50 m ³ /min, a stable removal efficiency of an average of 59.0±3.1% was shown.

Next, the carbon dioxide concentration of the breeding water was confirmed based on the operating efficiency (airflow amount) of the deaerator.
In order to investigate the maintenance range of dissolved carbon dioxide concentration in the system and the removal efficiency according to the air flow rate of the deaerator in normal operation, the following tests were conducted: 0 m ³ /min (not operating), 25 m ³ /min, and 50 m ³ /min. Comparisons were made taking into account the difference in air flow. The concentration of dissolved carbon dioxide in the rearing water was continuously measured at 10 minute intervals for 5 days while feeding was normally provided (6 water tanks x 5 kg = 30 kg/day).

FIG. 9 is a diagram showing changes in carbon dioxide concentration in the rearing tank during operation of the deaerator according to Experimental Example 2.
When the blower of the deaerator was not operating, the concentration was maintained at a minimum of 25 to 38 mg/L, but when the air flow rate was 25 m ³ /min, it was 20 to 30 mg/L.
When the air flow rate was 50 m ³ /min, the concentration ranged from 18 to 28 mg/L.

During the daytime when feed was supplied in all experimental areas, the concentration of carbon dioxide increased due to the activity of reared fish and increased respiration associated with feed intake, but after sunset when activity and feed intake were interrupted, dissolved carbon dioxide concentration increased. It appeared that the concentration decreased.

<Experimental Example 3> Confirmation of appropriate TRO concentration In Experimental Example 3, the TRO concentration in the rearing tank was measured in real time using the TRO continuous measuring device according to the present invention, and changes in the behavior (feed intake standard) of rearing organisms and changes in the rearing water were investigated. The microbial changes were investigated.
The experimental fish according to Experimental Example 3 of the present invention was sea trout, and the effect of ozone treatment in a high-density rearing system was investigated. Feed intake was investigated by manually feeding the animals to ensure that no remaining feed was left on the basis of full feeding when feed was fed twice a day.
Microbial investigation items include heterotrophic marine bacteria, which are closely related to the filtering microorganisms in biological filtration tanks and may affect filtration efficiency, and gram-negative strains and Vibrio spp, which are pathogenic microorganisms that are significantly involved in fish diseases. We conducted a survey targeting the following.

Table 2 below is a diagram showing changes in feed intake and changes in microorganisms in breeding water depending on TRO concentration. FIGS. 10 and 11 show feed intake of rearing organisms (FIG. 10) and microbial changes (FIG. 11) according to TRO concentration according to Experimental Example 3 of the present invention.
The feed intake of the reared organisms was 2,168 g/day in the TRO 0.00 experimental area (control area), 2,155 g/day in the TRO 0.04 experimental area, and 713 g/day in the 0.08 experimental area. At a concentration of TRO 0.08 or higher, it is judged that abnormal changes occur in the feed intake of the reared organisms.

Among the breeding water microorganisms, Heterotrophic marine bacteria was 3.4 x 10 ³ CFU/ml in the TRO 0.00 experimental area (control area), and 1.0 x 10 ³ CFU/ml in the TRO 0.04 experimental area, 0. It was 1.0×10 ³ CFU/ml in the 08 experimental section.
Gram-negative strain is 1.4 x 10 ³ CFU/ml in the control area, 5.6 x 10 ¹ CFU/ml in the 0.04 experimental area, and 4.5 x 10 ¹ CFU/ml in the 0.08 experimental area. Detected in ml.

In the case of Vibrio spp, the control area was 9.5 x 10 ² CFU/ml, the TRO 0.04 experimental area was 2.3 x 10 ¹ CFU/ml, and the 0.08 experimental area was 0.7 x 10 ¹ CFU/ml. Met.
In the TRO 0.04 mg/L experimental area, the population of Gram-negative strain and Vibrio spp microorganisms, which greatly affect fish diseases, decreased to 1/26 and 1/41 levels, respectively, and in the 0.08 mg/L experimental area, Gram - Negat ive strain was 1/32 and there was no major change, but Vibrio spp was found to be at the 1/127 level, which was about 3 times lower than 0.04.

Increasing the TRO concentration in the rearing water has the advantage of reducing disease-causing microorganisms, but it is considered dangerous to inject ozone at a concentration higher than the one that affects fish growth, such as by reducing feed intake. When rearing sea trout in a circulating filtration aquaculture system, the ozone titration concentration has no effect on feed intake and the number of microorganisms is significantly reduced. We confirmed that it is desirable to drive.

The multi-stage aquaculture method for heterogeneous organisms using deep ocean water according to the present invention combines the advantages of deep ocean water and the advantages of environmentally friendly advanced aquaculture technology, and promotes an environmentally friendly deep ocean water advanced aquaculture business, thereby promoting the future. It is possible to evolve into a type export industry, lead the world market, and have industrial applications.

1: Treatment tank 10: Inclined partition section 11: Distribution pipe 12: Ventilation pipe 20: Horizontal porous partition section 3: Upper processing tank 30: Bio ball layer 31: Bio ball 4: Lower processing tank 40: Venturi tube 41: Pump 42 : First water supply line 43: Second water supply line 60: Aeration pipe 80: Air supply line 81: Air supply source 90: Discharge port 100: Breeding tank 200: Sedimentation tank 300: Organic matter removal device 400: Deaerator 410 : Packed tower 420: Breeding water inflow pipe 430: Inflow control valve 440: Filtration medium 450: Discharge pipe 460: Air inlet 470: Air outlet 480: Air control valve 500: Filtration device 600: Ozone injection device

Claims (2)

The taken deep ocean water is supplied to the first aquaculture tank, and the cold-sea crustaceans are cultivated at a water temperature of 3 to 5°C. The aquaculture water drained from the first aquaculture tank is supplied to the second aquaculture tank, and the water temperature is Cultivate seaweed at 10-12℃,
The breeding water drained from the second culture tank is supplied to the third culture tank, where salmon are cultured at a water temperature of 14 to 16°C, and the culture water drained from the third culture tank is supplied to the fourth culture tank. Then, sea cucumbers are cultivated at a water temperature of 16 to 18 degrees Celsius.
The taken deep ocean water is sequentially supplied to the first to fourth aquaculture tanks using water level transfer energy, and the third and fourth aquaculture tanks are configured with a circulation filtration aquaculture system to recirculate the aquaculture water. ,
A multi-step culture method for heterogeneous organisms using deep ocean water, characterized in that the total residual oxide (TRO) concentration of the culture water is maintained at 0.04 to 0.08 (mg/L) or less . The circulating filtration aquaculture system is provided with one or more breeding tanks, and the breeding water drained from the breeding tank is stored in a sedimentation tank, and then subjected to an organic matter removal device, a deaeration device, a filtration device, and an ozone injection device. They are sequentially re-supplied to the aquarium,
The organic matter separated from the organic matter removal device was either discharged to the outside or supplied as sea cucumber food in the fourth aquaculture tank, and the carbon dioxide was separated from the rearing water using a deaerator and separated from the rearing water using a filtration device. 2. The multi-stage cultivation method for different species using deep ocean water according to claim 1, wherein the nitrogen compound is supplied as a nutrient salt to the seaweed in the second cultivation tank.