JP7445557B2 - Analysis method, analysis device that uses the analysis method, and program - Google Patents

Description

TECHNICAL FIELD This disclosure relates to spectroscopic analysis. More specifically, the present invention relates to an analysis method, apparatus, and program using parameter correction based on back data using a reference wavelength.

In recent years, as health awareness has increased, demand has increased for analytical instruments that can easily monitor health conditions at home. Patent Document 1 discloses a technology in which an infrared analyzer is built into a toilet bowl, a thermal light source suitable for mass production is used as a light source, and the concentration of components in urine is analyzed using a total reflectance measurement (ATR) detection unit. Proposed.

JP2009-204598A

Thermal light sources have the characteristic that the amount of light changes depending on the temperature, so there is a problem that the detection intensity fluctuates and reliability decreases. Furthermore, light sources tend to have a tendency for the amount of light to decrease depending on the usage time due to deterioration or the like.

The purpose of the present disclosure is to provide an analysis method that can reduce the influence of variations in detection intensity due to variations in light intensity on analysis results and provide highly reliable data, an analysis device that employs the spectroscopic analysis method, and an analysis method that can provide highly reliable data. The purpose is to provide a program that implements the method.

The analysis method according to the present disclosure is an analysis method for identifying the concentration of a component to be inspected by spectrum analysis of a light beam transmitted through an optical element,
(a) In the first measurement environment parameter, the intensity at a first wavelength of the spectrum of the sample containing the test component at the first concentration and the intensity at a second wavelength different from the first wavelength are determined. acquiring and storing in association with the first measurement environment parameter;
(b) In a second measurement environment parameter different from the first measurement environment parameter, the intensity at the first wavelength and the second wavelength of the spectrum of the sample containing the component to be tested at the first concentration are determined. and storing the intensity in association with the second measurement environment parameter;
(c) determining the ratio of the intensity at the first wavelength in the first measurement environment parameter and the intensity at the first wavelength in the second measurement environment parameter; The process of linking and saving,
(d) in the first measurement environment parameter, obtain the intensity at the first wavelength of the spectrum of the sample containing the test component at a second concentration different from the first concentration; a step of storing in association with the second concentration;
(e) obtaining intensities at the first wavelength and the second wavelength for a spectrum of the test object containing the test component at an arbitrary concentration in an arbitrary measurement environment parameter;
(f) The intensity at the second wavelength obtained in the step (e) is linked and stored in the step (b) with the second measurement environment parameter. determining the arbitrary measurement environment parameter by comparing it with the
(g) The arbitrary measurement environment parameter determined in the step (f) was replaced with the second measurement environment parameter, and the result was stored in association with the second measurement environment parameter in the step (c). Multiplying the intensity of the first wavelength obtained in step (e) by the ratio and replacing it with the intensity of the first wavelength in the first measurement environment parameter;
(h) Compare the intensity of the first wavelength obtained by replacing in the step (g) with the intensity at the first wavelength stored in association with the second concentration in the step (d). determining the arbitrary concentration by
Equipped with.

Further, a program according to the present disclosure is a program for executing an analysis to identify the concentration of a component to be inspected by spectrum analysis of a light beam transmitted through an optical element,
(a) In the first measurement environment parameter, the intensity at a first wavelength of the spectrum of the sample containing the test component at the first concentration and the intensity at a second wavelength different from the first wavelength are determined. acquiring and storing in association with the first measurement environment parameter;
(b) In a second measurement environment parameter different from the first measurement environment parameter, the intensity at the first wavelength and the second wavelength of the spectrum of the sample containing the component to be tested at the first concentration are determined. and storing the intensity in association with the second measurement environment parameter;
(c) determining the ratio of the intensity at the first wavelength in the first measurement environment parameter and the intensity at the first wavelength in the second measurement environment parameter; The process of linking and saving,
(d) in the first measurement environment parameter, obtain the intensity at the first wavelength of the spectrum of the sample containing the test component at a second concentration different from the first concentration; a step of storing in association with the second concentration;
(e) obtaining intensities at the first wavelength and the second wavelength for a spectrum of the test object containing the test component at an arbitrary concentration in an arbitrary measurement environment parameter;
(f) The intensity at the second wavelength obtained in the step (e) is linked and stored in the step (b) with the second measurement environment parameter. determining the arbitrary measurement environment parameter by comparing it with the
(g) The arbitrary measurement environment parameter determined in the step (f) was replaced with the second measurement environment parameter, and the result was stored in association with the second measurement environment parameter in the step (c). Multiplying the intensity of the first wavelength obtained in step (e) by the ratio to replace the intensity of the first wavelength in the first measurement environment parameter;
(h) Compare the intensity of the first wavelength obtained by replacing in the step (g) with the intensity at the first wavelength stored in association with the second concentration in the step (d). determining the arbitrary concentration by
It is used to make a computer execute.

FIG. 1 is a schematic diagram showing an example of a measuring device that employs a correction method according to the present disclosure. FIG. 2 is an enlarged cross-sectional view of a portion of the device according to FIG. 1 surrounded by a dotted line A; FIG. 2 is a plan view showing the relationship between the optical path and the test sample in the apparatus according to FIG. 1. FIG. FIG. 2 is a plan view illustrating an optical path in the device according to FIG. 1. FIG. FIG. 2 is a block diagram showing an example of the functional configuration of an analysis device. It is a flow chart showing an example of determining the salt concentration in urine using the analysis method according to the present disclosure. FIG. 3 is a diagram illustrating a correction method according to the present disclosure. FIG. 2 is a diagram illustrating a correction method according to the present disclosure. FIG. 2 is a diagram illustrating a correction method according to the present disclosure.

In the following embodiments, the same components are denoted by the same reference numerals, and redundant explanations will be omitted. Further, in each drawing, for convenience of explanation, some of the constituent elements may be omitted as appropriate, and the dimensions of the constituent elements may be enlarged or reduced as appropriate.

The analysis method according to the present disclosure will be explained below using an example applied to spectral analysis to examine the salt concentration in a urine sample during urination. There are no restrictions on the test component or test component as long as the test component can be analyzed.

FIG. 1 is a schematic diagram showing an example of a measuring device that employs the analysis method according to the present disclosure. FIG. 2 is an enlarged sectional view of a portion of the device according to FIG. 1 surrounded by a dotted line A. 3 is a plan view showing the relationship between the optical path and the test sample in the apparatus according to FIG. 1, and FIG. 4 is a plan view illustrating the optical path in the apparatus according to FIG. 1.

In this embodiment, an analysis device that employs the light amount correction method according to the present disclosure is configured as a toilet bowl urine analysis device that analyzes light that passes through an ATR optical element.

In FIG. 1, the toilet bowl urine component analyzer 26 (hereinafter sometimes simply referred to as the analyzer 26) is housed in the functional section 14 provided at the rear upper part of the toilet bowl 12 when not in use, and when in use. It is attached to the tip of a cylindrical member 34 that can extend into the toilet bowl 22. In this embodiment, the cylindrical member 34 is a telescoping type consisting of an outer cylinder and an inner cylinder, and is housed in the functional part 14 when not in use, and is automatically removed from the functional part 14 when in use. Or it advances into the toilet bowl 22 manually (operator's switch operation, etc.). Note that the analyzer 26 may be stored in any manner; for example, the analyzer 26 may be stored with its arms in a toilet seat, then slid sideways to bring the tip above the water seal 24.

When the analyzer 26 that has advanced into the toilet bowl 22 comes into contact with the urine urinated into the toilet bowl 22, it becomes possible to identify the urine component to be tested, analyze its concentration, etc. using the function of the optical system, which will be described later. . By extending the cylindrical member into the toilet bowl 22 only during urination, it is possible to analyze urine components in a natural state without making the subject feel pressured or nervous. Moreover, since excess urine is discharged via the water seal 24 and the drain pipe 36, post-treatment is easy.

FIG. 2 is a partially enlarged view of the dotted line area A in FIG. 1, showing a partial cross section of the analyzer 26. The analyzer 26 in this embodiment is a device that identifies components in urine, measures concentrations, etc. by spectrum analysis using attenuated total reflection method (ATR method), which is one of the spectroscopic analysis methods.

The analysis device 26 according to this embodiment will be described below with reference to FIG. 2. The analysis device 26 includes a light source 38 that emits a light beam for analysis, a prism P that functions as an ATR element that propagates the light beam from the light source, and a photosensor 40 that receives the light beam emitted from the prism P. The light source 38, prism P, and optical sensor 40 are arranged near the tip of the cylindrical member 34.

Prism P has a urine contact surface 26c that contacts urine Ur. When urine Ur comes into contact with the urine contacting surface 26c of the prism P, the light beam emitted from the light source 38 enters the entrance surface 26a of the prism P, repeats total reflection between the upper and lower surfaces of the prism, and is reflected inside the prism P. The light travels through the prism P and exits from the exit surface 26b of the prism P.

The light beam emitted from the output surface 26b of the prism P enters the lens 42 of the optical sensor 40. The light beam incident on the optical sensor 40 is sent to a data processing unit by wire or wirelessly and analyzed. The data processing section may be provided around the toilet bowl or may be provided at a remote location.

By providing the lens 42 with a filter function that transmits the infrared absorption wavelength of the component to be detected, it is possible to easily obtain the waveform to be analyzed. A filter separate from the lens 42 may be provided as long as an equivalent function can be obtained. According to the method of the present disclosure, even when there are a plurality of components to be detected, it is possible to obtain data that has undergone light intensity correction using one reference wavelength, which is advantageous in terms of miniaturization and low cost.

3 is a plan view showing the relationship between the optical path and the test sample in the apparatus according to FIG. 1, and FIG. 4 is a plan view illustrating the optical path in the apparatus according to FIG. 1. As illustrated in FIG. 3, during actual urine measurement, the urine Ur that is the subject comes into contact with the upper surface (referred to as the urine contact surface 26c) of the analyzer 26 installed at the tip of the cylindrical member 34. At this time, it is preferable that urine Ur be spread over the entire optical path Po.

As illustrated in FIG. 4, in a state where the urine Ur is in contact with the optical path Po, the light beam from the light source 38 (the corresponding position is indicated by a dotted line in the figure, see also FIG. 2) enters the entrance surface 26e of the prism P. The light enters the prism P, travels toward the exit surface 26b while being totally reflected inside the prism P, exits from the exit surface 26f of the prism P, and enters the optical sensor 40 (the corresponding position is indicated by a dotted line in the figure, see also FIG. 2). do. Any arbitrary sensor can be used as the optical sensor 40, and for example, a pyroelectric sensor or the like can be used. In order to advance the light beam within the prism P by total internal reflection, the refractive index of the prism P must be greater than the refractive index of the object.

FIG. 5 is a block diagram showing an example of the functional configuration of the analysis device 26. As shown in FIG. The analysis device 26 includes a sensor unit 28, a control section 30, and a data processing section 32. The control unit 30 and the data processing unit 32 are realized by a combination of hardware elements and software elements, or only by hardware elements. As the hardware elements, a processor, ROM (Read Only Memory), and RAM (Random Access Memory) are used. Programs such as operating systems and applications are used as software elements.

The hardware elements constituting the control section 30 and the data processing section 32 may be housed inside the functional section 14 or may be provided around the functional section 14 or at a remote location. The control section 30 controls the operation of the sensor unit 28. The data processing section 32 performs spectrum analysis based on the detection signal output from the optical sensor 40 of the sensor unit 28.

Hereinafter, a method for accurately determining the salt concentration contained in urine using the analyzer having the above configuration will be described. The following explanation is intended to deepen understanding of the technology related to the present disclosure, and is not intended to limit the scope of the claims related to the present disclosure.

FIG. 6 is a flowchart illustrating an example of determining the salt concentration in urine using the analysis method according to the present disclosure. 7 to 9 are conceptual diagrams complementary to the flowchart of FIG. 6.

In the following explanation, the test target is urine, the test component is salt (NaCl), the temperature _T0 corresponds to the first measurement environment parameter, the temperature Tx corresponds to the second measurement environment parameter, and the test target is salt (NaCl). The peak wavelength of the spectrum of component NaCl corresponds to the first wavelength, the reference wavelength corresponds to the second wavelength, and the coefficient α is the intensity at the first wavelength in the first measurement environment parameter (hereinafter, the It corresponds to the ratio of the intensity at the first wavelength (peak wavelength) (sometimes referred to as peak intensity) and the peak intensity at the second measurement environment parameter, and the intensities Iy ₁ [NaCl], Iy ₂ [NaCl], Iy ₃ [NaCl] and Iy ₄ [NaCl] correspond to the peak intensities at a plurality of second concentrations Cy=C ₁ , C ₂ , C ₃ , and C ₄ of NaCl in the test object, respectively.

In the analysis method according to the present disclosure, attention is paid to temperature as an index of light intensity fluctuation, and first, the peak intensity I ₀ [NaCl] of the spectrum of the sample containing NaCl at temperature T ₀ and the intensity I ₀ [ref] at the reference wavelength (hereinafter referred to as , the intensity at the reference wavelength is sometimes referred to as reference intensity) and is stored as back data (S11). The intensity I ₀ [NaCl] corresponds to the intensity at the peak wavelength, and the intensity I ₀ [ref] corresponds to the intensity at the reference wavelength. The reference wavelength may be a wavelength different from the peak wavelength of the component to be inspected, and may be a wavelength that does not affect the intensity of the spectrum depending on the concentration of the component to be inspected in the test object. FIG. 7 is a graph of the obtained spectrum, with the horizontal axis representing the wavelength λ and the vertical axis representing the intensity I. It is preferable that the temperature T ₀ is set to the temperature at which the light intensity of the light source 38 is the largest at the time of product warranty (at the start of use) (for example, the state where the light intensity of the light source 38 is set to the maximum at the time of product warranty). According to the method according to the present disclosure, the temperature T ₀ serves as a guideline for light amount correction. Although it is possible to upwardly correct the intensity of the spectrum when the light intensity of the light source 38 decreases, if the light intensity of the light source 38 increases more than the light intensity of the light source 38 at the temperature _T0 set as the reference value, the intensity of the spectrum This is because it is not possible to correct downward. The reference wavelength may be any wavelength as long as the intensity does not change depending on the concentration of the component to be tested (for example, a wavelength outside the absorption wavelength range of each component to be tested).

Next, the peak intensity Ix[NaCl] and the reference intensity Ix[ref] at a plurality of temperatures Tx are obtained, and are stored as backtaters in association with each temperature Tx (S12). In the example of FIG. 8, the peak intensities Ix[NaCl]=Ix ₁ [NaCl], Ix ₂ [NaCl] and Ix ₃ [NaCl] at multiple temperatures Tx=T ₁ , T ₂ and T ₃ are determined, respectively. . As illustrated in FIG. 7, the temperature Tx is set at at least two points, preferably three or more points, within a range where temperature fluctuations of the thermal light source 38 are expected, and the peak intensity Ix [NaCl] at each Tx is determined. It is preferable. However, the present invention is not limited to this, and the temperature Tx may be set at only one point within a range in which temperature fluctuations of the thermal light source 38 are expected.

Next, the value of the coefficient α for each Tx is determined from equation (1) and is stored as back data in association with the Tx (S13).
α×Ix[NaCl]=I ₀ [NaCl] (1)

Here, the following relationship holds between the intensity of the light beam that enters and passes through the prism P, the extinction coefficient of the specimen, the concentration of the specimen, and the optical path length of the prism P.
log ₁₀ (I _in /I _o ut)=εcd (2)

In the above equation, I _in is the intensity of the incident light that enters the prism P, I _out is the intensity of the outgoing light that passes through the prism P, ε is the molar extinction coefficient of the component to be tested in the specimen, and c is the to-be-tested component in the specimen. The molar concentration of the component, d, is the optical path length, and the right side εcd is the absorbance.

From the above equation, the following equations (3) and (4) are derived.
I _in /I _out =10εcd (3)
I _out = I _in /10εcd (4)
From the above equation (4), the intensity I _out of the outgoing light that passes through the prism P is determined by the molar extinction coefficient ε of the component to be tested in the specimen and the intensity I _in of the incident light that enters the prism P. It can be seen that it is determined by the molar concentration c and the optical path length d. In this embodiment, since the molar extinction coefficient ε is specific to the substance and the optical path length d is constant, I _out is determined by the molar concentration c. The present disclosure is inspired by the principle that the molar concentration of a test component in a specimen can be calculated backwards by measuring the transmitted light intensity I _out .

Next, the peak intensities Iy[NaCl] at a plurality of concentrations Cy are determined for the peak wavelength λ[NaCl] at the temperature _T0 , and are stored as back data in association with each concentration Cy (S14). FIG. 8 is a graph in which the horizontal axis is the wavelength λ and the vertical axis is the intensity I for the obtained spectrum. In S14, peak intensities Iy at a plurality of concentrations Cy=C ₁ , C ₂ , C ₃ , C ₄ are determined using spectra of samples with concentrations Cy=C ₁ , C ₂ , C ₃ , and C ₄ of the components to be tested. [NaCl]=Iy ₁ [NaCl], Iy ₂ [NaCl], Iy ₃ [NaCl] and Iy ₄ [NaCl] are determined (see FIG. 8). As illustrated in FIG. 8, the accuracy can be expected to improve as the number of data increases, but it is preferable to obtain the NaCl peak intensity Iy[NaCl] at the concentration of at least two points, preferably three or more points. However, the present invention is not limited to this, and the peak intensity Iy[NaCl] may be determined based on the concentration at only one point. Note that since the intensity at the reference wavelength λ[ref] does not depend on the concentration, _I0 [ref]=Iy[ref] is always satisfied.

When the analysis method according to the present disclosure is used in the above-mentioned toilet bowl analysis device, back data can be acquired and stored in advance in the steps from S11 to S14 before shipment from the factory. The storage destination may be a memory built into the device, a storage device such as a server installed in a remote location, or a storage area on the cloud.

Next, in a state where the light source 38 is at an unknown temperature Tx, the peak intensity Iy[NaCl] and reference intensity Iy[ref] are determined for the spectrum of the test sample containing NaCl at an unknown concentration Cy, and the reference intensity Iy The unknown temperature Tx is determined from [ref] (S15). The unknown temperature Tx is an example of an arbitrary measured environmental parameter, and the unknown concentration Cy is an example of an arbitrary concentration. FIG. 9 is a diagram for explaining this process, and is a graph of the spectrum obtained, with the horizontal axis representing the wavelength λ and the vertical axis representing the intensity I. This step is a step of optically analyzing the urine excreted by the subject using the above-mentioned toilet bowl analyzer.

In S15, the acquired reference intensity Iy[ref] is compared with the reference intensity Iy[ref] stored in association with Tx in S12 to determine the unknown temperature Tx. For example, the unknown temperature Tx is determined by the temperature Tx associated with the reference intensity Iy[ref] that is equal to the reference intensity Iy[ref] obtained in S15 among the reference intensities Iy[ref] stored as back data in S12. do. If a spectrum can be obtained in a state where both the temperature of the light source 38 and the concentration of NaCl are unknown through the above-mentioned detection process, the reference intensity Iy[NaCl] does not depend on the concentration of NaCl, so it can be obtained in S15 from the back data saved in S12. The temperature Tx at the time of spectrum measurement can be determined (see FIG. 9).

Next, among the coefficients α stored in association with the temperature Tx as back data in S13, the coefficient α corresponding to the temperature Tx determined in S15 is determined, and the coefficient α corresponding to the temperature Tx determined in S15 is multiplied by the peak intensity Iy[NaCl] determined in S15. Convert to Iy ₀ [NaCl] (S16) (see FIG. 9). This Iy ₀ [NaCl] corresponds to the peak intensity of the spectrum of the current inspection target at the temperature T ₀ . In S16, the unknown temperature determined in S15 is read as the temperature Tx corresponding to the unknown temperature, and the coefficient α stored in association with the temperature Tx in S13 is converted to the peak intensity Iy[NaCl] obtained in S15. is multiplied by the peak intensity Iy ₀ [NaCl] at the temperature T ₀ .

Next, the peak intensity Iy ₀ [NaCl] obtained by replacing in S16 is compared with the peak intensity Iy [NaCl] stored in S14 as back data in association with the concentration Cy, and the true concentration of NaCl in the detection target sample is determined. The concentration Cy (see FIG. 9) is determined (S17). For example, of the peak intensity Iy[NaCl] saved as back data in S14, the concentration Cy associated with Iy[NaCl], which is equal to Iy ₀ [NaCl] calculated in S16, is determined, and this determined concentration Cy is This is determined as the concentration Cy of NaCl in the current detection target.

According to the above-mentioned analysis method, the component concentration can be determined without the influence of light intensity fluctuations simply by bringing urine into contact with the ATR element. Here, the light amount fluctuation may be caused by a change in the temperature of the light source or by simple deterioration or failure of the light source. According to the present disclosure, by setting the temperature at the maximum light intensity as the reference temperature _T0 , and setting the temperature at the time of the light intensity decreased from the maximum light intensity as the second temperature Tx, only light intensity fluctuations due to temperature changes can be caused. In addition, the influence of light intensity fluctuations due to light source deterioration and the like can also be eliminated. That is, it is possible to correct the light amount fluctuation regardless of the cause of the light amount fluctuation.

In this embodiment, the peak intensity is used as the first wavelength, and the reference wavelength that is not affected by the concentration of the component to be inspected is used as the second wavelength, but the present invention is not limited thereto. The first wavelength and the second wavelength may be different wavelengths.

The analysis method according to the present disclosure has been explained above using an example of application to spectral analysis to examine the salt concentration in a urine sample during urination. However, the analysis method according to the present disclosure can be widely applied to spectroscopic analysis in general, and There are no restrictions on the test target or test components as long as the test target can be analyzed. For example, a similar analysis method can be used to examine the concentration of components in saliva as well as urine. By doing so, the concentration of saliva components can be determined. Furthermore, although the present disclosure has described optical analysis using a spectrum, it can be used in general spectroscopic analysis for determining the concentration of a component whose concentration and temperature are unknown. Furthermore, combinations of parameters are not limited to combinations of concentration and temperature, and those skilled in the art can easily understand combinations of frequency and concentration, frequency and temperature, etc. with reference to this disclosure.

(Effects of this disclosure)
According to the present disclosure, an analysis method that can use a measured value at any light amount and temperature as an indicator of a health level independent of the measurement environment in a spectrum that is light amount dependent and temperature dependent, and an analysis method that uses the analysis method. equipment can be provided.

DESCRIPTION OF SYMBOLS 10... Toilet device, 12... Toilet main body, 26... Analysis device, 26a... Analytical light incident surface, 26b... Analytical light exit surface, 26c... Urine contact surface, 34... Cylindrical member, 38... Light source, 40... Optical sensor, 42...Lens

Claims (7)

An analysis method for identifying the concentration of a component to be tested contained in an object to be tested by spectral analysis of a light beam transmitted through an optical element,
(a) In the first measurement environment parameter, the intensity at a first wavelength of the spectrum of the sample containing the test component at the first concentration and the intensity at a second wavelength different from the first wavelength are determined. acquiring and storing in association with the first measurement environment parameter;
(b) In a second measurement environment parameter different from the first measurement environment parameter, the intensity at the first wavelength and the second wavelength of the spectrum of the sample containing the component to be tested at the first concentration are determined. and storing the intensity in association with the second measurement environment parameter;
(c) determining the ratio of the intensity at the first wavelength in the first measurement environment parameter and the intensity at the first wavelength in the second measurement environment parameter; The process of linking and saving,
(d) in the first measurement environment parameter, obtain the intensity at the first wavelength of the spectrum of the sample containing the test component at a second concentration different from the first concentration; a step of storing in association with the second concentration;
(e) obtaining intensities at the first wavelength and the second wavelength for a spectrum of the test object containing the test component at an arbitrary concentration in an arbitrary measurement environment parameter;
(f) The intensity at the second wavelength obtained in the step (e) is linked and stored in the step (b) with the second measurement environment parameter. determining the arbitrary measurement environment parameter by comparing it with the
(g) The arbitrary measurement environment parameter determined in step (f) was read as the second measurement environment parameter, and the resultant was stored in association with the second measurement environment parameter in step (c). Multiplying the intensity of the first wavelength obtained in step (e) by the ratio to replace the intensity of the first wavelength in the first measurement environment parameter;
(h) Compare the intensity of the first wavelength obtained by replacing in the step (g) with the intensity at the first wavelength stored in association with the second concentration in the step (d). determining the arbitrary concentration by
An analysis method comprising: The analysis method according to claim 1, wherein the first wavelength is a wavelength at which the intensity of the component to be inspected reaches a peak intensity, and the second wavelength is a wavelength that is not affected by the concentration of the component to be inspected. In the step (b), a plurality of second measurement environment parameters are set, and for each of them, the intensity at the first wavelength and the intensity at the second wavelength are obtained, and the plurality of second measurement environment parameters are obtained. The analysis method according to claim 1 or 2, wherein the analysis method is stored in association with each of the two measurement environment parameters. In the step (d), a plurality of second concentrations are set, and the intensity at the first wavelength is obtained for each of the second concentrations, and the intensity is stored in association with each of the plurality of second concentrations. The analysis method according to any one of 1 to 3. The analysis method according to any one of claims 1 to 4, wherein the first measurement environment parameter and the second measurement environment parameter are temperature. An analysis device that specifies the concentration of the component to be tested using the analysis method according to any one of claims 1 to 5. A program for executing an analysis to identify the concentration of a component to be inspected by spectral analysis of a light beam transmitted through an optical element, the program comprising:
(a) In the first measurement environment parameter, obtain the intensity at the first wavelength and the intensity at the second wavelength of the spectrum of the sample containing the component to be tested at the first concentration, and set the first measurement environment parameter. The process of storing the information by linking it to the
(b) In a second measurement environment parameter different from the first measurement environment parameter, the intensity at the first wavelength and the second wavelength of the spectrum of the sample containing the test component at the first concentration are determined. a step of acquiring the intensity of and storing it in association with the second measurement environment parameter;
(c) determining the ratio of the intensity at the first wavelength in the first measurement environment parameter and the intensity at the first wavelength in the second measurement environment parameter; The process of linking and saving,
(d) in the first measurement environment parameter, obtain the intensity at the first wavelength of the spectrum of the sample containing the test component at a second concentration different from the first concentration; a step of storing in association with the second concentration;
(e) obtaining intensities at the first wavelength and the second wavelength for a spectrum of the test object containing the test component at an arbitrary concentration in an arbitrary measurement environment parameter;
(f) The intensity at the second wavelength obtained in the step (e) is linked and stored in the step (b) with the second measurement environment parameter. determining the arbitrary measurement environment parameter by comparing it with the
(g) The arbitrary measurement environment parameter determined in the step (f) was replaced with the second measurement environment parameter, and the result was stored in association with the second measurement environment parameter in the step (c). Multiplying the intensity of the first wavelength obtained in step (e) by the ratio and replacing it with the intensity of the first wavelength in the first measurement environment parameter;
(h) Compare the intensity of the first wavelength obtained by replacing in the step (g) with the intensity at the first wavelength stored in association with the second concentration in the step (d). determining the arbitrary concentration by
A program that causes a computer to execute

Images