JP7417723B2 - Composition for producing soy protein concentrate with reduced phytic acid and its uses - Google Patents

Description

The present invention relates to a composition for producing a soybean protein concentrate with reduced phytic acid and its uses.

Bean (soybean) protein is a low-cost vegetable protein that, in addition to being eaten as a soybean itself, can be converted into various forms and used in various food processing products such as soy flour, defatted soy meal. ), soy protein concentrate, soy protein isolate, and textured soy protein. Such soy protein products are widely used in various processed products such as meat, grains, confectionery, beverages, baby food, dairy products and so-called second generation soy foods due to their excellent nutritional, functional and economical properties. It is used as an additive in products and manufactured as a variety of secondary processed products.

However, soy protein usually contains phytic acid, which binds to nutritionally important trace metals such as calcium, magnesium, iron, and zinc to form poorly soluble compounds, and activates metal ions such as calcium. Since it is known to have an inhibitory effect on α-amylase and digestive enzymes such as trypsin or pepsin, it is preferable to remove it from foods.

Methods for removing phytic acid known to date include ultrafiltration, ion exchange resin methods, and chemical methods. The ultrafiltration method has the disadvantage that peptides accumulate on the membrane surface during operation, slowing down the filtration rate and requiring periodic cleaning. Phytic acid contains phosphoric acid residues and can be removed by treating the protein solution with an anion exchange resin using the ion exchange resin method, but soy protein is also significantly adsorbed to protein resins. The ion exchange resin method is complicated to operate and leads to a decrease in the amount of protein. As a chemical method, it is possible to extract and remove soybean protein by treating it with a strong acid such as hydrochloric acid or trichloroacetic acid, or a strong base such as sodium hydroxide, but this method also requires complicated manufacturing operations. However, processing of large amounts of waste fluid requires effort, increases production costs, and acid-base treatment denatures proteins, which negatively affects protein digestion.

Therefore, a composition that can be used to efficiently remove phytic acid from soy protein concentrate, a method for producing a soy protein concentrate from which phytic acid has been removed, and a replacement of fishmeal using the soy protein concentrate is provided. There is a need for the development of feed compositions that can.

US Patent Application Publication No. 2010/0279367

An object of the present application is to provide a composition for producing a soy protein concentrate with reduced phytic acid.

Another object of the present application is to provide a method for producing a soy protein concentrate with reduced phytic acid.

Another object of the present application is to provide a soybean protein concentrate with reduced phytic acid produced by reacting the composition for producing a soybean protein concentrate with reduced phytic acid.

Another object of the present application is to provide a feed composition comprising said reduced phytic acid soy protein concentrate.

As used herein, the term "Soy Protein Concentrate (SPC)" refers to a product in which the protein content of soybeans is concentrated by removing soluble non-protein substances from defatted soybeans. Soybean meal, which is a byproduct left over from the production of edible oil, can be used as a high-protein feed material with increased protein content. Soy protein concentrate, along with fermented soybean meal, is classified as a typical vegetable-based high-protein material, and can also be used as an alternative to fish meal, which is currently used as a major protein source. For example, soy protein concentrates can be made by eluting defatted soybeans with water near their isoelectric point (pH 4-5) or 20-80% ethanol to remove water-soluble and non-protein substances such as carbohydrates and The soybean protein concentrate may be a soybean protein-containing product containing 50 to 90% protein by weight on a dry basis from which water is removed.

As used herein, "about" or "approximately" generally refers to within 20% above or below a given value or range, within 10%, within 5%, within 4%, within 3%, within 2%. It can be interpreted as meaning including a value or range within %, within 1%, or within 0.5%.

As used herein, "protein solubility" refers to the degree to which a protein dissolves in water, and the more hydrophilic amino acids present on the surface, the more they interact with the ionic groups of the solvent, increasing the solubility of the protein.

As used herein, "a soy protein concentrate with reduced phytic acid" refers to one in which the phytic acid content is reduced compared to a soy protein concentrate raw material (or a soy protein concentrate raw material that does not contain phytase).

The soy protein concentrate with reduced phytic acid according to one example has a phytic acid content of about 35% or more, 40% or more, 50% or more, 60% or more, 70% or more, 75% or more, or 80% or more based on the soybean protein concentrate raw material. % or more, 0.1-100%, 1-100%, 10-100%, 20-100%, 25-100%, 30-100%, 35-100%, 40-100%, 50-100%, 60-100%, 65-100%, 70-100%, 0.1-90%, 1-90%, 10-90%, 20-90%, 25-90%, 30-90%, 35-90 %, 40-90%, 50-90%, 60-90%, 65-90%, 70-90%, 0.1-85%, 1-85%, 10-85%, 20-85%, 25 ~85%, 30-85%, 35-85%, 40-85%, 50-85%, 60-85%, 65-85%, 70-85%, 0.1-80%, 1-80% , 10-80%, 20-80%, 25-80%, 30-80%, 35-80%, 40-80%, 50-80%, 60-80%, 65-80%, 70-80% , 0.1-75%, 1-75%, 10-75%, 20-75%, 25-75%, 30-75%, 35-75%, 40-75%, 50-75%, 60-75% It can be reduced by 75%, 65-75%, or 70-75%.

Phytic acid is IP6 (myo-inositol hexaphosphate) and is composed of a myo-inositol ring and six phosphate groups, and these phosphate groups are attached symmetrically. . It is present in plants, especially cereals and leguminous plants. Phytic acid reduces the nutritional value of soy protein by forming complexes with proteins and reduces the action of polyvalent metal ions such as calcium, iron, and zinc, preventing them from being absorbed into the human body. However, in the stomach and intestines, pepsin or trypsin interfere with the activity of digestive enzymes, so a soybean protein concentrate with reduced phytic acid can be usefully used as a nutritionally superior food additive due to its low phytic acid content. can.

The present invention will be explained in more detail below.
One aspect can provide a composition for producing a reduced phytic acid soy protein concentrate (or a composition for reducing the phytic acid of a soy protein concentrate) comprising a soy protein concentrate and a phytase.

As used herein, ``a composition for producing a soybean protein concentrate with reduced phytic acid'' may mean the same thing as ``a composition for reducing (reducing) phytic acid in a soybean protein concentrate.'' .

The composition for producing a soybean protein concentrate with reduced phytic acid according to an example includes phytase, and the content of the phytase is 0 based on the weight of the soybean protein concentrate (e.g., soybean protein concentrate raw material). .001-10% by weight, 0.005-10% by weight, 0.01-10% by weight, 0.025-10% by weight, 0.03-10% by weight, 0.05-10% by weight, 0.075 ~10% by weight, 0.1-10% by weight, 0.25-10% by weight, 0.3-10% by weight, 0.5-10% by weight, 0.001-5% by weight, 0.005-5 Weight%, 0.01-5% by weight, 0.025-5% by weight, 0.03-5% by weight, 0.05-5% by weight, 0.075-5% by weight, 0.1-5% by weight , 0.25-5% by weight, 0.3-5% by weight, 0.5-5% by weight, 0.001-1% by weight, 0.005-1% by weight, 0.01-1% by weight, 0 .025-1% by weight, 0.03-1% by weight, 0.05-1% by weight, 0.075-1% by weight, 0.1-1% by weight, 0.25-1% by weight, 0.3 ~1% by weight, 0.5-1% by weight, 0.001-0.5% by weight, 0.005-0.5% by weight, 0.01-0.5% by weight, 0.025-0.5 Weight%, 0.03-0.5% by weight, 0.05-0.5% by weight, 0.075-0.5% by weight, 0.1-0.5% by weight, 0.25-0.5 Weight%, 0.3-0.5% by weight, 0.5-0.5% by weight, 0.001-0.3% by weight, 0.005-0.3% by weight, 0.01-0.3 Weight%, 0.025-0.3% by weight, 0.03-0.3% by weight, 0.05-0.3% by weight, 0.075-0.3% by weight, 0.1-0.3 Weight%, 0.25-0.3% by weight, 0.001-0.25% by weight, 0.005-0.25% by weight, 0.01-0.25% by weight, 0.025-0.25 %, 0.03-0.25%, 0.05-0.25%, 0.075-0.25%, or 0.1-0.25% by weight.

The phytase is a type of phosphoric acid hydrolase that hydrolyzes phytic acid to produce myo-inositol and inorganic phosphate, and according to one example, a composition containing phytase can effectively reduce the phytic acid content of soy protein concentrate. can be reduced.

In the case of a composition containing phytase in the content within the above range, when producing a soybean protein concentrate with reduced phytic acid, the phytic acid content can be efficiently reduced relative to the amount of phytase added. can economically produce a soy protein concentrate with reduced phytic acid.

A composition for producing a soy protein concentrate with reduced phytate containing phytase within the range described above has an excellent (significantly) reduced content of soy protein concentrate ( 1) a decrease in phytic acid content; (2) an increase in free phosphorus content; and/or (3) an increase in digestibility can be induced.

In one example, the water content of the composition for producing a soybean protein concentrate with reduced phytic acid is 20% by weight or more, 30% by weight or more, 40% by weight or more, 45% by weight based on the weight of the entire composition. 50% by weight or more, 55% by weight or more, 95% by weight or less, 90% by weight or less, 85% by weight or less, 80% by weight or less, 75% by weight or less, 70% by weight or less, 65% by weight or less, 60% by weight The following: 30-95% by weight, 35-95% by weight, 40-95% by weight, 45-95% by weight, 50-95% by weight, 55-95% by weight, 30-90% by weight, 35-90% by weight, 40-90% by weight, 45-90% by weight, 50-90% by weight, 55-90% by weight, 30-85% by weight, 35-85% by weight, 40-85% by weight, 45-85% by weight, 50- 85% by weight, 55-85% by weight, 30-80% by weight, 35-80% by weight, 40-80% by weight, 45-80% by weight, 50-80% by weight, 55-80% by weight, 30-75% by weight %, 35-75 wt%, 40-75 wt%, 45-75 wt%, 50-75 wt%, 55-75 wt%, 30-70 wt%, 35-70 wt%, 40-70 wt%, 45-70% by weight, 50-70% by weight, 55-70% by weight, 30-65% by weight, 35-65% by weight, 40-65% by weight, 45-65% by weight, 50-65% by weight, 55- 65% by weight, 30-60% by weight, 35-60% by weight, 40-60% by weight, 45-60% by weight, 50-60% by weight, 55-60% by weight, 30-55% by weight, 35-55% by weight %, 40-55%, 45-55%, or 50-55% by weight.

A composition for producing a soy protein concentrate with reduced phytic acid having a water content within the range described above is superior (significantly) in producing a soy protein concentrate than a composition having a water content outside the range. (1) decrease in phytic acid content; (2) increase in free phosphorus content; and/or (3) increase in digestibility.

In one example, the pH of the composition for producing a soybean protein concentrate with reduced phytic acid is 7 or less, 6.8 or less, 6.5 or less, 6 or less, 5.5 or less, 5 or less, 4.75 or less. , 4.5 or less, 4 or less, 1 or more, 1.5 or more, 2 or more, 2.5 or more, 3 or more, 3.5 or more, 4 or more, 4.5 or more, pH 2 to 7, pH 2.5 to 7 , pH3-7, pH3.5-7, pH4-7, pH4.25-7, pH4.5-7, pH2-6.8, pH2.5-6.8, pH3-6.8, pH3.5 ~6.8, pH4~6.8, pH4.25~6.8, pH4.5~6.8, pH2~6.5, pH2.5~6.5, pH3~6.5, pH3.5 ~6.5, pH4-6.5, pH4.25-6.5, pH4.5-6.5, pH2-6, pH2.5-6, pH3-6, pH3.5-6, pH4-6 , pH4.25-6, pH4.5-6, pH2-5.5, pH2.5-5.5, pH3-5.5, pH3.5-5.5, pH4-5.5, pH4.25 ~5.5, pH4.5~5.5, pH2~5, pH2.5~5, pH3~5, pH3.5~5, pH4~5, pH4.25~5, pH4.5~5, pH2 ~4.75, pH2.5~4.75, pH3~4.75, pH3.5~4.75, pH4~4.75, pH4.25~4.75, pH4.5~4.75, pH2 ~4.5, pH 2.5-4.5, pH 3-4.5, pH 3.5-4.5, pH 4-4.5, or pH 4.25-4.5.

Compositions for making soy protein concentrates with reduced phytic acid having a pH within the range described above are superior (significantly) in producing soy protein concentrates to compositions having a pH outside the above ranges. 1) a decrease in phytic acid content; (2) an increase in free phosphorus content; and/or (3) an increase in digestibility can be induced.

According to one example, the composition for producing a soybean protein concentrate with reduced phytic acid may be reacted and dried to produce a soybean protein concentrate with reduced phytic acid. A composition for producing a soybean protein concentrate with reduced phytic acid according to one example effectively reduces phytic acid while increasing the proportion of low protein peptides to increase digestibility in animals and improve quality control. A simple soy protein concentrate can be produced that can be added to feed to increase animal digestibility and increase phosphorus content without additional phosphorus addition.

Another aspect provides a method of making a reduced phytic acid soy protein concentrate, the method comprising reacting the reduced phytic acid soy protein concentrate composition.

Reacting the composition for producing a soybean protein concentrate with reduced phytic acid may be carried out at 20-80°C, 25-80°C, 30-80°C, 40-80°C, 50-80°C, 55-80°C. , 20-70℃, 25-70℃, 30-70℃, 40-70℃, 50-70℃, 55-70℃, 20-65℃, 25-65℃, 30-65℃, 40-65℃ , at a temperature of 50-65°C, 55-65°C, 20-60°C, 25-60°C, 30-60°C, 40-60°C, 50-60°C, or 55-60°C,
0.5-24 hours, 1-24 hours, 2-24 hours, 3-24 hours, 4-24 hours, 0.5-18 hours, 1-18 hours, 2-18 hours, 3-18 hours, 4 ~18 hours, 0.5-12 hours, 1-12 hours, 2-12 hours, 3-12 hours, 4-12 hours, 0.5-8 hours, 1-8 hours, 2-8 hours, 3- The composition may be incubated (or reacted) for 8 hours, 4-8 hours, 0.5-6 hours, 1-6 hours, 2-6 hours, 3-6 hours, or 4-6 hours. .

If the enzymatic reaction is carried out under conditions within the above range, a soybean protein concentrate with effectively reduced phytic acid can be produced even without protein denaturation of the soybean protein concentrate.

The manufacturing method may additionally include the step of drying the reactant reacted in the step. The drying can be performed by normal temperature ventilation drying, hot air drying, vacuum drying, spray drying, and/or freeze drying. The drying process can terminate the enzymatic reaction of phytase.

The manufacturing method may additionally include the step of pulverizing the dried soy protein concentrate after the drying step, and the pulverizing step may include pulverizing the soy protein concentrate into various sizes depending on the purpose for which the soy protein concentrate is used. For example, it can be ground using a hammer mill.

Other aspects can provide a method of making a reduced phytic acid soy protein concentrate, including the steps of:
Adding acid to the soy protein concentrate to adjust the pH of the soy protein concentrate to below 6.8; and adding phytase and water to adjust the final moisture content of the soy protein concentrate to 40% by weight or higher. step.

Another aspect is to add a mixture containing phytase, water, and/or acid to the soy protein concentrate to adjust the water content of the soy protein concentrate to 40% by weight or higher, and to adjust the pH of the soy protein concentrate to 6.5% by weight. It is possible to provide a method for producing a soy protein concentrate with reduced phytic acid, the method comprising adjusting the soybean protein concentrate to 8 or less.

Other aspects include adding a mixture containing phytase, water, and/or acid to the soy protein concentrate to adjust the pH of the soy protein concentrate to below 6.8, and additionally adding phytase and/or water. and adjusting the final moisture content of the soy protein concentrate to 40% by weight or more.

According to one example, the soybean protein concentrate used as the raw material before adjusting the moisture content and/or pH is continuously sourced from the same type of soybean from the same region so as to maintain the same quality. It can be used after receiving the supply. For example, soy protein concentrate can be prepared by washing soy protein with alcohol, extracting soybean oil using an organic solvent such as hexane, and then adding additional alcohol from the remaining defatted soybean meal. This can be done by removing non-protein components such as water-soluble and water-insoluble carbohydrates other than proteins.

According to one example, adjusting the water content may be performed by adding phytase and water to the soy protein concentrate, or by adding water to the soy protein concentrate. The step of adjusting the moisture content includes adjusting the moisture content of the soy protein concentrate based on the weight of the entire composition (e.g., the total weight of the soy protein concentrate including added phytase, water, and/or acid). , 20% by weight or more, 30% by weight or more, 40% by weight or more, 45% by weight or more, 50% by weight or more, 55% by weight or more, 95% by weight or less, 90% by weight or less, 85% by weight or less, 80% by weight or less , 75% by weight or less, 70% by weight or less, 65% by weight or less, 60% by weight or less, 30-95% by weight, 35-95% by weight, 40-95% by weight, 45-95% by weight, 50-95% by weight , 55-95% by weight, 30-90% by weight, 35-90% by weight, 40-90% by weight, 45-90% by weight, 50-90% by weight, 55-90% by weight, 30-85% by weight, 35 ~85% by weight, 40-85% by weight, 45-85% by weight, 50-85% by weight, 55-85% by weight, 30-80% by weight, 35-80% by weight, 40-80% by weight, 45-80 Weight%, 50-80% by weight, 55-80% by weight, 30-75% by weight, 35-75% by weight, 40-75% by weight, 45-75% by weight, 50-75% by weight, 55-75% by weight , 30-70% by weight, 35-70% by weight, 40-70% by weight, 45-70% by weight, 50-70% by weight, 55-70% by weight, 30-65% by weight, 35-65% by weight, 40 ~65% by weight, 45-65% by weight, 50-65% by weight, 55-65% by weight, 30-60% by weight, 35-60% by weight, 40-60% by weight, 45-60% by weight, 50-60 % by weight, 55-60% by weight, 30-55% by weight, 35-55% by weight, 40-55% by weight, 45-55% by weight, or 50-55% by weight.

According to one example, in the step of adjusting the moisture content of the soy protein concentrate, the moisture content can be measured by a commonly used method, such as a drying method (e.g., normal pressure heat drying method and/or high temperature heat drying method). etc.), a distillation method, an electric moisture meter method, a near-infrared spectroscopic absorption method, a gas chromatography method, a nuclear magnetic resonance absorption method, a volumetric titration method, and/or a coulometric titration method. The moisture content of soy protein concentrate (or soy protein concentrate raw material) before the moisture content is adjusted is 5-30% by weight, 5-25% by weight, 5-20% by weight, 5-15% by weight, 5% by weight. It can be from 12% to 12%, 5% to 10%, 6% to 9%, 7% to 8%, 15% to 25%, 20% to 25%, or 20% to 30%.

According to one example, in the step of adjusting the pH of the soy protein concentrate, the pH may be 7 or less, 6.8 or less, 6.5 or less, 6 or less, 5.5 or less, 5 or less, 4.75 or less, 4. 5 or less, 4 or less, 1 or more, 1.5 or more, 2 or more, 2.5 or more, 3 or more, 3.5 or more, 4 or more, 4.5 or more, pH 2-7, pH 2.5-7, pH 3- 7, pH 3.5-7, pH 4-7, pH 4.25-7, pH 4.5-7, pH 2-6.8, pH 2.5-6.8, pH 3-6.8, pH 3.5-6. 8, pH 4-6.8, pH 4.25-6.8, pH 4.5-6.8, pH 2-6.5, pH 2.5-6.5, pH 3-6.5, pH 3.5-6. 5, pH 4-6.5, pH 4.25-6.5, pH 4.5-6.5, pH 2-6, pH 2.5-6, pH 3-6, pH 3.5-6, pH 4-6, pH 4. 25-6, pH4.5-6, pH2-5.5, pH2.5-5.5, pH3-5.5, pH3.5-5.5, pH4-5.5, pH4.25-5. 5, pH 4.5-5.5, pH 2-5, pH 2.5-5, pH 3-5, pH 3.5-5, pH 4-5, pH 4.25-5, pH 4.5-5, pH 2-4. 75, pH 2.5-4.75, pH 3-4.75, pH 3.5-4.75, pH 4-4.75, pH 4.25-4.75, pH 4.5-4.75, pH 2-4. 5, pH can be adjusted to 2.5 to 4.5, pH 3 to 4.5, pH 3.5 to 4.5, pH 4 to 4.5, or pH 4.25 to 4.5.

In one example, the acid added during the step of adjusting the pH of the soy protein concentrate may be hydrochloric acid, sulfuric acid, nitric acid and/or acetic acid, and may be in the form of an aqueous solution.

According to one embodiment, 40 ml of 1N HCl (or 1.458 g of 100% HCl) to adjust the pH of 100 g of soy protein concentrate raw material to 4.5, 33.0 ml of 1 N HCl to adjust the pH to 4.75. Add 5 ml (or 1.221 g of 100% HCl), 28 ml (or 1.021 g of 100% HCl) to adjust pH to 5, 15 ml of 1N HCl (or 0.547 g of 100% HCl) to adjust to pH 5.5 The type and amount of acid added can be changed depending on the pH of the raw material.

According to one example, the pH of the soy protein concentrate (or the source of the soy protein concentrate) before the pH is adjusted is 5-8, 6-7.5, 6.5-7, or 6.5-8. 6.8.

According to one example, the manufacturing method may additionally include reacting (incubating), drying, and/or grinding the moisture content and pH adjusted soy protein concentrate. The reaction, drying, and/or milling steps are as described above.

According to one example, the "moisture content and pH adjusted soy protein concentrate" may be the same as the "composition for producing a soy protein concentrate with reduced phytic acid."

According to one example, the phytic acid reduced soy protein concentrate contains less than 2%, less than 1.9%, less than 1.8%, less than 1.7%, 1.6% by weight of phytic acid. less than 1.5% by weight, less than 1.4% by weight, less than 1.3% by weight, less than 1.2% by weight, less than 1.1% by weight, less than 1% by weight, less than 0.7% by weight , less than 0.6% by weight, less than 0.5% by weight, less than 0.4% by weight, less than 0.3% by weight, less than 0.2% by weight, 2% by weight or less, 1.9% by weight or less, 1. 8% by weight or less, 1.7% by weight or less, 1.6% by weight or less, 1.5% by weight or less, 1.4% by weight or less, 1.3% by weight or less, 1.2% by weight or less, 1.1 Weight % or less, 1 weight % or less, 0.7 weight % or less, 0.6 weight % or less, 0.5 weight % or less, 0.4 weight % or less, 0.3 weight % or less, 0.2 weight % or less , 0.01-2% by weight, 0.05-2% by weight, 0.1-2% by weight, 0.2-2% by weight, 0.3-2% by weight, 0.01-1.5% by weight , 0.05-1.5% by weight, 0.1-1.5% by weight, 0.2-1.5% by weight, 0.3-1.5% by weight, 0.01-1% by weight, 0 .05-1% by weight, 0.1-1% by weight, 0.2-1% by weight, 0.3-1% by weight, 0.01-0.8% by weight, 0.05-0.8% by weight , 0.1-0.8% by weight, 0.2-0.8% by weight, 0.3-0.8% by weight, 0.01-0.7% by weight, 0.05-0.7% by weight , 0.1-0.7% by weight, 0.2-0.7% by weight, 0.3-0.7% by weight, 0.01-0.6% by weight, 0.05-0.6% by weight , 0.1-0.6% by weight, 0.2-0.6% by weight, 0.3-0.6% by weight, 0.01-0.5% by weight, 0.05-0.5% by weight , 0.1-0.5% by weight, 0.2-0.5% by weight, 0.3-0.5% by weight, 0.01-0.4% by weight, 0.05-0.4% by weight , 0.1-0.4% by weight, 0.2-0.4% by weight, 0.3-0.4% by weight, 0.01-1.6% by weight, 0.1-1.5% by weight , 0.1-1.4% by weight, 0.1-1% by weight, 0.1-0.5% by weight, 0.1-0.4% by weight, 0.1-0.3% by weight, 0 .2-1.4% by weight, 0.3-1.4% by weight, 0.2-1.2% by weight, 0.2-1% by weight, 0.2-0.8% by weight, 0.2 ~0.6% by weight, 0.2-0.5% by weight, 0.2-0.4% by weight, 0.5-0.5% by weight, 0.3-0.4% by weight, 0.4 ~2% by weight, 0.4-1.5% by weight, 0.4-1% by weight, 0.4-0.8% by weight, 0.4-0.7% by weight, 0.4-0.6 % by weight, or 0.4-0.5% by weight. The content of phytic acid in the phytic acid reduced soy protein concentrate is based on the total weight of the phytic acid reduced soy protein concentrate (or the phytic acid reduced soy protein concentrate in dried form). (based on the powder weight of the concentrate).

According to one example, the phytic acid reduced soy protein concentrate may contain free phosphorus from 0.1 to 2% by weight, from 0.2 to 2% by weight, from 0.3 to 2% by weight, from 0.4 to 2% by weight. Weight%, 0.5-2% by weight, 0.4-2% by weight, 0.1-1.5% by weight, 0.2-1.5% by weight, 0.3-1.5% by weight, 0 .4-1.5% by weight, 0.5-1.5% by weight, 0.4-1.5% by weight, 0.1-1% by weight, 0.2-1% by weight, 0.3-1 Weight%, 0.4-1% by weight, 0.5-1% by weight, 0.4-1% by weight, 0.1-0.8% by weight, 0.2-0.8% by weight, 0.3 ~0.8% by weight, 0.4-0.8% by weight, 0.5-0.8% by weight, 0.4-0.8% by weight, 0.1-0.7% by weight, 0.2 ~0.7% by weight, 0.3-0.7% by weight, 0.4-0.7% by weight, 0.5-0.7% by weight, 0.4-0.7% by weight, 0.1 ~0.6% by weight, 0.2-0.6% by weight, 0.3-0.6% by weight, 0.4-0.6% by weight, 0.5-0.6% by weight, or 0. It can be contained in an amount of 4 to 0.6% by weight. The content of free phosphorus in the phytic acid reduced soy protein concentrate is based on the total weight of the phytic acid reduced soy protein concentrate (or the phytic acid reduced soy protein concentrate in dried form). (based on the powder weight of the concentrate).

According to one example, the soybean protein concentrate with reduced phytic acid contains peptides (proteins) in an amount of 40 to 95% by weight, 45 to 95% by weight, 50 to 90% by weight, based on the dried powder. It may contain 55-80%, 50-70%, 50-60%, or 60-70% by weight.

According to one example, the peptide content of the soybean protein concentrate with reduced phytic acid is similar to the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material, and the peptide content is similar to that of the soybean protein concentrate used as a raw material. ) may be higher than the soy protein concentrate used as raw material. The content of low-molecular-weight peptides (e.g., peptides less than 30 kDa) contained in the soybean protein concentrate with reduced phytic acid according to one example is increased from that of the raw material, and the soybean protein concentrate with reduced phytic acid produced by the above method is It can be highly digestible.

According to one example, the phytic acid reduced soy protein concentrate contains peptides less than 30 kDa by 10% to 95%, 15% to 90%, 15% based on the total peptides contained in the concentrate. ~85%, 15%~80%, 20%~90%, 20%~80%, 30%~90%, 30%~80%, 30%~70%, 30%~65%, 30%~60 %, 30% to 55%, 30% to 50%, 30% to 45%, 30% to 40%, 20% to 40%, or 25% to 40%.

According to one example, the phytic acid-reduced soy protein concentrate contains 10 to 95%, 15 to 90%, 15 to 90% by weight of peptides less than 30 kDa based on the total peptides contained in the concentrate. 85% by weight, 15-80% by weight, 20-90% by weight, 20-80% by weight, 30-90% by weight, 30-80% by weight, 30-70% by weight, 30-65% by weight, 30-60% by weight %, 30-55%, 30-50%, 30-45%, 30-40%, 20-40%, or 25-40%.

The peptide refers to a polymer in which various combinations of amino acids form a peptide bond. Low molecular weight peptides have a small molecular weight and have the advantage of being easily absorbed during digestion and increasing digestibility when used as animal feed. The higher the content of low molecular weight peptides in the feed, the higher the animal's digestibility can be improved. In one example, the low molecular weight peptide may refer to a peptide having a molecular weight of less than 40 kDa, less than 30 kDa, or less than 25 kDa.

The soybean protein concentrate with reduced phytic acid produced using the composition for producing a soybean protein concentrate according to one example has an increased content ratio of peptides less than 30 kDa among the peptides contained therein. By using the composition for producing a soy protein concentrate according to the present invention or by using the production method according to one example, a soy protein concentrate having improved digestibility and a feed composition containing the same can be produced. According to one example, GPC (Gel Permeation Chromatography) can be used to determine the protein molecular weight profile of a soy protein concentrate with reduced phytic acid.

According to one example, said phytic acid reduced soy protein concentrate may be for use in animal feed manufacturing.

Said animal may be a mammal, poultry, fish and/or crustacean. Said mammals include pigs, cows, horses, deer, goats, dogs, cats, and/or rabbits, said poultry include chickens, ducks, geese, and/or turkeys, and said fish and crustaceans include trout, salmon. , and/or shrimp.

In one example, the soybean protein concentrate with reduced phytic acid may have increased digestibility (eg, phosphorus digestibility) compared to the soybean protein concentrate used as a raw material.

As used herein, "digestibility" can mean the fraction of nutrients that disappear from the gastrointestinal tract or a particular segment of the gastrointestinal tract, such as the small intestine, and is referred to as "nutrient digestibility" and/or "nutrient digestibility". can be used with the same meaning. Nutrient digestibility is the difference between what is administered to a subject and what is excreted in the subject's stool, or the difference between what is administered to a subject and what remains in the digesta of a particular segment of the gastrointestinal tract, e.g., the ileum. It can be measured by the difference between things. In one example, nutrient digestibility can be measured by the difference between the nutrient intake and the nutrient excreted by the total collection of waste (feces) over a period of time; or by using an inert marker (or indicator) that is not absorbed by the animal. The amount of nutrients lost in the entire gastrointestinal tract or segments of the gastrointestinal tract can then be calculated and measured. Such inert markers (or indicators) may be titanium dioxide, chromium oxide, and/or acid-insoluble ash. In one example, digestibility can be expressed as a percentage of feed nutrient or mass units of digestible nutrient per weight unit of feed nutrient. In one example, nutrient digestibility can include starch digestibility, fat digestibility, protein digestibility, mineral digestibility, phosphorus digestibility, and/or amino acid digestibility.

In one example, nutrient digestibility can be calculated by Equation 1 below, where the nutrients below can be starch, carbohydrate, fat, protein, mineral, and/or phosphorus.

(Formula 1)
Nutrient digestibility = 100 x (1 - (indicator amount in feed/indicator amount in feces) x (nutrient content in feces/nutrient content in feed))

Another aspect can provide a soy protein concentrate with reduced phytic acid produced by the above production method.

Another aspect may provide a reduced phytic acid soy protein concentrate obtained by reacting the composition for producing a reduced phytic acid soy protein concentrate.

In another aspect, a soybean protein concentrate with reduced phytic acid obtained by reacting and drying the composition for producing a soybean protein concentrate with reduced phytic acid can be provided.

The soybean protein concentrate with reduced phytic acid is as described above. The reaction and/or drying of the composition is as described above.

As an example, soybean protein concentrate with reduced phytic acid is a typical vegetable-based high-protein material and can be used as an alternative to fishmeal, which has been used as the main protein source. In the case of fish meal, the price fluctuates widely depending on the catch of the raw material fish, and it is difficult to maintain uniform quality depending on the condition of the fish.On the other hand, soy protein concentrate can be made from soybeans, which are plant-based raw materials, and quality control is possible. Therefore, the volatility of supply and demand is relatively low and it can be used at stable prices. Existing soy protein concentrates contain multiple high-molecular proteins with a molecular weight of 1,000 kDa or more consisting of protein subunits and various anti-nutritional factors (ANFs) that inhibit digestion. However, the soybean protein concentrate with reduced phytic acid has lower phytic acid content and higher content of low-protein peptide than the raw material, which can increase digestibility.

Other aspects can provide feed compositions comprising the reduced phytic acid soy protein concentrate. Another aspect can provide a feed composition comprising a reduced phytic acid soy protein concentrate produced by the above production method. The ``phytic acid-reduced soybean protein concentrate'' contained in the feed composition is as described above.

In this application, "feed composition" means a substance that provides the organic or inorganic nutrients necessary for sustaining the life of an individual and feeding said individual. The feed composition may contain nutrients such as energy, protein, fat, vitamins, minerals, etc. that are required by the individual that consumes the feed, and may include, but is not limited to, grains, roots, and food processing by-products. Plant feeds such as algae, fibrous substances, oils and fats, starches, evening globules, grain by-products, or proteins, inorganic substances, oils and fats, mineral substances, single-cell proteins, zooplankton, fishmeal, etc. Can be used as animal feed. In the present application, the feed composition is a concept that includes all substances added to feed (ie, feed additives), feed raw materials, and feed itself fed to individuals.

The above-mentioned individual means a breeding subject, and includes any living organism that can ingest the feed of the present application without any restriction. The feed composition according to one example can be applied to a number of animal diets, including mammals, poultry, fish, and/or crustaceans. Said mammals include pigs, cows, horses, deer, goats, dogs, cats, and/or rabbits, said poultry include chickens, ducks, geese, and/or turkeys, and said fish and crustaceans include trout, salmon. , and/or shrimp.

According to one example, the feed composition may additionally contain organic acids such as citric acid, fumaric acid, adipic acid, lactic acid; Phosphates such as sodium and polymerized phosphate; and one or more of natural antioxidants such as polyphenols, catechins, tocopherols, vitamin C, green tea extract, chitosan, and tannic acid may be used in combination. If necessary, other conventional additives such as anti-influenza agents, buffers, and/or bacteriostatic agents can be added. Additionally, diluents, dispersants, surfactants, binders, and/or lubricants may be added to produce injectable dosage forms such as aqueous solutions, suspensions, emulsions, etc., capsules, granules or tablets. It can be formulated into

According to one example, the feed composition may include various adjuvants such as amino acids, inorganic salts, vitamins, antioxidants, antifungal agents, antibacterial agents, etc., as auxiliary ingredients, ground or crushed wheat, barley, corn, etc. In addition to the main ingredients such as vegetable protein feeds, animal protein feeds such as blood meal, meat meal, fish meal, animal fats and vegetable fats, nutritional supplements, growth promoters, digestive absorption enhancers, and/or disease prevention. Can be used with agents.

When the feed composition according to one example is used as a feed additive, the feed composition can be added as is or used in conjunction with other ingredients, and can be appropriately used in a conventional manner. Dosage forms of the feed composition can be combined with non-toxic pharmaceutically acceptable carriers to produce immediate or sustained release dosage forms. Such edible carriers can be corn starch, lactose, sucrose, propylene glycol. In the case of solid carriers, the dosage forms may be tablets, powders, troches, etc., and in the case of liquid carriers, the dosage forms may be syrups, liquid suspensions, emulsions, solutions, etc. The dosage form may also contain preservatives, humectants, solution promoters, and stabilizers.

A feed composition according to an example may include the reduced phytic acid soy protein concentrate in an amount of about 1 to 500 g per kg, or 100 to 500 g on a dry weight basis.

In one example, the feed composition includes one or more selected from the group consisting of fishmeal, wheat flour, soybean meal, soybean lecithin, fish oil, vitamins, and minerals in addition to the soybean protein concentrate with reduced phytic acid. can be included. For example, the feed composition according to one example includes 10 to 50 parts by weight of the soybean protein concentrate with reduced phytic acid, 10 to 40 parts by weight of fish meal, 10 to 40 parts by weight of wheat flour, and 10 to 30 parts by weight of soybean meal. part, soybean lecithin in an amount of 0.1 to 10 parts by weight, fish oil in an amount of 0.1 to 10 parts by weight, vitamins in an amount of 0.01 to 1 part by weight, and minerals in an amount of 0.01 to 1 part by weight.

Ｆｅ－Ｃｉｔｒａｔｅ、ＭｎＳＯ_４、ＦｅＳＯ_４、ＣｕＳＯ_４、ヨード酸カルシウム（Calciumiodate）、ＭｇＯ、および／またはＮａＳｅＯ_３であり得る。 In one example, the vitamins include Ascorbic acid, DL-Calcium pantothenate, Choline bitrate, Inositol, Menadion, Niacin, and Hydrochloric acid. Pyridoxine HCl, Riboflavin, Thiamine mononitrate, DL-α-Tocopherol acetate, Retinyl acetate, Biotin, Folic acid ), and/or Cobalamin. In one example, the minerals include NaCl,

It can be Fe-Citrate, MnSO ₄ , FeSO ₄ , CuSO ₄ , Calciumiodate, MgO, and/or NaSeO ₃ .

According to one example, the reduced phytic acid soy protein concentrate may be mixed with additional feed ingredients and auxiliary ingredients and then fed in a mash or subjected to additional processing steps such as pelletizing, expanding and/or extrusion steps. can go through.

The feed composition according to one example may have digestibility that is equal to or better than the raw soy protein concentrate and/or fishmeal. For example, the feed composition has a digestibility (e.g., phosphorus digestibility) of 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 60% to 99.9%, 60% or more, %~99%, 60%~95%, 60%~90%, 60%~85%, 65%~99.9%, 65%~99%, 65%~95%, 65%~90%, 65 %~85%, 70%~99.9%, 70%~99%, 70%~95%, 70%~90%, 70%~85%, 75%~99.9%, 75%~99% , 75% to 95%, 75% to 90%, 75% to 85%, 80% to 99.9%, 80% to 99%, 80% to 95%, 80% to 90%, or 80% to 85 %. The phosphorus digestibility may be calculated using Equation 1 or Equation 3.

Another aspect provides a feed composition (e.g., feed additive) for promoting (or increasing) digestibility (e.g., phosphorus digestibility), comprising the reduced phytic acid soy protein concentrate. be able to.

Another aspect can provide a fish meal replacement feed composition (eg, a feed additive) comprising the reduced phytic acid soy protein concentrate.

Other aspects can provide a method of increasing digestibility (eg, phosphorus digestibility) comprising administering to an animal a feed additive and/or feed composition according to one example.

By using the composition for producing a soybean protein concentrate with reduced phytic acid according to one example, or according to the production method according to one example, a soybean protein concentrate with reduced phytic acid can be efficiently produced, and an animal Soybean protein concentrate with reduced phytic acid, which can increase the content of available phosphorus and increase the content ratio of low-molecular-weight peptides to improve animal digestibility of soybean protein concentrate, as an example A feed composition containing the above may have excellent digestibility.

Hereinafter, the present invention will be explained in more detail through Examples. However, these Examples are for illustratively explaining the present invention, and the scope of the present invention is not limited to these Reference Examples and Examples.

[Example 1]
Method for producing soy protein concentrate with reduced phytic acid The raw material for soy protein concentrate was the X-soy 600 product supplied by CJ Selecta's own affiliate company.

After drying the soy protein concentrate raw material in a 105° C. dry oven overnight, the moisture content of the raw material was determined by measuring the weight before/after drying. The pH of the raw material was determined by mixing the raw material and water (90% by weight) to make a 10% by weight solution, and then measuring the pH of the solution. The soy protein concentrate raw material contained approximately 60% protein by weight, the moisture content was approximately 7-8% by weight, and the pH was 6.5-6.8.

After confirming the water content and pH of the raw materials, the amount of water added and the amount of hydrochloric acid to be added were calculated to match each enzyme reaction condition. The final moisture content was calculated according to the following equation 2, and the amount of hydrochloric acid added according to the following equation 2 was calculated according to the target pH.

(Formula 2)
Final moisture content % (wt%) = (raw material weight (g) ) + amount of water added (g) + amount of hydrochloric acid added (g)) x 100

By adding hydrochloric acid and/or water to the soy protein concentrate raw material, the water content of the composition containing the soy protein concentrate is 40 to 60% by weight based on the total weight of the composition, and the pH is 4.5 to 6.8. Adjusted. Hydrochloric acid was used to adjust the pH of the composition containing the soy protein concentrate, and only water was added to the reactant having a pH of 6.8. In this example, 1N HCl was used to adjust the pH of the reaction raw materials. 40 ml of 1 N HCl to adjust the pH of 100 g of soy protein concentrate raw material to 4.5, 33.5 ml of 1 N HCl to adjust to pH 4.75, 28 ml to adjust to pH 5, adjust to pH 5.5 For this purpose, 15 ml of 1N HCl was added. Phytase (Vision Biochem) is added by mixing with the water added to adjust the water content of the composition containing the soy protein concentrate (from 0.005 to 0.05 based on the soy protein concentrate raw material). 0.05% by weight) and reacted at a reaction temperature of 60° C. for 2 to 8 hours to produce a soybean protein concentrate with reduced phytic acid.

In addition, in order to compare the effect of the added phytase concentration, we added 60 wt% water (based on the total composition) and phytase at various concentrations (0.005 wt% to 0.5 wt% based on the weight of the soy protein concentrate raw material). Phytic acid-depleted soy protein concentrates with various contents were prepared by reacting compositions containing soy protein concentrates containing 5% by weight) and adjusted to pH 4.5.

After the reaction was completed, all the samples were dried by freeze-drying and ground to produce a phytic acid-reduced soy protein concentrate in powder form.

[Example 2]
Measurement of phytic acid content and free phosphorus content of soybean protein concentrate with reduced phytic acid The phytic acid analysis kit ( Phytic acid content was measured using the Phytic Acid Assay Kit (Megazyme) according to the manufacturer's manual.

To confirm the phytic acid content of soy protein concentrate according to the content of phytanase and reaction time, it contains water at 60% by weight (based on the total composition) and 0.005% by weight (based on the weight of the raw material). % to 0.5% by weight of a soybean protein concentrate and a pH of 4.5. The phytic acid content (wt%; based on the dry weight of the soybean protein concentrate with reduced phytic acid) is shown in Table 1 below.

In Table 1, 'raw material' refers to the soy protein concentrate raw material used to produce the reduced phytic acid soy protein concentrate, the moisture content of the raw material is approximately 7-8% by weight, and the pH is 6.5 to 6.8 and does not contain phytase. In Table 1 below, 0.005% to 0.5% contained in the composition refers to the content of phytase (% by weight based on the weight of the soy protein concentrate raw material).

As shown in Table 1 above, the composition prepared in Example 1 had a significantly reduced phytic acid content relative to the raw materials.

In addition, a soybean protein concentrate with reduced phytic acid prepared according to the method of Example 1 (phytase at 0.05% by weight based on the weight of the soybean protein concentrate raw material, water at the weight of the entire composition) (manufactured by reacting for 4 hours a composition containing a soy protein concentrate containing 60% by weight based on phytic acid and having a pH of 4.5) and a soy protein concentrate in which phytic acid was not reduced (control group) , free phosphorus content (wt %; on a dry weight basis of the soy protein concentrate with reduced phytic acid), and PO ₄ ^- content (wt %; on a dry weight basis of the soy protein concentrate with reduced phytic acid). ) were measured and are listed in Table 2.

Free phosphorus is calculated based on the absorbance value derived through the megazyme kit analysis process, and this is the phosphorus content released in the original sample without the action of enzymes such as phytase or phosphatase in the phytic acid extract during the kit analysis process. means. The absorbance was measured at 655 nm, and the phosphorus content was calculated by inputting the on-kit free phosphorus absorbance into the total phosphorus absorbance value and calculating the free phosphorus absorbance value as 0. That is, the content of free phosphorus itself was derived by confirming the content of phosphorus calculated from the absorbance of free phosphorus.

PO ₄ ^- is an anionic form of phosphorus that is released when dissolved in a specific solvent, and the resulting value may vary depending on the extraction solvent and method. PO ₄ ^-content was measured using two methods. The room temperature extract is extracted by diluting the sample (composition containing soy protein concentrate with reduced phytic acid) and water at a fixed ratio for 20 minutes at room temperature, and the hot water extract is extracted at room temperature for 20 minutes with boiling water. additionally extracted. The extract was centrifuged, and the supernatant was used as a sample to measure the PO ₄ ^content by ion chromatography.

As shown in Table 2, the soy protein concentrate (control group) contained 0.08 to 0.09 wt% free phosphorus (on a dry matter basis for soy protein concentrate with reduced phytic acid); The PO ₄ ^- content was confirmed to be within 0.2% by weight (based on dry matter of soybean protein concentrate with reduced phytic acid) in all samples collected by two extraction methods, room temperature extraction and hot water extraction. I was able to do that. Free phosphorus refers to the phosphorus content itself, and the PO ₄ ^- content measured through ion chromatography reflects not only the phosphorus content but also the oxygen (O) molecule content. As shown in Table 2, the free phosphorus content of the soybean protein concentrate with reduced phytic acid was about 0.5% by weight, which increased the phosphorus content by about 5-6 times compared to the control group. . In addition, the PO ₄ ^- content of soybean protein concentrate with reduced phytic acid varies slightly depending on the extraction method, but it is around 1.53 to 1.64% by weight, so it is 7 to 8% by weight compared to the control group. The PO ₄ ^-content increased twice.

Therefore, it is confirmed that the soybean protein concentrate with reduced phytic acid produced by the method of Example 1 has a significantly increased content of phosphorus in a form that is easily used in the process of animal feed digestion and absorption. was completed.

Phosphate is an additive used to replenish the phosphorus content in compound feeds, and mono-, di-, and tri-calcium phosphates are often used. Soy protein concentrate with reduced phytic acid increases the phosphorus content in this phosphate form, increasing the nutritional value of other ingredients in addition to protein sources, and making it easier to use phosphorus source additives in whole-feed formulas. It should be possible to provide an economic effect that can reduce the

In addition, the effects of water content and pH of a composition for producing a soybean protein concentrate with reduced phytic acid were confirmed. Therefore, as in the method of Example 1, the soybean protein concentrate raw material is included, the water content is 40 to 60% by weight based on the whole composition, and the phytase is 0.05% by weight based on the soybean protein concentrate raw material. A composition containing a soy protein concentrate containing % by weight and a pH of 4.5 to 6.8 is reacted at 60° C. for 2 to 8 hours, dried and crushed to obtain powdered soybeans with reduced phytic acid. A protein concentrate was prepared, and the free phosphorus content (wt%; based on the dry weight of the soybean protein concentrate with reduced phytic acid) was measured as in the above method and shown in Table 3, and the phytic acid content ( Weight % (based on the dry weight of the soybean protein concentrate with reduced phytic acid) was measured as described above and shown in Table 4.

As shown in Table 3, the composition for producing a reduced phytic acid soy protein concentrate according to an example can increase the free phosphorus content, and the composition for producing a reduced phytic acid soy protein concentrate according to an example It was confirmed that the free phosphorus content in the concentrate increased significantly.

As shown in Table 4 above, the composition for producing a reduced phytic acid soy protein concentrate according to an example can reduce the phytic acid content, and the composition for producing a reduced phytic acid soybean protein concentrate according to an example It was confirmed that the phytic acid content in the protein concentrate was significantly reduced.

[Example 3]
Characteristic analysis of soybean protein concentrate with reduced phytic acid Soybean protein concentrate with reduced phytic acid produced as in the method of Example 1 (phytase of 0.05% based on the soybean protein concentrate raw material) % by weight, water content is 60% by weight based on the total composition, pH is 4.5 to 6.8, and produced by reacting a composition containing soy protein concentrate for 4 hours and then drying) and protein degradation degree (Gel Permeation Chromatography; GPC), protein content (wt%: dry weight basis of soy protein concentrate with reduced phytic acid), phytic acid content (wt%: soy protein with reduced phytic acid). (based on dry weight of the concentrate) was measured and shown in Table 5.

The protein content (wt %) of the phytic acid reduced soy protein concentrate was determined by Kjeldahl instrumental analysis method.

For GPC, approximately 100 mg of a sample (soy protein concentrate with reduced phytic acid) was mixed with 5 ml of 8M urea solution, then subjected to ultrasonic treatment and centrifuged (8000 rpm, 10 minutes), and then the collected supernatant was collected. A sample was analyzed using a GPC column. When deriving analysis results, standard proteins with different molecular weights are analyzed to check the elution delay time (RT), and a standard curve is calculated from the correlation between molecular weight and RT. We were able to derive a distribution map of protein molecular weights. Specifically, the RT at which proteins of less than 30 kDa are detected is measured, and the proportion of proteins of less than 30 kDa contained in the soybean protein concentrate with reduced phytic acid is the RT interval showing less than 30 kDa of the entire chromatogram area. It was calculated based on the area ratio of , and is listed in Table 5. At this time, among the above results, the RT section where the peak of 8M urea extraction solvent appeared was excluded from the entire chromatogram, and then the overall area was calculated.

As shown in Table 5, the soy protein concentrate (control group, raw material) contains approximately 2% by weight (dry weight basis) phytic acid; The phytic acid content of the soybean protein concentrate with reduced phytic acid produced by the method was reduced.

In addition, as a result of GPC analysis, the protein content and the content of peptides less than 30 kDa in the soy protein concentrate with reduced phytic acid were similar to or increased in the soy protein concentrate used as a raw material.

[Example 4]
Specification evaluation of feed containing soybean protein concentrate with reduced phytic acid In this example, soybean protein concentrate with reduced phytic acid (phytase was used as a raw material for soybean protein concentrate) produced as in Example 1 above. After reacting a composition containing soy protein concentrate for 4 hours, the composition contains 0.05% by weight based on the total composition, 60% by weight water based on the total composition, and has a pH of 4.5 to 6.8. The digestibility of fishmeal (prepared by drying) and fishmeal containing no phytic acid was measured.

１３７９．８ｍｇ／ｋｇ；

２２６．４ｍｇ／ｋｇ；Ｆｅ－Ｃｉｔｒａｔｅ、２９９ｍｇ／ｋｇ；ＭｎＳＯ_４、０．０１６ｍｇ／ｋｇ；ＦｅＳＯ_４、０．０３７８ｍｇ／ｋｇ；ＣｕＳＯ_４、０．０００３３ｍｇ／ｋｇ；Calciumiodate、０．０００６；ＭｇＯ、０．００１３５ｍｇ／ｋｇ；ＮａＳｅＯ_３、０．０００２５）。 Specifically, vannamei shrimp (purchased at Paltaksaeu located in Goseong, South Jeolla Province) were preliminarily reared by feeding them with basal feed for 2 weeks, and the breeding experiment was conducted for 4 weeks. Pre-reared vannamei shrimp (average fish weight 14.6 ± 0.34 g) were randomly placed in groups of 10 in 3 replicates, and experimental feed containing the composition listed in Table 6 below was fed 4 times a day to 6 fish of the fish body weight. % and were reared, and feces were collected during rearing. During the experiment, each aquarium was maintained at 29±1° C. using a heater, and the salinity of the breeding water was maintained within the range of 33±1 ppt. The oxygen supply amount was maintained at 6 ppm and the water flow rate was maintained at 0.8 L/min. The experimental feed for the breeding experiment was 6% of the fish body weight four times a day. Fish meal; Anchovy fish meal (Peru), Wheat flour, Soybean meal, Soybean lecithin, Fish oil, Vitamin Mix, Mineral Mix ) was used in feed production. The composition of the vitamin mix used is as follows: (vitamin mix (mg/kg in diet): Ascorbic acid, 300 mg/kg; dl-Calcium pantothenate, 150 mg/kg; Choline bitrate, 3000 mg/kg; Inositol, 150 mg /kg; Menadion, 6mg/kg; Niacin, 150mg/kg; Pyridoxine HCl, 15mg/kg; Riboflavin, 30mg/kg; Thiamine mononitrate, 15mg/kg; dl-a-Tocopherol acetate, 201mg/kg; Retinyl acetate, 6mg /kg; Biotin, 1.5 mg/kg; Folic acid, 5.4 mg/kg; Cobalamin, 0.06). The composition of the mineral mix used is as follows: (mineral mix (mg/kg in diets): NaCl, 437.4 mg/kg;

1379.8mg/kg;

226.4mg/kg; Fe-Citrate, 299mg/kg; MnSO ₄ , 0.016mg/kg; FeSO ₄ , 0.0378mg/kg; CuSO ₄ , 0.00033mg/kg; Calciumiodate, 0.0006; MgO, 0 .00135 mg/kg; NaSeO ₃ , 0.00025).

Feed digestibility was determined by measuring the chromium oxide content, which was used as an indicator, in experimental diets and feces fed to vannamei shrimp. To oxidize chromium oxide (indicator) to the monochromate form, 5-10 mg of the sample (experimental feed or feces) was weighed and transferred to a glass test tube, and 4 ml of perchloric reagent was added. was heated at 300°C for 20 minutes, transferred to a glass flask, added tertiary distilled water to make a sample volume of 25 ml, and measured the absorbance at 350 nm.The phosphorus digestibility (%) of the feed is as follows: The content of phosphorus as a nutrient was determined and calculated using Equation 3, and the results are shown in Table 7 below.

(Formula 3)
Apparant Digestible Coefficient (ADC) = 100 x (1 - (indicator amount in feed/indicator amount in feces) x (nutrient content in feces/nutrient content in feed))

As shown in Table 7, for the feed containing reduced phytic acid soy protein concentrate (DPS) according to an example, the phosphorus digestibility was 80.8%, and the soy protein concentrate with reduced phytic acid In the case of feed containing fishmeal instead of concentrate (FM), the phosphorus digestibility was 81.4%, with no statistically significant difference (p<0.05) between the two feeds, indicating that the phosphorus digestibility was equivalent. I was able to confirm that the level was high. These results indicate that the soybean protein concentrate with reduced phytic acid can be used as a protein feed that can replace fishmeal.

Claims (16)

A composition for producing a soy protein concentrate with reduced phytic acid, the composition comprising:
Contains soy protein concentrate and Phytase
The content of the phytase is 0.005 to 0.5% by weight based on the weight of the soybean protein concentrate;
The water content is 40% by weight or more based on the weight of the entire composition,
pH is 5.5 or less ,
A composition for producing a soybean protein concentrate with reduced phytic acid, wherein the soybean protein concentrate with reduced phytic acid contains 0.4 to 2% by weight of free phosphorus . The composition for producing a soybean protein concentrate with reduced phytic acid according to claim 1, wherein the content of the phytase is 0.025 to 0.5% by weight based on the weight of the soybean protein concentrate. The composition for producing a reduced phytic acid soy protein concentrate according to claim 1 or 2, wherein the water content is 50% by weight or more based on the weight of the entire composition. The composition for producing a soybean protein concentrate with reduced phytic acid according to any one of claims 1 to 3, wherein the pH is 4.5 to 5.5 . A method for producing a soy protein concentrate with reduced phytic acid, comprising the step of reacting a composition according to any one of claims 1 to 4. The method for producing a soybean protein concentrate with reduced phytic acid according to claim 5, further comprising the step of drying the reactant reacted in the step. 7. The method for producing a reduced phytic acid soy protein concentrate according to claim 5 or 6, wherein the reduced phytic acid soy protein concentrate contains less than 1.5% by weight of phytic acid. The production of a soy protein concentrate with reduced phytic acid according to any one of claims 5 to 7, wherein the soy protein concentrate with reduced phytic acid contains 0.4 to 1% by weight of free phosphorus. Method. The phytic acid reduced soy protein concentrate according to any one of claims 5 to 8, wherein the phytic acid reduced soy protein concentrate comprises 15% to 90% of peptides less than 30 kDa based on total peptides. Method for producing soy protein concentrate. Production of a reduced phytic acid soy protein concentrate according to any one of claims 5 to 9, wherein the reduced phytic acid soy protein concentrate is for use in animal feed production. Method. The phytic acid-reduced soybean protein concentrate according to any one of claims 5 to 10, wherein the phytic acid-reduced soybean protein concentrate has increased phosphorus digestibility than the soybean protein concentrate used as a raw material. A method for producing a reduced soy protein concentrate. comprising a soy protein concentrate with reduced phytic acid obtained by reacting a composition according to any one of claims 1 to 4, containing less than 1.5% by weight of phytic acid and containing free phosphorus. A soy protein concentrate with reduced phytic acid containing 0.4 to 2% by weight . 13. The reduced phytic acid soy protein concentrate of claim 12, comprising 0.4 to 1% by weight of free phosphorus. 14. A reduced phytic acid soy protein concentrate according to claim 12 or 13, comprising 15% to 90% of peptides less than 30 kDa based on total peptides. A feed composition comprising a reduced phytic acid soy protein concentrate according to claim 12. The feed composition according to claim 15, further comprising one or more selected from the group consisting of fishmeal, wheat flour, soybean meal, soybean lecithin, fish oil, vitamins, and minerals.