JP7412759B2 - 水溶性食物繊維のエネルギー量の簡便な測定方法 - Google Patents
水溶性食物繊維のエネルギー量の簡便な測定方法 Download PDFInfo
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Description
[1]第一の発明は、D-グルコースを構成単位糖とする水溶性食物繊維のエネルギー換算係数(kcal/g)を求めるための算式を導く方法である。具体的には、
(イ)エネルギー換算係数(y)が既知である前記水溶性食物繊維の2種以上の1H-NMRスペクトルを得る工程と、
(ロ)前記各スペクトルにおいて、4.9~5.7ppmのケミカルシフト範囲(範囲A)にあるシグナル群のピーク面積と、4.4~4.9ppmのケミカルシフト範囲(範囲B)にあるシグナル群のピーク面積との合計面積における、範囲Bにあるシグナル群のピーク面積比(x)を算出する工程と、
(ハ)前記yとxの関係式(但し、ピアソンの相関係数rが-0.7から-1.0)を導く工程とを含む、方法である。
[2]第二の発明は、D-グルコースを構成単位糖とする水溶性食物繊維のエネルギー換算係数(kcal/g)を求める方法である。具体的には、
(ニ)前記水溶性食物繊維の1H-NMRスペクトルを得る工程と、
(ホ)前記各スペクトルにおいて、4.9~5.7ppmのケミカルシフト範囲(範囲A)にあるシグナル群のピーク面積と、4.4~4.9ppmのケミカルシフト範囲(範囲B)にあるシグナル群のピーク面積との合計面積における、範囲Bにあるシグナル群のピーク面積比(x)を算出する工程と、
(ヘ)前記xを前記1記載の方法により導出された算式に代入し、エネルギー換算係数(y)を求める工程とを含む、方法である。
[3]第三の発明は、前記[2]の方法において算式が以下である方法である。
数式: y=-0.0307x+2.1872(kcal/g)。 但し、0≦y≦2、かつ、x<71.244とする。
各試料10mgを重水0.6mLに溶解して試料溶液とした(なお、内部基準物質としてトリメチルシリルプロパン酸ナトリウム(TSP)を微量添加しておき、このTSP由来のスペクトルのピークを0ppmとした)。
試料溶液を外径5mmのNMR試料管に入れ、以下の条件の1H-NMR法で測定した。
装置:JNM-ECA500(日本電子株式会社製)
1H共鳴周波数:500MHz
測定温度:80℃
積算回数:32
上記の測定条件によって得られる1H-NMRスペクトルの一例として、「ニュートリオースFM06」(ロケット社製)のスペクトルを図1に示す。水溶性食物繊維の構成単位糖のD-グルコースの1位の水素原子のうち、α結合しているものは約4.9~5.7ppmのケミカルシフト範囲(範囲A)に、β結合しているものは約4.4~4.9ppmのケミカルシフト範囲(範囲B)に帰属され、D-グルコースの2~6位の水素原子は、約3.2~4.1ppmのケミカルシフト範囲に帰属されると推定されるため、範囲A及び範囲Bにあるシグナル群のピーク面積における、範囲Bにあるシグナル群のピーク面積の割合を、解析ソフト「Delta5.3.0」(日本電子株式会社製)を用いて算出し、「β結合の割合」とした。なお、「Delta5.3.0」を用いた解析では、「範囲A及び範囲Bにあるシグナル群のピーク面積」の積分値を100としたときの「範囲Bのピーク面積」の積分値比が、「β結合の割合」として算出されることとなる。
Claims (3)
- D-グルコースを構成単位糖とする水溶性食物繊維のエネルギー換算係数(kcal/g)を求めるための算式を導出する方法であって、
(イ)エネルギー換算係数(y)が既知である前記水溶性食物繊維の2種以上の1H-NMRスペクトルを得る工程と、
(ロ)前記各スペクトルにおいて、4.9~5.7ppmのケミカルシフト範囲(範囲A)にあるシグナル群のピーク面積と、4.4~4.9ppmのケミカルシフト範囲(範囲B)にあるシグナル群のピーク面積との合計面積における、範囲Bにあるシグナル群のピーク面積比(x)を算出する工程と、
(ハ)前記yとxの関係式(但し、ピアソンの相関係数rが-0.7から-1.0)を導く工程とを含む、方法。
- D-グルコースを構成単位糖とする水溶性食物繊維のエネルギー換算係数(kcal/g)を求める方法であって、
(ニ)前記水溶性食物繊維の1H-NMRスペクトルを得る工程と、
(ホ)工程(二)で得られたスペクトルにおいて、4.9~5.7ppmのケミカルシフト範囲(範囲A)にあるシグナル群のピーク面積と、4.4~4.9ppmのケミカルシフト範囲(範囲B)にあるシグナル群のピーク面積との合計面積における、範囲Bにあるシグナル群のピーク面積比(x)を算出する工程と、
(ヘ)前記xを請求項1記載の方法により導出された算式に代入し、エネルギー換算係数(y)を求める工程とを含む、方法。
- D-グルコースを構成単位糖とする水溶性食物繊維のエネルギー換算係数(kcal/g)を求める方法であって、算式が以下である、請求項2記載の方法。
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2008297211A (ja) | 2007-05-29 | 2008-12-11 | Matsutani Chem Ind Ltd | オリゴ糖、その製造方法、及びその用途 |
JP2017074072A (ja) | 2010-03-10 | 2017-04-20 | イノボバイオロジック, インコーポレイテッド | グルコマンナン、キサンタンガム、およびアルギネートを含む、代謝性障害の処置のための食品 |
US20170360303A1 (en) | 2016-06-16 | 2017-12-21 | Samsung Electronics Co., Ltd. | Calorie estimation apparatus and method, and wearable device |
US20190264254A1 (en) | 2016-03-24 | 2019-08-29 | California State University, Fresno | Method of measuring the deleterious effects of non-caloric artificial sweeteners on enzymatic hydrolysis of sucrose measured in real time |
WO2020051716A1 (en) | 2018-09-14 | 2020-03-19 | 10250929 Canada Inc. | Method and system for in-vivo, and non-invasive measurement of metabolite levels |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008297211A (ja) | 2007-05-29 | 2008-12-11 | Matsutani Chem Ind Ltd | オリゴ糖、その製造方法、及びその用途 |
JP2017074072A (ja) | 2010-03-10 | 2017-04-20 | イノボバイオロジック, インコーポレイテッド | グルコマンナン、キサンタンガム、およびアルギネートを含む、代謝性障害の処置のための食品 |
US20190264254A1 (en) | 2016-03-24 | 2019-08-29 | California State University, Fresno | Method of measuring the deleterious effects of non-caloric artificial sweeteners on enzymatic hydrolysis of sucrose measured in real time |
US20170360303A1 (en) | 2016-06-16 | 2017-12-21 | Samsung Electronics Co., Ltd. | Calorie estimation apparatus and method, and wearable device |
WO2020051716A1 (en) | 2018-09-14 | 2020-03-19 | 10250929 Canada Inc. | Method and system for in-vivo, and non-invasive measurement of metabolite levels |
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