JP7387183B2 - 牛の肉質の軟化方法 - Google Patents
牛の肉質の軟化方法 Download PDFInfo
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- 150000001875 compounds Chemical class 0.000 claims description 9
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 23
- 235000017471 coenzyme Q10 Nutrition 0.000 description 16
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 13
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 13
- 210000003205 muscle Anatomy 0.000 description 12
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 12
- 238000005259 measurement Methods 0.000 description 11
- 102000007590 Calpain Human genes 0.000 description 8
- 108010032088 Calpain Proteins 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
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- 210000000115 thoracic cavity Anatomy 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 229940087168 alpha tocopherol Drugs 0.000 description 6
- 238000010411 cooking Methods 0.000 description 6
- 238000003307 slaughter Methods 0.000 description 6
- 229960000984 tocofersolan Drugs 0.000 description 6
- 239000002076 α-tocopherol Substances 0.000 description 6
- 235000004835 α-tocopherol Nutrition 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 235000013325 dietary fiber Nutrition 0.000 description 5
- 230000001953 sensory effect Effects 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 210000000481 breast Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000003149 assay kit Methods 0.000 description 3
- 235000019784 crude fat Nutrition 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
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- 210000000876 intercostal muscle Anatomy 0.000 description 2
- VOEYXMAFNDNNED-UHFFFAOYSA-N metolcarb Chemical compound CNC(=O)OC1=CC=CC(C)=C1 VOEYXMAFNDNNED-UHFFFAOYSA-N 0.000 description 2
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- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 230000009469 supplementation Effects 0.000 description 2
- RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 1
- 238000009020 BCA Protein Assay Kit Methods 0.000 description 1
- 102000010831 Cytoskeletal Proteins Human genes 0.000 description 1
- 108010037414 Cytoskeletal Proteins Proteins 0.000 description 1
- 241000234642 Festuca Species 0.000 description 1
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000287462 Phalacrocorax carbo Species 0.000 description 1
- 239000012083 RIPA buffer Substances 0.000 description 1
- 239000000524 Thiobarbituric Acid Reactive Substance Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229940110767 coenzyme Q10 Drugs 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
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- 229940118019 malondialdehyde Drugs 0.000 description 1
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- 238000012545 processing Methods 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Description
(1) 牛に還元型コエンザイムQ10を含む飼料を給与することを含み、前記飼料中の配合飼料の割合が、80重量%~90重量%である、牛の肉質の軟化方法。
(2) 前記還元型コエンザイムQ10の給与量が、0.1~10g/頭/日である、(1)記載の方法。
(3) 前記牛が黒毛和種である(1)又は(2)に記載の方法。
(4) 牛に還元型コエンザイムQ10を含む飼料を給与することを含み、前記飼料中の配合飼料の割合が、80重量%~90重量%である、牛肉の食味改善方法。
(5) 前記還元型コエンザイムQ10の給与量が、0.1~10g/頭/日である、(4)記載の方法。
(6) 少なくとも出荷前の3ヶ月間にわたり、前記還元型コエンザイムQ10を給与する、(4)又は(5)記載の方法。
約155ヶ月齢の黒毛和種経産牛23頭を用いた。JA東日本くみあい飼料株式会社製造の経産牛肥育用配合飼料「39かあさん」(表1)を給与した。粗飼料はフェスクストローを給与した。廃用牛として判断してから3週間の馴致後、約6ヶ月間飼養した。配合飼料の量を制限し、CoQ10を給与しない群を対照区(n=6)(比較例1)、配合飼料の量を多くし、CoQ10を給与しない群を肥育区(n=6)(比較例2)、対照区に加えてCoQ10を1g/頭/日給与する群をCoQ区(n=6)(実施例1)、肥育区に加えてCoQ10を1g/頭/日給与する群をCoQ肥育区(n=6)(実施例2)とした。なお、対照区と肥育区の給与体系は表2のとおりとした。その他の飼養管理は常法に従った。
胸最長筋を厚さ1.8cmにカットした後、中心部が70℃に達するまで70℃に設定したウォーターバスで加熱し、オーバーナイトで冷蔵後、インストロン5542型によりせん断力価を測定した。筋線維に沿って1.27cmにくりぬいた肉片を、筋線維に垂直にV字ブレードでせん断した際の最大点圧縮荷重を測定し、せん断力価とした。せん断力価測定用アタッチメントであるV字ブレードのせん断速度は250 mm/分とした。
Calpain Assay Kit(Promo Cell社製)を用いて分析した。結果は対照区に対する相対値として示した。
70℃に設定したウォーターバスで胸最長筋を60分間加熱し肉の中心温度を70℃にし、加熱前後の重量差から計算した。
3gの胸最長筋を50ml遠沈管に採取し、これに1% NaCl溶液を1ml加えて懸濁後、さらに3%ピロガロール・エタノール溶液を10mlおよび60%KOHを2ml加えて70℃ で30分間ケン化した。遠沈管を氷水に浸けて反応を停止し、これに1% NaCl溶液14ml、酢酸エチル・n-ヘキサン混液(1:9)14mlを加えて、5分間激しく振とうした。1500rpmで5分間遠心分離し、上層のヘキサン層を100ml吸引採取した。これに酢酸エチル・n-ヘキサン混液(1:9)14mlを加えて再溶解した。振とう抽出・遠心分離・吸引採取を2回繰り返し、N2ガスで乾固した。HPLC用ヘキサンで希釈し、クロマトディスク0.45μm通して、HPLC分析した。HPLCは日本分光株式会社製(ポンプPU-2080Plus,蛍光検出器FP-2020Plus)を用いた.カラムは株式会社ワイエムシィ製YMC-Pack SIL-06 (内径 4.6mm×250mm)を用い、移動層はヘキサン、酢酸およびイソプロパノール(1000:5:6)で流量は1.5ml/分であった。また、検出波長は298nm(EX)および325nm(EM)であった。
血清:OXI-TEK TBARS-Assay kit(Alexis Corporation、USA、New York)を用い、製造者のマニュアルに従って、測定した。胸最長筋:約0.1gの胸最長筋に500μlのRIPAバッファーを加え、氷冷下でホモジナイズしたサンプルを用い、OXI-TEK TBARS-Assay kit(Alexis Corporation、USA、New York)により、製造者のマニュアルに従って、測定した。得られたマロンジアルデヒド量は総タンパク量で補正した。総タンパク質含量の測定にはBCA Protein Assay Kit(Thermo Scientific)を用いた。
約27ヶ月齢の黒毛和種去勢肥育牛10頭および黒毛和種雌肥育牛10頭を供試した。1頭1日当り、JA東日本くみあい飼料社製の肥育用配合飼料「うし王後期」を10 kgと、粗飼料として稲わらを2.0kg給与した。30ヶ月齢まで肥育後、出荷、屠殺し、得られた肉を調査した。
ア.対照区(去勢)(n=5);当室の慣行に従い飼育した。
イ.CoQ区(去勢)(n=5);対照区に加えて、3ヶ月間CoQ10を1g/頭/日給与した。
ウ.対照区(雌)(n=5);当室の慣行に従い飼育した。
エ.CoQ区(雌)(n=5);対照区に加えて、3ヶ月間CoQ10を1g/頭/日給与した。
Claims (6)
- 牛に還元型コエンザイムQ10を含む飼料を給与することを含み、前記飼料中の配合飼料の割合が、80重量%~90重量%である、牛の肉質の軟化方法。
- 前記還元型コエンザイムQ10の給与量が、0.1~10g/頭/日である、請求項1記載の方法。
- 前記牛が黒毛和種である請求項1又は2に記載の方法。
- 牛に還元型コエンザイムQ10を含む飼料を給与することを含み、前記飼料中の配合飼料の割合が、80重量%~90重量%である、牛肉の食味改善方法。
- 前記還元型コエンザイムQ10の給与量が、0.1~10g/頭/日である、請求項4記載の方法。
- 少なくとも出荷前の3ヶ月間にわたり、前記還元型コエンザイムQ10を給与する、請求項4又は5記載の方法。
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JP2021033748A JP7387183B2 (ja) | 2021-03-03 | 2021-03-03 | 牛の肉質の軟化方法 |
JP2022175530A JP2023002808A (ja) | 2021-03-03 | 2022-11-01 | 牛の肉質の軟化方法 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003102328A (ja) | 2001-09-28 | 2003-04-08 | Katsuya Kato | 肉牛への給飼方法および肉牛用飼料 |
WO2008156108A1 (ja) | 2007-06-21 | 2008-12-24 | Kaneka Corporation | 機能性家畜生産品およびその製造方法 |
WO2011081208A1 (ja) | 2009-12-28 | 2011-07-07 | 株式会社カネカ | ビタミンe濃度が高められた豚肉およびその製造方法とそのための飼料 |
JP2011182748A (ja) | 2010-03-10 | 2011-09-22 | Hyogo Prefecture | 家畜又は家禽用飼料 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2008005809A (ja) * | 2006-06-30 | 2008-01-17 | Natl Fedelation Of Agricult Coop Assoc | 肉牛用飼料及びその給与方法 |
JP6839392B2 (ja) * | 2018-10-29 | 2021-03-10 | 全国農業協同組合連合会 | 小麦わら成形飼料およびこれを用いた家畜の飼育方法 |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003102328A (ja) | 2001-09-28 | 2003-04-08 | Katsuya Kato | 肉牛への給飼方法および肉牛用飼料 |
WO2008156108A1 (ja) | 2007-06-21 | 2008-12-24 | Kaneka Corporation | 機能性家畜生産品およびその製造方法 |
WO2011081208A1 (ja) | 2009-12-28 | 2011-07-07 | 株式会社カネカ | ビタミンe濃度が高められた豚肉およびその製造方法とそのための飼料 |
JP2011182748A (ja) | 2010-03-10 | 2011-09-22 | Hyogo Prefecture | 家畜又は家禽用飼料 |
Non-Patent Citations (5)
Title |
---|
6章 産肉生理と枝肉評価,新 黒毛和種肥育の手引(平成18年3月),日本,北海道立畜産試験場,第46-55頁,https://www.hro.or.jp/list/agricultural/research/sintoku/common/publication/feedmanual/feedman16.pdf |
三津本充 外6名,黒毛和種去勢牛への屠殺前4週間のビタミンE投与による展示中の牛肉色と脂質の安定化,日本畜産学会報(1995),第66巻第11号,日本,第962-968頁,https://www.jstage.jst.go.jp/article/chikusan1924/66/11/66_11_962/_pdf/-char/ja |
堀井美那 外2名,黒毛和種去勢牛の短期肥育における前期粗飼料給与水準が発育および肉質に及ぼす影響,栃木畜試研報(2006) ,第22号,日本,第41-46頁,http://210.164.7.60/g70/haga/system/desaki/desaki/documents/22-7.pdf |
庄司則章,黒毛和種雌肥育午へのビタミンE製剤の給与効果,山形県農業研究報告(2010年3月),第2号,日本,山形県農業総合研究センター,第79-83頁,https://agriknowledge.affrc.go.jp/RN/2010792721.pdf |
松本光人,肉用牛肥育における飼育管理について,講演資料,日本,畜産草地研究所,2011年8月3日,http://www.anan-zaidan.or.jp/lecture/4.matumoto.pdf |
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