JP7377400B1 - Intelligent monitoring and sampling equipment and sampling method of planktonic organisms in seagrass bed ecosystem - Google Patents

Intelligent monitoring and sampling equipment and sampling method of planktonic organisms in seagrass bed ecosystem Download PDF

Info

Publication number
JP7377400B1
JP7377400B1 JP2023119532A JP2023119532A JP7377400B1 JP 7377400 B1 JP7377400 B1 JP 7377400B1 JP 2023119532 A JP2023119532 A JP 2023119532A JP 2023119532 A JP2023119532 A JP 2023119532A JP 7377400 B1 JP7377400 B1 JP 7377400B1
Authority
JP
Japan
Prior art keywords
collection
sampling
mounting
filtration membrane
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023119532A
Other languages
Japanese (ja)
Inventor
陳▲つぇん▼
彭勃
王敏
汪元南
呉艶麗
黄基霖
賀涛
Original Assignee
生態環境部華南環境科学研究所(生態環境部生態環境応急研究所)
曁南大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 生態環境部華南環境科学研究所(生態環境部生態環境応急研究所), 曁南大学 filed Critical 生態環境部華南環境科学研究所(生態環境部生態環境応急研究所)
Application granted granted Critical
Publication of JP7377400B1 publication Critical patent/JP7377400B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

【課題】海草藻場生態系における浮遊生物のインテリジェント監視およびサンプリング装置およびサンプリング方法であり、水域環境サンプリング装置の技術分野に属する。【解決手段】サンプリング装置は、サンプリング本体、インテリジェント収集部品、駆動部品およびインテリジェント制御端末を備え、本発明の装置は、収集トレイの収集深度を調整するのに便利であるだけでなく、異なる水域懸濁物を勾配で収集し、次に海草藻場中の濃縮した水域懸濁物DNAを採用し、収集したサンプルがより完全で、検出したデータがより正確である。【選択図】図1The present invention provides an intelligent monitoring and sampling device and sampling method for floating organisms in a seagrass bed ecosystem, which belongs to the technical field of aquatic environment sampling device. The sampling device includes a sampling body, an intelligent collection part, a driving part and an intelligent control terminal, and the device of the present invention is not only convenient to adjust the collection depth of the collection tray, but also suitable for different water body suspensions. The turbidity is collected in a gradient, and then the concentrated aquatic suspension DNA in the seagrass bed is adopted, the collected samples are more complete and the detected data are more accurate. [Selection diagram] Figure 1

Description

本発明は、水域環境サンプリング装置の技術分野に属し、具体的に、海草藻場生態系にお
ける浮遊生物のインテリジェント監視およびサンプリング装置およびサンプリング方法に
関する。
The present invention belongs to the technical field of aquatic environment sampling device, and specifically relates to an intelligent monitoring and sampling device and sampling method for planktonic organisms in seagrass bed ecosystem.

近年、地球規模の気候変動の影響が顕在化し、「カーボンピーキング」や「カーボンニュ
ートラル」が徐々に世界的なコンセンサスになって、地球上で最大の炭素貯蔵庫である海
洋は、グローバルな気候ガバナンスにおいて基本的な役割を担っている。中国の海洋戦略
と「ダブルカーボン」目標の背景において、海洋炭素吸収源(ブルーカーボン)の役割と
意義はますます顕著になってきている。
ブルーカーボンとは、海洋生物を利用して大気中の二酸化炭素を吸収し、海洋に固定する
プロセス、活動、メカニズムを指し、グリーンカーボンシンクなど他の炭素吸収源と比較
して、ブルーカーボンは炭素吸収量が大きく、効率が高く、貯蔵期間が長いという特徴が
ある。森林や草原などの陸上生態系は、貯蔵サイクルが最長でも数十年しかないのに対し
、ブルーカーボンの吸収源は数百年、数千年と続き、大きな炭素吸収効果を発揮する。し
たがって、ブルーカーボンは温室効果ガスの排出を効果的に緩和し、カーボンニュートラ
ルという目標の達成に貢献することができ、グローバルな気候ガバナンスのフロンティア
領域となっている。
沿岸の3大ブルーカーボン生態系の一つであり、海洋生物の生息地や重要な食物連鎖とし
て機能し、沖合の基質や海岸線を安定化させる役割を果たす。海草藻場は微生物群集が豊
富で、沖合の物質循環や生物地球化学プロセスにおいて重要な役割を果たし、生態系の安
定を維持するために不可欠であり、海草微生物群集の組成は、海草の生態状態を効果的に
把握するための初期指標として、また海草生態系が直面する環境ストレスをより包括的か
つタイムリーに理解するために、海草藻場内のバイオモニタリングに重要である。
既存の海草藻場生態系中の生物のインテリジェント監視およびサンプリング装置は、水域
中のすべての水域懸濁物を統一に濃縮し、階層的収集機能がなく、遠心分離プロセスがよ
り困難になっている。
In recent years, the effects of global climate change have become apparent, and "carbon peaking" and "carbon neutrality" have gradually become a global consensus, and the ocean, which is the largest carbon storage on the planet, has become an important part of global climate governance. plays a fundamental role. In the context of China's ocean strategy and "double carbon" goal, the role and significance of ocean carbon sinks (blue carbon) are becoming increasingly prominent.
Blue carbon refers to the processes, activities, and mechanisms that use marine organisms to absorb carbon dioxide from the atmosphere and fix it in the ocean. Compared to other carbon sinks, such as green carbon sinks, blue carbon It has the characteristics of large absorption capacity, high efficiency, and long storage period. While terrestrial ecosystems such as forests and grasslands have a storage cycle of only a few decades at most, blue carbon sinks last for hundreds or thousands of years, exerting a large carbon absorption effect. Therefore, blue carbon can effectively mitigate greenhouse gas emissions and contribute to achieving the goal of carbon neutrality, making it a frontier area of global climate governance.
It is one of the three major coastal blue carbon ecosystems, serving as habitat for marine life and an important food chain, and plays a role in stabilizing offshore substrates and shorelines. Seagrass beds are rich in microbial communities, play important roles in offshore material cycles and biogeochemical processes, and are essential for maintaining ecosystem stability, and the composition of seagrass microbial communities depends on the ecological status of seagrasses. It is important for biomonitoring within seagrass beds, as an early indicator to effectively understand the environmental stressors facing seagrass ecosystems, and for a more comprehensive and timely understanding of the environmental stresses faced by seagrass ecosystems.
The existing intelligent monitoring and sampling equipment of living organisms in the seagrass bed ecosystem uniformly concentrates all aquatic suspensions in the water body, and there is no hierarchical collection function, making the centrifugation process more difficult. .

上記の問題に解決するために、本発明は、海草藻場生態系における浮遊生物のインテリジ
ェント監視およびサンプリング装置およびサンプリング方法を提供する。
本発明の技術的解決策は以下の通りである。海草藻場生態系における浮遊生物のインテリ
ジェント監視およびサンプリング装置は、中心に外部と連通する取付調節口が設けられた
サンプリング本体、前記取付調節口に設けられたインテリジェント収集部品、前記サンプ
リング本体の上端に設けられて前記インテリジェント収集部品に接続された駆動部品およ
びインテリジェント制御端末を備え、
前記インテリジェント収集部品は、下端の左右両側に収集サブボックスが設けられた収集
メインボックス、前記収集メインボックス内に設けられた第1濾過膜、前記収集サブボッ
クス内に設けられた第2濾過膜、エ字形の収集ヘッドおよび前記エ字形の収集ヘッドに接
続された水ポンプを含み、前記エ字形の収集ヘッドは、上端が収集メインボックスの下端
を貫通してその内部に延伸する垂直収集管、収集メインボックス内に設けられて垂直収集
管の上端と連通する噴霧ディスク、垂直収集管の下端に接続された収集トレイを含み、前
記噴霧ディスクの下端に複数の噴霧口が均一に設けられ、前記収集トレイの下端に複数の
収集孔が均一に設けられ、垂直収集管に水流量センサーが設けられ、収集サブボックスの
高さ、エ字形の収集ヘッドの長さは必要に応じて調整可能であり、水流量センサーはエ字
形の収集ヘッドに入った水量を検出するために使用され、
前記第1濾過膜と第2濾過膜はいずれも着脱可能な構造であり、第1濾過膜は垂直収集管
の外壁に嵌設された取付内輪、前記取付内輪と同軸に設けられた取付外輪、取付内輪と前
記取付外輪間に設けられた複数の分離ロッドおよび隣接する2つの前記分離ロッド間に可
動に設けられたサブ濾過膜を含み、第2濾過膜はシリコン膜およびシリコン膜を固定する
ための外枠を含み、
前記駆動部品は、サンプリング本体の上端に設けられたH字形の取付ラック、前記H字形
の取付ラックの水平セグメントに設けられた回転ローラ、前記回転ローラを回転させるた
めの駆動モータを含み、回転ローラの外壁に収集メインボックスを接続するための巻きロ
ープが巻かれ、
前記インテリジェント制御端末は、水ポンプと水流量センサーに電気的に接続されたコン
トローラ、サンプリング本体に設けられたGPSモジュール、サンプリング本体に設けら
れて遠隔信号転送に用いられる無線通信モジュール、および故障警報モジュールを含む。
本発明の一側面によれば、前記サンプリング本体の下端に転倒防止部品が設けられ、前記
転倒防止部品は、サンプリング本体の下端に垂直に設けられた取付摺動板、垂直セグメン
トが前記取付摺動板の中心に可動に接続された逆T字形の固定柱、前記逆T字形の固定柱
の左右両側に対称に分布されて上端が取付摺動板に摺動可能に接続された平衡摺動板、逆
T字形の固定柱の垂直セグメントの外部に嵌設されて外壁が周方向に沿って均一に設けら
れた複数のブレードを有する摺動スリーブを含み、前記摺動スリーブと逆T字形の固定柱
の水平セグメント間に複数の反発バネが均一に設けられ、摺動スリーブと各平衡摺動板間
に調節摺動ロッドがヒンジで接続され、サンプルを収取するとき、風速が大きすぎると、
風の流れが各ブレードに垂直に吹き付け、摺動スリーブを取付摺動板に近い側方に移動さ
せるように駆動し、このとき、摺動スリーブは各反発バネを圧縮し、調節摺動ロッドは、
対応の平衡摺動板を押してそれぞれ取付摺動板の左右両側に沿って摺動させ、平衡摺動板
がそれぞれサンプリング本体の左右両側に位置し、サンプリング本体の平衡を取る目的を
達成し、サンプリング本体の転倒を防止し、サンプリング本体の走行安定性を向上させ、
プロセス全体で外部の駆動部材を必要とせず、環境保護・省エネという利点を有し、本装
置を使用しないとき、2つの平衡摺動板が互いに近接してサンプリング本体の下端に収容
されるため、省スペース化が実現される。
本発明の一側面によれば、前記取付摺動板の左右両側にそれぞれ制限遮断ブロックが設け
られ、前記平衡摺動板は、取付摺動板に摺動可能に接続された摺動板、前記摺動板の下方
に設けられ長さが摺動板の長さよりも大きい平衡板を含み、前記平衡板は中空構造であり
、中空構造の平衡板内に発泡材が充填され、摺動板が取付摺動板上で左右に摺動すること
により、平衡板の距離を調節し、制限遮断ブロックの設置により、摺動板と取付摺動板の
離脱による転倒防止部品の通常使用に悪影響を与えることを回避する。
本発明の一側面によれば、前記収集トレイの下端にスリーブが設けられ、前記スリーブの
下端に電動破砕ローラが設けられ、スリーブ内であって前記電動破砕ローラの上端にフィ
ルターが設けられ、電動破砕ローラにより水域中の植物を破砕し、フィルターで水域中の
微細な固体不純物を濾過し、水域中の固体不純物と植物が収集トレイ内に入って、閉塞す
ることにより通常の収集作業に悪影響を与えることを回避する。
本発明の一側面によれば、前記サンプリング本体の側壁に衝突防止部品が設けられ、前記
衝突防止部品は、複数の第1バネ接続柱を介してサンプリング本体の側壁に接続された水
平湾曲緩衝板、第2バネ接続柱を介して前記水平湾曲緩衝板に接続された複数の垂直湾曲
緩衝板を含み、単層構造の緩衝板と比較すると、本装置は、水平湾曲緩衝板と垂直湾曲緩
衝板で2重緩衝構造を形成し、より良好な緩衝能力を有し、緩衝装置の使用寿命を延長す
ることができる。
本発明の一側面によれば、各前記垂直湾曲緩衝板の側壁にそれぞれ緩衝エアバッグが設け
られ、外力が垂直湾曲緩衝板に直接に当たって垂直湾曲緩衝板が損傷することを回避する
ことができる。
本発明の一側面によれば、前記第1濾過膜の孔径は0.45μmであり、前記第2濾過膜
の孔径は0.2μmであり、第1濾過膜を通じて水域中の真核生物を濃縮し、第2濾過膜
を通じて水域中の細菌を濃縮し、その後濃縮した水域懸濁物DNAを採用し、収集したサ
ンプルがより完全で、検出したデータがより正確である。
本発明は、上記海草藻場生態系における浮遊生物のインテリジェント監視およびサンプリ
ング装置に基づく、海草藻場生態系における浮遊生物のインテリジェント監視およびサン
プリング方法をさらに開示し、この方法は、
S1、コントローラを通じて外部駆動装置を制御してサンプリング本体を指定の水環境領
域に移動させ、駆動モータを起動し、駆動モータを通じて回転ローラを時計回りに回転さ
せるように駆動し、回転ローラから巻きロープが連続的に巻き下げられ、このとき、収集
メインボックスの重力作用下で、その両側の収集サブボックスが取付調節口内で下方に摺
動し、収集トレイが同期して水域に移動し、収集トレイが予設した収集深度に達すると、
コントローラを通じて外部駆動装置を閉じるように制御するステップと、
S2、水ポンプを起動し、水が各収集孔を通って収集トレイに流入し、垂直収集管を通っ
て噴霧ディスク内に入り、噴霧口を通って第1濾過膜上に均一に噴霧し、第1濾過膜を通
じて水環境中の真核生物を濃縮し、水が第1濾過膜を通って流下し、逆T字形の固定柱の
両側からそれぞれ収集サブボックス内に落下し、その後、第2濾過膜を通じて水環境中の
細菌を濃縮し、水が第2濾過膜を通って水環境に流れ、水流量センサーにより逆T字形の
固定柱に入った水量を検出し、予設水量に達すると、海草藻場中の微生物DNAの収集を
完了するステップと、
S3、サンプルを収集するとき、電動破砕ローラを通じて水域中の植物を破砕し、フィル
ターを通じて水域中の微細な固体不純物を濾過し、水平湾曲緩衝板と垂直湾曲緩衝板で2
重緩衝構造を形成して、サンプリング本体に衝突する外力を遮断するステップと、
S4、サンプルを収集するとき、風の流れが各ブレードに垂直に吹き付け、摺動スリーブ
を取付摺動板に近い側方に移動させるように駆動し、このとき、摺動スリーブは各反発バ
ネを圧縮し、調節摺動ロッドは、対応の平衡摺動板を押してそれぞれ取付摺動板の左右両
側に沿って摺動させ、平衡摺動板がそれぞれサンプリング本体の左右両側に位置し、サン
プリング本体の平衡を取るステップと、を含む。
To solve the above problems, the present invention provides an intelligent monitoring and sampling device and sampling method for planktonic organisms in a seagrass bed ecosystem.
The technical solution of the present invention is as follows. An intelligent monitoring and sampling device for floating organisms in a seagrass bed ecosystem includes a sampling body provided with an installation control port communicating with the outside at the center, an intelligent collection part installed in the installation control port, and an intelligent collection part installed at the top of the sampling main body. a driving component and an intelligent control terminal provided and connected to the intelligent collecting component;
The intelligent collection component includes a collection main box having collection sub-boxes on both left and right sides of the lower end, a first filtration membrane installed in the collection main box, and a second filtration membrane installed in the collection sub-box. an E-shaped collection head and a water pump connected to the E-shaped collection head, the E-shaped collection head comprising a vertical collection pipe whose upper end extends through the lower end of the collection main box and into the collection main box; a spray disc installed in the main box and communicating with the upper end of the vertical collection pipe; a collection tray connected to the lower end of the vertical collection pipe; a plurality of spray ports are uniformly provided at the lower end of the spray disc; Multiple collecting holes are uniformly provided at the lower end of the tray, and a water flow sensor is provided in the vertical collecting pipe, and the height of the collecting sub-box and the length of the E-shaped collecting head can be adjusted as required; The water flow sensor is used to detect the amount of water entering the E-shaped collection head,
Both the first filtration membrane and the second filtration membrane have a removable structure, and the first filtration membrane includes an inner mounting ring fitted to the outer wall of the vertical collection pipe, an outer mounting ring coaxially with the inner mounting ring, A second filtration membrane includes a plurality of separation rods provided between the inner mounting ring and the outer mounting ring and a sub-filtration membrane movably provided between the two adjacent separation rods, and the second filtration membrane is for fixing a silicone membrane and a silicone membrane. including the outer frame of
The driving parts include an H-shaped mounting rack provided at the upper end of the sampling body, a rotating roller provided on a horizontal segment of the H-shaped mounting rack, and a drive motor for rotating the rotating roller, and the rotating roller A winding rope is wound to connect the main collection box to the outside wall of the
The intelligent control terminal includes a controller electrically connected to the water pump and the water flow rate sensor, a GPS module installed in the sampling body, a wireless communication module installed in the sampling body and used for remote signal transmission, and a fault alarm module. including.
According to one aspect of the present invention, an overturn prevention component is provided at the lower end of the sampling body, and the overturn prevention component includes a mounting sliding plate provided perpendicularly to the lower end of the sampling body, and a vertical segment of the mounting sliding plate provided vertically at the lower end of the sampling body. an inverted T-shaped fixed column movably connected to the center of the plate; a balanced sliding plate distributed symmetrically on both left and right sides of the inverted T-shaped fixed column and whose upper end is slidably connected to the mounting sliding plate; , a sliding sleeve fitted on the outside of the vertical segment of the inverted T-shaped fixing column and having an outer wall having a plurality of blades uniformly provided along the circumferential direction, the sliding sleeve and the inverted T-shaped fixing A plurality of rebound springs are uniformly provided between the horizontal segments of the column, and an adjustment sliding rod is hinged between the sliding sleeve and each counterbalance sliding plate, when collecting the sample, when the wind speed is too large,
A stream of wind blows perpendicularly to each blade, driving the sliding sleeve to move sideways closer to the mounting sliding plate, at which time the sliding sleeve compresses each rebound spring, and the adjusting sliding rod ,
Push the corresponding balancing sliding plates and sliding them along the left and right sides of the mounting sliding plate, respectively, so that the balancing sliding plates are located on the left and right sides of the sampling body, respectively, to achieve the purpose of balancing the sampling body, and the sampling Prevents the main body from falling and improves the running stability of the sampling main body.
The whole process does not require external driving members, which has the advantages of environmental protection and energy saving, and when the device is not in use, the two balance sliding plates are housed in the lower end of the sampling body in close proximity to each other; Space saving is realized.
According to one aspect of the present invention, restriction blocking blocks are provided on both left and right sides of the mounting sliding plate, and the balancing sliding plate includes a sliding plate slidably connected to the mounting sliding plate, and a The balance plate is provided below the sliding plate and has a length larger than the length of the sliding plate, and the balancing plate has a hollow structure, and the hollow structure of the balancing plate is filled with a foam material, and the sliding plate is By sliding left and right on the mounting sliding plate, the distance of the balance plate can be adjusted, and the installation of a limit blocking block will adversely affect the normal use of the anti-falling parts due to the separation of the sliding plate and the mounting sliding plate. avoid that.
According to one aspect of the invention, a sleeve is provided at the lower end of the collecting tray, an electric crushing roller is provided at the lower end of the sleeve, a filter is provided in the sleeve and at the upper end of the electric crushing roller, and a filter is provided in the sleeve at the upper end of the electric crushing roller. The crushing roller crushes the plants in the water body, and the filter filters out the fine solid impurities in the water body, and the solid impurities and plants in the water body enter the collection tray and block it, negatively impacting normal collection work. Avoid giving.
According to one aspect of the present invention, a collision prevention component is provided on the side wall of the sampling body, and the collision prevention component is a horizontally curved buffer plate connected to the side wall of the sampling body via a plurality of first spring connection columns. , a plurality of vertically curved buffer plates are connected to the horizontally curved buffer plates via a second spring connection post, and compared to a single layer structure buffer plate, the present device has horizontal curved buffer plates and vertically curved buffer plates. It forms a double buffer structure, which has better buffering capacity and can extend the service life of the shock absorber.
According to one aspect of the present invention, a buffer airbag is provided on the side wall of each of the vertically curved buffer plates, so that damage to the vertically curved buffer plates due to external force being directly applied to the vertically curved buffer plates can be avoided.
According to one aspect of the present invention, the first filtration membrane has a pore size of 0.45 μm, the second filtration membrane has a pore size of 0.2 μm, and the eukaryotic organisms in the water body are concentrated through the first filtration membrane. Then, the bacteria in the water body are concentrated through the second filtration membrane, and then the concentrated water suspension DNA is adopted, so that the collected sample is more complete and the detected data is more accurate.
The present invention further discloses an intelligent monitoring and sampling method for planktonic organisms in a seagrass bed ecosystem based on the above intelligent monitoring and sampling device for planktonic organisms in a seagrass bed ecosystem, which method comprises:
S1, control the external drive device through the controller to move the sampling body to the designated water environment area, start the drive motor, drive the rotating roller to rotate clockwise through the drive motor, and remove the winding rope from the rotating roller. is continuously lowered, and at this time, under the action of gravity of the main collection box, the collection sub-boxes on both sides of it slide downward in the mounting adjustment opening, and the collection tray moves synchronously into the water body, and the collection tray When reaches the preset collection depth,
controlling the external drive device to close through the controller;
S2, start the water pump, the water flows into the collection tray through each collection hole, enters the spray disk through the vertical collection pipe, and uniformly sprays on the first filtration membrane through the spray port; Concentrating eukaryotic organisms in the water environment through the first filtration membrane, water flows down through the first filtration membrane and falls into the collection sub-box from both sides of the inverted T-shaped fixed column, respectively, and then the second filtration membrane. Bacteria in the water environment are concentrated through the filtration membrane, water flows through the second filtration membrane into the water environment, the water flow rate sensor detects the amount of water that has entered the inverted T-shaped fixed column, and when the predetermined water amount is reached. , completing the collection of microbial DNA in the seagrass bed;
S3. When collecting samples, crush the plants in the water body through the electric crushing roller, filter the fine solid impurities in the water body through the filter, and use the horizontal curved buffer plate and vertical curved buffer plate to crush the plants in the water body.
forming a heavy buffer structure to block external forces impinging on the sampling body;
S4, when collecting samples, the wind flow blows perpendicularly to each blade, driving the sliding sleeve to move to the side closer to the mounting sliding plate, at this time, the sliding sleeve releases each rebound spring When compressed, the adjustment sliding rod pushes the corresponding counterbalanced sliding plate and slides along the left and right sides of the mounting sliding plate, respectively, so that the balanced sliding plate is located on the left and right sides of the sampling body, respectively, and and a balancing step.

先行技術と比較すると、本発明は以下の有益な効果を有する。
(1)本発明は海草藻場生態系における浮遊生物のインテリジェント監視およびサンプリ
ング装置およびサンプリング方法を提供し、取付調節口の内部に摺動可能に接続された収
集メインボックスを設置することにより、収集トレイの収集深度を容易に調節することが
できるだけでなく、異なる水域懸濁物を勾配で収集し、その後海草藻場生態系中の濃縮し
た水域懸濁物DNAを採用し、収集したサンプルがより完全で、検出したデータがより正
確である。
(2)本発明は、転倒防止部品をさらに設け、サンプルを収集するとき風速が大きいと、
平衡摺動板がそれぞれサンプリング本体の左右両側に摺動し、サンプリング本体の平衡を
取る目的を達成し、サンプリング本体の転倒を防止し、サンプリング本体の走行安定性を
向上させ、プロセス全体で外部の駆動部材を必要とせず、環境保護・省エネという利点を
有し、本装置を使用しないとき、2つの平衡摺動板が互いに近接してサンプリング本体の
下端に収容されるため、省スペース化が実現される。
(3)既存の単層緩衝構造と比較すると、本発明の衝突防止部品は、水平湾曲緩衝板と垂
直湾曲緩衝板で2重緩衝構造を形成し、より良好な緩衝能力を有し、同時に垂直湾曲緩衝
板の側壁に緩衝エアバッグがさらに設けられ、外力が垂直湾曲緩衝板に直接に当たって垂
直湾曲緩衝板が損傷することを避け、その使用寿命を大幅に延長することができる。
Compared with the prior art, the present invention has the following beneficial effects.
(1) The present invention provides an intelligent monitoring and sampling device and sampling method for planktonic organisms in a seagrass bed ecosystem, and the collection main box is slidably connected inside the installation control port. Not only can the collection depth of the tray be easily adjusted, but also different aquatic suspensions can be collected in a gradient, and then the concentrated aquatic suspension DNA in the seagrass bed ecosystem can be adopted, and the collected samples are more Complete and detected data is more accurate.
(2) The present invention further includes an anti-falling part, and when the wind speed is high when collecting the sample,
The balancing sliding plates slide on the left and right sides of the sampling body, respectively, to achieve the purpose of balancing the sampling body, prevent the sampling body from overturning, improve the running stability of the sampling body, and prevent external interference during the whole process. It does not require a driving member and has the advantages of environmental protection and energy saving, and when the device is not in use, the two balanced sliding plates are housed close to each other at the lower end of the sampling body, saving space. be done.
(3) Compared with the existing single-layer shock absorbing structure, the collision prevention component of the present invention forms a double shock absorbing structure with a horizontal curved shock absorber plate and a vertical curved shock absorber plate, which has better shock absorbing capacity, and at the same time A buffer air bag is further installed on the side wall of the curved buffer plate, which can avoid external forces directly hitting the vertical curved buffer plate and damage the vertical curved buffer plate, and greatly extend its service life.

本発明の三次元構造を示す模式図である。FIG. 1 is a schematic diagram showing a three-dimensional structure of the present invention. 本発明の断面図である。FIG. 2 is a cross-sectional view of the present invention. 本発明の転倒防止部品のサンプリング本体への取付構造を示す模式図である。FIG. 3 is a schematic diagram showing the attachment structure of the fall prevention component of the present invention to the sampling body. 本発明の転倒防止部品の底面図である。FIG. 3 is a bottom view of the fall prevention component of the present invention. 本発明の第1濾過膜の構造を示す模式図である。It is a schematic diagram showing the structure of the 1st filtration membrane of the present invention.

[符号の説明]
1 サンプリング本体
10 取付調節口
11 転倒防止部品
110 取付摺動板
111 逆T字形の固定柱
112 平衡摺動板
1120 摺動板
1121 平衡板
113 摺動スリーブ
1130 ブレード
1131 反発バネ
1132 調節摺動ロッド
114 制限遮断ブロック
12 衝突防止部品
120 水平湾曲緩衝板
1200 第1バネ接続柱
121 垂直湾曲緩衝板
1210 第2バネ接続柱
1211 緩衝エアバッグ
2 インテリジェント収集部品
20 収集メインボックス
200 収集サブボックス
21 第1濾過膜
210 取付内輪
211 取付外輪
212 分離ロッド
213 サブ濾過膜
22 第2濾過膜
220 外枠
23 エ字形の収集ヘッド
230 垂直収集管
231 噴霧ディスク
232 収集トレイ
233 噴霧口
234 収集孔
235 水流量センサー
236 スリーブ
2360 電動破砕ローラ
2361 フィルター
24 水ポンプ
3 駆動部品
30 H字形の取付ラック
31 回転ローラ
310 巻きロープ
32 駆動モータ
4 インテリジェント制御端末
40 コントローラ
41 GPSモジュール
42 無線通信モジュール
43 故障警報モジュール
[Explanation of symbols]
1 Sampling body 10 Mounting adjustment port 11 Fall prevention part 110 Mounting sliding plate 111 Inverted T-shaped fixed column 112 Balance sliding plate 1120 Sliding plate 1121 Balance plate 113 Sliding sleeve 1130 Blade 1131 Repulsion spring 1132 Adjustment sliding rod 114 Limiting blocking block 12 Collision prevention component 120 Horizontal curved buffer plate 1200 First spring connection column 121 Vertical curved buffer plate 1210 Second spring connection column 1211 Buffer airbag 2 Intelligent collection component 20 Collection main box 200 Collection sub box 21 First filtration membrane 210 Inner mounting ring 211 Outer mounting ring 212 Separation rod 213 Sub-filtration membrane 22 Second filtration membrane 220 Outer frame 23 E-shaped collection head 230 Vertical collection pipe 231 Spray disk 232 Collection tray 233 Spray port 234 Collection hole 235 Water flow sensor 236 Sleeve 2360 Electric crushing roller 2361 Filter 24 Water pump 3 Drive parts 30 H-shaped mounting rack 31 Rotating roller 310 Winding rope 32 Drive motor 4 Intelligent control terminal 40 Controller 41 GPS module 42 Wireless communication module 43 Failure alarm module

本発明の内容をさらに理解するために、以下、実施例を通じて本発明を詳細に説明する。
実施例1
図1および図2に示すように、海草藻場生態系における浮遊生物のインテリジェント監視
およびサンプリング装置は、中心に外部と連通する取付調節口10が設けられたサンプリ
ング本体1、取付調節口10に設けられたインテリジェント収集部品2、サンプリング本
体1の上端に設けられてインテリジェント収集部品2に接続された駆動部品3およびイン
テリジェント制御端末4を備え、
図5に示すように、インテリジェント収集部品2は、下端の左右両側に収集サブボックス
200が設けられた収集メインボックス20、収集メインボックス20内に設けられた第
1濾過膜21、収集サブボックス200内に設けられた第2濾過膜22、エ字形の収集ヘ
ッド23およびエ字形の収集ヘッド23に接続された水ポンプ24を含み、エ字形の収集
ヘッド23は、上端が収集メインボックス20の下端を貫通してその内部に延伸する垂直
収集管230、収集メインボックス20内に設けられて垂直収集管230の上端と連通す
る噴霧ディスク231、垂直収集管230の下端に接続された収集トレイ232を含み、
噴霧ディスク231の下端に複数の噴霧口233が均一に設けられ、収集トレイ232の
下端に複数の収集孔234が均一に設けられ、垂直収集管230に水流量センサー235
が設けられ、
第1濾過膜21と第2濾過膜22はいずれも着脱可能な構造であり、第1濾過膜21は垂
直収集管230の外壁に嵌設された取付内輪210、取付内輪210と同軸に設けられた
取付外輪211、取付内輪210と取付外輪211間に設けられた複数の分離ロッド21
2および隣接する2つの分離ロッド212間に可動に設けられたサブ濾過膜213を含み
、第2濾過膜22はシリコン膜およびシリコン膜を固定するための外枠220を含み、
駆動部品3は、サンプリング本体1の上端に設けられたH字形の取付ラック30、H字形
の取付ラック30の水平セグメントに設けられた回転ローラ31、回転ローラ31を回転
させるための駆動モータ32を含み、回転ローラ31の外壁に収集メインボックス20を
接続するための巻きロープ310が巻かれ、
インテリジェント制御端末4は、水ポンプ24と水流量センサー235に電気的に接続さ
れたコントローラ40、サンプリング本体1に設けられたGPSモジュール41、サンプ
リング本体1に設けられて遠隔信号転送に用いられる無線通信モジュール42、および故
障警報モジュール43を含み、
収集トレイ232の下端にスリーブ236が設けられ、スリーブ236の下端に電動破砕
ローラ2360が設けられ、スリーブ236内であって電動破砕ローラ2360上端にフ
ィルター2361が設けられ、
第1濾過膜21の孔径は0.45μmであり、第2濾過膜22の孔径は0.2μmである
In order to further understand the content of the present invention, the present invention will be described in detail below through Examples.
Example 1
As shown in FIGS. 1 and 2, the intelligent monitoring and sampling device for floating organisms in the seagrass bed ecosystem includes a sampling body 1 equipped with a mounting control port 10 in the center that communicates with the outside; an intelligent collecting part 2, a driving part 3 installed at the upper end of the sampling body 1 and connected to the intelligent collecting part 2, and an intelligent control terminal 4;
As shown in FIG. 5, the intelligent collection component 2 includes a collection main box 20 with collection sub-boxes 200 provided on both left and right sides of the lower end, a first filtration membrane 21 provided inside the collection main box 20, and a collection sub-box 200. It includes a second filtration membrane 22 provided inside, an E-shaped collection head 23 and a water pump 24 connected to the E-shaped collection head 23, the upper end of which is the lower end of the collection main box 20. a vertical collection tube 230 extending through and into the main collection box 20 , a spray disk 231 provided within the collection main box 20 and communicating with the upper end of the vertical collection tube 230 , and a collection tray 232 connected to the lower end of the vertical collection tube 230 . including,
A plurality of spray ports 233 are uniformly provided at the lower end of the spray disk 231, a plurality of collection holes 234 are uniformly provided at the lower end of the collection tray 232, and a water flow rate sensor 235 is provided in the vertical collection pipe 230.
is established,
Both the first filtration membrane 21 and the second filtration membrane 22 have a removable structure, and the first filtration membrane 21 is provided coaxially with the mounting inner ring 210 and the mounting inner ring 210 fitted to the outer wall of the vertical collection pipe 230. a plurality of separation rods 21 provided between the mounting outer ring 211 and the mounting inner ring 210 and the mounting outer ring 211;
2 and a sub-filtration membrane 213 movably provided between two adjacent separation rods 212, the second filtration membrane 22 includes a silicon membrane and an outer frame 220 for fixing the silicon membrane,
The drive component 3 includes an H-shaped mounting rack 30 provided at the upper end of the sampling body 1, a rotating roller 31 provided on a horizontal segment of the H-shaped mounting rack 30, and a drive motor 32 for rotating the rotating roller 31. A winding rope 310 for connecting the collection main box 20 is wound around the outer wall of the rotating roller 31;
The intelligent control terminal 4 includes a controller 40 electrically connected to the water pump 24 and the water flow rate sensor 235, a GPS module 41 provided in the sampling main body 1, and a wireless communication module provided in the sampling main body 1 for use in remote signal transmission. module 42 and a fault alarm module 43;
A sleeve 236 is provided at the lower end of the collecting tray 232, an electric crushing roller 2360 is provided at the lower end of the sleeve 236, and a filter 2361 is provided within the sleeve 236 and at the upper end of the electric crushing roller 2360;
The pore diameter of the first filtration membrane 21 is 0.45 μm, and the pore diameter of the second filtration membrane 22 is 0.2 μm.

実施例2
本実施例は以下の点で実施例1と異なる。
図3および図4に示すように、サンプリング本体1の下端に転倒防止部品11が設けられ
、転倒防止部品11は、サンプリング本体1の下端に垂直に設けられた取付摺動板110
、垂直セグメントが取付摺動板110の中心に可動に接続された逆T字形の固定柱111
、逆T字形の固定柱111の左右両側に対称に分布されて上端が取付摺動板110に摺動
可能に接続された平衡摺動板112、逆T字形の固定柱111の垂直セグメントの外部に
嵌設されて外壁が周方向に沿って均一に設けられた4つのブレード1130を有する摺動
スリーブ113を含み、摺動スリーブ113と逆T字形の固定柱111の水平セグメント
間に4つの反発バネ1131が均一に設けられ、摺動スリーブ113と各平衡摺動板11
2間に調節摺動ロッド1132がヒンジで接続され、
取付摺動板110の左右両側にそれぞれ制限遮断ブロック114が設けられ、平衡摺動板
112は、取付摺動板110に摺動可能に接続された摺動板1120、摺動板1120の
下方に設けられ長さが摺動板1120の長さよりも大きい平衡板1121を含み、平衡板
1121は中空構造であり、中空構造の平衡板1121内に発泡材が充填されている。
Example 2
This example differs from Example 1 in the following points.
As shown in FIGS. 3 and 4, a tipping prevention component 11 is provided at the lower end of the sampling body 1, and the tipping prevention component 11 includes a mounting sliding plate 110 vertically provided at the bottom end of the sampling body 1.
, an inverted T-shaped fixed column 111 with a vertical segment movably connected to the center of the mounting sliding plate 110
, a balanced sliding plate 112 distributed symmetrically on both left and right sides of the inverted T-shaped fixed column 111 and whose upper end is slidably connected to the mounting sliding plate 110, the outside of the vertical segment of the inverted T-shaped fixed column 111; The outer wall includes a sliding sleeve 113 having four blades 1130 uniformly provided along the circumferential direction, and four rebounds between the sliding sleeve 113 and the horizontal segments of the inverted T-shaped fixed column 111. Springs 1131 are uniformly provided, and the sliding sleeve 113 and each counterbalanced sliding plate 11
An adjustment sliding rod 1132 is connected by a hinge between 2,
Limiting blocking blocks 114 are provided on both left and right sides of the mounting sliding plate 110, and the balancing sliding plate 112 includes a sliding plate 1120 slidably connected to the mounting sliding plate 110, and a sliding plate 1120 below the sliding plate 1120. The balance plate 1121 is provided and has a length larger than the length of the sliding plate 1120, and the balance plate 1121 has a hollow structure, and the hollow structure of the balance plate 1121 is filled with foam material.

実施例3
本実施例は以下の点で実施例2と異なる。
図1および図2に示すように、サンプリング本体1の側壁に衝突防止部品12が設けられ
、衝突防止部品12は、複数の第1バネ接続柱1200を介してサンプリング本体1の側
壁に接続された水平湾曲緩衝板120、第2バネ接続柱1210を介して水平湾曲緩衝板
120に接続された複数の垂直湾曲緩衝板121を含み、
各垂直湾曲緩衝板121の側壁にそれぞれ緩衝エアバッグ1211が設けられる。
本実施例で使用される水流量センサー235、電動破砕ローラ2360、ガスポンプ15
2、水ポンプ24、回転ローラ31、駆動モータ32、コントローラ40、GPSモジュ
ール41、無線通信モジュール42および故障警報モジュール43はいずれも先行技術製
品であり、当業者は必要に応じて選択し、本発明の技術的解決策を満たすことができれば
よく、ここでは特に限定されない。
Example 3
This example differs from Example 2 in the following points.
As shown in FIGS. 1 and 2, a collision prevention component 12 is provided on the side wall of the sampling body 1, and the collision prevention component 12 is connected to the side wall of the sampling body 1 through a plurality of first spring connection columns 1200. including a horizontal curved buffer plate 120 and a plurality of vertical curved buffer plates 121 connected to the horizontal curved buffer plate 120 via a second spring connection post 1210;
A buffer airbag 1211 is provided on the side wall of each vertically curved buffer plate 121, respectively.
Water flow rate sensor 235, electric crushing roller 2360, and gas pump 15 used in this example
2. The water pump 24, the rotating roller 31, the drive motor 32, the controller 40, the GPS module 41, the wireless communication module 42 and the fault alarm module 43 are all prior art products, and those skilled in the art can select them according to their needs and understand the present invention. There is no particular limitation here as long as the technical solution of the invention can be satisfied.

実施例4
本実施例では、実施例1~3の海草藻場生態系における浮遊生物のインテリジェント監視
およびサンプリング装置に基づく、海草藻場生態系における浮遊生物のインテリジェント
監視およびサンプリング方法を開示し、この方法は、
S1、コントローラ40を通じて外部駆動装置を制御してサンプリング本体1を指定の水
環境領域に移動させ、駆動モータ32を起動し、駆動モータ32を通じて回転ローラ31
を時計回りに回転させるように駆動し、回転ローラ31から巻きロープ310が連続的に
巻き下げられ、このとき、収集メインボックス20の重力作用下で、その両側の収集サブ
ボックス200が取付調節口10内で下方に摺動し、収集トレイ232が同期して水域に
移動し、収集トレイ232が予設した収集深度に達すると、コントローラ40を通じて外
部駆動装置を閉じるように制御するステップと、
S2、水ポンプ24を起動し、水が各収集孔234を通って収集トレイ232に流入し、
垂直収集管230を通って噴霧ディスク231内に入り、噴霧口233を通って第1濾過
膜21上に均一に噴霧し、第1濾過膜21を通じて水環境中の真核生物を濃縮し、水が第
1濾過膜21を通って流下し、逆T字形の固定柱111の両側からそれぞれ収集サブボッ
クス200内に落下し、その後、第2濾過膜22を通じて水環境中の細菌を濃縮し、水が
第2濾過膜22を通って水環境に流れ、水流量センサー235により逆T字形の固定柱1
11に入った水量を検出し、予設水量に達すると、海草藻場中の微生物DNAの収集を完
了するステップと、
S3、サンプルを収集するとき、電動破砕ローラ2360を通じて水域中の植物を破砕し
、フィルター2361を通じて水域中の微細な固体不純物を濾過し、水平湾曲緩衝板12
0と垂直湾曲緩衝板121で2重緩衝構造を形成して、サンプリング本体1に衝突する外
力を遮断するステップと、
S4、サンプルを収集するとき、風の流れが各ブレード1130に垂直に吹き付け、摺動
スリーブ113を取付摺動板110に近い側方に移動させるように駆動し、このとき、摺
動スリーブ113は各反発バネ1131を圧縮し、調節摺動ロッド1132は、対応の平
衡摺動板112を押してそれぞれ取付摺動板110の左右両側に沿って摺動させ、平衡摺
動板112がそれぞれサンプリング本体1の左右両側に位置し、サンプリング本体1の平
衡を取るステップと、を含む。
Example 4
This example discloses a method for intelligent monitoring and sampling of planktonic organisms in a seagrass bed ecosystem based on the intelligent monitoring and sampling devices for planktonic organisms in a seagrass bed ecosystem of Examples 1 to 3, and this method includes:
S1, control the external drive device through the controller 40 to move the sampling body 1 to a designated water environment area, start the drive motor 32, and move the rotating roller 31 through the drive motor 32;
is driven to rotate clockwise, and the winding rope 310 is continuously unwound from the rotating roller 31, and at this time, under the action of gravity of the main collection box 20, the collection sub-boxes 200 on both sides of the main box 20 close to the mounting adjustment openings. 10, the collection tray 232 synchronously moves into the body of water, and controlling the external drive through the controller 40 to close when the collection tray 232 reaches a predetermined collection depth;
S2, start the water pump 24 and let water flow into the collection tray 232 through each collection hole 234;
It passes through the vertical collection pipe 230 into the spray disk 231 and passes through the spray port 233 to uniformly spray onto the first filtration membrane 21, concentrating the eukaryotic organisms in the water environment through the first filtration membrane 21, and flows down through the first filtration membrane 21 and falls into the collection sub-box 200 from both sides of the inverted T-shaped fixed column 111, and then passes through the second filtration membrane 22 to concentrate bacteria in the water environment and remove water from the water. flows into the water environment through the second filtration membrane 22, and the inverted T-shaped fixed column 1 is detected by the water flow rate sensor 235.
detecting the amount of water entering 11 and completing the collection of microbial DNA in the seagrass bed when the predetermined amount of water is reached;
S3. When collecting samples, crush the plants in the water body through the electric crushing roller 2360, filter fine solid impurities in the water body through the filter 2361, and use the horizontal curved buffer plate 12
0 and the vertically curved buffer plate 121 to form a double buffer structure to block external forces impinging on the sampling body 1;
S4, when collecting a sample, the wind flow blows vertically on each blade 1130 and drives the sliding sleeve 113 to move to the side closer to the mounting sliding plate 110, at this time, the sliding sleeve 113 By compressing each repulsion spring 1131, the adjusting sliding rod 1132 pushes the corresponding balancing sliding plate 112 to slide along the left and right sides of the mounting sliding plate 110, respectively, so that the balancing sliding plate 112 respectively slides on the sampling body 1. balancing the sampling body 1;

Claims (7)

中心に外部と連通する取付調節口(10)が設けられたサンプリング本体(1)、前記取
付調節口(10)に設けられたインテリジェント収集部品(2)、前記サンプリング本体
(1)の上端に設けられて前記インテリジェント収集部品(2)に接続された駆動部品(
3)およびインテリジェント制御端末(4)を備え、
前記インテリジェント収集部品(2)は、下端の左右両側に収集サブボックス(200)
が設けられた収集メインボックス(20)、前記収集メインボックス(20)内に設けら
れた第1濾過膜(21)、前記収集サブボックス(200)内に設けられた第2濾過膜(
22)、エ字形の収集ヘッド(23)および前記エ字形の収集ヘッド(23)に接続され
た水ポンプ(24)を含み、前記エ字形の収集ヘッド(23)は、上端が収集メインボッ
クス(20)の下端を貫通してその内部に延伸する垂直収集管(230)、収集メインボ
ックス(20)内に設けられて垂直収集管(230)の上端と連通する噴霧ディスク(2
31)、垂直収集管(230)の下端に接続された収集トレイ(232)を含み、前記噴
霧ディスク(231)の下端に複数の噴霧口(233)が均一に設けられ、前記収集トレ
イ(232)の下端に複数の収集孔(234)が均一に設けられ、垂直収集管(230)
に水流量センサー(235)が設けられ、
前記第1濾過膜(21)と第2濾過膜(22)はいずれも着脱可能な構造であり、第1濾
過膜(21)は垂直収集管(230)の外壁に嵌設された取付内輪(210)、前記取付
内輪(210)と同軸に設けられた取付外輪(211)、取付内輪(210)と前記取付
外輪(211)間に設けられた複数の分離ロッド(212)および隣接する2つの前記分
離ロッド(212)間に可動に設けられたサブ濾過膜(213)を含み、第2濾過膜(2
2)はシリコン膜およびシリコン膜を固定するための外枠(220)を含み、
前記駆動部品(3)は、サンプリング本体(1)の上端に設けられたH字形の取付ラック
(30)、前記H字形の取付ラック(30)の水平セグメントに設けられた回転ローラ(
31)、前記回転ローラ(31)を回転させるための駆動モータ(32)を含み、回転ロ
ーラ(31)の外壁に収集メインボックス(20)を接続するための巻きロープ(310
)が巻かれ、
前記インテリジェント制御端末(4)は、水ポンプ(24)と水流量センサー(235)
に電気的に接続されたコントローラ(40)、サンプリング本体(1)に設けられたGP
Sモジュール(41)、サンプリング本体(1)に設けられて遠隔信号転送に用いられる
無線通信モジュール(42)、および故障警報モジュール(43)を含む、ことを特徴と
する海草藻場生態系における浮遊生物のインテリジェント監視およびサンプリング装置。
A sampling body (1) provided with a mounting adjustment port (10) communicating with the outside at the center, an intelligent collection component (2) provided in the mounting adjustment port (10), and a sampling main body (1) provided at the upper end of the sampling main body (1). a driving part (
3) and an intelligent control terminal (4),
The intelligent collection part (2) has collection sub-boxes (200) on both left and right sides of the bottom edge.
a collection main box (20) provided with a collection main box (20), a first filtration membrane (21) provided in the collection main box (20), and a second filtration membrane (21) provided in the collection sub-box (200).
22), comprising an E-shaped collection head (23) and a water pump (24) connected to the E-shaped collection head (23), the upper end of which is connected to the collection main box ( a vertical collection tube (230) extending through and into the lower end of 20), a spray disk (2) provided within the collection main box (20) and communicating with the upper end of the vertical collection tube (230);
31), including a collection tray (232) connected to the lower end of the vertical collection pipe (230), with a plurality of spray ports (233) uniformly provided at the lower end of the spray disk (231), and the collection tray (232) ) are uniformly provided with a plurality of collection holes (234) at the lower end of the vertical collection tube (230).
A water flow rate sensor (235) is provided at
Both the first filtration membrane (21) and the second filtration membrane (22) have a removable structure, and the first filtration membrane (21) has a mounting inner ring (230) fitted into the outer wall of the vertical collection pipe (230). 210), an outer mounting ring (211) provided coaxially with the inner mounting ring (210), a plurality of separation rods (212) provided between the inner mounting ring (210) and the outer mounting ring (211), and two adjacent A sub-filtration membrane (213) is movably provided between the separation rods (212), and a second filtration membrane (213) is provided between the separation rods (212).
2) includes a silicon film and an outer frame (220) for fixing the silicon film;
The driving parts (3) include an H-shaped mounting rack (30) provided at the upper end of the sampling body (1), and a rotating roller (30) provided in the horizontal segment of the H-shaped mounting rack (30).
31), including a drive motor (32) for rotating said rotating roller (31), and a winding rope (310) for connecting the collection main box (20) to the outer wall of the rotating roller (31);
) is wound,
The intelligent control terminal (4) includes a water pump (24) and a water flow sensor (235).
a controller (40) electrically connected to a GP provided in the sampling main body (1);
Floating in a seagrass bed ecosystem characterized by comprising an S module (41), a wireless communication module (42) provided in the sampling body (1) and used for remote signal transmission, and a failure alarm module (43). Biological intelligent monitoring and sampling equipment.
前記サンプリング本体(1)の下端に転倒防止部品(11)が設けられ、前記転倒防止部
品(11)は、サンプリング本体(1)の下端に垂直に設けられた取付摺動板(110)
、垂直セグメントが前記取付摺動板(110)の中心に可動に接続された逆T字形の固定
柱(111)、前記逆T字形の固定柱(111)の左右両側に対称に分布されて上端が取
付摺動板(110)に摺動可能に接続された平衡摺動板(112)、逆T字形の固定柱(
111)の垂直セグメントの外部に嵌設されて外壁が周方向に沿って均一に設けられた複
数のブレード(1130)を有する摺動スリーブ(113)を含み、前記摺動スリーブ(
113)と逆T字形の固定柱(111)の水平セグメント間に複数の反発バネ(1131
)が均一に設けられ、摺動スリーブ(113)と各平衡摺動板(112)間に調節摺動ロ
ッド(1132)がヒンジで接続される、ことを特徴とする請求項1に記載の装置。
A fall prevention component (11) is provided at the lower end of the sampling body (1), and the fall prevention component (11) includes a mounting sliding plate (110) provided vertically at the lower end of the sampling body (1).
, an inverted T-shaped fixed column (111) with vertical segments movably connected to the center of the mounting sliding plate (110), symmetrically distributed on both left and right sides of the inverted T-shaped fixed column (111), and an upper end; a counterbalanced sliding plate (112) slidably connected to the mounting sliding plate (110), an inverted T-shaped fixed column (
a sliding sleeve (113) having a plurality of blades (1130) fitted on the outside of the vertical segment of the sliding sleeve (111), the outer wall of which has a plurality of blades (1130) uniformly provided along the circumferential direction;
A plurality of repulsion springs (1131) are installed between the horizontal segments of the inverted T-shaped fixed column (111)
) are uniformly provided and an adjusting sliding rod (1132) is hingedly connected between the sliding sleeve (113) and each counterbalance sliding plate (112). .
前記取付摺動板(110)の左右両側にそれぞれ制限遮断ブロック(114)が設けられ
、前記平衡摺動板(112)は、取付摺動板(110)に摺動可能に接続された摺動板(
1120)、前記摺動板(1120)の下方に設けられ長さが摺動板(1120)の長さ
よりも大きい平衡板(1121)を含み、前記平衡板(1121)は中空構造であり、中
空構造の平衡板(1121)内に発泡材が充填されている、ことを特徴とする請求項2に
記載の装置。
Limiting and blocking blocks (114) are provided on both left and right sides of the mounting sliding plate (110), and the balancing sliding plate (112) is a sliding block slidably connected to the mounting sliding plate (110). Board (
1120), including a balance plate (1121) provided below the sliding plate (1120) and having a length greater than the length of the sliding plate (1120), the balance plate (1121) having a hollow structure, and having a hollow structure. 3. Device according to claim 2, characterized in that the balance plate (1121) of the structure is filled with foam material.
前記収集トレイ(232)の下端にスリーブ(236)が設けられ、前記スリーブ(23
6)の下端に電動破砕ローラ(2360)が設けられ、スリーブ(236)内であって前
記電動破砕ローラ(2360)の上端にフィルター(2361)が設けられる、ことを特
徴とする請求項1に記載の装置。
A sleeve (236) is provided at the lower end of the collection tray (232), and the sleeve (236)
6) An electric crushing roller (2360) is provided at the lower end of the electric crushing roller (2360), and a filter (2361) is provided within the sleeve (236) and at the upper end of the electric crushing roller (2360). The device described.
前記サンプリング本体(1)の側壁に衝突防止部品(12)が設けられ、前記衝突防止部
品(12)は、複数の第1バネ接続柱(1200)を介してサンプリング本体(1)の側
壁に接続された水平湾曲緩衝板(120)、第2バネ接続柱(1210)を介して前記水
平湾曲緩衝板(120)に接続された複数の垂直湾曲緩衝板(121)を含む、ことを特
徴とする請求項1に記載の装置。
A collision prevention component (12) is provided on the side wall of the sampling body (1), and the collision prevention component (12) is connected to the side wall of the sampling body (1) via a plurality of first spring connection columns (1200). the horizontal curved buffer plate (120), and a plurality of vertical curved buffer plates (121) connected to the horizontal curved buffer plate (120) via a second spring connection post (1210). A device according to claim 1.
各前記垂直湾曲緩衝板(121)の側壁にそれぞれ緩衝エアバッグ(1211)が設けら
れる、ことを特徴とする請求項5に記載の装置。
6. Device according to claim 5, characterized in that the side walls of each vertically curved buffer plate (121) are respectively provided with a buffer air bag (1211).
請求項1~6のいずれか1項に記載の装置に基づく海草藻場生態系における浮遊生物のイ
ンテリジェント監視およびサンプリング方法であって、
S1、コントローラ(40)を通じて外部駆動装置を制御してサンプリング本体(1)を
指定の水環境領域に移動させ、駆動モータ(32)を起動し、駆動モータ(32)を通じ
て回転ローラ(31)を時計回りに回転させるように駆動し、回転ローラ(31)から巻
きロープ(310)が連続的に巻き下げられ、このとき、収集メインボックス(20)の
重力作用下で、その両側の収集サブボックス(200)が取付調節口(10)内で下方に
摺動し、収集トレイ(232)が同期して水域に移動し、収集トレイ(232)が予設し
た収集深度に達すると、コントローラ(40)を通じて外部駆動装置を閉じるように制御
するステップと、
S2、水ポンプ(24)を起動し、水が各収集孔(234)を通って収集トレイ(232
)に流入し、垂直収集管(230)を通って噴霧ディスク(231)内に入り、噴霧口(
233)を通って第1濾過膜(21)上に均一に噴霧し、第1濾過膜(21)を通じて水
環境中の真核生物を濃縮し、水が第1濾過膜(21)を通って流下し、逆T字形の固定柱
(111)の両側からそれぞれ収集サブボックス(200)内に落下し、その後、第2濾
過膜(22)を通じて水環境中の細菌を濃縮し、水が第2濾過膜(22)を通って水環境
に流れ、水流量センサー(235)により逆T字形の固定柱(111)に入った水量を検
出し、予設水量に達すると、海草藻場中の微生物DNAの収集を完了するステップと、
S3、サンプルを収集するとき、電動破砕ローラ(2360)を通じて水域中の植物を破
砕し、フィルター(2361)を通じて水域中の微細な固体不純物を濾過し、水平湾曲緩
衝板(120)と垂直湾曲緩衝板(121)で2重緩衝構造を形成して、サンプリング本
体(1)に衝突する外力を遮断するステップと、
S4、サンプルを収集するとき、風の流れが各ブレード(1130)に垂直に吹き付け、
摺動スリーブ(113)を取付摺動板(110)に近い側方に移動させるように駆動し、
このとき、摺動スリーブ(113)は各反発バネ(1131)を圧縮し、調節摺動ロッド
(1132)は、対応の平衡摺動板(112)を押してそれぞれ取付摺動板(110)の
左右両側に沿って摺動させ、平衡摺動板(112)がそれぞれサンプリング本体(1)の
左右両側に位置し、サンプリング本体(1)の平衡を取るステップと、を含む、ことを特
徴とする海草藻場生態系における浮遊生物のインテリジェント監視およびサンプリング方
法。
A method for intelligent monitoring and sampling of planktonic organisms in a seagrass bed ecosystem based on the device according to any one of claims 1 to 6, comprising:
S1, controlling the external drive device through the controller (40) to move the sampling body (1) to a designated water environment area, starting the drive motor (32), and driving the rotating roller (31) through the drive motor (32); Driven to rotate clockwise, the winding rope (310) is continuously unwound from the rotating roller (31), and at this time, under the action of gravity of the main collection box (20), the collection sub-boxes on both sides thereof (200) slides downward within the mounting control port (10), the collection tray (232) synchronously moves into the water body, and when the collection tray (232) reaches the preset collection depth, the controller (40) ) controlling the external drive device to close through the
S2, start the water pump (24) and let the water flow through each collection hole (234) to the collection tray (232).
), passes through the vertical collection tube (230) into the spray disc (231) and enters the spray nozzle (
233) and onto the first filtration membrane (21) to concentrate eukaryotic organisms in the aqueous environment through the first filtration membrane (21). The water flows down and falls into the collection sub-box (200) from both sides of the inverted T-shaped fixed pillar (111), and then passes through the second filtration membrane (22) to concentrate bacteria in the water environment, and the water The amount of water that flows into the water environment through the filtration membrane (22) and enters the inverted T-shaped fixed column (111) is detected by the water flow rate sensor (235), and when the predetermined amount of water is reached, the microorganisms in the seagrass bed are detected. completing the collection of the DNA;
S3. When collecting samples, crush the plants in the water body through the electric crushing roller (2360), filter the fine solid impurities in the water body through the filter (2361), and use the horizontal curved buffer plate (120) and the vertical curved buffer forming a double buffer structure with the plate (121) to block external forces impinging on the sampling body (1);
S4, when collecting samples, the wind flow blows perpendicularly to each blade (1130);
driving the sliding sleeve (113) to the side closer to the mounting sliding plate (110);
At this time, the sliding sleeve (113) compresses each repulsion spring (1131), and the adjusting sliding rod (1132) pushes the corresponding balance sliding plate (112) to move the left and right mounting sliding plates (110), respectively. a step of sliding along both sides and balancing the sampling body (1), with balancing sliding plates (112) located on the left and right sides of the sampling body (1), respectively; Intelligent monitoring and sampling methods for planktonic organisms in seaweed bed ecosystems.
JP2023119532A 2023-05-26 2023-07-23 Intelligent monitoring and sampling equipment and sampling method of planktonic organisms in seagrass bed ecosystem Active JP7377400B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202310604431.8 2023-05-26
CN202310604431.8A CN117126717A (en) 2023-05-26 2023-05-26 Intelligent sampling equipment and sampling method for plankton monitoring in seaweed bed ecosystem

Publications (1)

Publication Number Publication Date
JP7377400B1 true JP7377400B1 (en) 2023-11-10

Family

ID=88650844

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023119532A Active JP7377400B1 (en) 2023-05-26 2023-07-23 Intelligent monitoring and sampling equipment and sampling method of planktonic organisms in seagrass bed ecosystem

Country Status (2)

Country Link
JP (1) JP7377400B1 (en)
CN (1) CN117126717A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117589960A (en) * 2024-01-18 2024-02-23 浙江甬信检测技术有限公司 River water quality parameter comprehensive monitor
CN117723349A (en) * 2023-11-24 2024-03-19 青岛海洋地质研究所 Water sample automatic acquisition device for amphibious air-sea aircraft
JP7487436B1 (en) 2023-12-19 2024-05-21 広東智環研生態科技発展有限公司 Integrated sampling and separation device and method for detecting microplastics in water
CN117723349B (en) * 2023-11-24 2024-06-04 青岛海洋地质研究所 Water sample automatic acquisition device for amphibious air-sea aircraft

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117668472B (en) * 2024-02-02 2024-04-05 暨南大学 Island reef environment multi-parameter monitoring method and system

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002286597A (en) 2001-03-22 2002-10-03 Nichiyu Giken Kogyo Co Ltd Trigger for detection reach to bottom under submerged condition
JP2003189756A (en) 2001-12-26 2003-07-08 Mitsubishi Heavy Ind Ltd Method for extracting factor affecting marine ecosystem and method for controlling marine ecosystem
JP2004317504A (en) 2003-03-31 2004-11-11 K Mikimoto & Co Ltd Method for detecting harmful environment of water quality, and system for monitoring water quality environment
JP2007263893A (en) 2006-03-29 2007-10-11 Chugoku Electric Power Co Inc:The Ship for investigating plankton distribution
CN109162267A (en) 2018-10-31 2019-01-08 浙江海洋大学 A kind of collecting refuse from open water device
CN213275012U (en) 2020-10-19 2021-05-25 天津绿格瑞科技发展有限公司 Marine pollution sampling equipment
CN114239422A (en) 2022-02-23 2022-03-25 中国海洋大学 Method for improving marine chlorophyll a concentration prediction accuracy based on machine learning
JP2022101225A (en) 2020-12-24 2022-07-06 大成建設株式会社 Environmental dna sampling device and environmental dna sampling method
JP2022117928A (en) 2021-02-01 2022-08-12 生態環境部南京環境科学研究所 Intelligent collection and analysis device for integrated collection and analysis of underground water eluent

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002286597A (en) 2001-03-22 2002-10-03 Nichiyu Giken Kogyo Co Ltd Trigger for detection reach to bottom under submerged condition
JP2003189756A (en) 2001-12-26 2003-07-08 Mitsubishi Heavy Ind Ltd Method for extracting factor affecting marine ecosystem and method for controlling marine ecosystem
JP2004317504A (en) 2003-03-31 2004-11-11 K Mikimoto & Co Ltd Method for detecting harmful environment of water quality, and system for monitoring water quality environment
JP2007263893A (en) 2006-03-29 2007-10-11 Chugoku Electric Power Co Inc:The Ship for investigating plankton distribution
CN109162267A (en) 2018-10-31 2019-01-08 浙江海洋大学 A kind of collecting refuse from open water device
CN213275012U (en) 2020-10-19 2021-05-25 天津绿格瑞科技发展有限公司 Marine pollution sampling equipment
JP2022101225A (en) 2020-12-24 2022-07-06 大成建設株式会社 Environmental dna sampling device and environmental dna sampling method
JP2022117928A (en) 2021-02-01 2022-08-12 生態環境部南京環境科学研究所 Intelligent collection and analysis device for integrated collection and analysis of underground water eluent
CN114239422A (en) 2022-02-23 2022-03-25 中国海洋大学 Method for improving marine chlorophyll a concentration prediction accuracy based on machine learning

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117723349A (en) * 2023-11-24 2024-03-19 青岛海洋地质研究所 Water sample automatic acquisition device for amphibious air-sea aircraft
CN117723349B (en) * 2023-11-24 2024-06-04 青岛海洋地质研究所 Water sample automatic acquisition device for amphibious air-sea aircraft
JP7487436B1 (en) 2023-12-19 2024-05-21 広東智環研生態科技発展有限公司 Integrated sampling and separation device and method for detecting microplastics in water
CN117589960A (en) * 2024-01-18 2024-02-23 浙江甬信检测技术有限公司 River water quality parameter comprehensive monitor
CN117589960B (en) * 2024-01-18 2024-04-16 浙江甬信检测技术有限公司 River water quality parameter comprehensive monitor

Also Published As

Publication number Publication date
CN117126717A (en) 2023-11-28

Similar Documents

Publication Publication Date Title
JP7377400B1 (en) Intelligent monitoring and sampling equipment and sampling method of planktonic organisms in seagrass bed ecosystem
CN107727433A (en) A kind of soil sampling apparatus be easy to screen and remove stone in samples-soil
CN111537295B (en) Outdoor gas sampling and recycling device based on suspension mounting type for environmental protection detection
CN102901652A (en) Device for gathering algae moving vertically in reservoir
CN209961443U (en) All-round honeycomb formula dust and sand collection device
CN212441605U (en) Equipment for removing corncobs and light floating impurities by positive and negative pressure
CN208959507U (en) A kind of activated carbon adsorber with dust collect plant
CN201704436U (en) Lifting regulation platform of spinneret
CN111589570A (en) Equipment for removing corncobs and light floating impurities by positive and negative pressure
CN107807011A (en) A kind of water quality detection sampler for being easy to separation of solid and liquid
CN107022479A (en) A kind of bacterial reproduction case for carrying out sample comparison
CN113443721B (en) Fixed microalgae purifier for natural water and purification process thereof
CN206045610U (en) A kind of environment-friendly type public place air detection cleaner
CN113237704A (en) Sewage sampling detection device
CN202343029U (en) Energy-saving dedusting filter cartridge
CN114132439A (en) Accurate control system of water conservancy construction flow
CN212263814U (en) Boiling floating bed with good buffering performance
CN111732144B (en) Protein separator convenient to adjust
CN208270277U (en) A kind of air granule sampler
CN211576646U (en) Water pollution monitoring unmanned aerial vehicle water quality sampler
CN207662591U (en) Air-flow weakens formula gradient sand-taped instrument
CN213032952U (en) Screening device for spirulina
CN205280462U (en) On --spot filter equipment of water sample particle
CN215727092U (en) Water quality testing uses high-efficient water intake device
CN216080250U (en) Plasma laminar flow disinfection and purification system

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230724

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20230724

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230919

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230929

R150 Certificate of patent or registration of utility model

Ref document number: 7377400

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150