JP7371851B2 - Composition containing oleanane-type triterpene that prevents or improves physical fatigue and feeling of fatigue - Google Patents

Description

The present invention relates to an anti-fatigue composition containing, for example, an oleanane-type triterpene.

Along with pain and fever, fatigue is an important biological warning signal that expresses a state of overload caused by physical or mental causes, so much so that it is considered one of the three major biological alarms. However, the mechanisms that cause fatigue and the mechanisms that lead to the subjective sensation of fatigue (feeling of fatigue) have not been completely elucidated so far (Non-Patent Document 1; see "3. Biomarkers of Fatigue" on page 188). .

Fatigue in healthy people is defined by Kitani et al. of the research team of the Ministry of Health and Welfare's Fatigue Survey as ``a state of weakened mental and physical function accompanied by unique pathological discomfort and a desire for rest caused by excessive physical and mental activity.'' (Non-Patent Document 2; see "Introduction" on p. 200). Therefore, the "anti-fatigue" effect can be said to be an effect that alleviates the discomfort (feeling of fatigue) that occurs when some kind of overload is applied to the mind or body, or suppresses a decline in performance.

According to the anti-fatigue clinical evaluation guidelines established by the Japan Fatigue Society, fatigue refers to a decline in activity ability and efficiency caused by overload on the mind and body, and fatigue is the feeling of being aware of the presence of fatigue. Ru. Furthermore, as a method for evaluating anti-fatigue effects in human clinical trials, the authors cite measurement of subjective fatigue level and blood oxidative stress markers (Non-patent Document 3; p. 4, "4.1 Problems caused by daily life"). "Definition of Fatigue", p. 10 "Appendix"). A test comparing these multiple evaluation items is conducted, and if a statistically significant difference is observed, it is determined that the drug has a fatigue recovery effect. In particular, VAS (Visual Analogue Scale), which is a method for measuring the level of subjective fatigue, is considered to be useful as a method for evaluating fatigue in the anti-fatigue clinical evaluation of foods for specified health uses, and can be said to be a highly reliable method (not Patent Document 4).

According to a worker health status survey conducted by the Ministry of Health, Labor and Welfare in fiscal 2002, it was confirmed that 72% of workers said they felt tired from their daily work (Non-Patent Document 5; ``1. Physical fatigue''). and mental stress (see ``(1) Physical fatigue from regular work''). Nowadays, as social structures become more complex and speedy, and the population ages, fatigue and fatigue are expected to become more widespread in society, and countermeasures are needed to prevent overwork and revitalize the economy. ing.

As methods for recovering from fatigue, rest, sleep, bathing, nutrition, light exercise, aromatherapy, etc. have been used empirically. In particular, a widely used method for preventing physical fatigue caused by exercise is to ingest sufficient carbohydrates and amino acids before exercise to accumulate energy. Furthermore, in recent years, various food components that can be easily ingested have been proposed as anti-fatigue substances and anti-fatigue supplements. For example, it has been proposed that ingesting sesamin, imidazole dipeptide, sphingomyelin, catechins, resveratrol (Patent Documents 1 to 4 and Non-Patent Document 6), etc. is effective for preventing and recovering from fatigue.

On the other hand, oleanane-type triterpenes such as maslinic acid have the effect of promoting the effect of enhancing skeletal muscle function due to exercise (Patent Document 5), the effect of suppressing colorectal cancer (Non-Patent Document 7), and the skin whitening effect (Patent Document 6) , osteoclast differentiation inhibition and bone resorption activity effect (Patent Document 7), obesity prevention effect (Patent Document 8), blood sugar It has been reported that oleanolic acid suppresses the increase in oleanolic acid (Patent Document 9), has anti-caries effects (Patent Document 10), and has anticancer effects (Patent Document 11). However, none of these documents suggests or discloses any anti-fatigue effect or fatigue-reducing effect of oleanane-type triterpenes.

Japanese Patent Application Publication No. 2018-141014 Japanese Patent Application Publication No. 2002-173442 Japanese Patent Application Publication No. 2016-121132 Japanese Patent Application Publication No. 2005-089384 Japanese Patent Application Publication No. 2017-109942 International Publication No. 2002/043736 Japanese Patent Application Publication No. 2014-141444 International Publication No. 2003/011267 Japanese Patent Application Publication No. 2006-347967 Japanese Patent Application Publication No. 1-290619 Japanese Patent Application Publication No. 8-119866

Tanaka et al., Biomarkers of stress and fatigue, Japanese Pharmacological Journal, 2011, 137, pp. 185-188 Tanaka et al., Anti-fatigue effects of CBEX-Dr-containing beverages in healthy subjects, Jpn Pharmacol Ther (Pharmacology and Treatment), 2008, vol. 36, no. 3, pp. 199-212 Anti-fatigue clinical evaluation guidelines 5th edition, Japanese Fatigue Society, created July 22, 2011, pp. 1-13 Anti-fatigue clinical evaluation guidelines 5th edition, Japanese Fatigue Society, created July 22, 2011, attached Overview of the 2002 Worker Health Survey: Conditions of physical fatigue and mental stress, Ministry of Health, Labor and Welfare, August 2003 (https://www.mhlw.go.jp/toukei/itiran) /roudou/saigai/anzen/kenkou02/r1.html) Wu RE et al., Molecules, 2013, 18, pp. 4689-4702 Juan ME et al., J Nutr., 2006, 136(10), pp. 2553-2557

In view of the above-mentioned circumstances, the present invention aims to provide a composition for preventing and improving physical fatigue and fatigue caused by physical activities such as exercise.

As a result of intensive research to solve the above problems, it was discovered that fatigue and fatigue can be easily alleviated by ingesting a composition containing oleanane-type triterpenes, and the present invention was completed.

That is, the present invention includes the following.
(1) An anti-fatigue composition containing an oleanane-type triterpene, a salt thereof, or a prodrug thereof as an active ingredient.
(2) The anti-fatigue composition according to (1), wherein the oleanane-type triterpene is selected from the group consisting of maslinic acid, oleanolic acid, and mixtures thereof.
(3) An anti-fatigue composition containing as an active ingredient an olive fruit extract containing maslinic acid and oleanolic acid as major triterpenes.
(4) The anti-fatigue composition according to any one of (1) to (3), for preventing and/or improving physical fatigue and/or feeling of fatigue.
(5) An anti-fatigue food or drink containing the composition according to any one of (1) to (4).
(6) An anti-fatigue pharmaceutical containing the composition according to any one of (1) to (4).

According to the present invention, fatigue and fatigue caused by intense exercise such as water polo or muscle training can be prevented and improved.

An HPLC analysis chart of a liquid containing oleanane-type triterpenes derived from olive fruits is shown.

The present invention will be explained in detail below.

The anti-fatigue composition according to the present invention (hereinafter referred to as "composition according to the present invention") contains an oleanane-type triterpene, a salt thereof, or a prodrug thereof as an active ingredient. According to the composition according to the present invention, physical fatigue and/or fatigue feeling can be prevented and/or improved. Therefore, the composition according to the present invention can also be used as a preventive, therapeutic, or improving agent for physical fatigue and/or fatigue.

In the present invention, a triterpene is a compound having a basic skeleton of 30 carbon atoms, and a typical example thereof is a pentacyclic triterpene. Here, pentacyclic triterpene is a type of triterpene, and is a pentacyclic compound consisting of 6 isoprene units, with a basic number of carbon atoms of 30, but during the biosynthesis process, it undergoes rearrangement, oxidation, It also includes those with different carbon numbers due to elimination or alkylation.

These can be obtained from natural plants or artificially. Moreover, commercially available products can also be suitably used. Pentacyclic triterpenes are generally classified according to their skeleton. Examples include oleanane-type triterpenes, ursane-type triterpenes, lupine-type triterpenes, hopane-type triterpenes, seratane-type triterpenes, Friederan-type triterpenes, taraxerane-type triterpenes, taraxastan-type triterpenes, multiflorane-type triterpenes, Germanican-type triterpenes, and the like.

The composition according to the present invention contains an oleanane triterpene as an active ingredient among pentacyclic triterpenes. Examples of oleanane-type triterpenes include maslinic acid, oleanolic acid, and a mixture of maslinic acid and oleanolic acid.

Maslinic acid is found in olive fruits and leaves, jujubes, almonds, banaba leaves, sage, apples, cranberries, quince, etc. Oleanolic acid is found in olive fruits and leaves, grapes, beets, jujubes, almonds, banaba leaves, sage, hawthorn, raspberries, quince, rosemary leaves, guava, perilla leaves, blueberries, prunes, loquats, pomegranates, lemon balm, basil, rose. Contained in hips, oysters, and Japanese cabbage.

These oleanane-type triterpenes can be obtained by extraction and purification from the above-mentioned plants by known methods, or can also be obtained by chemical synthesis. The oleanane-type triterpene may be a single component or a mixture (combination) of two or more types of components.

Oleanane-type triterpenes may be used in the form of extracts of the above-mentioned plants. In particular, it is preferable to use the form of olive fruit extract because it has been eaten for a long time, contains abundant oleanane-type triterpenes, and can be used for secondary use after oil extraction. The olive fruit extract contains maslinic acid and oleanolic acid as major triterpenes.

As a method for producing oleanane-type triterpenes, for example, dried, semi-dried or undried olive fruits and olive oil lees produced in the oil extraction process are used as starting materials, and lower alcohols (ethanol, ethanol, etc.) from which triterpenes such as maslinic acid can be extracted are used. Examples include a method of extracting triterpenes with methanol, n-propanol, isopropanol, n-butanol, etc.) or a hydrous alcohol thereof. If necessary, perform saponification treatment and neutralization, and use octadecyl silica (ODS), silica gel, synthetic adsorbent, etc. as an adsorbent to fractionate and purify to obtain maslinic acid and/or oleanolic acid as the main components. A mixture of triterpenes containing as Triterpenes extracted from such olive fruits generally contain maslinic acid and oleanolic acid in a ratio of 4:1, but this ratio may vary depending on the growing conditions of the olive fruit, the growing region, or the oil extraction conditions. may be 1:1 to 7:1.

When using other plants as the starting material, oleanane-type triterpenes can be obtained by a method suitable for the plant used as the starting material. Moreover, the types of components and their composition ratios of the obtained oleanane-type triterpene depend on the plant used as a raw material.

The salt of the oleanane type triterpene is preferably a pharmaceutically acceptable salt, such as an inorganic acid such as hydrochloric acid, sulfuric acid, phosphoric acid, hydrobromic acid, hydroiodic acid, nitric acid, pyrosulfuric acid, metaphosphoric acid, etc. Citric acid, benzoic acid, acetic acid, propionic acid, fumaric acid, maleic acid, tartaric acid, succinic acid, sulfonic acid (e.g. methanesulfonic acid, p-toluenesulfonic acid, naphthalenesulfonic acid), amino acids (e.g. glutamic acid), etc. Examples include salts with organic acids, alkali metal salts such as sodium salts and potassium salts, lysine salts, and arginine salts.

Prodrugs of oleanane-type triterpenes or their salts are defined as all compounds that, when administered to biological systems, produce oleanane-type triterpenes or their salts as a result of spontaneous chemical reactions or by catalyzed enzymatic or metabolic reactions. show. Examples of groups constituting prodrugs used in the hydroxyl group include C _2-7 -acyl group, C _1-6 -alkoxy (C _2-7 acyl) group, and C _1-6 -alkoxycarbonyl (C _{2- 7} -acyl) group, C _1-6 -alkoxycarbonyl group, C _1-6 -alkoxy(C _2-7 -alkoxycarbonyl) group, (C _2-7 -acyloxy)methyl group, 1-(C _2-7 -acyloxy)ethyl group, (C _2-7 -alkoxycarbonyl)oxymethyl group, 1-[(C _2-7 -alkoxycarbonyl)oxy]ethyl group, etc., and C _2-7 -acyl group, C _{1 A -6} -alkoxycarbonyl group is preferred. Groups constituting prodrugs used in the carboxyl group include, for example, C _1-6 -alkyl group, C _1-6 -alkoxy-C _1-6 -alkyl group, (C _2-7 -acyloxy)methyl group. , 1-(C _2-7 -acyloxy)ethyl group, (C _2-7 -alkoxycarbonyl)oxymethyl group, 1-[(C _2-7 -alkoxycarbonyl)oxy]ethyl group, etc., and C _{1 A -6} -alkyl group and a _C1-6 -alkoxy- _C1-6 -alkyl group are preferred.

The amount of intake (dose) of the composition according to the present invention varies depending on the age, gender, severity of symptoms, and mode of intake (administration) of the subject who is ingesting (administering) the subject who has physical fatigue and/or feeling of fatigue. In terms of component (oleanane-type triterpene or its salt or prodrug thereof), for example, for adults, it is preferably 0.1 mg maslinic acid equivalent/kg body weight or more, more preferably 1.0 mg maslinic acid equivalent/kg body weight or more per day. . Although there is no particular upper limit for the intake (dose), it is undesirable from the viewpoint of product cost if it exceeds 60 mg maslinic acid equivalent/kg body weight.

The maslinic acid equivalent is the amount of a mixture of maslinic acid and a compound other than maslinic acid expressed by the mass of maslinic acid contained in the mixture. Specifically, 1.0 mg maslinic acid equivalent of olive fruit-derived triterpene refers to a mixture containing 1 mg maslinic acid with oleanolic acid or other peak compounds other than maslinic acid shown in the chart of Figure 1. means quantity.

The composition according to the present invention is administered in 1 to 3 doses per day, preferably for 1 to 2 weeks, more preferably for 1 to 20 weeks, depending on the period of exercise accompanied by fatigue or a feeling of fatigue, or daily life. Ingest (administer) separately. In addition, during the period of exercise or daily life accompanied by fatigue or a feeling of fatigue, regardless of the frequency of such exercise or daily life, i.e., on the day of exercise or the day of daily life accompanied by fatigue or a feeling of fatigue, It is also preferable to take (administer) the drug every day, even on days when you do not exercise or when you do not feel fatigued or fatigued in your daily life. The timing of intake (administration) does not matter, as long as it is a day of exercise or a day of daily life accompanied by fatigue or a feeling of fatigue, before or after the exercise or daily life of the day.

The composition according to the present invention can be formulated into any form together with auxiliary agents to form a pharmaceutical product that can be administered orally or parenterally. For example, oral dosage forms can be solid dosage forms such as tablets, rapidly disintegrating tablets, capsules, granules, granules, etc., and liquid dosage forms such as syrups and suspensions. Parenteral dosage forms include injections, eye drops, nasal drops, patches, suppositories, and skin preparations. Note that pharmaceuticals also include quasi-drugs.

When formulated into a solid dosage form, it may contain appropriate amounts of various additives used in the manufacture of general pharmaceutical preparations. Examples of such additives include excipients, binders, acidulants, foaming agents, artificial sweeteners, fragrances, lubricants, colorants, stabilizers, pH adjusters, surfactants, and the like.

When formulated into a liquid dosage form, the composition according to the present invention may be prepared in the presence of pH adjusting agents, buffering agents, solubilizing agents, suspending agents, etc., isotonic agents, stabilizers, preservatives, etc. as necessary. It can be formulated according to the method.

The form of external skin preparations is not particularly limited, and includes, for example, cosmetics such as emulsions, creams, lotions, packs, dispersions, cleaning agents, makeup cosmetics, scalp and hair products, ointments, creams, and external preparations. It can be a medicine such as a liquid preparation. In addition to the above ingredients, there are other ingredients commonly used in external skin preparations such as cosmetics and pharmaceuticals, such as whitening agents, moisturizers, various skin nutrition ingredients, ultraviolet absorbers, antioxidants, oily ingredients, surfactants, and thickeners. , alcohols, coloring agents, water, preservatives, fragrances, etc. may be appropriately blended as required.

The composition according to the present invention can be prepared as a functional food, a health food, a food for specified health uses, a food with health claims such as a food with nutritional function, a food for special purposes (e.g., food for patients), a health supplement, a supplement, etc. It's okay. For example, the composition according to the present invention can be used in a food with functional claims such as "prevents and/or improves physical fatigue and/or feeling of fatigue." Supplements include, for example, tablets, rounds, capsules (including hard capsules, soft capsules, and microcapsules), powders, granules, fine granules, troches, and jelly along with various additives used in the manufacture of general supplements. It can be in the form of a liquid (including syrup, emulsion, suspension), etc.

The composition according to the present invention can be incorporated into food and drink products. Foods and drinks that can be mixed are not particularly limited, but include confectionery such as candy, gummies, and chewing gum; confectionery such as cookies, crackers, biscuits, chocolate, pudding, jelly, snacks, rice crackers, manju, and yokan. Frozen desserts such as ice cream, popsicles, sherbet, and gelato; Bakery foods such as donuts, cakes, white bread, French bread, and croissants; Noodles such as udon, soba, Chinese noodles, and kishimen; Rice products such as white rice, sekihan, and pilaf; Examples include sauces such as curry, stew, and dressing; paste products such as ham, sausage, kamaboko, chikuwa, and fish sausage; various side dishes such as tempura, croquettes, and hamburgers; and beverages such as juice and tea.

The effects of the composition according to the present invention are, for example, measured by VAS, which is a method of measuring subjective fatigue (e.g., "feeling of whole body fatigue" as an analysis item; Non-Patent Document 4), and blood oxidative stress markers (e.g., TBARS (Thio barbituric acid reactive substances (2-thiobarbituric acid reactive substances); Novel anti-fatigue ingredient: imidazole dipeptide, Journal of the Japanese Society of Complementary and Alternative Medicine, Volume 6, Issue 3, October 2009: 123-129 and Shuji Oishi , Exercise and Oxidative Stress - The Importance of the Balance between Active Oxygen and Antioxidant Defense -, IRYO, Vol. 69, No. 7 (317-324) 2015). For example, the composition according to the present invention is ingested (or administered) to a subject who exercises with fatigue or a feeling of fatigue, or a subject who lives a daily life with fatigue or a feeling of fatigue. If the measured value of VAS or blood oxidative stress marker is significantly lower than that of a control group that does not take (or administer) the composition of the present invention, the composition of the present invention has an anti-fatigue effect. It can be determined that the

On the other hand, according to the above description of the composition according to the present invention, the present invention provides a method for reducing physical fatigue and/or physical fatigue, including administering or ingesting the composition according to the present invention to a patient or subject (human). The present invention also relates to methods for preventing, treating, and/or improving fatigue.

Hereinafter, the present invention will be explained in more detail using Examples, but the technical scope of the present invention is not limited to these Examples.

[Evaluation of the effects of olive fruit-derived triterpenes on fatigue and fatigue]
1. Production example 1 [Production method of olive fruit-derived triterpene]
Olive fruit pressed oil cake was obtained from which olive oil was removed by a known oil pressing method such as squeezing.

Add 250 g of 90% aqueous ethanol (v/v) to 100 g of this olive fruit oil residue, heat it at 70°C for 2 hours with stirring, and then distill off the liquid part obtained by solid-liquid separation under reduced pressure using an evaporator or the like. A concentrated solution was obtained.

This concentrated solution was dispersed in water and subjected to column chromatography using synthetic adsorption resin Amberlite The containing fraction was obtained and distilled under reduced pressure to obtain an olive fruit-derived oleanane-type triterpene in the form of a liquid oil.

FIG. 1 is an HPLC analysis chart of a liquid containing oleanane-type triterpenes derived from olive fruits. The A peak is maslinic acid, and the B peak is oleanolic acid. Peak identification was performed by comparison of retention times with standard products and LC/MS analysis. The quantitative value was calculated based on the peak area of the standard product. This triterpene-containing liquid contained maslinic acid and oleanolic acid in a ratio of 10:4.

Dextrin was added and mixed so that the maslinic acid content was 10% by mass, dispersed in water, and then spray-dried to obtain a powdered olive fruit-derived triterpene agent.

2. Production example 2 [Preparation of test food]
Raw materials were mixed according to the formulation shown in Table 1 to prepare stick jelly. The content per stick jelly is 10 g, and the contents of maslinic acid and oleanolic acid are 30 mg and 12 mg, respectively.

3. Test Example 1 [Evaluation of the influence of olive fruit-derived triterpenes on fatigue and fatigue feeling]
(1) Selection of subjects Twelve healthy male university students who belong to the water polo club of the University of Tsukuba were selected as subjects.
(2) Test details In order to evaluate the effects of olive fruit-derived triterpenes on exercise and the fatigue and fatigue that occur in daily life, subjects underwent an exercise intervention including water polo practice and strength training shown in Table 2 from Monday to Sunday. I went for a week. At that time, the test food containing the olive fruit-derived triterpene agent shown in Production Example 2 (Example) or the test food not containing it (Comparative Example) were ingested twice daily after exercise.

The study design was a double-blind crossover study, and the subjects were randomly divided into two groups, A and B. For one week of the first course, group A received the stick jelly of the example, and group B received the comparative example. After ingesting the stick jelly and allowing one week during which they did not ingest the test food as a cleansing period, in the second course of one week, Group A was allowed to ingest the stick jelly of the comparative example, and Group B was allowed to ingest the stick jelly of the example.

The results of the first and second courses were combined to obtain the measured values for 12 people in each example and comparative example, and the averages were compared and analyzed.

(3) Method
(3-1) Measurement of subjective fatigue level As an item to evaluate the physical fatigue and feeling of fatigue caused by exercise, the subjective fatigue level when waking up the day after exercise was evaluated continuously for one week using VAS. For example, the evaluation of subjective fatigue level by VAS when waking up on Tuesday is a feeling of fatigue that appeared as a result of Monday's exercise and daily life, so it was recorded as Monday's value (Table 3). In addition, the VAS indicates where the current state is on the straight line, with the left end of the 10cm straight line being 0, which means ``I don't feel tired at all,'' and the right end being 10, which is ``I feel extremely tired.'' The students were asked to mark the value of the 10 cm line with a bar that intersects perpendicularly with the 10 cm straight line, which was then used as the measured value. Based on the measured values, statistical analysis was performed using a T-test for each day of the week.

(3-2) Measurement of blood oxidative stress markers It is possible to evaluate the degree of fatigue by measuring oxidative stress markers (New anti-fatigue ingredient: imidazole dipeptide, Journal of the Japanese Society of Complementary and Alternative Medicine, Volume 6, No. 3, October 2009: 123-129; see page 124 “4. Fatigue evaluation using oxidative stress markers”).

Blood was collected on the morning of each Monday during the test period, and the blood TBARS (Thio barbituric acid reactive substances) concentration before and after ingestion of the test food was measured using a TBARS measurement kit (OxiSelect TBARS Assay Kit). , CELL BIOLABOS, Inc.). TBARS performed statistical analysis using T-test for comparisons before and after the intervention of each group, and 2-way ANOVA for comparisons between groups.

TBARS is a general term for substances whose concentration increases in response to oxidative stress, and includes lipid hydroperoxides and aldehydes such as malondialdehyde. In addition, TBARS has been widely studied as an indicator of exercise-induced oxidative stress, and its levels have been shown to increase in skeletal muscle, cardiac muscle, liver, and plasma after acute exercise (Shuji Oishi, Exercise Research and oxidative stress - the importance of the balance between active oxygen and antioxidant defenses -, IRYO, Vol. 69, No. 7 (317-324) 2015; see p. 319 “1. Acute exercise”).

(4) Results
As shown in Table 3, the results of measuring the subjective fatigue level by VAS showed that the Example showed a lower value than the Comparative Example throughout the entire day. As a result of statistical analysis, VAS values were significantly lower, especially on Saturdays when the test food intake was continued for 6 days (P value <0.05).

In addition, Cohen's d was calculated to analyze the effect size (effect size) of ingesting triterpenes derived from olive fruits. Cohen's d is a calculated value used when comparing the difference between the means of two groups, such as in a t-test, and the calculated value represents how far apart the means are in terms of standard deviation. There is. For example, d = 1 means they are 1 standard deviation (SD) apart. Furthermore, a Cohen's d value of 0.8 or higher is considered a "large effect," a value of 0.5 or higher is a "medium effect," a value of 0.2 or higher is a "small effect," and a value of less than 0.2 is considered "almost no effect." (Mizumoto et al. , Introduction to effect size and power analysis: How to use statistical tests correctly; 2010 Subcommittee Report Collection "Methods for Better Foreign Language Education": 47-73, Issue Date: 2011-06-06).

As a result of comparing the effect sizes based on Cohen's d, there was a tendency for the effect sizes to increase as the week progressed (Table 3).

Furthermore, as a result of measuring blood TBARS concentration, as shown in Table 4, blood TBARS concentration increased significantly after the intervention compared to before intervention in the comparative example, whereas in the example example compared to before intervention. The blood TBARS concentration was significantly lower after the intervention (P value (before and after comparison) <0.05). Furthermore, when the amount of change in each case was compared between groups, a significant difference was found (P value (comparison between groups) <0.05).

The subjective fatigue level measured by VAS was lower in the example than in the comparative example, indicating that ingestion of olive fruit-derived triterpenes improves the feeling of physical fatigue that occurs during exercise. It became clear (Table 3). Furthermore, as the effect size increases as the week progresses, it is possible that ingesting triterpenes derived from olive fruits after exercise not only improves fatigue on the day of exercise, but also prevents fatigue the next day. It was suggested. In other words, triterpenes derived from olive fruits can also be expected to have anti-fatigue effects.

Taking a closer look at the results, we found that the VAS values were the lowest on Sundays (measurements were taken when waking up on Monday) because the day was off. Also, since I had water polo practice on Monday, my fatigue level was slightly higher than on Sunday. Since strength training was performed on Tuesdays and Thursdays, it was assumed that the feeling of fatigue increased after peaking on Tuesdays and Thursdays, and that the feeling of fatigue improved as time passed from the strength training.

Furthermore, blood TBARS concentrations were lower in Examples than in Comparative Examples (Table 4), suggesting that blood oxidative stress levels were reduced.

These results suggest that ingesting olive fruit-derived triterpenes reduces oxidative stress caused by exercise and prevents and improves physical fatigue.

Claims (4)

An anti-fatigue composition containing an oleanane-type triterpene or a salt thereof as an active ingredient,
the oleanane-type triterpene is a mixture of maslinic acid and oleanolic acid,
The anti-fatigue composition (excluding cases containing 3-hydroxy-3-methylbutyric acid ) for preventing and/or improving physical fatigue and/or fatigue caused by exercise. An anti-fatigue composition containing as an active ingredient an olive fruit extract containing maslinic acid and oleanolic acid as major triterpenes,
The anti-fatigue composition (excluding cases containing 3-hydroxy-3-methylbutyric acid ) for preventing and/or improving physical fatigue and/or fatigue caused by exercise. An anti-fatigue food or drink comprising the composition according to claim 1 or 2. An anti-fatigue pharmaceutical comprising the composition according to claim 1 or 2.

Images