JP7366004B2 - Plant growth agent containing cystenylglycine - Google Patents

Description

The present invention particularly provides a plant growth agent that can achieve early harvest of agricultural crops, increase yield, improve nutritional value, and alleviate environmental stress.

There are various growth promoters, plant growth regulators, physiological activity promoters, taste improvers, etc. that are sprayed on the leaves and soil of vegetables and fruit trees to increase the yield and improve the quality of agricultural products such as vegetables. .

Examples of sulfur-containing compounds that have been recognized to be effective in increasing the yield and improving the quality of agricultural crops include plant growth regulators containing oxidized glutathione (Patent Documents 1, 2, and 3). In addition, it is known that acetylcysteine is effective in preventing pests and diseases (Patent Document 4), and foliar sprays containing cysteine (Patent Document 5). It has been reported that there is a growth promoting effect and a root elongating effect (Patent Document 6).
On the other hand, there is a report that cystenylglycine, which is a sulfur-containing compound, is used as a Maillard base material for seasonings and the like (Patent Document 7), but there is no report that it is involved in promoting plant growth.

International Publication No. 2008/072602 International Publication No. 2013/002317 International Publication No. 2017/006869 Special table 2008-538566 publication Japanese Patent Application Publication No. 05-330968 International Publication No. 2016/148193 Japanese Patent Application Publication No. 2001-321117

An object of the present invention is to provide a plant growth agent with excellent plant growth promoting effects.

The present inventors have found that by using cystenylglycine, it is possible to promote plant growth and prevent pests by improving resistance, and have completed the present invention.

That is, the present invention is a plant growth agent containing a compound represented by the following general formula (1), and a plant growth agent containing cystenylglycine or a derivative, salt, ester, or amide compound thereof. Additionally, plants may generate oxidative stress and free radicals within cells due to environmental stress, but these can be reduced by the antioxidant effect of cystenylglycine.

Cystenylglycine, as described herein, is an antioxidant. The cystenylglycine of the present invention is very useful in controlling environmental stress, pests and pathogens. It can also be applied to plants and crops to improve yields. Furthermore, plants with increased nutritional value can be grown.

By spraying cystenylglycine to plants, it is possible to obtain excellent growth-promoting effects and root elongation-promoting effects in tomatoes, Japanese mustard spinach, and Japanese radish.

Change in weight of Komatsuna due to foliar spraying of glutathione-related substances Tomato growth status (left control, right CG 0.5mM)

The present invention will be explained in detail below.
The plant growth agent of the present invention only needs to contain cystenylglycine, and the specific composition of other components is not particularly limited. Can be blended.

The method for obtaining cystenylglycine of the present invention is not particularly limited. For example, it can be obtained using chemical, enzymatic, or microbial fermentation production methods, and generally commercially available products can also be used. A specific example of a method using fermentation production is a method in which glutathione is obtained from yeast cells that contain a high content of glutathione. In addition, after culturing yeast with high glutathione content, collecting and washing the bacteria, enzymes inside the bacteria were inactivated with hot water, and then enzymes such as glutaminase were added to decompose glutathione into cystenylglycine and glutamic acid. It is also possible to use a product that is subsequently purified using a resin or the like.

Examples of yeast include baker's yeast, brewer's yeast (Saccharomyces cerevisiae), torula yeast (Candida utilis), and the like. Among them, Torula yeast, which is generally said to have a high glutathione content, is desirable because it contains a lot of cystenylglycine.

The plant growth agent of the present invention can be expected to promote plant growth, root elongation, and improve nutritional value. Plants include root vegetables, fruit vegetables, and grains. Examples include tomatoes, komatsuna, radish, spinach, onions, grapes, apples, pears, melons, and strawberries.
Promoting root elongation also improves the absorption of nutrients and promotes the growth of vegetables and fruit trees, making it possible to increase yields or harvest earlier. Furthermore, it can be expected to improve nutritional value.

The dosage form of the present invention can be, for example, a solid dosage form or a liquid dosage form. For example, it can be made into a solid preparation during distribution and storage, and can be dissolved in water at an appropriate concentration to form a liquid preparation. Alternatively, it can be kept in a concentrated state during distribution and storage, and diluted with water to an appropriate concentration when used. The concentration of cystenylglycine during use can be preferably 0.01 to 1000 mM, more preferably 0.1 to 10 mM. When the cystenylglycine concentration is within this range, the yield and nutritional value of agricultural crops will improve. In the present invention, the cysteinylglycine concentration is measured as follows.
(Cystenylglycine measurement method)
The cystenylglycine content in the sample is determined by high performance liquid chromatography (HPLC). The cystenylglycine specimen (H-Cys-Gly-OH) manufactured by Bachem is used. The HPLC conditions were: separation column Unison US-C18 (5 μm, 150 mm x 4.6 mm, Imtakt), mobile phase: phosphate buffer (pH 3.2), flow rate: 1 mL/min, detection: ultraviolet detector (210 nm). )

The frequency of spraying the cystenylglycine of the present invention is not particularly limited, but it is preferably sprayed at least once every 1 to 100 days, and more preferably once every 7 to 10 days. Cystenylglycine may be used alone or in combination with fertilizer or horticultural soil. Fertilizers include chemical fertilizers containing nitrogen, phosphoric acid, and potassium, and organic fertilizers such as vegetable oil cake, fish meal, and seaweed powder. Application methods include contact with plants or soil, foliar spraying, spreading, and addition to a solution during hydroponic cultivation.

The present invention will be described in detail with reference to Examples, but the present invention is not limited to these Examples.

<Example 1>
Komatsuna seeds were sown in flowerpots (16 cm x 16 cm x 17 cm) using commercially available "Flower and Vegetable Soil" as soil, and harvested about 90 days later. Foliar spraying was performed once every 10 days after the appearance of true leaves using a sample dissolved in water to the concentration shown below. The spray was applied until the underside of the leaves were completely wet. No temperature control was performed. As a test group, 0.5mM of cystenylglycine CG (manufactured by Bachem) was prepared.

<Comparative example 1>
The same procedure as in Example 1 was conducted except that 0.1 mM of oxidized glutathione GSSG (manufactured by Wako) was used as the test group.

<Comparative example 2>
The same procedure as in Example 1 was conducted except that 0.5 mM of oxidized glutathione GSSG (manufactured by Wako) was used as the test group.

The results of Example 1, Comparative Example 1, and Comparative Example 2 are shown in FIG.

Compared to the control (water) and the test plots containing oxidized glutathione 0.1mM or 0.5mM, the growth amount clearly increased in the test plot containing 0.5mM cystenylglycine.
By spraying the cystenylglycine-containing plant growth agent of the present invention on the leaves of plants, an excellent growth promoting effect can be obtained, and early harvest and increased yield can be expected.

<Example 2> Growth promoting effect on tomatoes Tomato seeds (Momotaro) were sown in pots using commercially available "Flower and Vegetable Soil" as soil, and 15 days after sowing, "Flower and Vegetable Soil" was similarly used as soil. Three plants were planted in each planter (80 x 30 cm) using ``soil''. One week after planting, 0.5mM of cystenylglycine was applied to the leaves twice every 10 days. Spraying was carried out until the undersides of the leaves were completely wet. The growth status of the plants was confirmed two months after sowing. No particular temperature control was performed.

The results of Example 2 are shown in FIG.

In the test plot where 0.5mM of cystenylglycine was sprayed on the leaves, tomato seedlings grew significantly better than in the untreated plot, and the stems were longer and the leaves were larger.

From the results of this example, it was confirmed that the plant growth agent containing cystenylglycine as an active ingredient of the present invention promotes the growth of plants in general, including vegetables and fruits.

Claims (1)

A foliar spray containing 0.1-10mM of cystenylglycine.