JP7337640B2 - Centrifuge and white blood cell counter - Google Patents

Description

The present invention relates to centrifugation of leukocytes in raw milk.

Conventionally, centrifugation of raw milk is known (see, for example, Patent Documents 1, 2 and 3). When the raw milk is centrifuged, the leukocytes and fat in the raw milk are separated, and the leukocytes eventually settle to the bottom. The number of precipitated leukocytes is measured and used to detect mastitis.

JP 2011-033599 A Japanese Patent Publication No. 2012-521219 Japanese Patent Publication No. 6-500462

However, according to the centrifugation in the prior art as described above, sedimentation of leukocytes takes a long time.

Accordingly, an object of the present invention is to enable detection of components (for example, white blood cells) in centrifuged raw milk in a short period of time.

The centrifugal separator according to the present invention includes a container for containing raw milk, a rotating body arranged inside the container and rotating about the central axis of the container, and a component of the raw milk protruding from the side surface of the container. The side surface intersects the bottom surface of the container, and the bottom surface is configured to intersect the central axis.

According to the centrifuge configured as described above, the container contains the raw milk. A rotating body is disposed inside the container and rotates around the central axis of the container. An ingredient storage part protrudes from the side surface of the container and stores the ingredients of the raw milk. The side surface intersects the bottom surface of the container. The bottom surface intersects the central axis.

In addition, in the centrifugal separator according to the present invention, the component storage section may have a constant depth from the side surface.

In addition, in the centrifugal separator according to the present invention, the vertical interval between the component storage portions may be narrowed as the distance from the central axis increases.

In addition, in the centrifugal separator according to the present invention, the rotor may be a screw.

In addition, in the centrifugal separator according to the present invention, an adsorbent for adsorbing white blood cells in the raw milk may be arranged in the component storage section.

In addition, in the centrifugal separator according to the present invention, the adsorbent may be P-selectin.

In addition, in the centrifugal separator according to the present invention, a storage portion for storing the component may be arranged at a portion where the component storage portion is most distant from the central axis.

In addition, the centrifugal separator according to the present invention may include a pipe connected to the reservoir and a valve for opening and closing the pipe.

A white blood cell amount measuring apparatus according to the present invention comprises a centrifuge according to the present invention, a sensor for detecting white blood cells obtained by the centrifuge, and a display unit for displaying the white blood cell amount based on the detection result of the sensor. is configured to include

A white blood cell amount measuring apparatus according to the present invention comprises a centrifuge according to the present invention, a sensor connected to the piping for detecting white blood cells stored in the reservoir, and measuring the white blood cell amount based on the detection result of the sensor. and a display for displaying.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a diagram showing the configuration of a white blood cell amount measuring device 1 according to a first embodiment of the present invention, in which a screw (rotating body) 14 is arranged on the lower side inside a container 12 (FIG. 1(a)); 12 (FIG. 1(b)). The internal state of the container 12 and the component storage unit 13 before centrifugation in the first embodiment (Fig. 2(a)) and the internal state of the container 12 and the component storage unit 13 after centrifugation (Fig. 2(b)) It is a figure which shows. FIG. 3A is a plan view (FIG. 3A), a front view (FIG. 3B), and a side view (FIG. 3C) showing the configuration of a white blood cell amount measuring device 1 according to a second embodiment of the present invention; )). The state of the inside of the container 12, the component storage part 18, and the storage part 19 before centrifugation in the second embodiment (FIG. 4A) and the inside of the container 12, the component storage part 18, and the storage part 19 after centrifugation It is a figure which shows the state (FIG.4(b)).

Hereinafter, embodiments of the present invention will be described with reference to the drawings.

First Embodiment FIG. 1 is a diagram showing the configuration of a white blood cell amount measuring device 1 according to a first embodiment of the present invention, in which a screw (rotating body) 14 is arranged on the lower side inside a container 12. (FIG. 1(a)) and the state arranged on the upper side in the container 12 (FIG. 1(b)).

A white blood cell amount measuring device 1 according to the first embodiment includes a centrifuge 10 , a sensor 22 and a display section 24 .

The centrifugal separator 10 includes a pipe 11 , a container 12 , a component container 13 , a screw (rotating body) 14 , a shaft 15 , a motor 16 and a valve 17 .

The container 12 is a container that contains the raw milk 2 . Side 12S of container 12 intersects bottom 12B of container 12 . In the example of FIG. 1, container 12 is a cylindrical container, and side surface 12S is perpendicular to bottom surface 12B and top surface 12T of container 12. In the example of FIG. Bottom surface 12B intersects central axis A of container 12 . In the example of FIG. 1, the bottom surface 12B and the top surface 12T are orthogonal to the central axis A. In FIG.

A screw (rotating body) 14 is arranged inside the container 12 and rotates about the central axis A as a rotation axis. The screw 14 may be arranged on the lower side of the container 12 (see FIG. 1(a)) or may be arranged on the upper side of the container 12 (see FIG. 1(b)).

The shaft 15 is the rotating shaft of the screw 14 and is arranged on the central axis A. As shown in FIG. A motor 16 is connected to the screw 14 via a shaft 15 and rotates the screw 14 .

The component storage part 13 protrudes from the side surface 12S of the container 2 and stores components of the raw milk 2 (for example, white blood cells). The component containing portion 13 is in the shape of a groove having a constant depth t from the side surface 12S. An adsorbent (for example, P-selectin) that adsorbs leukocytes in the raw milk 2 is placed in the ingredient storage section 13 .

The pipe 11 is connected to the component storage section 13 . The valve 17 is a valve that opens and closes the pipe 11 .

Sensor 22 detects white blood cells (eg, neutrophils) obtained by centrifuge 10 . More specifically, the sensor 22 receives the leukocytes contained in the component container 13 via the pipe 11 and detects the leukocytes. The sensor 22 is, for example, a biosensor coated with superoxide dismutase (SOD), and electrochemically measures active oxygen to obtain the number and concentration (cells/milliliter) of white blood cells. If the concentration of neutrophils in raw milk 2 is high, there is a possibility that mastitis has occurred.

The display unit 24 displays the amount of white blood cells (the number or concentration of white blood cells) based on the detection result of the sensor 22 .

Next, operation of the first embodiment will be described.

FIG. 2 shows the internal state of the container 12 and the component storage unit 13 before centrifugation in the first embodiment (FIG. 2(a)) and the internal state of the container 12 and the component storage unit 13 after centrifugation (FIG. 2 It is a figure which shows (b)). However, the screw 14 and shaft 15 are not shown.

First, the raw milk 2 is poured into the container 12 . Raw milk 2 fills the interior of container 12 and ingredient storage section 13 (see FIG. 2(a)). Here, when the motor 16 is started, the screw 14 is rotated, and the raw milk 2 is centrifuged, the milk fat content 2a is in the vicinity of the central axis A, the whey 2b is outside the container 12, and the component storage part further outside White blood cells 2c are separated within 13 (see FIG. 2(b)).

When the valve 17 is opened, the leukocytes 2c in the component containing portion 13 drop down in the pipe 11 and are given to the sensor 22. FIG. The sensor 22 detects white blood cells, acquires the number and concentration of white blood cells, and displays them on the display unit 24 .

According to the first embodiment, leukocytes can be detected without waiting for precipitation of leukocytes 2c in the centrifuged raw milk 2, so leukocytes can be detected in a short time.

Second Embodiment In the second embodiment, instead of the component storage portion 13 (see FIGS. 1 and 2) having a constant depth t, the vertical interval d becomes narrower as the distance from the central axis A increases. It differs from the first embodiment in that it includes a component storage section 18 (see FIGS. 3 and 4) and further includes a storage section 19 .

3A and 3B are diagrams showing the configuration of the white blood cell amount measuring device 1 according to the second embodiment of the present invention, including a plan view (FIG. 3A), a front view (FIG. 3B), and a side view ( FIG. 3(c)). However, in FIG. 3, the screw 14, the shaft 15 and the motor 16 are the same as those in the first embodiment (see FIG. 1) and are omitted from illustration.

A white blood cell amount measuring device 1 according to the second embodiment includes a centrifuge 10 , a sensor 22 and a display section 24 . Hereinafter, parts similar to those of the first embodiment are denoted by the same reference numerals, and descriptions thereof are omitted. The display unit 24 is the same as that of the first embodiment, and the explanation is omitted.

The centrifugal separator 10 includes a pipe 11 , a container 12 , a screw (rotating body) 14 , a shaft 15 , a motor 16 , a valve 17 , a component container 18 and a reservoir 19 . The container 12, the screw 14, the shaft 15, the motor 16 and the valve 17 are the same as those in the first embodiment, and their description is omitted.

The ingredient storage part 18 protrudes from the side surface 12S of the container 12 and accommodates the ingredients of the raw milk 2 (for example, white blood cells). However, the distance d in the vertical direction between the component storage portions 18 becomes narrower as the distance from the central axis A increases (see FIG. 3(b)).

The storage part 19 is provided in the part where the ingredient storage part 18 is farthest from the central axis A (also the part where the vertical distance d is the smallest), and stores the ingredients of the raw milk 2 (for example, white blood cells).

The pipe 11 is connected to the reservoir 19 .

The sensor 22 is connected to the pipe 11 and detects white blood cells (for example, neutrophils) stored in the reservoir 19 . More specifically, the sensor 22 receives the leukocytes stored in the reservoir 19 via the pipe 11 and detects the leukocytes. The sensor 22 is, for example, a biosensor coated with superoxide dismutase (SOD), and electrochemically measures active oxygen to obtain the number and concentration (cells/milliliter) of white blood cells. If the concentration of neutrophils in raw milk 2 is high, there is a possibility that mastitis has occurred.

Next, the operation of the second embodiment will be explained.

FIG. 4 shows the state of the inside of the container 12, the component storage portion 18 and the storage portion 19 before centrifugation (FIG. 4(a)) and the state of the container 12, the component storage portion 18 and the storage after centrifugation in the second embodiment. It is a figure which shows the state (FIG.4(b)) inside the part 19. FIG. However, the screw 14 and shaft 15 are not shown.

First, the raw milk 2 is poured into the container 12 . Raw milk 2 fills the inside of container 12, ingredient storage section 18 and storage section 19 (see FIG. 4(a)). Here, when the motor 16 is started, the screw 14 is rotated, and the raw milk 2 is centrifuged, the milk fat content 2a is in the vicinity of the central axis A, the whey 2b is outside the container 12, and the component storage part further outside White blood cells 2c are separated within 18 (see FIG. 4(b)). Since the vertical interval d is narrower on the outer side (the side away from the central axis A) of the ingredient storage section 18 , the concentration of the white blood cells 2 c is higher on the outer side, and the outermost storage section 19 is filled with white blood cells. , a high concentration of leukocytes 2c is stored.

When the valve 17 is opened, the leukocytes 2c in the reservoir 19 fall through the pipe 11 and are given to the sensor 22. FIG. The sensor 22 detects white blood cells, acquires the number and concentration of white blood cells, and displays them on the display unit 24 .

According to the second embodiment, the same effects as those of the first embodiment are obtained. Moreover, according to the second embodiment, the white blood cells are stored in the reservoir 19 at a high concentration due to the shape of the component storage part 18, so that the sensor 22 can easily detect the white blood cells.

1 white blood cell amount measuring device 10 centrifuge 11 pipe 12 container 12S side surface 12T top surface 12B bottom surface A central axis 13 component container t depth 14 screw (rotating body)
15 Shaft 16 Motor 17 Valve 18 Ingredient storage section d Vertical interval 19 Storage section 22 Sensor 24 Display section 2 Raw milk 2a Milk fat content 2b Whey 2c White blood cells

Claims (7)

a container containing raw milk;
a rotating body that is disposed inside the container and rotates about the central axis of the container;
an ingredient storage part that protrudes from the side surface of the container and stores ingredients of the raw milk;
with
the side surface intersects the bottom surface of the container;
the bottom surface intersects the central axis,
The vertical interval between the component storage portions becomes narrower as it moves away from the central axis,
A centrifugal separator in which a storage part for storing the component is arranged at a portion where the component storage part is most distant from the central axis . The centrifuge of claim 1 , wherein
A centrifugal separator, wherein the rotating body is a screw. The centrifuge of claim 1 , wherein
An adsorbent that adsorbs leukocytes in the raw milk is arranged in the component storage unit,
centrifuge. A centrifuge according to claim 3 , wherein
A centrifuge, wherein the adsorbent is P-selectin. The centrifuge of claim 1 , wherein
a pipe connected to the reservoir;
a valve that opens and closes the pipe;
Centrifuge with. a centrifuge according to any one of claims 1 to 5 ;
a sensor for detecting white blood cells obtained by the centrifuge;
a display unit that displays the amount of white blood cells based on the detection result of the sensor;
White blood cell amount measuring device with. a centrifuge according to claim 5 ;
a sensor that is connected to the pipe and detects white blood cells stored in the storage unit;
a display unit that displays the amount of white blood cells based on the detection result of the sensor;
White blood cell amount measuring device with.

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