JP7259019B2 - フローサイトメトリデータを分析するためのサイズに基づくゲーティング - Google Patents
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Description
Claims (16)
- 1つ以上のバイオマーカを発現する細胞の割合を定量する方法であって、
均質化試料を提供するために全腫瘍試料を均質化することと、
前記1つ以上のバイオマーカの存在に対して前記均質化試料中の細胞を染色することと、
サイズ及び粒度散乱に基づく前記均質化試料中の前記細胞の少なくとも第1の一次ゲーティングを実行して、第1の予測細胞型を有する少なくとも第1の細胞集団を提供することと、
前記第1の細胞集団内の第1の亜集団中の前記1つ以上のバイオマーカの1番目を発現する細胞の第1の割合を決定することと、
前記1つ以上のバイオマーカの2番目を発現する前記第1の細胞集団内の第2の亜集団中の第2の細胞の割合を更に決定することと、を含み、前記1つ以上のバイオマーカの前記1番目及び2番目が異なる、方法。 - 前記方法が、少なくとも第1の細胞亜集団中の第1の細胞の割合を決定する前に、物理選別工程を必要としない、請求項1に記載の方法。
- 前記第1の予測細胞型が、免疫細胞である、請求項1又は2に記載の方法。
- 前記免疫細胞が、約12μm未満のサイズである、請求項3に記載の方法。
- 前記1つ以上のバイオマーカの前記1番目を発現する前記第1の亜集団中の第1の細胞の割合が、前記1つ以上のバイオマーカの前記1番目の存在に基づき前記第1の亜集団中における前記細胞の二次ゲーティングを行うことによって決定される、請求項3に記載の方法。
- 前記1つ以上のバイオマーカの前記1番目が、分化バイオマーカのCD3、CD4、CD8、CD45RA、及びCD45ROクラスタからなる群から選択される、請求項5に記載の方法。
- 前記1つ以上のバイオマーカの前記2番目が、分化マーカのクラスタである、請求項1~6のいずれか一項に記載の方法。
- 前記1つ以上のバイオマーカのうちの前記2番目が、PD-1、TIM-3、LAG-3、CD28、CD57、及びFOXP3からなる群から選択される、請求項7に記載の方法。
- 前記1つ以上のバイオマーカの前記1番目が、CD3であり、前記1つ以上のバイオマーカの前記2番目が、調節性T細胞、ヘルパT細胞、又は細胞傷害性T細胞として前記免疫細胞を分化させるバイオマーカである、請求項1~6のいずれか一項に記載の方法。
- 前記均質化試料中の前記細胞の第2の一次ゲーティングを実行して、第2の予測細胞型を有する少なくとも第2の細胞集団を提供することを更に含む、請求項6に記載の方法。
- 前記第2の予測細胞型が、腫瘍細胞である、請求項10に記載の方法。
- 染色体異常を有する前記第2の細胞集団内の細胞の第3の割合を決定することを更に含む、請求項11に記載の方法。
- 前記染色体異常を有する前記決定された第3の細胞の割合を、前記1つ以上のバイオマーカの前記1番目を発現する細胞の前記決定された第1の割合と相関させることを更に含む、請求項12に記載の方法。
- 前記均質化試料が、染色の前に更に処理され、前記更なる処理が、前記均質化試料内のタンパク質を消化すること、前記均質化試料を加熱すること、又は前記均質化試料をフィルタにかけることの少なくとも1つを含む、請求項1~13のいずれか一項に記載の方法。
- 前記1つ以上のバイオマーカの前記1番目を発現する前記第1の亜集団中の前記決定された細胞の割合に部分的に基づいて治療決定を行うことを更に含む、請求項1に記載の方法。
- 1つ以上のバイオマーカを発現する細胞の割合を定量する方法であって、
前記1つ以上のバイオマーカの存在に対して試料中の細胞を染色することと、
サイズ及び粒度散乱に基づく前記試料中の前記細胞の少なくとも第1の一次ゲーティングを実行して、第1の予測細胞型を有する少なくとも第1の細胞集団を提供することと、
前記第1の細胞集団内の第1の亜集団中の前記1つ以上のバイオマーカの1番目を発現する細胞の第1の割合を決定することと、
前記1つ以上のバイオマーカの2番目を発現する前記第1の細胞集団内の第2の亜集団中の第2の細胞の割合を更に決定することと、を含み、前記1つ以上のバイオマーカの前記1番目及び2番目が異なる、方法。
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JP2015526709A (ja) | 2012-07-02 | 2015-09-10 | ソティオ エイ. エス. | 生体外におけるがんの診断及び監視方法 |
WO2017094008A1 (en) | 2015-12-01 | 2017-06-08 | The Medical Research, Infrastructure, And Health Services Fund Of The Tel Aviv Medical Center | Improved cytometric assays |
WO2018016656A1 (ja) | 2016-07-20 | 2018-01-25 | 一般社団法人 東京血液疾患研究所 | 骨髄異形成症候群の診断方法 |
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WO2017094008A1 (en) | 2015-12-01 | 2017-06-08 | The Medical Research, Infrastructure, And Health Services Fund Of The Tel Aviv Medical Center | Improved cytometric assays |
WO2018016656A1 (ja) | 2016-07-20 | 2018-01-25 | 一般社団法人 東京血液疾患研究所 | 骨髄異形成症候群の診断方法 |
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