JP7190266B2

JP7190266B2 - Diagnosis method for skin condition, method for estimating diversity of skin flora, method for estimating the amount of specific environment-derived bacteria, identification method based on the amount of specific environment-derived bacteria correlated with specific skin condition A method of analyzing the attributes of bacteria that are correlated with skin conditions, a method of screening substances or cosmetic methods that have an action to improve skin conditions

Info

Publication number: JP7190266B2
Application number: JP2018116436A
Authority: JP
Inventors: 薫笠原; 宏大島
Original assignee: Pola Chemical Industries Inc
Current assignee: Pola Chemical Industries Inc
Priority date: 2018-06-19
Filing date: 2018-06-19
Publication date: 2022-12-15
Anticipated expiration: 2038-06-19
Also published as: JP2019216652A

Description

口腔の細菌叢が様々な疾患と関連を有することが近年報告されている。たとえば、特許文献１には、細菌叢における菌種の多様性を身体状態の指標とする技術が開示されている。 In recent years, it has been reported that the oral flora is associated with various diseases. For example, Patent Literature 1 discloses a technique in which the diversity of bacterial species in bacterial flora is used as an indicator of physical condition.

また、特許文献２、３にあるように、特定の菌種と肌状態の関係についても従来知られている。 Moreover, as disclosed in Patent Documents 2 and 3, the relationship between specific bacterial strains and skin conditions has been conventionally known.

特開２０１７－２９１３３号公報JP 2017-29133 A 特開２０１７－２０９０５５号公報JP 2017-209055 A 特表２０１６－５２８８８０号公報Japanese translation of PCT publication No. 2016-528880

前述の特許文献１に記載の技術では、多様性のみを指標とし、皮膚細菌叢中の菌の種類を問わないため、肌状態の診断結果にブレが生じることが課題としてあった。
そのため、皮膚常在細菌叢を利用し行われる肌状態の診断方法において、より効率よくかつ的確に肌状態を診断する技術が求められていた。 In the technique described in Patent Document 1 mentioned above, only diversity is used as an index, and the type of bacteria in the skin flora is not considered.
Therefore, there has been a demand for a technique for more efficiently and accurately diagnosing skin conditions in methods of diagnosing skin conditions using indigenous skin flora.

肌状態をより効率よくかつ的確に診断するための指標を求め鋭意研究に励んだ結果、肌状態が良好な被験者の細菌叢に存在する菌の種類に偏りがあることを見出し、本発明を完成させた。 As a result of diligent research in search of an index for diagnosing skin conditions more efficiently and accurately, it was found that there was a bias in the types of bacteria present in the bacterial flora of subjects with good skin conditions, and the present invention was completed. let me

すなわち、本発明は、
特定の肌状態の診断方法であって、
皮膚常在細菌叢に存在する菌の菌種を同定する菌種同定工程と、
同定した菌種と、環境由来菌情報と、に基づき前記皮膚常在細菌叢における前記特定の肌状態と相関のある特定の環境由来菌の菌量を計測する計測工程と、
計測結果に基づき、前記特定の環境由来菌の菌量が多いほど、前記特定の肌状態が好ましいと判定する判定工程と、
を有し、
前記環境由来菌情報は、菌種又は菌属に関する情報と、その菌種又は菌属の検出された環境に関する情報と、を紐づけた情報であることを特徴とする、診断方法である。 That is, the present invention
A method for diagnosing a specific skin condition, comprising:
a bacterial species identification step of identifying bacterial species present in the skin flora;
a measurement step of measuring the amount of specific environment-derived bacteria correlated with the specific skin condition in the indigenous skin flora based on the identified bacterial species and information on the environment-derived bacteria;
a determination step of determining that the specific skin condition is more favorable as the amount of the specific environment-derived bacteria increases, based on the measurement results;
has
The diagnostic method is characterized in that the environment-derived bacterial information is information in which information relating to a bacterial species or genus is associated with information relating to the environment in which the bacterial species or genus is detected.

本発明によれば、特定の肌状態と相関のある特定の環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、肌状態を診断することができる。 According to the present invention, the skin condition can be diagnosed noninvasively without directly examining the skin based on the amount of specific environment-derived bacteria that correlates with the specific skin condition.

本発明の好ましい形態では、
前記特定の肌状態がＴＥＷＬ（経皮水分蒸散量）のパラメータに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＡ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ａ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［animal associated］、［animal body associated］、［land feature］ In a preferred form of the invention,
the specific skin condition is based on a TEWL (transepidermal water loss) parameter;
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group A defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group A)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [animal associated], [animal body associated], [land feature]

本発明の好ましい形態によれば、上記Ａ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, a parameter of TEWL (transepidermal water loss) can be obtained non-invasively without directly examining the skin based on the amount of environment-derived bacteria derived from the environment selected from the above group A. It is possible to diagnose skin conditions based on

また、本発明の好ましい形態では、
前記特定の肌状態が皮膚の明るさ（Ｌ^＊）のパラメータに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＢ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｂ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［land feature］ Also, in a preferred form of the invention,
wherein the specific skin condition is based on a skin lightness (L ^* ) parameter;
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group B defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group B)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [land feature]

本発明の好ましい形態によれば、上記Ｂ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮膚の明るさ（Ｌ^＊）のパラメータに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the skin brightness (L ^* ) parameter can be obtained noninvasively without directly examining the skin, based on the amount of environment-derived bacteria selected from the above group B. It is possible to diagnose skin conditions based on

また、本発明の好ましい形態では、
前記特定の肌状態が肌の弾力性（Ｕｒ／Ｕｆ）のパラメータに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＣ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｃ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［park associated］、［land area］ Also, in a preferred form of the invention,
wherein the specific skin condition is based on a skin elasticity (Ur/Uf) parameter;
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group C defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group C)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [park associated], [land area]

本発明の好ましい形態によれば、上記Ｃ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、肌の弾力性（Ｕｒ／Ｕｆ）のパラメータに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the skin elasticity (Ur/Uf) can be measured non-invasively without directly examining the skin, based on the amount of environment-derived bacteria selected from the above group C. A skin condition can be diagnosed based on parameters.

また、本発明の好ましい形態では、
前記特定の肌状態がシミの少なさに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＤ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｄ群）
［plant body associated 若しくは symbiotic microbe］、［water associated］、［animal associated］、［animal body associated］、［land feature］ Also, in a preferred form of the invention,
The specific skin condition is based on less spots,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group D defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group D)
[plant body associated or symbiotic microbe], [water associated], [animal associated], [animal body associated], [land feature]

本発明の好ましい形態によれば、上記Ｄ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、シミの少なさに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria selected from the above group D is used to non-invasively diagnose the skin condition based on the amount of blemishes without directly examining the skin. can do.

また、本発明の好ましい形態では、
前記特定の肌状態が角層水分量の多さに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＥ群から選ばれる環境に由来する環境由来菌のうち、１又は２であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｅ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］ Also, in a preferred form of the invention,
The specific skin condition is based on a large stratum corneum moisture content,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group E defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group E)
[plant body associated or symbiotic microbe], [agriculture associated]

本発明の好ましい形態によれば、上記Ｅ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、角層水分量の多さに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above group E is used to non-invasively measure the skin based on the amount of water in the stratum corneum without directly examining the skin. condition can be diagnosed.

また、本発明の好ましい形態では、
前記特定の肌状態が肌の黄味の少なさに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される［plant body associated 若しくは symbiotic microbe］に由来する環境由来菌であることを特徴とする。 Also, in a preferred form of the invention,
The specific skin condition is based on less yellowness of the skin,
The specific environment-derived bacteria are environment-derived bacteria derived from [plant body associated or symbiotic microbe] defined in the National Institute of Genetics bacteria database (MicrobeDB.jp). do.

本発明の好ましい形態によれば、上記環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、肌の黄味の少なさに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, it is possible to non-invasively diagnose the skin condition based on the low yellowness of the skin, without directly examining the skin, based on the amount of the environment-derived bacteria.

また、本発明の好ましい形態では、
前記特定の肌状態が頬部の凹凸の少なさに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＦ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｆ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［park associated］、［land area］、［land feature］、［fungi body associated］ Also, in a preferred form of the invention,
The specific skin condition is based on less unevenness of the cheeks,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group F defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(F group)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [animal body associated], [park associated], [ land area], [land feature], [fungi body associated]

本発明の好ましい形態によれば、上記Ｆ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、頬部の凹凸の少なさに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above group F is used to non-invasively measure the skin based on the small unevenness of the cheek without directly examining the skin. condition can be diagnosed.

また、本発明の好ましい形態では、
前記特定の肌状態が目立つ毛穴個数の少なさに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＧ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｇ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［park associated］、［land area］、［land feature］ Also, in a preferred form of the invention,
It is based on the small number of pores that make the specific skin condition conspicuous,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following G groups defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group G)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [animal body associated], [park associated], [ land area], [land feature]

本発明の好ましい形態によれば、上記Ｇ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、目立つ毛穴個数の少なさに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the skin condition is based on the number of conspicuous pores noninvasively without directly examining the skin, based on the amount of bacteria derived from the environment selected from the above group G. can be diagnosed.

また、本発明の好ましい形態では、
前記特定の肌状態が紅斑指数の低さに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＨ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｈ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］ Also, in a preferred form of the invention,
The specific skin condition is based on a low erythema index,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following H group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group H)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [animal body associated], [land feature]

本発明の好ましい形態によれば、上記Ｈ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、紅斑指数の低さに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the skin condition based on a low erythema index can be determined non-invasively without directly examining the skin, using the amount of environment-derived bacteria selected from the above group H. can be diagnosed.

また、本発明の好ましい形態では、
前記特定の肌状態がキメ状態の良さに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＩ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｉ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［land area］、［land feature］、［fungi body associated］ Also, in a preferred form of the invention,
The specific skin condition is based on a good texture condition,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group I defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group I)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [land area], [land feature], [fungi body associated]

本発明の好ましい形態によれば、上記Ｉ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、キメ状態の良さに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the skin condition is non-invasively diagnosed based on the good texture condition without directly examining the skin, based on the amount of environment-derived bacteria selected from the above group I. can do.

また、本発明の好ましい形態では、
前記特定の肌状態が皮脂量の少なさに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＪ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｊ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［animal associated］、［animal body associated］、［land area］、［land feature］ Also, in a preferred form of the invention,
The specific skin condition is based on a small amount of sebum,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group J defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(Group J)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [animal associated], [animal body associated], [land area], [land feature]

本発明の好ましい形態によれば、上記Ｊ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮脂量の少なさに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above J group is used to non-invasively evaluate the skin condition based on the low sebum content without directly examining the skin. can be diagnosed.

また、本発明の好ましい形態では、
前記特定の肌状態がシワ本数の少なさに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＫ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｋ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［animal associated］、［animal body associated］、［land feature］ Also, in a preferred form of the invention,
The specific skin condition is based on a small number of wrinkles,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following K groups defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(K group)
[plant body associated or symbiotic microbe], [agriculture associated], [animal associated], [animal body associated], [land feature]

本発明の好ましい形態によれば、上記Ｋ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、シワ本数の少なさに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the skin condition based on the low number of wrinkles can be determined non-invasively without directly examining the skin, using the amount of environment-derived bacteria selected from the above K group. can be diagnosed.

また、本発明の好ましい形態では、
前記特定の肌状態がメラニン指数の低さに基づくものであり、
前記特定の環境由来菌は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＬ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上であることを特徴とする。
（なお、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるという。）
（Ｌ群）
［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］ Also, in a preferred form of the invention,
The specific skin condition is based on a low melanin index,
The specific environment-derived bacteria are 1 or 2 of the environment-derived bacteria selected from the following group L defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is characterized by the above.
(Of the environments in which the bacterium or the genus to which the bacterium belongs, the environment with the highest average abundance ratio of the bacterium or the genus to which the bacterium belongs is referred to as the environment from which the bacterium originates.)
(L group)
[agriculture associated], [water associated], [animal associated], [animal body associated], [land feature]

本発明の好ましい形態によれば、上記Ｌ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、メラニン指数の低さに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the skin condition based on a low melanin index can be determined non-invasively without directly examining the skin, using the amount of environment-derived bacteria selected from the above group L. can be diagnosed.

また、本発明は、
皮膚常在細菌叢の多様性の推測方法であって、
皮膚常在細菌叢に存在する菌の菌種を同定する菌種同定工程と、
同定した菌種と、環境由来菌情報と、に基づき前記皮膚常在細菌叢における特定の肌状態と相関のある特定の環境由来菌の菌量を計測する計測工程と、
計測結果に基づき、前記特定の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると判定する判定工程と、を有し、
前記環境由来菌情報は、菌種又は菌属に関する情報と、その菌種又は菌属の検出された環境に関する情報と、を紐づけた情報であり、
前記特定の肌状態は、前記多様性と相関のある肌状態であることを特徴とする、推測方法でもある。 In addition, the present invention
A method for estimating the diversity of skin flora, comprising:
a bacterial species identification step of identifying bacterial species present in the skin flora;
a measurement step of measuring the amount of specific environment-derived bacteria in the indigenous skin flora that correlates with a specific skin condition based on the identified bacterial species and information on the environment-derived bacteria;
a determination step of determining, based on the measurement results, that the greater the amount of the specific environment-derived bacteria, the more diverse the indigenous skin microflora;
The environment-derived bacterium information is information that links information on the bacterium species or genus with information on the environment in which the bacterium species or genus is detected,
It is also an estimation method, characterized in that the specific skin condition is a skin condition correlated with the diversity.

本発明によれば、特定の肌状態と相関のある特定の環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮膚常在細菌叢の多様性を推測することができる。 INDUSTRIAL APPLICABILITY According to the present invention, it is possible to non-invasively estimate the diversity of the indigenous skin microflora without directly examining the skin, based on the amount of specific environment-derived bacteria that are correlated with a specific skin condition. can.

また、本発明は、
皮膚常在細菌叢における多様性を解析する多様性解析工程と、
解析結果に基づき、前記多様性に富んでいるほど、前記皮膚常在細菌叢における特定の肌状態と相関のある特定の環境由来菌の菌量が多いと判定する判定工程と、
を有し、
前記特定の肌状態は、前記多様性と相関のある肌状態であることを特徴とする、皮膚常在細菌叢における前記特定の環境由来菌の菌量の推測方法でもある。 In addition, the present invention
A diversity analysis step of analyzing diversity in skin flora,
a determination step of determining, based on the analysis results, that the greater the diversity, the greater the amount of specific environment-derived bacteria correlated with a specific skin condition in the indigenous skin flora;
has
The specific skin condition is also a method for estimating the amount of the specific environment-derived bacteria in the indigenous skin flora, characterized in that the specific skin condition is a skin condition that is correlated with the diversity.

本発明によれば、皮膚常在細菌叢における多様性によって、直接肌を検査することなく、非侵襲的に、特定の肌状態と相関のある特定の環境由来菌の菌量を推測することができる。 According to the present invention, it is possible to noninvasively estimate the amount of specific environment-derived bacteria that are correlated with a specific skin condition, without directly examining the skin, based on the diversity of the indigenous skin flora. can.

また、本発明は、
特定の肌状態と相関のある菌の属性を解析する方法であって、
前記特定の肌状態の良い被験者群と悪い被験者群の皮膚常在細菌叢に存在する菌において、両群間で菌量に差のある菌種又は菌属を特定する特定工程と、
前記特定工程で特定された菌種又は菌属の属性に関する情報を取得することを特徴とする、解析方法でもある。 In addition, the present invention
A method for analyzing the attributes of bacteria correlated with a specific skin condition,
an identifying step of identifying the species or genus of bacteria present in the indigenous skin flora of the specific group of subjects with good skin condition and the group of subjects with poor skin condition, which have a difference in the amount of bacteria between the two groups;
It is also an analysis method characterized by acquiring information on the attributes of the bacterial species or genus identified in the identifying step.

また、本発明の好ましい形態では、
前記属性が、前記特定工程で特定された菌の由来環境であることを特徴とする。 Also, in a preferred form of the invention,
The attribute is characterized by being the origin environment of the bacterium identified in the identification step.

また、本発明は、肌状態の改善作用を有する物質又は美容方法のスクリーニング方法であって、
特定の肌状態と相関のある特定の環境由来菌の菌量が増えるほど、肌状態の改善作用に優れると判定することを特徴とする、肌状態の改善作用を有する物質又は美容方法のスクリーニング方法でもある。
本発明によれば、特定の肌状態と相関のある特定の環境由来菌の菌量に依って、直接肌を検査することなく、非侵襲的に、肌状態の改善作用を有する物質又は美容方法をスクリーニングすることができる。 The present invention also provides a screening method for a substance or cosmetic method that has an effect of improving skin conditions,
A screening method for a substance or cosmetic method having a skin condition-improving effect, characterized in that the greater the amount of specific environment-derived bacteria correlated with a specific skin condition, the more excellent the skin condition-improving effect. But also.
According to the present invention, there is provided a substance or cosmetic method that noninvasively improves skin conditions without directly examining the skin, depending on the amount of specific environment-derived bacteria that are correlated with specific skin conditions. can be screened.

国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される、「Environmental composition of Meta 16s」のリストデータを示す参考図である。Fig. 2 is a reference diagram showing list data of "Environmental composition of Meta 16s" defined in the National Institute of Genetics bacterial database (MicrobeDB.jp). ＴＥＷＬ（経皮水分蒸散量）の良好な被験者群に存在する環境由来菌の菌量を示すグラフである。1 is a graph showing the amount of environment-derived bacteria present in a group of subjects with good TEWL (transepidermal water loss). 皮膚の明るさ（Ｌ^＊）の良好な被験者群に存在する環境由来菌の菌量を示すグラフである。1 is a graph showing the amount of environment-derived bacteria present in a group of subjects with good skin brightness (L ^* ). 肌の弾力性（Ｕｒ／Ｕｆ）の良好な被験者群に存在する環境由来菌の菌量を示すグラフである。4 is a graph showing the amount of environment-derived bacteria present in a group of subjects with good skin elasticity (Ur/Uf). シミの少ない被験者群に存在する環境由来菌の菌量を示すグラフである。4 is a graph showing the amount of environment-derived bacteria present in a test subject group with few spots. 角層水分量の多い被験者群に存在する環境由来菌の菌量を示すグラフである。2 is a graph showing the amount of environment-derived bacteria present in a subject group with a high stratum corneum moisture content. 黄味の少ない被験者群に存在する環境由来菌の菌量を示すグラフである。FIG. 10 is a graph showing the amount of environment-derived bacteria present in a subject group with little yellowness. FIG. 頬部の凹凸の少ない被験者群に存在する環境由来菌の菌量を示すグラフである。4 is a graph showing the amount of environment-derived bacteria present in a test subject group with less uneven cheeks. 目立つ毛穴個数の少ない被験者群に存在する環境由来菌の菌量を示すグラフである。4 is a graph showing the amount of environment-derived bacteria present in a subject group with a small number of conspicuous pores. 紅斑指数の低い被験者群に存在する環境由来菌の菌量を示すグラフである。4 is a graph showing the amount of environment-derived bacteria present in a group of subjects with a low erythema index. キメ状態の良い被験者群に存在する環境由来菌の菌量を示すグラフである。2 is a graph showing the amount of environment-derived bacteria present in a group of subjects with good skin texture. 皮脂量の少ない被験者群に存在する環境由来菌の菌量を示すグラフである。4 is a graph showing the amount of environment-derived bacteria present in a subject group with a small amount of sebum. シワ本数の少ない被験者群に存在する環境由来菌の菌量を示すグラフである。4 is a graph showing the amount of environment-derived bacteria present in a subject group with a small number of wrinkles. メラニン指数の低い被験者群に存在する環境由来菌の菌量を示すグラフである。4 is a graph showing the amount of environment-derived bacteria present in a test subject group with a low melanin index. 目立つ毛穴個数の少ない被験者群と、目立つ毛穴個数の多い被験者群との間の、皮膚常在細菌叢における多様性を比較したグラフである。FIG. 10 is a graph comparing the diversity in the resident skin flora between a subject group with a small number of conspicuous pores and a subject group with a large number of conspicuous pores. FIG. 皮脂量の少ない被験者群と、皮脂量の多い被験者群との間の、皮膚常在細菌叢における多様性を比較したグラフである。1 is a graph comparing the diversity in the resident skin flora between a group of subjects with low sebum content and a group of subjects with high sebum content. 紅斑指数の低い被験者群と、紅斑指数の高い被験者群との間の、皮膚常在細菌叢における多様性を比較したグラフである。Fig. 3 is a graph comparing the diversity in the resident skin flora between a group of subjects with a low erythema index and a group of subjects with a high erythema index. ＴＥＷＬ（経皮水分蒸散量）の良好な被験者群と、ＴＥＷＬ（経皮水分蒸散量）の悪い被験者群との間の、皮膚常在細菌叢における多様性を比較したグラフである。Fig. 3 is a graph comparing the diversity in the resident skin flora between a group of subjects with good TEWL (transepidermal water loss) and a group of subjects with poor TEWL (transepidermal water loss). メラニン指数の低い被験者群と、メラニン指数の高い被験者群との間の、皮膚常在細菌叢における多様性を比較したグラフである。Fig. 3 is a graph comparing the diversity in the resident skin flora between a group of subjects with low melanin index and a group of subjects with high melanin index. 皮膚の明るさ（Ｌ^＊）の良好な被験者群と、皮膚の明るさ（Ｌ^＊）の悪い被験者群との間の、皮膚常在細菌叢における多様性を比較したグラフである。FIG. 2 is a graph comparing the diversity in the resident skin flora between a group of subjects with good skin lightness (L ^* ) and a group of subjects with poor skin lightness (L ^* ).

＜１＞肌状態の診断方法
以下、本発明の肌状態の診断方法における、好ましい実施形態について説明するが、本発明の技術的範囲は以下の実施形態に限定されないことは言うまでもない。 <1> Skin Condition Diagnosis Method Preferred embodiments of the skin condition diagnosis method of the present invention will be described below, but it goes without saying that the technical scope of the present invention is not limited to the following embodiments.

本発明の診断方法は、
皮膚常在細菌叢に存在する菌の菌種を同定する菌種同定工程と、
同定した菌種と、環境由来菌情報と、に基づき皮膚常在細菌叢における特定の肌状態と相関のある特定の環境由来菌の菌量を計測する計測工程と、
計測結果に基づき、特定の環境由来菌の菌量が多いほど、特定の肌状態が好ましいと判定する判定工程と、を有することを特徴とする。
ここで、環境由来菌情報は、菌種又は菌属に関する情報と、その菌種又は菌属の検出された環境に関する情報と、を紐づけた情報であることを特徴とする。 The diagnostic method of the present invention comprises
a bacterial species identification step of identifying bacterial species present in the skin flora;
a measurement step of measuring the amount of specific environment-derived bacteria in the indigenous skin flora that correlates with a specific skin condition based on the identified bacterial species and the information on the environment-derived bacteria;
and a determination step of determining that the specific skin condition is more favorable as the amount of specific environment-derived bacteria increases, based on the measurement result.
Here, the environment-derived bacterium information is characterized by linking information on the bacterium species or genus with information on the environment in which the bacterium species or genus is detected.

以下、本実施例の診断方法の各工程について、詳細に説明する。 Each step of the diagnostic method of the present embodiment will be described in detail below.

（１）菌種同定工程
本発明の実施形態において、菌種同定工程は皮膚常在細菌叢に存在する菌の菌種を同定する工程である。 (1) Bacterial species identification step In an embodiment of the present invention, the bacterial species identification step is a step of identifying the species of bacteria present in the indigenous skin flora.

皮膚常在細菌叢を採取する方法は、皮膚常在細菌叢を採取することができれば特に制限はないが、Ｓｗａｂ法を好ましく例示できる。 The method for collecting the resident skin flora is not particularly limited as long as the resident skin flora can be collected, but the Swab method can be preferably exemplified.

採取した皮膚常在細菌叢を解析する方法としては、皮膚常在細菌叢における細菌の様態について解析できる方法であれば特に制限はなく、免疫学的手法、質量分析法、ＰＣＲ法、ＤＮＡシークエンスなどを利用する方法が挙げられるが、特に好ましくは次世代シークエンサーを用いた１６ＳｒＲＮＡ系統解析を好ましく例示できる。 The method for analyzing the collected skin flora is not particularly limited as long as it is a method that can analyze the state of bacteria in the skin flora, such as immunological methods, mass spectrometry, PCR, and DNA sequencing. , but particularly preferably 16S rRNA phylogenetic analysis using a next-generation sequencer can be exemplified.

１６ＳｒＲＮＡ系統解析は、次世代シークエンサーを用いてサンプル中に含まれる細菌種の同定を行う解析手法である。当該解析においては、まず、サンプルからＤＮＡを抽出し、リボソーム１６Ｓサブユニット領域をＰＣＲで増幅した後、次世代シークエンサーを用いて高速かつ大量にアンプリコンのＤＮＡ配列を読み取る。その後、低クオリティリードやキメラ配列の除去を行った後、配列同士をクラスタリングしてＯＴＵ（ＯｐｅｒａｔｉｏｎａｌＴａｘｏｎｏｍｉｃＵｎｉｔ）解析を行う。ＯＴＵとは、ある一定以上の類似性（一般的には９６－９７％）を持つ配列同士を一つの菌種のように扱うための操作上の分類単位である。そして、各ＯＴＵのＤＮＡ配列を菌のＤＮＡ配列データベースと照合することにより、属種名の同定を行うことができる。 16S rRNA phylogenetic analysis is an analysis technique that uses a next-generation sequencer to identify bacterial species contained in a sample. In the analysis, DNA is first extracted from the sample, the ribosomal 16S subunit region is amplified by PCR, and then the DNA sequence of the amplicon is read at high speed and in large quantities using a next-generation sequencer. Then, after removing low-quality reads and chimeric sequences, the sequences are clustered and OTU (Operational Taxonomic Unit) analysis is performed. An OTU is an operational taxonomic unit for treating sequences having a certain level of similarity (generally 96-97%) as if they were a single fungal species. Then, the genus species name can be identified by matching the DNA sequence of each OTU with the bacterial DNA sequence database.

また、ＯＴＵ数はサンプル中に含まれる菌叢を構成する菌種の数を表し、同一のＯＴＵに属するリードの数はその種の相対的な存在量を表す。したがって、同一のＯＴＵに属するリードの数に基づき、サンプルに含まれる細菌叢における特定の菌種の占有率を算出することができる。 In addition, the number of OTUs represents the number of bacterial species that constitute the bacterial flora contained in the sample, and the number of reads belonging to the same OTU represents the relative abundance of that species. Therefore, based on the number of reads belonging to the same OTU, it is possible to calculate the occupancy rate of a particular bacterial species in the bacterial flora contained in the sample.

さらに、それぞれの菌種の占有率のデータに基づき、たとえばＳｉｍｐｓｏｎＩｎｄｅｘやＳｈａｎｎｏｎｄｉｖｅｒｓｉｔｙｉｎｄｅｘなどの指数を算出することにより、皮膚常在細菌叢の多様性も算出することができる。 Furthermore, by calculating an index such as the Simpson Index or the Shannon diversity index based on the data on the occupancy rate of each bacterial species, the diversity of the indigenous skin flora can also be calculated.

ＯＴＵ解析に基づく、皮膚常在細菌叢に存在する特定の菌種の占有率の算出も、皮膚常在細菌叢の多様性の算出も、市販の１６ＳｒＲＮＡ系統解析ソフトにより容易に行うことができる。 Both the calculation of the occupancy of specific bacterial species present in the skin flora and the diversity of the skin flora based on the OTU analysis can be easily performed using commercially available 16S rRNA phylogenetic analysis software.

なお、１６ＳｒＲＮＡ系統解析により皮膚常在細菌叢に存在する特定の菌種の解析を行う場合であっても、皮膚常在細菌叢に存在する特定の菌種の占有率の算出、並びに、皮膚常在細菌叢の多様性の算出はＯＴＵ解析以外の手法によって行ってもよい。 In addition, even when analyzing a specific bacterial species present in the skin flora by 16S rRNA phylogenetic analysis, calculation of the occupancy rate of a specific bacterial species present in the skin flora, Calculation of the diversity of bacterial flora may be performed by a method other than the OTU analysis.

（２）計測工程
計測工程は、同定した菌種と、環境由来菌情報と、に基づき皮膚常在細菌叢における特定の肌状態と相関のある特定の環境由来菌の菌量を計測する工程である。 (2) Measurement process The measurement process is a process of measuring the amount of specific environment-derived bacteria that correlates with a specific skin condition in the skin flora based on the identified bacterial species and information on the environment-derived bacteria. be.

計測工程は環境由来菌情報を備えるデータベースに同定した菌種を照合する工程を含む形態とすることがより好ましい。 More preferably, the measuring step includes a step of collating the identified bacterial species with a database containing information on environment-derived bacteria.

また、計測工程は環境由来菌情報を備えるデータベースが備える検索欄に同定した菌種を記入する工程を含む形態とすることがさらに好ましい。 Further, it is more preferable that the measuring step include a step of entering the identified bacterial species in a search field provided in a database having information on environment-derived bacteria.

また、環境由来菌情報を備えるデータベースは、各細菌の環境中での平均存在比率に関する情報を有するデータベースであることが好ましい。ここで、各細菌の環境中での平均存在比率は、既に報告された文献情報をメタ解析することにより算出されたものであることが、より好ましい。 Moreover, it is preferable that the database containing information on environment-derived bacteria is a database having information on the average abundance ratio of each bacterium in the environment. Here, it is more preferable that the average abundance ratio of each bacterium in the environment is calculated by meta-analyzing previously reported literature information.

また、環境由来菌情報を備えるデータベースは、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）であることがさらに好ましい。 Further, it is more preferable that the database provided with information on environment-derived bacteria is the bacteria database (MicrobeDB.jp) of the National Institute of Genetics.

本発明において、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される、「Environmental composition of Meta 16s」のリストデータのうち、最上位の環境をその細菌の由来環境とすることがより好ましい（図１参照）。 In the present invention, among the list data of "Environmental composition of Meta 16s" defined in the bacterium database (MicrobeDB.jp) of the National Institute of Genetics, the top environment is the origin environment of the bacterium. It is more preferable to set (see Fig. 1).

また、本明細書において、「特定の肌状態と相関のある特定の環境由来菌の菌量を計測する」とは、同一の由来環境の菌の総量を計測することをいう。 Further, in the present specification, "to measure the amount of bacteria derived from a specific environment that is correlated with a specific skin condition" means to measure the total amount of bacteria originating from the same environment.

また、本発明でいう環境由来菌情報は、菌種又は菌属に関する情報と、その菌種又は菌属の検出された環境に関する情報と、を紐づけた情報である。 In addition, the environment-derived bacteria information as used in the present invention is information in which information relating to a bacterial species or genus is linked to information relating to the environment in which the bacterial species or genus is detected.

環境由来菌情報は、菌種又は菌属に関する情報と、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される菌の由来環境であって、菌が検出された環境のうち該菌の平均存在比率の最も高い環境に関する情報とを、紐づけた情報であることが好ましい。 Environment-derived bacteria information includes information on the species or genus of bacteria, and the environment of origin of the bacteria specified in the bacteria database (MicrobeDB.jp) of the National Institute of Genetics (National Institute of Genetics). It is preferable that the information is linked with information related to the environment having the highest average abundance ratio of the bacteria among the environments.

本発明においては、２以上の環境由来菌の菌量を計測する形態とすることが好ましい。
このような形態とすることで、より効率よく肌状態を診断することができる。 In the present invention, it is preferable to adopt a mode in which the amount of two or more environment-derived bacteria is measured.
By adopting such a form, the skin condition can be diagnosed more efficiently.

（３）判定工程
判定工程は、計測結果に基づき、特定の環境由来菌の菌量が多いほど、特定の肌状態が好ましいと判定する工程である。 (3) Judgment Step The judgment step is a step for judging that the specific skin condition is more favorable as the amount of specific environment-derived bacteria increases, based on the measurement results.

ここで、特定の環境由来菌の菌量が多いほど、特定の肌状態が好ましいと判定するための方法は特に限定されない。 Here, there is no particular limitation on the method for determining that the greater the amount of specific environment-derived bacteria, the more favorable the specific skin condition.

たとえば、統計学的に有意な人数に対して、従来の手法による肌状態の診断と、皮膚常在細菌叢の解析を行うことで、皮膚常在細菌叢に存在する特定の菌の菌量と肌状態の相関についてのデータベースを予め作成しておき、被験者の皮膚常在細菌叢に存在する特定の菌の菌量を当該データベースと照合することにより被験者の肌状態を診断する実施の形態が好ましく挙げられる。 For example, for a statistically significant number of people, by diagnosing the skin condition by conventional methods and analyzing the skin flora, the amount and amount of specific bacteria present in the skin flora A preferable embodiment is one in which a database of skin condition correlations is created in advance, and the skin condition of a subject is diagnosed by comparing the amount of specific bacteria present in the skin flora of the subject with the database. mentioned.

複数の被験者の皮膚常在細菌叢に存在する特定の菌の菌量に基づき、被験者間での肌状態の優劣を診断する実施の形態としてもよい。
また、同一被験者から取得した複数の皮膚常在細菌叢に存在する特定の菌の菌量に基づき、相対的に肌状態を診断する実施の形態としてもよい。
また、被験者から取得した皮膚常在細菌叢を、既に用意された指標と照らし合わせることにより、肌状態を診断する実施の形態としてもよい。 It is also possible to adopt an embodiment of diagnosing the superiority or inferiority of the skin condition among subjects based on the amount of specific bacteria present in the indigenous skin flora of a plurality of subjects.
Moreover, it is good also as an embodiment which diagnoses a skin condition relatively based on the bacterial quantity of the specific microbe which exists in the skin microflora of several obtained from the same test subject.
In addition, an embodiment may be employed in which the skin condition is diagnosed by comparing the indigenous skin microflora acquired from the subject with an index that has already been prepared.

上述の通り、皮膚常在細菌叢における前記特定の肌状態と相関のある特定の環境由来菌の菌量を指標とし、肌状態を診断する。 As described above, the skin condition is diagnosed using the amount of specific environment-derived bacteria in the indigenous skin flora that correlates with the specific skin condition as an index.

以下、本発明の診断方法における、好ましい実施の形態について詳述する。 Preferred embodiments of the diagnostic method of the present invention are described in detail below.

本発明の実施の形態では、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＡ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ａ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［animal associated］、［animal body associated］、［land feature］ In an embodiment of the present invention, one or two of the environment-derived bacteria derived from the environment selected from the following group A defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is preferable to determine that the skin condition based on the TEWL (transepidermal water loss) parameter is more favorable as the amount of the above environment-derived bacteria increases.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(Group A)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [animal associated], [animal body associated], [land feature]

本発明の好ましい実施の形態によれば、上記Ａ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, TEWL (transepidermal water loss) can be measured non-invasively without directly examining the skin, based on the amount of environment-derived bacteria derived from the environment selected from the above group A. It is possible to diagnose the skin condition based on the parameters of

本発明の実施の形態では、Ａ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably all environment-derived bacteria selected from the environment selected from Group A It is preferable to determine that the skin condition based on the parameter of TEWL (transepidermal water loss) is more favorable as the amount of bacteria derived from the skin increases.

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［animal body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態が好ましいと判定する形態とすることがより好ましい。 In an embodiment of the present invention, one or two or more environment-derived bacteria among environment-derived bacteria derived from environments selected from [plant body associated or symbiotic microbe], [agriculture associated], and [animal body associated] environments It is more preferable to determine that the skin condition based on the TEWL (transepidermal water loss) parameter is more favorable as the amount of bacteria in the skin increases.

また、［aquatic area 若しくは fluid in state］及び／又は［animal associated］の環境に由来する環境由来菌の菌量が、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［animal body associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の３分の１以下であるときに、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, the amount of environment-derived bacteria derived from the environment of [aquatic area or fluid in state] and / or [animal associated] is [plant body associated or symbiotic microbe], [agriculture associated], [animal body associated], Among the environment-derived bacteria derived from the environment selected from the [land feature] environment, when the amount of each of 1 or 2 or more environment-derived bacteria is less than one-third, TEWL (transepidermal water loss) ) is more preferable.

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［animal body associated］の環境から選ばれる環境に由来する環境由来菌のうち、２以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態が好ましいと判定することが好ましい。 In an embodiment of the present invention, two or more, more preferably all, environment-derived bacteria derived from environments selected from [plant body associated or symbiotic microbe], [agriculture associated], and [animal body associated] environments It is preferable to determine that the skin condition based on the TEWL (transepidermal water loss) parameter is more favorable as the amount of bacteria derived from the environment increases.

本発明は、Ａ群のうち、好ましくは［plant body associated 若しくは symbiotic microbe］の環境に由来する菌の菌量が多いほど、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In the present invention, the skin condition based on the parameter of TEWL (transepidermal water loss) is determined to be preferable as the amount of bacteria derived from the environment of [plant body associated or symbiotic microbes] is preferably greater among group A. It is more preferable to set it as the form which carries out.

また、［agriculture associated］、［aquatic area 若しくは fluid in state］、［animal associated］、［animal body associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［plant body associated 若しくは symbiotic microbe］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の１０分の１以下であるときに、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, one or more of environment-derived bacteria derived from environments selected from [agriculture associated], [aquatic area or fluid in state], [animal associated], [animal body associated], and [land feature] environments The amount of each of the environment-derived bacteria is selected from the environment of [plant body associated or symbiotic microbe]. It is more preferable to determine that the skin condition based on the parameter of TEWL (transepidermal water loss) is preferable when the value is 1 or less.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＢ群の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚の明るさ（Ｌ^＊）のパラメータに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｂ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［land feature］ The method for diagnosing skin conditions of the present invention uses environment-derived bacteria selected from the following Group B environments defined in the National Institute of Genetics bacteria database (MicrobeDB.jp): , , , or 2 or more, the skin condition based on the skin brightness (L ^* ) parameter is determined to be more favorable.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(Group B)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [land feature]

本発明の実施の形態では、Ｂ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚の明るさ（Ｌ^＊）のパラメータに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, among the environment-derived bacteria selected from the environment-derived bacteria selected from the B group, the more bacteria there are, the brighter the skin, the more preferably 2 or more, more preferably 3 or more, and still more preferably all of the environment-derived bacteria. It is preferable to determine that the skin condition based on the parameter of thickness (L ^* ) is preferable.

また、本発明は、Ｂ群のうち、［agriculture associated］の環境に由来する環境由来菌の菌量が多いほど、皮膚の明るさ（Ｌ^＊）のパラメータに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, in the present invention, the more the amount of environment-derived bacteria derived from the [agriculture associated] environment in Group B, the more preferable the skin condition is determined based on the skin brightness (L ^* ) parameter. It is more preferable that

また、［plant body associated 若しくは symbiotic microbe］、［water associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［agriculture associated］から選ばれる環境に由来する環境由来菌の菌量の１０分の１以下であるときに、皮膚の明るさ（Ｌ^＊）のパラメータに基づく肌状態に基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, among the environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe], [water associated], and [land feature] environments, the amount of each of one or two or more environment-derived bacteria is When the amount of bacteria derived from the environment derived from the environment selected from [agriculture associated] is 1/10 or less, it is determined that the skin condition based on the skin brightness (L ^* ) parameter is preferable. It is more preferable to set it as the form which carries out.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＣ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、肌の弾力性（Ｕｒ／Ｕｆ）のパラメータに基づく肌状態が好ましいと判定する形態とすることが好ましい。
（Ｃ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［park associated］、［land area］ The method for diagnosing skin conditions of the present invention uses 1 of the environment-derived bacteria selected from the following group C defined in the National Institute of Genetics bacteria database (MicrobeDB.jp): Alternatively, it is preferable to determine that the skin condition based on the skin elasticity (Ur/Uf) parameter is more favorable as the amount of two or more environment-derived bacteria increases.
(Group C)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [park associated], [land area]

本発明の実施の形態では、Ｃ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、肌の弾力性（Ｕｒ／Ｕｆ）のパラメータに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, more preferably all environment-derived bacteria selected from group C It is preferable to determine that the skin condition based on the parameter of skin elasticity (Ur/Uf) is more favorable as the amount of bacteria derived from the skin increases.

また、本発明は、Ｃ群のうち、［agriculture associated］の環境に由来する環境由来菌の菌量が多いほど、肌の弾力性（Ｕｒ／Ｕｆ）のパラメータに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, the present invention determines that the skin condition based on the parameters of skin elasticity (Ur/Uf) is more favorable as the amount of environment-derived bacteria derived from the [agriculture associated] environment in Group C increases. It is more preferable to set it as a form.

また、［plant body associated 若しくは symbiotic microbe］、［water associated］、［animal associated］［park associated］、［land area］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［agriculture associated］から選ばれる環境に由来する環境由来菌の菌量の１０分の１以下であるときに、肌の弾力性（Ｕｒ／Ｕｆ）のパラメータに基づく肌状態に基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, one or two or more of the environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe], [water associated], [animal associated], [park associated], and [land area] environments Based on the skin elasticity (Ur/Uf) parameter when the amount of each of the bacteria derived from the environment is 1/10 or less of the amount of bacteria derived from the environment selected from [agriculture associated] It is more preferable to determine that the skin condition is preferable based on the skin condition.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＤ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、シミの少なさに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｄ群）
［plant body associated 若しくは symbiotic microbe］、［water associated］、［animal associated］、［animal body associated］、［land feature］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria selected from the following group D defined in the National Institute of Genetics bacterial database (MicrobeDB.jp): Alternatively, it is preferable to determine that the skin condition based on less spots is more favorable as the amount of two or more environment-derived bacteria increases.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(Group D)
[plant body associated or symbiotic microbe], [water associated], [animal associated], [animal body associated], [land feature]

本発明の実施の形態では、Ｄ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、シミの少なさに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, among the environment-derived bacteria selected from group D, 2 or more, more preferably 3 or more, still more preferably 4 or more, and still more preferably all of the environment-derived bacteria It is preferable to determine that the skin condition based on less spots is more preferable as the number of spots increases.

また、本発明は、Ｄ群のうち、［plant body associated 若しくは symbiotic microbe］及び／又は［land feature］から選ばれる環境に由来する環境由来菌の菌量が多いほど、シミの少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, the present invention provides that the more the amount of bacteria derived from the environment derived from the environment selected from [plant body associated or symbiotic microbe] and / or [land feature] in the D group, the more the skin based on the less spots. It is more preferable to adopt a form in which the state is determined to be favorable.

また、［water associated］、［animal associated］、［animal body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［plant body associated 若しくは symbiotic microbe］及び／又は［land feature］から選ばれる環境に由来する環境由来菌のそれぞれの菌量の１０分の１以下であるときに、シミの少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, among the environment-derived bacteria derived from environments selected from [water associated], [animal associated], and [animal body associated] environments, the amount of each of one or two or more environment-derived bacteria is associated or symbiotic microbe] and/or [land feature]. It is more preferable to set it as the form which carries out.

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］及び／又は［land feature］から選ばれる環境に由来する環境由来菌のうち、全ての環境由来菌の菌量が多いほど、シミの少なさに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, among the environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe] and / or [land feature], the greater the amount of all the environment-derived bacteria, the more stains. It is preferable to determine that the skin condition based on the small amount is preferable.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＥ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、角層水分量の多さに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｅ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria selected from the following group E defined in the National Institute of Genetics bacteria database (MicrobeDB.jp): Alternatively, it is preferable to adopt a form in which the more the amount of two or more environment-derived bacteria is, the more favorable the skin condition based on the amount of water in the stratum corneum is.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(Group E)
[plant body associated or symbiotic microbe], [agriculture associated]

本発明の実施の形態では、Ｅ群から選ばれる環境に由来する環境由来菌のうち、好ましくは全ての環境由来菌の菌量が多いほど、角層水分量の多さに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, among the environment-derived bacteria selected from the environment-derived bacteria selected from group E, the higher the amount of all environment-derived bacteria, the more preferable the skin condition based on the high stratum corneum moisture content. It is preferable to determine that

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される［plant body associated 若しくは symbiotic microbe］の環境に由来する環境由来菌の菌量が多いほど、黄味の少なさに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 The method for diagnosing a skin condition of the present invention uses environment-derived bacteria derived from the [plant body associated or symbiotic microbe] environment defined in the National Institute of Genetics bacteria database (MicrobeDB.jp). It is preferable that the larger the amount, the more favorable the skin condition based on less yellowness.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の好ましい形態によれば、［plant body associated 若しくは symbiotic microbe］の環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、肌の黄味の少なさに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the [plant body associated or symbiotic microbe] environment can be used to non-invasively measure the amount of yellowness of the skin without directly examining the skin. It is possible to diagnose skin conditions based on

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＦ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、頬部の凹凸の少なさに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｆ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［park associated］、［land area］、［land feature］、［fungi body associated］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria selected from the following group F defined in the National Institute of Genetics bacteria database (MicrobeDB.jp): Alternatively, it is preferable to adopt a form in which the skin condition based on less unevenness of the cheek is determined to be more favorable as the amount of two or more environment-derived bacteria increases.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(F group)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [animal body associated], [park associated], [ land area], [land feature], [fungi body associated]

本発明の実施の形態では、Ｆ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは６以上、さらに好ましくは７以上、さらに好ましくは８以上、さらに好ましくは９以上、さらに好ましくは１０以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、頬部の凹凸の少なさに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably 6 or more of the environment-derived bacteria selected from group F, More preferably 7 or more, more preferably 8 or more, more preferably 9 or more, still more preferably 10 or more, more preferably the higher the amount of all bacteria derived from the environment, the skin condition based on the less unevenness of the cheeks. is preferable.

本発明は、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌の菌量が多いほど、頬部の凹凸の少なさに基づく肌状態が好ましいと判定する形態とすることがより好ましい。 The present invention derives from an environment selected from: plant body associated or symbiotic microbe, agriculture associated, water associated, aquatic feature, animal associated, animal body associated, land feature It is more preferable to adopt a form in which the skin condition based on less unevenness of the cheek is determined to be more favorable as the amount of bacteria derived from the environment increases.

また、［aquatic area 若しくは fluid in state］、［park associated］、［land area］、［fungi body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の５分の１以下であるときに、頬部の凹凸の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, one or more of the environment-derived bacteria derived from the environment selected from [aquatic area or fluid in state], [park associated], [land area], and [fungi body associated] environments Each bacterial load is selected from environments of [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [aquatic feature], [animal associated], [animal body associated], [land feature] It is determined that the skin condition based on the small unevenness of the cheek is preferable when the amount of each of the environment-derived bacteria is one-fifth or less of the amount of each of one or more environment-derived bacteria among the environment-derived bacteria. It is more preferable to set it as a form.

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは６以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、頬部の凹凸の少なさに基づく肌状態が好ましいと判定することが好ましい。 In an embodiment of the present invention, selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [aquatic feature], [animal associated], [animal body associated], [land feature] Of the environment-derived bacteria derived from the environment, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably 6 or more, more preferably all of the environment-derived bacteria have a large amount It is preferable to determine that the skin condition based on the degree of unevenness of the cheek is more preferable.

また、本発明は、［animal body associated］の環境に由来する環境由来菌の菌量が多いほど、頬部の凹凸の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 Further, in the present invention, it is more preferable to adopt a form in which the more the amount of environment-derived bacteria derived from the environment of [animal body associated] is, the more preferable the skin condition based on the less unevenness of the cheeks is.

また、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［aquatic feature］、［animal associated］、［park associated］、［land area］、［land feature］、［fungi body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［animal body associated］から選ばれる環境に由来する環境由来菌の菌量の５分の１以下であるときに、頬部の凹凸の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 [plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [park associated], [land area], Of the environment-derived bacteria derived from the environment selected from [land feature] and [fungi body associated], the amount of each of one or more environment-derived bacteria is in the environment selected from [animal body associated] It is more preferable to determine that the skin condition is favorable based on the small unevenness of the cheek when the amount of bacteria derived from the environment is 1/5 or less.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＧ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、目立つ毛穴個数の少なさに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｇ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［park associated］、［land area］、［land feature］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria selected from the following G group defined in the National Institute of Genetics bacterial database (MicrobeDB.jp): Alternatively, it is preferable to adopt a form in which the skin condition based on the smaller number of conspicuous pores is determined to be more preferable as the amount of two or more environment-derived bacteria increases.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(Group G)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [animal body associated], [park associated], [ land area], [land feature]

本発明の実施の形態では、Ｇ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは６以上、さらに好ましくは７以上、さらに好ましくは８以上、さらに好ましくは９以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、目立つ毛穴個数の少なさに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably 6 or more of environment-derived bacteria selected from group G, More preferably 7 or more, more preferably 8 or more, still more preferably 9 or more, more preferably all environment-derived bacteria. preferable.

本発明は、［plant body associated 若しくは symbiotic microbe］、［water associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌の菌量が多いほど、目立つ毛穴個数の少なさに基づく肌状態が好ましいと判定する形態とすることがより好ましい。 In the present invention, the larger the amount of environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe], [water associated], [animal associated], [animal body associated], and [land feature], It is more preferable to determine that the skin condition is preferable based on the small number of conspicuous pores.

また、［agriculture associated］、［aquatic area 若しくは fluid in state］、［aquatic feature］、［park associated］、［land area］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［plant body associated 若しくは symbiotic microbe］、［water associated］、［animal associated］、［animal body associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の３分の１以下であるときに、目立つ毛穴個数の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, one or more of the environment-derived bacteria derived from the environment selected from [agriculture associated], [aquatic area or fluid in state], [aquatic feature], [park associated], and [land area] environments The amount of each of the environment-derived bacteria is derived from an environment selected from [plant body associated or symbiotic microbe], [water associated], [animal associated], [animal body associated], and [land feature] environments. It is more preferable to determine that the skin condition based on the small number of conspicuous pores is preferable when the amount of each of one or more environment-derived bacteria is one third or less of the bacteria. .

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［water associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、目立つ毛穴個数の少なさに基づく肌状態が好ましいと判定することが好ましい。 In an embodiment of the present invention, among environment-derived bacteria derived from an environment selected from [plant body associated or symbiotic microbe], [water associated], [animal associated], [animal body associated], and [land feature], It is preferable to determine that the skin condition based on the small number of conspicuous pores is more favorable as the amount of all environment-derived bacteria is 2 or more, more preferably 3 or more, more preferably 4 or more, and more preferably 4 or more.

また、本発明は、［animal body associated］の環境に由来する環境由来菌の菌量が多いほど、目立つ毛穴個数の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 Further, in the present invention, it is more preferable to determine that the skin condition based on the smaller number of conspicuous pores is more favorable as the amount of environment-derived bacteria derived from the [animal body associated] environment increases.

また、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［aquatic feature］、［animal associated］、［park associated］、［land area］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［animal body associated］の環境に由来する環境由来菌の菌量の１０分の１以下であるときに、目立つ毛穴個数の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 [plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [park associated], [land area], Among the environment-derived bacteria selected from the environment of [land feature], the amount of each of one or more environment-derived bacteria derived from the environment of [animal body associated] It is more preferable to determine that the skin condition based on the small number of conspicuous pores is preferable when the number of pores is one tenth or less.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＨ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、紅斑指数の低さに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｈ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria selected from the following group H defined in the National Institute of Genetics bacteria database (MicrobeDB.jp): Alternatively, it is preferable to adopt a form in which the skin condition based on a low erythema index is determined to be preferable as the amount of two or more environment-derived bacteria increases.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(Group H)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [animal body associated], [land feature]

本発明の好ましい形態によれば、特定の環境由来菌が上記Ｈ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、紅斑指数の低さに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the erythema index can be reduced non-invasively without directly examining the skin, depending on the amount of environment-derived bacteria derived from an environment in which specific environment-derived bacteria are selected from the above group H. It is possible to diagnose skin conditions based on

本発明の実施の形態では、Ｈ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、紅斑指数の低さに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, more preferably all environment-derived bacteria selected from group H It is preferable to determine that the skin condition based on the low erythema index is more preferable as the amount of the bacteria derived from the skin is larger.

本発明は、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal body associated］から選ばれる環境に由来する環境由来菌の菌量が多いほど、紅斑指数の低さに基づく肌状態が好ましいと判定する形態とすることがより好ましい。 In the present invention, the greater the amount of environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], and [animal body associated], the lower the erythema index. It is more preferable to determine that the skin condition based on is preferable.

また、［animal associated］及び／又は［land feature］の環境に由来する環境由来菌のそれぞれの菌量が、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の７分の１以下であるときに、紅斑指数の低さに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, the amount of each of the environment-derived bacteria derived from the [animal associated] and / or [land feature] environment is [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal body skin condition based on a low erythema index when the amount of each of one or two or more of the environmental bacteria derived from the environment selected from the environment of "associated" is 1/7 or less of the amount of each is more preferable.

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal body associated］から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、紅斑指数の低さに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, two or more of environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], and [animal body associated], more preferably is 3 or more, and more preferably, it is determined that the skin condition based on the low erythema index is preferable when the amount of all bacteria derived from the environment is large.

また、本発明は、［animal body associated］の環境に由来する環境由来菌の菌量が多いほど、紅斑指数の低さに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 Further, in the present invention, it is more preferable to determine that the skin condition based on the low erythema index is more favorable as the amount of environment-derived bacteria derived from the [animal body associated] environment increases.

また、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［animal body associated］の環境に由来する環境由来菌の菌量の８分の１以下であるときに、紅斑指数の低さに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, one or more of the environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], and [land feature] environments When the amount of each environment-derived bacteria is 1/8 or less of the amount of environment-derived bacteria derived from the [animal body associated] environment, it is determined that the skin condition based on the low erythema index is preferable. It is more preferable to set it as the form which carries out.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＩ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、キメ状態の良さに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｉ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［land area］、［land feature］、［fungi body associated］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria selected from the following group I as defined in the National Institute of Genetics bacteria database (MicrobeDB.jp): Alternatively, it is preferable to adopt a form in which the more the amount of two or more environment-derived bacteria is, the more favorable the skin condition based on the good texture condition is.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(Group I)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [land area], [land feature], [fungi body associated]

本発明の実施の形態では、Ｉ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは６以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、キメ状態の良さに基づく肌状態が好ましいと判定することが好ましい。 In an embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably 6 or more among environment-derived bacteria selected from Group I, More preferably, the larger the amount of bacteria derived from the environment, the more preferable the skin condition is determined based on the good texture condition.

本発明は、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［land area］、［land feature］、［fungi body associated］から選ばれる環境に由来する環境由来菌の菌量が多いほど、キメ状態の良さに基づく肌状態が好ましいと判定する形態とすることがより好ましい。 The present invention provides an environment-derived fungus derived from an environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [land area], [land feature], and [fungi body associated]. It is more preferable to determine that the skin condition based on the good texture condition is more preferable as the amount is larger.

また、［animal associated］から選ばれる環境に由来する環境由来菌の菌量が、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［land area］、［land feature］、［fungi body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の５分の１以下であるときに、キメ状態の良さに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, the amount of environment-derived bacteria derived from the environment selected from [animal associated] is [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [land area], [land feature], Based on good texture when the amount of each of the environment-derived bacteria selected from the [fungi body associated] environment is one-fifth or less of the amount of each of one or more environment-derived bacteria It is more preferable to use a form in which it is determined that the skin condition is favorable.

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［land area］、［land feature］、［fungi body associated］から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、キメ状態の良さに基づく肌状態が好ましいと判定することが好ましい。 In an embodiment of the present invention, an environment derived from an environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [land area], [land feature], [fungi body associated] It is possible to determine that the skin condition based on the good texture condition is more preferable when the amount of bacteria derived from the environment is 2 or more, more preferably 3 or more, more preferably 4 or more, and more preferably all of the bacteria derived from the environment. preferable.

また、本発明は、［water associated］の環境に由来する環境由来菌の菌量が多いほど、キメ状態の良さに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 Further, in the present invention, it is more preferable to determine that the skin condition based on the good texture condition is more favorable as the amount of environment-derived bacteria derived from the [water associated] environment increases.

また、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［animal associated］、［land area］、［land feature］、［fungi body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［water associated］の環境に由来する環境由来菌の菌量の３分の１以下であるときに、キメ状態の良さに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, among environment-derived bacteria derived from environments selected from [plant body associated or symbiotic microbe], [agriculture associated], [animal associated], [land area], [land feature], and [fungi body associated] environments , Skin condition based on good skin texture when the amount of each of one or more environment-derived bacteria is one-third or less of the amount of environment-derived bacteria derived from the [water associated] environment is more preferable.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＪ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮脂量の少なさに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｊ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［animal associated］、［animal body associated］、［land area］、［land feature］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria selected from the following group J defined in the National Institute of Genetics bacteria database (MicrobeDB.jp): Alternatively, it is preferable to adopt a form in which the more the amount of two or more environment-derived bacteria is, the more favorable the skin condition based on the smaller amount of sebum is.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(Group J)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [animal associated], [animal body associated], [land area], [land feature]

本発明の実施の形態では、Ｊ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは６以上、さらに好ましくは７以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮脂量の少なさに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably 6 or more of environment-derived bacteria selected from group J, It is more preferable to judge that the skin condition based on the small amount of sebum is more favorable as the amount of all environment-derived bacteria is more preferably 7 or more, more preferably more.

本発明は、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌の菌量が多いほど、皮脂量の少なさに基づく肌状態が好ましいと判定する形態とすることがより好ましい。 The present invention provides an environment-derived fungus derived from an environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [animal body associated], and [land feature]. It is more preferable to determine that the skin condition based on the smaller amount of sebum is more preferable as the amount of sebum increases.

また、［aquatic area 若しくは fluid in state］及び／又は［land area］の環境に由来する環境由来菌のそれぞれの菌量が、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の３分の１以下であるときに、皮脂量の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, the amount of each of the environment-derived bacteria derived from the environment of [aquatic area or fluid in state] and / or [land area] is [plant body associated or symbiotic microbe], [agriculture associated], [water associated] , [animal associated], [animal body associated], and [land feature] environment-derived bacteria, one or two or more of the environment-derived bacteria, each of which is one-third or less of the amount of each It is further preferable to determine that the skin condition based on the small amount of sebum is preferable when .

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮脂量の少なさに基づく肌状態が好ましいと判定することが好ましい。 In an embodiment of the invention, an environment derived from an environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [animal body associated], [land feature] Of the bacteria derived from the environment, 2 or more, more preferably 3 or more, more preferably 4 or more, still more preferably 5 or more, more preferably all environment-derived bacteria, the greater the amount of bacteria, the lower the skin condition based on the amount of sebum. is preferable.

また、本発明は、［animal body associated］の環境に由来する環境由来菌の菌量が多いほど、皮脂量の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 Further, in the present invention, it is more preferable to adopt a form in which the more the amount of environment-derived bacteria derived from the [animal body associated] environment is, the more favorable the skin condition based on the smaller amount of sebum is.

また、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［animal associated］、［land area］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［animal body associated］から選ばれる環境に由来する環境由来菌の菌量の３分の１以下であるときに、皮脂量の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, an environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [animal associated], [land area], and [land feature] Among the environment-derived bacteria derived from , the amount of each of 1 or 2 or more environment-derived bacteria derived from the environment selected from [animal body associated] is 1/3 or less of the amount of environment-derived bacteria derived from the environment. Sometimes, it is more preferable to determine that the skin condition based on the small amount of sebum is preferable.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＫ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、シワ本数の少なさに基づく肌状態が好ましいと判定する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｋ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［animal associated］、［animal body associated］、［land feature］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria selected from the following K group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp): Alternatively, it is preferable to adopt a form in which the skin condition based on the smaller number of wrinkles is determined to be more favorable as the amount of two or more environment-derived bacteria increases.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(K group)
[plant body associated or symbiotic microbe], [agriculture associated], [animal associated], [animal body associated], [land feature]

本発明の実施の形態では、Ｋ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、シワ本数の少なさに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, among the environment-derived bacteria selected from the K group, 2 or more, more preferably 3 or more, still more preferably 4 or more, and still more preferably all of the environment-derived bacteria It is preferable to determine that the skin condition based on the smaller number of wrinkles is more preferable as the number of wrinkles increases.

本発明は、［agriculture associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌の菌量が多いほど、シワ本数の少なさに基づく肌状態が好ましいと判定する形態とすることがより好ましい。 The present invention provides a skin condition based on a smaller number of wrinkles as the amount of environment-derived bacteria derived from an environment selected from [agriculture associated], [animal associated], [animal body associated], and [land feature] increases. is more preferable.

また、［plant body associated 若しくは symbiotic microbe］の環境に由来する環境由来菌の菌量が、［agriculture associated］、［animal associated］、［animal body associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の１０分の１以下であるときに、シワ本数の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, the amount of environment-derived bacteria derived from the [plant body associated or symbiotic microbe] environment is selected from [agriculture associated], [animal associated], [animal body associated], and [land feature] environments. When the amount of each of one or more of the environment-derived bacteria is 1/10 or less of the amount of each of the environment-derived bacteria, the skin condition based on the small number of wrinkles is determined to be preferable. is more preferred.

本発明の実施の形態では、［agriculture associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、シワ本数の少なさに基づく肌状態が好ましいと判定することが好ましい。 In an embodiment of the present invention, two or more, more preferably three or more, environment-derived bacteria derived from an environment selected from [agriculture associated], [animal associated], [animal body associated], and [land feature], More preferably, it is determined that the skin condition based on the smaller number of wrinkles is more favorable as the amount of all environment-derived bacteria increases.

また、本発明は、［agriculture associated］及び／又は［land feature］から選ばれる環境に由来する環境由来菌の菌量が多いほど、シワ本数の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, in the present invention, the more the amount of bacteria derived from the environment selected from [agriculture associated] and/or [land feature], the more preferable the skin condition based on the smaller number of wrinkles. more preferably.

また、［plant body associated 若しくは symbiotic microbe］、［animal associated］、［animal body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［agriculture associated］及び／又は［land feature］から選ばれる環境に由来する環境由来菌のそれぞれの菌量の２分の１以下であるときに、シワ本数の少なさに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, among the environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe], [animal associated], and [animal body associated] environments, the amount of each of one or two or more environment-derived bacteria , [agriculture associated] and/or [land feature]. It is more preferable to set it as the form of determination.

本発明の実施の形態では、［agriculture associated］及び／又は［land feature］から選ばれる環境に由来する環境由来菌のうち、全ての環境由来菌の菌量が多いほど、シワ本数の少なさに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, among the environment-derived bacteria derived from the environment selected from [agriculture associated] and / or [land feature], the larger the amount of all the environment-derived bacteria, the less wrinkles. It is preferable to determine that the skin condition based on is preferable.

本発明の肌状態の診断方法は、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＬ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、メラニン指数の低さに基づく肌状態が好ましいと判定するする形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。
（Ｌ群）
［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］ The method for diagnosing skin conditions of the present invention uses one of the environment-derived bacteria derived from the following group L selected from the bacteria database (MicrobeDB.jp) of the National Institute of Genetics (National Institute of Genetics). Alternatively, it is preferable to adopt a form in which the skin condition based on a low melanin index is determined to be preferable as the amount of two or more environment-derived bacteria increases.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.
(L group)
[agriculture associated], [water associated], [animal associated], [animal body associated], [land feature]

本発明の好ましい形態によれば、特定の環境由来菌が上記Ｌ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、メラニン指数の低さに基づく肌状態を診断することができる。 According to a preferred embodiment of the present invention, the melanin index can be reduced non-invasively without directly examining the skin, depending on the amount of environment-derived bacteria derived from an environment in which specific environment-derived bacteria are selected from the above group L. It is possible to diagnose skin conditions based on

本発明の実施の形態では、Ｌ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、メラニン指数の低さに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, of the environment-derived bacteria selected from the environment selected from the L group, 2 or more, more preferably 3 or more, still more preferably 4 or more, and still more preferably all of the environment-derived bacteria It is preferable to determine that the skin condition based on the low melanin index is preferable as the number increases.

本発明は、［agriculture associated］、［animal associated］、［animal body associated］、の環境に由来する環境由来菌の菌量が多いほど、メラニン指数の低さに基づく肌状態が好ましいと判定する形態とすることがより好ましい。 The present invention is a mode in which the skin condition based on the low melanin index is determined to be preferable as the amount of bacteria derived from the environment of [agriculture associated], [animal associated], and [animal body associated] increases. is more preferable.

また、［water associated］、［land feature］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量が、［agriculture associated］、［animal associated］、［animal body associated］の環境から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌のそれぞれの菌量の２分の１以下であるときに、メラニン指数の低さに基づく肌状態が好ましいと判定する形態とすることがさらに好ましい。 In addition, among the environment-derived bacteria derived from environments selected from [water associated] and [land feature] environments, the amount of each of one or more environment-derived bacteria is [agriculture associated], [animal associated] A low melanin index when the amount of each of one or two or more of the environment-derived bacteria derived from the environment selected from the environment of [animal body associated] is 1/2 or less. It is further preferable to determine that the skin condition based on is preferable.

本発明の実施の形態では、［agriculture associated］、［animal associated］、［animal body associated］から選ばれる環境に由来する環境由来菌のうち、２以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、メラニン指数の低さに基づく肌状態が好ましいと判定することが好ましい。 In the embodiment of the present invention, two or more of the environment-derived bacteria derived from the environment selected from [agriculture associated], [animal associated], and [animal body associated], and more preferably all of the environment-derived bacteria It is preferable to determine that the skin condition based on the low melanin index is more preferable as the number of .

＜２＞皮膚常在細菌叢の多様性の推測方法
本発明は、特定の肌状態と相関のある特定の環境由来菌の菌量に基づく、皮膚常在細菌叢の多様性の推測方法にも関する。 <2> Method for estimating the diversity of the skin flora The present invention also provides a method for estimating the diversity of the skin flora based on the amount of specific environment-derived bacteria that are correlated with a specific skin condition. related.

本発明の皮膚常在細菌叢の多様性の推測方法は、
皮膚常在細菌叢に存在する菌の菌種を同定する菌種同定工程と、
同定した菌種と、環境由来菌情報と、に基づき前記皮膚常在細菌叢における特定の肌状態と相関のある特定の環境由来菌の菌量を計測する計測工程と、
計測結果に基づき、前記特定の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると判定する判定工程と、を有し、
前記環境由来菌情報は、菌種又は菌属に関する情報と、その菌種又は菌属の検出された環境に関する情報と、を紐づけた情報であり、
前記特定の肌状態は、前記多様性と相関のある肌状態であることを特徴とする。 The method for estimating the diversity of skin flora of the present invention comprises:
a bacterial species identification step of identifying bacterial species present in the skin flora;
a measurement step of measuring the amount of specific environment-derived bacteria in the indigenous skin flora that correlates with a specific skin condition based on the identified bacterial species and information on the environment-derived bacteria;
a determination step of determining, based on the measurement results, that the greater the amount of the specific environment-derived bacteria, the more diverse the indigenous skin microflora;
The environment-derived bacterium information is information that links information on the bacterium species or genus with information on the environment in which the bacterium species or genus is detected,
The specific skin condition is characterized by being a skin condition correlated with the diversity.

ここで、上述の診断方法に関する技術的事項のうち、皮膚常在細菌叢の多様性の推測方法に妥当する技術的事項については、前述した＜１＞肌状態の診断方法における説明を準用することができる。 Here, among the technical matters related to the diagnostic method described above, the technical matters applicable to the method for estimating the diversity of the indigenous skin flora shall be applied mutatis mutandis to the above-mentioned <1> Diagnosis method for skin condition. can be done.

以下、本発明の皮膚常在細菌叢の多様性の推測方法における、好ましい実施の形態について詳述する。 Hereinafter, preferred embodiments of the method for estimating the diversity of skin flora of the present invention will be described in detail.

本発明の実施の形態では、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＧ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 In an embodiment of the present invention, one or two of the environment-derived bacteria derived from the environment selected from the above G group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の好ましい実施の形態によれば、上記のＧ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮膚常在細菌叢の多様性を推測することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above G group can be used to non-invasively diversify the indigenous skin flora without directly examining the skin. gender can be inferred.

本発明の実施の形態では、上記のＧ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは６以上、さらに好ましくは７以上、さらに好ましくは８以上、さらに好ましくは９以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably 6 of the environment-derived bacteria selected from the above group G Above, more preferably 7 or more, more preferably 8 or more, still more preferably 9 or more, and more preferably all environment-derived bacteria. It is preferable to set it as a form.

本発明は、［plant body associated 若しくは symbiotic microbe］、［water associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 The present invention provides one or more of environment-derived bacteria derived from an environment selected from [plant body associated or symbiotic microbe], [water associated], [animal associated], [animal body associated], and [land feature] It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.

また、本発明は、［animal body associated］の環境に由来する環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 Further, in the present invention, it is preferable to adopt a form in which the greater the amount of environment-derived bacteria derived from the [animal body associated] environment, the more diverse the indigenous skin microflora is estimated.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の実施の形態では、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＪ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 In the embodiment of the present invention, one or two of the environment-derived bacteria derived from the environment selected from the above J group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の好ましい実施の形態によれば、上記のＪ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮膚常在細菌叢の多様性を推測することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above J group can be used to non-invasively diversify the indigenous skin flora without directly examining the skin. gender can be inferred.

本発明の実施の形態では、上記のＪ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは６以上、さらに好ましくは７以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably 6 of the environment-derived bacteria selected from the above group J As described above, more preferably 7 or more, and more preferably all of the environment-derived bacteria, it is preferable to assume that the diversity of the indigenous skin flora is high.

本発明は、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 Among environment-derived bacteria derived from an environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [animal body associated], and [land feature] , 1 or 2 or more of the environment-derived bacteria, it is preferable to adopt a form in which it is assumed that the diversity of the indigenous skin flora is high.

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In an embodiment of the invention, an environment derived from an environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [animal body associated], [land feature] Among the bacteria derived from the skin, 2 or more, more preferably 3 or more, more preferably 4 or more, more preferably 5 or more, and more preferably all of the environment-derived bacteria. It is preferable to take the form of inferring wealth.

また、本発明は、［animal body associated］の環境に由来する環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 Further, in the present invention, it is preferable to adopt a form in which the greater the amount of environment-derived bacteria derived from the [animal body associated] environment, the more diverse the indigenous skin microflora is estimated.

本発明の実施の形態では、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＨ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 In an embodiment of the present invention, one or two of the environment-derived bacteria derived from the environment selected from the above group H defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の好ましい実施の形態によれば、上記のＨ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮膚常在細菌叢の多様性を推測することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above H group can be used to non-invasively diversify the indigenous skin flora without directly examining the skin. gender can be inferred.

本発明の実施の形態では、上記のＨ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably all of the environment-derived bacteria selected from the above group H It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.

本発明は、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal body associated］から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 The present invention provides one or more environment-derived bacteria selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], and [animal body associated]. It is preferable to adopt a form in which the greater the amount of bacteria, the more diverse the indigenous skin microflora is assumed to be.

本発明の実施の形態では、［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal body associated］から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the embodiment of the present invention, two or more of environment-derived bacteria derived from the environment selected from [plant body associated or symbiotic microbe], [agriculture associated], [water associated], and [animal body associated], more preferably is 3 or more, and more preferably, it is assumed that the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.

本発明の実施の形態では、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＡ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 In an embodiment of the present invention, one or two of the environment-derived bacteria derived from the environment selected from the above group A defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の好ましい実施の形態によれば、上記のＡ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮膚常在細菌叢の多様性を推測することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above group A can be used to non-invasively diversify the indigenous skin flora without directly examining the skin. gender can be inferred.

本発明の実施の形態では、上記のＡ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably all of the environment-derived bacteria selected from the above group A It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.

本発明は、Ａ群のうち、好ましくは［plant body associated 若しくは symbiotic microbe］の環境に由来する環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the present invention, in Group A, preferably, the higher the amount of environment-derived bacteria derived from the [plant body associated or symbiotic microbe] environment, the more diverse the indigenous skin flora. It is preferable to

本発明の実施の形態では、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＬ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 In an embodiment of the present invention, one or two of the environment-derived bacteria derived from the environment selected from the above group L defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の好ましい実施の形態によれば、上記のＬ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮膚常在細菌叢の多様性を推測することができる。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above L group can be used to non-invasively diversify the indigenous skin flora without directly examining the skin. gender can be inferred.

本発明の実施の形態では、上記のＬ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは４以上、さらに好ましくは５以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the embodiment of the present invention, 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, still more preferably all of the environment-derived bacteria selected from the above group L It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.

本発明は、［agriculture associated］、［animal associated］、［animal body associated］、の環境に由来する環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 The present invention presumes that the greater the amount of environment-derived bacteria derived from the [agriculture associated], [animal associated], and [animal body associated] environments, the more diverse the indigenous skin flora. It is preferable to set it as a form.

本発明の実施の形態では、［agriculture associated］、［animal associated］、［animal body associated］から選ばれる環境に由来する環境由来菌のうち、２以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the embodiment of the present invention, two or more of the environment-derived bacteria derived from the environment selected from [agriculture associated], [animal associated], and [animal body associated], and more preferably all of the environment-derived bacteria It is preferable to adopt a form in which the greater the number of , the more diverse the indigenous skin microflora is assumed to be.

本発明の実施の形態では、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＢ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 In an embodiment of the present invention, one or two of the environment-derived bacteria derived from the environment selected from the above B group defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) It is preferable to adopt a form in which the greater the amount of bacteria derived from the environment, the more diverse the indigenous skin flora.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の好ましい実施の形態によれば、上記のＢ群から選ばれる環境に由来する環境由来菌の菌量によって、直接肌を検査することなく、非侵襲的に、皮膚常在細菌叢の多様性を推測することができる。
ここで、菌又は該菌の属する菌属が検出された環境のうち、菌又は該菌の属する菌属の平均存在比率の最も高い環境を、該菌が由来する環境であるとする。 According to a preferred embodiment of the present invention, the amount of environment-derived bacteria derived from the environment selected from the above B group can be used to non-invasively diversify the indigenous skin flora without directly examining the skin. gender can be inferred.
Here, of the environments in which the bacterium or the genus to which the bacterium belongs are detected, the environment in which the average abundance ratio of the bacterium or the genus to which the bacterium belongs is the highest is defined as the environment from which the bacterium originates.

本発明の実施の形態では、上記のＢ群から選ばれる環境に由来する環境由来菌のうち、２以上、より好ましくは３以上、さらに好ましくは全ての環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In the embodiment of the present invention, 2 or more of the environment-derived bacteria selected from the above group B, more preferably 3 or more, and still more preferably all the environment-derived bacteria, the more the amount of bacteria, the more the skin It is preferable to adopt a form that is presumed to be rich in diversity of indigenous flora.

また、本発明は、Ｂ群のうち、［agriculture associated］の環境に由来する環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると推測する形態とすることが好ましい。 In addition, in the present invention, it can be assumed that the greater the amount of environment-derived bacteria derived from the [agriculture associated] environment in Group B, the more diverse the indigenous skin flora. preferable.

＜３＞特定の環境由来菌の菌量の推測方法
本発明は、特定の肌状態と相関のある特定の環境由来菌の菌量の推測方法にも関する。 <3> Method for estimating the amount of specific environment-derived bacteria The present invention also relates to a method for estimating the amount of specific environment-derived bacteria correlated with a specific skin condition.

本発明の特定の環境由来菌の菌量の推測方法は、皮膚常在細菌叢における多様性を解析する多様性解析工程と、
解析結果に基づき、前記多様性に富んでいるほど、前記皮膚常在細菌叢における特定の肌状態と相関のある特定の環境由来菌の菌量が多いと判定する判定工程と、
を有し、
前記特定の肌状態は、前記多様性と相関のある肌状態であることを特徴とする。 The method for estimating the amount of specific environment-derived bacteria of the present invention comprises a diversity analysis step of analyzing diversity in the skin flora,
a determination step of determining, based on the analysis results, that the greater the diversity, the greater the amount of specific environment-derived bacteria correlated with a specific skin condition in the indigenous skin flora;
has
The specific skin condition is characterized by being a skin condition correlated with the diversity.

ここで、多様性解析工程は、前述した菌種同定工程と同様の方法により行うことができる。 Here, the diversity analysis step can be performed by the same method as the bacterial species identification step described above.

なお、上述の診断方法に関する技術的事項のうち、特定の環境由来菌の菌量の推測方法に妥当する技術的事項については、前述した＜２＞皮膚常在細菌叢の多様性の推測方法における説明を準用することができる。 Among the technical matters related to the diagnostic method described above, the technical matters applicable to the method for estimating the amount of bacteria derived from a specific environment are described in <2> Method for estimating diversity of indigenous skin flora. The explanation can be applied mutatis mutandis.

＜４＞特定の肌状態と相関のある菌の属性を解析する方法
本発明は、特定の肌状態と相関のある菌の属性を解析する方法にも関する。 <4> Method for analyzing attributes of bacteria correlated with specific skin conditions The present invention also relates to methods for analyzing attributes of bacteria correlated with specific skin conditions.

本発明の特定の肌状態と相関のある菌の属性を解析する方法は、
前記特定の肌状態の良い被験者群と悪い被験者群の皮膚常在細菌叢に存在する菌において、両群間で菌量に差のある菌種又は菌属を特定する特定工程と、
前記特定工程で特定された菌種又は菌属の属性に関する情報を取得することを特徴とする。 The method of analyzing the attributes of bacteria correlated with a specific skin condition of the present invention includes:
an identifying step of identifying the species or genus of bacteria present in the indigenous skin flora of the specific group of subjects with good skin condition and the group of subjects with poor skin condition, which have a difference in the amount of bacteria between the two groups;
It is characterized by acquiring information on the attributes of the bacterial species or genus identified in the identifying step.

本発明によれば、皮膚常在細菌叢における多様性によって、直接肌を検査することなく、非侵襲的に、特定の肌状態と相関のある特定の環境由来菌の菌量を推測することができる。
以下、本発明の、特定の肌状態と相関のある菌の属性を解析する方法の実施形態について説明するが、本発明の技術的範囲は以下の実施形態に限定されないことは言うまでもない。 According to the present invention, it is possible to noninvasively estimate the amount of specific environment-derived bacteria that are correlated with a specific skin condition, without directly examining the skin, based on the diversity of the indigenous skin flora. can.
Hereinafter, embodiments of the method of analyzing the attributes of bacteria that are correlated with specific skin conditions will be described, but the technical scope of the present invention is not limited to the following embodiments.

本発明の特定の肌状態と相関のある菌の属性を解析する方法における、被験者は、アトピーなどの疾患を有しない被験者であることが望ましい。 In the method of analyzing the attributes of bacteria correlated with a specific skin condition of the present invention, the subject is desirably a subject who does not have a disease such as atopy.

特定の肌状態の良い被験者群と悪い被験者群のグループ分けは、取得した被験者の肌状態に関するパラメータに基づき、測定値が下位１０％であった被験者群と、測定値が上位１０％であった被験者群とに、グループ分けを行う形態とすることが望ましい。なお、特定の肌状態の良い被験者群と悪い被験者群とにグループ分けをすることができれば、その方法、基準に特に制限はない。 The grouping of subjects with good and bad skin conditions was based on the obtained parameters related to the skin condition of the subjects, and the subjects with the bottom 10% of the measured values and the subjects with the top 10% of the measured values. It is desirable to divide the subjects into groups. There is no particular limitation on the method and criteria as long as the group can be grouped into a group of subjects with good skin condition and a group of subjects with bad skin condition.

ここで、被験者の皮膚常在細菌叢における、菌種又は菌属を特定（同定）する方法についての好ましい形態は前述した通りである。 Here, the preferred form of the method for specifying (identifying) the species or genus in the normal skin flora of the subject is as described above.

本発明の特定の肌状態と相関のある菌の属性を解析する方法の好ましい実施の形態では、解析する属性が菌の由来環境であることが望ましい。 In a preferred embodiment of the method of analyzing the attribute of bacteria correlated with a specific skin condition of the present invention, the attribute to be analyzed is desirably the origin environment of the bacteria.

なお、上述の診断方法に関する技術的事項のうち、特定の肌状態と相関のある菌の属性を解析する方法に妥当する技術的事項については、前述した＜１＞肌状態の診断方法、前述した＜２＞皮膚常在細菌叢の多様性の推測方法における説明を準用することができる。 Among the technical matters related to the diagnostic method described above, the technical matters applicable to the method of analyzing the attributes of bacteria correlated with a specific skin condition are the above-mentioned <1> skin condition diagnosis method, the above-mentioned <2> The description in the method for estimating diversity of indigenous skin flora can be applied mutatis mutandis.

＜５＞肌状態改善作用を有する物質又は美容方法のスクリーニング方法
本発明は、肌状態と相関性のある特定の肌状態と相関のある特定の環境由来菌の菌量に基づく、肌状態改善作用を有する物質又は美容方法のスクリーニング方法にも関する。 <5> Screening method for substances or beauty methods having skin condition-improving effects The present invention provides skin condition-improving effects based on the amount of specific environment-derived bacteria that are correlated with specific skin conditions that are correlated with skin conditions. It also relates to a screening method for substances or cosmetic methods having

本発明によれば、特定の肌状態と相関のある特定の環境由来菌の菌量に基づき、肌状態を改善する物質又は美容方法をスクリーニングすることができる。
以下、本発明のスクリーニング方法の実施形態について説明するが、本発明の技術的範囲は以下の実施形態に限定されないことは言うまでもない。 According to the present invention, it is possible to screen substances or cosmetic methods that improve skin conditions based on the amount of specific environment-derived bacteria that are correlated with specific skin conditions.
Embodiments of the screening method of the present invention will be described below, but it goes without saying that the technical scope of the present invention is not limited to the following embodiments.

本発明のスクリーニング方法におけるスクリーニング対象としては、特に限定されず、たとえば、市販の化合物（ペプチドを含む）、公知化合物（ペプチドを含む）、コンビナトリアル・ケミストリー技術によって得られた化合物群、植物や海洋生物由来の天然成分、動物組織抽出物などの物質を挙げることができる。
また、顔面パックやマッサージなどの美容方法も本発明のスクリーニング方法のスクリーニング対象とすることができる。 The screening target in the screening method of the present invention is not particularly limited, and examples include commercially available compounds (including peptides), known compounds (including peptides), compounds obtained by combinatorial chemistry techniques, plants and marine organisms. Materials such as natural ingredients, animal tissue extracts and the like can be mentioned.
In addition, cosmetic methods such as face packs and massages can also be screened by the screening method of the present invention.

本発明は以下のような工程を有する実施の形態とすることが好ましい。すなわち、スクリーニング対象の物質又は美容方法を被験者に適用する適用工程と、適用工程前後の皮膚常在細菌叢に存在する菌の菌種を同定する菌種同定工程と、
同定した菌種と、環境由来菌情報と、に基づき皮膚常在細菌叢における特定の肌状態と相関のある特定の環境由来菌の菌量を計測する計測工程と、
特定の肌状態と相関のある特定の環境由来菌の菌量が増えるほど、肌状態の改善作用に優れると判定する判定工程と、を有する実施の形態とすることが好ましい。 It is preferable that the present invention be an embodiment having the following steps. That is, an application step of applying a screening target substance or cosmetic method to a subject, a bacterial species identification step of identifying the bacterial species present in the skin flora before and after the application step,
a measurement step of measuring the amount of specific environment-derived bacteria in the indigenous skin flora that correlates with a specific skin condition based on the identified bacterial species and the information on the environment-derived bacteria;
It is preferable to provide an embodiment having a determination step of determining that the greater the amount of specific environment-derived bacteria correlated with a specific skin condition, the more excellent the effect of improving the skin condition.

適用工程における、物質又は美容方法の適用態様、適用期間、適用頻度については特に制限はない。なお、スクリーニング対象が物質である場合には、たとえば、液剤、粉末剤、顆粒剤、錠剤などの経口剤の形態として被験者に経口投与してもよいし、注射剤、外用液剤、軟膏等の皮膚外用剤等の形態として非経口的に投与してもよい。 There are no particular restrictions on the mode of application, period of application, and frequency of application of the substance or cosmetic method in the application step. When the screening target is a substance, for example, it may be administered orally to the subject in the form of oral preparations such as liquids, powders, granules, and tablets. It may be administered parenterally in the form of an external preparation or the like.

なお、上述の診断方法に関する技術的事項のうち、特定の肌状態と相関のある菌の属性を解析する方法に妥当する技術的事項については、前述した＜１＞肌状態の診断方法における説明を準用することができる。 Among the technical matters related to the diagnostic method described above, the technical matters applicable to the method of analyzing the attributes of bacteria that are correlated with a specific skin condition are explained in the above-mentioned <1> Diagnosis method of skin condition. Applicable mutatis mutandis.

以下、本発明の基礎となる知見を裏付ける各種試験結果を示す。 Various test results supporting the findings underlying the present invention are shown below.

＜試験１＞各環境由来菌の菌量と、各肌状態との関連
以下の肌状態に関するパラメータと皮膚表面の細菌叢との関連性を調べた。なお、以下、に記載の菌量は、特に断りのない限り、各細菌のリード数でありその細菌種の存在量を表す。 <Test 1> Relation between the amount of each environment-derived bacteria and each skin condition The relation between the following skin condition-related parameters and the bacterial flora on the skin surface was investigated. Unless otherwise specified, the amount of bacteria described below is the number of reads for each bacterium and represents the abundance of that bacterium species.

（１）被験者の肌状態の測定
被験者となる女性は３０代のアトピーなどの疾患を有しない健常日本人女性２９３名である。
まず、被験者の肌状態に関するパラメータの取得を行った。取得したパラメータは、ＴＥＷＬ（経皮水分蒸散量）、皮膚の明るさ（Ｌ^＊）、肌の弾力性（Ｕｒ／Ｕｆ）、シミの少なさ、角層水分量の多さ、肌の黄味の少なさ、頬部の凹凸の少なさ、目立つ毛穴個数の少なさ、紅斑指数の低さ、キメ状態の良さ、皮脂量の少なさ、シワ本数の少なさ、メラニン指数の低さ、である。 (1) Measurement of Skin Conditions of Test Subjects The women to be tested are 293 healthy Japanese women in their 30s who do not have diseases such as atopy.
First, parameters related to the subject's skin condition were acquired. The acquired parameters are TEWL (transepidermal water loss), skin brightness (L ^* ), skin elasticity (Ur/Uf), few spots, large stratum corneum moisture content, and yellowness of skin. little unevenness on the cheeks, few conspicuous pores, low erythema index, good texture, little sebum, few wrinkles, and low melanin index. .

被験者のＴＥＷＬ（経皮水分蒸散量）に関するパラメータは、ＴＥＷＬ（CK electronic GmbH社製、TEWAMETER TM300）を用いることにより得た。 Parameters related to TEWL (transepidermal water loss) of subjects were obtained by using TEWL (TEWAMETER TM300, manufactured by CK electronic GmbH).

被験者の皮膚の明るさに関するパラメータは、分光測色計（コニカミノルタセンシング（株）製、ＣＭ２６００ｄ）を用いて、光源Ｄ－６５、視野１０度、SCE法にてＬ^＊ａ^＊ｂ^＊表色系により、Ｌ^＊値を測定し、評価することにより得た。 The parameters related to the brightness of the subject's skin were measured using a spectrophotometer (CM2600d, manufactured by Konica Minolta Sensing Co., Ltd.) with a light source of D-65, a field of view of 10 degrees, and L ^* a ^* b ^* colorimetry by the SCE method. It was obtained by measuring and evaluating the L ^* value according to the system.

被験者の皮膚の弾力性に関するパラメータは、皮膚粘弾性測定装置（CK electronic GmbH社製ＣｕｔｏｍｅｔｅｒＭＰＡ５８０）を用いて皮膚を、吸引圧４００ｍｂ、吸引時間２秒、開放時間２秒を１サイクルとする測定を１回行い、得られた「Ｒ０」～「Ｒ８」の結果のうちＲ７（戻り率：Ｕｒ／Ｕｆ）の結果から得た。 The parameters related to the skin elasticity of the subject were measured using a skin viscoelasticity measuring device (CK electronic GmbH Cutometer MPA580), with a suction pressure of 400 mb, a suction time of 2 seconds, and an open time of 2 seconds as one cycle. It was obtained from the result of R7 (return rate: Ur/Uf) among the results of "R0" to "R8" obtained by performing once.

被験者のシミの少なさに関するパラメータは、皮膚画像解析カウンセリングシステム（Canfield社製、ＶＩＳＩＡ－ＥＶＯＬＵＴＩＯＮ）を用いて皮膚画像を採取し、画像解析することにより得た。 Parameters relating to the degree of blemishes of subjects were obtained by taking skin images using a skin image analysis counseling system (VISIA-EVOLUTION manufactured by Canfield) and analyzing the images.

被験者の角層水分量の多さに関するパラメータは、I.B.S社製のＳｋｉｃｏｎ－２００ＥＸを用いて、頬を5回ずつ測定して、平均値を測定値として評価することにより得た。 A parameter relating to the amount of water in the stratum corneum of the subject was obtained by measuring the cheeks five times using Skicon-200EX manufactured by I.B.S and evaluating the average value as the measured value.

被験者の肌の黄味の少なさに関するパラメータは、分光測色計（コニカミノルタセンシング（株）製、ＣＭ２６００ｄ）を用いて、光源Ｄ－６５、視野１０度、SCE法にてＬ^＊ａ^＊ｂ^＊表色系により、ｂ^＊値を測定し、評価することにより得た。 The parameters related to the degree of yellowness of the subject's skin were measured using a spectrophotometer (CM2600d, manufactured by Konica Minolta Sensing Co., Ltd.) with a light source of D-65, a field of view of 10 degrees, and L ^* a ^* b by the SCE method. ^* Obtained by measuring and evaluating the b ^* value using a color system.

被験者の頬部の凹凸の少なさに関するパラメータは、皮膚画像解析カウンセリングシステム（Canfield社製、ＶＩＳＩＡ－ＥＶＯＬＵＴＩＯＮ）を用いて皮膚画像を採取し、画像解析することにより得た。 A parameter relating to the degree of unevenness of the subject's cheek was obtained by taking a skin image using a skin image analysis counseling system (VISIA-EVOLUTION manufactured by Canfield) and analyzing the image.

被験者の目立つ毛穴個数の少なさに関するパラメータは、皮膚画像解析カウンセリングシステム（Canfield社製、ＶＩＳＩＡ－ＥＶＯＬＵＴＩＯＮ）を用いて皮膚画像を採取し、画像解析することにより得た。 A parameter relating to the number of conspicuous pores of a subject was obtained by taking a skin image using a skin image analysis counseling system (VISIA-EVOLUTION manufactured by Canfield) and analyzing the image.

被験者の紅斑指数の低さに関するパラメータは、分光測色計（コニカミノルタセンシング（株）製、ＣＭ２６００ｄ）を用いて、吸光度（Ａｂｓｏｒｂａｎｃｅ：Ａ）を測定し、その後、紅斑指数（ＥＩ）算出式（ＥＩ＝｛［Ａ_５６０＋１．５（Ａ_５４３）＋Ａ_５７６］－２．０（Ａ_５１０＋Ａ_６１０）｝、Ｆｅａｔｈｅｒの公式）を用いて紅斑指数を算出・評価することにより得た。 The parameters related to the low erythema index of the subject were measured using a spectrophotometer (Konica Minolta Sensing Co., Ltd., CM2600d), absorbance (Absorbance: A), and then the erythema index (EI) calculation formula ( EI={[A ₅₆₀ +1.5(A ₅₄₃ )+A ₅₇₆ ]−2.0(A ₅₁₀ +A ₆₁₀ )}, Feather's formula) was used to calculate and evaluate the erythema index.

被験者のキメ状態の良さに関するパラメータは、シリコーンラバー等の樹脂を用いて、肌表面形状を写し取る等の手法でレプリカを作成し、予め定めたスコア（例えば１０段階）に照らし合わせることで得た。 Parameters related to the texture condition of the subject were obtained by creating a replica using a method such as copying the skin surface shape using a resin such as silicone rubber and comparing it with a predetermined score (eg, 10 levels).

被験者の皮脂量の少なさに関するパラメータは、Sebumeter ＳＭ８１５を用いて、洗顔後約４０分後の額と頬部の皮脂量を各１回ずつ測定し、「回復皮脂量」を評価することにより得た。 The parameters related to the low sebum amount of the subject were obtained by measuring the amount of sebum on the forehead and cheeks about 40 minutes after washing the face using Sebumeter SM815 and evaluating the "restored sebum amount". rice field.

被験者のシワ本数の少なさに関するパラメータは、皮膚画像解析カウンセリングシステム（Canfield社製、ＶＩＳＩＡ－ＥＶＯＬＵＴＩＯＮ）を用いて皮膚画像を採取し、画像解析することにより得た。 A parameter relating to the number of wrinkles in the subject was obtained by collecting skin images using a skin image analysis counseling system (VISIA-EVOLUTION, manufactured by Canfield) and analyzing the images.

被験者のメラニン指数の低さに関するパラメータは、分光測色計（コニカミノルタセンシング（株）製、ＣＭ２６００ｄ）を用いて、吸光度（Ａｂｓｏｒｂａｎｃｅ：Ａ）を測定し、その後、メラニン指数（ＭＩ）算出式（ＭＩ＝Ａ_６４０－Ａ_６７０、Ｆｅａｔｈｅｒの公式）を用いてメラニン指数を算出し、評価することにより得た。 Parameters related to the low melanin index of the subject, using a spectrophotometer (manufactured by Konica Minolta Sensing Co., Ltd., CM2600d), absorbance (Absorbance: A) is measured, then the melanin index (MI) calculation formula ( MI=A ₆₄₀ -A ₆₇₀ , Feather's formula) was used to calculate and evaluate the melanin index.

（２）被験者群の肌状態によるグループ分け
次に前述の被験者全データを計算し、測定値が下位１０％であった被験者群と、測定値が上位１０％であった被験者群とに、グループ分けを行った。 (2) Grouping by skin condition of the subject group Next, all the above-mentioned subject data are calculated, and the subject group with the lowest 10% measured value and the subject group with the highest 10% measured value are divided into groups. made a division.

（３）被験者の皮膚常在細菌叢の採取、解析
同時に、被験者の女性頬部から菌採取用のシール又は綿棒（Swab法）にて、細菌叢を採取した。次に、採取した細菌叢よりDNAの抽出を行い、細菌が保有する１６ＳｒＲＮＡＶ１－Ｖ２領域をＰＣＲにて増幅した。ｑＰＣＲにより各サンプルの濃度を計測した後ランを行う全てのサンプルを混合、ライブラリ調整を経てMiseqでのランを行った。得られたDNA配列をNCBIのデータベースと照合し、菌種を同定した。 (3) Collection and Analysis of Indigenous Skin Microflora of Subjects At the same time, bacterial flora was collected from female cheeks of subjects using a seal or a cotton swab (Swab method) for collection of bacteria. Next, DNA was extracted from the collected bacterial flora, and the 16S rRNA V1-V2 region possessed by the bacteria was amplified by PCR. After measuring the concentration of each sample by qPCR, all the samples to be run were mixed, and after library preparation, the run with Miseq was carried out. The obtained DNA sequences were collated with the NCBI database to identify the strain.

同定した菌種を、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される由来環境と照合し、菌が検出された環境のうち該菌の平均存在比率の最も高い環境（図１参照）を特定した。 The identified bacterial strains are compared with the origin environment specified in the National Institute of Genetics bacterial database (MicrobeDB.jp), and the average abundance ratio of the bacteria is the highest among the environments where the bacteria were detected. We identified a high environment (see Figure 1).

（４）肌状態によってグループ分けした被験者群と、皮膚常在細菌叢における菌の由来環境の相関についての統計解析
前述の（２）の工程で肌状態によってグループ分けした被験者群のうち、肌状態が良好であった被検者群と、前述の（３）の工程で算出した各環境由来菌の菌量の相関を統計解析した。統計解析した結果のうち、グループ分けした被験者群間で菌量に有意な差のあった環境由来菌を選び出した。ここで、菌量の計測は、ＯＴＵ（ＯｐｅｒａｔｉｏｎａｌＴａｘｏｎｏｍｉｃＵｎｉｔ）解析を行い、各ＯＴＵに属するリード数を計測することにより、行った。 (4) Statistical analysis of the correlation between the subject group grouped by skin condition and the environment of the origin of bacteria in the skin resident flora Among the subject groups grouped by skin condition in the above step (2), Statistical analysis was performed on the correlation between the test subject group in which the test was good and the amount of each environment-derived bacteria calculated in the above step (3). From the results of statistical analysis, environment-derived bacteria with significant differences in the amount of bacteria among the grouped subject groups were selected. Here, the amount of bacteria was measured by performing OTU (Operational Taxonomic Unit) analysis and counting the number of reads belonging to each OTU.

（５）菌量の計測
選び出した環境由来菌について、肌状態が良好な被験者群の皮膚常在細菌叢における菌量を計測した。
結果を図２～図１４に示す。なお、各図においては選び出した環境由来菌、すなわち被験者群間で菌量に有意な差のあった環境由来菌についてのみ、棒グラフによりその菌量を示している。被験者群間で菌量に有意な差のなかった環境由来菌については、棒グラフによる菌量の表示を省略している。 (5) Measurement of Bacteria Amount For the selected environment-derived bacteria, the amount of bacteria in the indigenous skin flora of a group of subjects with good skin conditions was measured.
The results are shown in FIGS. 2-14. In each figure, bar graphs show the amount of selected environment-derived bacteria, that is, only the environment-derived bacteria having significant differences in the amount of bacteria between subject groups. For environmental bacteria for which there was no significant difference in the amount of bacteria between subject groups, the display of the amount of bacteria in the bar graph is omitted.

［考察］
図２の結果より、ＴＥＷＬ（経皮水分蒸散量）の良好な被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＡ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ａ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［animal associated］、［animal body associated］、［land feature］ [Discussion]
From the results in Fig. 2, in the group of subjects with good TEWL (lowest 10% skin measurement value), in the bacteria database (MicrobeDB.jp) of the National Institute of Genetics It was found that the amount of environment-derived bacteria derived from the environment selected from the following defined group A was large.
(Group A)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [animal associated], [animal body associated], [land feature]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＡ群の環境のうち、１又は２以上の環境に由来する菌の菌量が多いほど、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態が好ましいと判定することができることがわかった。 That is, the larger the amount of bacteria derived from one or two or more of the Group A environments defined in the National Institute of Genetics bacterial database (MicrobeDB.jp), It was found that the skin condition based on the TEWL (transepidermal water loss) parameter can be determined to be preferable.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＡ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、ＴＥＷＬ（経皮水分蒸散量）のパラメータに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the group A more than specified in the National Institute of Genetics bacteria database (MicrobeDB.jp) It can be said that the substance or cosmetic method that increases the amount of bacteria is the substance or cosmetic method that can improve the skin condition based on the parameter of TEWL (transepidermal water loss).

図３の結果より、皮膚の明るさ（Ｌ^＊）の良好な被験者群（肌測定値上位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＢ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｂ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［land feature］ From the results of FIG. 3, the subject group with good skin brightness (L ^* ) (top 10% group of skin measurements) has the National Institute of Genetics (MicrobeDB.jp) It was found that the amount of environment-derived bacteria derived from the environment selected from the following defined group B was large.
(Group B)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [land feature]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＢ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚の明るさ（Ｌ^＊）のパラメータに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria selected from the environment-derived bacteria selected from more than B group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the skin condition based on the skin brightness (L ^* ) parameter can be determined to be more favorable as the amount of bacteria increases.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＢ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、皮膚の明るさ（Ｌ^＊）のパラメータに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from more than Group B specified in the National Institute of Genetics bacteria database (MicrobeDB.jp) A substance or cosmetic method that increases the amount of bacteria can be said to be a substance or cosmetic method that can improve the skin condition based on the skin brightness (L ^* ) parameter.

図４の結果より、肌の弾力性（Ｕｒ／Ｕｆ）の良好な被験者群（肌測定値上位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＣ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｃ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［park associated］、［land area］ From the results of FIG. 4, the group of subjects with good skin elasticity (Ur/Uf) (top 10% group of skin measurement values) had the bacteria database (MicrobeDB.jp) of the National Institute of Genetics. It was found that the amount of environment-derived bacteria derived from the environment selected from the following group C defined in is large.
(Group C)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [park associated], [land area]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＣ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、肌の弾力性（Ｕｒ／Ｕｆ）のパラメータに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria derived from the environment selected from the above group C specified in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the skin condition based on the skin elasticity (Ur/Uf) parameter can be determined to be more favorable as the amount of bacteria increases.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＣ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、肌の弾力性（Ｕｒ／Ｕｆ）のパラメータに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the above group C specified in the National Institute of Genetics bacterial database (MicrobeDB.jp) It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on the parameters of skin elasticity (Ur/Uf).

図５の結果より、シミの少ない被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＤ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｄ群）
［plant body associated 若しくは symbiotic microbe］、［water associated］、［animal associated］、［animal body associated］、［land feature］ From the results of FIG. 5, the subject group with few spots (lower 10% group of skin measurement values) was selected from the following D group defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) It was found that the amount of environment-derived bacteria derived from the selected environment was large.
(Group D)
[plant body associated or symbiotic microbe], [water associated], [animal associated], [animal body associated], [land feature]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＤ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、シミの少なさに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria selected from the environment-derived bacteria selected from the D group more than specified in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the higher the amount of bacteria, the more favorable the skin condition based on the lesser number of spots can be determined.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＤ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、シミの少なさに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the D group more than specified in the National Institute of Genetics bacteria database (MicrobeDB.jp) It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on less spots.

図６の結果より、角層水分量の多い被験者群（肌測定値上位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＥ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｅ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］ From the results of FIG. 6, the subject group with a high stratum corneum moisture content (top 10% group of skin measurement values) has the following stipulated in the National Institute of Genetics bacterial database (MicrobeDB.jp). It was found that the amount of environment-derived bacteria derived from the environment selected from the E group was large.
(Group E)
[plant body associated or symbiotic microbe], [agriculture associated]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＥ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、角層水分量の多さに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria derived from the environment selected from the E group more than specified in the National Institute of Genetics bacterial database (MicrobeDB.jp) It was found that the higher the amount of bacteria, the more favorable the skin condition based on the higher moisture content of the stratum corneum can be determined.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＥ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、角層水分量の多さに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the E group more than those specified in the National Institute of Genetics bacterial database (MicrobeDB.jp) It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on the high moisture content of the stratum corneum.

図７の結果より、黄味の少ない被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される［plant body associated 若しくは symbiotic microbe］の環境に由来する環境由来菌の菌量が多いことがわかった。 From the results of FIG. 7, the subject group with less yellowness (bottom 10% group of measured skin values) was defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) [plant body associated or symbiotic microbe].

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される［plant body associated 若しくは symbiotic microbe］の環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、黄味の少なさに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria derived from the environment of [plant body associated or symbiotic microbe] defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the skin condition based on less yellowness can be determined to be more favorable as the amount of bacteria increases.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される［plant body associated 若しくは symbiotic microbe］の環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、黄味の少なさに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment of [plant body associated or symbiotic microbe] defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on less yellowness.

図８の結果より、頬部の凹凸の少ない被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＦ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｆ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［park associated］、［land area］、［land feature］、［fungi body associated］ From the results of FIG. 8, the subject group with less uneven cheeks (bottom 10% of skin measurement values) had the following bacteria database (MicrobeDB.jp) of the National Institute of Genetics. It was found that the amount of bacteria derived from the environment selected from group F was large.
(F group)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [animal body associated], [park associated], [ land area], [land feature], [fungi body associated]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＦ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、頬部の凹凸の少なさに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria derived from the environment selected from the F group more than those specified in the National Institute of Genetics bacterial database (MicrobeDB.jp). It was found that the skin condition based on less unevenness of the cheek can be determined to be more favorable as the amount of bacteria increases.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＦ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、頬部の凹凸の少なさに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the F group more than those specified in the National Institute of Genetics bacterial database (MicrobeDB.jp). It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on less unevenness of the cheek.

図９の結果より、目立つ毛穴個数の少ない被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＧ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｇ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［aquatic feature］、［animal associated］、［animal body associated］、［park associated］、［land area］、［land feature］ From the results of FIG. 9, the subject group with a small number of conspicuous pores (bottom 10% group of skin measurement values) had the following G defined in the National Institute of Genetics bacterial database (MicrobeDB.jp). It was found that the amount of environment-derived bacteria derived from the environment selected from the group was large.
(Group G)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [animal body associated], [park associated], [ land area], [land feature]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＧ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、目立つ毛穴個数の少なさに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria derived from the environment selected from the G group more than specified in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the more the amount of bacteria, the more favorable the skin condition based on the smaller number of conspicuous pores.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＧ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、目立つ毛穴個数の少なさに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the G group more than those specified in the National Institute of Genetics bacterial database (MicrobeDB.jp). It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on the smaller number of conspicuous pores.

図１０の結果より、紅斑指数の低い被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＨ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｈ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］ From the results of FIG. 10, the subject group with low erythema index (bottom 10% skin measurement value group) includes the following H group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the amount of environment-derived bacteria derived from the environment selected from is large.
(Group H)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [animal body associated], [land feature]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＨ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、紅斑指数の低さに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria derived from the environment selected from the H group more than those specified in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the skin condition based on the lower erythema index can be determined to be more favorable as the amount of bacteria increases.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＨ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、紅斑指数の低さに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the H group more than those specified in the National Institute of Genetics bacterial database (MicrobeDB.jp). A substance or cosmetic method that increases the amount of bacteria can be said to be a substance or cosmetic method that can improve the skin condition based on a low erythema index.

図１１の結果より、キメ状態の良い被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＩ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｉ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［water associated］、［animal associated］、［land area］、［land feature］、［fungi body associated］ From the results of FIG. 11, the subject group with good texture (bottom 10% group of skin measurement values) includes the following group I defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) It was found that the amount of environment-derived bacteria derived from the environment selected from is large.
(Group I)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [land area], [land feature], [fungi body associated]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＩ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、キメ状態の良さに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria derived from the environment selected from Group I above those defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) It was found that the higher the amount of bacteria, the more favorable the skin condition based on the good texture condition can be determined.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＩ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、キメ状態の良さに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from Group I above those specified in the National Institute of Genetics bacterial database (MicrobeDB.jp) It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on the better skin texture.

図１２の結果より、皮脂量の少ない被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＪ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｊ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［aquatic area 若しくは fluid in state］、［water associated］、［animal associated］、［animal body associated］、［land area］、［land feature］ From the results of FIG. 12, the subject group with a small amount of sebum (lower 10% group of skin measurement values) has the following group J defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the amount of environment-derived bacteria derived from the environment selected from is large.
(Group J)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [animal associated], [animal body associated], [land area], [land feature]

すなわち、皮脂量の少ない被験者群には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＪ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮脂量の少なさに基づく肌状態が好ましいと判定することができることがわかった。 That is, in the subject group with a small amount of sebum, among the environment-derived bacteria derived from the environment selected from the J group more than specified in the bacteria database (MicrobeDB.jp) of the National Institute of Genetics, It was found that the higher the amount of one or more bacteria derived from the environment, the more favorable the skin condition based on the lower amount of sebum can be determined.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＪ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、皮脂量の少なさに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria selected from the environment-derived bacteria selected from Group J above those specified in the National Institute of Genetics bacteria database (MicrobeDB.jp) It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on a small amount of sebum.

図１３の結果より、シワ本数の少ない被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＫ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｋ群）
［plant body associated 若しくは symbiotic microbe］、［agriculture associated］、［animal associated］、［animal body associated］、［land feature］ From the results of FIG. 13, the subject group with a small number of wrinkles (lower 10% group of skin measurement values) has the following K groups defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) It was found that the amount of environment-derived bacteria derived from the environment selected from is large.
(K group)
[plant body associated or symbiotic microbe], [agriculture associated], [animal associated], [animal body associated], [land feature]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＫ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、シワ本数の少なさに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or two or more of the environment-derived bacteria selected from the K group more than those specified in the National Institute of Genetics bacterial database (MicrobeDB.jp) It was found that the skin condition based on the smaller number of wrinkles can be determined to be more favorable as the amount of bacteria increases.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＫ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、シワ本数の少なさに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the K group more than specified in the National Institute of Genetics bacterial database (MicrobeDB.jp) It can be said that a substance or a cosmetic method that increases the amount of bacteria is a substance or a cosmetic method that can improve the skin condition based on fewer wrinkles.

図１４の結果より、メラニン指数の低い被験者群（肌測定値下位10％群）には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以下のＬ群から選ばれる環境に由来する環境由来菌の菌量が多いことがわかった。
（Ｌ群）
［agriculture associated］、［water associated］、［animal associated］、［animal body associated］、［land feature］ From the results of FIG. 14, the group of subjects with low melanin index (bottom 10% group of measured skin values) includes the following L group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the amount of environment-derived bacteria derived from the environment selected from is large.
(L group)
[agriculture associated], [water associated], [animal associated], [animal body associated], [land feature]

すなわち、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＬ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、メラニン指数の低さに基づく肌状態が好ましいと判定することができることがわかった。 That is, one or more of the environment-derived bacteria derived from the environment selected from the L group more than defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It was found that the skin condition based on the low melanin index can be determined to be more favorable as the amount of bacteria increases.

さらに、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される以上のＬ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量を増加させる物質若しくは美容方法ほど、メラニン指数の低さに基づく肌状態を改善することができる物質若しくは美容方法であるといえる。 Furthermore, one or more of the environment-derived bacteria derived from the environment selected from the L group more than defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) It can be said that the substance or cosmetic method that increases the amount of bacteria is the substance or cosmetic method that can improve the skin condition based on the low melanin index.

＜試験２＞皮膚常在細菌叢における菌の多様性と、各肌状態との関連
以下の肌状態に関するパラメータと皮膚表面の細菌叢との関連性を調べた。なお、以下、に記載の菌量は、特に断りのない限り、各細菌のリード数でありその細菌種の存在量を表す。 <Test 2> Diversity of Bacteria in Indigenous Skin Microflora and Relevance to Each Skin Condition The relationship between the following skin condition-related parameters and skin surface microflora was investigated. Unless otherwise specified, the amount of bacteria described below is the number of reads for each bacterium and represents the abundance of that bacterium species.

（１）被験者の肌状態の測定
被験者となる女性は３０代のアトピーなどの疾患を有しない健常日本人女性２９３名である。
まず、被験者の肌状態に関するパラメータの取得を行った。取得したパラメータは、目立つ毛穴個数の少なさ、皮脂量の少なさ、紅斑指数の低さ、ＴＥＷＬ（経皮水分蒸散量）、メラニン指数の低さ、皮膚の明るさ（Ｌ^＊）である。
なお、被験者の肌状態に関するパラメータは、試験１と同様の方法により、取得した。 (1) Measurement of Skin Conditions of Test Subjects The women to be tested are 293 healthy Japanese women in their 30s who do not have diseases such as atopy.
First, parameters related to the subject's skin condition were acquired. The parameters obtained are: low number of conspicuous pores, low sebum content, low erythema index, TEWL (transepidermal water loss), low melanin index, and skin lightness (L ^* ).
Parameters related to the subject's skin condition were obtained by the same method as in Test 1.

（３）被験者の皮膚常在細菌叢の採取、解析
次に、試験１と同様の方法により被験者の女性頬部の細菌叢を取得し、菌種の同定を行った。 (3) Sampling and Analysis of Indigenous Skin Microflora of Subjects Next, bacterial flora of female cheeks of subjects were obtained by the same method as in Test 1, and bacterial species were identified.

同定した菌種に関する情報に基づき、細菌の多様性を示すＳｉｍｐｓｏｎｉｎｄｅｘを算出した。
（４）肌状態によってグループ分けした被験者群と、細菌叢における菌に関するパラメータとの相関についての統計解析
前述の（２）の工程で肌状態によってグループ分けした被験者群と、前述の（３）の工程で算出した各細菌種の占有率及びＳｉｍｐｓｏｎｉｎｄｅｘの相関を統計解析した。
結果を図１５～図２０に示す。 Based on the information on the identified bacterial species, the Simpson index, which indicates the diversity of bacteria, was calculated.
(4) Statistical analysis of correlation between subject groups grouped by skin condition and parameters related to bacteria in bacterial flora The correlation between the occupancy rate of each bacterial species calculated in the process and the Simpson index was statistically analyzed.
The results are shown in FIGS. 15-20.

［結果・考察］
図１５の結果より、目立つ毛穴個数の少ない被験者群は、目立つ毛穴個数の多い被験者群に比して、皮膚常在細菌叢の菌種の多様性に優れていることがわかった。
試験１の図９の結果より、目立つ毛穴個数の少ない被験者群には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＧ群から選ばれる環境に由来する環境由来菌の菌量が多い。
すなわち、上記のＧ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると判定することができることがわかった。
また、皮膚常在細菌叢における多様性に富んでいるほど、上記のＧ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いと判定することができることがわかった。 [Results/Discussion]
From the results of FIG. 15, it was found that the subject group with a small number of conspicuous pores was superior in diversity of bacterial species of the indigenous skin flora to the subject group with a large number of conspicuous pores.
From the results of FIG. 9 of Test 1, the subject group with a small number of conspicuous pores was selected from the above G group defined in the National Institute of Genetics bacterial database (MicrobeDB.jp). The amount of bacteria derived from the environment is large.
That is, among the environmental bacteria derived from the environment selected from the above group G, the greater the amount of one or two or more environment-derived bacteria, the more diverse the indigenous skin flora. It turns out that it can be done.
In addition, it is determined that the more diverse the indigenous skin flora, the greater the amount of one or more of the environment-derived bacteria derived from the environment selected from the above G group. It turns out that it can be done.

図１６の結果より、皮脂量の少ない被験者群は、皮脂量の多い被験者群に比して、皮膚常在細菌叢の菌種の多様性に優れていることがわかった。
試験１の図１２の結果より、皮脂量の少ない被験者群には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＪ群から選ばれる環境に由来する環境由来菌の菌量が多い。
すなわち、上記のＪ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると判定することができることがわかった。
また、皮膚常在細菌叢における多様性に富んでいるほど、上記のＪ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いと判定することができることがわかった。 From the results of FIG. 16, it was found that the subject group with a small amount of sebum was superior to the subject group with a large amount of sebum in terms of the diversity of bacterial species of the indigenous skin flora.
From the results of FIG. 12 of Test 1, the subject group with a small amount of sebum is derived from the environment selected from the above group J defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) There is a large amount of bacteria derived from the environment.
That is, among the environment-derived bacteria derived from the environment selected from the above group J, the greater the amount of one or more environment-derived bacteria, the more diverse the indigenous skin flora. It turns out that it can be done.
In addition, it is determined that the more diverse the indigenous skin flora, the greater the amount of one or more environment-derived bacteria among the environment-derived bacteria selected from the above group J. It turns out that it can be done.

図１７の結果より、紅斑指数の低い被験者群は、紅斑指数の高い被験者群に比して、皮膚常在細菌叢の菌種の多様性に優れていることがわかった。
試験１の図１０の結果より、紅斑指数の低い被験者群には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＨ群から選ばれる環境に由来する環境由来菌の菌量が多い。
すなわち、上記のＨ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると判定することができることがわかった。
また、皮膚常在細菌叢における多様性に富んでいるほど、上記のＨ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いと判定することができることがわかった。 From the results of FIG. 17, it was found that the test subject group with a low erythema index was superior to the test subject group with a high erythema index in diversity of bacterial species of the indigenous skin flora.
From the results of FIG. 10 of Test 1, the subject group with a low erythema index is derived from the environment selected from the above H group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) There is a large amount of bacteria derived from the environment.
That is, among the environmental bacteria derived from the environment selected from the above group H, the greater the amount of one or more environment-derived bacteria, the more diverse the indigenous skin flora. It turns out that it can be done.
In addition, it is determined that the more diverse the indigenous skin flora, the greater the amount of one or more environment-derived bacteria among the environment-derived bacteria selected from the above H group. It turns out that it can be done.

図１８の結果より、ＴＥＷＬ（経皮水分蒸散量）の良好な被験者群は、ＴＥＷＬ（経皮水分蒸散量）の良くない被験者群に比して、皮膚常在細菌叢の菌種の多様性に優れていることがわかった。
試験１の図２の結果より、ＴＥＷＬ（経皮水分蒸散量）の良好な被験者群には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＡ群から選ばれる環境に由来する環境由来菌の菌量が多い。
すなわち、上記のＡ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると判定することができることがわかった。
また、皮膚常在細菌叢における多様性に富んでいるほど、上記のＡ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いと判定することができることがわかった。 From the results of FIG. 18, the group of subjects with good TEWL (transepidermal water loss) has a higher diversity of bacterial species in the skin flora than the group of subjects with poor TEWL (transepidermal water loss). was found to be superior to
From the results of FIG. 2 of Test 1, the subject group with good TEWL (transepidermal water loss) has the above A defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) The amount of environment-derived bacteria derived from the environment selected from the group is large.
That is, among the environment-derived bacteria derived from the environment selected from the above group A, the greater the amount of one or more environment-derived bacteria, the more diverse the indigenous skin flora. It turns out that it can be done.
In addition, it is determined that the more diverse the indigenous skin flora, the greater the amount of one or more environment-derived bacteria among the environment-derived bacteria selected from the above group A. It turns out that it can be done.

図１９の結果より、メラニン指数の低い被験者群は、メラニン指数の高い被験者群に比して、皮膚常在細菌叢の菌種の多様性に優れていることがわかった。
試験１の図１４の結果より、メラニン指数の低い被験者群には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＬ群から選ばれる環境に由来する環境由来菌の菌量が多い。
すなわち、上記のＬ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると判定することができることがわかった。
また、皮膚常在細菌叢における多様性に富んでいるほど、上記のＬ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いと判定することができることがわかった。 From the results of FIG. 19, it was found that the test subject group with a low melanin index was superior to the test subject group with a high melanin index in diversity of bacterial species of the indigenous skin flora.
From the results of FIG. 14 of Test 1, the subject group with low melanin index is derived from the environment selected from the above L group defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) There is a large amount of bacteria derived from the environment.
That is, among the environmental bacteria derived from the environment selected from the above group L, the larger the amount of one or more environment-derived bacteria, the more diverse the indigenous skin flora. It turns out that it can be done.
In addition, it is determined that the more diverse the indigenous skin flora, the greater the amount of one or more environment-derived bacteria among the environment-derived bacteria selected from the above group L. It turns out that it can be done.

図２０の結果より、皮膚の明るさ（Ｌ^＊）の良好な被験者群は、皮膚の明るさ（Ｌ^＊）の良くない被験者群に比して、皮膚常在細菌叢の菌種の多様性に優れていることがわかった。
試験１の図３の結果より、メラニン指数の低い被験者群には、国立遺伝研究所（National Institute of Genetics）の細菌データベース（MicrobeDB.jp）において規定される上記のＢ群から選ばれる環境に由来する環境由来菌の菌量が多い。
すなわち、上記のＢ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いほど、皮膚常在細菌叢の多様性に富んでいると判定することができることがわかった。
また、皮膚常在細菌叢における多様性に富んでいるほど、上記のＢ群から選ばれる環境に由来する環境由来菌のうち、１又は２以上の環境由来菌の菌量が多いと判定することができることがわかった。 From the results of FIG. 20, the group of subjects with good skin brightness (L ^* ) has more diversity in the bacterial species of the skin flora than the group of subjects with poor skin brightness (L ^* ). was found to be superior to
From the results of FIG. 3 of Test 1, the subject group with low melanin index is derived from the environment selected from the above B group defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) There is a large amount of bacteria derived from the environment.
That is, among the environment-derived bacteria selected from the above-mentioned group B, the greater the amount of one or more environment-derived bacteria, the more diverse the indigenous skin flora. It turns out that it can be done.
In addition, it is determined that the more diverse the indigenous skin flora, the greater the amount of one or more environment-derived bacteria among the environment-derived bacteria selected from the above group B. It turns out that it can be done.

Claims

A method for diagnosing a specific skin condition, comprising:
a bacterial species identification step of identifying bacterial species present in the skin flora;
After collating the identified bacterial strains in a database having information on environment-derived bacteria and specifying the origin environment of the bacterial species, the amount of specific environment-derived bacteria in the skin flora that correlates with the specific skin condition. As a measurement step of measuring the total amount of bacteria in the same origin environment ,
a determination step of determining that the specific skin condition is more favorable as the amount of the specific environment-derived bacteria increases, based on the measurement results;
has
The environment-derived bacterium information is information that links information on the bacterium species or genus with information on the environment in which the bacterium species or genus is detected ,
A diagnostic method , wherein the origin environment is an environment having the highest average abundance ratio of the bacterial species or the bacterial genus among the environments in which the bacterial species or the bacterial genus defined in the database are detected .

the specific skin condition is based on a TEWL (transepidermal water loss) parameter;
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following group A defined in the bacterium database (MicrobeDB.jp) of the National Institute of Genetics .
(Group A)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [animal associated], [animal body associated], [land feature]

wherein the specific skin condition is based on a skin lightness (L*) parameter;
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following group B defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) .
(Group B)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [land feature]

wherein the specific skin condition is based on a skin elasticity (Ur/Uf) parameter;
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following group C defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) .
(Group C)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [park associated], [land area]

The specific skin condition is based on less spots,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following group D defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) .
(Group D)
[plant body associated or symbiotic microbe], [water associated], [animal associated], [animal body associated], [land feature]

The specific skin condition is based on a large stratum corneum moisture content,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following group E defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) .
(Group E)
[plant body associated or symbiotic microbe], [agriculture associated]

The specific skin condition is based on less yellowness of the skin,
The diagnostic method according to claim 1, wherein the origin environment is [plant body associated or symbiotic microbe ] defined in the National Institute of Genetics bacterial database (MicrobeDB.jp). .

The specific skin condition is based on less unevenness of the cheeks,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following F groups defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) .
(F group)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [animal body associated], [park associated], [ land area], [land feature], [fungi body associated]

It is based on the small number of pores that make the specific skin condition conspicuous,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following G groups defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) .
(Group G)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [aquatic feature], [animal associated], [animal body associated], [park associated], [ land area], [land feature]

The specific skin condition is based on a low erythema index,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following H group defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) .
(Group H)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [animal body associated], [land feature]

The specific skin condition is based on a good texture condition,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following group I defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) .
(Group I)
[plant body associated or symbiotic microbe], [agriculture associated], [water associated], [animal associated], [land area], [land feature], [fungi body associated]

The specific skin condition is based on a small amount of sebum,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following group J defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) .
(Group J)
[plant body associated or symbiotic microbe], [agriculture associated], [aquatic area or fluid in state], [water associated], [animal associated], [animal body associated], [land area], [land feature]

The specific skin condition is based on a small number of wrinkles,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following K groups defined in the National Institute of Genetics bacterial database (MicrobeDB.jp) .
(K group)
[plant body associated or symbiotic microbe], [agriculture associated], [animal associated], [animal body associated], [land feature]

The specific skin condition is based on a low melanin index,
2. The diagnostic method according to claim 1, wherein the environment of origin is selected from the following group L defined in the National Institute of Genetics bacteria database (MicrobeDB.jp) .
(L group)
[agriculture associated], [water associated], [animal associated], [animal body associated], [land feature]

A method for estimating the diversity of skin flora, comprising:
a bacterial species identification step of identifying bacterial species present in the skin flora;
After collating the identified bacterial species against a database having information on environment-derived bacteria and specifying the environment of origin of the bacterial species, the amount of specific environment-derived bacteria that correlates with a specific skin condition in the skin flora , a measurement step of measuring the total amount of bacteria in the same origin environment ;
a determination step of determining, based on the measurement results, that the greater the amount of the specific environment-derived bacteria, the more diverse the indigenous skin microflora;
The environment-derived bacterium information is information that links information on the bacterium species or genus with information on the environment in which the bacterium species or genus is detected,
The origin environment is the environment with the highest average abundance ratio of the bacterial species or genus among the environments where the bacterial species or genus defined in the database is detected,
An estimation method, wherein the specific skin condition is a skin condition correlated with the diversity.

A diversity analysis step of analyzing diversity in skin flora,
a determination step of determining, based on the analysis results, that the greater the diversity, the greater the amount of specific environment-derived bacteria correlated with a specific skin condition in the indigenous skin flora;
has
The specific skin condition is a skin condition correlated with the diversity ,
The amount of the specific environment-derived bacteria is the total amount of bacteria from the same environment of origin,
The environment of origin is the environment with the highest average abundance ratio of the bacterial species or genus among the environments in which the bacterial species or genus defined in the database provided with the environmental bacteria information is detected,
The resident skin flora, characterized in that the environment-derived bacteria information is information that associates information on a bacterial species or genus with information on the environment in which the bacterial species or genus is detected. Method for estimating the amount of the specific environment-derived bacteria in the method.

A method for analyzing the attributes of bacteria correlated with a specific skin condition,
an identifying step of identifying the species or genus of bacteria present in the indigenous skin flora of the specific group of subjects with good skin condition and the group of subjects with poor skin condition, which have a difference in the amount of bacteria between the two groups;
a step of obtaining information on the attributes of the bacterial species or genus identified in the identification step;
The attribute is the origin environment of the bacterium identified in the identification step,
The environment of origin is the environment with the highest average abundance ratio of the bacterial species or genus among the environments in which the bacterial species or genus defined in the database provided with the environmental bacteria information is detected,
The analysis method, wherein the environment-derived bacterial information is information in which information relating to a bacterial species or genus is associated with information relating to the environment in which the bacterial species or genus is detected .

A screening method for a substance or cosmetic method having an effect of improving skin condition,
It is characterized by determining that the more the amount of bacteria derived from a specific environment that is correlated with a specific skin condition, the more excellent the effect of improving the skin condition ,
The amount of the specific environment-derived bacteria is the total amount of bacteria from the same environment of origin,
The environment of origin is the environment with the highest average abundance ratio of the bacterial species or genus among the environments in which the bacterial species or genus defined in the database provided with the environmental bacteria information is detected,
The environment-derived bacterium information is information that links information on the bacterium species or genus with information on the environment in which the bacterium species or genus is detected, and is a substance or cosmetic that has an action to improve skin conditions. Method screening method.

The diagnostic method according to claim 1, wherein said database is the National Institute of Genetics bacteria database (MicrobeDB.jp).