JP7166348B2 - 細胞解析装置及び細胞解析方法 - Google Patents
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Description
電子線又はX線照射元素分析法によりリンのシグナル強度又はリン及びイオウのシグナル強度を測定する元素分析部と、
対照のリンのシグナル強度、又は対照のリン及びイオウのシグナル強度若しくはリンとイオウの濃度比が登録されているデータベースと、
前記元素分析部で測定されるリンのシグナル強度に応じた細胞分布と、前記データベースに登録された対照のリンのシグナル強度に応じた細胞分布とを比較する比較部、又は前記元素分析部で測定されるリン及びイオウのシグナル強度から算出されたリンとイオウの濃度比と、前記データベースに登録された対照のリンとイオウの濃度比を比較する比較部と
を備える。
Claims (13)
- 複数の細胞を含む試料について、走査電子顕微鏡(SEM)画像を取得する工程と、
前記SEM画像から、前記細胞の核領域を選択する工程と、
電子線又はX線照射元素分析法により前記細胞ごとの前記核領域におけるリンのシグナル強度を測定する工程と、
測定した前記試料の細胞ごとのリンのシグナル強度に応じた細胞分布を、対照のシグナル強度に応じた細胞分布と比較する工程と
を含むことを特徴とする細胞の増殖活性又は悪性度を解析する方法。 - 前記試料が、支持基板に接着した組織切片又は細胞である、請求項1に記載の方法。
- 前記試料が臓器又は細胞集団由来の複数の細胞を含む、請求項1又は2に記載の方法。
- 前記対照が、正常細胞、悪性細胞、増殖活性の高い細胞、及びDNAの異常増加を示す細胞からなる群より選択される少なくとも1種を含む、請求項1~3のいずれか1項に記載の方法。
- 前記対照が正常細胞を含む場合、前記対照よりもリンのシグナル強度の高い細胞の割合と、リンのシグナル強度の範囲を決定する工程をさらに含む、請求項1に記載の方法。
- 前記SEM画像を取得する工程の前に、前記試料を染色して光学顕微鏡で観察する工程、及び前記試料の画像を取得する範囲を選択する工程をさらに含む、請求項1~5のいずれか1項に記載の方法。
- 前記試料を染色して光学顕微鏡画像を取得する工程、
前記試料の走査電子顕微鏡(SEM)画像を取得する工程、
前記SEM画像において、前記測定した細胞ごとのリンのシグナル強度に応じて細胞を強調表示する工程、
前記SEM画像を、前記光学顕微鏡画像と照合することにより、前記光学顕微鏡画像において、前記測定した細胞ごとのリンのシグナル強度に応じて細胞を強調表示する工程
をさらに含む、請求項1に記載の方法。 - 試料ががん細胞を含むか否かを判定する工程をさらに含む、請求項1~7のいずれか1項に記載の方法。
- 複数の細胞を含む試料の走査電子顕微鏡(SEM)画像を取得する画像取得部と、
前記SEM画像から前記細胞の核領域を選択する選択部と、
電子線又はX線照射元素分析法により前記細胞ごとのリンのシグナル強度を測定する元素分析部と、
対照のリンのシグナル強度が登録されているデータベースと、
前記元素分析部で測定される細胞ごとのリンのシグナル強度に応じた細胞分布と、前記データベースに登録された対照のリンのシグナル強度に応じた細胞分布とを比較する比較部と
を備え、前記元素分析部は、前記選択部で選択された核領域におけるリンのシグナル強度を測定することを特徴とする細胞の増殖活性又は悪性度の解析装置。 - 元素分析部が、エネルギー分散型X線分光器(EDS)、波長分散型X線分光器(WDS)、又はX線顕微鏡を含む、請求項9に記載の装置。
- 試料の画像を取得する画像取得部をさらに備えることを特徴とする請求項9に記載の装置。
- 画像取得部が、走査電子顕微鏡(SEM)、光学顕微鏡、又はその両方を含む、請求項11に記載の装置。
- 前記比較部による比較結果を出力する出力部をさらに備える又は前記出力部に接続されている、請求項9に記載の装置。
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Citations (3)
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JP2002296205A (ja) | 2001-03-29 | 2002-10-09 | Japan Tissue Engineering:Kk | 培養組織の元素組成分析方法、移植適性判定方法及び品質管理方法並びに、移植適性を有する培養組織の製造方法及び移植適性を有する培養組織 |
JP2010008406A (ja) | 2008-05-30 | 2010-01-14 | Shinichiro Isobe | 多光源顕微鏡 |
WO2014132383A1 (ja) | 2013-02-28 | 2014-09-04 | 一般社団法人ミネラル研究会 | 生体内元素検査方法 |
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JP2002296205A (ja) | 2001-03-29 | 2002-10-09 | Japan Tissue Engineering:Kk | 培養組織の元素組成分析方法、移植適性判定方法及び品質管理方法並びに、移植適性を有する培養組織の製造方法及び移植適性を有する培養組織 |
JP2010008406A (ja) | 2008-05-30 | 2010-01-14 | Shinichiro Isobe | 多光源顕微鏡 |
WO2014132383A1 (ja) | 2013-02-28 | 2014-09-04 | 一般社団法人ミネラル研究会 | 生体内元素検査方法 |
Non-Patent Citations (6)
Title |
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BANDURA, D. et al.,Characterization of phosphorus content of biological samples by ICP-DRC-MS: Potential tool for cance,Journal of Analytical Atomic Spectrometry,2004年,Vol.19, No.1,pp. 96-100,Abstract, Fig.4, Fig.5 |
CHMURA L. et al.,Quantification of selected elements in ovarian tumours and their potentials as a tissue classifier,J Physiol Pharmacol.,2017年10月,Vol.68, No.5,p.699-707,Abstract, Fig.6,7,10, Table.3 |
HARE, D. et al.,Elemental bio-imaging of melanoma in lymph node biopsies,The Analyst,2009年,Vol.134, No.3,pp.450-453,Abstract, Fig.1-4 |
PATAI, K. et al.,Phosphor/sulphur ratio: An indicator of malignant uterus change,Oncology Research,2005年,Vol.15, No.4,p.215-217,Abstract, Materials and Methods, p.216 light column, Fig.1 |
SIKORA, R. et al.,Measurement of BBN-induced alterations in rat urothelium by electron microscopic X-ray microanalysis,Urological Research,1994年,Vol. 22, No. 3,pp. 167-170,Abstract, Table.2, Fig.1-3 |
WANDZILAK A. et al.,X-ray fluorescence study of the concentration of selected trace and minor elements in human brain tu,Spectrochimica Acta Part B,2015年,Vol.114,p.52-57,Abstract, Fig.3 |
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