JP7091235B2 - Multi-layer culture vessel operation system, multi-layer culture vessel operation device, and multi-layer culture vessel operation method - Google Patents

Description

The present invention relates to a multi-layer culture container operation system for handling a multi-layer culture container containing a plurality of trays, a multi-layer culture container operation device, and a multi-layer culture container operation method.

In recent years, in order to culture a large amount of cells, a cell culture technique for culturing cells seeded in a culture medium using a multi-layer culture vessel containing a plurality of trays has been known. In such a cell culture technique, in order to reduce the burden on the operator, the culture medium in which the cells are seeded is introduced into the multi-layer culture container, or the multi-layer culture container is held and rotated in order to recover from the multi-layer culture container. A multi-layer culture vessel operating device that performs the handling operation of the above is used (see Patent Document 1).

JP 2015-505472

In general cell culture, since the cultured cells adhere to the wall surface of the multi-layer culture container, the culture solution is collected from the multi-layer culture container, then the trypsin solution is introduced into the multi-layer culture container, and the multi-layer culture container is shaken. By doing so, the cells are detached from the wall surface, and the tripsin solution is collected together with the detached cells. Conventionally, after introducing the trypsin solution into the multi-layer culture container, the multi-layer culture container is manually removed once from the multi-layer culture container operating device for peeling treatment, placed on a dedicated shaking device, and the multi-layer culture container is shaken. After that, in order to recover the trypsin solution, the work of mounting the multi-layer culture container on the multi-layer culture container operating device is performed again, which increases the burden on the operator and is required to improve the workability.

INDUSTRIAL APPLICABILITY According to the present invention, a series of operations in cell culture, particularly introduction of tripsin solution, cell detachment, and recovery of trypsin solution can be performed while the multi-layer culture container is mounted on the multi-layer culture container operating device. The present invention relates to a container operation system, a multi-layer culture container operation device, and a multi-layer culture container operation method.

The multi-layer culture container operation system according to the first aspect of the present invention can hold and rotate a movable trolley device equipped with a multi-layer culture container having a plurality of trays and the multi-layer culture container. A multi-layer culture vessel operating system comprising an operating device, wherein the operating device is rotatable about the first axis and a second axis substantially orthogonal to the first axis, and the multi-layer culture vessel can be rotated around the first axis. By rotating the multi-layer culture container around the second axis at the same time as rotating the multi-layer culture container around one axis, the reciprocating rotation is performed around the first axis and the second axis. It is possible to swing.
The multi-layer culture container operation system according to the second aspect of the present invention is capable of holding and rotating a movable trolley device equipped with a multi-layer culture container having a plurality of trays and the multi-layer culture container. A multi-layer culture vessel operating system comprising an operating device, wherein the operating device reciprocates to reciprocate the multilayer culture vessel along a first plane, a second plane, and a third plane that are substantially orthogonal to each other. It is possible to move, and by reciprocating the multi-layer culture vessel on the first axis, the multi-layer culture vessel is reciprocally rotated along the first plane, and the reciprocating swing is performed with the first axis. By reciprocating and reciprocating the multi-layer culture vessel on a second axis that is substantially orthogonal to each other, the reciprocating and reciprocating rotation of the multi-layer culture vessel along the second plane is performed .
In the multi-layer culture vessel operation system according to the first aspect, the operation device is different from the first operation unit that reciprocates along the first plane and the second plane, and the first operation unit. It can be configured to have a second operation unit that swings back and forth along the third plane.
In the multi-layer culture vessel operation system according to the second aspect, the first operation unit and the second operation unit can be configured to be arranged in the vertical direction.
In the multi-layer culture vessel operation system according to the second aspect, the second operation unit can be accommodated in the operation device main body, and when the reciprocating swing along the third plane is performed, the second operation unit is performed. The operation unit can be configured to protrude from the operation device main body.

In the multi-layer culture container operation system according to the third aspect of the present invention, a movable trolley equipped with a multi-layer culture container having a plurality of trays and the multi-layer culture container can be held and rotated by an arm. A multi-layer culture container operation system, wherein the trolley has an insertion hole through which the arm of the operation device is inserted, and the operation device holds the multi-layer culture container. A rotating portion that rotates the multilayer culture vessel on the first rotation axis and / or the second rotation axis, a shaking portion that shakes the multilayer culture vessel in the horizontal direction, and the rotating portion and the shaking. It has a control unit for controlling the operation of the unit, the arm is inserted into the insertion hole of the multi-layer culture container to hold the multi-layer culture container, and the control unit holds the multi-layer culture container with the arm. Then, the rotating operation is performed by the rotating portion, and the shaking portion is made to perform the shaking operation while the arm is inserted and held in the insertion hole.
In the multi-layer culture vessel operation system according to the third aspect, the shaking unit can be accommodated in the main body of the operating device, and the control unit operates the shaking unit when performing the shaking operation. It can be configured to protrude from the main body of the device.

The multi-layer culture container operating device according to the first aspect of the present invention has a support member that supports a multi-layer culture container having a plurality of trays having side surfaces so as to be movable up and down, and the multi-layer culture in cooperation with the support member. A locking member for fixing the container, a rotating portion for rotating the support member on the first rotation shaft and the second rotation shaft, and a control unit for controlling the operation of the support member and the rotation portion. The control unit reciprocates and reciprocates the multilayer culture vessel in the first direction and the second direction around the first rotation axis or the second rotation axis to the rotation unit to detach cells. The cell detachment function that executes the treatment, and when switching from the rotation motion in the first direction to the rotation motion in the second direction and from the rotation motion in the second direction to the first while performing the cell detachment function. It has a stop mode in which the movement of the multilayer culture vessel is stopped for a specified time when switching to the rotation operation in the direction.

The method for operating a multi-layer culture vessel according to the first aspect of the present invention includes a support member that supports a multi-layer culture container having a plurality of trays having side surfaces so as to be movable up and down, and the multi-layer culture in cooperation with the support member. A locking member for fixing the container, a rotating portion for rotating the support member on the first rotation shaft and the second rotation shaft, and a control unit for controlling the operation of the support member and the rotation portion. It is a method of operating a multi-layer incubator that operates the multi-layer culture container into which a tripsin solution has been introduced by using an operating device having the same. During the cell detachment treatment by reciprocating the multi-layer culture vessel in the first direction and the second direction, when switching from the rotation operation in the first direction to the rotation operation in the second direction and in the second direction. When switching from the rotation operation in the direction to the rotation operation in the first direction, the movement of the multilayer culture vessel is stopped for a specified time.

The multi-layer culture container operation system according to the fourth aspect of the present invention can hold and rotate a movable trolley device equipped with a multi-layer culture container having a plurality of trays and the multi-layer culture container. A multi-layer culture container operation system having an operating device, wherein the trolley device is detachably mounted on a trolley having wheels and the trolley, and fixes the multi-layer culture container to the trolley. The operating device has a fixing member, and the operating device holds the multi-layer culture container together with the fixing member, and rotates the multi-layer culture container on a first rotation axis and / or a second rotation axis. A shaking unit that holds the multi-layer culture container together with the fixing member and shakes the multi-layer culture container in the horizontal direction, and a control unit that controls the operation of the rotating unit and the shaking unit. Then, the control unit causes the shaking unit to perform the shaking operation following the rotation operation by the rotating unit without returning the multilayer culture container to the trolley.
In the multi-layer culture vessel operation system according to the fourth aspect, the control unit may reciprocate and reciprocate the multi-layer culture vessel around the first rotation axis or the second rotation axis to the rotation unit. It can be configured to be possible.
In the multi-layer culture vessel operation system according to the fourth aspect, the shaking unit can be accommodated in the operating device main body, and the control unit can use the shaking unit when performing the shaking operation. It can be configured to protrude from the main body of the operating device.
In the multi-layer culture container operation system according to the fourth aspect, the control unit causes the shaking unit to directly receive the multi-layer culture container together with the fixing member from the rotating unit when the shaking operation is performed. Can be configured.
In the multi-layer culture vessel operation system according to the fourth aspect, the rotating portion and the shaking portion include a power motor and can be configured to be driven by electric power.

The multi-layer culture container operating device according to the second aspect of the present invention receives the multi-layer culture container from a movable trolley equipped with a multi-layer culture container containing a plurality of trays, holds the multi-layer culture container, and rotates. A rotating unit that holds the multi-layered culture container and rotates the multi-layered culture container on the first rotation axis and / or the second rotation axis, and a rotating portion that is capable of rotating the multi-layer culture container. A shaking part that is arranged below the rotating part, is housed inside the main body when not in use, protrudes to the outside of the main body when in use, holds the multilayer culture container, and shakes the culture container in the horizontal direction. It has a rotating unit and a control unit that controls the operation of the shaking unit, and the control unit has the shaking operation following the rotation operation by the rotating unit without returning the multilayer culture container to the trolley. Have the unit perform the shaking operation.
In the multi-layer culture container operating device according to the second aspect, the control unit causes the shaking unit to directly receive the multi-layer culture container together with the fixing member from the rotating unit when the shaking operation is performed. Can be configured.
The multi-layer culture vessel operating device according to the third aspect of the present invention has a support member that supports a multi-layer culture vessel containing a plurality of trays so as to be movable up and down, and a support member that cooperates with the support member to fix the multi-layer culture vessel. It has a locking member, a rotating portion that rotates the support member on the first rotation shaft and the second rotation shaft, and a control unit that controls the operation of the support member and the rotation portion. The control unit reciprocates the multilayer culture vessel in the first direction and the second direction around the first rotation axis or the second rotation axis to execute the cell detachment treatment on the rotation unit. When switching from the rotation motion in the first direction to the rotation motion in the second direction and from the rotation motion in the second direction to the first direction while performing the cell detachment function and the cell detachment function. It has a stop mode in which the movement of the multilayer culture vessel is stopped for a specified time when switching to the rotation operation.
In the multilayer culture vessel operating device according to the third aspect, the tray has a first side surface, a second side surface adjacent to the first side surface, and a third side surface adjacent to the second side surface. The control unit is provided with a third side surface and an adjacent fourth side surface, and the control unit rotates so that the trypsin solution collides with the first side surface, the second side surface, the third side surface, and the fourth side surface in this order. It can be configured to have a side-attached cell recovery function for manipulating the portion.
In the multi-layer culture vessel operating device according to the second or third aspect, the rotating portion includes a power motor and can be configured to be driven by electric power.

The method for operating a multi-layer culture vessel according to a second aspect of the present invention includes a rotating portion that holds a multi-layer culture vessel containing a plurality of trays and rotates the multi-layer culture vessel, and a rotating portion below the rotating portion. An operating device having a shaking unit that is arranged, is housed inside the main body when not in use, protrudes to the outside of the main body, holds the multi-layer culture container, and shakes the multi-layer culture container in the horizontal direction. It is a method of operating a multi-layer incubator for operating the multi-layer culture container, wherein the shaking operation is performed on the shaking unit following the rotation operation by the rotating unit.
In the method for operating a multi-layer culture vessel according to a third aspect of the present invention, a support member that supports a multi-layer culture vessel containing a plurality of trays so as to be movable up and down, and the multi-layer culture vessel are fixed in cooperation with the support member. An operating device having a locking member, a rotating portion that rotates the support member on the first rotating shaft and the second rotating shaft, and a control unit that controls the operation of the support member and the rotating portion. It is a method of operating a multi-layer incubator for operating the multi-layer culture vessel into which a tripsin solution is introduced, in a first direction and in the rotating portion about the first rotation axis or the second rotation axis. During the cell detachment process by reciprocating the multilayer culture vessel in the second direction, when switching from the rotation operation in the first direction to the rotation operation in the second direction and rotation in the second direction. When switching from the operation to the rotation operation in the first direction, the movement of the multi-layer culture vessel is stopped for a specified time.
In the method for operating a multi-layer culture vessel according to the third aspect, the tray has a first side surface, a second side surface adjacent to the first side surface, and a third side surface adjacent to the second side surface. A third side surface and an adjacent fourth side surface are provided so that the trypsin solution collides with the first side surface, the second side surface, the third side surface, and the fourth side surface in this order after the cell detachment treatment. It can be configured to operate the rotating portion.

According to the present invention, a series of processes of introduction of trypsin solution, cell detachment, and recovery of trypsin solution can be performed while the multi-layer culture container is mounted on the multi-layer culture container operating device, so that workability in cell culture can be improved. Can be improved.

It is a figure for demonstrating the multilayer culture container which concerns on 1st Embodiment. It is a figure for demonstrating the method of distributing a liquid to each tray in a multi-layer culture vessel. It is a perspective view which shows the multilayer culture vessel operation system which concerns on 1st Embodiment. It is a perspective view for demonstrating the carriage apparatus which concerns on 1st Embodiment. It is a block diagram for demonstrating the multilayer culture vessel operation apparatus which concerns on 1st Embodiment. It is a figure for demonstrating the relationship between the locked part of a fixing member, and the locking member of a multilayer culture vessel operation apparatus. It is a perspective view which shows the state which the multi-layer culture vessel is temporarily fixed to an arm. It is a figure which shows an example of the rotation operation of the rotation part about the rotation axis X1. It is a figure which shows an example of the rotation operation of the rotation part about the rotation axis X2, and the rotation operation of the rotation part about rotation axes X1 and X2. It is a perspective view which shows the state which the shaking part was projected in the multi-layer culture vessel operation apparatus. It is a figure for demonstrating the relationship between an arm and an insertion hole in a shaking motion. It is a flowchart which shows the cell culture process which concerns on 1st Embodiment. It is a flowchart which shows the culture solution introduction process of step S1. It is a flowchart which shows the culture solution recovery process of a step S3. It is a flowchart which shows the trypsin solution introduction process of step S4. It is a flowchart which shows the cell detachment process of step S5. It is a flowchart which shows the trypsin liquid recovery process of a step S6. It is a block diagram for demonstrating the multilayer culture vessel operation apparatus which concerns on 2nd Embodiment.

[First Embodiment]
The multi-layer culture vessel operation system 1 according to the first embodiment will be described below. The multi-layer culture container operation system 1 according to the present embodiment is a system for operating (handling) the multi-layer culture container 30 used for cell culture and the like. Therefore, first, the multilayer culture vessel 30 according to the present embodiment will be described. FIG. 1 is a diagram for explaining the multilayer culture vessel 30 according to the present embodiment, and is a cross-sectional view showing the multilayer culture vessel 30. As shown in FIG. 1, the multi-layer culture vessel 30 has a configuration in which a plurality of trays 31 are stacked in order to culture a large amount of cells. When culturing cells in the multi-layer culture container 30, for example, as shown in FIG. 2A, the multi-layer culture container 30 is tilted by about 90 ° so that the vent cap 32 is on the lower side. Then, the vent cap 32 and the pump are connected, and the culture solution in which the cells are seeded is introduced into the multi-layer culture vessel 30 by the pump. Next, as shown in FIG. 2B, when the multi-layer culture container 30 is returned upright, the culture solution is distributed to each tray 31 of the multi-layer culture container 30, and cell culture is performed in each tray 31.

In addition, after cell culture, a culture solution recovery process for collecting the culture solution from the multi-layer culture container 30, and introduction of the trypsin solution for introducing the trypsin solution into the multi-layer culture container 30 in order to exfoliate the cells adhering to the wall surface of the multi-layer culture container 30. The treatment and the trypsin solution recovery treatment for recovering the trypsin solution containing the detached cells from the multi-layer culture vessel 30 are performed. Also in these treatments, it is necessary to introduce or recover the culture solution or trypsin solution by tilting the multi-layer culture container 30 or the like. However, when the operator manually operates the multi-layer culture container 30, the multi-layer culture container 30 containing the culture solution and the trypsin solution is heavy, and the burden on the operator increases. In addition, there are cases where the work varies due to manual work, or when the operator unnecessarily touches the multi-layer culture container 30, the multi-layer culture container is damaged and contamination occurs. Therefore, there is a need for a system for operating the multi-layer culture container 30, such as the multi-layer culture container operation system 1 according to the present embodiment.

FIG. 3 is a perspective view showing the multilayer culture vessel operation system 1 according to the present embodiment. As shown in FIG. 3, the multi-layer culture container operation system 1 according to the present embodiment includes a multi-layer culture container operation device 10 and a bogie device 20. Each configuration will be described below.

FIG. 4 is a perspective view for explaining the dolly device 20 according to the present embodiment. As shown in FIG. 4A, the trolley device 20 includes a trolley 21 having wheels 22 and a fixing member 23 for fixing the multilayer culture container 30 to the trolley 21. Further, in the carriage device 20, the carriage 21 and the fixing member 23 are detachable. Specifically, by lifting the fixing member 23 upward (Z-axis positive direction) with respect to the trolley 21, the fixing member 23 can be removed from the trolley 21 as shown in FIG. 4 (B). On the contrary, by placing the fixing member 23 on the trolley 21, the fixing member 23 can be attached to the trolley 21. The trolley 21 and the fixing member 23 each have a fitting portion (not shown) that fits each other, whereby the trolley 21 and the fixing member 23 can be attached and detached only in the vertical direction (Z-axis direction). It is fixed in the horizontal direction (XY axis direction). As a result, it is possible to prevent the fixing member 23 from falling from the carriage 21 while the carriage device 20 is moving.

As shown in FIG. 4, the fixing member 23 can fix the plurality of multi-layer culture containers 30 at the same time in a state where the plurality of multi-layer culture containers 30 are arranged in parallel. In the present embodiment, the bogie device 20 is configured so that a maximum of four multi-layer culture containers 30 can be mounted, but the present invention is not limited to this configuration, and a maximum of 1 to 3 multi-layer culture containers 30 can be mounted. The trolley device 20 may be configured in the trolley device 20, or the trolley device 20 may be configured so that five or more multilayer culture containers 30 can be mounted on the trolley device 20.

As shown in FIG. 4, the fixing member 23 has four lengths of a pedestal 24 on which the multilayer culture vessel 30 is placed and a multilayer culture vessel 30 so that the multilayer culture vessel 30 does not shift in the horizontal direction (XY axis direction). A frame member 25 that guards the sides, and a fastening member 26 that is locked to the frame member 25 and guards the multilayer culture vessel 30 so that it does not pop out from the upward direction (Z-axis direction) when the multilayer culture vessel 30 is rotated. And have. For example, the worker places the multilayer culture vessel 30 on the pedestal 24 and in the frame of the frame member 25, and then presses the upper side of the multilayer culture vessel 30 with the fastening member 26 so that the fastening member 26 is pressed. By locking the frame member 25 and the frame member 25, the multilayer culture vessel 30 can be fixed to the fixing member 23.

Further, as will be described later, the fixing member 23 has a locked portion 28 that locks with the locking member 111 of the multilayer culture vessel operating device 10. In the present embodiment, the locked portion 28 is continuously formed in a portion of the frame member 25 where the side surface of the multilayer culture vessel 30 extends in the arrangement direction of the multilayer culture vessel 30, and as shown in FIG. It is a thin plate-shaped member having a length W1.

Next, the multilayer culture vessel operating device 10 according to the present embodiment will be described. FIG. 5 is a block diagram showing a multilayer culture vessel operating device 10 according to the present embodiment. As shown in FIG. 5, the multilayer culture vessel operating device 10 includes a rotating unit 11, a rotating drive unit 12, a shaking unit 13, a shaking drive unit 14, a drive control unit 15, an operation unit 16, and an arm drive. A portion 17, a pair of arms 18, and a main body 19 are provided. The drive control unit 15 introduces a culture solution introduction process for introducing a culture solution in which cells are seeded into the multi-layer culture container 30, a culture solution recovery process for recovering the culture solution from the multi-layer culture container 30, and a trypsin solution into the multi-layer culture container 30. An operation program for operating the multi-layer culture container 30, such as a tripsin introduction process, a cell exfoliation process for shaking the multi-layer culture container 30, and a tripsin solution recovery process for recovering the trypsin solution from the multi-layer culture container 30, is stored in advance. In the present embodiment, the drive control unit 15 controls the operations of the rotation drive unit 12 and the shaking drive unit 14 based on the operation program, so that the rotation drive unit 12 rotates the rotation unit 11 and shakes. By controlling the operation of the drive unit 14, the shaking unit 14 is made to shake the shaking unit 13. In the present embodiment, the rotation drive unit 12 and the shaking drive unit 14 have an electric power motor, and receive power to drive the rotation unit 11 and the shaking unit 13.

As shown in FIGS. 3 and 5, the rotating portion 11 has a pair of locking members 111 that function as holding members for the multilayer culture vessel 30. The locking member 111 is fixed to both side surfaces of the rotating portion 11 and includes recesses 112 as shown in FIGS. 5 and 6A to 6C. Further, the recess 112 has a tapered portion 113 and a groove portion 114, and as will be described later, when the fixing member 23 is moved upward by the arm 18, the fixing member 23 is as shown in FIG. 6 (B). It can be locked to the locked portion 28 of the above, and can be fixed to the rotating portion 11 by sandwiching the fixing member 23 together with the arm 18. 6 (A) and 6 (B) are views for explaining the relationship between the locked portion 28 and the locking member 111, and FIG. 6 (C) is an enlarged view of the locking member 111. ..

Further, the multi-layer culture container operating device 10 includes a pair of arms 18 that function as support members for the multi-layer culture container 30. As shown in FIG. 7, the pair of arms 18 can be inserted into the two insertion holes 27 provided in the fixing member 23, respectively. FIG. 7 is a perspective view showing a state in which the multilayer culture vessel 30 is temporarily fixed to the arm 18. The pair of arms 18 can be moved in the vertical direction (Z-axis direction) by the arm drive unit 17, and the arm drive unit 17 has two arms 18 fixed members 23 based on the instructions of the drive control unit 15. The arm 18 is driven in the Z-axis direction to a height position at which the arm 18 can be inserted into the insertion hole 27. As a result, the operator can move the bogie device 20 toward the main body 19 and insert the two arms 18 into the two insertion holes 27 of the fixing member 23. Further, a clamp 181 is housed on the side surface of the tip end portion of the arm 18, and when the arm 18 inserts the insertion hole 27, the clamp 181 projects from the side surface of the tip end portion of the arm 18 that has passed through the insertion hole 27. Then, the drive control unit 15 drives the arm 18 upward (Z-axis positive direction) by the arm drive unit 17 in a state where the two arms 18 are inserted into the two insertion holes 27 of the fixing member 23. , The multilayer culture vessel 30 is lifted to the position of the rotating portion 11. As a result, as shown in FIG. 6B, the locking member 111 of the rotating portion 11 and the locked portion 28 of the fixing member 23 are locked, and the fixing member 23 is a pair of locking members 111 and a pair. By being sandwiched by the rotating portion 11 by the arm 18, the multilayer culture vessel 30 is fixed to the rotating portion 11 together with the fixing member 23. In this embodiment, the arm drive unit 17 can drive a pair of arms 18 by a power motor or an air cylinder.

Then, as shown by reference numerals R and P in FIG. 3, the drive control unit 15 causes the rotation drive unit 12 to perform a rotation operation of rotating the rotation unit 11 around the two axes of the rotation axes X1 and X2. As shown in FIG. 3, the rotation axis X1 is a rotation axis extending in the X-axis direction, whereby the multi-layer culture vessel 30 held by the rotation portion 11 and the rotation portion 11 can be rotated in the roll direction R. can. Further, the rotation axis X2 is a rotation axis extending in the Y-axis direction, whereby the multilayer culture vessel 30 held by the rotation portion 11 and the rotation portion 11 can be rotated in the pitch direction P. In the rotation operation, the rotation in the roll direction R can be performed in the range of less than ± 180 °, and in the present embodiment, the rotating portion 11 can be rotated in the range of ± 120 ° in the roll direction R. Further, the rotation in the pitch direction P can also be performed in a range of less than ± 180 °, and in the present embodiment, the rotating portion 11 can be rotated in the roll direction R within a range of ± 30 °. In the present embodiment, the rotation drive unit 12 includes a power motor and / or an air cylinder that rotates the rotation unit 11 on the rotation shaft X1, and a power motor and / or an air cylinder that rotates the rotation unit 11 on the rotation shaft X2. The rotating portion 11 can be rotated by two axes.

Here, FIG. 8 is a diagram showing an example of the rotational operation of the rotating portion 11 (multilayer culture vessel 30) about the rotation axis X1, and in FIG. 8A, the rotating portion 11 holds the multilayer culture vessel 30. It shows the lifted state (reference position). In the present embodiment, the rotation drive unit 12 moves the rotation unit 11 (multilayer culture vessel 30) from the reference position shown in FIG. 8 (A) with the rotation axis X1 as the center, for example, as shown in FIG. 8 (B). It can be rotated 90 ° to the left. Further, the rotation drive unit 12 can rotate the rotation unit 11 (multilayer culture vessel 30) by 100 ° to the left from the reference position about the rotation axis X1 as shown in FIG. 8C. As shown in FIG. 8D, the rotation axis X1 can be rotated 120 ° to the left from the reference position. Further, the rotation drive unit 12 can also rotate the rotation unit 11 (multilayer culture vessel 30) in the range of 0 to 120 ° to the right from the reference position. In this way, the rotation drive unit 12 can rotate the rotation unit 11 (multilayer culture vessel 30) in the roll direction R in the range of ± 0 to 120 ° from the reference position about the rotation axis X1.

Further, FIG. 9 shows a rotation operation of the rotating portion 11 (multilayer culture container 30) about the rotation axis X2 and a rotation operation of the rotation unit 11 (multilayer culture container 30) on the two axes of the rotation axes X1 and X2. It is a figure which shows an example. The rotation drive unit 12 can rotate the rotation unit 11 (multilayer culture vessel 30) in the pitch direction P about the rotation axis X2 from the reference position shown in FIG. 9A. For example, in the example shown in FIG. 9B, the rotating portion 11 (multilayer culture vessel 30) is rotated on the axis X2 so that the upper portion of the rotating portion 11 (multilayer culture vessel 30) is tilted forward (in the negative direction on the X axis). It is rotated 20 ° as the center. Further, the rotation drive unit 12 rotates the rotation unit 11 (multilayer culture container 30) about the rotation axis X2 so that the lower part of the rotation unit 11 (multilayer culture container 30) tilts forward (X-axis negative direction). You can also do it. In this way, the rotation drive unit 12 can rotate the rotation unit 11 (multilayer culture vessel 30) in the pitch direction P in the range of ± 0 to 30 ° from the reference position about the rotation axis X2.

Further, as shown in FIGS. 9C and 9D, the rotation drive unit 12 rotates the rotation unit 11 (multilayer culture vessel 30) in the roll direction R about the rotation axis X1 and rotates the rotation axis X2. Can be rotated in the pitch direction P about the center. For example, in the figure shown in FIG. 9C, the rotating portion 11 (multilayer culture vessel 30) is rotated 100 ° to the left about the rotation axis X1 and the upper part of the rotating portion 11 (multilayer culture vessel 30) is above. It is rotated by 20 ° around the rotation axis X2 so as to be tilted forward (in the negative direction of the X axis). Further, in the example shown in FIG. 9D, the rotating portion 11 (multilayer culture vessel 30) is rotated by 120 ° to the left about the rotation axis X1, and the upper part of the rotating portion 11 (multilayer culture vessel 30) is above. It is rotated by 20 ° around the rotation axis X2 so as to be tilted forward (in the negative direction of the X axis).

Further, the rotation drive unit 12 can also perform a swing operation in which the rotation unit 11 (multilayer culture vessel 30) is reciprocally rotated about the rotation axis X1 or the rotation axis X2. For example, the rotation drive unit 12 reciprocates the rotation unit 11 (multilayer culture vessel 30) in the roll direction R within a range of ± 120 ° about the rotation axis X1, so that the rotation drive unit 12 swings around the rotation axis X1. It can be performed. Further, the rotation drive unit 12 rotates after tilting the rotation unit 11 (multilayer culture container 30) forward (X-axis negative direction) above the rotation unit 11 (multilayer culture container 30) with the rotation axis X2 as the center. By reciprocating in the pitch direction P within a range of ± 20 ° so that the lower part of the portion 11 (multilayer culture vessel 30) is tilted forward (in the negative direction of the X axis), a swinging motion centered on the rotation axis X2 is performed. You can also do it.

The shaking unit 13 is housed inside the main body 19 of the multilayer culture vessel operating device 10 when the shaking operation described later is not performed, and when the shaking operation is performed, the drive control unit 13 is as shown in FIG. Based on the control of 15, the shaking drive unit 14 projects to the outside of the main body 19. Note that FIG. 10 is a perspective view showing a state in which the shaking portion 13 is projected in the multilayer culture vessel operating device 10. The shaking portion 13 has an upper surface 131 having a size on which the multi-layer culture container 30 can be placed together with the fixing member 23, and the multi-layer culture container 30 together with the fixing member 23 is placed on the upper surface 131 to perform a horizontal shaking operation. be able to. Further, the shaking portion 13 has a fitting portion (not shown) for mating with the fixing member 23, similarly to the bogie 21, thereby, in the vertical direction (Z-axis direction) with the fixing member 23. Although it is removable, it limits the movement of the fixing member 23 in the horizontal direction (XY axis direction) and prevents the fixing member 23 from falling from the shaking portion 13. The shaking drive unit 14 can cause the shaking unit 13 to perform any shaking operation as long as it is a shaking operation in the horizontal direction (XY axis direction). For example, the shaking drive unit 14 has a left-right shaking motion (reciprocating motion in the Y-axis direction), a front-back shaking motion (reciprocating motion in the X-axis direction), or a shaking motion in the figure eight direction (X-axis direction). And various shaking operations such as a combination of the Y-axis direction) can be performed by the shaking unit 13.

In the present embodiment, the drive control unit 15 can cause the shaking unit 13 to perform the shaking operation following the rotation operation by the rotating unit 11. In this case, the drive control unit 15 controls the arm drive unit 17 to drive the pair of arms 18 downward (Z-axis negative direction) when the rotation operation by the rotation unit 11 is completed, and the multi-layer culture container together with the fixing member 23. 30 is operated so as to be placed on the upper surface 131 of the shaking portion 13. Then, the drive control unit 15 controls the shaking drive unit 14 to cause the shaking unit 13 to perform a shaking operation of shaking the multilayer culture vessel 30. In the present embodiment, the arm 18 is not housed in the main body 19 and the shaking operation is performed while the arm 18 is projected. In the present embodiment, as shown in FIG. 11, since the inner width W3 of the insertion hole 27 is designed to be wider than the total of the outer width W2 of the arm 18 and the shaking width of the shaking portion 13, the arm 18 is inserted. The shaking operation can be performed in the state of being inserted into the hole 27. The clamp 181 is housed in the arm 18 during the shaking operation. Further, FIG. 11 is a diagram for explaining the relationship between the arm 18 and the insertion hole 27 in the shaking operation.

Further, the drive control unit 15 can cause the rotating unit 11 to perform the rotational operation following the shaking operation by the shaking unit 13. In this case, when the shaking operation by the shaking unit 13 is completed, the drive control unit 15 controls the arm drive unit 17 to drive the pair of arms 18 upward (Z-axis positive direction), and the multi-layer culture container together with the fixing member 23. The 30 is operated to lift up. Then, the drive control unit 15 controls the shaking drive unit 14 to accommodate the shaking unit 13 inside the main body 19, and then controls the rotation drive unit 12 to cause the rotation unit 11 to perform a rotation operation.

The operation unit 16 is a device for an operator to input an instruction, and may be configured to include, for example, a touch panel. By operating the operation unit 16, the operator transmits instructions such as start, pause, and end of the operation program stored in advance by the drive control unit 15 to the drive control unit 15, and the rotating unit 11 and the shaking unit 11 and the shaking unit. The operation of 13 can be started, paused, ended, and the like. Further, by operating the operation unit 16, the operator can store a new program in the drive control unit 15 or change a part of the stored operation program. For example, by operating the operation unit 16, the operator changes the inclination angle of the rotation axis X1 of the rotation unit 11 from 100 ° to 120 ° in the culture solution recovery process for collecting the culture solution from the multilayer culture container 30. You can do things like that.

Next, the cell culture treatment in the multi-layer culture container operation system 1 according to the present embodiment will be described. FIG. 12 is a flowchart showing a cell culture process in the multi-layer culture container operation system 1 according to the present embodiment. Each process performed by the multilayer culture vessel operating device 10 is started by the operator pressing the process start button of the operation unit 16, and when the process is completed, the operation of the multilayer culture vessel operating device 10 is terminated. .. Therefore, when operating the multilayer culture vessel operating device 10, the operator presses the processing start button of the operation unit 16 for each processing to proceed with the processing. The operation of the multilayer culture vessel operating device 10 in each process is stored in advance in the operation program of the drive control unit 15, and the operator simply presses the same process start button, and the drive control unit 15 performs each process. The corresponding operation is instructed to the rotation drive unit 12 or the shaking drive unit 14, and the rotation unit 11 or the shaking unit 13 is operated.

In step S1, a culture medium introduction process is performed in which the culture medium in which the cells are seeded is introduced into the multi-layer culture vessel 30. Here, FIG. 13 is a flowchart showing the culture solution introduction process of step S1. First, in step S101, the trolley device 20 equipped with the empty multi-layer culture container 30 is set at the fixed position of the multi-layer culture container operation device 10 by the operator. The fixed position means that the pair of arms 18 are at the same height as the insertion hole 27 of the trolley device 20, and when the trolley device 20 is set at the fixed position, the two arms 18 are the fixing member 23. It is a position where two insertion holes 27 are inserted and the tip end portion of the arm 18 protrudes from the insertion holes 27. Then, when the operator operates the operation unit 16, the process of step S102 is performed.

In step S102, the clamp 181 is projected from the tip end portion of the arm 18 by the rotation drive unit 12, and the multilayer culture vessel 30 fixed by the fixing member 23 is temporarily fixed to the arm 18 together with the fixing member 23. Further, in step S103, the arm driving unit 17 drives the pair of arms 18 fixing the multilayer culture vessel 30 upward (Z-axis positive direction). As a result, the fixing member 23 is removed from the trolley 21 of the trolley device 20, and the multi-layer culture container 30 together with the fixing member 23 is lifted upward (Z-axis positive direction) until it comes into contact with the locking member 111 of the rotating portion 11 by the arm 18. Be done. As a result, the fixing member 23 is sandwiched between the locking member 111 and the arm 18, and the multilayer culture vessel 30 is held together with the fixing member 23 by the rotating portion 11.

In step S104, the dolly 21 is removed from the fixed position by the operator. Then, when the operator operates the operation unit 16, the process of step S105 is performed. In step S105, the rotation drive unit 12 performs a process of rotating the rotation unit 11. For example, as shown in FIG. 8B, the drive control unit 15 controls the rotation unit 11 so that the rotation unit 11 rotates 90 ° to the left from the reference position about the rotation axis X1. The multi-layer culture vessel 30 can be rotated 90 ° to the left.

In step S106, the worker introduces the culture medium in which the cells are seeded into the multi-layer culture vessel 30. For example, the operator can open the vent cap 32 of the multilayer culture vessel 30, connect a pump (not shown) to the vent cap 32, and use the pump to introduce the culture solution into the multilayer culture vessel 30. As a result, as shown in FIG. 2A, the culture solution is collected under the multilayer culture vessel 30 rotated by 90 °. When the introduction of the culture solution is completed, the vent cap 32 is closed by the operator. Then, when the operator operates the operation unit 16, the process of step S107 is performed.

In step S107, the rotation driving unit 12 returns the multilayer culture vessel 30 to the reference position as shown in FIG. 9A. As a result, as shown in FIG. 2B, the culture solution is distributed to each tray 31 of the multilayer culture container 30. In the following step S108, the dolly 21 is set at a fixed position by the operator. Then, when the operator operates the operation unit 16, the process of step S109 is performed. In step S109, the pair of arms 18 are driven downward (Z-axis negative direction) by the arm driving unit 17, and the fixing member 23 fixing the multilayer culture vessel 30 is mounted on the carriage 21. As a result, the operator can move the multi-layer culture container 30 to which the culture solution is distributed to the culture chamber or the incubator by the trolley device 20.

Then, returning to FIG. 12, in step S2, cell culture is performed for a certain period of time in the culture room or the incubator. Then, in step S3, a culture solution recovery process for recovering the culture solution after cell culture from the multilayer culture vessel 30 is performed. FIG. 14 is a flowchart showing the culture solution recovery process in step S3.

As shown in FIG. 14, in steps S301 to S305, the bogie device 20 is set at a fixed position (step S301), and the multilayer culture vessel 30 together with the fixing member 23 is temporarily fixed to the arm 18 as in steps S101 to S105. (Step S302), the multilayer culture vessel 30 is lifted upward and held by the rotating portion 11 by being sandwiched between the locking member 111 and the arm 18 (step S303), and the bogie 21 is removed from the fixed position by the operator. (Step S304), the rotation operation of the multilayer culture vessel 30 is performed (step S305).

In step S305, as shown in FIGS. 9C and 9D, the multilayer culture vessel 30 is rotated in the roll direction R about the rotation axis X1 and the pitch direction P about the rotation axis X2. May be rotated to. Further, the draining process may be performed before the rotation operation is performed in step S305. The draining process is a process of rotating the rotating portion 11 so as to remove droplets adhering to the side surface of each tray 31 of the multilayer culture vessel 30. Specifically, as shown in FIG. 8B, the drive control unit 15 rotates the multilayer culture vessel 30 by 90 ° in the roll direction R, and rotates the multilayer culture vessel 30 by + 30 ° in the pitch direction P. Rotate (tilt 30 ° in the pitch direction P so that the upper side tilts forward), and then rotate the multilayer culture vessel 30 by -30 ° in the pitch direction P (30 ° in the pitch direction P so that the upper side tilts backward). Tilt). Thereby, the droplets scattered on the side surface of the tray 31 of the multilayer culture vessel 30 can be removed by the culture solution.

Then, in step S306, the operator performs the work of collecting the culture broth from the multilayer culture vessel 30. For example, the operator can recover the culture solution from the multi-layer culture container 30 by connecting the pump to the vent cap 32 of the multi-layer culture container 30, opening the vent cap 32, and sucking the culture solution by the pump.

Next, returning to FIG. 12, in step S4, a trypsin solution introduction process is performed in which the trypsin solution is introduced into the multilayer culture vessel 30 in order to exfoliate the cultured cells adhering to the wall surface of each tray 31 of the multilayer culture vessel 30. FIG. 15 is a flowchart showing the trypsin solution introduction process in step S4.

First, in step S401, the rotation of the multilayer culture vessel 30 is performed in the same manner as in step S105. For example, as shown in FIG. 9B, the multilayer culture vessel 30 can be rotated by 90 °. Then, in step S402, the trypsin solution is introduced into the multilayer culture vessel 30 by the operator. For example, as in step S106, the operator can open the vent cap 32 of the multilayer culture vessel 30, connect the pump to the vent cap 32, and use the pump to introduce the trypsin solution into the multilayer culture vessel 30. As a result, as shown in FIG. 2A, the trypsin solution is collected under the multilayer culture vessel 30 rotated by 90 °. When the introduction of the trypsin solution is completed, the vent cap 32 is closed by the operator. Then, in step S403, similarly to step S107, the multilayer culture vessel 30 is returned to the reference position as shown in FIG. 9A by the rotation driving unit 12. As a result, as shown in FIG. 2B, the trypsin solution is distributed to each tray 31 of the multilayer culture vessel 30.

Then, returning to FIG. 12, in step S5, a cell exfoliation process for exfoliating the cultured cells adhering to the wall surface of each tray 31 of the multilayer culture vessel 30 is performed. FIG. 16 is a flowchart showing the cell detachment process of step S5. In the present embodiment, the process of step S501 is started by the operator operating the operation unit 16.

In step S501, the shaking portion 13 housed inside the multilayer culture vessel operating device 10 is projected to the outside of the main body 19. Then, in step S502, the pair of arms 18 are driven downward (Z-axis negative direction) by the arm driving unit 17, and the multilayer culture vessel 30 held by the rotating unit 11 is placed on the upper surface 131 of the shaking unit 13 together with the fixing member 23. Be done.

In step S503, the shaking driving unit 14 performs the shaking operation of the shaking unit 13. The shaking driving unit 14 shakes the multi-layer culture vessel 30 by shaking the shaking unit 13 in the left-right direction (Y-axis direction), the front-back direction (X-axis direction), or the figure eight direction (XY-axis direction). .. As a result, the cultured cells attached to the wall surface of each tray 31 are peeled off. In step S504, similarly to step S103, the multilayer culture vessel 30 is lifted up by the pair of arms 18 and held by the rotating portion 11. Then, in step S505, the shaking unit 13 is housed inside the multilayer culture vessel operating device 10 by the shaking driving unit 14.

Then, returning to FIG. 12, in step S6, a trypsin solution recovery process for recovering the trypsin solution containing the detached cultured cells is performed. FIG. 17 is a flowchart showing the trypsin solution recovery process in step S6.

Specifically, in step S601, the rotation operation of the multilayer culture vessel 30 is performed in the same manner as in step S305. For example, as shown in FIGS. 9C and 9D, the multilayer culture vessel 30 may be rotated in the roll direction R about the rotation axis X1 and in the pitch direction P around the rotation axis X2. good. Further, in step S601, as in step S305, the draining process may be performed before the rotation operation. Then, in step S602, similarly to step S306, the operator performs the work of recovering the trypsin solution from the multilayer culture vessel 30 by using a pump or the like. Then, in step S603, the rotation driving unit 12 performs a process of returning the multilayer culture vessel 30 to the reference position.

As described above, the multi-layer culture container operating device 10 according to the present embodiment integrally includes a rotating unit 11 that rotates the multi-layer culture container 30 and a shaking unit 13 that shakes the multi-layer culture container 30. Without returning the multilayer culture vessel 30 to the trolley 21, the shaking unit 13 can be made to perform the shaking operation following the rotation operation by the rotating unit 11. As a result, in the multi-layer culture container operating device 10 according to the present embodiment, the multi-layer culture container 30 is held by the multi-layer culture container operating device 10, and the culture solution recovery process, tripsin solution introduction process, cell detachment process, and so on. A series of treatments up to the recovery treatment of the trypsin solution containing the detached cultured cells can be performed without the operator touching the multilayer culture vessel 30.

In particular, conventionally, after introducing the trypsin solution into the multilayer culture vessel 30, the operator manually removes the multilayer culture vessel 30 from the multilayer culture vessel operating device, replaces it with a dedicated shaker, and uses a dedicated shaker. After shaking the multi-layer culture container 30, the operator manually placed the multi-layer culture container 30 on the multi-layer culture container operating device again to recover the trypsin solution. Therefore, there is a problem that the labor of the worker in the cell culture process increases. In addition, when the operator manually performs the work, the work may vary, or when the operator touches the multilayer culture vessel 30 unnecessarily, the multilayer culture vessel 30 may be damaged and contaminate the medium. In some cases. On the other hand, in the present embodiment, the above-mentioned series of treatments can be performed without the operator touching the multilayer culture vessel 30, so that the above-mentioned problems can be solved.

Further, in the present embodiment, the rotation drive unit 12 and the shaking drive unit 14 have a power motor and operate by receiving power supply. Conventionally, a multi-layer culture container operating device having a rotary drive unit has been known, but such a multi-layer culture container operating device uses hydraulic drive. However, hydraulic drive may contaminate the clean room, so it may not be usable in some clean rooms. On the other hand, in the multilayer culture vessel operating device 10 according to the present embodiment, a power motor is used for the rotation drive unit 12 and the shaking drive unit 14, and the device can be used regardless of the clean room.

[Second Embodiment]
The multi-layer culture container operating device 10a according to the second embodiment is mainly different from the multi-layer culture container operating device 10 according to the first embodiment in that the shaking unit 13 and the shaking driving unit 14 are not provided. Hereinafter, the same configurations as those of the first embodiment are designated by the same reference numerals, and the description thereof will be omitted.
FIG. 18 is a block diagram showing a multilayer culture vessel operating device 10a according to the present embodiment. As shown in FIG. 18, the multilayer culture vessel operation device 10a includes a rotation unit 11, a rotation drive unit 12, a drive control unit 15, an operation unit 16, an arm drive unit 17, a pair of arms 18, and a main body. 19 and. The drive control unit 15 introduces a culture solution introduction process for introducing a culture solution in which cells are seeded into the multi-layer culture container 30, a culture solution recovery process for recovering the culture solution from the multi-layer culture container 30, and a trypsin solution into the multi-layer culture container 30. An operation program for operating the multi-layer culture container 30, such as a tripsin introduction process, a cell exfoliation process for shaking the multi-layer culture container 30, and a tripsin solution recovery process for recovering the trypsin solution from the multi-layer culture container 30, is stored in advance.
The rotation drive unit 12 includes a first power motor that rotates the rotation unit 11 around the rotation shaft X1 and a second power motor that rotates the rotation unit 11 around the rotation shaft X2.

In the cell detachment treatment, the multi-layer culture container 30 is placed in the first direction (for example, to the right) and the second direction (for example, to the left) with the multi-layer culture container 30 centered on the first rotation axis or the second rotation axis by the rotating portion 11. Is programmed in the operation program to swing back and forth. The operation program is specified when switching from the rotation operation in the first direction to the rotation operation in the second direction and when switching from the rotation operation in the second direction to the rotation operation in the first direction. It has a stop mode for stopping the movement of the multilayer culture vessel 30 for a period of time.
By providing a stop mode, even if the swinging motion is performed at a speed faster than the movement of the liquid in the container, the swinging motion is stopped for a specified time when the direction of the rotation motion is switched, so that the liquid in the container can be stored in the container. It is possible to reliably collide with the side surface (side wall) of the. In order to effectively perform the cell detachment treatment, it is important to shake the container at high speed, but the problem of liquid movement delay (time lag) that occurs when the container is shaken at high speed is solved. It is possible.

The operation program executes an operation of collecting cells attached to the side surface of the tray 31 before the trypsin solution recovery process. When the tray 31 is a rectangular tray having the first to fourth side surfaces, the trypsin solution is rotated so as to collide with the first side surface, the second side surface, the third side surface, and the fourth side surface in this order. Operate unit 11. By performing such an operation, it becomes possible to collect the cells attached to the side surface of the tray 31 in the trypsin solution and then perform the trypsin solution recovery process.

The multi-layer culture container operating device 10a according to the second embodiment described above also has the same effect as that of the first embodiment.
Further, in the second embodiment, the cell detachment treatment can be performed without the shaking portion 13, and the manufacturing cost can be reduced because the apparatus configuration is simple.
Furthermore, by providing the stop mode, it is possible to solve the problem of the delay in the movement of the liquid (time lag) that occurs when the container is shaken at high speed.

Although the preferred embodiment of the present invention has been described above, the technical scope of the present invention is not limited to the description of the above embodiment. Various changes and improvements can be added to the above-described embodiments, and those in which such changes or improvements have been made are also included in the technical scope of the present invention.

In the above-described embodiment, the configuration in which the multilayer culture vessel operating system 1 or the multilayer culture vessel operating devices 10 and 10a are used for cell culture is exemplified, but the configuration is not limited to this configuration, and the multilayer culture vessel operating system 1 or the multilayer culture vessel operating system 1 or the multilayer culture vessel operation is not limited to this configuration. The devices 10 and 10a can also be configured to be used for culturing microorganisms.

Further, in addition to the above-described embodiment, the multi-layer culture container operating devices 10 and 10a may have the following configurations.
That is, when the rotation drive unit 12 and the arm drive unit 17 are configured by an air cylinder, the multilayer culture vessel operating devices 10 and 10a may be configured to include a pressure sensor that monitors the pressure of the air cylinder. can. Then, by monitoring the air pressure of the air cylinder with the pressure sensor, it is possible to detect the failure of the air cylinder.
Further, the multilayer culture vessel operating devices 10 and 10a may be configured to include an area sensor. Then, the safety can be improved by constantly monitoring the entry of a person into the vicinity of the multilayer culture vessel operating devices 10 and 10a, particularly the periphery of the rotating portion 11, by using the area sensor.
Further, the drive control unit 15 may be configured to count the number of operations of the rotating unit 11, the shaking unit 13, or the arm 18. Then, the drive control unit 15 replaces the electric power motor and the air cylinder constituting the rotation drive unit 12, the shaking drive unit 14, and the arm drive unit 17 based on the number of operations of the rotation unit 11, the shaking unit 13, or the arm 18. It can be configured to predict the time.
In addition, the drive control unit 15 may be configured to integrate the operating times of the rotating unit 11, the shaking unit 13, or the arm 18. Then, the drive control unit 15 may be configured to predict the replacement time of the AC / DC power supply, the battery, the fan, etc. based on the operating time of the rotating unit 11, the shaking unit 13, or the arm 18.

1 ... Multi-layer culture container operation system 10, 10a ... Multi-layer culture container operation device 11 ... Rotating part 111 ... Locking member
112 ... Recess
113 ... Tapered part
114 ... Groove 12 ... Rotational drive 17 ... Arm drive 13 ... Shaking 131 ... Top surface 14 ... Shaking drive 15 ... Drive control 16 ... Operation 18 ... Arm 181 ... Clamp 19 ... Main body 20 ... Bogie device 21 ... Bogie 22 ... Wheel 23 ... Fixing member 24 ... Pedestal 25 ... Frame member 26 ... Fastening member 27 ... Insertion hole 28 ... Locked part 30 ... Multilayer culture container 31 ... Tray 32 ... Vent cap

Claims (9)

It is a multi-layer culture container operation system having a trolley device that can be moved by mounting a multi-layer culture container having a plurality of trays, and an operation device that can hold and rotate the multi-layer culture container.
The operating device is
The multi-layer culture vessel can be rotated around the first axis and the second axis substantially orthogonal to the first axis.
By rotating the multi-layer culture container around the second axis at the same time as rotating the multi-layer culture container around the first axis, reciprocating rotation around the first axis and the second axis. A multi-layer culture vessel operation system that can swing back and forth. It is a multi-layer culture container operation system having a trolley device that can be moved by mounting a multi-layer culture container having a plurality of trays, and an operation device that can hold and rotate the multi-layer culture container.
The operating device can reciprocate and reciprocate to rotate the multilayer culture vessel along the first plane, the second plane, and the third plane that are substantially orthogonal to each other, and the operation device can reciprocate and swing along the first axis. By reciprocating the multi-layered culture vessel, the multi-layered culture vessel is reciprocally rotated along the first plane, and the multi-layered culture vessel is reciprocated on the second axis substantially orthogonal to the first axis. A multi-layer culture vessel operation system that reciprocates to rotate the multi-layer culture vessel reciprocating along the second plane by moving . The operating device is
A first operation unit that swings back and forth along the first plane and the second plane, and
The multi-layer culture vessel operation system according to claim 2, further comprising a second operation unit that swings back and forth along the third plane, which is different from the first operation unit. The multi-layer culture vessel operation system according to claim 3, wherein the first operation unit and the second operation unit are arranged in the vertical direction. 3. The second operation unit can be accommodated in the operation device main body, and when the reciprocating swing along the third plane is performed, the second operation unit is projected from the operation device main body. Or the multi-layer culture vessel operating system according to 4 . A multi-layer culture container operation system having a movable trolley equipped with a multi-layer culture container containing a plurality of trays and an operating device capable of holding and rotating the multi-layer culture container with an arm.
The dolly has an insertion hole through which the arm of the operating device is inserted.
The operating device is
A rotating unit that holds the multi-layer culture container and rotates the multi-layer culture container on the first rotation axis and / or the second rotation axis.
A shaking unit that shakes the multi-layer culture vessel in the horizontal direction, and a shaking portion.
It has a control unit that controls the operation of the rotating unit and the shaking unit, and has.
The arm is inserted into the insertion hole of the multi-layer culture container to hold the multi-layer culture container, and the control unit holds the multi-layer culture container with the arm and performs the rotation operation by the rotating unit, and the arm is used. A multi-layer culture vessel operation system that allows the shaking portion to perform the shaking operation while being inserted and held in the insertion hole. In the multi-layer culture vessel operation system according to claim 6 ,
The shaking part can be housed in the main body of the operating device.
The control unit is a multi-layer culture vessel operation system that projects the shaking unit from the main body of the operating device when the shaking operation is performed. A support member that vertically supports a multi-layer culture container containing multiple trays with side surfaces,
A locking member that cooperates with the support member to fix the multilayer culture vessel, and
A rotating portion that rotates the support member on the first rotation shaft and the second rotation shaft, and a rotating portion.
It has a support member and a control unit that controls the operation of the rotating unit.
The control unit reciprocates the multilayer culture vessel in the first direction and the second direction around the first rotation axis or the second rotation axis to execute the cell detachment treatment on the rotation unit. Cell detachment function and
When switching from the rotation motion in the first direction to the rotation motion in the second direction and when switching from the rotation motion in the second direction to the rotation motion in the first direction while performing the cell detachment function. , A multilayer culture vessel operating device having a stop mode for stopping the movement of the multilayer culture vessel for a specified time. A support member that movably supports a multi-layer culture container containing a plurality of trays having side surfaces, a locking member that cooperates with the support member to fix the multi-layer culture container, and a first rotation of the support member. The multi-layer culture into which a tripsin solution was introduced was introduced using an operating device having a rotating portion that rotates on a shaft and a second rotating shaft, and a control unit that controls the operation of the support member and the rotating portion. It is a method of operating a multi-layer incubator that operates a container.
During the cell detachment treatment, the multilayer culture vessel is reciprocally swung around the first rotation axis or the second rotation axis in the first direction and the second direction in the rotating portion. When switching from the rotation operation in the direction to the rotation operation in the second direction and when switching from the rotation operation in the second direction to the rotation operation in the first direction, the multi-layer culture container is used for a specified time. Multilayer culture container operation method to stop the movement.

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