JP7072830B2 - Manufacturing method and kit for manufacturing gel for electrophoresis with high separability - Google Patents

Description

The present invention relates to a method for producing an electrophoresis gel, particularly an electrophoresis gel having an improved resolution and a high resolution, a kit for producing the same, and the like.

When separating nucleic acids or proteins by electrophoresis, polyacrylamide gels or agarose gels are mainly used. When separating nucleic acids such as DNA, agarose gels are generally often used.

When separating DNAs of various sizes using an agarose gel, it is necessary to prepare an agarose gel adjusted to a suitable gel concentration according to the separating ability of the agarose gel, and the operation is very complicated. Further, in order to separate DNA having a large size exceeding 25 kbp, it is necessary to reduce the agarose gel concentration to less than 0.5% by weight, but this does not have sufficient strength of the gel and it is difficult to use it for electrophoresis. There was a case.

Therefore, the development of an electrophoresis gel having sufficient strength and high resolution is required, and various studies have been conducted. For example, Non-Patent Document 1 discloses a technique for producing a gel by combining agarose with a specific polysaccharide. Further, Patent Document 1 discloses a technique for combining galactomannan irradiated with gamma rays with agarose.

International Publication No. 92/12789 (released on August 6, 1992)

Perlman, D. et. Al., Anal. Biochem. 163, p.247-254, 1987

However, the prior art as described above has room for improvement from the viewpoint of easily obtaining a gel having sufficient strength and high separation ability.

Normally, when preparing a gel for electrophoresis such as agarose gel, a high-concentration solution such as agarose is prepared, and the high-concentration solution is diluted with a buffer or the like so as to finally reach a desired gel concentration. A gel for electrophoresis is produced by gelling this. In the left column of page 252 of Non-Patent Document 1, it is stated that in order to practically use a high concentration galactomannan exceeding 0.5%, it is necessary to partially hydrolyze the galactomannan. Has been done. The same page states that this is due to the rubbery hardness of the galactomannan-agarose solution and the high gel temperature. Therefore, in the technique described in Patent Document 1, it is necessary to irradiate galactomannan with gamma rays in advance in order to reduce the viscosity of the galactomannan solution. Therefore, it becomes a technical problem that an electrophoresis gel containing agarose and galactomannan cannot be easily prepared, and those skilled in the art practically use an electrophoresis gel containing agarose and galactomannan. It never happened.

One aspect of the present invention has been made in view of the above problems, and an object thereof is to realize a method for easily obtaining a gel having sufficient strength and high separation ability.

As a result of diligent research conducted by the present inventor in order to solve the above problems, surprisingly, a gel having sufficient strength and high separability by mixing agarose and galactomannan in a powder state in advance. We have found that can be obtained extremely easily, and have completed the present invention. The present invention includes the following aspects.

<1> A step of obtaining a mixed powder by mixing a powder containing agarose and a powder of galactomannan, a step of obtaining a solution by dissolving the mixed powder in a buffer solution, and a step of gelling the solution. A method for producing an electrophoresis gel, which comprises a step of obtaining an electrophoresis gel.

<2> The electricity according to <1>, wherein the weight ratio of the galactomannan powder to the agarose-containing powder in the above mixed powder (weight of the galactomannan powder / weight of the powder containing agarose) is 0.3 to 2. A method for producing a gel for migration.

<3> The method for producing an electrophoresis gel according to <1> or <2>, wherein the buffer solution is tris-acetic acid-ethylenediaminetetraacetic acid buffer solution or tris-boric acid-ethylenediaminetetraacetic acid buffer solution.

<4> A mixed powder containing a powder containing agarose and a powder of galactomannan.

<5> The mixed powder according to <4>, wherein the weight ratio of the galactomannan powder to the agarose-containing powder (weight of the galactomannan powder / weight of the powder containing agarose) is 0.3 to 2.

<6> An electrophoresis gel production kit comprising the mixed powder according to <4> or <5>.

<7> The gel production kit for electrophoresis according to <6>, further comprising a Tris-acetic acid-ethylenediaminetetraacetic acid buffer solution or a Tris-boric acid-ethylenediaminetetraacetic acid buffer solution.

According to one aspect of the present invention, there is an effect that an electrophoresis gel having sufficient strength and high separability can be easily produced.

It is a figure which shows the result of the electrophoresis of Reference Example 1 and Example 1. FIG. It is a figure which shows the result of the electrophoresis of the reference example 2 and the reference example 3. It is a figure which shows the result of the electrophoresis of Example 2, Example 3 and Example 4. FIG. It is a figure which shows the result of the electrophoresis of Example 5 and Comparative Example 1.

Hereinafter, an example of an embodiment of the present invention will be described in detail, but the present invention is not limited thereto. Unless otherwise specified in the present specification, "A to B" representing a numerical range means "A or more and B or less".

In the present specification, the gel for electrophoresis may be simply referred to as "gel". It can be said that the greater the distance between the bands of nucleic acids having different sizes in the gel, the higher the separation ability.

[1. Manufacturing method of gel for electrophoresis]
The method for producing an electrophoresis gel according to an embodiment of the present invention includes a step of obtaining a mixed powder by mixing a powder containing agarose and a powder of galactomannan, and a step of dissolving the mixed powder in a buffer solution. It includes a step of obtaining a solution and a step of obtaining a gel for electrophoresis by gelling the above solution. By mixing galactomannan with agarose, the separability and strength of the obtained gel can be improved. Further, by mixing agarose and galactomannan in the powder state, the mixed powder can be uniformly dissolved in the buffer solution. Therefore, a gel having sufficient separability and strength can be easily prepared. According to one embodiment of the present invention, it is not necessary to irradiate galactomannan with gamma rays.

On the other hand, when a solution in which agarose is dissolved and a solution in which galactomannan is dissolved are prepared separately and mixed, a gel having sufficient strength cannot be prepared. For example, when galactomannan is dissolved at a high concentration, galactomannan does not dissolve uniformly, which may make it difficult to prepare a gel. Further, as described above, the galactomannan solution may have a high viscosity. A gel containing only agarose at a low concentration without containing galactomannan may have insufficient strength.

Hereinafter, the method for producing an electrophoresis gel is also simply referred to as a “production method”. Further, in the present specification, a powder obtained by mixing a powder containing agarose and a powder of galactomannan is also simply referred to as "mixed powder".

<1-1. Mixing process>
The above-mentioned production method includes a step of obtaining a mixed powder by mixing a powder containing agarose and a powder of galactomannan. That is, the mixed powder according to the embodiment of the present invention contains a powder containing agarose and a powder of galactomannan. As used herein, this step is also referred to as a "mixing step".

The powder containing agarose preferably contains 70% by weight or more of agarose, more preferably 80% by weight or more, and further preferably 90% by weight or more in 100% by weight. The powder containing agarose may be a powder made of agarose (agarose powder) or agar powder. The agar powder is an agar powder and may contain polysaccharides other than agarose to the extent that it does not inhibit DNA analysis, in addition to agarose.

Agarose is a polysaccharide having a structure in which β-D-galactose bound at the 1-position and the 3-position and 3,6-anhydro-α-L-galactose bound at the 1-position and the 4-position are alternately bound. Is. In other words, agarose has a structure in which a repeating unit in which D-galactose is bound to 3,6-anhydro-L-galactose by β1 → 4 is bound by α1 → 3. Examples of commercially available agarose powder include Agarose H (manufactured by Nippon Gene Co., Ltd.) and Agarose S (manufactured by Nippon Gene Co., Ltd.).

As the powder containing agarose, agarose powder and agar powder may be used in combination. The weight ratio of the agarose powder to the agar powder is not particularly limited, but the agarose powder: agar powder = 2.3: 1 to 4: 1 may be used.

Galactomannan is a polysaccharide having a structure in which D-galactose is bound to α1 → 6 on a linear main chain formed by binding D-mannose to β1 → 4. Galactomannan can be extracted from locust beans, guar, fenugreek and the like. Examples of commercially available galactomannan powder include AgarMate (registered trademark) (manufactured by Diversified Biotech) and Locust bean gum from Ceratonia siliqua seeds (manufactured by Sigma-Aldrich), and AgarMate is preferable.

The weight ratio of the galactomannan powder to the agarose-containing powder in the mixed powder (weight of the galactomannan powder / weight of the powder containing agarose) is preferably 0.3 to 2. When the weight ratio is 0.3 or more, the separability and strength of the obtained gel can be further enhanced. Further, when the weight ratio is 2 or less, the mixed powder is easily dissolved in the buffer solution.

The weight ratio may be, for example, 0.8 to 2 or 1 to 2. Even when the concentration of galactomannan is relatively high as described above, the mixed powder is sufficiently dissolved in the buffer solution. Therefore, a gel having sufficient strength can be obtained. This is because in one embodiment of the present invention, agarose and galactomannan are mixed in a powder state. Further, the weight ratio may be 0.3 to 0.6 or 0.3 to 0.5. Even when the concentration of galactomannan is relatively low as described above, the separation ability of the gel can be sufficiently enhanced.

The method of mixing the powder containing agarose and the powder of galactomannan is not particularly limited. These powders may be mixed manually or may be mixed by an apparatus provided with a stirring mechanism or the like. In any case, it is preferable to stir the powder sufficiently so that the powder containing agarose and the powder of galactomannan are uniformly mixed.

<1-2. Dissolution process>
The production method comprises a step of obtaining a solution by dissolving the mixed powder in a buffer solution. As used herein, this step is also referred to as a "dissolution step". In the above-mentioned mixing step, the powder containing agarose and the powder of galactomannan are already mixed. Therefore, in the dissolution step, the mixed powder can be uniformly dissolved in the buffer solution. The uniform solution thus obtained is easy to handle in the gelation step described later.

The buffer is preferably Tris-acetic acid-ethylenediaminetetraacetic acid buffer or Tris-boric acid-ethylenediaminetetraacetic acid buffer.

Tris-acetic acid-ethylenediaminetetraacetic acid buffer is also referred to as TAE buffer. TAE buffer can be prepared by dissolving trishydroxymethylaminomethane, acetic acid and ethylenediaminetetraacetic acid (EDTA) in water. Tris hydroxymethylaminomethane is also simply referred to as Tris. Tris may be used in the form of salts (eg, Tris-HCL). Acetic acid may also be used in the form of acetate (eg, sodium acetate). TAE buffer is preferred from the standpoint of providing for DNA recovery and genetic engineering techniques.

Tris-boric acid-ethylenediaminetetraacetic acid buffer is also referred to as TBE buffer. TBE buffer can be prepared by dissolving trishydroxymethylaminomethane, boric acid and EDTA in water. From the viewpoint of separating DNA of 1 kbp or less, TBE buffer is preferable.

The total amount of the agarose-containing powder and the galactomannan powder added in 100% by weight of the solution is preferably 0.4 to 1.5% by weight, and preferably 0.4 to 1.0% by weight. More preferred. When the above concentration is 0.4% by weight or more, the separation ability and strength of the gel can be sufficiently improved. When the above concentration is 1.5% by weight or less, a uniform solution can be easily obtained.

The amount of the powder containing agarose added in 100% by weight of the solution is preferably 0.3 to 1.5% by weight. When the amount of the powder containing agarose added is 0.3% by weight or more, a gel having sufficient strength can be obtained. When the amount of the powder containing agarose added is 1.5% by weight or less, a uniform solution can be easily obtained.

The amount of the galactomannan powder added in 100% by weight of the solution is preferably 0.1 to 0.6% by weight. When the amount of the galactomannan powder added is 0.1% by weight or more, the separation ability and strength of the gel can be sufficiently improved. When the amount of the galactomannan powder added is 0.6% by weight or less, a uniform solution can be easily obtained.

It is preferable to put the mixed powder into the buffer solution and then stir. It may be stirred manually or by a device equipped with a stirring mechanism. Examples of the device provided with the stirring mechanism include a magnetic stirrer and the like.

In the dissolution step, it is preferable to dissolve the mixed powder in the buffer solution while heating. This makes it possible to easily dissolve the mixed powder in the buffer solution. The heating temperature in the melting step is preferably 80 to 100 ° C. When the heating temperature is in the above range, the mixed powder can be sufficiently dissolved. The heating can be performed using a microwave oven or the like, which is usually used for producing an agarose gel.

Whether or not the mixed powder is uniformly dissolved can be visually confirmed. In the present specification, if there is no lump of agarose or agar or galactomannan remaining undissolved in the above solution, it is determined that the solution is uniformly dissolved.

<1-3. Gelling process>
The production method includes a step of obtaining a gel for electrophoresis by gelling the solution. As used herein, this step is also referred to as a "gelling step". As used herein, "gelling" is intended to obtain a gel that does not exhibit fluidity by solidifying the solution. The above gel is a gelled solution of the above-mentioned mixed powder. Therefore, the gel has sufficient strength and high resolution.

In the gelling step, it is preferable to solidify the solution by leaving it to stand. The temperature at which it is left to stand is preferably 20 to 60 ° C., more preferably room temperature. Here, a gel having a desired shape can be obtained by pouring the solution into a mold and then leaving it to stand. It is also possible to form a well for introducing a sample by inserting a comb into the above solution before solidification.

[2. Gel production kit for electrophoresis]
The gel production kit for electrophoresis according to an embodiment of the present invention comprises a mixed powder according to an embodiment of the present invention. Therefore, as described above, a gel having sufficient strength and high separability can be easily prepared. Hereinafter, the gel production kit for electrophoresis is also simply referred to as a “kit”. In addition, [1. Regarding the matters already described in [Method for producing a gel for electrophoresis], the description thereof will be omitted below, and the above description will be appropriately incorporated.

The kit may further include a TAE buffer or a TBE buffer as the buffer. A solution can be obtained by dissolving the above-mentioned mixed powder in the buffer solution. A gel can be prepared by gelling the solution.

The buffer solution may be a stock solution prepared at a concentration higher than the concentration at the time of use. For example, the stock solution may be 5 times, 10 times, or 50 times the concentration at the time of use. The stock solution can be diluted and used to make a gel.

The kit may also include tris-acetic acid-ethylenediaminetetraacetic acid powder or tris-boric acid-ethylenediaminetetraacetic acid powder. By dissolving these powders in water, a TAE buffer solution or a TBE buffer solution can be obtained.

In addition to the mixed powder and buffer, the kit may include members for making a gel. For example, the kit may include a tray into which the solution is poured. The kit may also include a plate to support the formed gel. In addition, the kit may include a comb to form a well in the gel.

The present invention is not limited to the above-described embodiments, and various modifications can be made within the scope of the claims, and the embodiments obtained by appropriately combining the technical means disclosed in the different embodiments. Is also included in the technical scope of the present invention.

One aspect of the present invention may be configured as follows.

<1> A step of mixing an agarose powder and a galactomannan powder to obtain an agarose-galactomannan mixed powder, and a step of dissolving the above-mentioned agarose-galactomannan mixed powder in a buffer solution to obtain an agarose-galactomannan solution. , A method for producing an electrophoresis gel, which comprises a step of gelling the above agarose-galactomannan solution to obtain an electrophoresis gel.

<2> The weight ratio of the galactomannan powder to the agarose powder in the above agarose-galactomannan mixed powder (weight of the galactomannan powder / weight of the agarose powder) is 0.3 to 2, according to <1>. Method for producing a gel for electrophoresis.

<3> The method for producing an electrophoresis gel according to <1> or <2>, wherein the buffer solution is tris-acetic acid-ethylenediaminetetraacetic acid buffer solution or tris-boric acid-ethylenediaminetetraacetic acid buffer solution.

<4> Agarose-galactomannan mixed powder containing agarose powder and galactomannan powder.

<5> The agarose-galactomannan mixture according to <4>, wherein the weight ratio of the galactomannan powder to the agarose powder (weight of the galactomannan powder / weight of the agarose powder) is 0.3 to 2. Powder.

<6> A gel production kit for electrophoresis comprising the agarose-galactomannan mixed powder according to <4> or <5>.

<7> The gel production kit for electrophoresis according to <6>, further comprising a Tris-acetic acid-ethylenediaminetetraacetic acid buffer solution or a Tris-boric acid-ethylenediaminetetraacetic acid buffer solution.

Hereinafter, the present invention will be described in more detail based on examples, but the present invention is not limited to the following examples.

[1. Electrophoresis method using agarose powder]
[1-1. Electrophoresis method]
The electrophoresis method commonly performed in Examples 1 to 4 and Reference Examples 1 to 3 described later will be described.

(1) Electrophoresis The gel was set in an electrophoresis device (Mupid mini gel electrophoresis tank, manufactured by Mupid (formerly Advance)). 3-5 μL of DNA size marker was added to the wells formed in the gel. The following (a) to (d) were used as DNA size markers.
(A) λHIII marker (manufactured by Nippon Gene) plus 550 bp and 720 bp DNA prepared by the inventor (b) 1 to 10 kb of DNA (1-10 kb DNA Markers, manufactured by FMC).
(C) 0.1 to 10 kb of DNA (Gene Ladder Fast2, manufactured by Nippon Gene)
(D) 0.1 to 2 kb of DNA (Gene Ladder 100, manufactured by Nippon Gene)
Electrophoresis was performed at room temperature for 30 minutes at 100 V. As the migration buffer, TAE buffer (pH 8.0; 40 mM Tris-HCl, 20 mM sodium acetate, 2 mM EDTA) was used. The electrophoresis was carried out by a conventional method with reference to Molecular Cloning: A LABORATORY MANUAL, T. Maniatis, EF Fritsch, J. Sambrook, Cold Spring Harbor Laboratory, 1982.

(2) Staining of gel The gel after electrophoresis was immersed in a TAE buffer solution in which ethidium bromide (0.5 μg / mL) was dissolved for 15 minutes. Further, the gel was immersed in distilled water for 5 minutes.

(3) Photographing the gel The gel was photographed using an agarose gel photographing apparatus (UV illuminator; Model BioDoc-It (registered trademark) Imaging system, manufactured by UVP).

[1-2. Comparison of the presence or absence of galactomannan mixture]
<Reference example 1>
(1) Dissolution of agarose In a 200 mL glass bottle with a lid, 100 mL of TAE buffer (pH 8.0; 40 mM Tris-HCl, 20 mM sodium acetate, 2 mM EDTA) and stirrer bar were placed. 0.5 g of agarose (Agarose H, manufactured by Nippon Gene Co., Ltd.) was put into the glass bottle, covered with a lid, and shaken quickly up and down about 2 to 3 times. Using a microwave oven, the glass bottle was heated at 100 ° C. for about 2 minutes to dissolve the agarose. Then, the glass bottle was placed on a stirrer and stirred. This gave an agarose solution. It was confirmed that the agarose was completely dissolved in the agarose solution.

(2) Preparation of gel The sample comb (for 8 wells) and the gel plate (width 54 mm, length 60 mm, height 10 mm) attached to the above-mentioned electrophoresis device were set in the gel preparation trays, respectively. 13 mL of agarose solution was poured into the gel preparation tray. The agarose solution was left at room temperature for 1 hour to solidify.

A small amount of TAE buffer was placed on the solidified gel and the sample comb was carefully removed. The gel was prepared in this way.

<Example 1>
0.3 g of agarose (Agarose H, manufactured by Nippon Gene) and 0.1 g of galactomannan (AgarMate® (CAT No. AGM 200), manufactured by Diversified Biotech) were weighed respectively. These were well stirred and mixed using a spatula.

A gel was prepared in the same manner as in Reference Example 1 except that 0.4 g of the obtained mixed powder was used instead of 0.5 g of the above-mentioned agarose.

<Electrophoresis>
The gels of Reference Example 1 and Example 1 were electrophoresed at the same time. FIG. 1 is a diagram showing the results of electrophoresis of Reference Example 1 and Example 1. The scale representing the size of the DNA shown in FIG. 1 is based on the band of Reference Example 1. The concentration range of commonly used agarose is 0.5 to 2% by weight. Example 1 contains 0.3% by weight agarose, which is a lower concentration. Nevertheless, in Example 1, the same separability as in Reference Example 1 was obtained. In Reference Example 1 and Example 1, it can be seen that the differences in 1 kbp are well separated from 1 to 23 kbp.

When 0.3% by weight of agarose was used alone, an extremely fragile gel was obtained and it could not be used. On the other hand, in Example 1, the strength of the gel was increased by the addition of galactomannan.

[1-3. Comparison of agarose content]
<Reference example 2>
A gel was prepared in the same manner as in Reference Example 1.

<Reference example 3>
A gel was prepared in the same manner as in Reference Example 1 except that 1.5 g of agarose was used instead of 0.5 g of agarose.

<Electrophoresis>
The gels of Reference Example 2 and Reference Example 3 were electrophoresed at the same time. FIG. 2 is a diagram showing the results of electrophoresis of Reference Example 2 and Reference Example 3. In Reference Example 3 in which agarose was used at a higher concentration, the difference in marker d every 100 bp became clearer than in Reference Example 2. It can be seen that in Reference Example 3, the resolution was improved as compared with Reference Example 2.

[1-4. Comparison of galactomannan content]
<Example 2>
A gel was prepared in the same manner as in Example 1.

<Example 3>
A gel was prepared in the same manner as in Example 1 except that 0.3 g of galactomannan was used instead of 0.1 g of galactomannan.

<Example 4>
A gel was prepared in the same manner as in Example 1 except that 0.6 g of galactomannan was used instead of 0.1 g of galactomannan.

<Electrophoresis>
The gels of Example 2, Example 3 and Example 4 were electrophoresed at the same time. FIG. 3 is a diagram showing the results of electrophoresis of Example 2, Example 3, and Example 4. Comparing Example 2, Example 3 and Example 4, as the concentration of galactomannan was increased to 0.1% by weight, 0.3% by weight and 0.6% by weight, the difference in marker d for each 100bp was increased. It turned out to be clearer. Reference Example 3 above is a gel containing 1.5% by weight of agarose, which is usually used in the laboratory. Compared with the gel of Reference Example 3, in Examples 2 to 4, much higher separation ability is shown at 100 to 2000 bp. The region of 100 to 2000 bp is a region often used for analysis of the result of PCR reaction. Therefore, it is strongly suggested that the method for producing an electrophoresis gel according to an embodiment of the present invention may be general-purpose in the future. The gels of Examples 2 to 4 were all excellent in strength.

[1-5. summary]
The concentrations of agarose and galactomannan in the gels used in Reference Examples 1 to 3 and Examples 1 to 4 are shown in Table 1 below.

Conventionally, agarose gels having various concentrations have been used depending on the length of DNA to be separated. Then, the most suitable agarose product for each concentration is commercially available. According to the method for producing an electrophoresis gel according to an embodiment of the present invention, DNA from 100 bp to 23 kbp can be easily separated by using a powder obtained by mixing agarose powder and galactomannan powder. Therefore, it is not necessary to prepare different agarose products depending on the concentration of the agarose gel to be produced.

[2. Electrophoresis method using agar powder]
[2-1. Electrophoresis method]
In Example 5 and Comparative Example 1 described later, except that the following (e) and (f) were used as DNA size markers, [1-1. Electrophoresis was performed in the same manner as in [Electrophoresis method].
(E) 0.1 to 20 kb of DNA (Gene ladder wide 2, manufactured by Nippon Gene)
(F) 0.1 to 2 kb of DNA (Gene Ladder 100, manufactured by Nippon Gene)
[2-2. Comparison of the presence or absence of galactomannan mixture]
<Example 5>
A gel was prepared in the same manner as in Example 1 except that 0.5 g of agar powder (purified agar powder, manufactured by Nakaraitesk Co., Ltd.) was used instead of 0.3 g of agarose and 0.5 g of galactomannan was used. Powder 0.5% by weight, galactomannan 0.5% by weight).

<Comparative Example 1>
A gel was prepared in the same manner as in Reference Example 1 except that agar powder (purified agar powder, manufactured by Nacalai Tesque) was used instead of agarose (0.5% by weight of agar powder).

<Electrophoresis>
The gels of Example 5 and Comparative Example 1 were electrophoresed at the same time. FIG. 4 is a diagram showing the results of electrophoresis of Example 5 and Comparative Example 1. Note that * in the figure indicates the position of the 2 kb DNA band. It can be seen that 16 of the 18 DNA bands of the marker e of Example 5 and all 12 DNA bands of the marker f are completely separated. In Comparative Example 1, it can be seen that the DNA bands of 1 kb or more were separated from each of the markers e and f, but the DNA bands of 0.1 kb or more and less than 1 kb could not be separated.

These results indicate that the use of galactomannan is also extremely effective in separating small molecule regions in electrophoresis using agar powder instead of agarose. In addition, gels using agar powder are known to be fragile. However, the addition of galactomannan improved the strength of the gel. That is, the addition of galactomannan could also solve the problem of gel strength. Agar powder is cheaper than agarose. Therefore, it is expected that the use of agar powder will increase when combined with galactomannan.

The present invention can be used in various fields in which analysis involving electrophoresis is performed.

Claims (6)

A step of obtaining a mixed powder by mixing a powder containing agarose and a powder of galactomannan not irradiated with gamma rays .
The step of obtaining a solution by dissolving the above mixed powder in a buffer solution, and
A method for producing a nucleic acid electrophoresis gel, which comprises a step of obtaining a nucleic acid electrophoresis gel by gelling the above solution. The nucleic acid electrophoresis according to claim 1, wherein the weight ratio of the galactomannan powder to the agarose-containing powder in the above mixed powder (weight of the galactomannan powder / weight of the powder containing agarose) is 0.3 to 2. How to make gel. The method for producing a gel for nucleic acid electrophoresis according to claim 1 or 2, wherein the buffer solution is Tris-acetic acid-ethylenediaminetetraacetic acid buffer solution or Tris-boric acid-ethylenediaminetetraacetic acid buffer solution. A gel production kit for nucleic acid electrophoresis comprising a mixed powder containing a powder containing agarose and a powder of galactomannan not irradiated with gamma rays , and a buffer solution. The gel production for nucleic acid electrophoresis according to claim 4, wherein the weight ratio of the galactomannan powder to the agarose-containing powder (weight of the galactomannan powder / weight of the powder containing agarose) is 0.3 to 2. kit. The gel production kit for nucleic acid electrophoresis according to claim 4 or 5, wherein the buffer is Tris-acetic acid-ethylenediaminetetraacetic acid buffer or Tris-boric acid-ethylenediaminetetraacetic acid buffer.

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