JP7068384B2 - 生物学的アクティビティをアッセイするための微少流体デバイス - Google Patents

生物学的アクティビティをアッセイするための微少流体デバイス Download PDF

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JP7068384B2
JP7068384B2 JP2020089306A JP2020089306A JP7068384B2 JP 7068384 B2 JP7068384 B2 JP 7068384B2 JP 2020089306 A JP2020089306 A JP 2020089306A JP 2020089306 A JP2020089306 A JP 2020089306A JP 7068384 B2 JP7068384 B2 JP 7068384B2
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enclosure
micro
objects
captured
microfluidic device
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JP2020122804A5 (enExample
JP2020122804A (ja
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ティー. チャプマン,ケヴィン
マレオ,ダニエレ
ネヴィル,ジェイ.タナー
ダブリュ. ショート,スティーヴン
ピー. ホワイト,マーク
ロウレイロ,エム.ジメナ
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バークレー ライツ,インコーポレイテッド
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US201361996969P 2013-10-22 2013-10-22
US201361996973P 2013-10-22 2013-10-22
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US61/996,969 2013-10-22
US61/996,973 2013-10-22
US61/996,962 2013-10-22
US201462058658P 2014-10-01 2014-10-01
US62/058,658 2014-10-01
US14/520,510 US9617145B2 (en) 2013-10-22 2014-10-22 Exporting a selected group of micro-objects from a micro-fluidic device
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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060154361A1 (en) 2002-08-27 2006-07-13 Wikswo John P Bioreactors with substance injection capacity
JP2008136475A (ja) 2006-11-10 2008-06-19 Univ Waseda 細胞捕捉装置及びそれを利用した細胞操作方法
US20080274493A1 (en) 2000-11-16 2008-11-06 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US20090170186A1 (en) 2004-04-12 2009-07-02 Ming Chiang Wu Optoelectronic tweezers for microparticle and cell manipulation
WO2009130694A2 (en) 2008-04-21 2009-10-29 Cell Kinetics Ltd. Flat cell carriers with cell traps
JP2010060567A (ja) 2004-12-03 2010-03-18 Orion Diagnostica Oy 粒子に基づく結合アッセイ法
US20110262906A1 (en) 2008-10-10 2011-10-27 Dublin City University Microfluidic multiplexed cellular and molecular analysis device and method
WO2012037030A2 (en) 2010-09-14 2012-03-22 The Regents Of The University Of California Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1352093A4 (en) * 2000-11-28 2008-01-16 Univ California OPTICAL TRANSFER AND SORTING IN A MICROFLUIDIC DEVICE, INCLUDING DEVICES WITH INTEGRATED BIOCHIPS, TRANSPORTED BIOLOGICAL SAMPLES AND MICROPARTICLES
KR100597870B1 (ko) * 2004-04-01 2006-07-06 한국과학기술원 고속 스크리닝 또는 고속 분석을 위한 미소 유체 칩
CN102449163A (zh) * 2009-04-03 2012-05-09 加利福尼亚大学董事会 分选细胞和其它生物微粒的方法和装置

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080274493A1 (en) 2000-11-16 2008-11-06 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US20060154361A1 (en) 2002-08-27 2006-07-13 Wikswo John P Bioreactors with substance injection capacity
US20090170186A1 (en) 2004-04-12 2009-07-02 Ming Chiang Wu Optoelectronic tweezers for microparticle and cell manipulation
JP2010060567A (ja) 2004-12-03 2010-03-18 Orion Diagnostica Oy 粒子に基づく結合アッセイ法
JP2008136475A (ja) 2006-11-10 2008-06-19 Univ Waseda 細胞捕捉装置及びそれを利用した細胞操作方法
WO2009130694A2 (en) 2008-04-21 2009-10-29 Cell Kinetics Ltd. Flat cell carriers with cell traps
US20110262906A1 (en) 2008-10-10 2011-10-27 Dublin City University Microfluidic multiplexed cellular and molecular analysis device and method
WO2012037030A2 (en) 2010-09-14 2012-03-22 The Regents Of The University Of California Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Jian Chen et al,Microfluidic approaches for cancer cell detection, characterization, and separation,Lab on a Chip,2012年,12,1753-1767

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