JP7068384B2 - 生物学的アクティビティをアッセイするための微少流体デバイス - Google Patents
生物学的アクティビティをアッセイするための微少流体デバイス Download PDFInfo
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- JP7068384B2 JP7068384B2 JP2020089306A JP2020089306A JP7068384B2 JP 7068384 B2 JP7068384 B2 JP 7068384B2 JP 2020089306 A JP2020089306 A JP 2020089306A JP 2020089306 A JP2020089306 A JP 2020089306A JP 7068384 B2 JP7068384 B2 JP 7068384B2
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- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
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- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
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| US201462058658P | 2014-10-01 | 2014-10-01 | |
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| US14/520,510 US9617145B2 (en) | 2013-10-22 | 2014-10-22 | Exporting a selected group of micro-objects from a micro-fluidic device |
| US14/520,510 | 2014-10-22 |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060154361A1 (en) | 2002-08-27 | 2006-07-13 | Wikswo John P | Bioreactors with substance injection capacity |
| JP2008136475A (ja) | 2006-11-10 | 2008-06-19 | Univ Waseda | 細胞捕捉装置及びそれを利用した細胞操作方法 |
| US20080274493A1 (en) | 2000-11-16 | 2008-11-06 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
| US20090170186A1 (en) | 2004-04-12 | 2009-07-02 | Ming Chiang Wu | Optoelectronic tweezers for microparticle and cell manipulation |
| WO2009130694A2 (en) | 2008-04-21 | 2009-10-29 | Cell Kinetics Ltd. | Flat cell carriers with cell traps |
| JP2010060567A (ja) | 2004-12-03 | 2010-03-18 | Orion Diagnostica Oy | 粒子に基づく結合アッセイ法 |
| US20110262906A1 (en) | 2008-10-10 | 2011-10-27 | Dublin City University | Microfluidic multiplexed cellular and molecular analysis device and method |
| WO2012037030A2 (en) | 2010-09-14 | 2012-03-22 | The Regents Of The University Of California | Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1352093A4 (en) * | 2000-11-28 | 2008-01-16 | Univ California | OPTICAL TRANSFER AND SORTING IN A MICROFLUIDIC DEVICE, INCLUDING DEVICES WITH INTEGRATED BIOCHIPS, TRANSPORTED BIOLOGICAL SAMPLES AND MICROPARTICLES |
| KR100597870B1 (ko) * | 2004-04-01 | 2006-07-06 | 한국과학기술원 | 고속 스크리닝 또는 고속 분석을 위한 미소 유체 칩 |
| CN102449163A (zh) * | 2009-04-03 | 2012-05-09 | 加利福尼亚大学董事会 | 分选细胞和其它生物微粒的方法和装置 |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080274493A1 (en) | 2000-11-16 | 2008-11-06 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
| US20060154361A1 (en) | 2002-08-27 | 2006-07-13 | Wikswo John P | Bioreactors with substance injection capacity |
| US20090170186A1 (en) | 2004-04-12 | 2009-07-02 | Ming Chiang Wu | Optoelectronic tweezers for microparticle and cell manipulation |
| JP2010060567A (ja) | 2004-12-03 | 2010-03-18 | Orion Diagnostica Oy | 粒子に基づく結合アッセイ法 |
| JP2008136475A (ja) | 2006-11-10 | 2008-06-19 | Univ Waseda | 細胞捕捉装置及びそれを利用した細胞操作方法 |
| WO2009130694A2 (en) | 2008-04-21 | 2009-10-29 | Cell Kinetics Ltd. | Flat cell carriers with cell traps |
| US20110262906A1 (en) | 2008-10-10 | 2011-10-27 | Dublin City University | Microfluidic multiplexed cellular and molecular analysis device and method |
| WO2012037030A2 (en) | 2010-09-14 | 2012-03-22 | The Regents Of The University Of California | Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices |
Non-Patent Citations (1)
| Title |
|---|
| Jian Chen et al,Microfluidic approaches for cancer cell detection, characterization, and separation,Lab on a Chip,2012年,12,1753-1767 |
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| JP2020122804A (ja) | 2020-08-13 |
| DK3060912T3 (da) | 2020-09-28 |
| JP2022093581A (ja) | 2022-06-23 |
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