JP7036197B2 - フィールドフローフラクショネーション装置 - Google Patents
フィールドフローフラクショネーション装置 Download PDFInfo
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- 238000001825 field-flow fractionation Methods 0.000 title claims description 25
- 239000012530 fluid Substances 0.000 claims description 183
- 238000000926 separation method Methods 0.000 claims description 102
- 239000007788 liquid Substances 0.000 claims description 52
- 239000012528 membrane Substances 0.000 claims description 36
- 239000002699 waste material Substances 0.000 claims description 28
- 239000002245 particle Substances 0.000 claims description 19
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims 1
- 239000010419 fine particle Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000004627 regenerated cellulose Substances 0.000 description 3
- 239000004695 Polyether sulfone Substances 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 229920006393 polyether sulfone Polymers 0.000 description 2
- QNRATNLHPGXHMA-XZHTYLCXSA-N (r)-(6-ethoxyquinolin-4-yl)-[(2s,4s,5r)-5-ethyl-1-azabicyclo[2.2.2]octan-2-yl]methanol;hydrochloride Chemical compound Cl.C([C@H]([C@H](C1)CC)C2)CN1[C@@H]2[C@H](O)C1=CC=NC2=CC=C(OCC)C=C21 QNRATNLHPGXHMA-XZHTYLCXSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000001249 flow field-flow fractionation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03B—SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS
- B03B5/00—Washing granular, powdered or lumpy materials; Wet separating
- B03B5/62—Washing granular, powdered or lumpy materials; Wet separating by hydraulic classifiers, e.g. of launder, tank, spiral or helical chute concentrator type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/0005—Field flow fractionation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/08—Flat membrane modules
- B01D63/088—Microfluidic devices comprising semi-permeable flat membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/123—Flexible; Elastomeric
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N2001/1006—Dispersed solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N2015/0038—Investigating nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/0005—Field flow fractionation
- G01N2030/0015—Field flow fractionation characterised by driving force
- G01N2030/0025—Field flow fractionation characterised by driving force cross flow FFF
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Hydrology & Water Resources (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Sampling And Sample Adjustment (AREA)
- Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)
Description
M1=M2+M3
となる。分級測定の際は、検出器20を流れる流体の流量M2(=M3-M1)が一定となるように送液ポンプ14の動作が制御される。クロスフロー流量M3はマスフローコントローラ26によって制御される。すなわち、送液ポンプ14は、マスフローコントローラ26による制御値と流量計15の測定値に基づいて、M3-M1が一定となるように制御される。
4 入口ポート
6 出口ポート
8 中間ポート
10 分離膜
12 サンプルインジェクション
14,16 送液ポンプ
15,17 流量計
18 キャリア流体用の容器
20 検出器
22 廃液チャンバ
24 排出ポート
26 マスフローコントローラ
28 排出流路
30 キャリア流体追加部
32 流路切替バルブ
34 フォーカスフロー形成用流路
36 キャリア流体追加用流路
Claims (7)
- 両端に入口ポートと出口ポートが設けられ、キャリア流体が流れる空間をなす分離チャネルと、
前記入口ポートを介して前記分離チャネルにキャリア流体を供給するキャリア流体供給部と、
キャリア流体を通過させつつ分離対象の粒子を通過させない性質をもち、前記分離チャネルを画する壁面をなす分離膜と、
前記分離チャネルを流れるキャリア流体のうち前記分離膜を通過したキャリア流体が流れる空間をなし、前記分離膜を通過したキャリア流体を外部へ排出する排出ポートを有する廃液チャンバと、
前記排出ポートに接続され、前記排出ポートを介して前記廃液チャンバから排出されるキャリア流体の流量を予め設定された流量に調節することにより、前記分離チャネル内のキャリア流体が前記分離膜を通過することによって形成されるクロスフローの流量を調節するクロスフロー流量調節部と、
前記クロスフロー流量調節部に流入するキャリア流体の流量が前記分離膜を通過したキャリア流体の流量よりも大きくなるように、前記クロスフロー流量調節部よりも上流側に設定されたキャリア流体追加位置において、前記分離膜を通過したキャリア流体の流れに別のキャリア流体の流れを追加するキャリア流体追加部と、を備えたフィールドフローフラクショネーション装置。 - 前記キャリア流体追加位置は、前記分離膜を通過したキャリア流体の流れ方向における前記分離膜と前記クロスフロー流量調節部との間の位置に設定されている、請求項1に記載のフィールドフローフラクショネーション装置。
- 前記キャリア流体追加位置は、前記排出ポートと前記クロスフロー流量調節部との間に設定されている請求項2に記載のフィールドフローフラクショネーション装置。
- 前記キャリア流体追加位置は、前記廃液チャンバの前記排出ポートが設けられている位置とは反対側の位置に設定されている請求項2に記載のフィールドフローフラクショネーション装置。
- 前記分離チャネルの前記入口ポートとは別のキャリア流体供給位置に接続され、所定のタイミングで前記分離チャネルにキャリア流体を供給して、前記入口ポートからのキャリア流体の流れと対向するキャリア流体の流れを前記分離チャネル内に形成するフォーカスフロー形成部を備え、
前記フォーカスフロー形成部は前記キャリア流体追加部としての機能を有し、前記キャリア流体供給位置から前記分離チャネルにキャリア流体を供給するタイミングとは別のタイミングで、前記キャリア流体追加位置において前記分離膜を通過したキャリア流体の流れにキャリア流体の流れを供給するように構成されている請求項1に記載のフィールドフローフラクショネーション装置。 - 前記フォーカスフロー形成部はキャリア流体を送液する送液ポンプを備え、前記送液ポンプが前記キャリア流体供給位置と前記キャリア流体追加位置に流路切替バルブを介して接続され、前記流路切替バルブの切替えによって、キャリア流体の供給先が前記キャリア流体供給位置と前記キャリア流体追加位置のいずれか一方に選択的に切り替えられるように構成されている請求項5に記載のフィールドフローフラクショネーション装置。
- 前記クロスフロー流量調節部はマスフローコントローラである請求項1から6のいずれか一項に記載のフィールドフローフラクショネーション装置。
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PCT/JP2018/015241 WO2019198178A1 (ja) | 2018-04-11 | 2018-04-11 | フィールドフローフラクショネーション装置 |
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US (1) | US11819859B2 (ja) |
EP (1) | EP3778031A4 (ja) |
JP (1) | JP7036197B2 (ja) |
CN (1) | CN111971127B (ja) |
WO (1) | WO2019198178A1 (ja) |
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US20220146464A1 (en) * | 2020-10-14 | 2022-05-12 | Wyatt Technology Corporation | Performing a separation on a field flow fractonator |
Citations (4)
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JP2008000724A (ja) | 2006-06-26 | 2008-01-10 | Shimadzu Corp | フィールドフローフラクショネーション装置 |
JP2008200566A (ja) | 2007-02-16 | 2008-09-04 | Shimadzu Corp | フィールドフローフラクショネーション装置 |
US20160238571A1 (en) | 2015-02-13 | 2016-08-18 | Postnova Analytics Gmbh | Apparatus for Field-Flow Fractionation |
WO2018056166A1 (ja) | 2016-09-20 | 2018-03-29 | 株式会社島津製作所 | フィールドフローフラクショネーション装置 |
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US6306531B1 (en) * | 1999-07-06 | 2001-10-23 | General Motors Corporation | Combustor air flow control method for fuel cell apparatus |
US20040000519A1 (en) * | 2002-06-28 | 2004-01-01 | Postnova Analytics, Inc. | Field-flow fractionation method and apparatus |
CN101344274B (zh) | 2008-08-25 | 2011-06-15 | 北京硕人时代科技有限公司 | 锅炉供热气候补偿系统及其实现方法 |
DE102010041222A1 (de) * | 2010-09-22 | 2012-03-22 | Wyatt Technology Europe Gmbh | Vorrichtung Verfahren zur Feldflussfraktionierung |
CN102632088A (zh) * | 2012-03-26 | 2012-08-15 | 唐山钢铁集团微尔自动化有限公司 | 一种带伺服阀补偿的恒轧制力及辊缝综合控制方法 |
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DE202014101518U1 (de) * | 2014-03-31 | 2014-05-30 | Postnova Analytics Gmbh | Vorrichtung für die Feldflussfraktionierung |
CN203964965U (zh) * | 2014-05-29 | 2014-11-26 | 上海梅山钢铁股份有限公司 | 一种宽流量计量装置 |
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JP2008000724A (ja) | 2006-06-26 | 2008-01-10 | Shimadzu Corp | フィールドフローフラクショネーション装置 |
JP2008200566A (ja) | 2007-02-16 | 2008-09-04 | Shimadzu Corp | フィールドフローフラクショネーション装置 |
US20160238571A1 (en) | 2015-02-13 | 2016-08-18 | Postnova Analytics Gmbh | Apparatus for Field-Flow Fractionation |
WO2018056166A1 (ja) | 2016-09-20 | 2018-03-29 | 株式会社島津製作所 | フィールドフローフラクショネーション装置 |
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EP3778031A1 (en) | 2021-02-17 |
US20210138482A1 (en) | 2021-05-13 |
CN111971127A (zh) | 2020-11-20 |
CN111971127B (zh) | 2022-06-24 |
JPWO2019198178A1 (ja) | 2021-02-25 |
EP3778031A4 (en) | 2021-12-22 |
US11819859B2 (en) | 2023-11-21 |
WO2019198178A1 (ja) | 2019-10-17 |
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