JP6999175B2 - Oral composition - Google Patents

Description

The present invention relates to an oral composition having a high bile acid excretion effect.

Conventionally, persimmons are known to have an effect of excreting bile acids, and for example, an agent for promoting excretion of bile acids containing immature persimmon fruits has been proposed (see Patent Document 1).

However, the bile acid excretion promoting agent described in Patent Document 1 is made from immature persimmon fruit (meaning persimmon fruit plucked between flowering and 2 weeks before maturity), and the immature persimmon fruit is dried and dried. The powdered composition is characterized by containing 5.5 to 10% of water and 3.3 to 15% of tannin acid. It was not easy to prepare because of the limitation.
Therefore, there is a demand for a new composition having such a bile acid excretion effect.

Japanese Patent Application Laid-Open No. 2008-06332

An object of the present invention is to provide a new oral composition having a high bile acid excretion effect.

While searching for a composition having a bile acid excretion effect, the present inventors have found that a composition containing charcoal and iron has a high bile acid excretion effect, and have completed the present invention.

That is, the present invention is as follows.
[1] An oral composition comprising charcoal and iron.
[2] The oral composition according to the above [1], which further contains young barley leaves.
[3] The oral composition according to the above [1] or [2], which is used for excretion of bile acid.
[4] The oral composition according to any one of the above [1] to [3], which is used for suppressing fat absorption.
[5] The oral composition according to any one of the above [1] to [4], which is used for suppressing an increase in blood cholesterol.
[6] The oral composition according to any one of the above [1] to [5], which is used for anti-obesity (diet).

The oral composition of the present invention has a high bile acid excretion effect.

In addition, the oral composition of the present invention has a high fat absorption inhibitory effect.
That is, bile acid promotes fat absorption in the intestine, but the oral composition of the present invention can suppress fat absorption in the intestine because it adsorbs to bile acid in the intestine and excretes bile acid.

In addition, the oral composition of the present invention has a high effect of suppressing an increase in blood cholesterol.
That is, bile acids are produced by being catabolized from cholesterol in the liver, and although a part of them is excreted from the body in the intestine, most of them are reabsorbed and returned to the liver. Since it adsorbs to acid and excretes bile acid, it reduces the amount of bile acid that returns from the intestine to the liver, increases the production of bile acid from cholesterol in the liver, and suppresses the rise in blood cholesterol. Can be done.

Furthermore, the oral composition of the present invention has a high anti-obesity effect due to the above-mentioned bile acid excretion effect, fat absorption inhibitory effect, and blood cholesterol elevation inhibitory effect.

It is a figure which shows the amount of bile acid adsorption by the composition of this invention.

The oral composition of the present invention is characterized by containing charcoal and iron. In the oral composition of the present invention, since charcoal and iron are used in combination, bile acids can be effectively adsorbed and excreted. In addition, based on this bile acid excretion effect, it is possible to suppress an increase in blood cholesterol (LDL cholesterol) and fat absorption in the intestine. Therefore, the oral composition of the present invention is useful not only for prevention and / or amelioration of obesity, but also for prevention and / or amelioration of lifestyle-related diseases such as hyperlipidemia, arteriosclerosis, fatty liver, and cardiovascular disease.

The charcoal contained in the oral composition of the present invention is not particularly limited as long as it is edible, and examples thereof include rice husk charcoal, coconut husk charcoal, peach husk charcoal, charcoal, bamboo charcoal, pure charcoal, activated carbon and the like. Can be done. These charcoals are obtained, for example, by burning rice husks, coconut husks, walnut husks, trees such as pine, oak, oak, bamboo, cellulose extracted from these, and synthesized cellulose to carbonize and grind them. be able to. As the charcoal, rice husk charcoal, coconut husk charcoal, charcoal, bamboo charcoal, pure charcoal, and activated charcoal are preferable, and rice husk charcoal, coconut husk charcoal, pine charcoal, bamboo charcoal, and activated charcoal are particularly preferable. Activated carbon is a porous carbon substance activated by carbonizing carbonic substances such as rice husks, coconut husks, wood, and bamboo by heat treatment.

The iron contained in the oral composition of the present invention is not particularly limited as long as it is a compound containing iron, and examples thereof include sodium iron chlorophyllin, iron citrate, and iron pyrophosphate.

The oral composition of the present invention preferably contains young barley leaves in addition to charcoal and iron. By containing young barley leaves, the bile acid excretion effect can be further enhanced. In addition, it can be easily ingested as green juice.

Barley (Hordeum vulgare L.) is a first-year or perennial herb that is native to Central Asia and belongs to the Gramineae family. The young barley leaves used in the oral composition of the present invention are not particularly limited as long as they are usually available, and any young barley leaves such as two-row barley and six-row barley may be used. Moreover, any kind of young barley leaves may be used. The young barley leaves may contain young leaves of barley and may contain other parts such as stems.

In addition, young barley leaves can be used as crushed products, juices, extracts and the like. Examples of the crushed product include powder, granules and the like. The squeezed juice and the extract may be liquid, but can also be used as a paste or a dry powder. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, water (hot water, hot water), ethanol, or hydrous ethanol can be used.

The oral composition of the present invention may contain other green juice materials together with or in place of the young barley leaves. Examples of the green juice material include kale, Angelica keiskei, Kumazasa, and longevity grass.

The oral composition of the present invention can be produced by a known method such as mixing by adding components other than the above, if necessary. Other components include, for example, water-soluble vitamins (vitamins B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P), oil-soluble. Vitamins such as vitamins (vitamins A, D, E, K); minerals such as calcium, magnesium and phosphorus; sulfur-containing compounds contained in taurine, garlic and the like; flavanoids or flavonoids such as hesperidin and quercetin; collagen and the like Examples thereof include proteins; peptides; amino acids; animal fats and oils; vegetable fats and oils; crushed products or extracts of animals and plants.

The oral composition of the present invention has a bile acid excretion effect, a fat absorption inhibitory effect and a blood cholesterol increase inhibitory effect based on the bile acid excretion effect, and further, a bile acid excretion effect, a fat absorption inhibitory effect and a blood cholesterol increase inhibitory effect. It has an anti-obesity (diet) effect based on It can be used as so-called health foods such as recognized functional foods, general foods, food additives, feeds and the like.

The oral composition of the present invention can be used as a composition for suppressing fat absorption used for suppressing fat absorption, and the composition for suppressing fat absorption contains charcoal and iron and is used for suppressing fat absorption. In that respect, the product is not particularly limited as long as it can be distinguished from other products, and for example, fat is absorbed in any of the main body, packaging, instruction manual, and advertising material of the product according to the present invention. The scope of the present invention includes those indicating that they have an inhibitory function. The composition for suppressing fat absorption of the present invention is not limited to the composition in which the components (charcoal and iron) of the combination in the present invention are displayed as active ingredients for suppressing fat absorption on the packaging of the product, for example, effective. It may be one in which the component is not specified.

Specific examples of the composition for suppressing fat absorption of the present invention include pharmaceuticals (including quasi-drugs) and so-called health foods. In so-called health foods, "suppressing fat absorption" and "fat absorption" Examples of those displaying "reduce absorption", "for those who want to suppress fat absorption", etc. can be exemplified.

The oral composition of the present invention can be used as a composition for suppressing an increase in blood cholesterol used for suppressing an increase in blood cholesterol, and the composition for suppressing an increase in blood cholesterol contains charcoal and iron. The product is not particularly limited as long as it can be distinguished from other products in that it is used for suppressing the increase in blood cholesterol. The scope of the present invention includes any of the advertisements indicating that the product has a function of suppressing the increase in blood cholesterol. The composition for suppressing the increase in blood cholesterol of the present invention is not limited to the composition in which the components (charcoal and iron) of the combination in the present invention are displayed as the active ingredient for suppressing the increase in blood cholesterol on the packaging of the product. For example, the active ingredient may not be specified.

Specific examples of the composition for suppressing the increase in blood cholesterol of the present invention include pharmaceuticals (including non-medicinal products) and so-called health foods. In so-called health foods, "suppressing the increase in cholesterol" and " Examples of those displaying "for those who are concerned about cholesterol", "suppressing the absorption of cholesterol", "for those with high cholesterol", etc. can be exemplified.

Further, the oral composition of the present invention can be used as an anti-obesity composition used for anti-obesity, and the anti-obesity composition contains charcoal and iron and is used for anti-obesity. In the above, the product is not particularly limited as long as it can be distinguished from other products, and for example, any of the main body, packaging, instruction manual, and advertisement of the product according to the present invention has an anti-obesity effect. Those indicating that they have a function are included in the scope of the present invention. The composition for anti-obesity of the present invention is not limited to the one in which the components (charcoal and iron) of the combination in the present invention are displayed as the active ingredients of anti-obesity on the packaging of the product, for example. It may not be specified.

Specific examples of the anti-obesity composition of the present invention include pharmaceuticals (including non-medicinal products) and so-called health foods. In so-called health foods, "for those who are concerned about body weight" and " "For those who are overweight", "For those who are concerned about body weight (BMI)", "Reduce weight and abdominal fat (visceral fat and subcutaneous fat)", "Reduce waist circumference system", etc. It can be exemplified.

The form of the oral composition of the present invention includes, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. The shape, gel shape, chewable shape, stick shape and the like can be mentioned. Among these, tablet-like, capsule-like, powder-like, granular, and liquid forms are particularly preferable. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., powders for dissolving in water (hot water), milk, fruit juice, green juice, etc., and food additives. It can be exemplified. These are preferable in that they are easy to drink at the time of meals and can enhance the palatability.

The content of charcoal and iron (two components of the present invention) in the oral composition of the present invention may be appropriately contained within the range in which the effect is exhibited.

Generally, when the oral composition of the present invention is a drug such as a tablet or a supplement, the two components of the present invention are contained in an amount of 0.01 to 100% by mass of the entire composition in terms of dry mass. It is preferable that it is contained in an amount of 0.03 to 85% by mass, more preferably 0.05 to 70% by mass.

When the oral composition of the present invention is a packaged beverage, the two components of the present invention are preferably contained in an amount of 0.0001 to 50% by mass, preferably 0.0005 to 40% by mass, based on the total amount of the composition. It is more preferable that the content is 0.001 to 30% by mass, and it is more preferable that the content is 0.001 to 30% by mass.

When the oral composition of the present invention is a powdered beverage, it is preferable that the two components of the present invention are contained in an amount of 0.0001 to 80% by mass, preferably 0.0005 to 80% by mass, in terms of dry mass. It is more preferably contained in an amount of 70% by mass, and further preferably contained in an amount of 0.001 to 60% by mass.

Furthermore, when the oral composition of the present invention contains charcoal, iron and young barley leaves, and in the case of pharmaceuticals and supplements such as tablets, the three components of the present invention are 0.05 to 100 in terms of dry mass of the entire composition. It is preferably contained in an amount of 0.075 to 85% by mass, more preferably 0.1 to 70% by mass, and even more preferably 0.1 to 70% by mass.

When the oral composition of the present invention is a packaged beverage, the three components of the present invention are preferably contained in an amount of 0.0001 to 50% by mass, preferably 0.0005 to 40% by mass, based on the total amount of the composition. It is more preferable that the content is 0.001 to 30% by mass, and it is more preferable that the content is 0.001 to 30% by mass.

When the oral composition of the present invention is a powdered beverage, it is preferable that the three components of the present invention are contained in an amount of 0.0005 to 90% by mass, preferably 0.001 to 90% by mass, in terms of dry mass. It is more preferably contained in an amount of 80% by mass, and further preferably contained in an amount of 0.005 to 70% by mass.

In order to exert the effect of the present invention more effectively, it is preferable that the three components of the present invention are contained in an amount of 0.1% by mass or more in terms of dry mass, and 1% by mass or more. It is more preferable that it is contained in an amount of 5% by mass or more, and it is particularly preferable that it is contained in an amount of 10% by mass or more.

The intake amount of the oral composition of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, the intake amount of the two components of the present invention per day should be 5 mg / day or more. It is preferable to take it at 10 mg / day or more, and it is more preferable to take it at 20 mg / day or more. The oral composition of the present invention can be contained in one container or divided into a plurality of containers, for example, 2 to 3 so that the daily intake becomes the above-mentioned intake, and can be contained as one day's worth.

The compounding mass ratio of charcoal and iron is preferably 0.01 to 2000: 1, more preferably 0.05 to 1000: 1, and 0.1 to 100: 1 in terms of dry mass. It is more preferable to have. The blending mass ratio of charcoal and iron to young barley leaves is preferably in the range of 0.01 to 2000: 1, preferably in the range of 0.05 to 1000: 1 in terms of dry mass. More preferably, it is in the range of 0.1 to 100: 1.

Hereinafter, the present invention will be described based on examples.

<Bile acid adsorption capacity test>
(Preparation of test substance)
Each raw material (charcoal, iron, barley young leaf powder) was blended as shown in Table 1, and the test substances of Comparative Examples 1 to 3 and Examples 1 and 2 were prepared.

(Sample preparation)
For 6 mL of postprandial artificial intestinal juice warmed to 37 ° C (FeSSIF: 100 mM sodium taurocholic acid 15 mL, lecithin 284.3 mg, 1M acetic acid 14.4 mL, 1 M sodium hydroxide 10.1 mL, 1 M sodium chloride 17.3 mL, pH 5.0) The test substance was added at a concentration of 0.05% (W / V) and vortexed. Then, it was warmed in a constant temperature bath at 37 ° C. for 30 minutes while shaking at 100 rpm. In addition, as a control, 6 mL of postprandial artificial intestinal juice was warmed in a constant temperature bath at 37 ° C. for 30 minutes while shaking at 100 rpm.

After warming for 30 minutes, it was centrifuged at 2000 rpm for 10 minutes.

3 mL of the supernatant was taken so as not to absorb the precipitate (test substance), an equal amount of ethyl acetate was added to the supernatant, and the mixture was vortexed for 30 seconds. After vortexing, it was centrifuged at 2000 rpm for 10 minutes.

1.5 mL of the upper layer of the liquid was transferred to a new centrifuge tube and dried using a centrifugal evaporator.

600 μL of 2-propanol was added to the dry matter and vortexed for 10 seconds. After vortexing, it was suspended by ultrasound for 10 minutes.

Then, it was centrifuged at 800 rpm for 3 minutes, and the supernatant was collected in a 1.5 mL tube to obtain a sample.

(Measurement of bile acid concentration)
Total bile acid test Using Wako, the bile acid concentration in the sample was measured by the following method.

62.5 μL of the enzyme solution was added to 25 μL of the sample, mixed well, and then heated at 37 ° C. for 10 minutes. Then, 62.5 μL of the reaction terminator was added, mixed well, and the absorbance at 560 nm was measured. Further, 25 μL of the sample to which 62.5 μL of the blind enzyme solution was added was also reacted in the same manner to prepare a blank of the sample.

62.5 μL of the enzyme solution was added to 25 μL of the 2-propanol solution in which the concentration of glycocholic acid was adjusted to 10, 20, 30, 40, and 50 μmol / L, and the mixture was well mixed and then heated at 37 ° C. for 10 minutes. Then, 62.5 μL of the reaction terminator was added, mixed well, and the absorbance at 560 nm was measured to prepare a calibration curve. Further, 25 μL of a 2-propanol solution having an adjusted glycocholic acid concentration of 10 μmol / L to which 62.5 μL of an inactive blind enzyme solution was added was also reacted in the same manner, and the solution was used as a blank of the standard solution. did.

The bile acid concentration (μmol / L) of the sample was obtained from the calibration curve, converted into a relative value (%) when the control was set to 100%, and then the adsorption amount from the relative value was calculated.

The results are shown in FIG.

As shown in FIG. 1, it was found that the combination of charcoal and iron improved the amount of bile acid adsorbed as compared with the addition of charcoal and iron alone. Further, as shown in FIG. 1, it was found that the adsorbability of bile acid was synergistically increased by combining charcoal and iron with young barley leaves.

[Manufacturing Example 1] (Manufacturing of tablets)
A tablet consisting of the following ingredients was produced. The obtained composition was expected to have a bile acid adsorption effect.

Charcoal powder (derived from bamboo) 0.08%
Iron chlorophyll 0.0001%
Barley young leaf powder 0.04%
Vitamin 5%
Silicon dioxide 6%
Maltose 55.5799%
Reduced maltose 0.3%
Droxypropyl Cellulose 15%
Calcium stearate 3%
Hydroxypropyl Methyl Cellulose 15%

[Production Example 2] (Production of granules)
A granule composed of the following components was produced. The obtained composition was expected to have a bile acid adsorption effect.

Charcoal powder (derived from palm shell) 70%
Iron chlorophyll 20%
Barley young leaf powder 2.25%
Calcium stearate 1.5%
Dolomite 6.25%
[Manufacturing Example 3] (Manufacturing of powdered beverage)
A powdered beverage consisting of the following ingredients was produced. The obtained composition was expected to have a bile acid adsorption effect.

Charcoal powder (derived from rice husk) 0.02%
Iron chlorophyll 0.16%
Barley young leaf extract powder 1%
Vitamin 3%
Reduced maltose 35.82%
Indigestible dextrin 60%

The oral composition of the present invention is industrially useful because it has a high bile acid excretion effect.

Claims (4)

An oral composition for suppressing an increase in blood cholesterol , which comprises charcoal and iron. An oral composition for suppressing fat absorption , which comprises charcoal and iron. An oral composition for suppressing an increase in blood cholesterol , which comprises charcoal, iron and young barley leaves. An oral composition for suppressing fat absorption , which comprises charcoal, iron and young barley leaves.

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