JP6979687B2 - 幹細胞の凝集塊の集団の調製方法 - Google Patents

幹細胞の凝集塊の集団の調製方法 Download PDF

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JP6979687B2
JP6979687B2 JP2017559247A JP2017559247A JP6979687B2 JP 6979687 B2 JP6979687 B2 JP 6979687B2 JP 2017559247 A JP2017559247 A JP 2017559247A JP 2017559247 A JP2017559247 A JP 2017559247A JP 6979687 B2 JP6979687 B2 JP 6979687B2
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pluripotent stem
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JPWO2017115865A1 (ja
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剛士 田邊
健太 須藤
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I Peace Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2535/00—Supports or coatings for cell culture characterised by topography

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  • Health & Medical Sciences (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2017559247A 2015-12-29 2016-12-28 幹細胞の凝集塊の集団の調製方法 Active JP6979687B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562272524P 2015-12-29 2015-12-29
US62/272,524 2015-12-29
PCT/JP2016/089185 WO2017115865A1 (fr) 2015-12-29 2016-12-28 Procédé de préparation d'une population de sphéroïdes de cellules souches

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JPWO2017115865A1 JPWO2017115865A1 (ja) 2018-11-08
JP6979687B2 true JP6979687B2 (ja) 2021-12-15

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US (1) US20190002834A1 (fr)
JP (1) JP6979687B2 (fr)
CN (1) CN108699518A (fr)
WO (1) WO2017115865A1 (fr)

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JP2019062832A (ja) * 2017-10-02 2019-04-25 国立大学法人京都大学 スフェロイドを製造するためのデバイス、スフェロイドの製造及び回収方法
EP3730601A4 (fr) * 2018-01-31 2021-03-10 Yamaha Hatsudoki Kabushiki Kaisha Procédé et dispositif de déplacement de matière biologique
WO2019153006A1 (fr) * 2018-02-05 2019-08-08 Brown University Empilement et manipulation de microtissus guidés par entonnoir
JP7271903B2 (ja) * 2018-10-20 2023-05-12 東洋製罐グループホールディングス株式会社 スフェア培養部材、培養容器、穴開き部材の加工方法、及び洗浄容器
CN109913373A (zh) * 2019-04-18 2019-06-21 深圳华源再生医学有限公司 一种可重复使用3d打印干细胞培养与分化皿系统
WO2020255546A1 (fr) * 2019-06-20 2020-12-24 Jsr株式会社 Procédé et dispositif de fabrication de groupe d'amas cellulaire
WO2021039882A1 (fr) * 2019-08-28 2021-03-04 学校法人東海大学 Procédé de culture d'une population cellulaire contenant des cellules souches/progénitrices positives tie2 à l'aide d'un substrat de culture et utilisation correspondante
US20220010282A1 (en) * 2020-06-16 2022-01-13 The Broad Institute, Inc. Three-dimensional cell culture, devices, and use thereof
EP4180515A1 (fr) * 2020-08-18 2023-05-17 Tosoh Corporation Procédé pour induire la différenciation de cellules souches pluripotentes en cellules ectodermiques, mésodermiques et endodermiques
WO2023053220A1 (fr) * 2021-09-28 2023-04-06 公益財団法人京都大学ｉＰＳ細胞研究財団 Procédé de production de cellules souches pluripotentes

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EP2537921A1 (fr) * 2006-03-31 2012-12-26 Daiichi Sankyo Company, Limited Nouveau procédé de culture cellulaire et procédés de production et de collecte de masses cellulaires utilisant celui-ci
US8372642B2 (en) * 2009-02-27 2013-02-12 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
CN102947710B (zh) * 2010-01-28 2015-01-14 3D生物母体公司 悬滴装置、系统和/或方法
DK2878664T3 (en) * 2012-07-24 2018-11-05 Nissan Chemical Corp CULTURE MEDIUM COMPOSITION AND PROCEDURE FOR CULTIVATING CELLS OR TISSUE USING THE COMPOSITION
JP6429280B2 (ja) * 2013-05-14 2018-11-28 国立大学法人京都大学 効率的な心筋細胞の誘導方法
EP3048169B1 (fr) * 2013-09-04 2019-07-10 Otsuka Pharmaceutical Factory, Inc. Procédé de préparation de cellules souches pluripotentes
EP3045892A4 (fr) * 2013-09-09 2016-11-02 Riken Dispositif d'analyse de gaz et procédé d'analyse de gaz
WO2015129263A1 (fr) * 2014-02-25 2015-09-03 株式会社クラレ Dispositif de fabrication de sphéroïdes, et procédé de récupération et procédé de fabrication de sphéroïdes

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US20190002834A1 (en) 2019-01-03
WO2017115865A1 (fr) 2017-07-06
CN108699518A (zh) 2018-10-23
JPWO2017115865A1 (ja) 2018-11-08

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