JP6876283B2 - Concentration measurement system - Google Patents

Description

The present invention relates to a concentration measuring system and a concentration measuring method.

Conventionally, when trying to analyze substances in soil, residual pesticides, and even substances, elution is performed, color development is performed with the necessary chemicals, and a colorimetric meter is applied to compare the concentration with the calibration curve prepared in advance. Is the normal working procedure.
However, there is a problem that the analysis work cannot be performed unless the place where the colorimeter is provided and the calibration curve is prepared.

In order to solve such a problem, for example, in Patent Document 1, a substance dissolved in a solution is colored with a color-developing agent, and the concentration of the substance is measured by camera photography with three stimulus values (values of R, G, B). ), Describes the invention obtained by the calibration curve prepared in advance. In this document, factory wastewater, rivers, cement, etc. are assumed to be measured.

Further, Patent Document 2 describes continuous colorimetric analysis of sample concentration in a solution. It is an invention in which a solution is flowed on a slope, a chemical for color development is added, the state of color development is photographed with a television camera, the data is converted into RGB numerical values, and the concentration is obtained by comparing with known data.

JP-A-2007-33036 Japanese Patent Application Laid-Open No. 2-208543

In the method described in Patent Document 1, it is necessary to prepare a calibration curve of the target substance in advance, and under conditions where the light source is not constant, the concentration of the measured solution varies widely, and further, a petri dish is used. Since it is used for measurement, the bottom surface of the petri dish becomes dark at the time of shooting, which causes a problem such as an error in measurement. Further, when shooting from the front, there is a problem that the measurement result varies widely because the light source is blocked by the imaging means. Further, in Patent Document 2, preparation is complicated because a special device such as a device for flowing a solution or an image processing device is required. In each case, it has been a problem that the concentration of the target substance cannot be known instantly when measured and the measured concentration cannot be shared.

As a result of diligent studies to solve the above problems, the present inventor, if there is a correlation between the density of the sample and the color information, the color of the measurement sample is based on the color information of the substance whose concentration is known. I thought that the concentration could be quantified from the information.

That is, in the present invention, the concentration is quantified based on the color information of the sample whose concentration is known as a chemical state, and a calibration curve is created from the color information of the sample whose concentration is known, and the correlation with the color information of the measurement sample is used. Ru concentration measurement system der to quantify the concentration of a single or a mixture. In this concentration measurement system, an optical cell having an optical path length of 1 mm or more and 10 mm or less and transmitting light having a wavelength of 220 nm or more and 800 nm or less, which can store and hold at least the object to be measured, which is a means for holding the object to be measured, and the optical cell are fixed. a fixture, and an imaging device, light source, the closer to the imaging unit of the lower side than the position or the object to be measured away from the imaging means of the upper side than the object to be measured housed in an optical cell location a reference plate serving as a reference including an indication symbol indicating the position of at least two different reference colored portions and the reference colored portions rather location in, the placed spaced from the rear measured object stored in the optical cell to be A measuring plate including a measuring unit acquired as an image by the imaging means at the same time as the object to be measured housed in the optical cell via the measuring object is provided, and the position of the imaging means is indicated by the instruction symbol. An image processing means for simultaneously acquiring at least two different reference coloring units and the measuring unit as images and processing the acquired images as the color information, and an operation for calculating the density based on the color information. It is a concentration measurement system including means, data storage display means, and data transmission means.

The concentration measuring system of the present invention is characterized in that the object to be measured is a liquid.

The concentration measuring system of the present invention is characterized in that the object holding means is an optical cell that transmits light having an optical path length of 1 mm or more and 10 mm or less and a wavelength of 220 nm or more and 800 nm or less.

In the concentration measurement system of the present invention, the first reference coloring portion provided on the reference plate is subjected to the first colorant containing 0% or more and less than 50% of carbon black in order to give the first color information. of a reference colored portion, when 255 dividing the first color information of the first reference colored portion is measured by the image pickup means as a numerical value, R (sometimes referred to as red) 100 and 255 in , G (sometimes referred to as green) at 100 to 255, B (sometimes referred to as blue) with and from 100 to 255, the reference color portions of the second is to provide the second color information Is a second reference coloring portion to which a colorant containing 50% or more and less than 95% of carbon black and having a content of 10% or more as compared with the content of carbon black that gives the first color information is applied, and is an imaging means. When the color information measured by is divided into 255 as numerical values, it is characterized in that R is 1 or more and 99 or less, G is 1 or more and 99 or less, and B is 1 or more and 99 or less. It is particularly preferable that the reference plate is arranged parallel to the light source.

When three or more color information is used for this reference plate , the amount of carbon black used to give the third or more color information is applied to the first reference coloring portion and the second reference coloring portion. It is characterized in that there is a difference in content of 10% or more as compared with the amount of each carbon black used for the purpose.

In the concentration measuring system of the present invention, the reference plate has an indicator symbol indicating the position of the reference colored portion. The presence of this indicator symbol is preferable because it eliminates the uncertainty of the position when a colorant containing no carbon black is applied to the reference coloring portion and can acquire accurate color information.

A concentration measurement system of the present invention, wherein the measuring unit provided in the measurement plate, a measuring unit which has been subjected to a coloring agent comprising carbon black less than 95% 0% in order to provide color information of the measuring portion the imaging When the color information of the measuring unit measured by the means is divided into 255 as numerical values, it is characterized in that R is 1 or more and 255 or less, G is 1 or more and 255 or less, and B is 1 or more and 255 or less.

The concentration measuring system of the present invention is characterized in that the light source is an LED or a fluorescent lamp.

A concentration measurement system of the present invention, the imaging means, at least two different said reference colored portion, the digital camera can be acquired at the same time a measurement unit, as an image, any of the media tablet device having a CCD camera, an image acquisition function Is selected from.

A concentration measurement system of the present invention, the image processing means, the color information obtained for each image portion from the obtained said two different reference color portions of the image by the image pickup means every the color information R 1 A means for converting to any of more than 255, more than 1 and more than 255 for G, and 1 or more and less than 255 for B and using this RGB numerical information as reference color information, and at least two different known densities stored in each optical cell. substance with R color information obtained for each image region from an image of the measurement unit provided in the measurement plate placed spaced rearwardly from these materials housed in the optical cell for each the color information A means for converting 1 or more and 255 or less, G for 1 or more and 255 or less, and B for 1 or more and 255 or less and using this RGB numerical information as reference density color information, and at least one unknown point stored in the optical cell. The image of the measurement unit provided on the object to be measured of the substance of concentration and the measurement plate placed behind the object to be measured and stored in the optical cell and the image of the reference coloring area are simultaneously acquired and respectively. Based on the image of, the color information obtained from the image of this measuring unit is converted into any of 1 or more and 255 or less for R, 1 or more and 255 or less for G, and 1 or more and 255 or less for B, and this RGB numerical information is converted. means for the uncorrected concentration color information, 255 or less 1 or more R to image for each site of measurement of the reference colored portion which is acquired simultaneously with the image of the measuring portion, one or more 255 or less in G, 1 in B It is characterized in that it comprises, means for the 255 following converts any numerical information measurement reference color information in the RGB or more.

The concentration measurement system of the present invention, the calculating means, using said reference color information, and the reference density color information, and the pre-correction density color information, wherein the measurement reference color information, and the known concentration, and, and the reference density color information, and the known concentration gave respective reference density color information, to create a concentration measurement calibration curve for the concentration of. Further the step of creating from the slope of the concentration determination calibration curve (Equation 1), to create a corrected calibration curve from said measurement reference color information and the reference color information from a straight line the slope of the correction calibration curve (Equation The step of creating 2), the step of calculating the measured density color information by the pre-correction density color information and (Equation 3), and the calculation of the concentration of the substance having the unknown density by the measurement density color information and (Equation 4). a step of a calculation means for performing. These arithmetic means can be performed programmatically or manually.
(Equation 1) Known density = standard density color information x α
(Equation 2) β = Reference color information ÷ Measurement reference color information (Equation 3) Measurement density color information = β × Pre-correction density color information (Equation 4) Density = α × Measurement density color information
Here, α indicates a known density with respect to the reference density color information. β indicates the measured density color information with respect to the density color information before correction.

A concentration measuring system of the present invention, the data storage means the reference color information, the reference density color information, the uncorrected concentration color information, the measurement reference color information, the known concentration, the concentration measurement calibration curve, the correction calibration curve, the measured concentration color information, the concentration of the substance of the unknown concentration, the (formula 1), the equation (2), wherein the concentration measurement system that stores (equation 3) and the (equation 4).

The concentration measuring system of the present invention may also be provided with data transmission means. This data transmission means is a means capable of selecting and communicating from any of e-mail via the Internet, transmission of electronic data and transmission of electronic data by wire, data transmission via a recording medium of electronic data, and infrared communication.

In the concentration measurement system of the present invention, the measurement plate is placed behind the liquid contained in the optical cell having light transmission in the visible light region containing the substances having at least two different known concentrations. The measurement part side of the measuring plate placed at a distance behind the liquid contained in the optical cell having light transmission in the visible light region of the substance of unknown concentration at the part side and at least one point, and this of the optical cell. The distance between the measuring plate and the surface facing it is 1 mm or more and 20 mm or less.

A concentration measurement system of the present invention, the front-rear direction of the angle is the angle minus with respect to the vertical direction of the optical cell (- and sometimes referred) (sometimes referred to as °) 5 degrees angle plus (+ and also referred there) is 5 degrees or less, the angle between the measuring portion which is provided the optical cell and the measuring plate is less than -5 degrees angle of 50 degrees.

In the density measurement system of the present invention , the angle between the center of the optical axis of the light source having the center of gravity of the reference colored portion and the surface to be measured of the measurement portion acquired as the image as the apex and the imaging means is an angle of 1 degree or more. The angle is 135 degrees or less.
To explain this positional relationship in a more understandable manner, in the density measurement system of the present invention, the midpoint of the line connecting the centers of gravity of the reference colored portion and the planes giving the color information of the measuring portion, and the center of the light source. When the line connecting the above with a straight line is used as the center line of the optical axis of the light source, the angle formed by the center line of the optical axis and the line connecting the imaging means and the midpoint is an angle of 1 degree or more. It is 135 degrees or less.

In the density measurement system of the present invention, the area for giving color information of the reference coloring portion and the measuring portion ^{is 1 mm 2} or more and 500 mm ² or less, and the measured shapes are a circle, an ellipse, a triangle, a quadrangle, a hexagon, and an octagon. It is one of the choices from.

The concentration of the substance to be measured is less than or equal to the known concentration. The concentration can be displayed in parts per 100, and when using a sample whose accurate concentration is difficult to determine due to foreign matter, etc., the user can arbitrarily determine the concentration and use it. it can.

As for the substance whose density is to be measured, it is sufficient that the color information obtained by the imaging means correlates with the density. Specifically, iron, manganese, magnesium, phosphorus, calcium, potassium, fluorine, hydrogen, chlorine, hexavalent chromium, nickel, aluminum, silver, gold, copper, boron, molybdenum, palladium, zinc, nitrate ion, ammonium ion. , Hydrogen ion, hydroxide ion, indoxyl, ascorbic acid, rot plant, iodine, lycopene , iron, manganese, magnesium, phosphorus, calcium, potassium, hydrogen, chlorine, nickel, aluminum, copper, boron, molybdenum, palladium, Zinc, nitrogen oxides, or complexes formed with ascorbic acid, iodine, methanephetamine and derivatives thereof, cholineesterase and indoxyl acetate or 5-blomo-6-chloro-3-indoxyl butylate or 5-blomo-6-chloro- From substances such as Escherichia coli, chlorophyll, and yeast that inhibit the action of cholinesterase and inhibit this color development when it reacts with any one of 3-indoxyl capsule, 5-blomo-4-chloro-3-indoxyl palmite and develops color. Be selected.

The concentration measuring system of the present invention can be used as a soil analyzer for measuring nutrients contained in soil.

When the concentration measurement system of the present invention is used, if it is used as a soil analyzer, soil management and evaluation in the agricultural field can be simplified. Large-scale farmers who own multiple fields can manage the soil in each field. In addition, quality control and evaluation in the food field can be simplified, and the presence or absence of dangerous substances such as chemicals can be easily inspected.

It is a schematic block diagram (side view) of the concentration measurement system of this invention. It is a schematic block diagram (partial front view) of the concentration measurement system of this invention. It is a figure of the process carried out by the image processing means of the density measurement system of this invention. It is a flowchart of the process carried out by the calculation means of the concentration measurement system of this invention. It is a figure of the correction calibration curve. It is a figure of the calibration curve for concentration measurement. It is a photograph of a part of the soil analyzer using the concentration measurement system created in Example 1 of the present invention. In this photograph, the positional relationship between the imaging means and the optical cell or the like is different from the position shown in FIG. 1 for measuring the density. This is a part of the output screen of the program incorporating the concentration measurement system created in Example 1 of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the drawings.

In the concentration measuring system 1 of the present invention, the object to be measured 5 is a liquid. The object 5 to be measured is placed in an optical cell 3 that transmits light having an optical path length of 1 mm or more and 10 mm or less and a wavelength of 220 nm or more and 800 nm or less, and the optical cell 3 is placed on a fixing jig 7 to perform suitable analysis.

Reference plate 4A having a reference colored part used in a concentration measuring system 1 of the present invention, the housed in the position or the optical cell 3 away from the imaging means 8A above side than the object to be measured 5 housed in the optical cell 3 location from the measurement object 5 at a position close to the imaging means 8A under side rather. The reference plate 4A is a reference plate including a reference reference coloring portion including at least two different reference coloring portions 4. When two different color information is used, a colorant containing 0% or more and less than 50% of carbon black is applied as a colorant that gives the first color information. When this is measured by the image pickup means 8 A and divided into 255 numerical values by the image processing means of FIGS. 3 and 4 , R is 100 or more and 255 or less, G is 100 or more and 255 or less, and B is 100 or more and 255 or less. The content of carbon black as a colorant that gives the second color information to the reference plate 4A provided with the reference coloring portion is 50% or more and less than 95%, and is 10% or more as compared with the carbon black content that gives the first color information. Apply a colorant that is. When this second color information is divided into 255 as numerical values, a reference plate 4A having a reference coloring portion having R of 1 or more and 99 or less, G of 1 or more and 99 or less, and B of 1 or more and 99 or less can be used. The number of reference coloring portions 4 of the reference plate 4A provided with the reference coloring portion 4 is not particularly limited, but 2 points or more and less than 21 points are preferably used, 5 points or more and 15 points or less are more preferable, and 6 points or more and 8 points or less are used. It is more preferably used. There is no particular problem in measurement even if the number of points is 21 or more, but the production of the reference plate 4A provided with this reference coloring portion tends to be complicated. Further, when two or more points of these reference coloring portions 4 are used, the quantified color information of each is preferably used when they are separated by 10 or more in each of R, G, and B.

Provided in the reference plate, the designator 41 indicative of the position of the reference colored portion may be used symbols such as points and lines. Using a plurality of the indication symbol 41 to determine the position of any of the reference colored portion 4 therebetween, also to be possible to obtain the color information, determined distance criterion in the instruction symbol 41 from the instruction symbol 41 using singular Color information can also be obtained by reading the coloring unit 4.

The measuring plate 4B provided with a measuring unit which is placed at a distance behind the object to be measured 5 housed in the optical cell 3 and is simultaneously acquired as an image through the object 5 to be measured uses carbon black as a colorant which gives color information. When a measuring unit provided with a colorant containing% or more and less than 95% is provided and the color information measured by the imaging means 8A is divided into 255 as numerical values, R is 1 or more and 255 or less, G is 1 or more and 255 or less, and B is 1. It is preferably used in the above 255 or less. As described in Examples described later, when measuring the potassium concentration in soil and solution, the potassium concentration can be suitably measured by applying a colorant containing 80% or more and 95% or less of carbon black to the measuring portion. ..

In the concentration measurement system 1 of the present invention, an LED (Light emitting diode) or a fluorescent lamp can be preferably used as the light source 2. Further, when there is no light source that emits light by using electricity, natural light such as sunlight can be preferably used. When an LED is selected as the light source 2, it is sufficient that a calibration curve (FIG. 6) for measuring the concentration of the object to be measured 5 can be formed for the wavelength of the LED. In the case of an LED, it is preferably used to develop the light of each of R, G, and B. The wavelength of R light is preferably longer than 600 nm and preferably 800 nm or less, more preferably 610 nm or more and 700 nm or less, and more preferably 620 nm or more and 650 nm. A light source having a wavelength higher than this can be used, but the light source tends to be expensive. The wavelength of G light is preferably 495 nm or more and 600 nm or less, more preferably 500 nm or more and 550 nm or less, and more preferably 520 nm or more and 540 nm. The wavelength of the light of B is preferably 300 nm or more and 495 nm or less, more preferably 330 nm or more and 480 nm or less, and more preferably 350 nm or more and 475 nm. If the wavelength is 300 nm or less, the light source tends to be expensive. As the fluorescent lamp, one that emits light having a wavelength of 300 nm or more and 800 nm or less can be preferably used. Further, these wavelengths can be arbitrarily selected depending on the measurement target.

The image pickup means 8A for acquiring an image used in the density measurement system 1 of the present invention can be selected from any of an image pickup device 8 such as a camera, a digital camera, a CCD camera, and a media tablet terminal having the image pickup means 8A. .. It is also preferably used for a digital camera mounted on a personal computer. If the image pickup device 8 of a media tablet terminal or the like has a function of acquiring position information, a function of recording the date and time, and a function of acquiring the weather and temperature, the position and place where the density is measured, the date and time, the weather and temperature, which will be described later, Can be saved at the same time, and data can be transmitted by the data transmission means described later. In addition, information related to the object to be measured 5 can also be stored as electronic data and transmitted. It can be preferably used when the image pickup apparatus 8 has a timer function. This is particularly preferable when a certain elapsed time is required when the measurement target is colored by the coloring liquid.

Image processing means used in the concentration measuring system 1 of the present invention is Ru can also be implemented as a program in the imaging device 8 having the imaging unit 8A.
This image processing means obtains the color information acquired for each image portion from the image of the reference coloring unit 4A as a reference including the two different color information 4 obtained by the imaging means 8A, and the color information is 1 for each color information. It includes means for converting to any one of 25 or more, 255 or less in G, 1 or more and 255 or less in B, and 1 or more and 255 or less in B, and using this RGB numerical information as reference color information.
Further, this image processing means obtains each image from an image of a liquid stored in an optical cell 3 of at least two different known concentrations of a substance and a measuring unit provided on a measuring plate placed behind the liquid. The color information acquired for each part is converted into any of 1 or more and 255 or less for R, 1 or more and 255 or less for G, and 1 or more and 255 or less for B for each color information, and this RGB numerical information is used as the reference density color information. Including means .
Furthermore the image processing means includes an image of the measuring unit provided in the measurement plate is placed apart from the liquid accommodated in the optical cell 3 substances unknown concentration of at least one point than those at the rear, the reference The image of the colored part and the image of the colored part are acquired at the same time , and the color information obtained from the image of the measuring part based on each image is 1 or more and 255 or less for R, 1 or more and 255 or less for G, and 1 or more and 255 or less for B. means to convert to any numerical information before correction density color information of the RGB of 1 R image color information of the reference colored portion image to have been acquired at the same time of the measurement unit for each site of measurement It includes at least a means for converting the numerical value information of RGB into any one of 255 or more, 1 or more and 255 or less for G, and 1 or more and 255 or less for B, and using this RGB numerical information as the reference color information for measurement.

Computing means concentration measurement system 1 of the present invention can also be implemented as a program in the imaging device 8 having the imaging unit 8A, and the reference color information obtained by the image processing means, and the reference density color information, wherein and uncorrected density color information, wherein the measurement reference color information, the known concentration and a reference, calibrator concentration measurement to the concentration of said known concentration gave the reference density color information of each said reference density color information create a line, from the slope of the concentration determination calibration curve the steps of creating (equation 1), to create a corrected calibration curve from said reference color information and the measurement reference color information in the linear of the correction calibration curve a step of creating from the slope (equation 2), the uncorrected concentration color information and calculating a measured density color information by (equation 3), and the measured density color information to the concentration of a substance of unknown concentration (equation 4 ) , And a calculation means for carrying out.
(Equation 1) Density = Reference density Color information x α
(Equation 2) β = Reference color information ÷ Measurement reference color information (Equation 3) Measurement density color information = β × Pre-correction density color information (Equation 4) Density = α × Measurement density color information

In the concentration measurement system 1 of the present invention (Equation 1), (Equation 2), (Equation 3), and (Equation 4), the phase relationship when the intercept is added to the calibration curve as compared with the case where the intercept is not added to the calibration curve. Of course, if the number is high, the intercept can be used.

Data storage and display unit of the system of the present invention can also be implemented as a program in the imaging device 8 having the imaging means 8A, the reference color information, the reference density color information, the uncorrected concentration color information, the measurement reference color information, the known concentration, the concentration measurement calibration curve, the correction calibration curve, the measured concentration color information, the concentration of the substance of unknown concentration, the (formula 1), the equation (2), the equation (3) And the above (Equation 4) can be saved and optionally displayed on a screen or the like as shown in FIGS. 7 and 8. Further, as described above, the position and place where the concentration was measured, the date and time, the weather and the temperature can be saved at the same time. In addition, information related to the measurement target can be preferably stored as electronic data.

The concentration measurement system 1 of the present invention may also be provided with data transmission means. As this data transmission means , at least electronic mail via the Internet, transmission of electronic data, transmission of electronic data by wire, and data transmission via a recording medium of electronic data can be used.

In the concentration measurement system 1 of the present invention, a liquid (object 5 to be measured) containing a substance having a known concentration at least two different points is placed in an optical cell 3, and a liquid containing the substance having a known concentration contained in the optical cell 3 is placed. the measurement plate 4B placed spaced rearwardly and unknown concentration placed in the optical cell 3 the liquid between the optical cell 3 of the measuring plate 4B placed spaced to the rear of (object to be measured 5) The distance between the opposing surfaces is preferably 1 mm or more and 20 mm or less, and the distance between the opposing surfaces of the measuring plate 4B with the optical cell 3 is preferably 5 mm or more and 18 mm or less.
It is more preferable that the distance between the surface of the measuring plate 4B and the optical cell 3 facing each other is 6 mm or more and 17 mm or less. The distance opposing surfaces of the optical cell 3 measured plate 4B is too close shadow of the optical cell 3 moves to the measurement plate 4B, the phase relationship between the concentration measurement calibration curve created from a known concentration and the reference concentration color information The number may decrease and the measured density may differ from the actual one, and if it is too far away, it may become out of focus when imaging.

The density measuring system 1 of the present invention, the longitudinal direction of the angle of the optical cell 3 are preferably used in the following angle minus 5 degrees angle plus 5 degrees with respect to the vertical direction. If the angle of the optical cell is tilted more than plus or minus 5 degrees with respect to the vertical direction, the object 5 inside tends to spill easily. Further, when taking an image, light from a light source or other outside may be reflected on the surface of the optical cell 3 and cause an error in the measurement result. If the correlation coefficient of the concentration measuring a calibration curve created from a known concentration and a reference density color information is measured by reduced concentrations differ from actual is Ru Yes.
The angle between the measuring plate 4B and the optical cell 3 placed apart from each other behind the optical cell 3 is preferably an angle of -5 degrees or more and an angle of 50 degrees or less, and an angle of +10 degrees or more and an angle of +50 degrees or less. It is more preferable, and an angle of +30 degrees or more and an angle of +50 degrees or less is further preferable. If the angle between the measuring plate 4B placed behind the optical cell 3 and the optical cell 3 is small, light from the light source 2 and other outside is reflected on the surface of the optical cell 3 and an error occurs in the measurement result. May occur. In addition, the shadow of the optical cell 3 is reflected in the imaging unit of the measuring plate 4B, which causes an error in the measurement result, and the correlation coefficient between the known density and the calibration curve for density measurement created from the reference density color information is lowered. The measured concentration may differ from the actual one.

In the concentration measuring system 1 of the present invention, the number of optical cells 3 is not particularly limited, but preferably 1 or more and 20 or less can be measured at the same time. By simultaneously measuring the object to be measured stored in the optical cell 3 with the imaging means 8A, the number of measurement steps can be reduced, which is preferable. The fixing jig 7 of the optical cell 3 preferably has a distance of 5 mm or more and 20 mm or less between the bottom surface of the optical cell 3 and the fixing jig 7, and more preferably 10 mm or more and 18 mm or less. It is more preferable that the distance is 12 mm or more and 16 mm or less. At present, it is not known what kind of phenomenon should be separated, but it is presumed that the fixing jig 7 has an optical effect.

In the density measurement system 1 of the present invention, the angle between the center of the optical axis of the light source 2 and the image pickup means 8A with the center of gravity of the reference colored portion and the measurement surface of the measurement portion acquired as the image as the apex is 1 degree or more. It is preferably used when the angle is 135 degrees or less. To explain this positional relationship more easily, in the density measurement system 1 of the present invention, the midpoint of the line connecting the center of gravity of the reference colored portion and the surface giving the color information of the measuring portion and the center of the light source are connected by a straight line. The vertical line is the center line of the optical axis of the light source, and the angle formed by the center line of the optical axis and the straight line connecting the imaging means 8A and the midpoint is 1 degree or more and 135 degrees or less. This angle may be referred to as the angle between the light source and the imaging means. Further, it is more preferable that this angle is 60 degrees or more and 120 degrees or less, and further preferably 80 degrees or more and 100 degrees or less. If this angle is too wide, the white color becomes stronger than it actually is when the reference colored portion 4 is imaged, which causes an error. The correlation coefficient of the calibration curve for density measurement created from the known density and the reference density color information may decrease and the measured density may differ from the actual density.

The materials of the reference plate 4A and the measuring plate 4B may be the same or different. Identical Also, when placing the reference plate 4A at a position away from the imaging means 8A above side than the object 5 includes a reference colored portion provided in the reference plate 4A and a measuring unit provided in the measurement plate 4B It can be suitably used by applying it on the surface of. The materials of the reference plate 4A and the measuring plate 4B are not particularly limited, but a material selected from white paper, drawing paper, a resin material, and a metal material can be preferably used.

The density measuring system 1 of the present invention, the area providing the color information of the measuring unit which is example provided in reference colored portion and the measuring plate 4B provided the reference plate 4A is preferably 1 mm ² or more 500 mm ² or less, the area is 5 mm ² or more 200 mm ² or less is more preferable, the area is 20 mm ² or more 100 mm ² or less still more preferred, area is 20 mm ² or more 25 mm ² or less is most preferred. If this area is too large, it is strongly influenced by the outside and tends to cause an error. Further, if this area is too small, the measurement area tends to decrease, which also tends to cause an error. The shape to be measured can be selected from circle, ellipse, triangle, quadrangle, hexagon, and octagon. As for the quadrangle and the triangle, a rectangle, a square, a rhombus, an isosceles triangle, an equilateral triangle and the like can be arbitrarily selected depending on the shape of the optical cell 3 and the environment in which the image is taken. The area and shape to be imaged can be selected by the image pickup means 8A, or can be preferably set by the image processing means or the calculation means performed after the entire image is captured.

In the concentration measuring system 1 of the present invention, the concentration of the substance to be measured is equal to or lower than the known concentration. If it exceeds the known concentration, after diluting the substance to be measured, the concentration is measured by the concentration measurement system 1 of the present invention, and the concentration before dilution is calculated by multiplying the measured concentration by the dilution ratio. can do. Of course, this dilution rate can also be determined by the calculation means of the concentration measurement system of the present invention.

In the density measurement system 1 of the present invention, any substance can be measured as long as there is a correlation between the density and the color information 4 measured by the imaging means 8A. The substances whose concentration is measured are iron, manganese, magnesium, phosphorus, calcium, potassium, fluorine, hydrogen, chlorine, hexavalent chromium, nickel, aluminum, silver, gold, copper, boron, molybdenum, palladium, zinc and nitrate ion. , Ammonium ion, hydrogen ion, hydroxide ion, indoxyl, ascorbic acid, rotten vegetable, iodine, lycopene , iron, manganese, magnesium, phosphorus, calcium, potassium, hydrogen, chlorine, nickel, aluminum, copper, boron, molybdenum , Palladium, zinc and nitrogen oxides, or complexes formed with ascorbic acid, iodine, methanephetamine and derivatives thereof, cholineesterase and indoxyl acetate or 5-blomo-6-chloro-3-indoxyl butylate or 5-blomo-6. A substance that inhibits the action of cholinesterase and inhibits this color development when it reacts with any one of -chloro-3-indoxyl caprylate and 5-blomo-4-chloro-3-indoxyl palmite and develops color, Escherichia coli, yellow staphylococcus. , Selected from yeast. By measuring the concentration of the brown substance generated by the deterioration of the resin material, it is possible to estimate the deterioration state of the resin material as well. In addition, the amount of polyphenols can be measured for beverages such as wine, alcoholic beverages, and soups.

In the concentration measuring system 1 of the present invention, one substance can be selected and measured from the above-mentioned substances, and for example, as shown in FIGS. 7 and 8, potassium, calcium and humus are used as extracts from soil. , Manganese, magnesium, iron, and / or their oxides, nitrate ions, etc. can be measured at once.

In addition, the concentration of substances can be measured in drinking water containing these substances as ions, plant factories, sewage sludge, extracts from foods, and the like.

Furthermore, since the oxide generation status can be grasped as a surface, the rust generation status in the salt spray test can be easily grasped as a numerical value. Furthermore, when conducting a microbial test, the number of microorganisms present in the liquid is determined from the degree of suspension of the liquid, and the quantity of microorganisms is adjusted. In the concentration measurement system of the present invention, the quantity adjustment of the number of microorganisms is performed. Can also be preferably used.

Hereinafter, the present invention will be described in more detail with reference to Experimental Examples, Examples and Comparative Examples, but the present invention is not limited to the following Examples.

(Example 1)
(apparatus)
A media tablet terminal was used as the image pickup device 8 having the image pickup means 8A. Apple's iPhone 5S (registered trademark) was selected as the media tablet terminal, and image processing means, calculation means , data storage means, display means, and data transmission means were implemented as programs. As the light source, LED line lighting was created and used. Nitrogen (nitrate nitrogen) contained in nitrate ions contained in soil was selected as a measurement target. An optical cell having an optical path length of 8 mm and a fixing jig for positioning were used. A reference plate at a position away from the upper side of the light source than the object to be measured is placed so as to be parallel to the light source, it was placed the measurement plate on the same board. The distance between the measuring plate and the optical cell was 15 mm. The distance between the surface of the optical cell on the imaging means side and the measuring plate was 25 mm. Angle of the optical cell, the shadow of the optical cell is tilted angle -2 ° with respect to the vertical direction on the opposite side of the measuring plate on part of the optical cell so as not to overlap the measuring portion of the measuring plate. The angle between the optical cell and the measuring plate and the reference plate was set to 45 degrees. The angle between the light source and the imaging means was set to 90 degrees. The shape and area of the reference colored portion provided on the reference plate and the measuring portion provided on the measuring ^{plate to give color information were rectangular (area 20 mm 2} ) having a length of 5 mm and a width of 4 mm, and were controlled by the above-mentioned program. The distance between the lower surface of the optical cell and the upper surface of the fixing jig was 15 mm.
(Reference plate )
White paper was colored for color information with a colorant containing 0, 10, 30, 45, 65, 85 and 95% of carbon black as the amount of carbon black, and a reference plate provided with a reference coloring portion was prepared . The RGB values of the color information obtained by the imaging means were divided into 255, and the values were calculated for each amount of carbon black.
(Measuring plate )
A measuring plate was prepared using the same paper as the reference plate.
(Known concentration)
The solution was adjusted so that the concentration of nitrogen (nitrate nitrogen) contained in the nitrate ion was 10, 15, 20, 30 and 50 mg / L. A specified amount of color-developing liquid (for nitrate nitrogen) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, (Equation 1) known density = 58.18-0.245 × reference density color information was obtained. The correlation coefficient at this time was 0.97, which was a good result. At this time, G having the highest correlation was used. In addition, the difference in color for each density could be confirmed visually without any contradiction.
(Measurement of unknown concentration)
In order to verify the effect of the concentration measurement system of the present invention, the solution was adjusted so that the concentration of nitrate nitrogen was 25 mg / L, and this was used as a test sample having an unknown concentration. A specified amount of color-developing liquid (for nitrate nitrogen) manufactured by GK Soil Making Promotion Organization was added to this test sample to develop color. The solution after color development was measured by the concentration measurement system of the present invention, and after following the flowchart shown in FIG. 4, the concentration result was displayed. The measurement results obtained by performing the same measurement three times were 24 mg / L, 25 / L, and 25 mg / L, respectively, which were good results. Similarly, a test sample adjusted to have a concentration of 45 mg / L as nitrate nitrogen was prepared, the color was developed in the same manner, and then the measurement was performed by the concentration measurement system of the present invention and the result was displayed. The measurement results obtained by performing the same measurement three times were 44 mg / L, 45 / L, and 46 mg / L, which were good results, and it was confirmed that the solution concentration of nitrate nitrogen could be measured.

(Example 2)
Phosphoric acid was analyzed using the same equipment, reference plate , and measuring plate as in Example 1.
(Known concentration)
The solution was adjusted to concentrations of 10, 50, 100, 200 and 300 mg / L of phosphoric acid. A specified amount of coloring liquid (for phosphorus) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.94, which was a good result. At this time, R, which had a high correlation, was used. In addition, the difference in color for each density could be confirmed visually without any contradiction.
(Measurement of unknown concentration)
In order to verify the effect of the concentration measurement system of the present invention, the solution was adjusted so that the concentration of phosphoric acid was 250 mg / L, and this was used as a test sample having an unknown concentration. A specified amount of coloring liquid (for phosphorus) manufactured by GK Soil Making Promotion Organization was added to this test sample to develop color. The solution after color development was measured by the concentration measurement system of the present invention, and after following the flowchart shown in FIG. 4, the concentration result was displayed. The measurement results obtained by carrying out the same measurement three times were 245 mg / L and 251 mg / L and 253 mg / L, respectively, which were good results. Similarly, a test sample adjusted to have a concentration of 75 mg / L was prepared, the color was developed in the same manner, and then the measurement was performed by the concentration measurement system of the present invention and the result was displayed. The measurement results obtained by carrying out the same measurement three times were 72 mg / L, 77 mg / L, and 76 mg / L, which were good results. It was confirmed that it can be used for measuring the concentration of ions such as phosphoric acid.

(Example 3)
Magnesium was analyzed using the same equipment, reference plate , and measuring plate as in Example 1.
(Known concentration)
The solution was adjusted to concentrations of 10, 50, 100, 200 and 300 mg / L of magnesium. A specified amount of color-developing liquid (for magnesium) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.96, which was a good result. At this time, R, which had a high correlation, was used. In addition, the difference in color for each density could be confirmed visually without any contradiction.
(Measurement of unknown concentration)
In order to verify the effect of the concentration measurement system of the present invention, the solution was adjusted so that the concentration of magnesium was 200 mg / L, and this was used as a test sample having an unknown concentration. A specified amount of coloring liquid (for magnesium) manufactured by GK Soil Making Promotion Organization was added to this test sample to develop color. The solution after color development was measured by the concentration measurement system of the present invention, and after following the flowchart shown in FIG. 4, the concentration result was displayed. The measurement results obtained by carrying out the same measurement three times were 205 mg / L, 202 mg / L, and 198 mg / L, respectively, which were good results. Similarly, a test sample adjusted to have a concentration of 75 mg / L was prepared, the color was developed in the same manner, and then the measurement was performed by the concentration measurement system of the present invention and the result was displayed. The measurement results obtained by carrying out the same measurement three times were 70 mg / L, 75 mg / L, and 77 mg / L, which were good results. It was confirmed that it can be used to measure the concentration of substances that become cations in aqueous solutions, such as magnesium.

(Example 4)
Calcium was analyzed using the same equipment, reference plate , and measuring plate as in Example 1.
(Known concentration)
The solution was adjusted to concentrations of 100, 200, 300, 400 and 500 mg / L of calcium. A specified amount of coloring liquid (for calcium) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.99, which was a good result. At this time, G having a high correlation was used. In addition, the difference in color for each density could be confirmed visually without any contradiction.
(Measurement of unknown concentration)
In order to verify the effect of the concentration measurement system of the present invention, the solution was adjusted so that the concentration of calcium was 200 mg / L, and this was used as a test sample having an unknown concentration. A specified amount of coloring liquid (for calcium) manufactured by GK Soil Making Promotion Organization was added to this test sample to develop color. The solution after color development was measured by the concentration measurement system of the present invention, and after following the flowchart shown in FIG. 4, the concentration result was displayed. The measurement results obtained by carrying out the same measurement three times were 198 mg / L, 196 mg / L and 202 mg / L, respectively, which were good results. Similarly, a test sample adjusted to have a concentration of 100 mg / L was prepared, the color was developed in the same manner, and then the measurement was performed by the concentration measurement system of the present invention and the result was displayed. The measurement results obtained by carrying out the same measurement three times were 95 mg / L, 95 mg / L, and 98 mg / L, which were good results. It was confirmed that even a substance having a relatively high concentration of about 500 mg / L can be measured.

(Example 5)
Manganese was analyzed using the same equipment, reference plate , and measuring plate as in Example 1.
(Known concentration)
The solution was adjusted to have concentrations of 1, 3, 5, 10 and 25 mg / L of manganese. A specified amount of color-developing liquid (for manganese) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.98, which was a good result. At this time, G having a high correlation was used. In addition, the difference in color for each density could be confirmed visually without any contradiction.
(Measurement of unknown concentration)
In order to verify the effect of the concentration measurement system of the present invention, the solution was adjusted so that the concentration of manganese was 15 mg / L, and this was used as a test sample having an unknown concentration. A specified amount of coloring liquid (for manganese) manufactured by GK Soil Making Promotion Organization was added to this test sample to develop color. The solution after color development was measured by the concentration measurement system of the present invention, and after following the flowchart shown in FIG. 4, the concentration result was displayed. The measurement results obtained by performing the same measurement three times were 14 mg / L, 116 mg / L, and 16 mg / L, respectively, which were good results. Similarly, a test sample adjusted to have a concentration of 2 mg / L was prepared, the color was developed in the same manner, and then the measurement was performed by the concentration measurement system of the present invention and the result was displayed. The measurement result of performing the same measurement three times was 2.4 mg / L, 2.2 mg / L, and 2.3 mg / L, which were good results. It was confirmed that it is possible to measure a solution having a relatively low concentration of about 25 mg / L.

(Example 6)
Using the same equipment, reference plate , and measuring plate as in Example 1, humus (organic matter in soil) was analyzed.
(Known concentration)
As humus, soil humus was extracted with a humus extract manufactured by the Joint Company Soil Development Promotion Organization to prepare a solution containing humus. The solution was adjusted to concentrations 1, 5, 8 and 15% by weight.
(First imaging and preparation of calibration curve for density measurement)
A solution having a known concentration, a reference coloring portion provided on the reference plate, and a measurement portion provided on the measurement plate were imaged by an imaging means to prepare a calibration curve for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.95, which was a good result. At this time, B, which had a high correlation, was used. In addition, the difference in color for each density could be confirmed visually without any contradiction.
(Measurement of unknown concentration)
In order to verify the effect of the concentration measurement system of the present invention, the solution was adjusted so as to have a concentration of 2.5% by weight as humus, and this was used as a test sample having an unknown concentration. After measuring with the concentration measuring system of the present invention and following the flowchart shown in FIG. 4, the concentration result was displayed. The measurement results obtained by carrying out the same measurement three times were 2.6% by weight, 2.7% by weight, and 2.6% by weight, respectively, which were good results. Similarly, a test sample adjusted to have a concentration of 4% by weight was prepared, measured by the concentration measuring system of the present invention, and the results were displayed. The measurement result of performing the same measurement three times was 4.2% by weight, 4.3% by weight, and 4.3% by weight, which were good results. It was confirmed that the concentration of organic matter such as humus can be measured.

(Example 7)
Potassium was analyzed using the same equipment and reference plate as in Example 1. As the measuring plate , a white paper colored with a colorant containing 95% of carbon black was used as the measuring unit.
(Known concentration)
As K ₂ O, it was adjusted solution to a concentration 30,50,100,200 and 266 mg / L. A specified amount of coloring liquid (for potassium) manufactured by GK Soil Making Promotion Organization was added to this solution. In the case of potassium, since it is a suspension, the measuring plate is provided with the black measuring section.
(First imaging and preparation of calibration curve for density measurement)
The solution of known concentration, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit, a provided in the metering plates to prepare a concentration determination calibration curve. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.98, which was a good result. At this time, R, which had a high correlation, was used. In addition, the difference in color for each density could be confirmed visually without any contradiction.
(Measurement of unknown concentration)
In order to verify the effect of the concentration measurement system of the present invention, the solution was adjusted to a concentration of 100 mg / L, and this was used as a test sample having an unknown concentration. A specified amount of coloring liquid (for potassium) manufactured by GK Soil Making Promotion Organization was added to this test sample. This suspension was measured by the concentration measuring system of the present invention, and after following the flowchart shown in FIG. 4, the concentration result was displayed. The measurement results obtained by carrying out the same measurement three times were 105 mg / L, 110 mg / L, and 107 mg / L, respectively, which were good results. Similarly, a test sample adjusted to have a concentration of 200 mg / L was prepared, the color was developed in the same manner, and then the measurement was performed by the concentration measurement system of the present invention and the result was displayed. The measurement results obtained by performing the same measurement three times were 190 mg / L, 207 mg / L, and 206 mg / L, which were good results.

(Example 8)
The concentration measurement system of the present invention was used as a soil analyzer.
The same equipment, reference plate , and measuring plate as in Example 1 were used. 2 g of each of the soil in which eggplants were cultivated for 3 months (Andosols) and the soil in the same field collected before cultivating eggplants were collected, and 18 mL of an extract of pH 4.8 (manufactured by the Joint Company Soil Making Promotion Organization) was collected. The mixture was stirred for an additional 5 minutes to extract nutrients from the soil. After cultivating, the extract obtained from the soil is filtered through a filter paper and then dispensed into an optical cell, which is used as a coloring agent for nitrate nitrogen, phosphorus, magnesium, potassium, manganese, and calcium (all). A specified amount of color-developing liquid manufactured by the Joint Company Soil Making Promotion Organization) was added to develop the color. The humus was extracted from the same soil using the same extract as in Example 7. This was measured by the concentration measuring system of the present invention. A part of the measurement result is shown in FIG. Since it is an extract from soil, the unit of concentration was changed to mg / 100g so that the producer could easily understand it. The measurement was completed with one imaging. The measurement results were nitrate nitrogen 13 mg / 100 g soil, phosphoric acid 16 mg / 100 g soil, potassium 68 mg / 100 g soil, calcium 345 mg / 100 g soil, magnesium 1 mg / 100 g soil, manganese 25 mg / 100 g soil and humus rate 0.9% by weight. Got These results, excluding the concentration of humus, showed a 5% to 20% decrease in the soil before cultivation, suggesting that some of the nutrients were absorbed by the cultivated eggplant. The humus rate was 0.9 wt%, and no change was confirmed. From this result, it was confirmed that the concentration measuring system of the present invention can be used as a soil analyzer.

As described above, Examples 1 to 8 show that the concentration measurement system of the present invention can easily measure the concentration of inorganic substances and organic substances having a correlation between the concentration and the color information that can be expressed by RBG in the visible region. It was. It was also shown that, like potassium, the concentration of a suspension can be effectively measured by using a measuring plate provided with a measuring unit used in the concentration measuring system of the present invention.

(Example 9) Analysis of lycopene In order to analyze lycopene, which is an active ingredient in vegetables, the effect of measuring the concentration of lycopene contained in tomato was verified. A calibration curve for measuring the concentration of lycopene was prepared, and the concentration of lycopene extracted from tomato was measured by the concentration measuring system of the present invention. The existing concentration of lycopene is determined by diluting lycopene purchased as a reagent to a predetermined concentration, determining this by high performance liquid chromatography, measuring the solution prepared for each concentration by the procedure of the concentration measurement system, and creating a calibration curve. did. Ten cultivated small tomatoes (sown April, harvest September) were crushed with a mixer, and lycopene was extracted with a mixed extract of diethyl ether and methanol. The concentration of the extracted lycopene was measured by the concentration measuring system of the present invention and found to be 10 mg / 100 g. The result measured by high performance liquid chromatography was 10.3 mg / 100 g, so it was judged that good results were obtained. It was confirmed that the nutrients contained in vegetables can be measured.

(Example 10) Microorganism test In order to utilize the method for measuring the sterilization rate described in Japanese Patent No. 5252737, the adjustment of microorganisms was carried out by the concentration measurement system of the present invention. As the microorganism, Escherichia coli (Escherichia coli) and Staphylococcus aureus (Staphylococcus aureus) are used, and the absorbance at a wavelength of 632 nm is ^{1.2 × 10 5} / mL and 2.4 × 10 ^{5 / mL.} Solutions were prepared to be 0.3 and 0.6, respectively. The absorbance at a wavelength of 632 nm was measured using an ultraviolet-visible spectrophotometer. The solution at this time was measured by the concentration measuring system of the present invention, and a calibration curve was prepared. ^{On the other hand, the solution was adjusted to adjust 1 × 10 5} cells / mL of Escherichia coli and Staphylococcus aureus, respectively, and the concentration was confirmed by the concentration measurement system of the present invention. The concentration displayed at this time ^{was 1.05 5} pieces / mL. When this solution was applied to a standard agar medium placed on a petri dish and the number of microorganisms was measured, 0.9 to 1.2 × 10 ⁵ cells / mL of Escherichia coli and Staphylococcus aureus were confirmed. This result was a good result as a microbial test. From this result, it was confirmed that the concentration measuring device of the present invention can adjust microorganisms.

(Comparative Example 1)
Nitrate nitrogen was analyzed using the same equipment and reference plate as in Example 1. At this time, the measuring plate was not placed.
(Known concentration)
The solution was adjusted to have concentrations of 10, 15, 20, 30 and 50 mg / L as nitrate nitrogen. A specified amount of color-developing liquid (for nitrate nitrogen) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate (in this comparative example, placing non measurement plate as described above) there was partial measurement plate in Example 1 imaging means A calibration curve for concentration measurement was created. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.30 even at the highest G, and sufficient correlation was not confirmed. It was considered that the cause was that the rear part of the cell was processed as an image due to the absence of the measuring plate.

(Comparative Example 2)
Nitrate nitrogen was analyzed using the same equipment, reference plate , and measuring plate as in Example 1. The distance between the measuring plate and the optical cell was set to 0 mm.
(Known concentration)
The solution was adjusted to have concentrations of 10, 15, 20, 30 and 50 mg / L as nitrate nitrogen. A specified amount of color-developing liquid (for nitrate nitrogen) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.60 even at the highest G, and no good correlation was obtained. Moreover, a result different from that of Example 1 was obtained especially at a high concentration. It was considered that this was because the optical cell and the measuring plate were not separated from each other, so that the RGB values were both lower in the concentrated solution.

(Comparative Example 3)
Nitrate nitrogen was analyzed using the same equipment, reference plate , and measuring plate as in Example 1. The measurement was performed with the angle between the measuring plate and the optical cell being −10 degrees.
(Known concentration)
The solution was adjusted to have concentrations of 10, 15, 20, 30 and 50 mg / L as nitrate nitrogen. A specified amount of color-developing liquid (for nitrate nitrogen) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.65 even at the highest G, and no good correlation was obtained. Results different from those of Example 1 were obtained, especially at low concentrations. It was considered that this was caused by the deviation of the focal point of the measuring plate and the variation of the color information.

(Comparative Example 4)
Nitrate nitrogen was analyzed using the same equipment, reference plate , and measuring plate as in Example 1. The test was conducted with the angle between the imaging means, the light source, and the center of the optical axis of the light source set to 140 degrees.
(Known concentration)
The solution was adjusted to have concentrations of 10, 15, 20, 30 and 50 mg / L as nitrate nitrogen. A specified amount of color-developing liquid (for nitrate nitrogen) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.52 even at the highest G, and the variation became large as a whole. It is considered that this is because the influence of the light from the light source affects the imaging means at the time of imaging, and the variation in color information is the cause.

(Comparative Example 5)
Nitrate nitrogen was analyzed using the same equipment, reference plate , and measuring plate as in Example 1. The distance between the lower surface of the optical cell and the fixing jig was set to 3 mm.
(Known concentration)
The solution was adjusted to have concentrations of 10, 15, 20, 30 and 50 mg / L as nitrate nitrogen. A specified amount of color-developing liquid (for nitrate nitrogen) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, a reference colored portion provided in the reference plate, imaged by the imaging means and the measuring unit provided, to measure plate, a calibration curve was prepared for concentration measurement. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.7 even at the highest G, and the variation became large as a whole. This is because good results were obtained when the distance between the lower surface of the optical cell and the fixing jig was 5 mm or more, and it is considered that the fact that the fixing jig is close to the lower surface of the optical cell is the cause of this variation. It was.

(Comparative Example 6)
Nitrate nitrogen was analyzed using the same equipment and measuring plate as in Example 1. The distance between the lower surface of the optical cell and the fixing jig was set to 3 mm. At this time, the reference plate was not used.
(Known concentration)
The solution was adjusted to have concentrations of 10, 15, 20, 30 and 50 mg / L as nitrate nitrogen. A specified amount of color-developing liquid (for nitrate nitrogen) manufactured by GK Soil Making Promotion Organization was added to this solution to develop color.
(First imaging and preparation of calibration curve for density measurement)
A solution of known concentration that was developed, imaged a measurement unit provided in the measurement plate, the image pickup means, creating the concentration determination calibration curve. From the slope of the calibration curve for density measurement, the relational expression between the known density and the reference density color information was obtained. The correlation coefficient at this time was 0.65 even at the highest G, and the variation became large as a whole. From the comparison with Example 1, it was shown that the use of the reference plate used in the present invention reduced the measurement error.

1 Concentration measurement system 2 Light source 2-1 Light source positioning and holding means 3 Optical cell 4 Reference coloring part 4A Reference plate 4B Measurement plate 5 Measured object 6 Housing 7 Fixing jig 8 Imaging device 8A Imaging means 41 Instruction symbol

By using the concentration measurement system of the present invention, it becomes easy to measure substances of unknown concentration, and the range of use is expanded from agriculture-related to food-related industries.

Claims (4)

An optical cell that can store and hold an object to be measured and that transmits light with an optical path length of 1 mm or more and 10 mm or less and a wavelength of 220 nm or more and 800 nm or less.
A fixing jig for fixing the optical cell and
Light source and
Imaging means and
Said position distant from the imaging means or the object to be measured from the lower side at least two location rather at a position close to the imaging means of different criteria colored portions and the upper side than the device under test accommodated in the optical cell A reference plate with an indicator symbol indicating the position of the reference colored part, and
Measuring with a measuring unit which is acquired as an image by simultaneously said image pickup means and said measured object stored in the optical cell through the are from the object to be measured housed in an optical cell positioned spaced rearwardly the object to be measured Equipped with a board,
At least two different reference colored portions and the measuring portion whose positions are indicated by the instruction symbols by the imaging means are simultaneously acquired as images .
An image processing means for processing the acquired image as color information, a calculation means for calculating the density based on the color information , a data storage display means, and the like.
Concentration measurement system equipped with. The first reference coloring portion of the reference coloring portion provided on the reference plate is subjected to a coloring agent containing 0% or more and less than 50% of carbon black in order to give the first color information as the color information. of a reference colored portion, when 255 dividing the first color information of the first reference colored portion measured as a numerical value by the image pickup means,
R (sometimes written as red) is 100 or more and 255 or less,
G (sometimes referred to as green) is 100 or more and 255 or less,
B (sometimes referred to as blue) is 100 or more and 255 or less.
A second reference color portions of the reference colored portion provided in said reference plate, a carbon black-containing giving and the first color information includes carbon black less than 95% to 50% in order to give the second color information a second reference colored portion subjected to the colorant is a content of 10% or more compared to the amount, number of the second color information of the second reference colored portion is measured by the image pickup means When divided into 255 as
R is 1 or more and 99 or less,
1 or more and 99 or less in G,
B is 1 or more and 99 or less
When three or more color information is used, the amount of carbon black used to give the third color information is the amount used to apply to the first reference coloring portion and the second reference coloring portion, respectively. This is the reference colored portion to which a colorant having a content difference of 10% or more from the amount of carbon black in the above is applied.
The concentration measuring system according to claim 1. Wherein the measuring unit provided in the measurement plate, and said measuring part having been subjected to colorant including carbon black less than 95% 0% in order to provide color information of the measuring portion, which is measured by the image pickup means When the color information of the measuring unit is divided into 255 as numerical values,
R is 1 or more and 255 or less,
1 or more and 255 or less in G,
The concentration measurement system according to any one of claims 1 to 2, wherein B is 1 or more and 255 or less. Claim 1 in which the imaging means is selected from any of a digital camera, a CCD camera, and a media tablet terminal having an image acquisition function capable of simultaneously acquiring at least two different reference coloring units and the measuring unit as images. The concentration measuring system according to any one of 3 to 3.

Images