JP6875998B2 - 生物学的組織試料の分子プロファイルの空間マッピング - Google Patents

生物学的組織試料の分子プロファイルの空間マッピング Download PDF

Info

Publication number
JP6875998B2
JP6875998B2 JP2017554045A JP2017554045A JP6875998B2 JP 6875998 B2 JP6875998 B2 JP 6875998B2 JP 2017554045 A JP2017554045 A JP 2017554045A JP 2017554045 A JP2017554045 A JP 2017554045A JP 6875998 B2 JP6875998 B2 JP 6875998B2
Authority
JP
Japan
Prior art keywords
nucleic acid
oligonucleotide probe
roi
sample
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017554045A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018516541A (ja
JP2018516541A5 (enExample
Inventor
ドリール，マルク ファン
ドリール，マルク ファン
ヴィムベルガー−フリードル，ラインホールト
ピーリク，アンケ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Philips NV
Original Assignee
Koninklijke Philips NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips NV filed Critical Koninklijke Philips NV
Publication of JP2018516541A publication Critical patent/JP2018516541A/ja
Publication of JP2018516541A5 publication Critical patent/JP2018516541A5/ja
Application granted granted Critical
Publication of JP6875998B2 publication Critical patent/JP6875998B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2543/00—Reactions characterised by the reaction site, e.g. cell or chromosome
    • C12Q2543/10—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis
    • C12Q2543/101—Reactions characterised by the reaction site, e.g. cell or chromosome the purpose being "in situ" analysis in situ amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cell Biology (AREA)
JP2017554045A 2015-04-14 2016-04-13 生物学的組織試料の分子プロファイルの空間マッピング Active JP6875998B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15163481 2015-04-14
EP15163481.3 2015-04-14
PCT/EP2016/058067 WO2016166128A1 (en) 2015-04-14 2016-04-13 Spatial mapping of molecular profiles of biological tissue samples

Publications (3)

Publication Number Publication Date
JP2018516541A JP2018516541A (ja) 2018-06-28
JP2018516541A5 JP2018516541A5 (enExample) 2019-10-31
JP6875998B2 true JP6875998B2 (ja) 2021-05-26

Family

ID=52991497

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017554045A Active JP6875998B2 (ja) 2015-04-14 2016-04-13 生物学的組織試料の分子プロファイルの空間マッピング

Country Status (7)

Country Link
US (1) US10724078B2 (enExample)
EP (1) EP3283641B1 (enExample)
JP (1) JP6875998B2 (enExample)
CN (1) CN107709574B (enExample)
BR (1) BR112017021993A2 (enExample)
RU (1) RU2733545C2 (enExample)
WO (1) WO2016166128A1 (enExample)

Families Citing this family (170)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3901280B1 (en) 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US20180057873A1 (en) * 2015-04-17 2018-03-01 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological materials
KR102723865B1 (ko) 2015-12-04 2024-10-29 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
US10724089B2 (en) 2016-10-06 2020-07-28 The Board Of Trustees Of The University Of Illinois Spatial molecular analysis of tissue
US20200370105A1 (en) * 2017-05-23 2020-11-26 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological molecules
KR20250099416A (ko) 2018-03-02 2025-07-01 퀀텀사이트, 인크. 조직으로부터 관심 영역의 단리 및 발현 분석을 위한 방법, 조성물, 및 장치
WO2020047005A1 (en) 2018-08-28 2020-03-05 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN113767175B (zh) 2018-08-28 2025-07-25 10X基因组学股份有限公司 增加空间阵列分辨率
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894585A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating capture probes for spatial analysis
IL293543B2 (en) * 2018-12-31 2024-03-01 Avava Inc Cooling element
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3931354A1 (en) * 2019-02-28 2022-01-05 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
EP3887542A1 (en) 2019-03-22 2021-10-06 10X Genomics, Inc. Three-dimensional spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11514575B2 (en) 2019-10-01 2022-11-29 10X Genomics, Inc. Systems and methods for identifying morphological patterns in tissue samples
CN114902342A (zh) * 2019-10-18 2022-08-12 纳米线科技公司 用于表达谱分析的空间映射的系统和方法
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4589016A3 (en) 2019-11-13 2025-10-08 10x Genomics, Inc. Generating capture probes for spatial analysis
US12154266B2 (en) 2019-11-18 2024-11-26 10X Genomics, Inc. Systems and methods for binary tissue classification
EP4062373B1 (en) 2019-11-21 2025-01-22 10X Genomics, Inc. Spatial analysis of analytes
CN115023734B (zh) 2019-11-22 2023-10-20 10X基因组学有限公司 使用基准对齐对分析物进行空间分析的系统和方法
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
EP4752521A3 (en) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
EP4628596A3 (en) 2020-01-10 2025-12-31 10X Genomics, Inc. METHODS FOR DETERMINING THE LOCATION OF A TARGET NUCLEIC ACID IN A BIOLOGICAL SAMPLE
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
EP4104179B1 (en) 2020-02-13 2026-01-28 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US20230081381A1 (en) 2020-02-20 2023-03-16 10X Genomics, Inc. METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
EP4107285B1 (en) 2020-02-21 2024-10-09 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
CN115803454A (zh) 2020-05-04 2023-03-14 10X基因组学有限公司 空间转录组学转移模式
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021237056A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Rna integrity analysis in a biological sample
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4600376A3 (en) 2020-06-02 2025-10-22 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4164796A4 (en) 2020-06-10 2024-03-06 10x Genomics, Inc. Fluid delivery methods
WO2021252576A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for spatial analysis using blocker oligonucleotides
EP4446430A3 (en) 2020-06-10 2024-12-18 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
JP2023531463A (ja) * 2020-06-18 2023-07-24 ナノストリング テクノロジーズ，インコーポレイティド 酵素的核酸伸長を用いたｉｎ ｓｉｔｕ単一細胞解析のための組成物及び方法
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
EP4189110A1 (en) 2020-07-31 2023-06-07 10X Genomics, Inc. De-crosslinking compounds and methods of use for spatial analysis
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US12580044B1 (en) 2020-09-02 2026-03-17 10X Genomics, Inc. Systems and methods for identifying cells that are antigen-specific for an immunogenic feature
EP4530358A3 (en) 2020-09-15 2025-06-18 10x Genomics, Inc. Methods of releasing an extended capture probe from a substrate and uses of the same
WO2022060953A1 (en) 2020-09-16 2022-03-24 10X Genomics, Inc. Methods of determining the location of an analyte in a biological sample using a plurality of wells
EP4213993A2 (en) 2020-09-18 2023-07-26 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
CN116194590A (zh) 2020-09-18 2023-05-30 10X基因组学有限公司 样品处理设备和图像配准方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022081643A2 (en) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
WO2022087273A1 (en) 2020-10-22 2022-04-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification
WO2022099037A1 (en) 2020-11-06 2022-05-12 10X Genomics, Inc. Compositions and methods for binding an analyte to a capture probe
AU2021385065A1 (en) 2020-11-18 2023-06-15 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022147296A1 (en) 2020-12-30 2022-07-07 10X Genomics, Inc. Cleavage of capture probes for spatial analysis
US20240068017A1 (en) 2020-12-30 2024-02-29 10X Genomics, Inc. Methods for analyte capture determination
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
US20240093290A1 (en) 2021-01-29 2024-03-21 10X Genomics, Inc. Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
WO2022197490A1 (en) * 2021-03-16 2022-09-22 Scale Biosciences Inc. Method for adding spatially-addressable barcodes to nucleic acids of a cellular sample in situ
EP4524261B1 (en) 2021-03-18 2026-07-22 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
AU2022256031A1 (en) 2021-04-05 2023-10-12 10X Genomics, Inc. Recombinant ligase composition and uses thereof
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022226057A1 (en) 2021-04-20 2022-10-27 10X Genomics, Inc. Methods for assessing sample quality prior to spatial analysis using templated ligation
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022271820A1 (en) 2021-06-22 2022-12-29 10X Genomics, Inc. Spatial detection of sars-cov-2 using templated ligation
US20240263218A1 (en) 2021-07-13 2024-08-08 10X Genomics, Inc. Methods for spatial analysis using targeted probe silencing
EP4779305A2 (en) 2021-07-13 2026-07-22 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
WO2023018799A1 (en) 2021-08-12 2023-02-16 10X Genomics, Inc. Methods, compositions and systems for identifying antigen-binding molecules
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US20240378734A1 (en) 2021-09-17 2024-11-14 10X Genomics, Inc. Systems and methods for image registration or alignment
WO2023076345A1 (en) 2021-10-26 2023-05-04 10X Genomics, Inc. Methods for spatial analysis using targeted rna capture
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4430398B1 (en) 2021-11-10 2025-12-31 10X Genomics, Inc. METHODS FOR IDENTIFYING ANTIGEN-BINDING MOLECULES
US20230167495A1 (en) 2021-11-30 2023-06-01 10X Genomics, Inc. Systems and methods for identifying regions of aneuploidy in a tissue
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023150163A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from lymphatic tissue
WO2023150171A1 (en) 2022-02-01 2023-08-10 10X Genomics, Inc. Methods, compositions, and systems for capturing analytes from glioblastoma samples
US20250163501A1 (en) 2022-02-01 2025-05-22 10X Genomics, Inc. Methods, kits, compositions, and systems for spatial analysis
EP4479551A1 (en) * 2022-02-14 2024-12-25 Miltenyi Biotec B.V. & Co. KG Direct synthesis of oligonucleotides on microtomed tissue slices
EP4479933A1 (en) 2022-02-15 2024-12-25 10x Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
WO2023172670A2 (en) 2022-03-11 2023-09-14 10X Genomics, Inc. Sample handling apparatus and fluid delivery methods
US12637706B2 (en) 2022-03-23 2026-05-26 10X Genomics, Inc. Methods and compositions related to microsphere surface gelation
US20250251403A1 (en) 2022-04-12 2025-08-07 10X Genomics, Inc. Compositions and methods for generating and characterizing recombinant antigen binding molecules
WO2023215552A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Molecular barcode readers for analyte detection
US20240002902A1 (en) 2022-05-06 2024-01-04 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
US12559791B2 (en) 2022-05-06 2026-02-24 10X Genomics, Inc. Methods and compositions for in situ analysis of V(D)J sequences
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
WO2023229988A1 (en) 2022-05-23 2023-11-30 10X Genomics, Inc. Tissue sample mold
US20250312793A1 (en) 2022-05-24 2025-10-09 10X Genomics, Inc. Porous structure confinement for convection suppression
WO2023250077A1 (en) 2022-06-22 2023-12-28 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4554973A1 (en) 2022-07-13 2025-05-21 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples
WO2024015578A1 (en) 2022-07-15 2024-01-18 10X Genomics, Inc. Methods for determining a location of a target nucleic acid in a biological sample
EP4565870A1 (en) 2022-08-05 2025-06-11 10x Genomics, Inc. Systems and methods for immunofluorescence quantification
WO2024036191A1 (en) 2022-08-10 2024-02-15 10X Genomics, Inc. Systems and methods for colocalization
WO2024035844A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Methods for reducing capture of analytes
EP4577670A1 (en) 2022-08-24 2025-07-02 10X Genomics, Inc. Compositions and methods for antigenic epitope mapping in biological samples
WO2024081212A1 (en) 2022-10-10 2024-04-18 10X Genomics, Inc. In vitro transcription of spatially captured nucleic acids
US20240191297A1 (en) 2022-10-14 2024-06-13 10X Genomics, Inc. Methods, compositions, and systems for assessing biological sample quality
WO2024086167A2 (en) 2022-10-17 2024-04-25 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4685239A1 (en) 2022-11-09 2026-01-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
WO2024116670A1 (ja) * 2022-11-30 2024-06-06 コニカミノルタ株式会社 マイクロアレイデバイス、マイクロアレイデバイスの製造方法、検査キット、検査システム、及び検査方法
WO2024137826A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Analysis of analytes and spatial gene expression
WO2024145224A1 (en) 2022-12-29 2024-07-04 10X Genomics, Inc. Compositions, methods, and systems for high resolution spatial analysis
WO2024145441A1 (en) 2022-12-29 2024-07-04 10X Genomics, Inc. Methods, compositions, and kits for determining a location of a target nucleic acid in a fixed biological sample
WO2024145445A1 (en) 2022-12-30 2024-07-04 10X Genomics, Inc. Methods of capturing target analytes
WO2024145491A1 (en) 2022-12-30 2024-07-04 10X Genomics, Inc. Methods, compositions, and kits for multiple barcoding and/or high-density spatial barcoding
WO2024206603A1 (en) 2023-03-28 2024-10-03 10X Genomics, Inc. Methods, compositions, and kits for reducing analyte mislocalization
WO2024220882A1 (en) 2023-04-21 2024-10-24 10X Genomics, Inc. Methods and compositions for detecting nucleic acids in a fixed biological sample
WO2024238625A1 (en) 2023-05-15 2024-11-21 10X Genomics, Inc. Spatial antibody data normalization
WO2024238900A1 (en) 2023-05-17 2024-11-21 10X Genomics, Inc. Methods of reducing mislocalization of an analyte
WO2024238992A1 (en) 2023-05-18 2024-11-21 10X Genomics, Inc. Engineered non-strand displacing family b polymerases for reverse transcription and gap-fill applications
WO2024254316A1 (en) 2023-06-07 2024-12-12 10X Genomics, Inc. Compositions and methods for identifying blood clots in biological samples
WO2024258911A1 (en) 2023-06-13 2024-12-19 10X Genomics, Inc. Sequence specific dna binding proteins
WO2025029605A1 (en) 2023-07-28 2025-02-06 10X Genomics, Inc. Methods and compositions for detecting genetic variants using capture probes and primer extension
WO2025029627A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods, compositions, and kits for spatial detection of genetic variants
WO2025043076A1 (en) 2023-08-24 2025-02-27 10X Genomics, Inc. Methods, kits, and compositions for spatial detection of genetic variants
WO2025072119A1 (en) 2023-09-26 2025-04-03 10X Genomics, Inc. Methods, compositions, and kits for detecting spatial chromosomal interactions
WO2025090912A1 (en) 2023-10-26 2025-05-01 10X Genomics, Inc. Spatial arrays containing reusable master probes
WO2025096581A1 (en) 2023-10-31 2025-05-08 10X Genomics, Inc. Methods of spatial analysis and single nuclei sequencing
WO2025101377A1 (en) 2023-11-09 2025-05-15 10X Genomics, Inc. Matrix-assisted spatial analysis of biological samples
WO2025101864A1 (en) 2023-11-09 2025-05-15 10X Genomics, Inc. Methods, compositions, and kits for reducing mislocalization of analytes in spatial analysis assays
US12442729B2 (en) 2023-11-13 2025-10-14 Singular Genomics Systems, Inc. Devices useful for sample manipulation
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
WO2026024934A1 (en) 2024-07-25 2026-01-29 10X Genomics, Inc. Methods, compositions, and kits for spatial analysis by tagmentation
WO2026043885A1 (en) 2024-08-20 2026-02-26 10X Genomics, Inc. Methods and systems for preparing a nucleic acid library
WO2026135910A1 (en) 2024-12-17 2026-06-25 10X Genomics, Inc. Spatial analysis of whole genome and target genomic dna
WO2026136945A1 (en) 2024-12-20 2026-06-25 10X Genomics, Inc. Methods and compositions for selective control of signal generation efficiency

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1680757A4 (en) 2003-10-08 2006-11-22 Lifespan Biosciences Inc AUTOMATED MAPPING OF TISSUE SAMPLES ON MICROSCOPE BLADE AND ACQUISITION OF IMAGES
RU2553078C2 (ru) * 2009-04-28 2015-06-10 Конинклейке Филипс Электроникс Н.В. Способ микродиссекции и система обработки информации
KR101866401B1 (ko) * 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
US20150262329A1 (en) 2012-10-03 2015-09-17 KONINKLIJKE PHILIPS N.V. a corporation Combined sample examinations
CN111500680B (zh) * 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统

Also Published As

Publication number Publication date
RU2017135434A (ru) 2019-04-08
EP3283641B1 (en) 2019-11-27
BR112017021993A2 (pt) 2018-07-31
JP2018516541A (ja) 2018-06-28
WO2016166128A1 (en) 2016-10-20
CN107709574A (zh) 2018-02-16
RU2017135434A3 (enExample) 2019-10-02
CN107709574B (zh) 2021-10-01
EP3283641A1 (en) 2018-02-21
US10724078B2 (en) 2020-07-28
RU2733545C2 (ru) 2020-10-05
US20180112261A1 (en) 2018-04-26

Similar Documents

Publication Publication Date Title
RU2733545C2 (ru) Пространственное картирование молекулярных профилей образцов биологических тканей
JP5916166B2 (ja) 組織試料中の核酸の局在化された検出、又は空間的検出のための方法及び生成物
EP4151748B1 (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
JP5951755B2 (ja) 定量的ヌクレアーゼプロテクションアッセイ（ｑＮＰＡ）法および定量的ヌクレアーゼプロテクション配列決定（ｑＮＰＳ）法の改善
CN116848263A (zh) 用于分析物检测的方法和组合物
Hodges et al. Hybrid selection of discrete genomic intervals on custom-designed microarrays for massively parallel sequencing
CN108350484B (zh) Rna分析的组合物和方法
EP3177740B1 (en) Digital measurements from targeted sequencing
JP6785839B2 (ja) Ｄｎａ試料を分析するためのプローブ組及びその使用方法
CN116406428A (zh) 用于使用酶促核酸延伸进行原位单细胞分析的组合物和方法
US11555185B2 (en) Target enrichment
AU2019280712A1 (en) Methods of producing nucleic acid libraries and compositions and kits for practicing same
US10927405B2 (en) Molecular tag attachment and transfer
US20200140943A1 (en) A method for the clustering of dna sequences
JP6983906B2 (ja) ライブラリーの定量および定性
Dubey et al. Microarray technology: basic concept, protocols, and applications
HK40129148A (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40081866B (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40062809B (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
HK40062808B (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190411

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190920

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20190920

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20191003

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191015

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200107

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200415

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200526

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200813

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201125

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210119

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210301

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20210330

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20210423

R150 Certificate of patent or registration of utility model

Ref document number: 6875998

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE

Ref document number: 6875998

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250