JP6858324B1 - A device for confirming the mosquito repellent function and a test method using it - Google Patents

Abstract

PROBLEM TO BE SOLVED: To perform a highly accurate test on a specific organism because the size and shape of the device are not specified in addition to the existing mosquito repellent confirmation test device for a wide range of insects. .. SOLUTION: A feeding container having an opening, a container having an openable and closable mosquito inlet, an artificial skin for storing the attracting bait and covering the surface of the attracting bait, and a warmer for keeping the feeding container warm. The container is provided with an attracting sample having an attractive sample, and the container has an opening having a diameter of 70 mm or more and 100 mm or less, a length of 130 mm or more and 260 mm or less, and a volume of 500 mL or more and 1 L or less. A mosquito-proof test device that is a cylindrical container having a portion and in which an attracting sample is attached to the opening. [Selection diagram] None

Description

The present invention relates to a device for confirming an insect repellent function.
More specifically, the present invention relates to a device capable of determining the mosquito-proof function of a substance processed with a mosquito-proof component.

In the life of human society, there are insects that have various effects on living things, such as unpleasant pests and sanitary pests. Among them, mosquitoes may transmit infectious diseases, and the insect repellent function against mosquitoes is regarded as important, and various products using insect repellent components have been developed.
However, test results from an objective point of view are required to show that such products have an insect repellent function. Therefore, there is a demand for a device for objectively determining the insect repellent function as in the present invention.

For example, regarding the test method of Patent Document 1, a mosquito that has not sucked blood for 1 to 2 weeks after emergence is put in a cloth cage, and a bag-shaped test cloth is put on one arm of the experimenter and inserted, and the mosquito stops for a specified time. It is stated that the number and the number of mosquitoes showing blood-sucking behavior are counted to obtain the repellent index.
In Patent Document 2, mosquitoes that have not been sucked for 1 to 2 weeks after emergence are placed in a cloth cage, and rats treated with the test product and the control product are placed in wire mesh bags and fixed, and the number of stops after 3 minutes is determined. It is described that the number of mosquitoes showing blood-sucking behavior is counted and the repellent index is calculated from the numbers of the test product and the control group.
The test apparatus of Patent Document 3 is provided with a pair of rails on the outer surface of the housing, and the sample holder is slid on the rails to expose the sample to the inside of the housing.

Japanese Unexamined Patent Publication No. 2005-0234339 Japanese Unexamined Patent Publication No. 2006-169209 Patent No. 6200126

Since the human body, rat, and the like are used for the tests of Patent Documents 1 and 2, it is difficult to obtain an objective evaluation because the state of the living body on that day affects the test.
Since the test apparatus of Patent Document 3 is provided with a pair of rails on the outer surface of the housing, the shape of the housing is at least as large as the rails can be formed. Then, at least the rail forming surface has a shape having a sufficient area for forming a square shape or a pair of rails. As a result, the exposed surface of the sample on the inside of the containment surface becomes only a part of the surface on which the exposed surface is formed. Then, more mosquitoes are needed to increase the density of mosquitoes inside the containment.
In the present invention, as a device for objectively determining the insect repellent function, a rail is not provided, and the area of the exposed surface of the sample occupying the inside surface of the container is increased, so that the device is intended for a more accurate mosquito repellent test. The subject is the provision of equipment.

The mosquito repellent test device of the present invention is closed except for the opening 11 and the mosquito inlet 2, and by providing the opening 11 with a cushioning material, the attracting sample can be attached without a gap, whereby the mosquito inlet 2 can be attached. Containment body 1 whose size and shape are specified as described below so that mosquitoes cannot enter and exit from other than the above, and it is easy to attract mosquitoes to attracted bait.
A mosquito inlet 2 that opens and closes freely to prevent mosquitoes from entering and exiting during the test, and a feeding container 7 for holding a liquid attractant bait 12 that is exposed to the inside of the housing 1. An attractant sample 14 having a diameter of 60 mm, which is composed of an artificial skin 5 serving as a lid, a heat insulator 6 for adjusting the temperature of the feeding container 7 including the attractant bait 12 and the artificial skin 5, and a test sample 4 in contact with the artificial skin 5. To be equipped with.

In the present invention, as a device for objectively determining the insect repellent function, a rail is not provided, and the area of the exposed surface of the sample occupying the inside surface of the container is increased, so that the mosquito repellent test is more accurate. Provide the device.

Front view of the mosquito repellent test apparatus of the present invention Side view of the mosquito repellent test apparatus of the present invention Detailed view of the test sample portion of the mosquito repellent test apparatus of the present invention Installation diagram when conducting a mosquito repellent function confirmation test using the mosquito repellent test device of the present invention

The feature of the test device is that a circular sample is connected to the end face of the cylindrical container, and each mosquito in the container basically moves in the length direction of the cylindrical container to reach the sample surface, depending on the location inside the container. The distance to the sample can be made uniform. Therefore, the accuracy of the test is improved, and in the case of a test result that attracts more mosquitoes, more mosquitoes are attracted, and in the case of a test result that cannot attract more mosquitoes, less mosquitoes are attracted.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. In this disclosure, the mosquito refers to an insect belonging to the family Nematocera, Flies.

FIG. 1 is a front view of the mosquito repellent test device of the present invention, and FIG. 2 is a side view of the mosquito repellent test device of the present invention. In the mosquito repellent test device, a mosquito inlet 2 is formed, and an attraction sample 14 composed of a test sample 4, an artificial skin 5, a heat insulator 6, a feeding container 7, and an attracting bait 12 to be put into the opening 11 is formed. By wearing the mosquito, all but the mosquito inlet 2 are closed so that mosquitoes cannot pass through, and the mosquito is composed of a housing 1 capable of accommodating the mosquito inside.
The opening 11 can be fitted with the attracted sample 14 without a gap so that mosquitoes cannot enter and exit by using a cushion material, a plastic material, or the like.
The container 1 is made of a cylindrical container having a diameter of 70 mm or more and 100 mm or less, a length of 130 mm or more and 260 mm or less, and a volume of 500 mL or more and 1 L or less. The housing 1 includes a plate-shaped mosquito inlet cover 3 that can cover the mosquito inlet 2 from the outside.

At least a part of the housing 1 is made of a transparent or translucent material, and the inside can be confirmed. The housing body 1 is provided with a mosquito inlet 2 and an air supply port 9. The air supply tube 8 that sends the excitatory substance to the housing 1 is inserted into the air supply port 9. An exhaust port 10 is provided on the side surface of the housing 1 in order to prevent excessive filling of the excitatory substance.

The mosquito inlet cover 3 can be completely shielded from the outside during the test by closing the mosquito inlet 2. The shape of the mosquito inlet cover 3 may be any shape. If it is a slide type, it shall be made of a transparent or translucent material and shall not interfere with the internal confirmation. When using a plug-shaped mosquito input cover, use a rubber or plastic plug to block it without any gaps.

FIG. 3 shows the details of the attracted sample 14. The attracted sample 14 is composed of a test sample 4, an artificial skin 5, a warmer 6, a feeding container 7, and an attracted bait 12 to be put into the feeding container 7.

The test sample 4 is mainly a woven fabric that has been subjected to insect repellent treatment or a woven fabric that has not been subjected to insect repellent treatment. The sample is not limited to the woven fabric, and may be a non-woven fabric, a film, paper or a resin plate as long as it is a substance that does not completely block the attractant bait 12 and does not hinder the mosquitoes from sucking blood.

The artificial skin 5 is an intestinal casing, a plastic paraffin film, an artificial casing, or the like collected from pigs or sheep in order to hold artificial blood that is a liquid. In order to keep the blood-sucking rate from mosquitoes constant, it is better to wash away the salt from the intestinal casing collected from pigs and sheep and soak it in water overnight.

The warmer 6 includes a temperature sensor and a heater (not shown) that keep the liquid of the attracting bait 12 at a predetermined temperature. Then, the temperature heater and the sensor are connected to a control device (not shown). The warmer 6 includes a feeding container 7 having an open upper portion, and can inject the attracted bait 12. The attractant bait 12 mainly uses a liquid containing horse or cow blood as a main component. The temperature of the attractant bait 12 is controlled to be maintained at 34 ± 2 degrees, which is about the body temperature of the animal.

The attracted sample 14 is sealed by covering the upper part of the feeding container 7 provided with the warmer 6 with artificial skin 5, and the attracting bait 12 is injected into the feeding container 7 using a syringe or the like so that air does not enter to keep the heat. It is created by fixing the vessel 6 and the test sample 4 so as to be in close contact with the feeding container 7.

An example of the test procedure is shown. First, a mosquito repellent test device is prepared. The feeding container 7 is created according to the procedure described in the previous section, and is combined with the container 1. An air supply tube 8 for supplying an excitatory substance to the mosquito repellent test device is attached from the air supply port 9. A mosquito is thrown in from the mosquito slot 2 and an excitatory substance is appropriately supplied. The mosquito control performance is evaluated by measuring the number of mosquitoes stationary on the test sample.

In the mosquito repellent test device, the feeding container 7 and the housing body 1 are not operated by rails, but a cushion material and a plastic plate are made in the size of a warmer 6 in which the test sample 4 is mounted in the opening 11 of the housing body 1. The position is maintained by adjusting with and mounting without gaps, or by mounting with screws without gaps. Confirm that the mosquito inlet 2 is closed. As a result, mosquitoes cannot freely enter and exit from the start to the end of the test, and the test in a closed space becomes possible. Further, by eliminating the right-angled portion / flat surface portion of this device as much as possible, it is possible to prevent mosquitoes from gathering at the right-angled portion / flat surface portion.

FIG. 4 shows an installation diagram when the test is actually carried out. When conducting the test, the mosquito control test device is tilted at 45 ± 20 degrees and fixed to perform the test.

When mosquitoes are thrown in from the mosquito slot 2, the mosquitoes are collected in a tube or the like and blown at once. By such a method, the deviation of the start time can be minimized. The number of mosquitoes to be introduced will be adjusted according to the test situation.

Carbon dioxide gas is used as the excitatory substance supplied from the air supply tube 8. If the supply amount is too large, the mosquitoes will fall asleep, so adjust the input amount.

Regarding the evaluation method of mosquito resistance, in addition to measuring the number of mosquitoes stationary in the test sample 4, the number of mosquitoes that have been knocked down in the test sample provided with the mosquito prevention function is measured, and the mosquitoes after a certain period of time have passed. Various methods such as checking the number of blood sucked by crushing the abdomen of the mosquito can be considered.

Table 1 shows the difference in mosquito attraction ability between the mosquito repellent confirmation test according to the present invention and the “JIS L1950-1 fabric mosquito repellent test method”. The maximum mosquito rest rate was calculated by the number of mosquitoes still / the total number of mosquitoes input.
The container of "JIS L1950-1 fabric mosquito repellent test method" is a rectangular parallelepiped with a volume of 15.2 L and a volume of 200 mm x 200 mm x 380 mm, and the sample surface has a diameter of 60 mm arranged in three places on one surface of the container. However, while the number of mosquitoes input is 30, the housing in the present invention is a cylinder with a diameter of 70 mm, a length of 130 mm, and a volume of 500 mL, a sample surface of 60 mm in diameter at one location, and 20 mosquitoes. did.
In the present invention, the linear distance from each mosquito inside the container to the sample is short, and the attraction performance to mosquitoes is improved, so that the difference in repellent performance depending on the test sample is likely to occur, and the accuracy of the test is improved.

蚊の頭数を５頭、１０頭、１５頭に減らした場合においても、同等に約６割の最多蚊静率が得られる。

Even when the number of mosquitoes is reduced to 5, 10, or 15, the maximum mosquito quiescence rate of about 60% can be obtained.

As the basis of the present invention in Table 2, the results of using the container 1 prepared with a diameter of 70 mm, the length of 130 mm, and the volume of 500 mL and the container 1 prepared with a diameter of 70 mm, a length of 260 mm, and a volume of 1 L are used. Describe the results. The maximum mosquito rest rate was calculated by the same method as described above.
The ratio of the area of the attracted sample 14 to the total area inside the container is large, the density of mosquitoes is high with respect to the volume of the container, and the linear distance to the attracted sample 14 from any position in the container is short.

1. 1. Containment body 2. Mosquito slot 3. Mosquito slot cover 4. Test sample 5. Artificial skin 6. Incubator 7. Feeding container 8. Air supply tube 9. Air supply port 10. Exhaust port 11. Opening 12. Attractive bait 13. Liquid inlet and plastic stopper 14. Attracted sample

Claims (4)

An opening and a containment with a mosquito slot that can be opened and closed,
A feeding container with artificial skin that stores the attracted bait and covers the surface of the attracted bait,
An attracting sample having a warmer for keeping the feeding container warm,
With
Having the attracted sample mounted tightly in the opening
The container is a cylindrical container having an opening having a diameter of 70 mm or more and 100 mm or less, a length of 130 mm or more and 260 mm or less, and a volume of 500 mL or more and 1 L or less. apparatus. A sealing member is provided at the opening of the container, and the attractant sample is mounted without a gap so that mosquitoes cannot enter and exit. At least a part of the container is made of a transparent or translucent material, and the mosquito inlet is accommodated. The mosquito repellent test according to claim 1 or 2, which is provided in the central part of the body, has an exhaust port for preventing the filling of the excitatory substance, and has a supply port for supplying the excitatory substance in the accommodating body. apparatus. For the attracted sample, any one of claims 1 to 3 in which the surface of the feeding container in which the liquid attracted food is stored is covered with artificial skin, the test sample is brought into close contact with the artificial skin surface, and the feeding container is attached to a warmer. The mosquito-proof test device described in Crab. A step of installing the mosquito repellent test device according to any one of claims 1 to 3 at an angle of 45 ± 20 degrees, a step of throwing mosquitoes into the containment body at once, and a step of appropriately injecting an excitatory substance. A mosquito repellent test method including a measurement step for measuring the effect of the sample.

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