JP6842079B2 - Method for producing reduced minerals and method for producing reduced minerals having intracellular sugar uptake activity - Google Patents

Description

The present invention relates to a method for producing a reduced mineral effective for a living body by adding a reducing power derived from hydrogen to a mineral component essential for the living body.

Minerals are the basic substances that are indispensable for building the body and keeping its function normal. Calcium and iron are generally well known as minerals, but various minerals such as zinc, fluorine, sodium, potassium, and magnesium work together with vitamins, hormones, and enzymes to build the body and maintain its function. Currently, the minerals that have been confirmed as necessary nutrients for us are calcium, phosphorus, magnesium, sulfur, sodium, potassium, chlorine, which are seven major minerals, and iron, zinc, copper, manganese, and iodine, which are nine trace minerals. , Selene, molybdenum, chromium, and fluorine, a total of 16 types, but the need for other minerals has also been reported, and it has been pointed out that deficiency of these minerals causes various disorders.

In addition, excess active oxygen and free radicals that are produced in the body or are externally derived and exist in the body cause non-specific chemical reactions to various substances in the body and can damage cells. , Its harmfulness has been pointed out.
Since antioxidants inactivate active oxygen and radicals, they are expected to eliminate excess radicals generated in the living body by their ingestion.

Due to the above circumstances, various substances containing abundant minerals and having an antioxidant effect have been developed in recent years.
Patent Document 1 describes a gene oxidative damage suppressing action consisting of a heat-treated salt obtained by reducing and firing a salt containing sodium chloride (NaCl) as a main component with a carbon source at a temperature of 500 ° C. to 1100 ° C. for 0.5 to 4 hours. It describes a salt.

Further, Patent Document 2 describes an alkaline health food having a large reducing power and containing a large amount of an alkaline component obtained by firing a natural salt mixed with a plant extract having a reducing power at 700 ° C. to 1600 ° C. in three steps. The manufacturing method is described.

In Patent Document 3, water is added to a raw material containing coral calcium powder and wheat flour and kneaded, and the mixture is fired in two stages of oxidation firing and reduction firing to obtain a reduced calcined product and obtained as a fine powder. A method for producing a negative hydrogen ion is described.

Japanese Unexamined Patent Publication No. 2003-313132 WO2003 / 024244 Japanese Patent No. 4404657

However, a substance having a mineral component and also having an antioxidant effect, which is prepared by a conventional method, has a problem in maintaining its reducing power and reducing power, or in a preparation method that requires equipment and time.

The present invention has been made in view of such circumstances, and contains abundant minerals that have been pointed out to cause various disorders in the living body if insufficient, and further, with respect to active oxygen, which is a substance harmful to the body. It is an object of the present invention to provide a method for producing a reduced mineral having high reducing power and stable activity. Here, the reduced mineral means a mineral having a reducing power.

For the above purpose, the present inventors thought that it might be possible to produce a substance useful for the living body by reducing minerals essential for the living body to have antioxidant properties, and as a result of diligent research, the living body We have established a method for producing reduced minerals that can show useful effects on living organisms by adding hydrogen-derived reducing power to essential mineral components.
The reduced mineral produced by the method of the present invention maintains active oxygen scavenging activity for an extremely long period of time, which is not found in ordinary antioxidants, and has strong intracellular active oxygen scavenging ability, that is, antioxidant ability. doing.

The method for producing a reduced mineral according to the first invention according to the above object is incineration ash derived from one or both of plants and animals containing a plurality of minerals in a gas furnace filled with nitrogen gas, or the incineration ash. The first step of adding a powdery inorganic substance with the same composition as
After the first step, the gas furnace is degassed, and the gas furnace is filled with hydrogen gas in the range of 1 to 1000 mM for 1 g of the incineration ash or the powdery inorganic substance.
The third step of heating the gas furnace to produce reduced minerals so that the temperature inside the furnace is in the range of 600 to 1000 ° C.
After the third step, there is a fourth step of recovering the reduced mineral by lowering the temperature in the furnace to room temperature and performing exhaust gas and nitrogen substitution.
The method for producing a reduced mineral according to the second invention according to the above object is to use incinerated ash derived from one or both of plants and animals containing a plurality of minerals or a powdery inorganic substance having the same composition as the incinerated ash. The first step of filling the filled gas furnace with nitrogen gas,
After the first step, the gas furnace is degassed, and the gas furnace is filled with hydrogen gas in the range of 1 to 1000 mM for 1 g of the incineration ash or the powdery inorganic substance.
The third step of heating the gas furnace to produce reduced minerals so that the temperature inside the furnace is in the range of 600 to 1000 ° C.
After the third step, there is a fourth step of recovering the reduced mineral by lowering the temperature in the furnace to room temperature and performing exhaust gas and nitrogen substitution.
Here, the equivalent powdery inorganic ash and components, Ru powdery inorganic der to equalize the ash and component 85% or more.

Here, the material of the incineration ash of plants and / or animals includes any of those derived from animals and plants, and includes wastes such as food wastes and waste materials, whether they are animals or plants themselves or processed products. In addition, it is desirable to remove the organic matter by performing an ashing treatment in advance if the organic matter remains.

In the method for producing reduced minerals according to the first and second inventions, it is preferable that the first step and the second step are carried out at normal temperature and pressure.

In the method for producing reduced minerals according to the first and second inventions, in the second step, hydrogen is produced so that the hydrogen gas is 1.4 to 100% by volume at normal temperature and pressure with respect to the furnace capacity. It is preferable to fill only the gas or hydrogen gas and nitrogen gas.
Here, the normal temperature and the normal pressure mean the temperature and the pressure before starting the heating in the third step, the normal temperature means, for example, between -1 degree and 40 degrees, and the normal pressure means the normal pressure. It means the pressure when neither decompression nor pressurization is performed, and usually means equal to atmospheric pressure.
Further, the hydrogen gas and the nitrogen gas to be filled in the gas furnace may be mixed in advance and inserted into the furnace, or they may be separately inserted into the furnace. Further, it is also possible to once lower the temperature in the furnace and repeatedly fill the furnace with hydrogen gas and nitrogen gas.

In the method for producing reduced minerals according to the first and second inventions, the temperature inside the furnace in the third step is preferably 700 to 900 ° C.
In the method for producing reduced minerals according to the first and second inventions, it is preferable to maintain the temperature in the furnace for 0.5 to 6 hours in the third step.
Here, in order to maintain the temperature in the furnace for 0.5 to 6 hours, it is also included to repeat the process of lowering the temperature once, raising the temperature again, and maintaining the temperature for 0.5 to 6 hours.

The method for producing a reduced mineral having an intracellular sugar uptake activity according to the third invention according to the above object is derived from one or both of a plant and an animal containing a plurality of minerals in a gas furnace filled with nitrogen gas. after degassing put ash, hydrogen gas to ash 1g packed in the range of 1 to 1000 mm, the furnace temperature is heating the gas furnace to be in the range of 600 to 1000 ° C. reduction A type mineral is prepared, lowered to room temperature, exhausted and replaced with nitrogen to recover the reduced type mineral.

The method for producing reduced minerals according to the first and second inventions is essential for living organisms because it adds hydrogen-derived reducing power to mineral-rich incinerated ash or powdery inorganic substances having the same composition as incinerated ash. It is possible to produce a substance containing a mineral component and at the same time having an antioxidant action against active oxygen harmful to the living body.
Further, according to the method of the present invention, the activity is sufficiently maintained for one month even when stored under normal conditions not under anaerobic conditions, and in the case of refrigerated storage, the activity remains even after half a year and is stable even in an aqueous solution. It has become possible to produce a reduced mineral having an extremely excellent property of retaining the reduced reducing activity.
In particular, the first method of reduced mineral according to the second invention, because it uses the ash of plant and / or animal, can easily raise the material, also help waste treatment.
Furthermore, the method for producing a reduced mineral having intracellular sugar uptake activity according to the third invention is effective in treating diabetes, which promotes cellular sugar uptake and can be expected to reduce insulin resistance occurring in diabetic patients. It has become possible to provide a variety of reduced minerals.

It is a graph which shows the DPPH radical scavenging activity of the reduced mineral in Example 1 produced by the manufacturing method of the reduced mineral which concerns on this invention. It is a graph which shows the change of the activity of the reduced minerals prepared by changing the making temperature in Example 2. FIG. It is a graph which shows the result of having measured the change of the activity of the reduced mineral prepared by changing the making temperature in Example 3 by the ORAC measuring method. It is a graph which shows the change of the reducing power of the reduced minerals produced by changing the hydrogen concentration in a furnace in Example 4. It is a graph which shows the change of the reducing power of the reduced mineral prepared by changing the hydrogen reaction time in Example 5. It is a graph which shows the change of the reducing power in the storage state of the reduced minerals prepared in Example 6. It is a process diagram of the confirmation test of the intracellular antioxidant activity using a living cell about the influence which the reducing property of a reducing mineral has on a living body in Example 7. FIG. It is a graph which shows the antioxidant activity in a living cell of the reduced mineral. It is a process diagram of the confirmation test of the intracellular sugar uptake activity in Example 8. It is a graph which shows the result of the confirmation test. It is a graph which shows the DPPH radical scavenging activity of the reduced mineral prepared by the method of this invention using each raw material of salt raw material, rock salt raw material, plant origin, and animal origin. It is a speculation diagram about the structure of the reduced mineral produced by the method of this invention. This is another example of the same speculation diagram. This is another example of the same speculation diagram.

Subsequently, an embodiment embodying the present invention will be described for understanding the present invention.
In the method for producing reduced minerals according to an embodiment of the present invention, as incineration ash, incineration ash of a plant produced by firing weeds and commercially available mangrove charcoal at about 900 ° C. (hereinafter referred to as plant incineration ash). )It was used.
A gas furnace with a capacity of 4 liters was used as an apparatus, and nitrogen gas and hydrogen gas were prepared.
The method for producing the reduced mineral according to the present embodiment is as follows.
After filling the gas furnace with nitrogen gas and putting the incineration ash into the gas furnace, the inside of the gas furnace is degassed so that the amount of hydrogen gas per 1 g of incineration ash is in the range of 1 to 1000 mM and the volume of the furnace. The gas furnace was filled with hydrogen gas and nitrogen gas so that the capacity of the hydrogen gas was 1.4 to 100% at room temperature and normal pressure. After performing the above treatments at room temperature and normal pressure, the gas furnace is heated so that the temperature is in the range of 600 to 1000 ° C. (preferably 700 to 900 ° C., more preferably 700 to 800 ° C.). Reduced minerals were prepared by reacting at a temperature maintained for 0.5 to 6 hours. After the temperature dropped to room temperature, exhaust gas was replaced with nitrogen, and the reduced calcined product of incinerated ash was recovered as a sample of reduced minerals.
In addition, the incinerated ash was heated in a nitrogen gas atmosphere, and the recovered ash was used as a control. As the water used for dissolution, MQ water prepared using an ultrapure water preparation device manufactured by Millipore was used. Although the DPPH used in the DPPH method was dissolved in ethanol in the present embodiment, it may be dissolved in acetonitrile.
In the present embodiment, the incineration ash is put into a gas furnace filled with nitrogen gas, but the gas furnace containing the incineration ash may be filled with nitrogen gas.
Hereinafter, more specific examples will be described with reference to the attached drawings.

(Example 1)
The DPPH radical scavenging activity (reducing power) of the prepared reduced mineral sample was investigated.
(Method)
The sample was prepared by heating the incineration ash in a hydrogen gas atmosphere (100% hydrogen) at a temperature of 800 ° C. for 4 hours. As a control, an incinerated ash prepared by heating in a nitrogen gas atmosphere (100% nitrogen) at 700 ° C. for 4 hours was used. Then, MQ water was used as a comparison.
The sample and control were dissolved in water to prepare a 60 mg / mL sample solution and a control solution.
As shown in the conditions of Table 1, 40 μL of each of the sample and control solutions and MQ water were mixed with 50 μL of 500 μMDPPH ethanol solution and 10 μL of 100 mM acetic acid solution, and left at room temperature for 30 minutes. Absorbance was measured at 520 nm. The capture rate was measured by the ratio of the absorbances of the sample solution, the control solution, and the MQ water. The result was the average value of the measurement of 4 samples. The obtained value was calculated as a Trolox conversion value measure value (Tolox Eq) based on the value obtained by measuring Trolox.

The results are shown in FIG.
As shown in FIG. 1, 40 μL of the reduced mineral solution (sample solution) reduced by hydrogen gas showed a reducing power corresponding to about 2.5 mM in terms of Trolox. That is, since the prepared reduced mineral solution contains 60 mg of mineral content per 1 mL, it can be said that it contains an extremely large amount of reducing ability, which is equivalent to about 1 mM per 1 mg of mineral.

(Example 2)
The change in reducing power with the preparation temperature was investigated.
The results of a study in which each temperature was changed in a hydrogen gas atmosphere for 4 hours are as follows.
(Method)
The sample was prepared by changing the temperature in the furnace from 500 ° C. to 1000 ° C. in 1 g of plant incineration ash by 100 ° C. and maintaining it in a hydrogen gas atmosphere for 4 hours.
The sample was dissolved in water to make a 60 mg / mL solution. The absorbance of the sample solution immediately after the solution was prepared and the sample solution 2 weeks after the solution was prepared were measured. Moreover, MQ water was used as a control solution.

Under the conditions for measuring the amount of reduction shown in Table 1, each sample solution was mixed with a 500 μM DPPH ethanol solution and left at room temperature for 30 minutes. Absorbance was measured at 520 nm. The capture rate was measured by the ratio of the absorbances of the sample solution and the control solution. The result was the average value of 4 measurements of each sample solution. The obtained value was calculated as a Trolox conversion value measure value based on the measured value of Trolox. The value is shown by the absorbance of the measured value. The results are shown in FIG. The white color of the bar graph indicates the measured value immediately after the sample solution was prepared, and the diagonal line indicates the measured value 2 weeks after the sample solution was prepared.

As shown in FIG. 2, the amount of DPPH radicals was reduced by the reduced minerals, but the amount of DPPH radicals scavenged by the reduced minerals was different depending on the temperature produced. It can be seen that it has no reducing property at 500 ° C., its activity is low at 600 ° C. or 1000 ° C., and it has a high reducing amount at 700 ° C. to 900 ° C. It was also confirmed that the activity was retained in the solution 2 weeks after the preparation of the sample solution.

(Example 3)
Next, using the ORAC measurement method, samples prepared by temperature were measured.
(Method)
Similar to Example 2, the reduced mineral sample was prepared by changing the temperature in the furnace from 500 ° C. to 1000 ° C. in 1 g of plant incineration ash by 100 ° C. and maintaining it in a hydrogen gas atmosphere for 4 hours. The sample was dissolved in water to make a 60 mg / mL solution. It was measured by the ORAC measurement method under the basic conditions shown in Table 2.

The results of the ORAC activity of the reduced mineral prepared by reacting in a hydrogen gas atmosphere for 4 hours using the temperature conditions shown in FIG. 3 showed the same tendency as the DPPH method. It can be seen that it has no reducing property at 500 ° C., its activity is low at 600 ° C., 900 ° C. or 1000 ° C., and it has a high reducing amount at 700 ° C. In addition, this result shows that 800 ° C. has the highest activity.

(Example 4)
The reducing power due to the change in the treated hydrogen concentration was measured.
Next, the change in the amount of reduction between the low hydrogen amount at the time of preparation and the mineral reduced product produced was examined.
(Method)
To prepare the sample, 1 g of incinerated plant ash was placed in a furnace, the temperature was set to 800 ° C., and the hydrogen gas concentration in the furnace was set to 0.7%, 1.4%, 2.8%, 5.5%, 11%, and 100%. It was created by maintaining the state for 4 hours. The sample was dissolved in water to make a 60 mg / mL sample solution. Moreover, MQ water was used as a control solution.
For the measurement of the amount of reduction, the sample solution and MQ water were mixed with a 500 μMDPPH solution under the conditions shown in Table 1 and left at room temperature for 30 minutes. Absorbance was measured at 520 nm. The capture rate was measured by the ratio of the absorbances of the sample solution and the control solution. The result was the average value of four measurements of the sample solution. The obtained value was calculated as a Trolox conversion value measure value based on the measured value of Trolox.

FIG. 4 is a diagram showing a change in the reducing power when the hydrogen concentration in the furnace of the reducing mineral is changed.
This time, as the reduced mineral, a reduced mineral prepared by reacting in a hydrogen gas atmosphere at a concentration indicated for 4 hours using a temperature condition of 800 ° C. was used, and the value is shown by absorbance.
Since the capacity of the gas furnace used this time is about 4 L, when the condition of 1% hydrogen gas is set, about 2 mM of hydrogen molecules are present in the furnace.
From this result, it was confirmed that a reduced product was produced even if the hydrogen concentration was set to 1.4%. Furthermore, it was confirmed that the amount of reduction increases depending on the gas concentration.

The following can be inferred from this result.
When the atomic weight of the reducing mineral is about 10 to 100 (most mineral atoms fall within this range), the equimolar amount of hydrogen required per 1 g of mineral is 10 to 100 mM. If you want to completely reduce minerals, even if equimolar hydrogen reacts, about 100 mM is required, and the gas furnace used this time requires about 50% of the amount. It should be possible to obtain fully reduced minerals by exchanging or supplying hydrogen gas if necessary, but this time it appears that the reducing activity is saturated at about 10%. Under these conditions, hydrogen reduction of 11% or more is not possible, or mutual reduction with minerals may cause a sufficient amount of reduction even with 10% hydrogen.

(Example 5)
The change in the reducing power of the reducing minerals prepared by changing the hydrogen reaction time was investigated.
Samples were prepared by changing the hydrogen reaction time. MQ water was used as the control solution.
(Method)
The sample was prepared by putting 1 g of incinerated plant ash into the furnace and maintaining the temperature at 800 ° C. and the hydrogen gas concentration in the furnace at 100% for each time. The sample was dissolved in water to make a 60 mg / mL solution.
For the measurement of the amount of reduction, the sample solution was mixed with the 500 μMDPPH solution under the conditions shown in Table 1 and left at room temperature for 30 minutes. Absorbance was measured at 520 nm. DPPH was dissolved in ethanol (or acetonitrile). The capture rate was measured by the ratio of the absorbances of the sample solution and the control solution. The result was the average value of the measurement of 4 samples. The obtained value was calculated as a Trolox conversion value measure value based on the measured value of Trolox.

FIG. 5 shows the results measured by the DPPH method of the reduced mineral prepared by reacting in a 100% concentration hydrogen gas atmosphere with the hydrogen reaction time as the notation time using the 800 ° C. temperature condition.
As shown in FIG. 5, the reduction was carried out even in the reaction for 0.5 hours, but the value was high in 1 to 4 hours. It was also confirmed that the activity was slightly reduced in 6 hours.

(Example 6)
The maintenance of the reducing activity of reduced minerals was investigated by DPPH radical scavenging activity.
Reduced mineral A created by reacting plant incineration ash at 800 ° C for 4 hours in a hydrogen gas atmosphere, reduced mineral B created by reacting plant incineration ash at 900 ° C for 4 hours in a hydrogen gas atmosphere And said. As a control, the same plant incineration ash as the sample was reacted at 700 ° C. for 4 hours in a nitrogen gas atmosphere, and the DPPH radical scavenging activity was examined under the same conditions as the sample. For comparison, the same measurement was performed for MQ water.
The sample was dissolved in water to prepare a 60 mg / mL solution.
(Method)
The sample solution was mixed with a 500 μMDPPH solution under the conditions shown in Table 1 and left at room temperature for 30 minutes. Absorbance was measured at 520 nm. The capture rate was measured by the ratio of the absorbances of the sample solution and the control solution. The result was the average value of four measurements of the sample solution. The obtained value was calculated as a Trolox conversion value measure value based on the measured value of Trolox. In addition, the same measurement was performed on the solutions 1 day, 7 days, and 1 month after each solution was prepared.

The results of the DPPH activity of the reduced mineral are shown in FIG.
In FIG. 6, the notations 1d, 7d and 1M refer to the sample solutions 1 day, 7 days and 1 month after the solution preparation, respectively.
As shown in FIG. 6, the reduced amount of the prepared reduced mineral was not changed even after at least one month had passed in the state of refrigerating and storing the prepared solution.

That is, the reduced mineral produced by the method of the present invention can maintain sufficient activity for one month even when stored under normal conditions not under anaerobic conditions, and further, in the case of refrigerated storage, after half a year. It was found that the activity remained.

From this result, the following can be inferred.
Normally, when minerals are reduced, they become hydrides or metals, but these do not maintain their reducing power because they immediately generate hydrogen and ionize in an aqueous solution. However, since the reduced mineral produced by the method of the present invention retains stable reducing activity even in an aqueous solution, it is presumed that a completely new mineral reduced product is produced.

(Example 7)
The intracellular antioxidant activity of reduced minerals was investigated.
In order to confirm whether the reducing property of the reducing mineral can affect the living body, the intracellular active oxygen scavenging activity was examined using HT1080 cells (human-derived fibroblastoma cell line) as cultured cells. .. The reduced mineral was prepared by reacting at 800 ° C. for 4 hours in a hydrogen gas atmosphere to prepare a sample, and the sample was dissolved in water to prepare a 60 mg / mL solution to prepare a sample solution.
(Method)
HT1080 cells are sprinkled twice in 96-well plates at a concentration of 0.5 × 105 cells / mL in 100 μL units, cultured for 24 hours, and then cultured in a medium containing 40 uM hydrogen peroxide for 30 minutes to damage the cells. Next, the medium was replaced with a sample solution-containing medium, and after culturing for 1 hour, a fluorescent reagent was treated to detect intracellular ROS (reactive oxygen species). In addition, instead of the sample solution-containing medium, an aqueous medium containing no reduced mineral was used, and the other medium treated in the same manner as the sample was used as a control. Details are shown in Fig. 7.
The results are shown in FIG. MEM is Eagle's minimum essential medium (cell culture medium by Eagle (1959)), PBS is phosphate buffered saline, and BES is equilibrium electrolyte.

As shown in FIG. 8, as a result of examining whether or not the sample can suppress the active oxygen generated in the cell using a living cell line, the reduced mineral was treated in the normal culture (A) without hydrogen peroxide treatment. However, when the amount of intracellular active oxygen is increased by (B) 40 μM hydrogen peroxide treatment and (C) 60 μM hydrogen peroxide treatment, treatment with reduced minerals depends on the amount of reduced minerals. It was confirmed that the amount of intracellular active oxygen was suppressed. This result suggests that reduced minerals can eliminate excess active oxygen generated in vivo.

(Example 8)
The intracellular sugar uptake activity was investigated.
In order to confirm whether the reducing power of reduced minerals can affect the living body, the cell sugar uptake activity was examined using cultured cells.
As the reduced mineral, a sample prepared by reacting at 800 ° C. for 4 hours in a hydrogen gas atmosphere was used. The sample was dissolved in water to prepare a 60 mg / mL solution, which was used as a sample solution. In addition, when the sample solution was treated into cells, it was diluted to 1/10 and 1/100 for treatment. The method of the confirmation test of the cell glucose uptake activity using the cultured cells (3T3-L1) is shown in FIG. 9, and the results are shown in FIG. DG represents diacylglycerol.

As shown in FIG. 10, when insulin stimulation is performed in MQ water, a clear increase in the glucose uptake rate can be confirmed. Treatment of reduced minerals diluted 1/10 times confirmed an increase in the glucose uptake rate even though insulin stimulation was not performed. Moreover, when the dilution is diluted to 1/100, the effect is lost. In the figure, MQ indicates MQ water, -ins indicates no insulin stimulation, and + ins indicates that insulin stimulation treatment was performed.
From these results, reduced minerals have the effect of promoting sugar uptake, and the use of reduced minerals can be expected to reduce insulin resistance occurring in diabetic patients.

Next, in order to explain the method of the present invention in more detail, Table 3 shows the results of analyzing the components of the plant incineration ash made from the weeds used in this example and commercially available mangrove charcoal.

The measurement was performed in the range measured by the elemental information of the raw material. Due to lot differences, there is some width.
Further, Table 4 shows the results of analyzing the components of the prepared reduced mineral solution.

As shown in Table 4, the components of the reduced mineral solution have a structure similar to that of bio-containing minerals. Since there are lot differences, there is a certain range.

Furthermore, the DPPH radical scavenging activity of the substance prepared by hydrogenating each incinerated ash derived from salt raw material, rock salt raw material, plant-derived, and animal (fish) by the method of the present invention was measured.
The result is shown in FIG. The numbers in parentheses are the dilution ratios of the samples.

As shown in FIG. 11, the DPPH radicals were not reduced in the hydrogen-treated rock salt and salt, and the scavenging activity was confirmed in the reduced minerals produced by ashing plants and fish. (Tolox is a positive control.)
From the above, in order to prepare the reduced mineral by the method of the present invention, it is considered essential to prepare the reduced mineral as a calcining material under a plurality of mineral conditions.
In the method of the present invention, incineration ash derived from a living body, that is, animal and / or plant is used as a raw material for the reduced mineral, but a powdery inorganic material having 85% or more of the components equivalent to the incineration ash is used. It is also possible.

Furthermore, in order to understand the method of the present invention, the structure of the reduced mineral produced by the method of the present invention was studied. Currently, there is no information on the structure, and it is speculated that it is important to have multiple of these minerals.
Currently, the following FIGS. 12, 13, and 14 can be considered as the inferred structures.

As shown in FIG. 12, when a metal is reduced, a minute metal body (nanoparticle) is generated by an electron, and a hydride is further produced. However, it is believed that they cannot be immediately dissolved and present in water. Therefore, a structure containing hydrogen as shown in FIG. 13 is conceivable, and the existence of a compound having this structure is known, but there is a problem that it does not have reducing activity. As for the reduced minerals in the method of the present invention, since a plurality of minerals are important, nanoparticles of a hydrogen storage alloy as shown in FIG. 14 are produced, or a novel hydride having low reactivity is generated. It is presumed that it has been done.

Claims (7)

The first step of putting incineration ash derived from one or both of plants and animals containing a plurality of minerals or a powdery inorganic substance having the same composition as the incineration ash into a gas furnace filled with nitrogen gas.
After the first step, the gas furnace is degassed, and the gas furnace is filled with hydrogen gas in the range of 1 to 1000 mM for 1 g of the incineration ash or the powdery inorganic substance.
The third step of heating the gas furnace to produce reduced minerals so that the temperature inside the furnace is in the range of 600 to 1000 ° C.
A method for producing a reduced mineral, which comprises a fourth step of recovering the reduced mineral by lowering the temperature in the furnace to room temperature, performing exhaust and nitrogen substitution after the third step. The first step of filling a gas furnace containing incineration ash derived from one or both of plants and animals containing a plurality of minerals or a powdery inorganic substance having the same composition as the incineration ash, and nitrogen gas.
After the first step, the gas furnace is degassed, and the gas furnace is filled with hydrogen gas in the range of 1 to 1000 mM for 1 g of the incineration ash or the powdery inorganic substance.
The third step of heating the gas furnace to produce reduced minerals so that the temperature inside the furnace is in the range of 600 to 1000 ° C.
A method for producing a reduced mineral, which comprises a fourth step of recovering the reduced mineral by lowering the temperature in the furnace to room temperature, performing exhaust and nitrogen substitution after the third step. The method for producing a reduced mineral according to claim 1 or 2, wherein the first step and the second step are carried out at normal temperature and pressure. In the method for producing a reduced mineral according to any one of claims 1 to 3, in the second step, the hydrogen gas is adjusted to 1.4 to 100% by volume at normal temperature and pressure with respect to the furnace capacity. , A method for producing a reduced mineral, which comprises filling only hydrogen gas or hydrogen gas and nitrogen gas. The method for producing a reduced mineral according to any one of claims 1 to 4, wherein the temperature inside the furnace in the third step is 700 to 900 ° C. The method for producing a reduced mineral according to any one of claims 1 to 5, wherein in the third step, the temperature inside the furnace is maintained for 0.5 to 6 hours. After incineration ash derived from one or both of plants and animals containing multiple minerals is placed in a gas furnace filled with nitrogen gas and degassed, hydrogen gas is added in the range of 1 to 1000 mM per 1 g of incineration ash. The gas furnace is heated so that the temperature inside the furnace is in the range of 600 to 1000 ° C. to prepare a reduced mineral, and the gas furnace is lowered to room temperature to be exhausted and replaced with nitrogen to recover the reduced mineral. A method for producing a reduced mineral having a characteristic intracellular sugar uptake activity.

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