JP6814132B2 - 内部タンパク質タグ - Google Patents

内部タンパク質タグ Download PDF

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Publication number
JP6814132B2
JP6814132B2 JP2017514354A JP2017514354A JP6814132B2 JP 6814132 B2 JP6814132 B2 JP 6814132B2 JP 2017514354 A JP2017514354 A JP 2017514354A JP 2017514354 A JP2017514354 A JP 2017514354A JP 6814132 B2 JP6814132 B2 JP 6814132B2
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JP
Japan
Prior art keywords
polypeptide
internal
protein
amino acid
seq
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Application number
JP2017514354A
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English (en)
Japanese (ja)
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JP2017533181A (ja
JP2017533181A5 (enExample
Inventor
アンドリュー エス ディクソン
アンドリュー エス ディクソン
ランス ピー エンセル
ランス ピー エンセル
トーマス マヒレイド
トーマス マヒレイド
マリー シュウィン
マリー シュウィン
キース ウッド
キース ウッド
モニカ ウッド
モニカ ウッド
クリス ツィマーマン
クリス ツィマーマン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
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Promega Corp
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Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of JP2017533181A publication Critical patent/JP2017533181A/ja
Publication of JP2017533181A5 publication Critical patent/JP2017533181A5/ja
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Publication of JP6814132B2 publication Critical patent/JP6814132B2/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y308/00—Hydrolases acting on halide bonds (3.8)
    • C12Y308/01—Hydrolases acting on halide bonds (3.8) in C-halide substances (3.8.1)
    • C12Y308/01005—Haloalkane dehalogenase (3.8.1.5)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
JP2017514354A 2014-09-12 2015-09-11 内部タンパク質タグ Active JP6814132B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462049875P 2014-09-12 2014-09-12
US62/049,875 2014-09-12
PCT/US2015/049741 WO2016040835A1 (en) 2014-09-12 2015-09-11 Internal protein tags

Publications (3)

Publication Number Publication Date
JP2017533181A JP2017533181A (ja) 2017-11-09
JP2017533181A5 JP2017533181A5 (enExample) 2018-10-18
JP6814132B2 true JP6814132B2 (ja) 2021-01-13

Family

ID=55459623

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017514354A Active JP6814132B2 (ja) 2014-09-12 2015-09-11 内部タンパク質タグ

Country Status (4)

Country Link
US (1) US9969991B2 (enExample)
EP (2) EP4219736A1 (enExample)
JP (1) JP6814132B2 (enExample)
WO (1) WO2016040835A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014236949C1 (en) 2013-03-15 2018-11-29 Promega Corporation Activation of bioluminescence by structural complementation
EP4219736A1 (en) * 2014-09-12 2023-08-02 Promega Corporation Internal protein tags
US11365402B2 (en) * 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
WO2019241438A2 (en) * 2018-06-12 2019-12-19 Promega Corporation Multipartite luciferase
JP2020080784A (ja) * 2018-11-29 2020-06-04 シスメックス株式会社 融合ポリペプチド、融合ポリペプチドの製造方法、及び融合ポリペプチドをコードするｄｎａ
EP3953374A4 (en) * 2019-04-10 2023-06-07 Promega Corporation COMPOSITIONS AND METHODS FOR DETECTION OF ANALYTES USING BIOLUMINESCENCE
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
AU2020347269A1 (en) * 2019-09-11 2022-03-31 Laboratory Corporation Of America Holdings Methods and systems for the rapid detection of microorganisms using recombinant infectious agents to express an indicator subunit
CN110592031B (zh) * 2019-09-25 2023-03-14 华南农业大学 一株S蛋白融合HiBiT的传染性支气管炎重组病毒及制备方法与应用
US20230211023A1 (en) 2020-03-25 2023-07-06 Erasmus University Medical Center Rotterdam Reporter system for radionuclide imaging
EP4519425A1 (en) * 2022-05-04 2025-03-12 Promega Corporation Modified dehalogenase with extended surface loop regions
AU2024265176A1 (en) * 2023-05-04 2025-12-11 Promega Corporation Complementation-based tags and reporters for dual-modality labeling

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5914095A (en) 1989-04-07 1999-06-22 Salutar, Inc. Polychelants containg amide bonds
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
WO1994009056A1 (en) 1992-10-14 1994-04-28 Sterling Winthrop Inc. Chelating polymers
GB9503969D0 (en) 1995-02-28 1995-04-19 Sams Bernard Incrementing mechanism
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
EP1931787A4 (en) * 2005-09-30 2010-10-20 Univ Montana State SYSTEM FOR DETECTING PROTEIN-PROTEIN INTERACTIONS
WO2010127368A1 (en) * 2009-05-01 2010-11-04 Promega Corporation Synthetic oplophorus luciferases with enhanced light output
SG10202103336SA (en) * 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
BR112015013710B1 (pt) * 2012-12-12 2022-08-09 Promega Corporation Métodos in vitro para detectar uma interação entre um agente bioativo e um alvo celular
AU2014236949C1 (en) * 2013-03-15 2018-11-29 Promega Corporation Activation of bioluminescence by structural complementation
EP4219736A1 (en) * 2014-09-12 2023-08-02 Promega Corporation Internal protein tags

Also Published As

Publication number Publication date
EP3191601A4 (en) 2018-04-04
EP3191601A1 (en) 2017-07-19
US9969991B2 (en) 2018-05-15
US20160097042A1 (en) 2016-04-07
WO2016040835A1 (en) 2016-03-17
JP2017533181A (ja) 2017-11-09
EP4219736A1 (en) 2023-08-02
EP3191601B1 (en) 2022-11-02

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