JP6802154B2 - 核酸シーケンシングデータを解析するための方法およびシステム - Google Patents

核酸シーケンシングデータを解析するための方法およびシステム Download PDF

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JP6802154B2
JP6802154B2 JP2017514334A JP2017514334A JP6802154B2 JP 6802154 B2 JP6802154 B2 JP 6802154B2 JP 2017514334 A JP2017514334 A JP 2017514334A JP 2017514334 A JP2017514334 A JP 2017514334A JP 6802154 B2 JP6802154 B2 JP 6802154B2
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locus
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sample
sequence
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ブリュアン ジョセリーン
ブリュアン ジョセリーン
ヨハン シュレンジャー フェリックス
ヨハン シュレンジャー フェリックス
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イラミーナ インコーポレーテッド
イラミーナ インコーポレーテッド
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/10—Ploidy or copy number detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B45/00—ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks

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  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Data Mining & Analysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2017514334A 2014-09-18 2015-09-15 核酸シーケンシングデータを解析するための方法およびシステム Active JP6802154B2 (ja)

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US201462052189P 2014-09-18 2014-09-18
US62/052,189 2014-09-18
PCT/US2015/050129 WO2016044233A1 (en) 2014-09-18 2015-09-15 Methods and systems for analyzing nucleic acid sequencing data

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JP2017528140A JP2017528140A (ja) 2017-09-28
JP2017528140A5 JP2017528140A5 (enExample) 2018-11-22
JP6802154B2 true JP6802154B2 (ja) 2020-12-16

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US (2) US20160085910A1 (enExample)
EP (1) EP3194627B1 (enExample)
JP (1) JP6802154B2 (enExample)
KR (1) KR102538753B1 (enExample)
CN (1) CN107002121B (enExample)
AU (1) AU2015318017B2 (enExample)
CA (1) CA2960840A1 (enExample)
SA (1) SA517381091B1 (enExample)
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Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3181696A1 (en) 2013-12-03 2015-06-11 Paul BELITZ Methods and systems for analyzing image data
US10600499B2 (en) 2016-07-13 2020-03-24 Seven Bridges Genomics Inc. Systems and methods for reconciling variants in sequence data relative to reference sequence data
US11001880B2 (en) 2016-09-30 2021-05-11 The Mitre Corporation Development of SNP islands and application of SNP islands in genomic analysis
CA3044231A1 (en) 2016-11-16 2018-05-24 Illumina, Inc. Validation methods and systems for sequence variant calls
US20200202982A1 (en) * 2017-05-17 2020-06-25 Syracuse University Methods and systems for assessing the presence of allelic dropout using machine learning algorithms
WO2019017806A1 (en) * 2017-07-20 2019-01-24 Huawei Technologies Co., Ltd APPARATUS AND METHOD FOR IDENTIFYING HAPLOTYPES
US11488009B2 (en) 2017-10-16 2022-11-01 Illumina, Inc. Deep learning-based splice site classification
MX2019014690A (es) 2017-10-16 2020-02-07 Illumina Inc Tecnicas basadas en aprendizaje profundo para el entrenamiento de redes neuronales convolucionales profundas.
US11861491B2 (en) 2017-10-16 2024-01-02 Illumina, Inc. Deep learning-based pathogenicity classifier for promoter single nucleotide variants (pSNVs)
EP4592893A3 (en) 2018-01-15 2025-08-20 Illumina, Inc. Deep learning-based variant classifier
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
NL2020861B1 (en) 2018-04-12 2019-10-22 Illumina Inc Variant classifier based on deep neural networks
CN108647492B (zh) * 2018-05-02 2019-04-16 中国人民解放军军事科学院军事医学研究院 一种染色质拓扑相关结构域的表征方法及装置
US12073922B2 (en) 2018-07-11 2024-08-27 Illumina, Inc. Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs)
WO2020028862A1 (en) * 2018-08-03 2020-02-06 Nantomics, Llc Panbam: bambam across multiple organisms in parallel
SG11201911777QA (en) 2018-10-15 2020-05-28 Illumina Inc Deep learning-based techniques for pre-training deep convolutional neural networks
US11521710B2 (en) 2018-10-31 2022-12-06 Tempus Labs, Inc. User interface, system, and method for cohort analysis
CN109920484A (zh) * 2019-02-14 2019-06-21 北京安智因生物技术有限公司 一种测序仪用的基因检测数据的分析方法及系统
WO2020191390A2 (en) 2019-03-21 2020-09-24 Illumina, Inc. Artificial intelligence-based quality scoring
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11705226B2 (en) 2019-09-19 2023-07-18 Tempus Labs, Inc. Data based cancer research and treatment systems and methods
CN110910960B (zh) * 2019-11-30 2022-07-08 浙江天科高新技术发展有限公司 一种鲍曼不动杆菌分子血清型快速分析方法
CN111292809B (zh) * 2020-01-20 2021-03-16 至本医疗科技（上海）有限公司 用于检测rna水平基因融合的方法、电子设备和计算机存储介质
CN111326213B (zh) * 2020-02-20 2023-10-03 苏州金唯智生物科技有限公司 一种数据分析方法、装置、设备及存储介质
EP4291680A4 (en) * 2021-02-12 2024-12-25 Verogen, Inc. METHODS AND COMPOSITIONS FOR DNA-BASED KINSHIP ANALYSIS
EP4416734A4 (en) * 2021-10-13 2025-08-27 Verogen Inc METHODS AND COMPOSITIONS FOR IMPROVING THE ACCURACY OF DNA-BASED KINSHIP ANALYSIS
CN114420208B (zh) * 2022-02-28 2023-04-18 上海亿康医学检验所有限公司 一种用于鉴定核酸样本中cnv的方法和装置
CN119920309B (zh) * 2025-04-03 2025-07-04 中国农业科学院农业基因组研究所 基于邻近连接Motif序列的Hi-C数据质量快速评估方法Motif-Hi-C及应用

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
AU3539089A (en) 1988-04-08 1989-11-03 Salk Institute For Biological Studies, The Ligase-based amplification method
ATE144556T1 (de) 1988-06-24 1996-11-15 Amgen Inc Verfahren und mittel zum nachweis von nukleinsäuresequenzen
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
ES2089038T3 (es) 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
WO1995021271A1 (en) 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
DK1801214T3 (da) 1997-07-07 2011-01-24 Medical Res Council In vitro sorteringsfremgangsmåde
US6238863B1 (en) * 1998-02-04 2001-05-29 Promega Corporation Materials and methods for indentifying and analyzing intermediate tandem repeat DNA markers
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274317B1 (en) * 1998-11-02 2001-08-14 Millennium Pharmaceuticals, Inc. Automated allele caller
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US6807490B1 (en) * 2000-02-15 2004-10-19 Mark W. Perlin Method for DNA mixture analysis
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
JP2004513619A (ja) 2000-07-07 2004-05-13 ヴィジゲン　バイオテクノロジーズ　インコーポレイテッド リアルタイム配列決定
WO2002008469A2 (en) * 2000-07-21 2002-01-31 Applera Corporation Methods, systems, and articles of manufacture for evaluating biological data
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7272506B2 (en) * 2002-03-01 2007-09-18 Applera Corporation Computer algorithm for automatic allele determination from fluorometer genotyping device
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
SI3363809T1 (sl) 2002-08-23 2020-08-31 Illumina Cambridge Limited Modificirani nukleotidi za polinukleotidno sekvenciranje
CA2513535C (en) 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
US20040215401A1 (en) * 2003-04-25 2004-10-28 Krane Dan Edward Computerized analysis of forensic DNA evidence
WO2005010145A2 (en) 2003-07-05 2005-02-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
US7127355B2 (en) * 2004-03-05 2006-10-24 Perlegen Sciences, Inc. Methods for genetic analysis
WO2006044078A2 (en) 2004-09-17 2006-04-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
JP4922646B2 (ja) * 2005-12-14 2012-04-25 株式会社日立ソリューションズ 遺伝子情報の表示方法及び表示装置
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
SG170802A1 (en) 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
WO2011139797A2 (en) * 2010-04-27 2011-11-10 Spiral Genetics Inc. Method and system for analysis and error correction of biological sequences and inference of relationship for multiple samples
WO2013052557A2 (en) * 2011-10-03 2013-04-11 Natera, Inc. Methods for preimplantation genetic diagnosis by sequencing
CA3207599A1 (en) * 2010-05-18 2011-11-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
WO2012092515A2 (en) * 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
BR112013020220B1 (pt) * 2011-02-09 2020-03-17 Natera, Inc. Método para determinar o estado de ploidia de um cromossomo em um feto em gestação
DK2766496T3 (en) * 2011-10-11 2017-05-15 Sequenom Inc METHODS AND PROCESSES FOR NON-INVASIVE ASSESSMENT OF GENETIC VARIATIONS
US20140310025A1 (en) * 2011-11-21 2014-10-16 Advanced Biological Laboratories Sa Systems, methods, and computer program products for guiding the selection of therapeutic treatment regiments
US20130261984A1 (en) * 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
US20140163900A1 (en) * 2012-06-02 2014-06-12 Whitehead Institute For Biomedical Research Analyzing short tandem repeats from high throughput sequencing data for genetic applications
EP3418395A1 (en) * 2012-09-06 2018-12-26 Life Technologies Corporation Multiplex y-str analysis
CA2890441A1 (en) * 2012-11-07 2014-05-15 Good Start Genetics, Inc. Methods and systems for identifying contamination in samples
WO2014116729A2 (en) * 2013-01-22 2014-07-31 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of hla loci with ultra-deep shotgun sequencing
AU2013382195B2 (en) 2013-03-13 2019-09-19 Illumina, Inc. Methods and systems for aligning repetitive DNA elements

Also Published As

Publication number Publication date
EP3194627B1 (en) 2023-08-16
EP3194627A1 (en) 2017-07-26
KR102538753B1 (ko) 2023-05-31
EP3194627C0 (en) 2023-08-16
EP3194627A4 (en) 2018-05-30
AU2015318017B2 (en) 2022-02-03
WO2016044233A1 (en) 2016-03-24
US20210375396A1 (en) 2021-12-02
AU2015318017A1 (en) 2017-03-30
JP2017528140A (ja) 2017-09-28
US20160085910A1 (en) 2016-03-24
CA2960840A1 (en) 2016-03-24
SA517381091B1 (ar) 2021-03-31
CN107002121A (zh) 2017-08-01
CN107002121B (zh) 2020-11-13
KR20170056682A (ko) 2017-05-23

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