JP6771229B2 - Needle support fixture and needle device - Google Patents

Description

The present invention relates to a needle support fixture that supports and fixes an injection needle when injecting a drug into a tissue. Further, the present invention relates to an injection needle device having an injection needle supported and fixed by an injection needle support fixture, specifically, a perfusion needle device used when injecting a perfusate into the heart of a laboratory animal. Regarding.

When injecting a perfusate into a laboratory animal to proceed with the experimental protocol, the perfusate may be injected into the heart of the laboratory animal. For example, in perfusion fixation of a rat, the rat is anesthetized with an anesthetic and an incision is made in the chest to make the heart visible. Then, for example, holding an injection needle device in the left hand and a hookless forceps in the right hand, the injection needle is inserted into the left ventricle from the apex of the heart, and the needle tip is inserted upward. Fix the injection needle together with the heart with a hookless forceps so that the injection needle does not come off. Phosphate buffered saline is flushed, then the fixative is perfused, and when the animal's body becomes stiff, the tissue is removed and stored in the fixative (Non-Patent Documents 1 and 2).

Perfusion fixation requires rapid injection of perfusate to fix the tissue because the central nervous system is susceptible to postmortem changes, and if the experimental animal is a small individual, especially during the embryonic period or shortly after birth. The heart that punctures the injection needle is small, and for these reasons, perfusion fixation and the like may not be performed properly. For example, an erroneous needle that intends to insert the needle tip into the left ventricle but punctures a place other than the left ventricle, or, for example, after puncturing the left ventricle with an injection needle, the tip of the injection needle is appropriate in the ventricle due to penetration of the injection needle. There are problems such as excessive puncture that is not located in a place, and for example, the punctured injection needle comes off because the injection needle is not properly fixed.

Such perfusion-fixation failures can be reduced by training, but cannot be completely avoided. Especially for beginners, it is extremely difficult to completely control these problems, and the burden on laboratory animals and workers is great. It can be said that it is extremely significant in life science research that even beginners can easily and accurately perfuse the heart of experimental animals.

Therefore, there is a method of bending the middle of the injection needle at an angle of about 45 to 60 degrees in advance. According to such a method, the bent portion of the injection needle serves as a mark, the length of the injection needle invading the heart can be easily grasped, and the bent portion of the injection needle is hooked on the inner wall of the heart. It becomes easier to fix. However, bending the injection needle may damage the injection needle, and the flow path at the bent portion of the injection needle may be narrowed so that the perfusate cannot be properly injected. Furthermore, even if the bent portion of the injection needle is hooked on the inner wall of the heart, the degree of engagement is weak, and it is conceivable that the injection needle will come off when a certain amount of force is applied.

On the other hand, Patent Document 1 describes an injection needle device including an injection needle body and a needle position defining member. The injection needle body has an injection needle, a positioning stopper that abuts on the needle position defining member, and a return member that is provided on the side surface of the injection needle and projects toward the oblique proximal end side. The needle position defining member has a rubber member through which the injection needle can penetrate, and when the injection needle penetrates the rubber member and the positioning stopper abuts on a predetermined position of the needle positioning member and is positioned, the return member. Engages the rubber member.

The invention described in Patent Document 1 is an injection needle device used when puncturing human skin and injecting a drug solution, and the return member for preventing the injection needle from slipping does not damage human skin more than necessary. In addition, the injection needle device described in Patent Document 1 has a large number of parts because it engages with a rubber member. In this way, an injection needle that injects a drug solution into human skin is required to have a configuration that emphasizes safety even if the number of parts is increased. For example, an injection needle that injects a perfusate solution into the heart of an experimental animal Since the safety of the animal to be injected is not required, but rather a simple and reliable injection of the perfusate is required, an increase in the number of parts in the injection needle device for perfusion of experimental animals is such a request. To prevent. Further, the needle position defining member in the invention described in Patent Document 1 becomes a relatively large part when the purpose is to fix the injection needle, and it is difficult to divert it to a case where the surgical part is small such as cardiac perfusion in an experimental animal. is there. Further, the invention described in Patent Document 1 is intended for a dry and flat portion such as skin, and it is difficult to accurately fix and use it on a wet and small curved surface such as the heart of a small animal. Is.

JP-A-2009-233286

Jung-Hwa, T. ao-C. Heng, Gallant, J., Brightman, PE, Dosemeci, MW, A ,, Reese, TS Structural changes at the synapse after delayed perfusion fixation in different regions of the mouse brain. J. Comp. Neurol. 501, 731-740 (2007). Surgical method for rats, Toshihiko Katabuchi, Japanese Physiology Magazine Vol.63, No.10 2001.

The present invention has been made in view of such a problem, and an object of the present invention is to provide an injection needle support fixture capable of easily and surely injecting a drug into a tissue.

The injection needle support fixture according to the present invention is an injection needle support fixture that supports and fixes the injection needle when injecting a drug into a tissue, and an injection that exposes the needle tip of the injection needle to support the injection needle. It is characterized by having a needle support portion and an injection needle fixing portion for fixing an injection needle punctured by the tissue by being attached to the tissue.

The injection needle device according to the present invention is an injection needle device for injecting a drug into a tissue, and is an injection needle support fixture for supporting and fixing the injection needle and an injection supported by the injection needle support fixture. The injection needle support fixture includes a needle, and the injection needle support fixture includes an injection needle support portion that exposes the needle tip of the injection needle to support the injection needle, and an injection needle that is attached to the tissue and pierced into the tissue. It is characterized by having an injection needle fixing portion for fixing.

According to the present invention, the drug can be easily and surely introduced into the tissue. For example, when introducing the perfusate into the heart of an experimental animal, the perfusate can be easily and surely introduced into the heart of the animal.

It is a top view explaining the outline of the injection needle support fixture which concerns on Embodiment 1. FIG. It is a side view explaining the outline of the injection needle support fixture which concerns on Embodiment 1. FIG. It is a top view explaining the state which supported the injection needle by the injection needle support fixture which concerns on Embodiment 1. FIG. It is a top view explaining the state which supported the injection needle by the injection needle support fixture which concerns on Embodiment 2. FIG. It is a side view explaining the state which supported the injection needle by the injection needle support fixture which concerns on Embodiment 2. FIG. FIG. 5 is a plan view illustrating an injection needle support fixture having a configuration in which a clip is attached to a cylinder spacer with a spirally wound metal wire member. It is a top view explaining the usage mode of introducing a perfusate into the heart of a laboratory animal. It is a side view explaining the usage mode of introducing a perfusate into the heart of a laboratory animal. FIG. 5 is a plan view illustrating a usage mode for introducing a perfusate into the heart of a laboratory animal using a needle support fixture provided with a spirally wound metal wire member. It is a side view explaining the outline of the injection needle instrument which has an injection needle including a return member. It is sectional drawing explaining the injection needle instrument which has the injection needle which includes the return member. It is a photograph figure of the injection needle support fixture which concerns on this Example. It is a photographic figure explaining the state which attaches the injection needle to the injection needle support fixture which concerns on this Example. It is a photographic figure explaining the state which supported the injection needle by the injection needle support fixture which concerns on this Example. It is a photograph figure of the injection needle device which concerns on this Example.

Hereinafter, embodiments of the present invention will be specifically described with reference to the accompanying drawings, but the embodiments are for facilitating understanding of the principles of the present invention, and the scope of the present invention is as follows. The present invention is not limited to the embodiment, and other embodiments in which those skilled in the art appropriately replace the configurations of the following embodiments are also included in the scope of the present invention.

(Embodiment 1)
FIG. 1 is a plan view illustrating an outline of the injection needle support fixture 800 according to the present embodiment. The needle support fixture 800 supports and fixes the needle when injecting the drug into the tissue. For example, the needle support fixture 800 is used to support and secure the needle when introducing perfusate into the left ventricle of the experimental animal heart.

As shown in FIG. 1, the injection needle support fixture 800 is attached to a tissue by being attached to a plate portion 240 as an injection needle support portion for supporting the injection needle by exposing the needle tip of the injection needle. It has a clip 300 as an injection needle fixing portion for fixing the punctured injection needle. The needle fixation is attached, for example, to the heart wall of the left or right ventricle of the experimental animal heart.

The plate portion 240 is composed of a single plate portion, and has a substantially rectangular shape in a plan view. The plate portion 240 is provided with a valley fold line 250 that divides the plate portion 240 into a first plate portion 220 and a second plate portion 230 at a position connecting the midpoints of the short sides. The plate portion 240 and the clip 300 are connected by a connecting portion 361.

FIG. 2 is a side view illustrating the outline of the injection needle support fixture 800 according to the present embodiment. As shown in FIG. 2, the first plate portion 220 and the second plate portion 230 have a first adhesive surface 221 and a second adhesive surface 231 that are detachably adhered to each other on opposite inner surfaces. The first adhesive surface 221 and the second adhesive surface 231 are not particularly limited, but are, for example, flexible adhesive members, specifically urethane adhesive members. The first adhesive surface 221 and the second adhesive surface 231 are covered with a seal portion (not shown), and the first adhesive surface 221 and the second adhesive surface 231 are exposed by peeling off the seal portion. A part of the first plate portion 220 extends toward the clip 300 side to form a connecting portion. The connecting portion connects, for example, the first plate portion 220 and one knob portion 320 of the clip 300.

FIG. 3 is a plan view illustrating a state in which the injection needle support fixture 800 according to the present embodiment supports the injection needle 100 having the needle tip 110. The injection needle device 900 is a device for injecting a drug into a tissue, and includes an injection needle support fixture 800 and an injection needle 100 supported by the injection needle support fixture 800.

The injection needle 100 is placed so as to be attached to the first adhesive surface 221 of the first plate portion 220, the first plate portion 220 and the second plate portion 230 are folded along the valley fold line 250, and the first plate portion 220 is the first. The needle tip 110 of the injection needle 100 is exposed and the injection needle 100 is sandwiched between the adhesive surface 221 and the second adhesive surface 231 of the second plate portion 230 to adhesively support the injection needle 100.

The injection needle 100 is not particularly limited, but is, for example, an injection needle that introduces a perfusate into the left ventricle of a laboratory animal. Such an injection needle is not particularly limited as long as it can introduce a perfusate into the left ventricle even in the case of a small individual such as in the embryonic period or immediately after birth, and the diameter is, for example, 0.3 mm. Those having a diameter of about 0.7 mm and a length of 20 mm to 30 mm can be used, and more preferably, those having a diameter of 0.4 mm and a length of 25 mm can be used. The base end of the injection needle 100 is attached to a liquid delivery tube that feeds the perfusate.

For example, in the usage mode in which the injection needle used for introducing the reflux fluid into the heart of an experimental animal is supported by the injection needle support fixture 800, the first plate portion is used when the injection needle 100 is punctured into the heart. The short side of the 220 and the second plate 230 on the needle tip 110 side abuts on the apex-side outer wall of the left ventricle of the heart. The length of the exposed portion of the injection needle 100 sandwiched between the first plate portion 220 and the second plate portion 230 is smaller than the length of the outer wall on the apex side and the inner wall on the heart base side of the left ventricle. For example, the length of the exposed portion of the injection needle 100 sandwiched between the first plate portion 220 and the second plate portion 230 can be 3.0 mm to 5.0 mm, and is preferably 4.0 mm. The heart wall forming the internal space of the left ventricle has an interventricular septum, a side wall of the left ventricle, and a lower wall of the left ventricle on the apex side (lower side). The side wall of the left ventricle has an anterior wall of the left ventricle, a posterior wall of the left ventricle, and a wall of the left ventricle located opposite the interventricular septum. The heart wall that forms the interior space of the right ventricle is defined in the same way as the left ventricle.

In the above configuration, the plate portion 240 is composed of a single plate portion, but the present invention is not limited to such an embodiment. For example, the plate portion 240 is a separate body. It is also possible to include one plate portion 220 and a second plate portion 230.

(Embodiment 2)
As shown in FIG. 4, the injection needle support fixture 800 is attached to a tissue and a cylinder spacer 200 as an injection needle support portion that exposes the needle tip 110 of the injection needle 100 and supports the injection needle 100. It has a clip 300 as an injection needle fixing portion for fixing the injection needle 100 punctured into the tissue. The injection needle device 900 includes an injection needle support fixture 800 and an injection needle 100 supported by the injection needle support fixture 800.

The cylinder spacer 200 includes a hollow cylinder that is concentrically attached around the injection needle 100. By inserting the injection needle 100 into the hollow inside of the cylinder, the injection needle 100 is supported by friction between the outer surface of the injection needle and the inside of the hollow of the cylinder.

For example, in the usage mode in which the injection needle used for introducing the reflux fluid into the heart of a laboratory animal is supported by the injection needle support fixture 800, the cylinder spacer 200 is used when the injection needle 100 is punctured into the heart. , The tip 210 of the cylinder abuts on the apex-side outer wall of the left ventricle of the heart. As will be described later, in the cylinder spacer 200, the length of the needle tip of the injection needle 100 and the tip of the cylinder is smaller than the length of the outer wall of the left ventricle on the apex side and the inner wall of the heart base side. For example, the length between the tip of the injection needle 100 and the tip of the cylinder can be 3.0 mm to 5.0 mm, preferably 4.0 mm. For example, since the injection needle device 900 is used to introduce the perfusate into the left ventricle, the length between the needle tip of the injection needle 100 and the tip of the cylinder is the apex of the left ventricle. It is longer than the wall thickness on the part side.

FIG. 5 is a side view illustrating the outline of the injection needle device 900. The clip 300 grips the side wall of the ventricle. The side wall of the ventricle gripped by the clip 300 may be either the side wall of the right ventricle or the side wall of the left ventricle. In the configurations shown in FIGS. 4 and 5, the clip 300 grips the side wall of the left ventricle. In the clip 300, a pressure contact portion 310 is formed at the tip portion and a knob portion 320 is formed at the rear end portion, and each of the clip 300 is pressed against a pair of arm members 340 pivotally supported by the pivot portion 330 at a substantially intermediate position by elastic force. It has an elastic portion 350 that is urged to bring the portions 310 closer to each other and grips the side wall of the ventricle.

The clip 300 is attached to the side of the cylinder spacer 200 by an attachment portion 360, which is a hollow cylinder that is concentrically attached around the cylinder spacer 200. The attachment portion 360 and the pivot portion 330 are connected by a connection portion 361.

The pressure contact portion 310 is formed with a plurality of streaky uneven portions 311 on surfaces facing each other. This makes it possible to reduce slippage and properly grip the side wall of the ventricle. It is also possible to apply an insoluble antislip agent to the pressure contact portion 310. As the antislip agent, resins such as polyamide-based, polyester-based, polyolefin-based, synthetic rubber-based, acrylic-based, urethane-based, and epoxy-based resins can be used.

The perfusate that can be introduced by the injection needle device 900 is not particularly limited, and for example, a phosphate buffered saline solution, a paraformaldehyde aqueous solution, a glutaaldehyde aqueous solution, or the like can be used. Further, the experimental animal is not particularly limited, and for example, mice, rats, guinea pigs, rabbits, dogs and the like can be used.

In the above-described embodiment, the clip 300 includes a pair of arm members 340 having a pressure contact portion 310, a knob portion 320, and a pivot portion 330, and an elastic portion 350 that urges each pressure contact portion 310 in a direction of approaching each other. However, the clip 300 is not limited to such a configuration, and various configurations can be adopted. For example, the clip 300 is elastically urged in the direction of expansion, and a pair of arm members that grip the side wall of the ventricle, and a pair of arms that are fitted into the pair of arm members and move toward the tip end side. It is possible to have a configuration having a ring that keeps the member forcibly closed.

Further, in the above-described embodiment, the clip 300 is attached to the cylinder spacer 200 by the attachment portion 360 which is a hollow cylinder, but the configuration in which the clip 300 is attached to the cylinder spacer 200 is not limited to such a configuration. .. Various structures can be adopted as long as the clip 300 is attached to the side portion of the cylinder spacer 200. FIG. 6 is a plan view illustrating the outline of the injection needle device 900 having another configuration in which the clip 300 is attached to the cylinder spacer 200. As shown in FIG. 6, it is also possible to form a metal wire member 370 extending from the pivot portion 330 and spirally wound and attached around the cylinder spacer 200. In the configuration shown in FIG. 6, the clip 300 grips the side wall of the right ventricle.

Next, a specific example of the usage mode of the injection needle device 900 having the above configuration will be described. FIG. 7 is a plan view illustrating a usage mode in which a perfusate is introduced into a heart 700 of a laboratory animal using an injection needle device 900.

As shown in FIG. 7, the ventricle of the experimental animal heart 700 is divided into a left ventricle 710 and a right ventricle 720 by an interventricular septum 730. The apex 740 is located on the lower edge of the heart 700 and the heart base 750 is located on the upper edge.

First, the needle tip 110 of the injection needle 100 is punctured into the left ventricle 710 from the apex 740 side. When the injection needle 100 is inserted in the needle stick direction, the tip 210 of the cylinder spacer 200 comes into contact with the outer wall of the left ventricle 710 on the apex 740 side. The length X of the needle tip 110 of the injection needle 100 and the tip 210 of the cylinder is greater than the length Y of the outer wall of the left ventricle of the animal heart on the apex side and the inner wall of the left ventricle on the heart base side. small.

FIG. 8 is a side view illustrating a usage mode for introducing a perfusate into the heart 700 of a laboratory animal using a needle device 900. As shown in FIG. 8, after the tip 210 of the cylinder spacer 200 abuts on the outer wall of the left ventricle 710 on the apex 740 side, the knob 320 is pinched to open the pressure contact 310 and grip the side wall of the left ventricle. To do. In the configuration shown in FIGS. 7 and 8, the pressure contact portion 310 grips the side wall end portion of the left ventricle 710. That is, in the configurations shown in FIGS. 7 and 8, one arm member of the clip abuts on the side wall end on the anterior surface side of the left ventricle, and the other arm member abuts on the side wall end on the posterior surface side of the left ventricle. Is gripping the left ventricle. If the clip grips the central part of the side wall of the left ventricle, the clip may press on the inside of the left ventricle and prevent the smooth inflow of perfusate, and the clip may impede the smooth inflow of the perfusate. This is because by gripping the end portion, a good visibility to the punctured portion of the user of the injection needle device 900 can be ensured.

The length X of the needle tip 110 of the injection needle 100 and the tip 210 of the cylinder is greater than the length Y of the outer wall of the left ventricle of the animal heart on the apex side and the inner wall of the left ventricle on the cardiac base side. Since it is small, it is possible to prevent the needle tip 110 of the injection needle 100 from penetrating the inner wall of the left ventricle on the heart base side. Further, since the clip 300 grips the side wall of the ventricle, the injection needle 100 can be accurately fixed. As a result, the perfusate can be introduced easily and reliably.

Next, a specific example of the usage mode of the injection needle device 900 having a different configuration in which the clip 300 is attached to the cylinder spacer 200 in the spirally wound metal wire member 370 will be described. FIG. 9 is a plan view illustrating a usage mode for introducing a perfusate into the heart 700 of a laboratory animal using an injection needle device 900 including a spirally wound metal wire member 370.

In the configuration shown in FIG. 9, the pressure contact portion 310 grips the substantially central portion of the side wall of the right ventricle 720. That is, in the configuration shown in FIG. 9, one arm member of the clip abuts on the substantially central portion of the side wall on the anterior surface side of the right ventricle, and the other arm member abuts on the substantially central portion of the side wall on the posterior surface side of the right ventricle. Holds the right ventricle. When the clip grips the left ventricle, it is necessary to consider avoiding compression of the left ventricle by the clip, so it is preferable that the clip grips the side wall end of the left ventricle, but the clip grips the right ventricle. In the case of gripping the right ventricle, even if compression of the inside of the right ventricle by the clip occurs, the smooth inflow of the perfusate is not hindered, so that the clip can grip the substantially central portion of the side wall of the right ventricle. Since it is easier to work by gripping the substantially central portion of the side wall of the right ventricle than by gripping the end of the side wall of the left ventricle, the clip 300 grips the substantially central portion of the side wall of the right ventricle 720 by the pressure welding portion 310. Is preferable.

(Embodiment 3)
In the above-described embodiment, the injection needle fixing portion for fixing the injection needle is the clip 300, but in the third embodiment, the injection needle fixing portion is a return member 400 provided on the side portion of the injection needle 100.

FIG. 10 is a side view illustrating the outline of the injection needle device 900 according to the second embodiment. As shown in FIG. 10, a return member 400 is provided on the side portion of the injection needle 100 so as to project obliquely to the rear side. The return member 400 is integrally formed of the same material as the injection needle.

The needle tip 110 of the injection needle 100 of the injection needle device 900 of the present configuration is punctured into the left ventricle 710 from the apex 740 side, and the tip 210 of the cylinder spacer 200 is brought into contact with the outer wall of the left ventricle 710 on the apex 740 side. .. After that, even if an unexpected force is applied and the injection needle instrument 900 is pushed in the extraction direction, the return member 400 engages with the inner wall of the left ventricle 710 on the apex 740 side and is caught, so that the injection needle 100 is caught. It can be prevented from coming off.

The return member 400 is preferably elastically urged in a direction in which the rear portion is separated from the injection needle 100. As a result, when the injection needle 100 is inserted into the left ventricle 710, the return member 400 abuts on the wall on the apex 740 side of the left ventricle 710 and is folded to minimize damage to the wall of the left ventricle 710. The return member 400 expands after passing through the wall of the left ventricle 710, so that the return member 400 is on the apex 740 side of the left ventricle 710 even if the injection needle device 900 is pushed in the removal direction. It engages with the inner wall of the heart and gets caught.

Although only one return member 400 is provided in FIG. 10, a plurality of return members 400 can be provided on the side portion of the injection needle 100. Further, the return member 400 is not limited to the configuration of being integrally formed of the same material as the injection needle, and as shown in FIG. 11, the return member 400 may be integrally formed of the same material as the cylinder spacer 200. It is possible. In the case of this configuration, the cylinder spacer 200 and the return member 410 can be integrally formed by cutting a hollow cylinder concentrically attached around the injection needle 100.

According to the configuration of the third embodiment, the perfusate can be introduced into the heart with a simpler configuration, although the fixed surface of the injection needle 100 is slightly inferior to the configurations of the first and second embodiments. In the first and second embodiments, the injection needle fixing portion is the clip 300, and in the third embodiment, the return members 400 and 410 are used. However, it is also possible to adopt a configuration including both of these embodiments.

(Example 1)
As shown in FIG. 12, an injection needle support fixture having a plate portion as an injection needle support portion and a clip as an injection needle fixing portion was prepared. The plate part was divided into a first plate part and a second plate part by a valley fold line. Both the first plate portion and the second plate portion were made of polypropylene, and each had a rectangular shape in a plan view, a short side of 5 mm, a long side of 15 mm, and a thickness of 0.7 mm. Both the first adhesive surface and the second adhesive surface were formed of double-sided tape having a urethane adhesive.

As shown in FIG. 13, the injection needle was placed on the first adhesive surface of the prepared injection needle support fixture. The injection needle was Terumo 27G. As shown in FIG. 14, the first plate portion and the second plate portion are folded along the valley fold line, and the injection needle is inserted between the first adhesive surface of the first plate portion and the second adhesive surface of the second plate portion. It was pinched and adhesively supported by the injection needle. The needle tip of the injection needle was exposed, and the length of the portion of the injection needle exposed from the adhesively supported plate portion was 4.0 mm. The length of this exposed part of the injection needle is smaller than the length of the apical outer wall of the left ventricle of the animal heart and the inner wall of the left ventricle on the base side.

(Example 2)
As shown in FIG. 15, an injection needle support fixture having a cylinder spacer which is a hollow cylinder as an injection needle support portion and a clip as an injection needle fixing portion is created, and an injection needle (27G manufactured by Terumo) is used. A syringe needle device was created in support. A plastic tube with a length of 14.5 mm was used as the cylinder spacer. The clip as an injection needle fixing portion has a pressure contact portion formed at the tip portion and a knob portion formed at the rear end portion, and a pair of arm members pivotally supported at an intermediate position and each pressure contact portion approach each other by elastic force. It was a clothespin-shaped clip having an elastic part for urging in the squeezing direction. The length X between the needle tip and the tip of the cylinder was 4.0 mm. The clip was attached to the cylinder spacer with a spirally wound metal wire member. The clip gripped the side wall of the right ventricle.

Formalin perfusion fixation (hereinafter referred to as perfusion fixation) of mice was performed using an existing needle which is a conventional example and an injection needle device according to this example. The examiner was a second-year graduate master's student who was a beginner with no experience in perfusion. The existing needle was Terumo 27G.

Other instruments and reagents used were perista pump, dissecting scissors, tweezers, forceps, stainless bat, cork plate, insect pin, 10% buffered formalin (made by Nacalai Tesque), phosphate buffered saline (made by Nacalai Tesque), and pentovalbital (50 mg /). ml, made by Dynabot).

The mice used were 8-week-old male C57BL / 6 strain mice, and 10 mice were used.

The perfusion fixation method will be described below. Mice were intraperitoneally administered with pentobarbital 50 mg / kg, deeply anesthetized, and then doggy-style fixed on a cork plate. The chest cavity was opened to expose the heart. In the case of an existing injection needle, the needle tip was pierced into the left ventricle and the needle was held by hand.

On the other hand, in the injection needle device according to the present embodiment, the needle tip of the injection needle is punctured into the left ventricle from the apex side, and the tip of the cylinder spacer is brought into contact with the outer wall of the left ventricle on the apex side. Was properly installed so that it was located in the left ventricle.

The right atrium was incised and 10 mL of PBS was perfused at a rate of 1 mL / min using a perista pump. Then, the perfusate was replaced with a 10% buffered formalin solution, and another 15 mL was perfused.

During this period, we evaluated events that could be indicators of successful perfusion, such as the presence or absence of technical problems such as accidental puncture, needle omission during perfusion, and excessive puncture, liver blood loss, leakage of perfusate from the nasal cavity, and presence or absence of formalin-induced convulsions. did. After perfusion, the hardening of the body was evaluated, and the brain was removed and observed.

A simple technical instruction was given to the examiner for perfusion fixation of mice, and 5 mice were perfusion-fixed with the heart using the existing injection needle and the injection needle device according to this example. The results are shown in Table 1 below.

As shown in Table 1, as a result of verifying the frequency of problem cases such as erroneous puncture during work, needle omission during perfusion, and piercing too deeply, all the events with existing injection needles are 1 ■ 3 out of 5 cases. It happened in the example. On the other hand, when the injection needle device of this example was used, such a problematic event was not observed. For these events, especially if two or more occur together, it is considered that the final failure of perfusion fixation will occur. By using the injection needle device according to the present invention, it is possible to almost eliminate experimental mistakes that lead to failure of perfusion fixation.

A common evaluation point for whether perfusion is proceeding correctly is whitening due to hepatic blood loss. The liver, which has many capillaries and contains a large amount of blood, loses red blood cells and changes from dark red to light brown when properly perfused. Based on this, many workers judge that perfusion is being performed accurately, and it is judged that systemic perfusion has not been achieved unless this event occurs. As a result of examining the frequency of blood removal from the liver, as shown in Table 1, when the existing needle was used, an event in which the liver was not removed was observed in 2 out of 5 cases. On the other hand, with the injection needle device according to this example, good and rapid hepatic blood removal was confirmed in all cases. Therefore, it was found that the success rate of systemic perfusion was also dramatically improved by this proposal.

As a puncture error of the perfusion needle, the perfusate may flow out of the lungs as a result of the perfusate flowing into the pulmonary veins due to the needle tip reaching the left atrium, and leaking from the nasal cavity or the oral cavity through the airway. This means that the perfusate has not accurately reached systemic perfusion, and can be said to be an index indicating failure of perfusion fixation. As shown in Table 1, in this evaluation, perfusion fluid leakage from the nasal cavity and oral cavity was observed in 2 of 5 cases with the needle, and perfusion fixation failed in such cases. On the other hand, in the injection needle device of this example, no leakage of perfusate from the nasal cavity / oral cavity was observed in all cases. It was found that in the injection needle device according to this example, the needle tip was accurately held in the left ventricle from the installation to the end of perfusion, leading to the success of systemic perfusion.

Involuntary muscle contraction occurs when muscle tissue comes into contact with formalin. This is recognized as a characteristic convulsion in individuals, which is an indicator that systemic perfusion of formalin was performed accurately. As a result of investigating the frequency of occurrence of this convulsions, as shown in Table 1, 2 out of 5 cases of existing needles showed failure cases in which convulsions did not occur. On the other hand, convulsions were confirmed in all cases with the injection needle device according to this example. Therefore, it was found that even a beginner can succeed in systemic perfusion without mistake with the proposed needle device.

The most reliable evaluation index for final perfusion fixation is systemic hardening after perfusion fixation. Regarding the hardening of the whole body after the perfusion fixation treatment, no hardening was observed (0), some hardening was observed but not sufficient (1), and sufficient hardening was observed (2). As shown, the evaluation of (2), which can be judged as successful with the existing needle, was recognized in only one case. On the other hand, with the injection needle device of this example, hardening of the whole body (2) was confirmed in all cases.

As a result of examining the success or failure of the final perfusion fixation as a result of integrating the above findings, the case where the perfusion fixation was successful by the beginner's work was 1 in 5 cases with the existing needle, whereas in this example All of the 5 cases were such injection needle devices. In addition, as a result of interviewing about the feeling of use, as shown in Table 1, the injection needle device of this example is high in the difficulty and anxiety about the work with the existing needle and the feeling of use that the work is without oneself. The comment was that it was a feeling of security and stress-free use.

It was found that the injection needle device according to this example has extremely high usefulness that greatly increases the success rate of perfusion experiments and almost no failure even for beginners. Furthermore, by reducing the stress of the user by simple operation and guaranteeing the success rate, and by reducing the number of animals that drop out of the experiment due to the failure of perfusion, the total number of animals used can be reduced. This leads not only to the economic aspect but also to the reduction of animals used as set forth in the ethical guidelines for animal experiments. In addition, from the examples, with the existing needles, although blood was removed, formalin fixation was insufficient, and as a result, there were cases in which the individual was not desirablely cured. At first glance, perfusion fixation seems to be successful, so it is possible that it will be adopted as experimental data even though it was originally a failure. In that case, the data of the tissue in which the formalin is not fixed accurately will be mixed, which will lead to the loss of the reliability and stability of the data. In the example using the injection needle device according to this example, such an example of insufficient fixation is not observed, and accurate data can be obtained in all the individuals. Therefore, according to the present invention, it is possible to stabilize experimental data and improve reliability.

The use of the injection needle device according to this embodiment is not limited to formalin perfusion fixation. A high effect can be expected in the blood removal treatment performed for the purpose of avoiding blood contamination in the tissue to be collected. Further, in the dispersed culture of tissues such as liver, a method of collecting cells by systemically perfusing a proteolytic enzyme solution is known, and it can also be used for such applications.

It can be used for perfusion of laboratory animals.

100: Injection needle 110: Needle tip 200: Cylinder spacer 210: Tip 220: 1st plate 221: 1st adhesive surface 230: 2nd plate 231: 2nd adhesive surface 240: Plate 250: Taniori line 300: Clip 310: Pressure welding part 320: Knob part 330: Pivot part 340: Arm member 350: Elastic part 360: Mounting part 400: Return member 700: Heart 710: Left ventricle 720: Right ventricle 730: Interventricular septum 740: Apical part 750 : Heart base 800: Injection needle support fixture 900: Injection needle device

Claims (7)

An injection needle support fixture that supports and fixes the injection needle when injecting a drug into a tissue.
An injection needle support portion that exposes the needle tip of the injection needle to support the injection needle, and
An injection needle fixing portion for fixing an injection needle punctured by the tissue by being attached to the tissue,
Have a,
The injection needle support portion includes a first plate portion and a second plate portion, and the first plate portion and the second plate portion are detachably adhered to a first adhesive surface and a second adhesive surface, respectively. The injection is characterized in that the needle tip of the injection needle is exposed between the first plate portion and the second plate portion, and the injection needle is adhesively supported while sandwiching the injection needle. Needle support fixture. The injection needle support portion is formed by having the first plate portion and the second plate portion formed of a single plate portion and folding the injection needle support portion along a valley fold line that separates the first plate portion and the second plate portion. The injection needle support fixture according to claim 1 , wherein the needle tip of the injection needle is exposed between the first plate portion and the second plate portion to sandwich the injection needle. In the injection needle support portion, the first adhesive surface and the second adhesive surface are each covered with a seal portion, and the first adhesive surface and the second adhesive surface are exposed by peeling the seal portion. The injection needle support fixture according to claim 1 or 2 . The injection according to any one of claims 1 to 3 , wherein the injection needle fixing portion is a clip attached to a tissue, and the clip is provided on a side portion of the injection needle support portion. Needle support fixture. The clip comprises a pair of arm members that grip the tissue.
The injection needle according to claim 4 , wherein one arm member abuts on the front surface side of the tissue, the other arm member abuts on the rear surface side of the tissue, and the clip grips the tissue. Support fixture. The clip
A pair of arm members pivotally supported at approximately intermediate positions, with a pressure contact at the tip and a knob at the rear.
The injection needle support fixture according to claim 4 or 5 , further comprising an elastic portion that grips a tissue by being urged by an elastic force in a direction that brings each pressure contact portion closer to each other. The injection needle support fixture according to claim 6 , wherein a plurality of streaky uneven portions are formed on the surfaces facing each other in the pressure contact portion.