JP6753662B2 - Anti-glycation agent - Google Patents

Description

The present invention relates to an anti-glycation agent.

In recent years, glycation stress on living organisms has attracted attention as a major risk factor for accelerating aging. Glycation stress is the reaction of reducing sugars and aldehydes with proteins and amino acids to produce carbonyl compounds and advanced glycation end products (AGEs), and the accumulation of these substances causes loads and functions on cells and tissues. It is a concept that includes a series of reactions such as inducing functional decline of sex proteins and further inducing inflammation. The glycation reaction, which is the center of glycation stress, is a reaction in which proteins and reducing sugars bind non-enzymatically to produce glycated products. The glycated products include pentocidin, which is AGEs, and 3-deoxyglucosone, which is a production intermediate. Substances such as 3-deoxyglucosone (3DG) have been reported. In vivo accumulation of glycated products is considered to be involved in skin sclerosis, arteriosclerosis, osteoporosis, infertility and the like, and these diseases generally increase with aging. Therefore, the effect of reducing saccharification stress by suppressing the production of glycated products and removing decomposition is attracting attention as a new proposal for anti-aging. Especially in women, the risk of these diseases increases rapidly with aging, so it is considered that reduction of glycation stress leads to improvement of women's QOL (Non-Patent Document 1).

On the other hand, peony root has long been used as a medicine such as Chinese herbal medicine. Further, it is known that Shakuyaku has a deodorizing action (Patent Document 1), a mucopolysaccharide fragmentation suppressing action, an active oxygen scavenging action (Patent Document 2), and a melanin production suppressing action (Patent Document 3). However, it has not been reported so far that peony plants such as peony have an anti-glycation effect. Furthermore, it has not been reported that there is a correlation between the deodorant effect, the mucopolysaccharide fragmentation inhibitory effect, the active oxygen scavenging effect, the melanin production inhibitory effect, and the anti-glycation effect.

Japanese Patent No. 4868323 Japanese Patent No. 3532244 Japanese Patent No. 4659378

Food STYLE 21, Vol.19, No.9, 2015

An object of the present invention is to provide a novel anti-glycation agent.

As a result of intensive research to solve the above problems, the present inventors have found that the flower part of a Peony plant or a processed product thereof has an anti-glycation effect, and further research is carried out based on this finding. The present invention has been completed.

That is, the present invention relates to the following invention.
[1] An anti-glycation agent containing a flower part of a Peony plant or a processed product thereof.
[2] The agent according to the above [1], wherein the peony plant is peony.
[3] The agent according to the above [1] or [2], which is a drug, a quasi drug, a cosmetic product, a miscellaneous product, a food or drink, a food additive or a feed.
[4] The agent according to any one of the above [1] to [3], which is an anti-aging agent.
[5] Any one of the above [1] to [4] for preventing and / or treating skin sclerosis, arteriosclerosis, diabetes, osteoporosis, infertility, hair loss, Alzheimer's disease type dementia, cataract or sexual dysfunction. The agent described in the section.
[6] Supplements, dietary supplements, health foods or special-purpose foods containing the agent according to any one of the above [1] to [5], which is indicated to have the concept of anti-glycation.
[7] The agent according to any one of the above [1] to [5] or the food according to the above [6], which is used for reducing glycation stress or anti-aging.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide an anti-glycation agent containing a flower part of a Peony plant or a processed product thereof. Since the flower part of the button family plant or its processed product is a safe product derived from natural products, it is possible to take daily medicines, quasi-drugs, cosmetics, miscellaneous goods, foods and drinks (supplements, dietary supplements, health). It can be widely used as functional foods, special purpose foods, etc.), food additives, feeds, etc. According to the present invention, effective use of waste becomes possible. The flower part of the Peony plant is also excellent in terms of ease of collection. In the present invention, the extraction of the active ingredient or its content can be carried out in an industrially advantageous manner. The present invention is also excellent in that an anti-glycation agent can be produced at low cost. The present invention is also excellent in that the anti-glycation effect per unit amount of the anti-glycation agent is large.

It is a figure which shows the AGEs production suppression rate of the peony petal extract.

The present invention provides an anti-glycation agent containing the flower part of a Peony plant or a processed product thereof as an active ingredient. The anti-glycation agent of the present invention may be any as long as it contains a flower part of a Peony plant or a processed product thereof. Paeoniaceae plants in the present invention include, for example, Paeonia japonica (Makino) Miyabe et Takeda, Paeonia japonica (Makino) Miyabe et Takeda f. Hirsuta H. Hara, and Paeonia obovata. lactiflora Pall. Var. lactiflora), Paeonia lactiflora Pall. Var. Trichocarpa (Bunge) Stearn), Paeonia obovata Maxim., Paeonia obovata Maxim. F. albiflora M. .Shimizu), Paeonia obovata Maxim. F. glabra (Makino) Kitam., Paeonia obovata Maxim. F. Oreogeton (S. Moore) Kitag.), Paeonia officinalis L. ), Button (Paeonia suffruticosa Andrews), Can button (Paeonia suffruticosa Andrews'Hiberniflora'), Paeonia obovata (Paeonia tenuifolia L.) and the like. As peony plants in the present invention, for example, peony varieties such as Shonan, Tani no Homare, Yuzuki, Juliet, Snow White, Kaguya Hime, Kita Chancellor, Beni Nichirin, Yoimachi, Kasuga, Tama Mizuun, Benishizuka, etc. (Paeonia lactiflora Pall.) Or a button (Paeonia suffruticosa Andrews) whose cultivar name is yellow crown, peony, peony, peony, zipang, etc. may be used. The part of the Peony plant used is the flower part. Furthermore, for example, underground parts such as roots; above-ground parts such as stems, seeds, and leaves; combinations of two or more of these; etc. may be used in combination, and applicable laws should be observed in actual use. Deaf, but not particularly limited.

As the flower part of the Peony family plant in the present invention, for example, the flower part of the Peony family plant may be used as it is, or a dried, shredded, or crushed flower part may be used. As the processed product of the Peony plant in the present invention, for example, a processed product obtained by extracting the flower part of the plant of the Peony family with an extraction solvent may be used. The above-mentioned processed product can usually take the form of an extract with an extraction solvent, a diluted solution thereof, a concentrated solution thereof, or a dried product from which the extraction solvent has been removed. Specifically, the processed product may take, for example, a solution, a paste, a gel, a powder, or the like.

Examples of the extraction solvent include water; aliphatic monohydric alcohols such as methanol, ethanol and propanol; aliphatic polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol; ketones such as acetone; diethyl ether and dioxane. Ethers such as, nitriles such as acetonitrile; esters such as ethyl acetate ester; aromatics such as xylene, benzene and toluene; organic solvents such as alkyl halides such as chloroform.
These extraction solvents may be used alone or in admixture of two or more. The mixing ratio of the extracted extraction solvent to be mixed is not particularly limited, and is appropriately adjusted.

As the extraction solvent, an extraction solvent containing an aliphatic alcohol such as an aliphatic monohydric alcohol or an aliphatic polyhydric alcohol is preferable, and an aliphatic 1 is preferable, because the anti-glycation agent becomes more excellent in anti-glycation performance. An extraction solvent containing a valent alcohol is more preferable, an extraction solvent containing ethanol is further preferable, and a mixed solution of ethanol and water is most preferable.

In addition, the amount of extraction solvent is usually 2 to 100 times the amount of the extraction raw material (mass ratio), and the extraction time is 1 hour to 5 days (room temperature extraction) and 10 minutes to 5 hours (heat extraction) at room temperature or under heating. ). The extraction step may be repeated a plurality of times. After the extraction, if necessary, purification treatment such as filtration, deodorization, and decolorization, dilution, concentration, drying, and the like can be performed.

Since the anti-glycation agent of the present invention containing the flower part of the Alzheimer's plant thus obtained or a processed product thereof has an anti-glycation effect, all the symptoms caused by glycation, for example, aging and skin hardening , Arteriosclerosis, diabetes, osteoporosis, infertility, hair loss, Alzheimer's disease, senile blemishes in the brain, cataracts, sexual dysfunction, etc. can be suitably used for prevention and / or treatment. "Treatment" includes concepts such as "mitigation" and "improvement". Since the anti-glycation agent of the present invention has an anti-aging effect, it is useful as an anti-aging agent. The anti-aging agent can suppress the occurrence of wrinkles, age spots, dullness, etc. of the skin, and can suppress the progress after the occurrence.

The content of the flower part of the Peony plant or its processed product in the anti-glycation agent (preparation) of the present invention is not particularly limited, but the flower part of the Peony plant or its processed product is about 0 with respect to the preparation. It is preferably contained from 001 to about 100% by mass, more preferably from about 0.01 to about 100% by mass. The mass ratio (processed product of the flower part of the Peony plant): (the flower part of the Peony plant) is preferably (1:25) to (25: 1), and is preferably (1: 5) to (5:). 1) is more preferable.
The daily dose of the flower part of a Peony plant or a processed product thereof varies depending on the administration subject, but for example, when the subject is an adult human, it is usually about 1 to about 17,000 mg / day, preferably about 10 to about. It is 5000 mg / day.

The anti-glycation agent of the present invention can be administered to mammals by either oral or parenteral routes. Oral preparations include tablets, capsules, granules, pills, powders, oral tablets, oral solutions, emulsions, suspensions, syrups, jellies, candy-like agents, gummy-like agents, paste-like agents, etc. Can be mentioned. Further, it may be packaged in a tea bag shape and leached in water or hot water before drinking, or it may be dissolved in water or hot water and drunk, or added to food or drink and ingested. Parenteral preparations include injections (eg, subcutaneous injections, intravenous injections, intramuscular injections, intraperitoneal injections), instillations, external skin preparations (eg, nasal preparations, transdermal preparations, ointments). Agents, lotions, emulsions, gels, creams), eye drops, ear drops, nasal drops, suppositories (eg, rectal suppositories, vaginal suppositories), packs, baths, soaps (including wash pigments) ) Etc. can be mentioned. These formulations can be formulated with pharmaceutically acceptable additives by methods commonly practiced in the art. Pharmaceutically acceptable additives include stabilizers, surfactants, lubricants, buffers, sweeteners, flavoring agents, binders, antioxidants, coating agents, flavoring agents / fragrances, and sugar coatings. Agents, tonicity agents, emulsifiers, thickening agents, pH adjusters, excipients, dispersants, disintegrants, preservatives, preservatives, solubilizers, etc., such as magnesium carbonate, stearer. Magnesium carbonate, talc, sugar, lactose, pectin, dextrin, starch, gelatin, tragant, methyl cellulose, sodium carboxymethyl cellulose, low melting point wax, cacao butter and the like can be used as excipients or carriers.

When the antisaccharide agent of the present invention is an oral preparation, it is preferably a solid preparation (tablets, capsules, granules, rounds, powders, oral tablets), and the active ingredient is, for example, an excipient (lactose, lactose, Sugars such as sucrose and dextrin, starches such as corn starch and pregelatinized starch, celluloses such as crystalline cellulose and cellulose, sugar alcohols such as mannitol, martitol and erythritol, silicic acid, silicon dioxide, and aluminic acid metasilicate. Silicon compounds such as magnesium, calcium compounds such as calcium carbonate and calcium phosphate, aluminum compounds such as aluminum oxide and aluminum hydroxide, magnesium compounds such as magnesium carbonate and magnesium oxide or minerals such as starch and titanium oxide), binders (methacrylic acid) Acrylic acid polymers such as copolymers, celluloses such as hypromerose and hydroxypropyl cellulose, starches such as epoxy resins, corn starch and pregelatinized starch, vinyl polymers such as polyvinyl alcohol and carboxyvinyl polymer, sugars such as pectin and purulan. ), Disintegrants (surfactants such as sodium lauryl sulfate, macrogol, polysorbate, celluloses such as crystalline cellulose, hydroxypropyl cellulose, croscarmellose sodium, starches such as hydroxypropyl starch, corn starch, pregelatinized starch, etc. Povidones such as povidone and crospovidone, sugars such as agar and mannitol), lubricants (surfactants such as sodium lauryl sulfate, macrogol and polysorbate, and silicon compounds such as silicic acid, silicon dioxide and magnesium aluminometasilicate). , Starch acid and its salts, fats and oils such as beeswax and carnauba wax) and the like, and can be formulated according to a conventional method. If necessary, sugar coating (if necessary, a film is formed using acrylic acid polymer, cellulose, epoxy resin, vinyl resin, shelac, etc. in the formulation, and sugars, sugar alcohols, aluminum compounds, calcium compounds, silicon compounds, etc. are formed. , Minerals, resins, gelatins, celluloses, starches, magnesium compounds, oils and fats, etc.), film coating (acrylic acid polymer, celluloses, epoxy resin, vinyl resin, shelac, etc.) Add sugars, sugar alcohols, aluminum compounds, calcium compounds, silicon compounds, minerals, resins, surfactants, gelatins, celluloses, starches, magnesium compounds, fats and oils, etc. to the film as needed. Can be) or the like. Further, it may be a multi-layer lock or a nucleated lock.

Oral solutions (oral solutions, elixirs, suspensions, emulsions, limonades, syrups, etc.) dissolve and suspend the active ingredient in commonly used diluents (water, ethanol, or a mixture thereof). It becomes turbid or emulsified and is formulated. Furthermore, this liquid agent is a stabilizer, a surfactant, a buffer, a sweetener, a flavoring agent, an antioxidant, a flavoring agent / fragrance, an isotonic agent, an emulsifier, a viscous agent, and a pH adjuster. It may contain agents, dispersants, preservatives, preservatives, solubilizers and the like.

Examples of parenteral preparations include external preparations for skin. The external preparation for skin can be applied to solid preparations, liquid preparations, sprays, ointments, creams, gels, patches and the like, but is not limited to these as long as it is a dosage form suitable for external use. Topical skin preparations are commonly used as needed to obtain water, lower alcohols, vasodilators, surfactants, emulsion stabilizers, gelling agents, adhesives, and other desired dosage forms. The base component can be blended, and vasodilators, corticosteroids, moisturizers, bactericides, refreshing agents, vitamins, fragrances, pigments, etc. can be blended appropriately as long as the effects of the present invention are not impaired. It is also possible to do.

Parenteral preparations include, for example, injections. Injections include solutions, suspensions, emulsions, and solid injections that are used by dissolving or suspending in a solvent at the time of use. Injectables are formulated by dissolving, suspending or emulsifying the active ingredient in a solvent. As the solvent, for example, distilled water for injection, physiological saline, vegetable oil, propylene glycol, polyethylene glycol, alcohols such as ethanol, and the like, and combinations thereof are used. Further, this injection may contain a stabilizer, a solubilizing agent (glutamic acid, aspartic acid, polysorbate 80 (registered trademark), etc.), a suspending agent, an emulsifier, a soothing agent, a buffering agent, a preservative, and the like. .. These are sterilized in the final step or manufactured by aseptic technique. It is also possible to produce a sterile solid preparation, for example, a freeze-dried product, and dissolve it in sterile or sterile distilled water for injection or other solvent before use.

The oral preparation or parenteral preparation of the present invention includes pharmaceuticals, quasi-drugs, cosmetics, miscellaneous goods, foods and drinks (including supplements, dietary supplements, health foods, special purpose foods, etc.), food additives, feeds, etc. It can be carried out in an embodiment. In the present invention, the miscellaneous goods refer to products that are not subject to the Act on Securing Quality, Effectiveness, and Safety of Pharmaceuticals, Medical Devices, etc., and examples thereof include sprays and creams.

When the anti-glycation agent of the present invention is a pharmaceutical embodiment, the flower part of the Peony plant of the present invention or a processed product thereof is used as an active ingredient, and a pharmaceutically acceptable carrier or additive is appropriately blended and formulated. can do. The dosage form is not particularly limited, and preferably, for example, oral preparations such as tablets, capsules, granules, suppositories, powders, oral tablets, oral solutions, syrups, emulsions, suspensions, jellies; (For example, subcutaneous injection, intravenous injection, intramuscular injection, intraperitoneal injection), infusion, drip, external skin preparation (eg, nasal preparation, transdermal preparation, ointment), eye drops, It can be a parenteral preparation such as ear drops, nasal drops, suppositories (for example, rectal suppositories, vaginal suppositories), ointments, patches, liquids and the like. It is preferably an external preparation.

The blending ratio of the additive used for the formulation of the pharmaceutical product of the present invention may be appropriately set based on the range usually adopted in the pharmaceutical field. The additives that can be blended are not particularly limited, but for example, various solvents such as water, physiological saline, other aqueous solvents, aqueous or oily bases; stabilizers, surfactants, lubricants, buffers, etc. Sweeteners, flavoring agents, binders, antioxidants, coating agents, flavoring agents, sugar coating agents, tonicity agents, emulsifiers, thickening agents, pH regulators, excipients, dispersants, disintegrants , Preservatives, preservatives, solubilizers and other various additives.

When the dispensing unit form is a capsule, the above-mentioned type of material can further contain a liquid carrier such as fat or oil as a useful additive. Aseptic compositions for injection can be prepared according to routine formulation practices (eg, dissolving or suspending the active ingredient in a solvent such as water for injection, natural vegetable oil, etc.). As the aqueous solution for injection, for example, a physiological saline solution, an isotonic solution containing glucose and other auxiliary agents (for example, an aqueous solution containing D-sorbitol, D-mannitol, sodium chloride, etc.) is used and is suitable. Sodium solubilizers such as alcohol (eg ethanol), polyalcohol (eg propylene glycol, polyethylene glycol), nonionic surfactants (eg Polysorbate 80 ^TM , HCO-50) and the like may be used in combination. .. As the oily liquid, for example, sesame oil, soybean oil and the like are used, and may be used in combination with benzyl benzoate, benzyl alcohol and the like as solubilizing agents. Also, buffers (eg, phosphate buffer, sodium acetate buffer), soothing agents (eg, benzalkonium chloride, prokine hydrochloride, etc.), stabilizers (eg, human serum albumin, polyethylene glycol, etc.), storage. It may be blended with an agent (for example, benzyl alcohol, phenol, etc.), an antioxidant, or the like.

When the anti-glycation agent of the present invention is in the form of a quasi-drug, cosmetic or miscellaneous product, the form is not particularly limited. Preferably, for example, skin external preparations (including lotions, emulsions, foundations, hand creams, beauty essences, etc.), shampoos, conditioners, treatments, hair care agents, styling agents, packs, soaps (including facial cleansers), body shampoos, etc. It can be in the form of a hair restorer or a bathing agent.

When the antisaccharifying agent of the present invention is a non-pharmaceutical product, cosmetic or miscellaneous product, an ingredient generally used as a non-pharmaceutical product, cosmetic or miscellaneous product other than the flower part of the button family plant of the present invention or a processed product thereof, for example. , Stabilizers, surfactants, lubricants, buffers, sweeteners, flavoring agents, binders, antioxidants, coating agents, wetting agents, flavoring agents / fragrances, coloring agents, sugar coating agents, etc. Tensioning agents, emulsifiers, thickening agents, pH adjusters, excipients, dispersants, disintegrants, preservatives, preservatives, solubilizers, solubilizers, oils, moisturizers, UV absorbers, filling For the purpose of agents, metal ion sequestering agents, sunscreen agents, defoaming agents, softeners, propellants, acidifying or basicizing agents, silicones, vitamins, dyes, pigments, nanopigments, organic solvents such as alcohol, water, etc. Can be appropriately blended according to the above.

Preferred dosage forms of quasi-drugs, cosmetics or miscellaneous products include dosage forms such as solids, liquids, lotions, emulsions, gels, creams, ointments and aerosols, whichever are suitable for external use. However, it is not limited to these.
Topical skin preparations are commonly used as needed to obtain water, lower alcohols, vasodilators, surfactants, emulsion stabilizers, gelling agents, adhesives, and other desired dosage forms. The base component can be blended, and vasodilators, corticosteroids, moisturizers, bactericides, refreshing agents, vitamins, fragrances, pigments, etc. can be blended appropriately as long as the effects of the present invention are not impaired. It is also possible to do.

The preparation thus obtained can be administered to, for example, humans and other animals (eg, rats, mice, rabbits, sheep, pigs, cows, cats, dogs, monkeys, etc.).

The present invention is not the food or drink itself, but the food or drink (supplement, nutritional supplement, health functional food, special use) to which the flower part of a peony plant for promoting anti-glycation or a processed product thereof is added or increased. (Including food etc.) is also provided. The food or drink of the present invention may contain the above-mentioned anti-glycation agent of the present invention. The form of the food or drink is not particularly limited, but may be, for example, a processed form such as a naturally liquid diet, a semi-digested nutritional diet or an elemental diet, or a drink. Foods and drinks include, for example, tea drinks, soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks and other beverages, soba, udon, Chinese noodles, instant noodles and other noodles, candy, candy, gum, chocolate and snacks. , Biscuits, jelly, jam, cream, baked goods, bread and other sweets and breads, fishery and livestock processed foods such as kamaboko, ham and sausage, processed milk, fermented milk and other dairy products, salad oil, tempura oil, margarine, Oils and fats such as mayonnaise, shortening, whipped cream, dressing, processed oil and fat foods, seasonings such as sauces and sauces, retort pouch foods such as curry, stew, bowls, porridge, miscellaneous dishes, ice cream, sherbet, chilled confectionery such as shaved ice, etc. Can be mentioned. In addition, the food and drink of the present invention includes supplements, dietary supplements, health functional foods, special-purpose foods, etc., which are indicated to have the concept of anti-glycation. In addition, the food and drink of the present invention may contain pharmaceutical excipients such as lactose, starch, crystalline cellulose, and sodium phosphate.

The present invention provides a food additive for promoting anti-glycation, not the food or drink itself. The food additive of the present invention may contain the above-mentioned anti-glycation agent of the present invention. The form of the food additive of the present invention is not particularly limited, and examples thereof include liquid, paste, powder, flakes, and granules. The food additives of the present invention also include additives for beverages. The food additive of the present invention can be produced according to a general method for producing a food additive.

The present invention provides a feed for promoting anti-glycation. The feed of the present invention may contain the above-mentioned anti-glycation agent of the present invention. Examples of the feed include livestock feeds such as cattle, horses, pigs, sheep, goats and rabbits, and pet feeds such as dogs and cats. The feed of the present invention can be processed and manufactured by using a general feed manufacturing method other than adding the anti-glycation agent of the present invention or the like to the feed.

The fact that the present invention has an anti-glycation effect is that the ability to suppress AGEs production is measured, for example, according to the method of Lee et al. (Lee, EH et al .: Biol. Pharm. Bull., 31, 1626-1630 (2008)). Can be confirmed by. The anti-glycation agent of the present invention may also have an antioxidant effect in addition to the anti-glycation effect. In general, no correlation has been confirmed between the antioxidant effect and the anti-glycation effect. In addition, the flower part of a Peony plant or a processed product thereof, which is the active ingredient of the anti-glycation agent of the present invention, can be used additively or synergistically by using it in combination with other active ingredients used for anti-glycation. Effect can be expected to improve. Other active ingredients used for anti-glycation include legumes, matatabiaceae, pomegranates, spiders, spiders, cereals, roses, maple plants, hirugaos. , Abrana family, Rice plant, Kiku family plant, Dokudami family plant, Vine family plant, Obako family plant, Tochinoki family plant, Fukugi family plant, Mokusei family plant, Tsubaki family plant, Mikan family plant, Futomomo family plant, Kakinoki Examples thereof include family plants, Akane family plants, Beech family plants, Hydrangea family plants, Hishi family plants, Misohagi family plants, and Gagaimo family plants. In that a synergistic effect of anti-glycation effect can be obtained, the flower part of a button family plant such as a flower extract of shakyaku or a processed product thereof and a legume family plant, a matatabi family plant, a pomegranate family plant, a Kusunoki family plant, a Todaigusa family plant , Seri family plant, rose family plant, maple family plant, hirugao family plant, abrana family plant, rice family plant, kiku family plant, dokudami family plant, grape family plant, obaco family plant, tochinoki family plant, fukugi family plant, mokusei Can be used in combination with family plants, camellia plants, citrus plants, futomomo plants, oyster plants, madder plants, beech plants, hydrangeas plants, hisashi plants, misohagi plants, gagaimo plants, etc. preferable.
The flower part of a Peony plant or a processed product thereof, which is the active ingredient of the anti-glycation agent of the present invention, can be ingested or used for a long period of time.

The present invention includes an anti-glycation method for administering an effective amount of a flower part of a Peony plant or a processed product thereof to a human in need of anti-glycation. The present invention also includes a non-therapeutic anti-glycation method in which the flower part of a Peony plant or a processed product thereof is orally ingested by a person in need of anti-glycation. The term "non-therapeutic" is a concept that does not include medical treatment, that is, treatment of the human body or animal body by treatment.

The present invention includes a method for preventing and / or reducing glycation, which comprises administering an effective amount of the anti-glycation agent of the present invention to an animal in which it is necessary to promote anti-glycation.
The present invention includes the use of the anti-glycation agent of the present invention for anti-glycation.
The present invention includes the anti-glycation agent of the present invention used for anti-glycation.

Hereinafter, the present invention will be described in detail with reference to Examples, but the present invention is not limited thereto.

[Example 1: Production of peony flower extract]
Coarse peony flowers were produced from the petals of peony (Paeonia lactiflora Pall. Var. Trichocarpa (Bunge) Stearn). Coarse peony flowers were obtained by air-drying the peony flowers for about 1 week and chopping them by hand and with a crusher.
450 g of coarse peony flowers were placed in 3600 mL of a mixed solution of water / ethanol (95) (7: 3 (volume ratio)), and the leaching operation was performed by stirring at room temperature for 2 days. Then, suction filtration (filter paper No. 2) was performed to obtain a residue and a first leachate. The residue was placed in 3600 mL of a water / ethanol (95) mixture (7: 3 (volume ratio)) and allowed to exude with stirring at room temperature for 2 days. Suction filtration (filter paper No. 2) was performed to obtain a second leachate. The first leachate and the second leachate were mixed and concentrated under reduced pressure at 60 ° C. or lower until the consistency for starch syrup was obtained to obtain a peony flower extract.

[Example 2: Test method]
After reacting D-glucose, bovine serum albumin and the test solution at 60 ° C. for 48 hours according to the method of Lee et al. (Lee, EH et al .: Biol. Pharm. Bull., 31, 1626-1630 (2008)). , The AGEs production inhibitory ability measurement test was carried out by measuring the fluorescence intensity of the reaction solution. The ability to suppress AGEs production was evaluated as a relative value when the production rate of the untreated control to which the test solution was not added was set to 100%. Moreover, the final reaction solution an IC ₅₀ value from the graph of test concentrations and inhibition rate in (50% inhibitory concentration) was calculated.
(1) Preparation of reaction solution After extracting 2.0 g of Shakyaku flower extract with 50 mL of 50% (volume ratio) ethanol solution, centrifuge (3000 rpm, 5 minutes), and filter the obtained filtrate to 50% ethanol solution. Diluted appropriately with to prepare a test solution.
100 μL of 100 mg / mL bovine serum albumin (BSA; Bovine Serum Albumin; product number A3733, manufactured by Sigma Aldrich) solution, 500 μL of 200 mg / mL D-glucose solution, 20 μL of test solution and 1/15 mol / L phosphate buffer in a 1.5 mL microtube. After adding 380 μL of the solution (pH 7.2), the reaction solution was heated at 60 ° C. for 48 hours.
(2) Measurement of Fluorescence Intensity 20 μL of the reaction solution was added to a 96-well microplate, 200 μL of water was added, and then the fluorescence intensity was measured with a microplate reader.
The ability to suppress AGEs production was calculated as follows, using a solution prepared by adding 1/15 mol / L phosphate buffer (pH 7.2) instead of the test solution and operating in the same manner as an untreated control. The blank used was prepared by adding 1/15 mol / L phosphate buffer (pH 7.2) instead of the D-glucose solution and operating in the same manner.

Microplate reader operating conditions Model: SpectraMax M2e
Measurement conditions: fluorescence, endpoint mode, bottom read excitation wavelength: 370 nm
Fluorescence wavelength: 440 nm

The results are shown in Table 1 and FIG.

From Table 1 and FIG. 1, it was confirmed that the peony petal extract suppresses the production of AGEs in a concentration-dependent manner. Incidentally, _{IC 50} values calculated from the graph showing the relationship between sample concentration and production inhibition rate was 0.21 mg / mL.

The present invention is not limited to the above-described embodiments and examples, and various modifications can be made within the scope of the claims, and the technical means disclosed in the different embodiments may be appropriately combined. The obtained embodiments are also included in the technical scope of the present invention.

Claims (6)

An anti-glycation agent containing 0.01 to 100% by mass of an ethanol extract of a peony flower. The agent according to claim 1, which is a pharmaceutical product, a quasi drug, a cosmetic product, a miscellaneous product, a food or drink, a food additive, or a feed. The agent according to claim 1 or 2, which is an anti-aging agent. The agent according to any one of claims 1 to 3, which is for preventing and / or treating skin sclerosis, arteriosclerosis, diabetes, osteoporosis, infertility, hair loss, Alzheimer's disease type dementia, cataract or sexual dysfunction. The agent according to any one of claims 1 to 4, wherein the ethanol extract is a hydrous ethanol extract. An anti-glycation supplement, an anti-glycation dietary supplement, an anti-glycation health functional food, or an anti-glycation supplement containing the agent according to any one of claims 1 to 5, which is indicated to be based on the concept of anti-glycation. Special purpose food.