JP6702967B2 - 二重特異性抗体のための精製プラットフォーム - Google Patents

二重特異性抗体のための精製プラットフォーム Download PDF

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JP6702967B2
JP6702967B2 JP2017525320A JP2017525320A JP6702967B2 JP 6702967 B2 JP6702967 B2 JP 6702967B2 JP 2017525320 A JP2017525320 A JP 2017525320A JP 2017525320 A JP2017525320 A JP 2017525320A JP 6702967 B2 JP6702967 B2 JP 6702967B2
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protein
buffer
affinity matrix
affinity
heterodimer
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JP2017524740A5 (https=
JP2017524740A (ja
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アンドリュー タスティアン
アンドリュー タスティアン
クリスティーヌ エンディコット
クリスティーヌ エンディコット
ベンジャミン アダムス
ベンジャミン アダムス
ジョン マッティラ
ジョン マッティラ
ハンネ バク
ハンネ バク
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3819—Affinity chromatography of the nucleic acid-nucleic acid binding protein type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/08—Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1271—Micrococcaceae (F); Staphylococcaceae (F), e.g. Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2017525320A 2014-07-26 2015-07-24 二重特異性抗体のための精製プラットフォーム Active JP6702967B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462029463P 2014-07-26 2014-07-26
US62/029,463 2014-07-26
PCT/US2015/041936 WO2016018740A2 (en) 2014-07-26 2015-07-24 Purification platform for bispecific antibodies

Publications (3)

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JP2017524740A JP2017524740A (ja) 2017-08-31
JP2017524740A5 JP2017524740A5 (https=) 2020-01-30
JP6702967B2 true JP6702967B2 (ja) 2020-06-03

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US (1) US10626142B2 (https=)
EP (2) EP3912987B9 (https=)
JP (1) JP6702967B2 (https=)
KR (1) KR102440737B1 (https=)
CN (1) CN107074906B (https=)
AR (1) AR101262A1 (https=)
AU (1) AU2015298156B2 (https=)
DK (2) DK3912987T3 (https=)
EA (1) EA036154B1 (https=)
ES (2) ES2942533T3 (https=)
FI (1) FI3912987T3 (https=)
IL (1) IL255198B (https=)
MX (1) MX377558B (https=)
MY (1) MY187051A (https=)
PL (2) PL3912987T3 (https=)
SG (2) SG11201700157VA (https=)
TW (1) TWI704155B (https=)
WO (1) WO2016018740A2 (https=)
ZA (1) ZA201700622B (https=)

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EP3172221B1 (en) 2021-07-07
EA036154B1 (ru) 2020-10-06
WO2016018740A3 (en) 2016-03-17
BR112017001443A2 (pt) 2017-12-05
SG10201900661YA (en) 2019-02-27
AR101262A1 (es) 2016-12-07
EP3172221A2 (en) 2017-05-31
WO2016018740A2 (en) 2016-02-04
MX2017001217A (es) 2017-05-03
CN107074906A (zh) 2017-08-18
MY187051A (en) 2021-08-27
SG11201700157VA (en) 2017-02-27
PL3912987T3 (pl) 2023-07-24
EA201790247A1 (ru) 2017-08-31
FI3912987T3 (fi) 2023-06-19
US20160024147A1 (en) 2016-01-28
IL255198B (en) 2022-04-01
EP3912987B9 (en) 2023-05-31
IL255198A0 (en) 2017-12-31
MX377558B (es) 2025-03-10
DK3912987T3 (da) 2023-05-01
DK3172221T3 (da) 2021-08-09
KR102440737B1 (ko) 2022-09-06
ES2881026T3 (es) 2021-11-26
EP3912987A1 (en) 2021-11-24
ZA201700622B (en) 2020-05-27
WO2016018740A8 (en) 2021-06-10
CN107074906B (zh) 2021-05-14
US10626142B2 (en) 2020-04-21
KR20170035941A (ko) 2017-03-31
ES2942533T3 (es) 2023-06-02
EP3912987B1 (en) 2023-03-22
AU2015298156A1 (en) 2017-02-23
JP2017524740A (ja) 2017-08-31
PL3172221T3 (pl) 2021-12-27
TWI704155B (zh) 2020-09-11
CA2955618A1 (en) 2016-02-04
TW201617359A (zh) 2016-05-16
NZ728700A (en) 2023-09-29
AU2015298156B2 (en) 2020-07-16

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