JP6669829B2 - Fermented tea - Google Patents

Description

  The present invention relates to a fermented tea product. More specifically, the present invention relates to various compositions containing a catechin metabolite, which is a fermented tea product, a method for producing the composition, a method for producing food and drink using the composition obtained by the production method, and the food and drink.

  Awabancha produced in Tokushima Prefecture, Goishi tea produced in Takachi Prefecture, and other teas fermented with microorganisms are known to have various effects and effects, and some reports have shown higher effects than green tea. (Non-Patent Documents 1 and 2). Non-Patent Document 1 reports that Awabancha has a higher energy metabolism-enhancing effect than green tea. It is thought that when energy metabolism is enhanced, body temperature rises, which in turn leads to various health effects such as improvement of chilling, anti-obesity, and increased metabolism, but little is known about its effective components in microbial fermented tea. On the other hand, in Non-Patent Document 3, 1- (3 ′, 4 ′, 5′-trihydroxyphenyl) -3- (2 ″, 4 ″, 6 ″-) in which epigallocatechin is reductively opened in microbial fermented tea It reports that it contains trihydroxyphenyl) -propan-2-ol. To date, 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol has been produced by certain intestinal bacteria However, there is no report on its biological activity.

YAKUGAKU ZASSHI 128 (7) 1037-1044 (2008) The Journal of Medical Investigation vol.56, p42-48 (2009) Jpn. J. Food Chem. Safety, Vol. 18 (1) (2011) J. Agric. Food Chem. 58, 1313-1321 (2010)

  An object of the present invention is to contain 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient. An object of the present invention is to provide a composition for enhancing energy metabolism, a composition for enhancing sympathetic nervous activity, a composition for promoting fat burning, a composition for anti-obesity, and a composition for increasing body temperature.

  Still another object of the present invention is to provide a method for producing a composition containing the active ingredient, food and drink using the composition obtained by the method, and a method for producing the same.

The present invention relates to the following [1] to [8].
[1] 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol for enhancing energy metabolism Composition.
[2] Sympathetic nerve activity enhancement containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol Composition.
[3] 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol for promoting fat burning Composition.
[4] An anti-obesity composition comprising 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 ", 4", 6 "-trihydroxyphenyl) -propan-2-ol object.
[5] Composition for increasing body temperature comprising 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 ", 4", 6 "-trihydroxyphenyl) -propan-2-ol object.
[6] 1- (3 ′, 4 ′, 5′-trihydroxyphenyl) -3- (2 ″, 4 ′) comprising a step of fermenting a tea extract with a bacterium belonging to Lactobacillus pentosus. A method for producing a composition containing ', 6''-trihydroxyphenyl) -propan-2-ol.
[7] A method for producing food or drink, comprising using the composition obtained by the production method according to [6] as a raw material for food or drink.
[8] A food or drink comprising the composition obtained by the production method according to [6].

  The composition for enhancing energy metabolism of the present invention has an excellent effect of being excellent in the action of enhancing energy metabolism. The composition for enhancing sympathetic nervous activity of the present invention has an excellent effect of being excellent in enhancing the sympathetic nervous activity. The composition for promoting fat burning of the present invention has an excellent effect of being excellent in promoting action of fat burning. The antiobesity composition of the present invention has an excellent effect of having an excellent antiobesity effect. The composition for increasing body temperature of the present invention has an excellent effect of being excellent in the action of increasing body temperature.

  The composition obtained by the production method of the present invention is 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2 -Concentration of -ol is high, so it is excellent in promoting energy metabolism, promoting sympathetic nerve activity, promoting fat burning, anti-obesity, and increasing body temperature. It has the effect.

Figure 1 shows the formation of 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol in the fermented broth of tea extract (A: purified fermented catechin product, B: tea extract before fermentation, C: tea extract after fermentation (after culturing for 72 hours using TUA4337L)). Figure 2 shows the time course of 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol in the fermented broth of tea extract. It is a figure which shows a typical production amount. Figure 3 shows the results of the production of 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol by various lactic acid bacteria. FIG. FIG. 4 shows the results of enhancing sympathetic nervous activity by 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol. FIG. FIG. 5 shows day 4 of continuous administration of 1- (3 ′, 4 ′, 5′-trihydroxyphenyl) -3- (2 ″, 4 ″, 6 ″ -trihydroxyphenyl) -propan-2-ol. (*: P <0.05 t-test). FIG. 6 shows the change in respiratory quotient by 1- (3 ′, 4 ′, 5′-trihydroxyphenyl) -3- (2 ″, 4 ″, 6 ″ -trihydroxyphenyl) -propan-2-ol. (*: P <0.05 t-test). FIG. 7 is a diagram showing the results of an increase in body temperature by 1- (3 ′, 4 ′, 5′-trihydroxyphenyl) -3- (2 ″, 4 ″, 6 ″ -trihydroxyphenyl) -propan-2-ol. (*: P <0.05 t-test). FIG. 8 shows the results of suppression of weight gain by 1- (3 ′, 4 ′, 5′-trihydroxyphenyl) -3- (2 ″, 4 ″, 6 ″ -trihydroxyphenyl) -propan-2-ol. It is a figure (†: p <0.1 t-test).

  The composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature of the present invention is 1- (3 ′, 4 ′, 5 ′). -trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient. That is, the composition of the present invention, respectively, 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol It is also an energy metabolism enhancer, a sympathetic nerve activity enhancer, a fat burning enhancer, an anti-obesity agent, or a body temperature increasing agent as an active ingredient. As used herein, `` energy metabolism '' means a process of converting energy released when ATP is converted to ADP into muscle contraction, nerve transmission, substance composition, body temperature regulation, etc. Calculated as energy consumption from carbon dioxide emissions. “Sympathetic nerve” means a sympathetic nerve in brown adipose tissue, which is a heat-producing tissue. When sympathetic nerve activity is increased, energy metabolism is increased, leading to an increase in body temperature, fat burning, and eventually anti-obesity. "Fat burning" uses a respiratory quotient, which is a volume ratio of carbon dioxide emission to oxygen consumption, as an index, and means that the lower the respiratory quotient, the more fat is burned. "Anti-obesity" means that weight gain is suppressed or weight loss occurs.

  1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 ", 4", 6 "-trihydroxyphenyl) -propan-2-ol has the following formula (I):

And is known as a metabolite of epigallocatechin, a kind of catechin.

  1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol can be prepared according to a known organic chemical synthesis method. For example, it can be prepared by hydrogenation using epigallocatechin as a material and a platinum group catalyst. 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol is produced from epigallocatechin by biological transformation Is known (Non-Patent Document 4). In the present invention, it can be obtained, for example, by culturing bacteria belonging to Lactobacillus pentosus using tea extract.

  The bacterium belonging to Lactobacillus pentosus is not particularly limited as long as it is a known bacterium. For example, as an identification number NRIC 0883, accession number NITE BP-1479, and on an international administration date of December 10, 2012, an independent administrative Lactobacillus pentosus TUA4337L strain deposited at the Patent and Microorganisms Depositary Center (2-5-8 Kazusa Kamatari, Kisarazu-shi, Chiba, Japan) can be suitably used. Hereinafter, Lactobacillus pentosus TUA4337L is abbreviated as TUA4337L.

  The obtained processed product may be subjected to a process such as filtration, centrifugation, concentration, ultrafiltration, freeze-drying, powderization, or fractionation, if desired, according to a known method. Thus, for example, a composition containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol, The composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature of the present invention can be provided.

  1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol obtained as described above is a brown fat It can be used to stimulate the sympathetic nervous activity of tissues to activate brown adipose tissue, for example, to exert an action of increasing energy metabolism, an action of increasing body temperature, an action of burning fat, and thus an anti-obesity action.

  The administration form of the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature of the present invention is not limited. In addition, in this specification, administration is used as including all aspects of administration, ingestion, taking, and drinking.

  The effects of the administration of the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature of the present invention are expressed by the administration thereof. There is no particular limitation as long as it is effective. For example, increase of body temperature, improvement of coldness, anti-obesity, reduction of visceral fat, beauty, promotion of metabolism, immunostimulation, anti-fatigue, antidepressant and the like are exemplified.

  The composition for enhancing energy metabolism of the present invention, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature are, for example, 1- (3 ′, 4 ′, 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol, if desired, solvent, dispersant, emulsifier, buffer, stabilizer, stabilizer, excipient, Binders, disintegrants, lubricants and the like are added, and in accordance with known methods, tablets, granules, powders, powders, solids such as capsules and the like, usually liquids, suspensions, liquids such as emulsions, etc. It can also be formulated.

  Further, the content of 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol in the above-mentioned form is those of them. Considering the administration form, administration method and the like, the amount is not particularly limited as long as the desired effect of the present invention can be obtained. 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as a content ratio (content) is usually 0.0001 ~ It is about 100% by weight, preferably about 0.001 to 30% by weight, more preferably about 0.01 to 5% by weight. The content of 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol was measured according to a known method. For example, it can be measured by subjecting to HPLC.

  The composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for increasing anti-obesity, or the composition for increasing body temperature of the present invention are prepared by an appropriate administration method according to their form. Is administered. For example, it can be administered internally, externally, and by injection. For internal use, for example, it can be taken orally; for injection, for example, it can be administered intravenously, intramuscularly, subcutaneously, intradermally, etc .; for external use, for example, external preparations such as suppositories May be administered by a suitable administration method.

  The dosage of the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature of the present invention may be in the form, administration method, and use thereof. It is appropriately set depending on the purpose, age, weight, and symptoms of the human or animal to which the composition is to be administered, and is not constant. For example, the effective human intake of 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol in the present invention is It is preferably about 1 mg to 10 g, more preferably about 10 mg to 3 g, and still more preferably about 100 mg to 1 g per day for a human weighing 50 kg. The administration may be performed once or several times a day within a desired dose range. The administration period is also arbitrary.

  In the present specification, the administration target of the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the anti-obesity composition, or the composition for increasing body temperature of the present invention is preferably Humans needing to treat or prevent the onset of energy metabolism, the enhancement of sympathetic nervous activity, the burning of fat, the suppression of weight gain or weight loss, or the effect of increasing body temperature, such as cattle, horses, goats, etc. Pet animals such as domestic animals, dogs, cats and rabbits, or experimental animals such as mice, rats, guinea pigs and monkeys may be used. In addition, as an administration target, not only individuals that require an increase in energy metabolism, but also individuals whose energy metabolism is not low, individuals who need to suppress weight gain, individuals who need to suppress cold, and visceral fat Also included are individuals who need to suppress an increase, individuals who need to suppress a decrease in metabolism, individuals who want to treat or prevent a cold, individuals who need to maintain beauty, and individuals who need to suppress fatigue.

  The present invention also provides an increase in energy metabolism, an increase in sympathetic nervous activity, promotion of fat burning, suppression of weight gain or weight loss, or an individual in need of an increase in body temperature, 1- (3 ', 4', 5 '-trihydroxyphenyl) -3- (2' ', 4' ', 6' '-trihydroxyphenyl) -propan-2-ol or 1- (3', 4 ', 5'-trihydroxyphenyl) -3- (2 ``, 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol comprising administering a therapeutically effective amount of the composition, including a method for enhancing energy metabolism, a method for enhancing sympathetic nervous activity, and fat. Provided are a method for promoting combustion, a method for suppressing or reducing weight gain, and a method for increasing body temperature. An individual who needs to increase energy metabolism, enhance sympathetic nervous activity, promote fat burning, suppress weight gain or decrease body weight, or increase body temperature is defined as the above-described composition for enhancing energy metabolism of the present invention, The same applies to the subject to which the composition for enhancing nerve activity, the composition for promoting fat burning, the composition for anti-obesity, and the composition for increasing body temperature are applied.

  In the present specification, the therapeutically effective amount is 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2- When ol is administered to the individual, the amount of energy metabolism is increased, the amount of sympathetic nervous activity is increased, the amount of fat burning is promoted, the weight gain is suppressed, or the weight gain is suppressed or reduced, as compared with the individual not administered. Volume or body temperature. The specific effective amount is appropriately set depending on the administration form, administration method, purpose of use, age, weight, symptoms, etc. of the individual, and is not constant.

  In the therapeutic method of the present invention, 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan is used so that the therapeutically effective amount is obtained. -2-ol as it is, also contains 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol The composition can be administered. In addition, there is no limitation on the administration method, and for example, it can be administered by oral administration.

  According to the treatment method of the present invention, energy metabolism is enhanced, sympathetic nervous activity is enhanced, fat burning is promoted, anti-obesity (suppresses weight gain or reduces weight), or increases body temperature without causing side effects. It becomes possible.

  Preferred examples of the composition of the present invention include a composition obtained by a production method comprising a step of fermenting a tea extract with a bacterium belonging to Lactobacillus pentosus. In addition, the said manufacturing method may be described as the manufacturing method of this invention.

  Tea leaves contain various catechins, so-called microbial fermented tea leaves naturally fermented in fermentation barrels such as Awabancha and Goishi tea (post-fermented tea leaves) also contain the compound represented by the formula (I). Is known to be. However, microbial species that produce the compound represented by the formula (I) are not known, and are not fermented using a specific microbial species, but are inefficient due to natural fermentation as in the above-mentioned post-fermented tea leaves. The compound represented by the formula (I) is produced together with other compounds. In addition, the post-fermented tea leaves are fermented in the solid state of the raw material (solid fermentation), and finally the fermented solid (tea leaves) becomes a product, so it is necessary to extract the extract separately when drinking it, In addition, the compound represented by the formula (I) may not be extracted properly or may be decomposed. The present inventors surprisingly, by contacting a bacterium belonging to Lactobacillus pentosus with an extract from tea leaves, a composition containing the compound represented by the formula (I) at a high content can be obtained. It has been found for the first time that the composition has an action of enhancing energy metabolism. That is, as one embodiment of the present invention, by fermenting a tea extract with a bacterium belonging to Lactobacillus pentosus, from epigallocatechin, 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 ' The present inventors have found that the metabolism to ', 4' ', 6' '-trihydroxyphenyl) -propan-2-ol is promoted. Fermenting the extract from tea leaves, unlike fermented tea leaves after extracting the extract separately when drinking, the fermented solution can be used as it is, 1- (3 ', 4', 5'-trihydroxyphenyl) -3- ( 2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol can be easily taken. Hereinafter, 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol obtained using tea extract Is described as the fermented catechin of the present invention, and 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 '' obtained using a tea extract The composition containing -trihydroxyphenyl) -propan-2-ol is sometimes referred to as the fermented catechin-containing composition of the present invention.

  As the tea extract used in the present invention, for example, a tea extract obtained by extracting tea leaves with a known solvent can be used. In the present invention, from the viewpoint of producing more fermented catechin of the present invention, an extract containing a large amount of epigallocatechin, which is a precursor of the fermented catechin of the present invention, may be used.

  The “tea leaves” in the present specification include tea leaves such as green tea, black tea, and oolong tea. Examples of the type of tea leaf include leaves of a Camellia plant, for example, leaves of Camellia genus (Camellia sinensis). In the present invention, the tea leaf itself or a crushed product may be used.

  As the solvent, for example, any one of hydrophilic organic solvents such as water, ethyl alcohol, and isopropanol or a combination thereof as appropriate may be used.

  The extraction conditions are not set unconditionally according to the equipment to be used and the amount used. For example, the extraction temperature is 30 to 100 ° C, preferably 40 to 80 ° C. In the present invention, a commercially available tea extract may be used.

  As the bacteria belonging to Lactobacillus pentosus used herein, those described above can be similarly used.

  In addition, examples of the strain in the present invention include various forms such as bacteria (live and dead) belonging to Lactobacillus pentosus, the lactic acid bacteria-containing material, and the processed lactic acid bacteria. Viable bacteria can be obtained from lactic acid bacteria-containing substances such as the lactic acid bacteria culture solution. Dead bacteria can be obtained, for example, by subjecting live bacteria to heating, ultraviolet irradiation, formalin treatment, acid treatment, and the like. The obtained viable and dead bacteria can be further processed by grinding or crushing. The lactic acid bacterium in each of the above embodiments is preferably a viable bacterium as long as it acts on epigallocatechin to produce the fermented catechin of the present invention, but may also contain dead bacterium.

  The fermentation conditions by the fungus are not particularly limited as long as the fermentation is performed in a tea extract, and for example, fermentation by adding additives including a carbon source, a nitrogen source, inorganic salts, and organic nutrients. May be. Specifically, for example, a yeast extract, which is an extract obtained by extracting a component of yeast by treatment with an enzyme, hot water, or the like, can be added to a tea extract as a nitrogen source. The fermentation temperature is preferably at least 10 ° C, more preferably at least 15 ° C, even more preferably at least 28 ° C, preferably at most 45 ° C, more preferably at most 40 ° C, even more preferably at most 38 ° C. The pH of the tea extract is preferably pH 3.0 to 12.5, more preferably pH 3.5 to 10.0, and even more preferably pH 4.0 to 7.0. The fermentation time is not unconditionally determined according to the fermentation temperature or the sample amount, but is, for example, 16 to 96 hours. By fermenting the tea extract with a bacterium belonging to Lactobacillus pentosus, Awabancha, a traditional microbial fermented tea, is pickled from tea leaves in a barrel and spontaneously fermented. Although a preparation time of about a week was required, the time can be significantly reduced, and the productivity is improved.

  Thus, by fermenting the tea extract with a bacterium belonging to Lactobacillus pentosus as described above, 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''- A composition containing trihydroxyphenyl) -propan-2-ol can be obtained.

  The content of the fermented catechin of the present invention in the obtained fermented catechin-containing composition, the type of tea extract and bacteria, fermentation conditions, can not be unconditionally set by the subsequent treatment conditions and the like described below, preferably 0.13 It is at least mg / mL, more preferably at least 0.5 mg / mL, even more preferably at least 1.0 mg / mL.

  In the present invention, the metabolism of epigallocatechin to the fermented catechin of the present invention is promoted by bacteria belonging to Lactobacillus pentosus. The ratio (epigallocatechin / fermented catechin) is preferably 50/50 or less, more preferably 40/60 or less, still more preferably 30/70 or less, further preferably 20/80 or less, and still more preferably 10/90 or less. Further, metabolism may be promoted to such an extent that epigallocatechin is not substantially contained. In the present specification, `` substantially not contained '' means, for example, that the content is 0.01 mg / mL or less, as long as the content is at or below the detection limit in the measurement method described in Examples below. .

  Post-fermented tea leaves are known to have significantly reduced epigallocatechin-3-O-gallate compared to green tea leaves, but in the present invention, the decrease in epigallocatechin-3-O-gallate is relatively slow. Yes, the effect as epigallocatechin-3-O-gallate can be expected. The content of epigallocatechin-3-O-gallate in the fermented catechin-containing composition of the present invention is preferably 0.16 mg / mL or more, more preferably 0.5 mg / mL or more, and still more preferably 1.0 mg / mL or more.

  In the fermented catechin-containing composition of the present invention, the content ratio by weight of fermented catechin and epigallocatechin-3-O-gallate (fermented catechin / epigallocatechin-3-O-gallate) is preferably 20/80 or more, more preferably It is 40/60 or more, more preferably 45/55 or more.

  The fermented catechin-containing composition of the present invention contains, in addition to the fermented catechin and the compounds described above, caffeine, gallic acid, epicatechin, epicatechin-3-O-gallate, catechin, gallocatechin, gallocatechin-3-O-gallate, pyrogallol, and the like. May be. Their content is not particularly limited as long as the effects of the present invention are not impaired. In the present specification, the compound represented by the formula (I), epigallocatechin-3-O-gallate, epigallocatechin, and other compounds can be measured according to a measurement method described in Examples described later.

  Further, for the obtained fermented catechin-containing composition of the present invention, one or more selected from the group consisting of filtration, centrifugation, concentration, ultrafiltration, lyophilization, powderization, and fractionation The treatment may be performed according to a known method, or may be formulated as it is, or may be prepared in a form that can be used for raw materials such as food and drink (health food). Therefore, the processed product is included as one embodiment of the fermented catechin-containing composition of the present invention.

  As one embodiment of the present invention, the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature of the present invention is a food or drink. In another aspect, the present invention relates to a food or drink using the fermented catechin-containing composition of the present invention obtained by the production method of the present invention as a raw material.

  In the case of a food or drink using the fermented catechin-containing composition of the present invention as a raw material, the method for producing the same is not particularly limited as to the amount of the fermented catechin-containing composition used, the timing of addition, and the method of addition. Can be used without limitation, and the amount used and the method of addition can be appropriately selected according to known techniques. Specifically, for example, carriers, bases, and / or additives commonly used in the field of formulation, food, and the like can be appropriately compounded within a range that achieves the object of the present invention. Moreover, you may prepare by adding and dissolving and / or suspending the fermented catechin containing composition of this invention to a ready-made well-known food and drink. It should be noted that the known food or beverage may originally contain the compound represented by the formula (I).

  In the food and drink of the present invention, the content of 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol, Usually, it is about 0.00002 to 100% by weight, preferably about 0.0002 to 30% by weight, more preferably about 0.002 to 5% by weight.

  The composition for enhancing energy metabolism of the present invention, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the food or drink that is the composition for increasing body temperature, and the production method of the present invention The form of the obtained food or drink is not particularly limited, and for example, carbonated drinks, natural juices, fruit drinks, soft drinks (including fruit juices), pulp drinks, fruit foods containing grains, vegetable drinks, soy milk / soy milk Drinks, coffee drinks, tea drinks, jelly drinks, powdered drinks, concentrated drinks, sports drinks, nutritional drinks, favorite drinks such as alcoholic drinks, nutritional foods, supplements, pills, hard capsules, soft capsules, tablets (uncoated tablets, Sugar-coated tablets, rapidly disintegrating tablets in the mouth, chewable tablets (chewable tablets), effervescent tablets, troches, film-coated tablets and the like]. In addition, these may be those obtained by adding the fermented catechin-containing composition of the present invention to an existing food or drink at the time of or after the preparation, and there is no particular limitation on the timing and method of addition.

  The food or drink obtained by the production method of the present invention is the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature of the present invention. Like a certain food or drink, it contains the compound represented by the formula (I). Therefore, the food or drink obtained by the production method of the present invention enhances sympathetic nervous activity, for example, enhances energy metabolism, promotes fat burning, suppresses weight gain or reduces body weight, increases body temperature, and the like. Foods and drinks obtained by the production method of the present invention can be provided with a composition for enhancing energy metabolism, a composition for enhancing sympathetic nervous activity, and a composition for promoting fat burning, depending on the purpose, since they can exert physiological effects. , An anti-obesity composition and a body temperature increasing composition. Therefore, the present invention also provides a food or drink comprising the fermented catechin-containing composition of the present invention as a food or drink obtained by the method for producing a food or drink of the present invention. In this specification, “energy metabolism” means a process of converting energy released when ATP is converted to ADP into muscle contraction, nerve transmission, substance composition, body temperature regulation, etc. It is calculated as energy consumption from the amount and carbon dioxide emission. “Sympathetic nerve” means a sympathetic nerve in brown adipose tissue, which is a heat-producing tissue. When sympathetic nerve activity is increased, energy metabolism is increased, leading to an increase in body temperature, fat burning, and eventually anti-obesity. “Fat burning” uses a respiratory quotient, which is a volume ratio of carbon dioxide emission to oxygen consumption, as an index, and means that the lower the respiratory quotient, the more fat is burned. "Anti-obesity" means that weight gain is suppressed or weight is reduced.

  The food or drink obtained by the production method of the present invention is the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the composition for increasing body temperature of the present invention. Like certain foods and drinks, it is suitably used for, for example, symptoms requiring an action of enhancing energy metabolism, an action of increasing body temperature, an action of burning fat, and an anti-obesity action. Specifically, for example, it is suitably used for increasing body temperature, improving chilliness, anti-obesity, reducing visceral fat, beauty, promoting metabolism, stimulating immunity, anti-fatigue, antidepressant, and the like.

  Further, the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the food or drink that is the composition for increasing body temperature of the present invention, and the production of the present invention The food or drink obtained by the method has an effect of enhancing sympathetic nervous activity, which is extremely useful for improving and preventing symptoms requiring an action of increasing energy metabolism, an action of increasing body temperature, an action of burning fat, and further exerting an anti-obesity action. Useful. That is, the composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the food or drink which is the composition for increasing body temperature of the present invention, and the production of the present invention Food and drink obtained by the method, as a health food or health food with the aim of preventing or ameliorating the above symptoms, or 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 ``, 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as a food with a functional claim on the function possessed, for example, not only individuals who need to increase energy metabolism, energy metabolism Although not low, individuals who need to suppress weight gain, individuals who need to suppress chilling, individuals who need to suppress an increase in visceral fat, individuals who need to suppress a decrease in metabolism, and colds It is necessary to maintain individuals who want to treat or prevent A very useful composition for individuals such individuals, that need suppress fatigue to.

  The composition for enhancing energy metabolism of the present invention, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the food or drink that is the composition for increasing body temperature, and the production method of the present invention It is assumed that the obtained food or drink can indicate its use. The contents of the label, for example, lactic acid bacteria fermented product or 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol Uses derived from the functions of The indication may be attached to the food or beverage itself, or may be attached to a container or packaging of the food or beverage.

  The composition for enhancing energy metabolism of the present invention, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, or the food or drink that is the composition for increasing body temperature, and the production method of the present invention The intake of the obtained food or drink is not fixed as appropriate depending on their form, the method of ingestion, the purpose of ingestion, and the age, weight, and symptoms of the human being the subject of the food or drink, for example, 1- ( 3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol per day in humans weighing 50 kg, preferably 1 mg to 10 g And more preferably about 10 mg to 3 g, and still more preferably about 100 mg to 1 g. In addition, within the desired intake range, it may be used once or multiple times within one day, and the use time and duration are arbitrary.

  Hereinafter, the present invention will be described specifically with reference to Examples, but the present invention is not limited to the following Examples.

Example 1
Fermentation of tea extract using TUA4337L strain to give 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol Generated. Specifically, it is as follows. In addition, 1- (3 ′, 4 ′, 5′-trihydroxyphenyl) -3- (2 ″, 4 ″, 6 ″ -trihydroxyphenyl) -propan-2-ol is referred to as fermented catechin.
<Fermentation procedure>
(1) 1% v / v inoculation of TUA4337L from glycerol stock to MRS (10mL) (2) Culture (30 ° C, 20hr)
(3) After centrifugation (8,000 rpm, 5 min, 4 ° C), remove the supernatant (4) Wash with sterile water of the same volume as the culture solution (x 2)
(5) Bacterial solution was prepared using sterile water so that OD ₆₆₀ = 10. (6) 10% w / v of tea powder (manufactured by Cargill Japan) was added to sterile water, suspended, and then centrifuged (8,000 rpm, After 5 min), 0, 0.04, 0.2, 1 w / v% of yeast extract (manufactured by BD) was added to the supernatant (Bx 11.9), and the bacterial solution prepared in (5) was added at 1 v / v% (7). Culture (35 ℃)

The obtained culture solution was diluted with pure water, further diluted 2-fold with 40% acetonitrile, treated with a 0.45 μm filter, and subjected to the following analysis. FIG. 1 shows a representative chart of HPLC, and FIG. 2 shows the results of fermented catechin production.
<HPLC analysis conditions>
Column: capcell pak C18, 4.6mmφ × 150mm, 5μm (made by Shiseido)
Mobile phase: A 0.05% TFA / H ₂ O, B 90% CH ₃ CN, 0.05% TFA / H ₂ O
Gradient: B5 → 35v / v% (0 → 13min), B35 → 70v / v% (13 → 20min), B70v / v% (20 → 25min)
Flow rate: 1.0mL / min
Detection: A225nm
Injection volume: 10 μL

  1 and 2, by fermenting the tea extract using TUA4337L, epigallocatechin (EGC) decreased over time and fermented catechin was produced. Also, the addition of yeast extract increased the production rate. NMR confirmed that the new peak of C in FIG. 1 was fermented catechin.

Example 2
The production of fermented catechins by various strains belonging to lactic acid bacteria was examined.

  Using various L. pentosus and L. plantarum strains as lactic acid bacteria, the productivity of fermented catechin was examined together with the JCM reference strain. As L. pentosus, TUA4337L, AF43, AF48, AF51, AF64, JCM reference strain 1558T, and as L. plantarum, AF70, AF71, AF62, AF63, JCM reference strain 1149T were examined. Using these lactic acid bacteria, the productivity of fermented catechin was evaluated in the same procedure as in Example 1 (examined using tea extract to which 0 w / v% and 1 w / v% of yeast extract was added). Specifically, the concentration of fermented catechin in the culture solution after culturing for 72 hours was quantified. The results are shown in FIG.

  From FIG. 3, L. pentosus produced fermented catechin regardless of the strain, while L. plantarum failed to produce fermented catechin in any strain.

Example 3
The effect of the fermented catechin obtained in Example 1 on enhancing the sympathetic nervous activity of brown adipose tissue was examined. The fermented catechin obtained in Example 1 was obtained by isolating and concentrating the peak fraction of fermented catechin from the culture solution in Example 1, and then precipitating by freeze-drying.

  Specifically, for the experiment, three Wistar male rats (approximately 9 weeks old, n = 3 in each group) weighing about 300 g, bred for 1 week under a 12-hour light / dark cycle (lights on for 12 hours from 8:00). Urethane anesthesia was performed by fasting for a period of time, and a cannula for duodenal administration was inserted. Thereafter, the centrifugal branch of the sympathetic nerve of the interscapular brown adipose tissue was lifted with a silver electrode, and the electrical activity of the nerve was measured. At the time when the measured value of this nerve was settled (around 13:00), 1 mL of each test sample (control: sterilized water, fermented catechin: 1 μg / mL aqueous dispersion) was administered to the duodenum via a cannula, and the sympathetic nerve of brown adipose tissue was administered. Changes in activity were measured electrophysiologically. FIG. 4 shows the results.

  From FIG. 4, the BAT-SNA value showed a significant increase in fermented catechin as compared to the control. Therefore, fermented catechin was thought to enhance sympathetic activity in brown adipose tissue. Further, it was considered that the enhancement of the sympathetic nervous activity was a mechanism for enhancing the energy metabolism, and thus the proof was performed below.

Example 4
The effects of the fermented catechin obtained in Example 1 on enhancing energy metabolism and promoting fat burning were examined. The fermented catechin used was prepared in the same manner as in Example 3.

Specifically, in C57BL / 6J mice (7-week-old, male), oral administration of 250 μL of sterile water was continued for 5 days to familiarize themselves with oral administration. Thereafter, the animals were divided into a control group and a fermented catechin group so that there was no difference in the average value of body weight (each n = 4). After placing the mice in the chamber of an energy metabolism measuring device (Oxymax, Bio Research Center) for 2 days to familiarize with the environment, the diet was switched to a high-fat diet (60 kcal% FAT, Research Diet) and the oxygen of each mouse was changed. Measurement of consumption (VO ₂ (mL / kg / hr)) and carbon dioxide emission (VCO ₂ (mL / kg / hr)) was started. Each mouse was orally administered with 250 μL of a test sample per day (control: sterile water, fermented catechin: 5 mg / mL aqueous dispersion), and continued administration for 11 days. The calorie consumption and respiratory quotient were calculated by the following formulas.
Calorie consumption: VO ₂ × (RER × 1.232 + 3.815) / 1000
Here, RQ (respiratory quotient) = VCO ₂ / VO ₂

  The administration of fermented catechin resulted in an increase in calorie consumption as compared to the control group. The calorie consumption up to 11 days was compared, and data before administration and on day 4 are shown in FIG. This is the result of analyzing the calorie consumption during the light period (resting period: AM7: 00 to PM19: 00), and the calorie consumption significantly increased in the fermented catechin group. Since this is an effect during the resting period, it is considered that it indicates that basal metabolism was improved by fermented catechin, and it was confirmed that the fermented catechin had an energy metabolism-enhancing effect. In addition, respiratory quotient was measured to verify the change in fat burning effect before and after administration of fermented catechin. The respiratory quotient is the volume ratio of carbon dioxide output to oxygen consumption until nutrients are decomposed and converted into energy in a living body at a certain time. It is widely used as an index for understanding the outline of the rate at which nutrients are burned in the body. That is, the respiratory quotient reflects the energy source consumed, with carbohydrates (RQ = 1.0) generally having the highest respiratory quotient and lipids (RQ ≒ 0.7) having the lowest respiratory quotient. That is, when a large amount of fat is burned, the amount of carbon dioxide emitted is small relative to the amount of oxygen consumed, and the value of the respiratory quotient decreases. It is considered that the lower this value is, the more fat is burned, and the higher the fat burning effect is. FIG. 6 shows the difference in the respiratory quotient before administration and on the 11th day of administration. Compared with the control group, when the fermented catechin was administered, the respiratory quotient was lower and the fat burning effect was promoted Conceivable.

Example 5
The effect of increasing body temperature by the fermented catechin obtained in Example 1 was examined. The fermented catechin used was prepared in the same manner as in Example 3.

  Specifically, C57BL / 6J mice (10-week-old, male) bred on a high-fat diet (60 kcal% FAT) for 2 weeks were tested with 250 μL of test sample per day (control: sterilized water, fermented catechin: 1.6%). mg / mL aqueous dispersion) were orally administered (each n = 11-12). Five and 60 minutes after oral administration, the rectal temperature was measured using a microprobe thermometer (BAT-12, muromachi), and the range of change in rectal temperature was calculated. FIG. 7 shows the results.

  As shown in FIG. 7, the fermented catechin significantly increased the rectal temperature compared to the control. Therefore, fermented catechin is considered to have a body temperature increasing effect.

Example 6
The anti-obesity effect of the fermented catechin obtained in Example 1 was examined. The fermented catechin used was prepared in the same manner as in Example 3.

  Specifically, C57BL / 6J mice (7 weeks old, male) were bred on a high fat diet (60 kcal% FAT) for 2 weeks. During that time, oral administration of 250 μL of sterile water was continued for 5 days, and the patient was used to oral administration. Thereafter, the animals were divided into a control group and a fermentation catechin-administered group so that there was no difference in the average value of body weight (n = 4 each). Each mouse was orally administered with 250 μL of a test sample per day (control: sterile water, fermented catechin: 5 mg / mL aqueous dispersion), and the body weight was measured over time. FIG. 8 shows changes in weight gain.

  FIG. 8 shows that there is a tendency that weight gain is suppressed by administering fermented catechin, and fermented catechin is considered to have an anti-obesity effect. As an action mechanism, an energy metabolism enhancing action can be considered similarly to the body temperature increasing effect.

  Hereinafter, specific formulations of the composition of the present invention will be exemplified. These compositions can be prepared according to known methods.

Prescription example: Green tea beverage 1
After the culture solution of Example 1 was dried, it was mixed with 9 times the amount of dextrin to prepare a fermented catechin mixture. The mixture and powdered tea were added to water, mixed, and sterilized at 121 ° C. for 20 minutes to prepare a green tea beverage. I do.

Formulation example: coffee beverage About 10 weight times of boiling water was added to 200 g of coffee beans (L value: 27) to obtain Bx. 2000 g of the coffee extract of 2.6 are obtained. This extract is mixed with the mixture prepared for the above green tea beverage, granulated sugar, milk, and a pH adjuster to prepare a coffee beverage.

Formulation example: carbonated beverage After dissolving the mixture prepared for the above green tea beverage in water, the pH is adjusted to 3.8 with phosphoric acid, and an antioxidant, a flavor, an acidulant, a sweetener, and a caramel pigment are added. Add the appropriate amount of each and store for about 24 hours. During that time, an appropriate amount of carbon dioxide gas is added, and then a carbonated beverage is obtained through the steps of filtration, bottling, and sterilization (heating at 65 ° C. or higher for 10 minutes).

Prescription example: Green tea beverage 2
The fermented catechin was isolated from the culture solution of Example 1, dried, and then mixed with 9 times the amount of dextrin to prepare a fermented catechin mixture. The mixture and powdered tea were added to water, mixed, and then sterilized at 121 ° C for 20 minutes. To prepare a green tea beverage.

Therefore, the present invention includes the following embodiments.
[1] An energy metabolism enhancer containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 ", 4", 6 "-trihydroxyphenyl) -propan-2-ol as an active ingredient .
(2) a composition containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient Energy metabolism enhancer.
[3] Sympathetic nervous activity enhancement using 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient Agent.
(4) a composition containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient Sympathetic activity enhancer.
[5] Fat burning promoter containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 ", 4", 6 "-trihydroxyphenyl) -propan-2-ol as an active ingredient .
(6) a composition containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient Fat burning promoter.
[7] An anti-obesity agent comprising 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 ", 4", 6 "-trihydroxyphenyl) -propan-2-ol as an active ingredient.
(8) a composition containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient Anti-obesity agent.
[9] A body temperature increasing agent comprising 1- (3 ′, 4 ′, 5′-trihydroxyphenyl) -3- (2 ″, 4 ″, 6 ″ -trihydroxyphenyl) -propan-2-ol as an active ingredient.
(10) A composition containing 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient Body temperature increasing agent.

  The composition for enhancing energy metabolism, the composition for enhancing sympathetic nervous activity, the composition for promoting fat burning, the composition for anti-obesity, and the composition for increasing body temperature of the present invention have excellent energy metabolism enhancing action and sympathetic nervous activity. It has an accelerating effect, a fat burning promoting effect, an anti-obesity (suppression of weight gain or weight loss) effect, and a body temperature increasing effect. For example, it has an effect of increasing body temperature, improving coldness, anti-obesity, reducing visceral fat, beauty, Effects such as metabolism promotion, immunostimulation, anti-fatigue and antidepressant can be expected. Further, 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol-containing composition obtained by the production method of the present invention. The substance has an excellent action of enhancing energy metabolism, an action of enhancing sympathetic nervous activity, an action of promoting fat burning, an action of suppressing or decreasing weight gain, and an action of increasing body temperature. Improvement, anti-obesity, visceral fat reduction, beauty, promotion of metabolism, immunostimulation, anti-fatigue, antidepressant, and other effects can be expected.

Claims (5)

1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient, a composition for enhancing energy metabolism (However, tea leaves of Awabancha and Goishicha, extracts of the tea leaves, and compositions containing these are excluded) . 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as active ingredient, composition for enhancing sympathetic nervous activity (Excluding tea leaves of Awabancha and Goishi tea, extracts of the tea leaves, and compositions containing these) . 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient, composition for promoting fat burning (However, tea leaves of Awabancha and Goishicha, extracts of the tea leaves, and compositions containing these are excluded) . An anti-obesity composition comprising 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient ( However, tea leaves of Awabancha and Goishicha, extracts of the tea leaves, and compositions containing these are excluded) . 1- (3 ', 4', 5'-trihydroxyphenyl) -3- (2 '', 4 '', 6 ''-trihydroxyphenyl) -propan-2-ol as an active ingredient, a composition for increasing body temperature ( However, tea leaves of Awabancha and Goishicha, extracts of the tea leaves, and compositions containing these are excluded) .

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