JP6595157B2 - Cell imaging control apparatus and method, and program - Google Patents

Description

  The present invention relates to a cell imaging control apparatus, method, and program for controlling imaging of a cell image.

  Conventionally, a method has been proposed in which stem cells such as ES cells and iPS cells, differentiation-induced cells, and the like are imaged over time, and the culture state of the cells is determined by capturing changes over time in the images (for example, (See Patent Document 1 and Patent Document 2).

  Further, Patent Document 3 proposes a method that enables a more detailed observation by specifying the position of a cell colony from an image captured at a low magnification and capturing the identified cell colony at a high magnification. .

JP 2011-229410 A JP2013-236564A JP 2012-002949 A JP 2013-109119 A Japanese Patent No. 5145487

  However, in the methods described in Patent Document 1 and Patent Document 2, when a highly accurate determination is to be made, it is necessary to image the entire dish or the entire cell region in time series with high magnification, and data to be processed and stored The amount of will become enormous. In the method described in Patent Document 3, the position of a cell colony can be specified by an image captured at a low magnification, and only the identified cell colony can be captured at a high magnification. It is necessary to image all of the cell colonies that have been processed at a high magnification, and the amount of data to be processed and stored is enormous.

  Patent Document 4 describes narrowing down cell colonies for detailed observation at high magnification based on morphological characteristics of cell colonies, and Patent Document 5 describes based on the size of cell colonies. In addition, it is described to narrow down cell colonies for performing detailed observation at high magnification.

  However, since changes in morphological characteristics and size of cell colonies are not clear unless the culture period is relatively long, the methods described in Patent Document 4 and Patent Document 5 show the observation region in a relatively short culture period. Cannot narrow down. Moreover, since it cannot narrow down to an appropriate observation area | region, there exists a problem that the precision of the determination based on the cell image also worsens.

  Further, in the methods described in Patent Document 4 and Patent Document 5, it is possible to narrow down in units of cell colonies, but it is not possible to narrow down to a local region in the cell colonies and perform detailed observation.

  In addition, when cell colonies grow and combine with other cell colonies, changes in morphological characteristics and changes in size are different from those of single cell colonies, making it difficult to narrow down to an appropriate observation area. It is.

  In view of the above problems, an object of the present invention is to provide a cell imaging control apparatus, method, and program capable of appropriately setting a region of interest for performing high-magnification imaging even in a relatively short culture period. To do.

  The cell imaging control device of the present invention sets a plurality of partial areas in a low-magnification image acquisition unit that acquires images obtained by imaging cells at a first magnification in time series, and each partial area. A growth rate acquisition unit that acquires cell growth rates based on a plurality of images captured in time series, and an attention area that determines a region of interest from a plurality of partial regions based on the growth rates of each partial region An area determination unit and an imaging control unit that controls to capture only a region of interest among a plurality of partial regions at a second magnification higher than the first magnification are provided.

  Further, the attention area determination unit can determine an abnormal area or a normal area as the attention area.

  Further, the attention area determination unit can determine a partial area whose growth rate is greater than or equal to a threshold value or less as a normal area.

  Further, when the cell is a stem cell, the attention area determination unit can determine the undifferentiated cell area as a normal area.

  Further, when the cell is a differentiation-induced cell, the attention area determination unit can determine the differentiated cell area as a normal area.

  Further, the attention area determination unit can determine a partial area whose growth rate is equal to or higher than a threshold value or lower than a threshold value as an abnormal area.

  In addition, when the cell is a stem cell, the attention area determination unit can determine the differentiated cell area as an abnormal area.

  Further, when the cell is a differentiation-induced cell, the attention area determination unit can determine the undifferentiated cell area as an abnormal area.

  The growth rate acquisition unit can set an area obtained by dividing a colony area of cells included in the image area as the partial area.

  The cell imaging control method of the present invention acquires an image obtained by imaging a cell at a first magnification in time series, sets a plurality of partial areas in the area of the image, and images each of the partial areas in time series. Cell proliferation rates are obtained based on the plurality of images, and a region of interest is determined from the plurality of partial regions based on the growth rate of each partial region. Control is performed so that an image is captured at a second magnification higher than the first magnification.

  In the cell imaging control program of the present invention, a computer sets a plurality of partial areas in an image area, a low-magnification image acquisition unit that acquires an image obtained by imaging cells in time series at a first magnification, For a partial region, a growth rate acquisition unit that acquires cell growth rates based on a plurality of images captured in time series, and a region of interest from a plurality of partial regions based on the growth rate of each partial region An attention area determination unit to be determined, and an imaging control unit that controls to image only the attention area of the plurality of partial areas at a second magnification higher than the first magnification are characterized.

  According to the cell imaging control device, method, and program of the present invention, an image obtained by imaging a cell at a low magnification is acquired, a plurality of partial areas are set in the area of the image, and the image of each partial area is obtained. Since each cell growth rate was acquired and the region of interest for high-magnification imaging was determined from the multiple partial regions based on the growth rate of each partial region, even during a relatively short culture period It is possible to appropriately set the attention area of the high magnification imaging target.

The block diagram which shows schematic structure of the cell culture observation system using one Embodiment of the cell imaging control apparatus of this invention. Diagram for explaining how to set a partial area Diagram for explaining other methods for setting partial areas The flowchart for demonstrating the effect | action of the cell culture observation system using one Embodiment of the cell imaging control apparatus of this invention.

  Hereinafter, a cell culture observation system using an embodiment of a cell imaging control apparatus and method and a program of the present invention will be described in detail with reference to the drawings. FIG. 1 is a block diagram showing a schematic configuration of a cell culture observation system.

  As shown in FIG. 1, the cell culture observation system includes a cell culture device 1, an imaging device 2, a cell imaging control device 3, a display 4, and an input device 5.

  The cell culture device 1 is a device for culturing cells. Examples of cells to be cultured include stem cells such as iPS cells and ES cells, cells such as nerves, skin and liver induced by differentiation from stem cells, and cancer cells. In the cell culture apparatus 1, a plurality of culture containers in which cells to be cultured are seeded in a medium are accommodated. The cell culture device 1 includes a stage 10, a transport unit 11, and a control unit 12.

The stage 10 is provided with a culture container to be imaged by the imaging device 2. In addition, the transport unit 11 selects a culture container to be imaged from a plurality of culture containers accommodated at a predetermined position in the cell culture apparatus 1, and transports the selected culture container to the stage 10. Further, the control unit 12 controls the entire cell culture apparatus 1, and in addition to the operations of the stage 10 and the transfer unit 11 described above, environmental conditions such as temperature, humidity, and CO ₂ concentration in the cell culture apparatus 1 are controlled. It is something to control. The temperature, the configuration for adjusting the humidity and CO ₂ concentration can be a known configuration.

  The imaging device 2 captures images of cells in a culture container installed on the stage 10 in time series. The imaging device 2 includes an optical system 20 for imaging and acquiring an image of a cell, an imaging element 21 that photoelectrically converts an image imaged by the optical system 20 and outputs the image signal, an optical system 20, And a control unit 22 that controls the image sensor 21.

  As the optical system 20, for example, a phase contrast microscope or a differential interference microscope can be used. Further, as the image sensor 21, a CMOS (Complementary Metal-Oxide Semiconductor) sensor, a CCD (charge-coupled device) sensor, or the like can be used.

  The control unit 22 controls the entire image pickup apparatus 2. In particular, in the present embodiment, the control unit 22 controls the magnification of the optical system 20 and the position of the image pickup region. Then, the control unit 22 sets the magnification of the optical system 20 to a low magnification of about 1 to 4 times and 10 to 20 based on a control signal output from the imaging control unit 33 in the cell imaging control device 3 described later. Switching to a high magnification of about double and controlling the position of the imaging region in accordance with the switching of the magnification will be described in detail later. In the present embodiment, as described above, the magnification of the optical system 20 is automatically switched based on the control signal output from the imaging control unit 33. However, the present invention is not limited to this, and the user manually performs the magnification. May be switched.

  The cell imaging control device controls imaging conditions in the imaging device 2. Specifically, in the imaging device 2, first, the cells are imaged at a low magnification, cell proliferation rates are acquired for a plurality of partial regions in the image captured at the low magnification, and based on the proliferation rates. A region of interest is determined from a plurality of partial regions, and the imaging device 2 is controlled to capture an image of the region of interest at a high magnification.

  The cell imaging control apparatus 3 is obtained by installing an embodiment of the cell imaging control program of the present invention on a computer.

  The cell imaging control device 3 includes a central processing unit, a semiconductor memory, a hard disk, and the like, and an embodiment of a cell imaging control program is installed on the hard disk. Then, when this program is executed by the central processing unit, the image acquisition unit 30, the growth rate acquisition unit 31, the attention area determination unit 32, the imaging control unit 33, and the display control unit 34 as shown in FIG. 1 operate. .

  The image acquisition unit 30 acquires and stores a plurality of images (hereinafter, referred to as low-magnification images) captured in time series at a low magnification (first magnification) by the imaging device 2, and at the same time a high magnification by the imaging device 2 An image of a region of interest (hereinafter referred to as a high magnification image) captured at (second magnification) is acquired and stored. The image acquisition unit 30 outputs the acquired low-magnification image to the growth rate acquisition unit 31, and outputs the low-magnification image and the high-magnification image to the display control unit 34.

  The growth rate acquisition unit 31 sets a plurality of partial regions in the region of the low-magnification image, and the proliferation rate of cells in the partial region based on the low-magnification images captured in time series for each partial region. Are obtained.

  As a partial region setting method, for example, as shown in FIG. 2, the partial region may be set by dividing an imaging region obtained by imaging one stem cell corow into a plurality of rectangular regions. In addition, as shown in FIG. 2, each partial area may be divided and set so as not to overlap, or may be set so that a part overlaps between adjacent partial areas. Good.

  As for the growth rate, for example, the growth rate acquisition unit 31 counts the number of cells in each corresponding partial region in a plurality of low magnification images taken at different times, and the increase in the number of cells and the low magnification image It may be calculated based on the imaging interval.

  Regarding the method of detecting cells in each partial region, for example, cell edges may be detected and individual cells may be detected using pattern matching or the like, or intracellular nuclei or nucleoli are detected. Thus, individual cells may be detected, and other known methods can be used.

  In the above description, the partial area is set by dividing the imaging area in which one stem cell color is imaged into a plurality of rectangular areas. However, the partial area setting method is not limited to this. For example, FIG. As shown in FIG. 3, a plurality of stem cell colony regions included in the imaging region may be extracted, and the extracted stem cell colony regions may be set as partial regions.

  The attention area determination unit 32 determines an attention area from among a plurality of partial areas based on the proliferation speed of each partial area acquired by the proliferation speed acquisition section 31.

  Specifically, for example, when the cell to be imaged is a stem cell such as an iPS cell or ES cell, and the undifferentiated cell region is a stem cell having a property that the proliferation rate is higher than that of the differentiated region, the proliferation rate is determined in advance. A partial region that is equal to or greater than a set threshold value may be determined as a region of interest as an undifferentiated cell region and a normal region. Alternatively, a partial region whose growth rate is equal to or lower than a preset threshold value may be determined as a region of interest as a differentiated cell region and an abnormal region.

  In addition, when the cell to be imaged is a stem cell having a property that the differentiated cell region has a faster growth rate than the undifferentiated cell region, the partial region in which the growth rate is equal to or less than a preset threshold is determined as undifferentiated. The region of interest may be determined as being a cell region and a normal region. Alternatively, a partial region having a growth rate equal to or higher than a preset threshold value may be determined as a region of interest as a differentiated cell region and an abnormal region.

  In addition, when the cell to be imaged is a cell in which differentiation has been induced and the differentiated cell region has a property that the proliferation rate is higher than that of the undifferentiated cell region, the proliferation rate is equal to or higher than a preset threshold value. When there is a partial area, the partial area is a differentiated cell area that has been differentiated into a target cell such as skin, for example, and may be determined as an attention area as a normal area. Conversely, when there is a partial region whose proliferation rate is equal to or lower than a preset threshold, the partial region is an undifferentiated cell region that has not been induced to differentiate into the target cell, and is considered as an abnormal region. You may make it decide to.

  In addition, for example, when the cell to be imaged is a differentiation-induced cell, and the undifferentiated cell region is a cell having a property that the proliferation rate is faster than the differentiated region, the proliferation rate is set to a preset threshold value. The partial region described above may be determined as a region of interest as an undifferentiated cell region and an abnormal region. Alternatively, a partial region whose growth rate is equal to or less than a preset threshold value may be determined as a region of interest as a differentiated cell region and a normal region.

  Note that, as described above, the attention area determination unit 32 determines the attention area by comparing the growth rate with a threshold value, and this threshold value is changed according to the cell type, the culture condition, and the culture period. May be. The threshold may be increased as the cell grows faster, the threshold may be increased as the culture conditions grow faster, or the threshold may be decreased as the culture period is longer. The threshold value corresponding to the cell type, culture condition, or culture period may be set in advance using, for example, a table. Moreover, what is necessary is just to make it a user input using the input device 5 about the kind of cell, culture | cultivation conditions, or a culture | cultivation period.

  The imaging control unit 33 controls the imaging conditions in the imaging apparatus 2 by outputting a control signal to the imaging apparatus 2. In particular, the imaging control unit 33 of the present embodiment outputs a control signal to the control unit 22 of the imaging apparatus 2 to instruct to capture a low-magnification image for acquiring the growth rate, and then acquires the growth rate. Among the plurality of partial regions set in the unit 31, a control signal for instructing to image only the attention region determined in the attention region determination unit 32 at a high magnification is output to the control unit 22 of the imaging device 2. . In the present embodiment, as described above, when acquiring a low-magnification image, the magnification of the optical system 20 is controlled to about 1 to 4 times, and when acquiring a high-magnification image, the optical system 20 The magnification is controlled to about 10 to 20 times.

  In addition, the imaging control unit 33 outputs a control signal to the control unit 12 of the cell culture device 1 based on the attention area determined by the attention area determination unit 32 so that the attention area is imaged at a high magnification. The movement in the X-Y direction (the direction in the installation surface of the culture vessel) is controlled.

  The display control unit 34 receives the low-magnification image and the high-magnification image acquired by the image acquisition unit 30 and displays them on the display 4. In addition, the display control unit 34 may display the position (for example, coordinate value) of the partial area determined as the attention area, the number of the cell colony, and the like on the display 4. Further, the display control unit 34 has information indicating that it is a normal region, information indicating that it is an abnormal region, information indicating that it is an undifferentiated cell region, or the region of interest determined by the region of interest determination unit 32 Information indicating a differentiated cell region or the like may be displayed on the display 4.

  The input device 5 includes a mouse, a keyboard, and the like, and receives an operation input by a user. For example, the input device 5 can accept a setting input when capturing a magnification when capturing a low-magnification image or a high-magnification image. The input device 5 receives a setting input for the range of the partial region, and the setting input such as the above-described cell type, culture condition, and culture period.

  Next, the operation of the above-described cell culture observation system will be described with reference to the flowchart shown in FIG.

  First, in the cell culture apparatus 1, the culture to be imaged is selected from the plurality of accommodated culture containers by the transport unit 11, and the selected culture container is placed on the stage 10 (S10).

  Then, a low magnification imaging control signal is output from the imaging control unit 33 of the cell imaging control device 3 to the control unit 22 of the imaging device 2, and the control unit 22 of the imaging device 2 responds to the input control signal. The magnification of the optical system 20 is set to the above-described low magnification (first magnification), and so-called time-lapse imaging is performed to capture time-series low-magnification images (S12).

  A time-series low-magnification image captured by the imaging device 2 is acquired by the image acquisition unit 30 of the cell imaging control device 3 and output to the growth rate acquisition unit 31. The growth rate acquisition unit 31 sets a plurality of partial regions within the region of the input low-magnification image, and calculates a cell growth rate for each partial region (S14).

  And the growth rate of each partial area acquired in the growth rate acquisition part 31 is output to the attention area determination part 32, and the attention area determination part 32 is based on the input proliferation speed of each partial area. An attention area is determined from the partial areas (S16).

  Next, the position information of the attention area is output to the imaging control section 33, and the imaging control section 33 instructs to capture the attention area at the above-described high magnification (second magnification) based on the input position information. The control signal to be output is output to the control unit 22 of the imaging device 2 and the control unit 12 of the cell culture device 1. The control unit 22 of the imaging device 2 sets the magnification of the optical system 20 to the high magnification (second magnification) described above according to the input control signal, and the control unit 12 of the cell culture device 1 Is moved in the XY direction, so-called time-lapse imaging is performed, and a time-series high-magnification image is captured (S18).

  The high-magnification image acquired by the imaging device 2 is acquired by the image acquisition unit 30 of the cell imaging control device 3 and input to the display control unit 34. The display control unit 34 displays the input high-magnification image on the display 4. The user determines the state of the cell by observing the high-magnification image displayed on the display (S20).

  Note that the determination of the state of the cell based on the high-magnification image may be automatically performed in the determination unit provided in the cell imaging control device 3. In this case, for example, whether the stem cells are differentiated or undifferentiated can be determined from, for example, the density or luminance distribution of the cells in the high-magnification image, and other known methods may be used. Note that differentiation / undifferentiation may be determined based on the growth rate used when determining the region of interest.

  In addition, whether or not differentiation-induced cells have differentiated into target cells can be determined from, for example, morphological characteristics of the cells. Also in this case, it may be determined whether or not normal differentiation has occurred based on the growth rate used when determining the region of interest.

  According to the cell culture observation system of the above embodiment, an image obtained by capturing cells at a low magnification is acquired, a plurality of partial regions are set in the region of the image, and cell proliferation is performed based on the images of the partial regions. Since the speed is acquired and the attention area of the high-magnification imaging target is determined from a plurality of partial areas based on the growth rate of each partial area, high-magnification imaging is performed even during a relatively short culture period. The target region of interest can be set appropriately.

  In addition, when a region obtained by dividing a cell colony is set as a partial region, a local region in the cell colony can be set as a region of interest, thereby differentiating the local region in the cell colony. -Determination of undifferentiation can be performed with high accuracy.

DESCRIPTION OF SYMBOLS 1 Cell culture apparatus 2 Imaging apparatus 3 Cell imaging control apparatus 4 Display 5 Input apparatus 10 Stage 11 Conveyance part 12 Control part 20 Optical system 21 Imaging element 22 Control part 30 Image acquisition part 31 Growth rate acquisition part 32 Attention area determination part 33 Imaging Control unit 34 Display control unit

Claims (15)

A low-magnification image acquisition unit that acquires an image of cells imaged at a first magnification in time series;
A plurality of partial regions in the region of the image, and for each partial region, a proliferation rate acquisition unit that acquires the proliferation rate of the cells based on the plurality of images captured in time series, and
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
When the type of cell input by the user is a stem cell having a property that the proliferation rate of the undifferentiated cell region is faster than that of the differentiated cell region, the partial region whose proliferation rate is greater than or equal to the threshold is the undifferentiated cell region. A region of interest for determining a region of interest as a normal region, or a partial region having a growth rate equal to or lower than a threshold, a region of interest as a differentiated cell region and an abnormal region ;
A cell imaging control device comprising: an imaging control unit configured to control imaging of only the region of interest among the plurality of partial regions at a second magnification higher than the first magnification. A low-magnification image acquisition unit that acquires an image of cells imaged at a first magnification in time series;
A plurality of partial regions in the region of the image, and for each partial region, a proliferation rate acquisition unit that acquires the proliferation rate of the cells based on the plurality of images captured in time series, and
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
When the type of cell input by the user is a stem cell having a property that the differentiated cell region has a higher proliferation rate than the undifferentiated cell region, the partial region whose proliferation rate is equal to or less than the threshold value is the undifferentiated cell region. A region of interest for determining a region of interest as a normal region, or a partial region having a growth rate equal to or greater than a threshold, and a region of interest as a differentiated cell region and an abnormal region ;
A cell imaging control device comprising: an imaging control unit configured to control imaging of only the region of interest among the plurality of partial regions at a second magnification higher than the first magnification. A low-magnification image acquisition unit that acquires an image of cells imaged at a first magnification in time series;
A plurality of partial regions in the region of the image, and for each partial region, a proliferation rate acquisition unit that acquires the proliferation rate of the cells based on the plurality of images captured in time series, and
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
If the type of cell input by the user is a differentiation-induced cell that has a property that the differentiated cell region has a faster growth rate than the undifferentiated cell region, differentiate the partial region whose proliferation rate is equal to or greater than the threshold value. An attention area determination unit that determines a region of interest as a normal area as a cell area, or determines a partial area whose growth rate is equal to or less than a threshold as an attention area as an abnormal area and an abnormal area When,
A cell imaging control device comprising: an imaging control unit configured to control imaging of only the region of interest among the plurality of partial regions at a second magnification higher than the first magnification. A low-magnification image acquisition unit that acquires an image of cells imaged at a first magnification in time series;
A plurality of partial regions in the region of the image, and for each partial region, a proliferation rate acquisition unit that acquires the proliferation rate of the cells based on the plurality of images captured in time series, and
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
If the cell type entered by the user is a differentiation-induced cell in which the growth rate of the undifferentiated cell region is higher than that of the differentiated cell region, the partial region having a growth rate equal to or higher than the threshold value is not changed. A region of interest determination unit that determines a region of interest as a differentiated cell region and an abnormal region, or determines a partial region whose growth rate is equal to or less than a threshold as a region of interest as a differentiated cell region and a normal region When,
A cell imaging control device comprising: an imaging control unit configured to control imaging of only the region of interest among the plurality of partial regions at a second magnification higher than the first magnification. The growth rate acquiring unit, cell imaging control device colonies area of the cells contained within the region divided regions claim 1 is set as the said partial region 4 any one of claims of the image. The growth rate acquiring unit, on the basis of the number of cells contained in each of the plurality of images of partial areas, cell imaging control apparatus of claim 1 to 5 any one of claims to obtain the growth rate. The cell imaging control according to claim 6 , wherein the proliferation rate acquisition unit acquires the proliferation rate based on the number of cells included in each of the plurality of images of the partial region and the imaging interval of the plurality of images. apparatus. Obtain an image of cells imaged at a first magnification in time series,
Setting a plurality of partial areas in the area of the image, for each of the partial areas, respectively acquiring the proliferation rate of the cells based on the plurality of images taken in time series,
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
When the cell type input by the user is a stem cell having a property that the growth rate of the undifferentiated cell region is higher than that of the differentiated cell region, the partial region whose growth rate is equal to or higher than the threshold is the undifferentiated cell region. Determine a region of interest as a normal region, or determine a partial region whose proliferation rate is equal to or less than a threshold value as a region of differentiation and an abnormal region as a region of interest ,
A cell imaging control method, wherein control is performed so that only the region of interest among the plurality of partial regions is imaged at a second magnification higher than the first magnification. Obtain an image of cells imaged at a first magnification in time series,
Setting a plurality of partial areas in the area of the image, for each of the partial areas, respectively acquiring the proliferation rate of the cells based on the plurality of images taken in time series,
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
When the cell type input by the user is a stem cell having a property that the differentiated cell region has a higher proliferation rate than the undifferentiated cell region, the partial region whose proliferation rate is equal to or less than the threshold value is the undifferentiated cell region. To determine the region of interest as being a normal region, or to determine a partial region whose proliferation rate is equal to or greater than a threshold as a region of differentiation and an abnormal region as a region of interest ,
A cell imaging control method, wherein control is performed so that only the region of interest among the plurality of partial regions is imaged at a second magnification higher than the first magnification. Obtain an image of cells imaged at a first magnification in time series,
Setting a plurality of partial areas in the area of the image, for each of the partial areas, respectively acquiring the proliferation rate of the cells based on the plurality of images taken in time series,
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
If the type of cell input by the user is a differentiation-induced cell that has a property that the differentiated cell region has a faster growth rate than the undifferentiated cell region, differentiate the partial region whose proliferation rate is equal to or greater than the threshold value. Determine the region of interest as a normal region as a cell region, or determine a partial region whose growth rate is equal to or less than a threshold as the region of interest as an undifferentiated cell region and an abnormal region ,
A cell imaging control method, wherein control is performed so that only the region of interest among the plurality of partial regions is imaged at a second magnification higher than the first magnification. Obtain an image of cells imaged at a first magnification in time series,
Setting a plurality of partial areas in the area of the image, for each of the partial areas, respectively acquiring the proliferation rate of the cells based on the plurality of images taken in time series,
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
If the cell type entered by the user is a differentiation-induced cell in which the growth rate of the undifferentiated cell region is higher than that of the differentiated cell region, the partial region having a growth rate equal to or higher than the threshold value is not changed. Determine a region of interest as a differentiated cell region and an abnormal region, or determine a partial region whose growth rate is equal to or less than a threshold value as a region of differentiation and a normal region as a region of interest ,
A cell imaging control method, wherein control is performed so that only the region of interest among the plurality of partial regions is imaged at a second magnification higher than the first magnification. A computer, a low-magnification image acquisition unit that acquires an image obtained by imaging cells in time series at a first magnification;
A plurality of partial regions in the region of the image, and for each partial region, a proliferation rate acquisition unit that acquires the proliferation rate of the cells based on the plurality of images captured in time series, and
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
When the type of cell input by the user is a stem cell having a property that the proliferation rate of the undifferentiated cell region is faster than that of the differentiated cell region, the partial region whose proliferation rate is greater than or equal to the threshold is the undifferentiated cell region. A region of interest for determining a region of interest as a normal region, or a partial region having a growth rate equal to or lower than a threshold, a region of interest as a differentiated cell region and an abnormal region ;
A cell imaging control program that functions as an imaging control unit that controls to image only the region of interest of the plurality of partial regions at a second magnification higher than the first magnification. A computer, a low-magnification image acquisition unit that acquires an image obtained by imaging cells in time series at a first magnification;
A plurality of partial regions in the region of the image, and for each partial region, a proliferation rate acquisition unit that acquires the proliferation rate of the cells based on the plurality of images captured in time series, and
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
When the cell type input by the user is a stem cell having a property that the differentiated cell region has a higher proliferation rate than the undifferentiated cell region, the partial region whose proliferation rate is equal to or less than the threshold value is the undifferentiated cell region. A region of interest for determining a region of interest as a normal region, or a partial region having a growth rate equal to or greater than a threshold, and a region of interest as a differentiated cell region and an abnormal region ;
A cell imaging control program that functions as an imaging control unit that controls to image only the region of interest of the plurality of partial regions at a second magnification higher than the first magnification. A computer, a low-magnification image acquisition unit that acquires an image obtained by imaging cells in time series at a first magnification;
A plurality of partial regions in the region of the image, and for each partial region, a proliferation rate acquisition unit that acquires the proliferation rate of the cells based on the plurality of images captured in time series, and
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
If the type of cell input by the user is a differentiation-induced cell that has a property that the differentiated cell region has a faster growth rate than the undifferentiated cell region, differentiate the partial region whose proliferation rate is equal to or greater than the threshold value. An attention area determination unit that determines a region of interest as a normal area as a cell area, or determines a partial area whose growth rate is equal to or less than a threshold as an attention area as an abnormal area and an abnormal area When,
A cell imaging control program that functions as an imaging control unit that controls to image only the region of interest of the plurality of partial regions at a second magnification higher than the first magnification. A computer, a low-magnification image acquisition unit that acquires an image obtained by imaging cells in time series at a first magnification;
A plurality of partial regions in the region of the image, and for each partial region, a proliferation rate acquisition unit that acquires the proliferation rate of the cells based on the plurality of images captured in time series, and
In determining an abnormal region or a normal region as a region of interest from among the plurality of partial regions based on the growth rate of each partial region ,
If the cell type entered by the user is a differentiation-induced cell in which the growth rate of the undifferentiated cell region is higher than that of the differentiated cell region, the partial region having a growth rate equal to or higher than the threshold value is not changed. A region of interest determination unit that determines a region of interest as a differentiated cell region and an abnormal region, or determines a partial region whose growth rate is equal to or less than a threshold as a region of interest as a differentiated cell region and a normal region When,
A cell imaging control program that functions as an imaging control unit that controls to image only the region of interest of the plurality of partial regions at a second magnification higher than the first magnification.

Images