JP6497675B2 - Composition for reducing activity and / or anxiety - Google Patents

Description

  The present invention relates to a composition effective for reducing activity and / or improving anxiety.

  Reflecting the current aging and stressed society, there is a problem of decreased activity and increased anxiety.

  It is known that a decrease in activity reduces social productivity, and an increase in anxiety promotes the development of mental illness and progression of symptoms, and increases the risk of developing lifestyle-related diseases. There is a need for food and medicine.

  Oleuropein is known to have various effects such as skin protection, antioxidant, antibacterial and antiviral (Patent Document 1).

  Patent Document 2 describes that olives or extracts thereof are useful as endurance improvers, anti-fatigue agents, and motor function improvers, and Patent Document 3 discloses a composition containing hydroxytyrosol and other components. Describe how to increase energy generation capacity.

JP2014-9226 JP2009-161459 Special table 2012-524033

  The present inventor aims to provide a composition that is effective in reducing activity and / or improving anxiety.

The present invention provides the following composition for reducing activity and / or anxiety.
Item 1. A composition for reducing activity and / or anxiety comprising oleuropein.
Item 2. Item 2. The composition according to Item 1, wherein the cause of decreased activity or anxiety is a decrease in psychosomatic function due to aging.
Item 3. Item 2. The composition according to Item 1, wherein the cause of decreased activity or anxiety is a neuropsychiatric disorder.
Item 4. Item 4. The composition according to any one of Items 1 to 3, wherein an olive leaf extract is used as the oleuropein.
Item 5. Item 5. The composition according to any one of Items 1 to 4, wherein the composition is a food composition or a pharmaceutical composition.
Item 6. Item 6. The food composition according to Item 5, wherein the food composition is a health food, a functional food, a dietary supplement, a supplement, a food for specified health use, a food for a sick person, a combination food for a sick person, or a food for the elderly Composition.

  By ingesting the composition of the present invention as a pharmaceutical composition or a food composition, the amount of activity can be increased and anxiety can be reduced. For example, in an elderly person, when the amount of activity decreases and becomes confined to the house, aging is promoted, but the composition of the present invention improves positiveness and vitality, and promotes spontaneous motor activity, so it has good physical function. Can have a significant impact.

An outline of the open field test is shown. (a) Mouse body weight and food intake are shown. (b) Comparison of the amount of food consumed between olive leaf extract-containing food and normal food. Walking trajectory in the open field test. (a) Normal food, (b) Food containing olive leaf extract. Comparison of indicators of anxiety behavior. (a) Number of lane markings crossed, (b) Number of freezing actions, (c) Number of central area intrusions, (d) Time required to enter the central area

  In the present specification, the action of increasing activity and improving anxiety can be evaluated by an open field test for analyzing spontaneous movement (FIG. 1). Specifically, a candidate substance is mixed with food and taken by mammals (rats, mice, etc., particularly mice), one mammal is housed in one cage, and a walking track is recorded by a video tracking system or the like. . The trajectory of walking is less (shorter) when the anxious mental state and motivation for action are reduced, but it becomes more active and behavioral, and the walking trajectory is more (longer) when mental activity is active with less anxiety. .

  In the present invention, oleuropein is used to increase activity and improve anxiety.

  Oleuropain is contained in a large amount in olives, and an olive extract can be used. In particular, olive leaf extract is preferable because it contains oleuropein as a main component.

  In this specification, an olive means the olive (Olea europaea Linne) which is a plant of the Oleaceae family and the genus of olive (Olea). When extracting olive leaves, stems, branches, branches, etc. may be included along with the leaves. Other congeners such as O. welwitschii and O. paniculata can also be used as a raw material for olive leaf extraction.

  The plant is dried or pulverized as it is or if necessary, and then extracted with a solvent. Examples of the extraction solvent include water, lower alcohols (eg, methanol, absolute ethanol, hydrous ethanol, isopropanol, butanol, etc.), glycols (eg, ethylene glycol, propylene glycol, 1,3-butylene glycol, etc.), glycerin, acetone, Ketones such as methyl ethyl ketone and methyl isobutyl ketone, and organic solvents such as diethyl ether, diisopropyl ether, dioxane, tetrahydrofuran, N-methylpyrrolidone, acetonitrile, and ethyl acetate can be used alone or in combination of two or more.

  When an olive leaf extract is used as the oleuropein, it may be dried to a powder form, and can be optionally concentrated, diluted, mixed with other components, or the like.

  Extraction is usually carried out in the temperature range from normal temperature to the boiling point of the solvent under normal pressure, and after extraction, the extract is in the form of a solution, paste, gel, or powder by purification by filtration, evaporation of the solvent, column, etc. It can be. The extraction liquid can be subjected to purification treatment such as deodorization and decolorization.

  The oleuropein of the present invention can be used in a composition such as a food (including beverage) composition, a pharmaceutical composition or a supplement for the purpose of increasing the amount of activity or improving anxiety.

  In the present specification, examples of the neuropsychiatric disorder include diseases that easily cause dementia, Parkinson's disease, depression, depression, or depression such as diabetes and hyperthyroidism. Although aging is mainly used for aging, aging is also included when physical strength is reduced after surgery or long-term hospitalization.

  The composition of the present invention is in any dosage form, such as tablets, chewable tablets, troches, solutions, drinks, capsules, powders (powder), granules, fine granules, emulsions and suspensions. Also good. Pharmaceutical carriers, diluents or excipients included in these dosage forms include lactose, glucose, mannitol, dextrin, cyclodextrin, starch, sucrose, magnesium aluminate metasilicate, synthetic aluminum silicate, sodium carboxymethylcellulose, hydroxy Propyl starch, carboxymethylcellulose calcium, ion exchange resin, methylcellulose, gelatin, gum arabic, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinylpyrrolidone, polyvinyl alcohol, light anhydrous silicic acid, magnesium stearate, talc, tragacanth, bentonite, beegum, oxidation Titanium, sorbitan fatty acid ester, sodium lauryl sulfate, glycerin, fatty acid glycerin ester, purified lanolin, Riserozerachin, polysorbate, macrogol, vegetable oils, waxes, liquid paraffin, white petrolatum, fluorocarbons, nonionic surfactants, propylene glycol, water and the like.

  In the composition of the present invention, oleuropein is usually contained in an amount of 0.001% by mass or more, preferably about 0.01 to 20% by mass as a solid content.

  The composition of the present invention is preferably used to increase the activity amount or reduce or eliminate anxiety in mammals such as humans, monkeys, chimpanzees, cows, horses, pigs, dogs, cats, rabbits, goats, rats, mice, etc. It is valid. In mammals (especially humans), reducing or eliminating anxiety leads to increased activity. The daily intake of oleuropein for showing such action is about 0.01 mg to 10 g, preferably about 0.1 mg to 1 g, more preferably about 1 mg to 500 mg. It is preferable to take it about 4 times, but it may be taken once every 2 days, once every 3 days, once every 4 days, etc.

  When the composition of the present invention is a beverage, carbonated beverages, fruit juice beverages, dairy beverages, lactic acid bacteria beverages, tea beverages, alcoholic beverages, functional food material-containing beverages and the like can be mentioned.

  When the composition of the present invention is a food composition, specific examples include beverages (soft drinks (coffee, cocoa, juice, mineral drinks, tea drinks, green tea, tea, oolong tea, etc.), milk drinks, lactic acid bacteria drinks, yogurt Beverages, carbonated drinks, alcoholic beverages (Japanese liquor, western liquor, fruit liquor, etc.), spreads (custard cream, peanut cream, chocolate cream etc.), pastes (fruit paste, vegetable paste, sesame paste etc.), Western confectionery (chocolate, donuts, Pies, cream puffs, gums, gummies, jelly, candy, cookies, crackers, biscuits, snacks, cakes, puddings, etc., ice confections (ice cream, ice candy, sorbet, shaved ice, etc.), retort foods (curry, miscellaneous dishes, rice porridge, meat sauce) , Demiglace sauce, meatballs, han Burgers, oden seeds, red rice, etc.), instant foods (instant ramen, instant yakisoba, instant spaghetti, hot cake mix, etc.), jelly-like foods (jelly, agar, jelly-like beverages, etc.), dairy products (milk, cheese, yogurt) , Fresh cream, etc.), processed fruit (jam, marmalade, dried fruit, etc.). The food composition of the present invention includes so-called health foods, functional foods, dietary supplements, supplements, foods for specified health use, foods for the sick / combined foods for the sick, foods for the elderly, and the like.

  Next, the present invention will be described more specifically with reference to examples. However, the following examples do not limit the scope of the present invention.

Example 1
Five-week-old TSOD (Tsumura Suzuki Obese Diabetes) mice are divided into two groups, one with a normal diet (CE-2, Nippon Claire Co., Ltd.) and the other with olive leaf extract (trade names: Opiace, Eisai) Food Chemical Co., Ltd. (Oleuropain content 35% by mass or more) containing food (0.2% Opiace + CE-2) was given, and each group was individually raised to 37 weeks of age (9 months old). Mice are in a room with constant temperature, (23 ± 2 ℃), constant humidity (50 ± 10%), light / dark cycle 12 hours (light period 8: 00-20: 00), under conditions of free food and water Raised. As shown in FIGS. 2a and b, the weight and food intake of each individual were measured weekly, and the difference between the two groups was analyzed statistically. As a result, there was no significant difference in body weight throughout the age of all weeks (Fig. 2a), and food consumption increased transiently in the opiate-containing mice (Fig. 2b), but the cumulative food consumption was different between the two groups. None (Figure 2c). From the above results, it was suggested that opiace did not affect the amount of food and that opiace did not induce obesity.

Example 2
As an analysis method of spontaneous motor ability and anxiety-like behavior, an open field test was conducted to analyze spontaneous behavior of mice in a new environment. In the open field test, a mouse is placed in a new, wide and bright environment and freely explored for a certain period of time. Mice have the property of favoring the walls, and this tendency becomes stronger in timid mice. Therefore, the higher the degree of searching for the central part, the more active and active, the lower the level of anxiety. From 30 minutes before the start of the open field test, the mouse was moved to the laboratory and acclimatized. Prepare a plastic box (size: width 35 x depth 35 x height 20cm) on a personal computer using a web camera and behavior test analysis software ANY-maze vide tracking system (Brain Science Idea, Inc.) A 15 × 15 cm square (center area) and a line that divides the field into four sections were drawn in the center to set the area. A habituated mouse was placed in the corner of the field and a 10-minute action was recorded in the video. Figures 3a and b show the walking trajectory of the mouse in a representative field. It was revealed that mice fed the opiate-containing diet showed higher activity and more invaded the central area than mice fed the normal diet (Fig. 3a, b).

Example 3
Next, in order to analyze the effects of opiace on spontaneous motor ability and anxiety-like behavior, from the video data taken, the number of crossings of the division line (Fig. 4a) and freezing behavior as an indicator of anxiety-like behavior as an indicator of spontaneous motor ability The number of times (Fig. 4b), the number of intrusions into the central area (Fig. 4c), and the time taken to enter the central area (Fig. 4d) are digitized using the behavioral test analysis software ANY-maze vide tracking system, and t-tests are performed as statistical processing It was. As a result of comparison with normal diet mice, it was found that mice fed opiate-containing diets had a high number of crossings and high activity (Fig. 4a). In addition, the opiace-containing diet reduced the number of freezing actions (Figure 4b), increased the number of central area intrusions (Figure 4c), and reduced the time required to enter the central area (Figure 4d). From the above results, it was clarified that the activity reduction and anxiety-like behavior of TSOD mice were suppressed by feeding opiace-containing food, suggesting that olive leaf extract has increased activity and anxiolytic action. It was.

Claims (5)

A composition for improving activity reduction and anxiety for promoting motor activity by activating mental activity, comprising an olive leaf extract containing oleuropein as a main component as an active ingredient. The composition according to claim 1, wherein the cause of decreased activity and anxiety is a decrease in mental function due to aging. The composition according to claim 1, wherein the cause of decreased activity and anxiety is a neuropsychiatric disorder. The composition according to any one of claims 1 to 3, wherein the composition is a food composition or a pharmaceutical composition. 5. The food composition according to claim 4, wherein the food composition is a health food, a functional food, a dietary supplement, a supplement, a food for specified health use, a food for a sick person, a combination food for a sick person, or a food for an elderly person. Composition.