JP6484975B2 - Glycerophospholipid-containing powder and health food - Google Patents

Description

  The present invention relates to a powder containing glycerophospholipid and a health food obtained by using the powder.

  Glycerophospholipid is a phospholipid having a glycerol skeleton, and has attracted attention as a physiologically active substance that plays various roles in vivo. As its physiological activity, for example, an action of improving lipid metabolism (Patent Document 1) and prevention / improvement of liver damage (Patent Document 2) have been reported. Particularly in recent years, phosphatidylserine has been found to have a brain function improving effect and is considered promising as a food material.

  Various physiologically active substances are marketed as health foods in various forms such as drinks and soft capsules. Among these, the product form using powder raw materials such as hard capsules, tablets, and granule products occupies most of the market. Among the physiologically active substances, glycerophospholipid has very specific properties. As is apparent from the fact that it is a component constituting a biological membrane, it is an amphiphilic substance, and therefore has high adhesiveness when dried and powdered. For this reason, commercially available powder products containing glycerophospholipids often have poor fluidity and powder dispersibility due to stickiness, which often hinders commercialization. For example, in the case of hard capsules and granule products, due to poor flowability, it can not be blended in large quantities considering the filling properties, and in the case of tablet products, stickiness increases as the blending amount increases, and tableting is not possible There is. In either case, the amount of the glycerophospholipid-containing powder in the product must be reduced, and in order to consume the recommended amount, the product must be designed to consume a large amount. In the case of tablets, there is a problem that even if the amount of the glycerophospholipid-containing powder is small, uneven distribution occurs due to poor powder dispersibility in the blended raw material, and the tablet surface becomes patchy and the appearance deteriorates. is there. In addition, commercially available powder products have another problem that the powder solidifies when it absorbs moisture during storage.

Although solutions have been made to the specific properties of such glycerophospholipids, no effective solution has yet been found. For example, Patent Document 3 discloses a technique for improving the dispersibility of phosphatidylserine by using gelatin in combination with a sucrose fatty acid ester. Although this technique improves dispersibility in water, The properties of the glycerophospholipid itself do not change, and it does not lead to any improvement in the uniform dispersibility in the powder raw material. Patent Document 4 discloses a technique that consists only of sodium ascorbate and saccharides and improves fluidity and tablet formability, but this technique cannot improve the stickiness of glycerophospholipids.
Thus, it is desired to reduce the stickiness of glycerophospholipid-containing powders, improve fluidity and powder dispersibility, and further improve versatility as a health food ingredient by preventing caking during storage. Yes.

JP 2004-337177 A Japanese Patent Laid-Open No. 10-84879 JP 2013-143913 A JP 2010-248108 A

  An object of the present invention is to provide a glycerophospholipid-containing powder that suppresses stickiness and has improved fluidity and powder dispersibility as a powder material for health foods. Another object is to provide a health food in which glycerophospholipids are uniformly dispersed.

As a result of intensive research in order to solve the above problems, the present inventors have included glycerophospholipid, insoluble dietary fiber and saccharide in a specific blending amount to obtain a powder having a specific particle size range, The present inventors have found the knowledge of solving the above problems and have completed the present invention.
That is, the present invention includes the following (1) to (3).
(1) The following component (A) Glycerophospholipid 10-70 mass%
(B1) 5-45% by mass of insoluble dietary fiber
(B2) Carbohydrate 5 to 45 mass%
And a glycerophospholipid-containing powder having an average particle size of 10 to 1000 μm.
(2) Furthermore,
(C) Anti-caking agent 0.01-2.0 mass%
The glycerophospholipid-containing powder according to (1) above, comprising
(3) A glycerophospholipid-containing health food using the glycerophospholipid-containing powder according to (1) or (2).

  The present invention can provide a glycerophospholipid-containing powder that reduces stickiness, which is a property of glycerophospholipid, improves fluidity and powder dispersibility as a powder raw material, and suppresses caking during storage. Furthermore, by using this as a raw material for powders of health foods such as hard capsules, tablets and granules, it is possible to increase the amount of glycerophospholipid in the product and eliminate spots on the tablet surface. A product can be offered.

It is a photograph of the tablet manufactured in Example 2-3 (left figure) and the tablet produced in Comparative Example 2-2 (right figure).

Hereinafter, the present invention will be described in more detail.
(Glycerophospholipid-containing powder)
The glycerophospholipid-containing powder of the present invention is a powder containing glycerophospholipid (A), insoluble dietary fiber (B1), and carbohydrate (B2).

(Glycerophospholipid (A))
The glycerophospholipid used in the present invention refers to a compound having a structure in which glycerin has acyl groups derived from fatty acids at the 1- and 2-positions and a polar group containing phosphoric acid is bonded to the 3-position. Examples include phosphatidylcholine (PC), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidic acid (PA), and a mixture of two or more. PC, PS, PE, PA or a mixture of two or more thereof is preferred. The glycerophospholipid (A) of the present invention can be extracted and purified from animals and plants. Examples thereof include plants such as soybean, rice, corn, rapeseed, cottonseed, wheat, sunflower, safflower, and animals such as egg yolk and seafood. Or it can also obtain by a chemical reaction, an enzyme reaction, etc., and what is marketed as a reagent or a raw material can also be used.

  The blending amount of glycerophospholipid (A) with respect to the glycerophospholipid-containing powder is preferably 10 to 70% by mass, more preferably 15 to 60% by mass. When the glycerophospholipid is less than 10% by mass, the active ingredient per one when blended into a tablet or hard capsule is reduced, the number of ingested grains must be increased, which is a heavy burden for consumers. Moreover, when glycerophospholipid exceeds 70 mass%, stickiness of glycerophospholipid and suppression of caking cannot be suppressed, and fluidity | liquidity and powder dispersibility cannot be improved.

(Insoluble dietary fiber (B1))
Dietary fiber is a general term for carbohydrates that are not digested or absorbed in the human digestive tract. Dietary fiber is roughly classified into water-soluble dietary fiber and insoluble dietary fiber. The dietary fiber used in the present invention is insoluble dietary fiber (B1). Examples include cellulose, hemicellulose, lignan, chitin, etc. Among these, cellulose is more preferable. The insoluble dietary fiber (B1) of the present invention is extracted and purified from animals and plants, and purified from, for example, apples, corn, beets, soybeans, wheat, wood (pulp), shellfish shells, and the like. Alternatively, it can be obtained by a chemical reaction, or a commercially available reagent or raw material can be used.
In addition, the amount of insoluble dietary fiber can be measured according to the enzyme-weight method (modified Prosky method) used in the method for measuring insoluble dietary fiber in the 5th edition supplementary food standard ingredient table (Ministry of Education, Culture, Sports, Science and Technology). it can.

  Since insoluble dietary fiber (B1) has oil-absorbing property, stickiness can be suppressed by mix | blending with the powder containing glycerophospholipid. However, since the insoluble dietary fiber (B1) itself has poor fluidity, if the blending amount is too large, it causes problems such as poor fluidity. The blending amount of the insoluble dietary fiber (B1) with respect to the glycerophospholipid-containing powder is preferably 5 to 45% by mass. When the insoluble dietary fiber (B1) is less than 5% by mass, stickiness of glycerophospholipid cannot be suppressed. Moreover, when insoluble dietary fiber (B1) exceeds 45 mass%, since fluidity | liquidity will worsen, powder dispersibility cannot be improved.

(Sugar (B2))
Carbohydrate is a general term for compounds having a monosaccharide as a constituent component. Examples of the saccharide (B2) used in the present invention include monosaccharides such as glucose, fructose and galactose, disaccharides such as lactose, sucrose and maltose, polysaccharides such as starch and glycogen, xylitol, mannitol and reduced isomalt Examples thereof include sugar alcohols such as loin. Of these, sugar alcohols are more preferred. Examples of the saccharide (B2) of the present invention include extraction and purification from animals and plants, such as sugar cane, sugar beet, potato, tapioca, rice, wheat, corn and the like. Alternatively, it can be obtained by a chemical reaction or the like, or a commercially available reagent or raw material can be used.

  Since the saccharide (B2) has high fluidity, the fluidity and powder dispersibility of the powder containing glycerophospholipid can be improved. The blending amount of the saccharide (B2) with respect to the glycerophospholipid-containing powder of the present invention is preferably 5 to 45% by mass. When the saccharide (B2) is less than 5% by mass, the fluidity cannot be improved. On the other hand, it is preferable to adjust the sugar (B2) to 45% by mass or less because the amount of glycerophospholipid added can be increased.

(Anti-caking agent (C))
By using the anti-caking agent (C), caking during storage of the powder containing glycerophospholipid can be prevented. Examples include magnesium stearate, calcium stearate, fine silicon dioxide, microcrystalline cellulose, sucrose fatty acid ester, glycerin fatty acid ester, calcium carbonate, calcium phosphate and the like. Of these, fine silicon dioxide is preferred.

  The blending amount of the anti-caking agent (C) with respect to the glycerophospholipid-containing powder of the present invention is preferably 0.01 to 2.0% by mass. When the anti-caking agent (C) is less than 0.01% by mass, glycerophospholipids are consolidated during storage, resulting in coarse particles. On the other hand, when the amount exceeds 2.0% by mass, the adverse effect that the amount of glycerophospholipid and the like decreases, the upper limit of the amount is preferably 2.0% by mass or less.

(Other ingredients)
In addition to glycerophospholipid (A), insoluble dietary fiber (B1), and carbohydrate (B2), the glycerophospholipid-containing powder of the present invention includes fats and oils, proteins, water-soluble dietary fibers, minerals such as minerals, vitamins, etc. You may mix | blend a functional material etc.

(Glycerophospholipid-containing powder)
The glycerophospholipid-containing powder of the present invention is a powder containing (A) 10 to 70% by mass of glycerophospholipid, (B1) 5 to 45% by mass of insoluble dietary fiber, and (B2) 5 to 45% by mass of carbohydrate. is there. As a manufacturing method, an ordinary powdering method can be applied. For example, glycerophospholipid (A), insoluble dietary fiber (B1), and carbohydrate (B2) are insoluble in a method of pulverizing after mixing at a specific blending amount, or in a reaction solution of glycerophospholipid obtained by extraction or enzymatic reaction. Examples thereof include a method in which a dietary fiber excipient (B1) and a saccharide (B2) added and mixed are dried and then pulverized. Examples of the drying method include spray drying, hot air drying, drum drying, freeze drying, fluidized bed drying, and continuous vacuum drying. Examples of the grinding method after drying include a grinding method using a cutting mill, a hammer mill, an impact mill, an airflow mill, and the like. Moreover, (C) 0.01-2.0 mass% anti-caking agent can be added after grinding | pulverization.
The average particle size of the glycerophospholipid-containing powder of the present invention is 10 to 1000 μm. The average particle diameter is measured with a dry laser diffraction particle size distribution analyzer. When the average particle diameter exceeds 1000 μm, for example, a tableting failure such as capping in which the tablet peels into a cap shape is likely to occur, and the tablet moldability is deteriorated. When the average particle diameter is less than 10 μm, for example, when powder raw materials are mixed, a classification phenomenon occurs naturally, and the uniformity of the content is impaired. In the present invention, the average particle diameter means a volume-based average particle diameter measured using a dry laser diffraction particle size distribution analyzer.

(healthy food)
The glycerophospholipid-containing powder of the present invention can be made into various forms of health foods so as to facilitate continuous ingestion by taking advantage of its excellent characteristics. Specifically, it can be prepared, for example, as a tablet, granule, or hard capsule.

(Health food manufacturing method)
The method for producing a glycerophospholipid-containing health food is characterized by using the glycerophospholipid-containing powder of the present invention and can be produced using a known existing production apparatus.
The method for producing a glycerophospholipid-containing tablet includes a step of mixing the glycerophospholipid-containing powder of the present invention, an excipient and a lubricant to obtain a mixture, and a step of tableting the mixture with a tableting machine. In the step of obtaining a mixture, other active ingredients may be mixed. By using the glycerophospholipid-containing powder of the present invention, it is possible to increase the glycerophospholipid content per grain and to eliminate the uneven pattern on the tablet surface.

  The method for producing granules and hard capsules includes a step of filling the glycerophospholipid-containing powder of the present invention into a packaging bag such as a three-sided seal or a stick or a hard capsule. Moreover, you may provide the process of mixing powder, such as an excipient | filler and another active ingredient, and obtaining a mixture before the process of filling. By using the glycerophospholipid-containing powder of the present invention, the fluidity is improved as compared with the conventional glycerophospholipid powder, so that the filling property is improved, and the amount of glycerophospholipid blended per bag or grain can be increased.

The present invention will be specifically described below with reference to examples.
First, the evaluation method in each example is shown.
1. 1. Evaluation method of glycerophospholipid-containing powder 1-1. Measurement method of average particle diameter Volume standard average particle diameter was measured by a dry method using a laser diffraction particle size distribution analyzer (SALD-2100: manufactured by Shimadzu Corporation).
1-2. Measuring method of fluidity The angle of repose of the powder was measured as a method for evaluating the fluidity of the powder. The smaller the angle of repose, the better the fluidity. The angle of repose was measured according to the method specified in the “15th revision, Japanese Pharmacopoeia Manual”.
1-3. Evaluation Method of Stickiness 10 g of glycerophospholipid-containing powder was weighed into a mortar, and pestle was ground 10 times. The monitor was touched by 10 monitors, and the strength of stickiness was evaluated according to the following criteria.
○: No stickiness.
Δ: Feels somewhat sticky.
X: It is sticky.
1-4. Evaluation method of caking property The ratio of the solidified powder after moisture absorption is regarded as caking property, and the measurement method is described below. 10 g of glycerophospholipid-containing powder was weighed into a petri dish, and absorbed in an open-temperature system for 1 hour in a constant temperature and humidity chamber with a humidity of 75% and 40 ° C. After measuring the mass of the moisture-absorbed powder, the powder was vibrated 100 times using a 20-mesh sieve, and the mass of the portion that was consolidated and remained on the sieve was measured. The formula for the caking property (%) is shown below. If it is not consolidated, it easily passes through the sieve, so the consolidation (%) is low.
“Consolidation property (%) = mass of glycerophospholipid-containing powder on sieve (g) / mass of glycerophospholipid powder before sieve (g) × 100”
1-5. Method for measuring glycerophospholipid content The glycerophospholipid content of the glycerophospholipid-containing powder was measured by high performance liquid chromatography. High performance liquid chromatography conditions are described below.
Model: High-performance liquid chromatography (Hitachi, Ltd. Model D7000)
Column; silica gel column (straight diameter 4.6 mm x length 250 mm)
Mobile phase; mixed solvent detection of acetonitrile: methanol: phosphoric acid = 775: 35: 9 (volume ratio); ultraviolet wavelength 202 nm

2. Tablet Evaluation Method The glycerophospholipid content and the appearance of the tablet were evaluated when glycerophospholipid-containing powder was blended to the limit that did not cause tableting problems. Tablets were tableted using a rotary rotary tableting machine (“PICCOLA” manufactured by STEC Co., Ltd.) with a mortar and pestle of 8.0 mm and R6.0 at a mass of 270 mg and a tableting pressure of 5 to 8 kN. .
2-1. Method for measuring glycerophospholipid content Tablets were pulverized, and the obtained powder was measured for glycerophospholipid content by the measurement method described in 1-5 above.
2-2. Appearance The appearance of the tablets was evaluated according to the following criteria.
○: Spotted pattern is not recognized.
(Triangle | delta): A spot pattern is recognized in part.
X: A spotted pattern is recognized as a whole.

Details of the three glycerophospholipids used in the examples and comparative examples of the present invention are as follows.
・ Ultra REC P: Commercial product “Ultra REC P” (trade name) manufactured by ADM Japan Co., Ltd.
Enzyme transfer PS792: Glycerophospholipid with a PS content of 60% obtained by carrying out a phosphatidyl group transfer reaction of PC using the method described in JP-A-63-36792. PS70PN: BN Co., Ltd. (sales company) Commercial product "LIPAMINE-PS70PN" (trade name)
Table 1 shows the component contents of each glycerophospholipid.
Details of other materials are as follows.
・ Cellulose: Insoluble dietary fiber, “Theolas ST-100” (trade name) manufactured by Asahi Kasei Chemicals Corporation
・ Reduced isomaltulose: Carbohydrate, “Powdered Paratinite PNP” (trade name) manufactured by Mitsui Sugar Co., Ltd.
-Fine silicon dioxide: anti-caking agent, "Silopage # 720" (trade name) manufactured by Fuji Silysia Co., Ltd.

(Production of powder)
In Examples 1-1 to 1-6 and Comparative Examples 1-1 to 1-5, powders were produced by the following method. Insoluble dietary fiber, sugar and water were added to a predetermined amount of glycerophospholipid, mixed and then dried by a continuous vacuum drying method. The obtained dried product was pulverized using a power mill (manufactured by Dalton Co., Ltd .: Model P-55) to obtain a glycerophospholipid-containing powder. The anti-caking agent was added to and mixed with the glycerophospholipid-containing powder obtained by grinding.
Tables 2 and 3 show the evaluation results of the average particle size, fluidity, stickiness, caking property, and glycerophospholipid content of the obtained glycerophospholipid-containing powder, together with the types and amounts of the blending components in each Example and Comparative Example. Indicates.
In addition, about each Example and the comparative example, the name of P1-P6 and Q1-Q5 was attached | subjected to the obtained glycerophospholipid containing powder.

(Tablet adjustment)
In Examples 2-1 to 2-6 and Comparative Examples 2-1 to 2-5, glycerophospholipid-containing powders P1 to P1 produced in Examples 1-1 to 1-6 and Comparative Examples 1-1 to 1-5 Using P6, Q1 to Q5, tablets were produced by the following method. A predetermined amount of glycerophospholipid-containing powder, an excipient and a lubricant were mixed, and then tableted using a rotary rotary tableting machine (“PICCOLA” manufactured by ESTEC Co., Ltd.). Tableting conditions were 8.0 mm, R6.0 mortar and punch, mass 270 mg / grain, turret rotation speed 15 rpm, tableting pressure 5-8 kN.
Tables 4 and 5 show the results of evaluation of the glycerophospholipid content in the tablet and the appearance of the tablet, together with the types and amounts of the ingredients in each Example and Comparative Example. In Tables 4 and 5, the types of the glycerophospholipid-containing powders used are shown using the names described in Tables 2 and 3. Details of other materials are as follows.
・ Maltodextrin: excipient, “Paindex # 2” (trade name) manufactured by Matsutani Chemical Co., Ltd.
・ Calcium stearate… Lubricant, Taihei Chemical Industry Co., Ltd. “Calcium stearate (vegetable)” (trade name)

Regarding the evaluation results of the glycerophospholipid-containing powders in Tables 2 and 3, in Examples 1-1 to 1-6, stickiness is suppressed, the angle of repose is as small as 35 to 40 °, and the fluidity is good. A contained powder was obtained. Further, in Examples 1-1 to 1-4, the caking property is 21 to 29%, and further, Examples 1-5 to 1-6 to which fine silicon dioxide is added are 5% or less, and the caking property is improved. It is clear that
On the other hand, in Comparative Examples 1-1 to 1-5, since the insoluble dietary fiber (B1) or the saccharide (B2) used in the present invention is not contained, stickiness cannot be improved and the angle of repose is 50 °. From the above, it has been clarified that a glycerophospholipid-containing powder that is practically used cannot be obtained. Further, the caking properties of Comparative Examples 1-1 to 1-2 were 63 to 68%, and Comparative Examples 1-3 to 1-5 to which fine silicon dioxide was added were 49 to 53%, which were considerably consolidated. .

About the evaluation result of the tablet of Table 4 and Table 5, in Example 2-1 to 2-6 tableted using the glycerophospholipid containing powder of this invention, it is 21.0-22. 5 mass% could be mix | blended. In addition, the tablet surfaces of the examples were in a clean state with glycerophospholipids dispersed uniformly. As an example of a tablet having a good appearance, the tablet of Example 2-3 is shown in FIG.
On the other hand, in Comparative Examples 2-1 to 2-5 using the glycerophospholipid-containing powder that does not contain insoluble dietary fiber or carbohydrate, tableting occurs when the glycerophospholipid is blended at a high concentration because of poor stickiness and fluidity. A failure has occurred. Therefore, only 10.5% of glycerophospholipid can be blended into the tablet. Further, since the powders of Comparative Examples 1-1 to 1-5 have poor powder dispersibility, glycerophospholipid is unevenly distributed on the tablet surface, and the appearance is remarkably impaired. As an example of the tablet in which glycerophospholipid is unevenly distributed, the tablet of Comparative Example 2-2 is shown in FIG.

Claims (3)

Following components (A) glycerophospholipids 1 5-70 wt%
(B1) 5-45% by mass of insoluble dietary fiber
(B2) Carbohydrate 5 to 45 mass%
(B1) A glycerophospholipid-containing powder having an insoluble dietary fiber of cellulose, (B2) a carbohydrate of reduced isomaltulose, and an average particle size of 10 to 1000 μm. further,
(C) Anti-caking agent 0.01-2.0 mass%
The glycerophospholipid-containing powder according to claim 1, comprising: The manufacturing method of the glycerophospholipid containing health food using the glycerophospholipid containing powder of Claim 1 or Claim 2.