JP6437725B2 - E. coli growth inhibitor, pet toilet sand using the same, and E. coli growth suppression method - Google Patents
E. coli growth inhibitor, pet toilet sand using the same, and E. coli growth suppression method Download PDFInfo
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- 241000588724 Escherichia coli Species 0.000 title claims description 57
- 239000003966 growth inhibitor Substances 0.000 title claims description 19
- 238000000034 method Methods 0.000 title claims description 15
- 206010053759 Growth retardation Diseases 0.000 title claims description 3
- 239000004576 sand Substances 0.000 title description 4
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims description 48
- 229910004298 SiO 2 Inorganic materials 0.000 claims description 6
- 230000002401 inhibitory effect Effects 0.000 claims description 6
- 229910018072 Al 2 O 3 Inorganic materials 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 2
- 239000002609 medium Substances 0.000 description 22
- 230000001717 pathogenic effect Effects 0.000 description 17
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 238000005259 measurement Methods 0.000 description 10
- 229920001817 Agar Polymers 0.000 description 9
- 239000008272 agar Substances 0.000 description 9
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 7
- 230000009422 growth inhibiting effect Effects 0.000 description 6
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241001333951 Escherichia coli O157 Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000005757 colony formation Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 235000013980 iron oxide Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- -1 6% by weight or more Chemical compound 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 239000011162 core material Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- VBMVTYDPPZVILR-UHFFFAOYSA-N iron(2+);oxygen(2-) Chemical class [O-2].[Fe+2] VBMVTYDPPZVILR-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000013630 prepared media Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
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- Housing For Livestock And Birds (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
本発明は、大腸菌増殖抑制剤、これを用いたペット用トイレ砂、及び、大腸菌増殖抑制方法に関する。 The present invention relates to an E. coli growth inhibitor, a pet litter using the same, and a method for inhibiting E. coli growth.
大腸菌とは、哺乳類等の温血動物の腸管内にも生息する細菌であり、大腸菌の多くは無害である。しかしながら、その中には人の体内に取り込まれると体内で毒素を産生し、強い腹痛や下痢をもたらし人を重篤な症状に陥れる病原性大腸菌もある。 E. coli is a bacterium that also inhabits the intestinal tract of warm-blooded animals such as mammals, and many of E. coli are harmless. However, some pathogenic E. coli cells, when taken into the human body, produce toxins in the body that can cause severe abdominal pain and diarrhea, resulting in serious symptoms.
病原性大腸菌を体内に取り込まないようにするためには、病原性大腸菌が付着した物体を仮に触ったとしても体内に取り込む前に除去するべく、適時手洗いを行う等の方法が効果的であるが、仮に物体に病原性大腸菌が付着した場合でも、その増殖を抑えることは非常に重要である。 In order to prevent pathogenic Escherichia coli from being taken into the body, a method such as hand washing at an appropriate time is effective in order to remove the pathogenic E. coli adhering object before taking it into the body even if it is touched. Even if pathogenic E. coli adheres to an object, it is very important to suppress its growth.
病原性大腸菌の増殖を抑える公知技術として、例えば、下記特許文献１に、食塩及び酢酸を加えた殺菌用組成物を用い、食品調理用器具を３０〜４５℃で接触させる技術が開示されている。 As a known technique for suppressing the growth of pathogenic Escherichia coli, for example, the following Patent Document 1 discloses a technique in which a food cooking utensil is contacted at 30 to 45 ° C. using a sterilizing composition to which salt and acetic acid are added. .
しかしながら、上記特許文献１に記載の技術は液体であるため濡らすことを避けたい物品には用いにくいといった課題、所定の温度で対象物に接触させなければならないといった課題もある。すなわちより簡便で使いやすい病原性大腸菌の増殖を抑制する方法が望まれる。 However, since the technique described in Patent Document 1 is a liquid, there is a problem that it is difficult to use for an article that it is desired to avoid wetting, and there is a problem that it must be brought into contact with an object at a predetermined temperature. That is, a simpler and easier-to-use method for suppressing the growth of pathogenic E. coli is desired.
そこで本発明は上記課題に鑑み、より簡便で使いやすい大腸菌増殖抑制剤及び大腸菌増殖抑制方法を提供することを目的とする。 In view of the above problems, an object of the present invention is to provide an E. coli growth inhibitor and a method for suppressing E. coli growth that are simpler and easier to use.
上記課題について本発明者らが鋭意検討を行ったところ、いわゆるリモナイトが大腸菌の増殖抑制剤を有することを発見し、本発明を完成させるに至った。 When the present inventors diligently studied about the said subject, it discovered that what is called limonite has the growth inhibitor of Escherichia coli, and came to complete this invention.
すなわち、上記課題を解決する一の観点に係る大腸菌増殖抑制剤は、リモナイトを含むことを特徴とする。 That is, the Escherichia coli growth inhibitor according to one aspect of solving the above-mentioned problem is characterized by containing limonite.
また、本発明の他の一観点に係るペット用トイレ砂は、リモナイトを含む大腸菌増殖抑制剤を含むことを特徴とする。 In addition, pet toilet sand according to another aspect of the present invention includes an E. coli growth inhibitor containing limonite.
また、本発明の他の一観点に係る大腸菌増殖抑制方法は、被処理物体にリモナイトを添加し、混合することを特徴とする。 Moreover, the E. coli growth inhibition method according to another aspect of the present invention is characterized in that limonite is added to an object to be treated and mixed.
以上、本発明によって、簡便な大腸菌増殖抑制剤、これを用いた大腸菌抑制方法及びペット用トイレ砂を提供することを目的とする。 As mentioned above, it is an object of the present invention to provide a simple E. coli growth inhibitor, an E. coli suppression method using the same, and pet litter.
以下、本発明の実施形態について図面を用いて詳細に説明する。ただし、本発明は多くの異なる形態による実施が可能であり、以下に示す実施形態、実施例の例示にのみ限定されるわけではない。 Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. However, the present invention can be implemented in many different forms, and is not limited only to the embodiments and examples described below.
本実施形態に係る大腸菌増殖抑制剤は、リモナイトを含むことを特徴とする。 The Escherichia coli growth inhibitor according to this embodiment is characterized by containing limonite.
本実施形態において、大腸菌は病原性大腸菌であることでその効果が明確になる。具体的には、病原性大腸菌の増殖を抑制することで、病原性大腸菌が人体内に取り込まれてしまうリスクを大きく低下させることが可能となる。ここで限定されるわけではないが、増殖抑制対象となる病原性大腸菌の具体的な例としては、Ｏ１５７、Ｏ１３８等を例示することができる。 In this embodiment, E. coli is a pathogenic E. coli, and its effect becomes clear. Specifically, by suppressing the growth of pathogenic E. coli, the risk of pathogenic E. coli being taken into the human body can be greatly reduced. Although it is not necessarily limited here, O157, O138, etc. can be illustrated as a specific example of pathogenic Escherichia coli used as the object of growth suppression.
また、本大腸菌増殖抑制剤において用いられる「リモナイト」とは、鉄の酸化物を主成分とする鉱物であって、日本においては熊本県の阿蘇山周辺において多量に採取可能である「阿蘇リモナイト」であることが顕著な効果を達成する観点から好ましい。リモナイトの成分については、鉄以外にも天然ミネラル等不確定な物質も含まれているため完全な成分分析は困難であるものの、本大腸菌増殖抑制剤の効果を発揮することができると考えられる配合は、阿蘇リモナイトの組成から求めたところ、主な組成はリモナイトの全量を１００重量部とした場合に、Ｆｅ酸化物６０重量部以上８０重量部以下、ＳｉＯ２を８重量部以上１８重量部以下、Ａｌ２Ｏ３を１重量部以上５重量部以下、ＣａＯを０．１重量部以上２重量部以下、及び残部が不可避の有機物及び無機物であることが好ましい。ここでＦｅ酸化物とは、ＦｅＯ（ＯＨ）及びＦｅ２Ｏ３の少なくともいずれかを含む鉄の酸化物をいう。なお限定されるわけではないが、リモナイトの全量が１００重量部であるとする場合において「部」は「％」と置き換えて考えてもよい。 In addition, “limonite” used in the present Escherichia coli growth inhibitor is a mineral mainly composed of iron oxides, and in Japan it can be collected in large quantities around Mt. Aso in Kumamoto Prefecture. It is preferable from the viewpoint of achieving a remarkable effect. The ingredients of limonite contain uncertain substances such as natural minerals in addition to iron, so complete component analysis is difficult, but it is thought to be able to exert the effects of the present Escherichia coli growth inhibitor. Calculated from the composition of Aso limonite, the main composition is 60 parts by weight or more and 80 parts by weight or less of Fe oxide and 8 parts by weight or more and 18 parts by weight or less of SiO 2 when the total amount of limonite is 100 parts by weight. Al 2 O 3 is preferably 1 to 5 parts by weight, CaO is 0.1 to 2 parts by weight, and the balance is unavoidable organic and inorganic. Here, the Fe oxide refers to an iron oxide containing at least one of FeO (OH) and Fe 2 O 3 . Although not limited, in the case where the total amount of limonite is 100 parts by weight, “part” may be replaced with “%”.
また、本大腸菌増殖抑制剤は、様々な用途に使用可能である。例えば、リモナイトを粉末状にすることで、物品に対してふりかけるだけで大腸菌の増殖抑制効果を得ることができる。またリモナイト自身に毒性はなく、体内に取り込まれても人体に害はないため、犬や猫等のペットにふりかけることができ、また、リモナイトをペット用のトイレ砂に用いることでも大腸菌増殖を抑制することができる。ペット用のトイレ砂としては、リモナイトをそのまま使用してもよく、砂の芯材にリモナイトをコーティングさせるようにしてもよい。なお、ペット用トイレ砂に用いる場合、限定されるわけではないが、ペット用トイレ砂全体の重量を１００重量部とした場合、リモナイトを１重量部以上２０重量部以下の範囲としておくことが好ましく、より好ましくは５重量部以上１０重量部以下である。１重量部以上としておくことで十分な効果を得ることができる一方、２０重量部以下とすることで過剰な量による重量増加を防ぐことができ、１０重量部以下とすることでこの効果が顕著となる。 In addition, the present Escherichia coli growth inhibitor can be used for various applications. For example, by making limonite into a powder form, the effect of suppressing the growth of E. coli can be obtained simply by sprinkling the product. Limonite itself is not toxic and can be sprinkled on pets such as dogs and cats because it is not harmful to the human body when it is taken into the body. can do. As toilet sand for pets, limonite may be used as it is, or the core material of sand may be coated with limonite. When used for pet litter, it is not limited, but when the total weight of litter for pet is 100 parts by weight, limonite is preferably in the range of 1 to 20 parts by weight. More preferably, it is 5 parts by weight or more and 10 parts by weight or less. A sufficient effect can be obtained by setting it to 1 part by weight or more, while an increase in weight due to an excessive amount can be prevented by setting it to 20 parts by weight or less, and this effect is remarkable by setting it to 10 parts by weight or less. It becomes.
上記の記載から明らかなように、本大腸菌増殖抑制剤は、被処理物体上にリモナイトを添加することで大腸菌増殖抑制を行うことができ、好ましくはさらに物体とリモナイトを混合することでさらに効果を得ることができる。なおこの場合、上記の例と同様、被処理物体全体及びリモナイトの合計重量を１００重量部とした場合に、リモナイトを１重量部以上含ませておくことが好ましい。 As is clear from the above description, the present E. coli growth inhibitor can suppress E. coli growth by adding limonite onto the object to be treated, and preferably further improves the effect by mixing the substance and limonite. Can be obtained. In this case, as in the above example, when the total weight of the entire object to be treated and limonite is 100 parts by weight, it is preferable to include 1 part by weight or more of limonite.
本大腸菌増殖抑制剤は、天然に豊富に存在するリモナイトを原料とするものであり、入手が容易であるとともに簡便に粉末状にすることができしかもその粒径の制御が容易である、また、粉末であるため濡らすことのできない物体に対しても使用することができる。更には、樹脂等のバインダーと混合してコーティング剤として被処理物体の周囲に安定的に固着して使用することができる。加えて、リモナイトは口に入っても害はないため、身の回りのものに安心して使用することができるといった効果もある。すなわち、本発明によって、より簡便で使いやすい大腸菌増殖抑制剤及び大腸菌増殖抑制方法を提供することができる。 The present Escherichia coli growth inhibitor is made from limonite that is abundant in nature, and is easily available and can be easily powdered, and its particle size can be easily controlled. It can also be used for objects that cannot be wetted because they are powders. Furthermore, it can be mixed with a binder such as a resin and stably used as a coating agent around the object to be treated. In addition, since limonite is not harmful even if it enters the mouth, it has the effect that it can be used with peace of mind. That is, the present invention can provide an E. coli growth inhibitor and a method for suppressing E. coli growth that are simpler and easier to use.
ここで、病原性大腸菌に対するリモナイトの大腸菌増殖抑制効果を確認した。以下具体的に説明する。 Here, the inhibitory effect of limonite on pathogenic E. coli was confirmed. This will be specifically described below.
（大腸菌育成試験：ＤＨ５α）
ＬＢ培地及び阿蘇リモナイトの合計重量を１００重量％とした場合に、阿蘇リモナイトを０重量％としたもの（ＬＢ培地のみのもの）、１重量％としたもの、３重量％としたもの、６重量％としたもの、１０重量％としたもの５種を作製し、非病原性大腸菌ＤＨ５αを培養した。具体的には、所定量の阿蘇リモナイトをＬＢ培地に添加した後、１２１℃１時間オートクレーブした後、非病原性大腸菌ＤＨ５αを定量播種し、１２１℃１時間オートクレーブした後、３７℃で１２時間静置しその結果を確認した。この結果を図１、図２に示す。図１は各軟寒天培地の写真であり、図２は、この写真において示される各シャーレにおけるコロニーの形成率をそれぞれ示すものである。
(E. coli breeding test: DH5α)
When the total weight of LB medium and Aso limonite is 100% by weight, Aso limonite is 0% by weight (only LB medium), 1% by weight, 3% by weight, 6% by weight 5% and 10% by weight were prepared, and non-pathogenic E. coli DH5α was cultured. Specifically, after adding a predetermined amount of Aso limonite to the LB medium, autoclaving at 121 ° C. for 1 hour, quantitatively inoculating non-pathogenic Escherichia coli DH5α, autoclaving at 121 ° C. for 1 hour, and then standing still at 37 ° C. for 12 hours. The result was confirmed. The results are shown in FIGS. FIG. 1 is a photograph of each soft agar medium, and FIG. 2 shows the colony formation rate in each petri dish shown in this photograph.
これらの結果、大腸菌育成試験において、阿蘇リモナイトが添加されていないものに対し、阿蘇リモナイトを添加したものはいずれもコロニー形成率が大幅に軽減されていることを確認した。具体的には、１重量％でも阿蘇リモナイトが含まれていれば阿蘇リモナイトを含んでいないものに比べ大幅にコロニー形成数を軽減できていることを確認し、特に６重量％以上、好ましくは１０重量％以上含むことで更に大腸菌コロニー形成を抑制することができることを確認した。 As a result, in the Escherichia coli breeding test, it was confirmed that the colony formation rate was significantly reduced in any of the cases where Aso limonite was not added to those in which Aso limonite was added. Specifically, if 1% by weight of Aso limonite is contained, it is confirmed that the number of colonies formed can be greatly reduced as compared with that containing no Aso limonite, particularly 6% by weight or more, preferably 10%. It was confirmed that the colony formation can be further suppressed by containing at least wt%.
（大腸菌増殖抑制効果：Ｏ１５７）
次に、寒天培地と阿蘇リモナイトの合計重量を１００重量％とした場合に、阿蘇リモナイトの重量が１０重量％となるよう作製した培地に、病原性大腸菌Ｏ１５７を播種し、３７℃で２４時間静置した後のコロニーの数、コロニーサイズ及び増殖効率の測定を行った。また、この比較例として、（Ａ）寒天培地のみの培地、（Ｂ）寒天培地とＦｅ２Ｏ３の合計重量を１００重量％とした場合にＦｅ２Ｏ３の重量が７重量％となるよう作製した培地、（Ｃ）寒天培地とＦｅＯ（ＯＨ）の合計重量を１００重量％とした場合にＦｅＯ（ＯＨ）の重量が７重量％となるよう作製した培地、（Ｄ）寒天培地とＳｉＯ２の合計重量を１００重量％とした場合にＳｉＯ２の重量が１．３７重量％となるよう作製した培地、（Ｅ）寒天培地とＡｌ２Ｏ３の合計重量を１００重量％とした場合にＡｌ２Ｏ３の重量が０．２７８重量％となるよう作製した培地、（Ｆ）寒天培地とＭｇＯの合計重量を１００重量％とした場合にＭｇＯの重量が０．０５１重量％となるよう作製した培地、をそれぞれ作製し、上記と同様の測定を行った。この結果を図３乃至７に示す。図３は各培地における写真であり、図４はそのそれぞれの一部拡大写真である。また図５はこの写真において示される各培地のコロニー数に関する測定結果を、図６はこの写真において示されるコロニーサイズの測定結果を示す。
(E. coli growth inhibitory effect: O157)
Next, when the total weight of the agar medium and Aso limonite is 100% by weight, the pathogenic Escherichia coli O157 is inoculated on the medium prepared so that the weight of Aso limonite is 10% by weight, and the plate is allowed to stand at 37 ° C. for 24 hours. The number of colonies after placement, colony size, and growth efficiency were measured. As a comparative example, when (A) a medium containing only agar medium and (B) the total weight of the agar medium and Fe 2 O 3 is 100% by weight, the weight of Fe 2 O 3 is 7% by weight. The prepared medium, (C) the medium prepared so that the weight of FeO (OH) becomes 7% by weight when the total weight of the agar medium and FeO (OH) is 100% by weight, (D) the agar medium and SiO 2 A medium prepared such that the weight of SiO 2 is 1.37% by weight when the total weight of is 100% by weight, (E) Al when the total weight of the agar medium and Al 2 O 3 is 100% by weight A medium prepared so that the weight of 2 O 3 is 0.278% by weight. (F) When the total weight of the agar medium and MgO is 100% by weight, the weight of MgO is 0.051% by weight. Medium as above, as above Measurements were carried out. The results are shown in FIGS. FIG. 3 is a photograph of each medium, and FIG. 4 is a partially enlarged photograph of each. FIG. 5 shows the measurement results regarding the number of colonies of each medium shown in this photograph, and FIG. 6 shows the measurement results of the colony size shown in this photograph.
この結果、大腸菌Ｏ１５７に対して、阿蘇リモナイトを含有する培地ではコロニー数を抑制する傾向があるだけでなく、コロニーサイズを顕著に抑制することが確認でき、この結果、大腸菌増殖を極めて効果的に抑制することができることを確認した。特に、阿蘇リモナイトに含まれる各要素では大腸菌に対する増殖抑制能は十分ではないと考えられる一方、Ｆｅ酸化物、ＳｉＯ２、Ａｌ２Ｏ３、ＣａＯを所定量混合してなる阿蘇リモナイトを用いることで、相乗的に大腸菌に対する増殖抑制能を顕著に発揮することができると考えられる。 As a result, it can be confirmed that the medium containing Aso limonite not only has a tendency to suppress the number of colonies but also remarkably suppresses the colony size with respect to E. coli O157. It was confirmed that it can be suppressed. In particular, Aso while in each element in limonite is considered not sufficient ability to suppress proliferation against E. coli, Fe oxide, the use of SiO 2, Al 2 O 3, the CaO formed by mixing predetermined amounts Aso limonite Thus, it is considered that the ability to suppress the growth of Escherichia coli can be exerted synergistically.
（大腸菌増殖抑制効果：Ｏ１３８）
ここで、上記大腸菌Ｏ１５７を大腸菌Ｏ１３８に変えた以外は同じ条件で同様の測定を行った。この結果を図７乃至９に示す。図７は、各培地の一部拡大写真である。また図８はコロニー数に関する測定結果を示す図であり、図９はコロニーサイズに関する測定結果を示す図である。
(E. coli growth inhibitory effect: O138)
Here, the same measurement was performed under the same conditions except that E. coli O157 was changed to E. coli O138. The results are shown in FIGS. FIG. 7 is a partially enlarged photograph of each medium. Moreover, FIG. 8 is a figure which shows the measurement result regarding the number of colonies, and FIG. 9 is a figure which shows the measurement result regarding colony size.
本結果によっても、阿蘇リモナイトによって、上記Ｏ１５７と同様の効果を確認することができ、リモナイトによって極めて効果的に大腸菌の増殖を極めて効果的に抑制することができるのを確認した。 Also from this result, it was confirmed that the same effect as O157 was confirmed by Aso limonite, and the growth of E. coli was extremely effectively suppressed by limonite.
（大腸菌増殖抑制効果：ＤＨ５α）
また、上記と同様の培地を準備し、上記非病原性大腸菌ＤＨ５αに対しても同様に増殖抑制効果を確認した。図１０に大腸菌増殖抑制効果の結果について示しておく。本結果によっても、コロニー数及びコロニーサイズの減少が確認でき、この結果の大腸菌増殖抑制の顕著な効果を確認することができた。
(E. coli growth inhibitory effect: DH5α)
Moreover, the same culture medium as the above was prepared and the growth inhibitory effect was confirmed similarly also with respect to the non-pathogenic E. coli DH5α. FIG. 10 shows the results of the E. coli growth inhibitory effect. Also from this result, the decrease in the number of colonies and the colony size could be confirmed, and the remarkable effect of inhibiting the growth of E. coli as a result could be confirmed.
以上、本実施例によって、リモナイトが大腸菌増殖抑制剤として機能することを確認した。なお、阿蘇リモナイトを混合した溶液のｐＨはやや酸性に傾いてはいるが、ＮａＯＨ等の塩基によって中性に調整しても同様の効果を得ることができる。 As described above, it was confirmed by this example that limonite functions as an Escherichia coli growth inhibitor. The pH of the solution mixed with Aso limonite is slightly acidic, but the same effect can be obtained even if it is neutralized with a base such as NaOH.
本発明は、大腸菌増殖抑制剤及び大腸菌増殖抑制方法として産業上の利用可能性がある。
The present invention has industrial applicability as an Escherichia coli growth inhibitor and an Escherichia coli growth inhibitory method.
Claims (5)
前記リモナイトは、前記リモナイトの全量を１００重量部とした場合に、Ｆｅ酸化物を６０重量部以上８０重量部以下、ＳｉＯ２を８重量部以上１８重量部以下、Ａｌ２Ｏ３を１重量部以上５重量部以下、ＣａＯを０．１重量部以上２重量部以下含む大腸菌増殖抑制剤（生活用水の浄水に係るものを除く）。 An E. coli growth inhibitor containing limonite,
The limonite is 60 parts by weight to 80 parts by weight of Fe oxide, 8 parts by weight to 18 parts by weight of SiO 2 , and 1 part by weight of Al 2 O 3 when the total amount of the limonite is 100 parts by weight. E. coli growth inhibitor containing 5 parts by weight or less and 0.1 part by weight or more and 2 parts by weight or less of CaO (excluding those related to the purification of domestic water) .
前記リモナイトは、前記リモナイトの全量を１００重量部とした場合に、Ｆｅ酸化物を６０重量部以上８０重量部以下、ＳｉＯ２を８重量部以上１８重量部以下、Ａｌ２Ｏ３を１重量部以上５重量部以下、ＣａＯを０．１重量部以上２重量部以下含む大腸菌増殖抑制方法（生活用水の浄水に係るものを除く）。 A method for inhibiting E. coli growth by adding limonite to an object to be treated, and mixing them.
The limonite is 60 parts by weight to 80 parts by weight of Fe oxide, 8 parts by weight to 18 parts by weight of SiO 2 , and 1 part by weight of Al 2 O 3 when the total amount of the limonite is 100 parts by weight. An E. coli growth suppression method comprising 5 parts by weight or less and 0.1 part by weight or more and 2 parts by weight or less of CaO (excluding those related to the purification of domestic water) .
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