JP6420047B2 - Fermented malt beverage - Google Patents

Description

  The present invention is a fermented malt beverage having a refreshing sweetness and a gorgeous fragrance even though the use ratio of malt to fermentation raw material is low and malt off-flavor (malt-derived off-flavor, malt odor) is low About.

  Beer is a typical liquor that has been loved around the world since ancient times. Further, as a new alcoholic beverage following beer, beer-taste beverages using various auxiliary materials other than malt such as happoshu are actively developed. Much of the response and richness peculiar to fermented malt beverages such as beer depend on malt-derived flavor components. For example, a fermented malt beverage having a low use ratio of malt can achieve a refreshing taste, but tends to lack a taste and richness compared to beer.

  On the other hand, malt contains many components with unique odors called “wheat odor” and “grain odor”, and in malt beverages made from malt, this malt off-flavor is a sensory problem. It has become. In particular, in a fermented malt beverage mainly characterized by a feeling of thirst and a drinkability, malt off-flavor tends to work more negatively. That is, when the malt use ratio is lowered, malt-derived off-flavor can be reduced, but beer-like flavor and richness are also lowered.

  Additives for imparting or enhancing beer-like flavor and richness are also known. For example, Patent Document 1 includes at least one alcohol-based component selected from the group consisting of diethoxyethane (acetal), 2-phenylethyl alcohol and 2-methyl-1-propanol, sugaralone, sotron, furaneol, Contains at least one sweetening ingredient selected from the group consisting of γ-nonalactone and γ-decalactone, and at least one aldehyde ingredient selected from the group consisting of vanillin, 3-methylbutanal and phenylacetaldehyde A flavor improver for beer-like beverages is described.

JP2013-81417A

  An object of the present invention is to provide a fermented malt beverage having a refreshing sweetness and a gorgeous fragrance even though the use ratio of malt to a fermentation raw material is low and malt off-flavor is low.

As a result of diligent research to solve the above-mentioned problems, the present inventors have found that in a fermented malt beverage in which the use ratio of malt to fermentation raw materials is suppressed to less than 25% by mass in order to suppress malt off-flavor, phenylethyl alcohol , cis-3-hexenol (cis-3-hexenol), 4- methyl-3-penten-1-ol (4-methyl-3-penten -1-ol), 3- phenylpropionic acid ethyl (ethyl 3 -Phenylpropionate), 3-mercapto-1-hexanol (3-MH), and benzaldehyde and linalool content are adjusted within a predetermined range, thereby refreshing sweetness And the present invention was completed.

That is, the fermented malt beverage according to the present invention includes the following [1] to [4].
[1] The malt ratio of the fermentation raw material is less than 25% by mass, the content of phenylethyl alcohol is 21 to 30 ppm, the content of cis-3-hexenol is 0.5 to 0.6 ppb, 4 -The content of methyl-3-penten-1-ol is 0.9 to 1.5 ppb, the content of ethyl 3-phenylpropionate is 1.2 to 1.5 ppb, and 3-mercapto- A fermented malt beverage characterized by having a 1-hexanol content of 27 to 35 ppt, a benzaldehyde content of 1 to 8 ppb, a linalool content of 4 to 8 ppb, and a beer-taste beverage.
[2] a content of phenylethyl alcohol 21~27ppm, 3- mercapto-1-a content of hexanol 27～33Ppt, a content of benzaldehyde is 1～5Ppb, linalool content of 6 The fermented malt beverage according to [1], which is 8 ppb.
[3] The fermented malt beverage according to [1] or [2], wherein hops are used as a raw material.
[4] Phenylethyl alcohol, cis-3-hexenol, 4-methyl-3-penten-1-ol, ethyl 3-phenylpropionate, 3-mercapto-1-hexanol, benzaldehyde, or linalool is contained as a raw material. The fermented malt beverage according to any one of [1] to [3].

  According to the present invention, it is possible to obtain a fermented malt beverage having a small amount of malt off-flavor and having a refreshing sweet taste and a gorgeous aroma.

  In this invention and this-application specification, a fermented malt drink means the drink which uses malt as a fermentation raw material and is manufactured through a fermentation process. The fermented malt beverage according to the present invention is a sparkling beverage having a flavor, taste and texture equivalent to or similar to beer regardless of the alcohol content, and having a high feeling of thirst and drinkability. That is, the fermented malt beverage according to the present invention may be an alcoholic beverage, or a so-called non-alcoholic beverage or low-alcoholic beverage having an alcohol content of less than 1% by volume. Specific examples of the fermented malt beverage include beer-taste beverages such as beer, sparkling liquor using malt as a raw material, low alcohol sparkling beverage, and non-alcohol beer. In addition, liqueurs obtained by mixing malt as a raw material and a beverage produced through a fermentation process with an alcohol-containing distillate may be used. The alcohol-containing distillate is a solution containing an alcohol obtained by a distillation operation, and those generally classified as spirits such as spirits can be used.

  Although the fermentation raw material for manufacturing the fermented malt drink which concerns on this invention contains malt, the malt ratio is less than 25 mass%. By using malt as a fermentation raw material, a fermented malt beverage with a clearer beer taste can be produced. Moreover, the fermented malt drink with few malt off-flavors is obtained because the malt ratio which occupies for a fermentation raw material is low. The malt used for the fermentation raw material may be barley malt or wheat malt. Moreover, although it can also be used as a syrup, an extract, etc., it is preferable to use as a malt ground material obtained by grind | pulverizing. Examples of the pulverized malt include barley, for example, Nijo barley, germinated by a conventional method, dried, and then pulverized to a predetermined particle size.

  Some malt-derived ingredients play an important role in achieving a beer taste. For this reason, beer-taste beverages with a low malt ratio have a problem that although the malt off-flavor is lower than beer-taste beverages with a high malt ratio such as beer, the flavor unique to beer tastes is reduced.

Fermented malt beverages according to the present invention, the content of phenylethyl alcohol 21～30Ppm, preferably 21～27Ppm, cis-3 content of hexenol is 0.4～0.8Ppb, preferably 0 0.5 to 0.6 ppb, and the content of 4-methyl-3-penten-1-ol is 0.8 to 1.6 ppb, preferably 0.9 to 1.5 ppb, and ethyl 3-phenylpropionate Content of 0.5 to 2.0 ppb, preferably 0.9 to 1.5 ppb, content of 3-mercapto-1-hexanol is 27 to 35 ppt, preferably 27 to 33 ppt, and content of benzaldehyde The amount is 1-8 ppb, preferably 1-5 ppb. Since the fermented malt beverage according to the present invention contains these six kinds of specific flavor components in a specific blending ratio in a well-balanced manner, the fermented malt beverage has a refreshing sweetness despite a low use ratio of malt.

Phenylethyl alcohol fermented malt beverages according to the present invention, cis-3-hexenol, the content of 4-methyl-3-penten-1-ol, 3-phenylpropionic acid ethyl and 3-mercapto-1-hexanol Can be measured, for example, by GC / MS analysis using dichloromethane liquid-liquid extraction. Specifically, first, a sample is collected in a container, ammonium sulfate is added, then dichloromethane is added to the container and an internal standard substance is added, followed by extraction with shaking. At this time, if there is gas in the container, it is preferable to perform degassing. Thereafter, centrifugation is performed to recover the solvent layer. The recovered solvent layer is dehydrated by adding anhydrous sodium sulfate, and then concentrated by nitrogen purge, and then subjected to GC / MS analysis.

  The content of benzaldehyde can be determined by, for example, on-fiber derivatization solid-phase microextraction method (Vesely) using PFBOA (O- (2,3,4,5,6-pentafluorobenzyl) hydroxylamine hydrochloride) as a derivatization reagent. Et al., Journal of Agricultural and Food Chemistry, 2003, Vol. 51, No. 24, pp. 6941-6944.). PFBOA selectively reacts with carbonyl compounds to form oximes. The formed oxime is detected by GC / MS analysis.

Phenylethyl alcohol, cis-3-hexenol, 4-methyl-3-penten-1-ol, 3-phenylpropionic acid ethyl, 3-mercapto-1-hexanol, and the content of benzaldehyde, conventional malt ratio as a method of modulating in high the concentration range than fermented malt beverages of less than 25 wt%, for example, raw materials of fermented malt beverages such as malt, originally phenylethyl alcohol, cis-3-hexenol, 4-methyl Select and use at least one kind of -3-penten-1-ol, ethyl 3-phenylpropionate, 3-mercapto-1-hexanol, or benzaldehyde higher than other varieties of the same kind A method is mentioned. Further, synthetic or phenylethyl alcohol from natural products were extracted and purified, cis-3-hexenol, 4-methyl-3-penten-1-ol, 3-phenylpropionic acid ethyl, 3-mercapto-1- Hexanol or benzaldehyde itself may be added as an additive, and a fragrance containing at least one of these six flavor components may be added as an additive.

  The fermented malt beverage according to the present invention has a linalool content of 4 to 8 ppb. By adjusting the linalool content within the above range, a gorgeous scent is enhanced and a fermented malt beverage with high palatability is obtained. The linalool content of the fermented malt beverage according to the present invention is more preferably 6 to 8 ppb. In addition, the linalool content in fermented malt drink can be quantified by GC-MS (gas chromatograph mass spectrometry).

  Examples of a method for adjusting the linalool content within the above range include a method using a material containing linalool as a raw material. For example, the linalool content of the fermented malt beverage can be adjusted to 4 to 8 ppb by using an appropriate amount of hops of varieties containing linalool as raw materials. Further, linalool itself, which is synthetic or extracted / purified from a natural product, may be added as an additive, and a fragrance containing linalool may be added as an additive.

Fermented malt beverages according to the present invention, phenylethyl alcohol, cis-3-hexenol, 4-methyl-3-penten-1-ol, 3-phenylpropionic acid ethyl, 3-mercapto-1-hexanol, benzaldehyde, And other than adjusting the content of linalool within a predetermined range, other malt can be used as a raw material, and it can be produced in the same manner as in the production method of beer, happoshu and the like produced through a fermentation process. Specifically, for example, it can be produced by the steps of preparation (sugar solution preparation), fermentation, storage, and filtration.

  First, as a preparation step (sugar solution preparation step), a sugar solution is prepared from a fermentation raw material containing malt. Specifically, a mixture containing a fermentation raw material containing malt and raw water is prepared and heated to saccharify the starch material of the fermentation raw material.

  As a fermentation raw material other than malt, only a cereal raw material may be used, only a saccharide raw material may be used, or both may be used in combination. Examples of the grain raw material include barleys other than malt such as barley and wheat, beans such as rice, corn and soybeans, and potatoes, and barley is particularly preferable. The grain raw material can be used as a grain syrup, a grain extract or the like, but is preferably used as a ground grain product obtained by a grinding process. Grain pulverization can be carried out by a conventional method. The pulverized cereal may be a corn starch, corn grits, or the like that has been subjected to a treatment usually performed before and after the pulverization treatment. Moreover, as a grain raw material other than malt used in the present invention, one kind of grain raw material may be used, or a mixture of a plurality of kinds of grain raw materials may be used. For example, a mixture of a malt pulverized product and a pulverized product of rice or corn may be used as a fermentation raw material. Moreover, it is also preferable that at least a part thereof includes a germinated state. Examples of the saccharide raw material include saccharides such as liquid sugar.

  Other auxiliary materials may be added to the mixture containing the fermentation raw material and raw water. Examples of the auxiliary material include hops, dietary fiber, fruit juice, bitters, coloring agents, herbs, and fragrances. Moreover, enzyme agents, such as saccharification enzymes, such as (alpha) -amylase, glucoamylase, and pullulanase, and protease, can be added as needed.

  The saccharification treatment is performed using an enzyme derived from a grain raw material such as malt or an enzyme added separately. The temperature and time during the saccharification treatment take into account the type of cereal raw material used, the proportion of cereal raw material in the total fermentation raw material, the type of enzyme added and the amount of the mixture, the quality of the desired fermented malt beverage, etc. , Adjusted as appropriate. For example, the saccharification treatment can be performed by a conventional method such as holding a mixture containing malt or the like at 35 to 70 ° C. for 20 to 90 minutes.

  By boiling the sugar solution obtained after the saccharification treatment, a boiled juice (boiled sugar solution) can be prepared. The sugar solution is preferably filtered before boiling, and the obtained filtrate is preferably boiled. Further, a mixture of malt extract and other sugar liquid added with warm water may be used in place of the filtrate of the sugar liquid obtained after the saccharification treatment or in addition to the filtrate and boiled. The boiling method and its conditions can be determined as appropriate.

  A fermented malt beverage having a desired flavor can be produced by appropriately adding herbs before or during the boiling treatment. In particular, hops are preferably added before or during the boiling process. By boiling in the presence of hops, it is possible to efficiently boil flavor and aroma components of hops including polyphenols. It is preferable that the amount of hops added, the mode of addition (for example, adding in several portions), the boiling conditions, etc. are appropriately adjusted in consideration of the intended quality of the fermented malt beverage as the final product.

  After the preparation process and before the fermentation process, it is preferable to remove protein and other soot produced by precipitation from the prepared broth. The removal of the soot may be performed by any solid-liquid separation process, but in general, the precipitate is removed using a tank called a whirlpool. The temperature of the broth at this time should just be 15 degreeC or more, and is generally performed at about 50-80 degreeC. The broth (filtrate) after removing the koji is cooled to an appropriate fermentation temperature with a plate cooler or the like.

  Next, as a fermentation process, yeast is inoculated into the cooled filtrate to perform fermentation. The cooled filtrate may be subjected to the fermentation process as it is, or may be subjected to the fermentation process after adjusting to a desired extract concentration. The yeast used for fermentation is not particularly limited, and can be appropriately selected from yeasts used for producing alcoholic beverages. Although it may be a top fermentation yeast or a bottom fermentation yeast, it is preferably a bottom fermentation yeast because it can be easily applied to large-scale brewing facilities.

  Moreover, the amount of alcohol produced | generated by fermentation is reduced more by suppressing alcoholic fermentation in a fermentation process. Therefore, particularly when producing a fermented malt beverage having a very low alcohol concentration, such as a non-alcohol beer having an alcohol concentration of less than 1% by volume, it is also preferable to lower the degree of fermentation in the fermentation process.

  Furthermore, as a sake storage process, the obtained fermented liquor is aged in a storage tank, stored and stabilized under a low temperature condition of about 0 ° C., and then filtered as a filtration process. The target fermented malt beverage can be obtained by removing yeast and proteins insoluble in the temperature range. The said filtration process should just be a method which can filter and remove yeast, for example, diatomaceous earth filtration, filter filtration with a filter whose average pore diameter is about 4-5 micrometers, etc. are mentioned.

  Moreover, in order to make it a desired alcohol concentration, you may dilute by performing an appropriate amount of water after filtration. The obtained fermented malt beverage is usually bottled by a filling process and shipped as a product.

  In addition, the fermented malt drink equivalent to the liqueur in liquor tax law can be manufactured, for example by mixing with spirits in the process after the fermentation process by yeast. The addition of spirits may be before or after addition for adjusting the alcohol concentration. Since the spirit to add can manufacture the fermented malt drink which has a more preferable malt feeling, a wheat spirit is preferable.

Phenylethyl alcohol, cis-3-hexenol, 4-methyl-3-penten-1-ol, 3-phenylpropionic acid ethyl, 3-mercapto-1-hexanol, benzaldehyde, or as linalool, synthetic or natural product In the case of adding the extracted / purified product from the raw material as a raw material, the timing of adding these is not particularly limited as long as phenylethyl alcohol or the like can remain in the final product. Since the risk of loss due to decomposition, modification, volatilization, etc. is small after addition, it is preferable to add phenylethyl alcohol or the like to the sugar solution after saccharification treatment, the fermentation solution after fermentation, or the filtrate after filtration. More specifically, when added to the sugar solution, it may be added immediately after the saccharification treatment, may be added to the boiled sugar solution (boiled juice), or added to the sugar solution after the precipitate is removed after boiling. Alternatively, it may be added to the cooled sugar solution before yeast inoculation.

  EXAMPLES Next, although an Example and a reference example are shown and this invention is demonstrated further in detail, this invention is not limited to a following example etc.

[Reference Example 1]
The influence of the malt ratio on the strength of malt off-flavor in fermented malt beverages of beer taste was investigated using malt pulverized product and corn starch as fermentation raw materials. Specifically, a mixture obtained by mixing a malt pulverized product and corn starch so that the malt ratio was 20, 40, or 60% by mass was used as a fermentation raw material.
First, a fermented malt beverage was produced using a 200 L scale preparation facility. Into the charging tank, 40 kg of fermentation raw material and 160 L of raw material water were added, and the mixture in the charging tank was heated according to a conventional method to produce a saccharified solution. The obtained saccharified solution was filtered, and hops were added to the obtained filtrate, followed by boiling to obtain wort (cereal broth). Next, the wort of about 80 to 99 ° C. was transferred to a settling tank to separate and remove the precipitate, and then cooled to about 7 ° C. The cold wort was inoculated with beer yeast, fermented at about 10 ° C. for 7 days, and then aged in a storage tank for 7 days. The fermented liquor after aging was filtered (average pore size: 0.65 μm) to obtain the desired fermented malt beverage.

  About the malt off-flavor of the obtained fermented malt drink, the sensory test by the 6 trained beer specialty panelists was performed. As a result, the malt off-flavor was felt in the fermented malt beverage having a malt ratio of 60% by mass, but the malt off-flavor was hardly felt in the fermented malt beverage having a malt ratio of 40% by mass, and the fermented malt beverage having a malt ratio of 20% by mass. So I didn't feel malt off flavor. That is, it was confirmed that malt off-flavor was reduced by the reduction of the malt ratio with respect to the fermentation raw material.

[Example 1]
Phenylethyl alcohol, cis-3-hexenol, 4-methyl-3-penten-1-ol, 3-phenylpropionic acid ethyl, 3-mercapto-1-hexanol, benzaldehyde, and the fermented malt beverages containing linalool The blending balance of each flavor component and the effect on flavor were investigated.
Specifically, a mixture malt ratio were mixed malt pulverized material and cornstarch so that 24 wt%, used as a fermentation material, to cold wort before brewer's yeast inoculation, phenylethyl alcohol, cis-3-hexyl Se The final concentration in the beverage is as shown in Table 1 for diol, 4-methyl-3-penten-1-ol, ethyl 3-phenylpropionate, 3-mercapto-1-hexanol, benzaldehyde, and linalool (both flavors). A fermented malt beverage was produced in the same manner as in Reference Example 1 except that it was added as described above.

The content of fermented phenylethyl alcohol malt beverage, cis-3-hexenol, 4-methyl-3-penten-1-ol, 3-phenylpropionic acid ethyl and 3-mercapto-1-hexanol ( Concentration) was measured by GC / MS analysis using dichloromethane liquid-liquid extraction. Specifically, first, a sample was collected in a container, ammonium sulfate was added, then dichloromethane was added to the container and an internal standard substance was added, followed by extraction with shaking. At this time, when there was gas in the container, degassing was performed. Thereafter, centrifugation was performed to recover the solvent layer. The recovered solvent layer was dehydrated by adding anhydrous sodium sulfate, and concentrated by nitrogen purge, and then subjected to GC / MS analysis.

  The content of benzaldehyde was determined by the method of Vesely et al. (On-fiber derivatization solid-phase microextraction method using PFBOA as a derivatization reagent, Journal of Agricultural and Food Chemistry, 2003, 51, 24, 6941- 6944.).

  Moreover, the linalool content (concentration) in the fermented malt beverage was quantified by GC-MS analysis. Specifically, the aromatic component was extracted from the test sample with a C18 solid phase column, and the obtained extract was subjected to GC / MS. The internal standard method was used for quantification. Borneol was used as an internal standard substance and added so as to be 50 ppb in the sample. The analysis conditions of the hop aroma component in GC / MS were as follows.

[GC / MS analysis conditions]
Capillary column: Trade name “DB-WAX” (length 60 m, inner diameter 0.25 mm, film thickness 0.25 μm),
Oven temperature: 40 ° C. (10 minutes) → 3 ° C./minute→240° C. (20 minutes)
Carrier gas: He, 10 psi low pressure air supply,
Transfer line temperature: 240 ° C.
MS ion source temperature: 230 ° C.
MSQ pole temperature: 150 ° C
Front inlet temperature: 200 ° C
Ions used for quantification: m / z = 110 (borneol), m / z = 93 (linalool).

  About the malt off-flavor, refreshing sweetness, and gorgeousness of the obtained fermented malt drink, the sensory test by the 6 beer panelists trained by beer was conducted. As a result, in any fermented malt beverage, malt off-flavor was not felt so much. Moreover, Table 1 shows the average of the evaluations of the six panelists regarding the refreshing sweetness, gorgeousness, and comprehensive evaluation for each fermented malt beverage.

As shown in Table 1, the refreshing sweetness of the fermented malt beverage was not good in Test Zone B where the contents of all five components other than ethyl 3-phenylpropionate were low, whereas the content of phenylethyl alcohol There is 21～30Ppm, cis-3-a content of hexenol is 0.4～0.8Ppb, the content of 4-methyl-3-penten-1-ol 0.9~1.5ppb The content of ethyl 3-phenylpropionate is 0.5 to 2.0 ppb, the content of 3-mercapto-1-hexanol is 28 to 33 ppt, and the content of benzaldehyde is 1 to 8 ppb. The test sections A and C to E were good. In particular, a 21~27ppm content of phenylethyl alcohol, cis-3-a content of hexenol is 0.5～0.6Ppb, 4-methyl-3-penten-1-content of all Is 1.0 to 1.3 ppb, the content of ethyl 3-phenylpropionate is 0.9 to 1.5 ppb, the content of 3-mercapto-1-hexanol is 28 to 33 ppt, In the test sections A and C whose contents were 2 to 5 ppb, they had a very good and refreshing sweetness.

  Moreover, although the gorgeous fragrance of the fermented malt beverage was hardly observed in the test section B having a linalool content of 0.5 ppb, it was good in the test section E having 4 ppb, and very good in the test sections A and C having 6-8 ppb. It was very good. However, the test group D having a linalool content of 15 ppb had a strong scent peculiar to linalool and was weaker than the test group E of 4 ppb, and was normal.

  According to the present invention, it is possible to obtain a fermented malt beverage having a refreshing sweetness and a gorgeous fragrance despite a small amount of malt off-flavor. Is possible.

Claims (4)

Fermented wort ratio of the raw material is less than 25 wt%, a content of phenyl ethyl alcohol 21～30Ppm, content of cis-3-hexenol is 0.5 ~ 0.6 ppb, 4-methyl - The content of 3-penten-1-ol is 0.9 to 1.5 ppb, the content of ethyl 3-phenylpropionate is 1.2 to 1.5 ppb, and 3-mercapto-1-hexanol. The fermented malt beverage is characterized in that it is a beer-taste beverage having a benzaldehyde content of 27 to 35 ppt, a benzaldehyde content of 1 to 8 ppb, a linalool content of 4 to 8 ppb. The phenylethyl alcohol content is 21-27 ppm, the 3-mercapto-1-hexanol content is 27-33 ppt, the benzaldehyde content is 1-5 ppb, and the linalool content is 6-8 ppb. The fermented malt beverage according to claim 1.   The fermented malt beverage according to claim 1 or 2, wherein hops are used as a raw material.   It contains phenylethyl alcohol, cis-3-hexenol, 4-methyl-3-penten-1-ol, ethyl 3-phenylpropionate, 3-mercapto-1-hexanol, benzaldehyde, or linalool as a raw material. The fermented malt beverage according to any one of Items 1 to 3.