JP6369899B2 - Intraoral massage effect evaluation method - Google Patents

Description

  The present invention relates to an intraoral massage effect evaluation method for evaluating the effect of intraoral massage using an intraoral massage tool.

  Conventionally, in order to maintain health in the oral cavity, oral massage that improves the blood circulation by massaging the mucous membrane in the oral cavity such as gums and cheeks is often performed. Among them, the inventor of the present application has proposed a gum massage tool (oral massage tool) that can promote blood circulation of the gums in Patent Document 1.

International application WO2012 / 063835

  However, there is no established method for evaluating the effect of intraoral massage.

  This invention is made | formed in view of the reason which concerns, The objective is to provide the intraoral massage effect evaluation method which can evaluate an effect appropriately in the intraoral massage using an intraoral massage tool.

  In order to achieve the above object, the method for evaluating the effect of intraoral massage according to claim 1 forms an ulcer by applying or injecting an acidic solution to the mucous membrane in the oral cavity of a laboratory animal, Intraoral massage is performed using an intraoral massage tool having an intraoral contact portion having a molded body, and then the tissue of the mucous membrane that has formed the ulcer is removed and stained to obtain cell cycle-related protein or heat. By observing a range showing a positive reaction of shock protein, the regeneration reaction of the mucosal tissue or the protective reaction against heat stress is evaluated as an effect of oral massage.

  The oral massage effect evaluation method according to claim 2 is the oral massage effect evaluation method according to claim 1, wherein the cell cycle-related protein is Ki67 and the heat shock protein is HSP27 or HSP70. And

  ADVANTAGE OF THE INVENTION According to this invention, the intraoral massage effect evaluation method using the intraoral massage tool which can evaluate an effect appropriately can be provided.

The cross section of the intraoral massage tool used for the intraoral massage effect evaluation method which concerns on embodiment of this invention is shown, Comprising: (a) is typical sectional drawing, (b) is expansion of the vicinity of an intraoral contact part. It is sectional drawing. It is a reduced perspective view of the intraoral massage device of the above, wherein (a) is a perspective view and (b) is a perspective view observed from the bottom side. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and is about Ki67 of specimen A. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and it is about the Ki67 of the sample B. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and it is about Ki67 of the sample C. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and it is about HSP27 of specimen A. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and it is about HSP27 of specimen B. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and it is about HSP27 of specimen C. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and is about HSP70 of specimen A. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and is about HSP70 of specimen B. The photograph of the result of the experiment of an intraoral massage effect evaluation method same as the above is shown, and it is about HSP70 of the sample C.

  Hereinafter, modes for carrying out the present invention will be described. The intraoral massage effect evaluation method according to the embodiment of the present invention is a method for evaluating the effect of intraoral massage using the intraoral massage tool 1.

  The intraoral massage device 1 includes an intraoral contact portion 2. The intraoral contact part 2 is a part that is pressed against the mucous membrane in the oral cavity such as a gum or cheek as an intraoral massage. The intraoral contact portion 2 has a carbon molded body 21 as a main member.

The carbon molded body 21 has a carbon crystal structure that is dense and arranged by firing, and is formed integrally as a whole. The crystal structure can be made substantially isotropic with no bias in the overall direction. The carbon molded body 21 can be manufactured as follows. That is, about 5% by weight of a binder is added to graphite or carbide (such as bamboo charcoal) carbon powder to be solidified, and then isotropically pressed with water pressure or the like to form a predetermined lump (for example, a rectangular shape). And it heats in the state which oxygen deficient, and baks over about 2500 to 3500 degreeC over a long time. In this state, the raw material carbon powders are bonded to each other, and the carbon is crystallized (graphitized and shaped carbonized). The binder is volatilized at about 1000 to 1200 ° C. during the temperature increase. Thereafter, heating is stopped, the temperature is lowered, and processing is performed into a desired shape. Since the binder is volatilized in the carbon molded body 21 thus completed, almost 100% (99% or more) is a carbon material. The bulk density (bulk specific gravity) is 1.77 Mg / m ³ or more.

  Since the entire carbon molded body 21 is bonded by a crystal structure, the carbon molded body 21 has high physical strength and high thermal conductivity (about 100 to 140 W / (m · K)). Also, the amount of far-infrared radiation that radiates is large and the temperature is higher.

  The carbon molded body 21 is provided with a coating 22 (for example, a thickness of about 2 to 4 μm) such as diamond-like carbon (DLC) on the surface thereof. Moreover, although the shape of the carbon molded body 21 is not limited, a substantially cylindrical shape as shown in FIGS. 1A and 1B is possible.

  The whole intraoral massage device 1 can be configured as follows. That is, the intraoral massage tool 1 includes the intraoral contact part 2, the grip part 3, the heat generating part 4, and the support part 5 described above. FIG. 1A schematically shows an electric machine member and its electric wiring (broken line portion) with respect to the internal configuration of the grip portion 3.

  The intraoral contact portion 2 receives the heat of the heat generating portion 4 from the carbon molded body 21 described above, which is the main member, and transmits the heat to the surface, that is, the portion that contacts the mucous membrane in the oral cavity. Moreover, the intraoral contact part 2 is arrange | positioned in the state which can be rotated around the front-end | tip part of the rod-shaped heat-emitting part 4 so that it may mention later. In the carbon molded body 21, an insertion hole 21a into which the heat generating portion 4 is inserted is formed around the central axis. Moreover, the intraoral contact part 2 has the connection member 23 for engaging with the support part 5 rotatably as an attachment member.

  The grip portion 3 is a long, that is, substantially box-shaped box, and the selection switch 31 is exposed so that it can be operated from the outside. In addition, the grip portion 3 accommodates batteries 32 and 32 ′, thermostats 33, a motor 34, and the like that are electromechanical members. The batteries 32, 32 ′ are energized to a heating element 42 to be described later to heat the heating part 4 to heat the heating part 4 by operating the selection switch 31, and to the motor 34 to vibrate the heating part 4. To make a rotational movement. The rotational motion of the motor 34 generates vibration, and the vibration is transmitted to the heat generating unit 4 through the vibration transmitting means 34A. The selection switch 31, the electromechanical member, and the electrical wiring thereof can be changed as appropriate according to the specifications. The thermostat 33 is for controlling the surface of the intraoral contact portion 2, that is, the portion in contact with the mucous membrane in the oral cavity, to have an appropriate temperature, and the temperature detection portion contacts the heat conductor 41 described later. The heating element 42 is energized in accordance with the temperature detected by the temperature detecting portion.

  The heat generating unit 4 includes a heat conductor 41 made of metal or the like having high heat conductivity, and a heat generating body 42 that generates heat by energizing the heat conductor 42 and heats the heat conductor 41. The heat generating part 4 is fixed to the grip part 3 and has a rod shape extending from one end thereof. As the heating element 42, for example, a nichrome wire coated with an insulating material can be used by reciprocating from the vicinity of the proximal end of the thermal conductor 41 to the vicinity of the distal end.

  The support part 5 supports the intraoral contact part 2. Moreover, the support part 5 is attached to the holding part 3, and is provided with a through hole into which the heat generating part 4 is inserted along the central axis.

  The usage method of this intraoral massage tool 1 is as follows. That is, the user operates the selection switch 31 while holding the grasping part 3 by hand, heats and / or vibrates the heat generating part 4, puts the intraoral contact part 2 into the oral cavity, and puts it in the mucous membrane such as gums or cheeks. Massage by pressing and exercising with appropriate force.

  When the heat generating part 4 is heated by the operation of the selection switch 31, the heat of the heat generating part 4 is conducted to the carbon molded body 21 of the intraoral contact part 2, and the temperature becomes high. The carbon molded body 21 directly transmits heat to the mucous membrane in the oral cavity by heat conduction and radiates far infrared rays whose amount increases as the temperature increases. This far infrared ray heats the deep part of the mucous membrane in the oral cavity. Since the temperature of the mucous membrane in the oral cavity is efficiently increased by the conduction heat and the radiant heat of far infrared rays, the circulation of the mucous membrane in the oral cavity can be further promoted by the synergistic effect with the pressure by the intraoral contact portion 2. is there.

  Further, when the heat generating portion 4 vibrates by operating the selection switch 31, the vibration is transmitted to the mucous membrane in the oral cavity via the intraoral contact portion 2. This vibration can further promote blood circulation in the mucous membrane in the oral cavity.

  Next, the intraoral massage effect evaluation method for evaluating the effect of intraoral massage using the intraoral massage tool 1 described above will be described in detail.

  In this method of evaluating the effect of intraoral massage, first, an acidic solution such as acetic acid is applied to some mucous membranes (for example, gums or cheeks) in the oral cavity of a laboratory animal such as a rat or injected by injection. To produce an ulcer. And an intraoral massage is performed using the intraoral massage tool 1 several times over the day. Thereafter, the submucosal tissue of the ulcer is excised and stained, such as immunostaining and HE staining, and shows a positive reaction of cell cycle-related proteins such as Ki67 or heat shock proteins such as HSP27 or HSP70 Observe. Thereby, the regeneration reaction of the submucosa tissue or the defense response against heat stress is evaluated as the effect of the oral massage. Ki67 is observed in activated cells. HSP27 is said to show a protective response against heat stress. HSP70 is said to be involved in the regeneration of the epidermis.

  Such an intraoral massage effect evaluation method can appropriately evaluate the effect of intraoral massage using the intraoral massage device 1.

  The experiment conducted by the inventor will be described below. In this experiment, three 7-week-old male SD rats were used as specimens A, B, and C. In Samples B and C, 99.7% acetic acid was applied to the cheeks in the oral cavity, and ulcers were produced there. Specimen A does not produce an ulcer. The specimen C vibrates the oral contact part 2 of the oral massage device 1 at a temperature of 37 ° C. for 3 minutes each at the time of preparation of the ulcer part and one day, two days, and seven days after the preparation of the ulcer part. Oral massage was applied to the ulcer. Samples A and B do not perform intraoral massage. Thereafter, the submucosa tissues of the ulcers were removed from specimens A, B, and C, and the corresponding immunostaining was performed for Ki67, HSP27, and HSP70, and changes such as increases were observed. 3 to 11 are observation photographs. Each photograph is at 100 magnification and Scale bar indicates 200 μm.

  3, 4 and 5 are photographs showing the positive reaction of Ki67 in specimens A, B and C, respectively. In FIG. 3, cells in the basal cell layer showing a positive reaction of Ki67 in the specimen A are surrounded by a broken line and indicated by an arrow Aa.

  In FIG. 4, cells in the basal cell layer showing a positive reaction of Ki67 in the sample B are surrounded by a broken line and indicated by an arrow Ba. In specimen B, epithelial tissue defects due to acetic acid, that is, ulceration (indicated by arrow Bx surrounded by a solid line in the figure) are observed, and cells in the basal cell layer are poorly positive for Ki67 and observed in a localized manner. Is done. In FIG. 4, an arrow By indicates that cells having high proliferative activity among inflammatory cells under the mucous membrane of the ulcer portion show a positive reaction.

  In FIG. 5, cells in the basal cell layer showing a positive reaction of Ki67 in the sample C are surrounded by a broken line and indicated by an arrow Ca. In the sample C, cells in the basal cell layer showing a positive reaction of Ki67 are observed in a wide range, and a part (right side portion) is also observed in a wide range in the vertical direction. Thus, the sample C is observed to have a significantly wider range showing a positive reaction of Ki67 than the sample B.

  6, 7 and 8 are photographs showing the positive reaction of HSP27 in specimens A, B and C, respectively. In FIG. 6, cells in the spinous basal cell layer showing a positive reaction of HSP27 in the specimen A are surrounded by a broken line and indicated by an arrow Ab.

  In FIG. 7, cells in the spinous basal cell layer showing a positive reaction of HSP27 in the specimen B are surrounded by a broken line and indicated by an arrow Bb. In specimen B, epithelial tissue defects due to acetic acid, that is, ulceration (indicated by arrow Bx surrounded by a solid line in the figure) are observed, and cells in the spinous basal cell layer showing a positive reaction of HSP27 are localized and observed. Is done.

  In FIG. 8, cells in the spinous basal cell layer showing a positive reaction of HSP27 in the specimen C are surrounded by a broken line and indicated by an arrow Cb. In specimen C, cells in the spinous basal cell layer showing a positive reaction of HSP27 are observed in a wide range. Thus, the sample C is observed to have a significantly wider range showing a positive reaction of HSP27 than the sample B.

  9, 10, and 11 are photographs showing the positive reaction of HSP70 in specimens A, B, and C, respectively. In FIG. 9, cells in the spinous basal cell layer showing a positive reaction of HSP70 in the specimen A are surrounded by a broken line and indicated by an arrow Ac.

  In FIG. 10, cells in the spinous basal cell layer showing a positive reaction of HSP70 in the specimen B are surrounded by a broken line and indicated by an arrow Bc. In specimen B, epithelial tissue defects due to acetic acid, that is, ulceration (indicated by arrows Bx surrounded by a solid line in the figure) are observed, and cells in the spinous basal cell layer showing a positive reaction of HSP70 are localized and observed. Is done.

  In FIG. 11, cells in the spinous basal cell layer showing a positive reaction of HSP70 in the specimen C are surrounded by a broken line and indicated by an arrow Cc. In specimen C, cells in the spinous basal cell layer showing a positive reaction of HSP70 are observed over a wide range. Thus, the sample C is observed to have a significantly wider range showing a positive reaction of HSP70 than the sample B.

  As mentioned above, although the intraoral massage effect evaluation method based on embodiment of this invention was demonstrated, this invention is not restricted to what was described in the above-mentioned embodiment, In the range of the matter described in the claim Various design changes are possible.

DESCRIPTION OF SYMBOLS 1 Intraoral massage tool 2 Intraoral contact part 21 Carbon molded object 21a Insertion hole 22 Coating 3 Gripping part 31 Selection switch 32, 33, 34 Electromechanical member 4 Heat generating part 5 Support part

Claims (2)

  Applying or injecting an acidic solution to the mucous membrane in the oral cavity of a laboratory animal to form an ulcer, and multiple times oral massage using an oral massage tool with an oral contact portion having a carbon molded body And then removing the mucosal tissue in which the ulcer was formed, staining, and observing a range showing a positive reaction of cell cycle-related protein or heat shock protein, thereby regenerating the mucosal tissue or A method for evaluating the effect of intraoral massage, comprising evaluating a defense response against heat stress as an effect of intraoral massage. In the intraoral massage effect evaluation method according to claim 1,
The cell cycle-related protein is Ki67, and the heat shock protein is HSP27 or HSP70.