JP6364246B2 - Stirring apparatus, stirring method, and automatic analyzer equipped with the stirring apparatus - Google Patents

Description

The present invention efficiently stirs a reaction solution, disperses and isolates agglomerated particles, and particularly relates to a stirrer and a container for a low-volume reaction solution, and further relates to a stirring method.

  As background art in this technical field, there is US Pat. No. 10,119,929 (Patent Document 1). This publication describes that a material containing a material can be stirred or separated with high efficiency by a rotating operation combining rotation and revolution. (Hereinafter, referred to as rotation and revolution stirring.) In this device, rotation operation combining rotation and revolution is realized by using planetary gears. Furthermore, this patent describes that stirring efficiency can be improved by installing fins on the inner wall of the container.

  Moreover, there exists Unexamined-Japanese-Patent No. 2009-273959 (patent document 2). In this publication, when the stirring deaerator is stopped, the material is contained as a stirring defoaming method capable of preventing the material from entraining new air and generating new bubbles from the material. In a stirring and defoaming device configured to be able to rotate and revolve in a state where the inside of the container is decompressed, the rotation angular velocity that decreases the rotation angular velocity of the container while decreasing the rotation / revolution angular velocity ratio of the container It describes that it includes a lowering step and an air release step for opening the inside of the container to atmospheric pressure, which is started after the rotation angular velocity lowering step.

  Moreover, there exists Unexamined-Japanese-Patent No. 2011-235201 (patent document 3). In this publication, a stirring tank supported by a single drive motor at a predetermined inclination angle α with respect to a vertical rotation main shaft is revolved around the rotation main shaft at a predetermined speed and arranged in parallel with the rotation main shaft. By rotating at a low speed around the rotating support shaft, the inclination direction of the stirring tank is changed with respect to the direction of the centrifugal force applied to the liquid to be treated in the stirring tank. As a result, the centrifugal force generated by the revolution of the agitation tank does not stop in the horizontal direction with respect to the liquid to be treated, but also moves in the vertical direction due to the cooperative action with the change in the inclination direction of the inclined inner surface of the agitation tank. It is described that the liquid to be treated flows (three-dimensional motion) in the vertical and horizontal directions in the stirring tank.

Moreover, there exists Unexamined-Japanese-Patent No. 2013-173092 (patent document 4). In this publication, the stirring container includes a container main body that contains the phenyl-based silicone resin and the phosphor powder, and a container main body holder that is provided around the container main body and has a side wall to which the container main body is fixed. The side wall is made of a material that can be deformed in the thickness direction of the side wall. Accordingly, when the container body holder rotates and revolves, the side wall of the container body holder is deformed, and the position of the container body in the container body holder moves. As a result, since the central axis of the container body changes with respect to the rotation axis, it is described that the region where the convection force is weakened on the bottom wall of the container body can be moved.

U.S. Pat. JP 2009-273959 A JP 2011-235201 A JP2013-173092A

  In biochemical / immunological test apparatuses, for the purpose of reducing test costs and reducing patient burden, it is one of the important issues to reduce the amount of reagents and the amount of blood collected as a sample. As a result of reducing the reagent amount and the sample amount, the amount of the reaction liquid in which the sample and the reagent are mixed is reduced.

  The stirred reaction liquid is introduced into the sensor unit or the like using a thin tube called a nozzle. In order to ensure that the reaction liquid is sucked without any remaining liquid by the nozzle, it is necessary to secure a certain level of the liquid level of the reaction liquid. In other words, in order to aspirate the low-volume reaction liquid reliably, it is inevitably necessary to reduce the diameter of the reaction vessel containing the reaction liquid to ensure the liquid level.

  On the other hand, the stirring device used in biochemical / immunological test devices, etc., mixes the sample and the reagent (stirring) by applying a rotating operation that combines rotation and revolution to the reaction vessel containing the reaction solution. Since the separation / isolation of particles and particles is promoted, when rotating agitation processing is performed using a reaction vessel with a reduced diameter, the behavior of the liquid surface accompanying rotation is limited due to the surface tension of the reaction solution. This may cause a problem that stirring, separation and isolation become extremely difficult.

The present invention is for solving the problems described above, and provides a stirring device and a container for efficiently stirring a low-volume reaction solution, and further provides a stirring method using them. Is.

  The configuration of the present invention for solving the above-described problems is as follows.

That is, by rotating and revolving the container, the container stirring device that stirs the liquid stored in the container, the suction nozzle for sucking the liquid stored in the container, and the suction nozzle In an automatic analyzer comprising an analyzer for analyzing the sucked liquid and a controller for controlling the operation of the container agitator, the suction nozzle and the analyzer, the container agitator has a diameter of 3.0 mm or less. A container holding unit capable of holding a container capable of storing a liquid of 100 uL or less, a revolution rotating unit that rotatably holds the container holding unit, and a rotation driving unit that rotationally drives the entire revolution rotating unit. And a rotation suppressing member that suppresses the rotation of the container holding portion with respect to the rotational drive of the revolution rotation, the revolution angular velocity is ω [rad / s], and the diameter of the container is D [m]. The distance between the bottom surface of the rotation center and the vessel L [m], the installation angle of the container relative revolution axis θ [rad], V [m 3] of the amount of liquid, the density of the liquid ρ [kg / m ^3] , Tan ⁻¹ (8V / πD ³ ) / tan ⁻¹ ((ω ² Lcos θ + g) sin θ / −, where σ [N / m] is the surface tension of the liquid and g [m / s ² ] is the gravitational acceleration. The value of (ω ² Lsin ² θ + g cos θ) is larger than 0.09 and smaller than 0.135, and (σ / (ρ (ω ⁴ L ² sin ² θ + ⁴ gω ² Lsin ² θ cos θ + g ² ) ^ (1/2 )) An automatic analyzer characterized in that the value of ^ (1/2) / D is smaller than 1.

As described above, when the present invention is used, it is possible to efficiently stir even in the case of using a thin reaction vessel that accommodates a low-volume reaction solution and considers suction by a nozzle, thereby reducing the inspection cost. The patient burden can be reduced.

It is a whole block diagram of the sample analyzer in this invention. It is a figure which shows the structural example of the reaction container stirring apparatus. It is the figure which showed the rotation operation | movement of the reaction container accompanying stirring. It is the figure which showed the definition of the design parameter variable of a stirring apparatus. It is a figure for demonstrating an ideal liquid level inclination angle. It is the figure which put together the result from which a liquid level behavior changes with the difference of two evaluation parameters. It is a figure which shows the reaction container stirring apparatus in Example 3 of this invention.

  Embodiments of the present invention will be described below with reference to the drawings.

  As an example of a sample analyzer which is one of the embodiments, an immune analyzer will be described. Note that the present invention is not limited to immunoassay, and can be applied to any sample analyzer that stirs by a rotational motion involving rotation and revolution, and can also be applied to an analyzer for DNA, biochemistry, genes, and the like. is there. An example of the configuration of the automatic immune analyzer described in this embodiment is shown in FIG.

  The control unit 119 of the analysis apparatus 100 creates an analysis plan in response to a measurement request input from an operator via a keyboard, a touch panel, or the like, and transmits the created analysis plan to the analysis apparatus 100, thereby analyzing the analysis plan. Based on the above, the operation of each mechanism described below is controlled. The control unit 119 may be provided with a storage unit that stores analysis request information, analysis parameters, analysis results, and the like.

  A sample container 102 for holding a sample is installed on the rack 101, and is moved to a sample dispensing position near the sample dispensing nozzle 103 by a rack transport line 117. A plurality of reaction containers 105 can be installed on the incubator disk 104, and the reaction containers 105 installed in the circumferential direction are arranged in a reaction container installation position, a reagent discharge position, a sample discharge position, a detection position, a reaction container discard position, For example, a rotational drive mechanism (not shown) for moving to a predetermined position is provided, and rotational movement is possible. The sample dispensing tip and reaction container transport mechanism 106 can move in three directions of the X axis, the Y axis, and the Z axis, and the sample dispensing tip and reaction container holding member 107, the reaction container stirring device 2, and the sample dispensing chip. In addition, the range of the reaction container disposal hole 109, the sample dispensing tip mounting position 110, and a predetermined portion of the incubator disk 104 is moved to carry the sample dispensing tip and the reaction container.

  The sample dispensing tip and reaction vessel holding member 107 is provided with a plurality of unused reaction vessels and sample dispensing tips. The sample dispensing tip and reaction vessel transport mechanism 106 moves above the sample dispensing tip and reaction vessel holding member 107, descends, holds and raises an unused reaction vessel, and installs the reaction vessel on the incubator disk 104. Move above position and lower to install reaction vessel.

  The reagent disk 111 is provided with a plurality of reagent containers 118 holding reagents and diluents. A reagent disk cover 112 is provided above the reagent disk 111, and the inside of the reagent disk 111 is maintained at a predetermined temperature. A reagent disk cover opening 113 is provided in a part of the reagent disk cover 112. The reagent dispensing nozzle 114 can be rotated and moved up and down. The reagent dispensing nozzle 114 rotates and moves downward above the opening 113 of the reagent disk cover 112, and the tip of the reagent dispensing nozzle 114 is moved to the reagent or dilution in a predetermined reagent container. A predetermined amount of reagent or diluent is aspirated in contact with the liquid. Next, the reagent dispensing nozzle 114 is raised and moved above the reagent discharge position of the incubator disk 104 to discharge the reagent or diluent to the reaction vessel 105.

  Next, the sample dispensing tip and reaction vessel transport mechanism 106 moves above the sample dispensing tip and reaction vessel holding member 107, descends, holds and holds an unused sample dispensing tip, and then dispenses the sample. It moves above the tip mounting position 110 and descends to install the sample dispensing tip. The sample dispensing nozzle 103 can rotate and move up and down, moves to the upper side of the sample dispensing tip mounting position 110 and descends, and attaches the sample dispensing tip to the tip of the sample dispensing nozzle 103. The sample dispensing nozzle 103 equipped with the sample dispensing tip moves down above the sample container 102 placed on the transport rack 101 and sucks a predetermined amount of the sample held in the sample container 102. The sample dispensing nozzle 103 that sucks the sample moves to the sample discharge position of the incubator disk 104 and descends, and discharges the sample to the reaction container 105 on which the reagent is dispensed on the incubator disk 104. After sample discharge, the sample dispensing nozzle 103 moves above the sample dispensing tip and the reaction container discarding hole 109 and discards the used sample dispensing tip into the disposal hole.

  The reaction container 105 from which the sample and the reagent are discharged moves to the reaction container transport position by the rotation of the incubator disk 104 and is transported to the reaction container agitator 2 by the sample dispensing tip and the reaction container transport mechanism 106. The reaction vessel stirring device 2 mixes the sample and the reagent in the reaction vessel by applying a rotational motion by rotation and revolution to the reaction vessel. After the stirring, the reaction container is returned to the reaction container transport position of the incubator disk 104 by the sample dispensing tip and the reaction container transport mechanism 106. The reaction solution suction nozzle 115 can be rotated and moved up and down, and the sample and the reagent are dispensed and mixed, and then moved above the reaction vessel 105 after a predetermined time on the incubator disk 104, and then moved down. Aspirate the reaction solution inside. The reaction liquid sucked by the reaction liquid suction nozzle 115 is sent to the detection unit 116, and the measurement object is detected. The control unit 119 derives and displays the measurement result based on the detection value of the measurement object. The reaction container 105 in which the reaction liquid has been sucked moves to the reaction container discarding position by the rotation of the incubator disk 104, and the sample dispensing chip and the reaction container disposal hole are removed from the incubator disk 104 by the sample dispensing chip and reaction container transport mechanism 106. It moves above 109 and is discarded from the disposal hole.

  In FIG. 2, the figure which showed the structure of the reaction container stirring apparatus 2 in this invention is shown.

  The reaction vessel stirring device 2 includes a container holding portion 4 having a recess capable of holding the reaction vessel 105 containing the reaction liquid 1, a rotational drive generating portion 3 such as a motor, and a revolution rotation for holding the entire vessel holding portion. It is comprised by the part 5, the bearing 6, the rotation control member 7 connected with the container holding part 4, and the suppression member (not shown in the figure) which controls rotation of the container holding part 4. The rotation drive generator 3 and the revolution rotation unit 5 are coupled by a shaft 8. As the rotation drive generation unit 3 rotates about the axis A, the revolution rotation unit 5 is the same in the same direction as the rotation drive generation unit 3. Cause rotation. In addition, the rotational drive generation part 3 and the revolution rotation part 5 may be couple | bonded by pulleys other than an axis | shaft. The revolution rotating part 5 and the container holding part 4 are fixed via a bearing 6, and the container holding part 4 can freely rotate around the axis B of the container holding part 4 serving as a rotation axis. It becomes. A recess (hole shape) for inserting the reaction container 105 is formed in the container holding part 4. One end of the rotation control member 7 is screwed and fixed to the container holding portion 4, and the operation of the other end is suppressed by the suppression member located at a position away from the reaction vessel stirring device 2, thereby the container holding portion. 4 is suppressed. The suppression member is, for example, a part of the housing of the analyzer 100 and the like, and is a member that is not rotationally driven by the rotational operation by the rotational drive generator 3.

  In FIG. 3, the figure which showed typically the rotation operation | movement of the reaction container 105 when the reaction container stirring apparatus 2 in this invention is used is shown. In this figure, the coordinate system (xy coordinates) fixed to the apparatus and the specific position of the container are explicitly shown as a rectangle for the purpose of the following description. As an example, the rotation angle of the rotational drive generator 3, that is, the revolution angle is 0 °, 90 °, 180 °, 270 °.

  In the reaction vessel agitator 2 in the present embodiment, since the rotation operation is suppressed by the rotation control member 7, the reaction vessel (square) is always in the same direction (in the figure, the x-axis direction as shown in FIG. 3A). ) On the other hand, in a state where the rotation operation is not suppressed by the rotation control member 7, the reaction vessel (square) is always directed outward in the radial direction as shown in FIG. 3 (b), and the stirring device using the rotation / revolution stirring is used. The function is lost. In this stirring apparatus, as shown in FIG. 3A, the reaction vessel 105 is rotated while maintaining a constant angle with respect to the revolution axis of the rotation drive generator 3 and the rotation axis of the vessel holder 4 while facing substantially the same direction. Will be. In addition, in this reaction container stirring apparatus 2, the rotation speed of revolution and rotation is the same, and a rotation direction becomes a reverse direction.

  Although the rotation control member 7 is composed of a rod-shaped member in the present embodiment, it does not necessarily have a rod shape. For example, the container holding portion and the suppression member are connected by a deformable ring-shaped rubber member. By doing so, the rotation of the container holding part may be controlled.

  In order to sufficiently stir the reaction liquid 1 (which may contain particles to be isolated), the liquid level of the reaction liquid 1 is such that the bottom surface of the reaction container 105 is exposed when stirring with the reaction container stirring device 2. It is necessary to lean. This is because the liquid level is inclined to the extent that the bottom surface of the reaction vessel 105 is exposed, so that the amount of movement of the reaction solution 1 in the reaction vessel 105 increases and is sufficiently stirred. On the other hand, when the reaction solution 1 does not expose the bottom surface of the reaction vessel 105, the amount of movement of the reaction solution 1 near the bottom surface of the reaction vessel 105 is suppressed, and as a result, the reaction solution 1 may not be sufficiently stirred. Therefore, it was examined how the reaction solution 1 behaves so as to expose the bottom surface of the reaction vessel 105.

In the following, the revolution angular velocity is ω [rad / s], the diameter of the reaction vessel 105 is D [m], the distance L [m] between the rotation center and the bottom surface of the reaction vessel 105, and the installation of the reaction vessel with respect to the revolution rotation axis. The angle is θ [rad], the reaction liquid volume is V [m ³ ], the reaction liquid density is ρ [kg / m ³ ], the reaction liquid surface tension is σ [N / m], and the gravitational acceleration is g [ m / s ² ].

  FIG. 4 shows a diagram showing the definition of the parameter variables of the stirring device.

  The liquid surface shape of the reaction liquid is formed so that the liquid surface shape is perpendicular to the force applied to the liquid. The force (acceleration) per unit mass applied to the liquid is a resultant force of inertial force a (vector component) and gravitational acceleration g (vector component) per unit mass. As a result, the liquid surface angle φ of the reaction liquid with respect to the bottom surface of the reaction vessel is expressed by the following equation.

[Formula 1]
φ = tan ⁻¹ ((a + g) component in the container radial direction (x, y method) component / (a + g) component in the container axis direction (z direction))
The capillary length κ ^-1 is considered to be expressed by the following equation. When the surface tension and density of the reaction solution are σ and ρ, respectively, the capillary length κ ^-1 is expressed by the following equation.

[Formula 2]
κ ⁻¹ = (σ / (ρ | a + g |)) ^ (1/2)
In calculating φ and κ ⁻¹ , it is necessary to calculate the inertial force a (vector component) per unit mass. This force can be obtained by converting the equation of motion of the absolute coordinate system into a coordinate system fixed to the reaction vessel, but becomes a very complicated equation. The term that depends on speed is small compared to other terms, so it can be ignored by simplifying it, such as ignoring it. That is, a is as follows.

[Formula 3]
container radial direction component of a = ω ² Lsin θ cos θ
Container vertical component of a = −ω ² Lsin ² θ
At this time, the gravitational acceleration is similarly as follows.

[Formula 4]
radial component of g = gsinθ
Vertical component of g = −g cos θ
If Equation 3 and Equation 4 are used, the liquid surface angle φ of the reaction liquid with respect to the bottom surface of the reaction vessel shown in Equation 1 and the capillary length κ ⁻¹ shown in Equation 2 are as follows.

[Formula 5]
φ = tan ⁻¹ ((ω ² L cos θ + g) sin θ / (− (ω ² L sin ² θ + g cos θ)))

[Formula 6]
κ ⁻¹ = (σ / (ρ (ω ⁴ L ² sin ² θ + ⁴ gω ² Lsin ² θcos θ + g ² ) ^ (1/2)) ^ (1/2)
As shown in FIG. 5 (a), a container having a diameter D is considered, and an ideal state when the liquid level is inclined by stirring the reaction liquid having a liquid volume V is considered as shown in FIG. 5 (b). Assuming that the liquid surface is inclined to the extent that the bottom surface of the container is exposed by rotation, and the angle between the bottom surface of the container and the liquid surface is ψ, the angle ψ from the container diameter D and the volume V is as follows.

[Formula 7]
ψ = tan ⁻¹ (8V / πD ³ )
In order to efficiently stir the low-volume reaction solution, the reaction solution itself needs to be moved greatly, and for this purpose, the reaction solution surface needs to be inclined to the extent that the bottom surface of the container is exposed. From this, it is considered that the low reaction liquid volume reaction liquid volume stirring is an important index that is constituted by the angle ratio of φ and ψ, and this is defined as the tilt angle parameter α.

[Formula 8]
α≡ψ / φ
Furthermore, if the capillary length κ ⁻¹ is larger than the vessel diameter D to some extent, it becomes difficult for the liquid level of the reaction vessel to expose the vessel bottom. Therefore, it is considered that a value constituted by the ratio of the capillary length κ ⁻¹ and the diameter D becomes an important index, and this is defined as the capillary parameter χ.

[Formula 9]
χ≡κ ^-1 / D
Each design parameter was changed to various values, and the liquid surface shape of the reaction liquid with respect to the values of the inclination angle parameter α and the capillary parameter χ, which are evaluation parameters, was evaluated by fluid analysis. In order to consider various configurations of the stirrer, the revolution angular velocity ω, the diameter D of the reaction vessel, the distance L between the rotation center and the bottom of the reaction vessel, the installation angle θ of the reaction vessel with respect to the revolution rotation axis, the liquid volume V of the reaction solution The analysis was performed using the density ρ of the reaction solution and the surface tension σ of the reaction solution as variables.

  Further, FIG. 6 shows the analysis results regarding the evaluated evaluation variable, the inclination angle parameter α, and the capillary parameter χ. FIG. 6 plots the tilt angle parameter α and the capillary parameter χ, and shows the liquid surface shape of the reaction solution obtained by numerical analysis. The plot filled in black in the graph was judged to be sufficiently inclined to expose the bottom of the container. From this result, it can be seen that the region surrounded by the broken line, that is, the inclination angle parameter α and the capillary parameter χ must satisfy the following conditions as a condition for efficient stirring.

[Formula 10]
0.09 <χ <0.135 and α <1
By designing the shape and configuration of the reaction vessel agitator 2 and the reaction vessel so as to satisfy the tilt angle parameter α and the capillary parameter χ as described above, the liquid of the reaction solution can be obtained at any liquid volume. The surface can be inclined to the extent that the bottom surface of the container is exposed, and the reaction liquid with a low liquid volume can be efficiently stirred.

  Specifically, an example in which each parameter is examined in order to sufficiently stir a low-volume reaction solution will be described in Example 2. In this example, it is assumed that a reaction liquid of 100 μL or less is stirred using the reaction vessel stirring device 2 as a low liquid volume.

  In the analyzer 100, the reaction solution 1 in the reaction vessel is sucked by the reaction solution suction nozzle 115 and sent to the detection unit 116. In order to aspirate reliably with the reaction liquid suction nozzle 115, the reaction liquid 1 accommodated in the reaction container needs to have a certain level of liquid level (for example, the level of the liquid level from the bottom is 15 mm or more). In order to obtain a sufficient liquid level while accommodating 100 μL of the reaction solution, the diameter D of the reaction vessel needs to be small, for example, the diameter D needs to be 3 mm or less.

Moreover, the general surface tension of the reaction liquid 1 is 0.04 N / m, and is about 0.08 N / m at the maximum. The density ρ of the reaction solution was assumed to be 1000 kg / m ³ .

  Further, the distance L between the rotation center and the container bottom is set to 20 mm or more, and the installation angle θ is set to 10 °. If L and θ are excessively large, the size of the stirring device may be increased. Therefore, in order to make the size of the device compact, it is preferable to make the size of the conventional device about the same.

Under the above conditions, the rotational speed ω of rotation / revolution stirring is required to be 3500 min ⁻¹ or more in order to set the capillary parameter χ <0.135. By setting such design parameters, the liquid level of the reaction liquid can be inclined to the extent that the bottom surface of the container is exposed, and the low-volume reaction liquid can be sufficiently stirred.

  Next, an embodiment relating to an agitation apparatus that realizes optimum agitation conditions by setting agitation parameters for each reagent will be described.

  In the biochemical / immunological analyzer, the physical properties of the reagent, that is, the density ρ and the surface tension σ can be examined in advance and stored in the control unit of the analyzer. On the other hand, blood samples can also be predicted to some extent in advance and do not differ greatly. From these facts, the density ρ and surface tension σ of the reaction solution can be known in advance. The conditions of the reaction vessel and the stirring device, that is, the diameter D of the reaction vessel, the installation angle θ of the reaction vessel with respect to the revolution rotation axis, and the distance L between the rotation center and the bottom surface of the reaction vessel are determined in advance.

  Therefore, by setting [Equation 10] as an index for each analysis item in advance and setting and registering the rotation and revolution speed ω by the reaction vessel agitator 2, it is possible to shorten the time as a biochemical / immunological analyzer, A more excellent effect can be exhibited.

  For example, as shown in FIG. 7, the storage device in the control unit 119 that controls the operation of the reaction vessel stirring device 2 has a database that stores the stirring parameters such as the rotational speed ω in association with each analysis item. When the analysis apparatus 100 performs the stirring by the reaction vessel stirring apparatus 2, the stirring parameter of the reagent associated with the analysis item requested for the reaction liquid in the reaction vessel is read from the database, and the optimal stirring condition for the analysis item is obtained. Can be adjusted. According to this example, the stirring conditions can be optimally adjusted with a reagent that is difficult to mix (a reagent with a large density ρ and surface tension σ) and a reagent that is not, so that sufficient stirring can be achieved even when the amount of liquid is small, For analysis items that are easily mixed, the time required for the stirring process can be shortened.

  In addition, an input screen for inputting the conditions of each reagent in the database in advance can be displayed on the display device 121 such as a display, and the operator can set the reagent conditions or the stirring conditions for each reagent by an input means such as a keyboard or a touch panel. You may comprise as follows.

  Further, when the amount of the reaction solution varies depending on the analysis item, the amount of the reaction solution is stored for each analysis item, and the reaction contained in the reaction vessel at the time of stirring by the reaction vessel stirring device 2 It is also possible to control so as to achieve an optimum stirring operation according to the amount of liquid 1.

  In this embodiment, only the rotation speed is set as a change parameter. However, the installation angle θ of the reaction vessel with respect to the revolution rotation axis, the diameter D of the reaction vessel, the liquid volume V of the reaction solution, etc. are used as change parameters [Equation 10 It is also possible to carry out the stirring step under the optimum conditions.

For example, by making the installation angle θ of the reaction vessel adjustable, the stirring step can be performed under optimum conditions.

DESCRIPTION OF SYMBOLS 1 Reaction liquid 2 Stirring device 3 Rotation drive generation part 4 Container holding part 5 Revolving rotation part 6 Bearing 7 Rotation control member 100 Analyzer 101 Rack 102 Sample container 103 Sample dispensing nozzle 104 Incubator disk 105 Reaction container 106 Sample dispensing tip and Reaction vessel agitation and transport mechanism 107 Sample dispensing tip and reaction vessel holding member 109 Sample dispensing tip and reaction vessel disposal hole 110 Sample dispensing tip mounting position 111 Reagent disc 112 Reagent disc cover opening 114 Reagent dispensing nozzle 115 Reactive solution suction Nozzle 116 Detection unit 117 Rack transport line 118 Reagent container 119 Control unit 120 Database

Claims (9)

A container stirring device for stirring the liquid contained in the container by rotating and revolving the container, and
A suction nozzle for sucking the liquid contained in the container;
An analyzer for analyzing the liquid sucked by the suction nozzle;
In the automatic analyzer comprising the controller for controlling the operation of the container agitator, the suction nozzle and the analyzer,
The container agitator is
A container holding part capable of holding a container having a diameter of 3.0 mm or less and capable of containing a liquid of 100 uL or less,
The revolution angular velocity is ω [rad / s], the diameter of the container is D [m], the distance L [m] between the rotation center and the bottom surface of the container, the installation angle of the container with respect to the revolution rotation axis is θ [rad], and the liquid liquid When the amount is V [m ³ ], the density of the liquid is ρ [kg / m ³ ], the surface tension of the liquid is σ [N / m], and the acceleration of gravity is g [m / s ² ],
As a parameter representing the angle of the liquid level, the value of tan ⁻¹ (8V / πD ³ ) / tan ⁻¹ ((ω ² Lcos θ + g) sin θ / (− (ω ² Lsin ² θ + gcos θ))) is greater than 0.09 and 0 .135 a smaller, and,
As a parameter representing the influence of surface tension, the value of ((σ / (ρ (ω ⁴ L ² sin ² θ + ⁴ gω ² Lsin ² θ cos θ + g ² ) ^ (1/2))) ^ (1/2)) / D is 1 An automatic analyzer characterized by being smaller . The automatic analyzer according to claim 1, wherein
A revolution rotating part for relatively rotatably holding the container holding part;
A rotation drive unit that rotationally drives the entire revolution rotation unit;
An automatic analyzer comprising: a rotation suppressing member that suppresses rotation of the container holding portion with respect to rotational drive of the revolution rotation. The automatic analyzer according to claim 2,
The automatic analyzer according to claim 1, wherein the container holding part is fixed to the revolution rotating part via a bearing. The automatic analyzer according to claim 1, wherein
An automatic analyzer characterized in that the rotation speed and the rotation speed of the container stirring device are the same and the rotation directions are opposite. The automatic analyzer according to claim 4,
The automatic analyzer is characterized in that the rotational speed is 3500 min ^-1 or more. The automatic analyzer according to claim 1, wherein
The container is a reaction container containing a reaction solution,
A storage device is provided for associating and storing the stirring conditions of the container stirring device for each analysis item
The control device sets an agitation condition of the container agitator based on an agitation condition set for an analysis item requested for the reaction liquid stored in the reaction vessel. apparatus. The automatic analyzer according to claim 6,
An automatic analyzer characterized in that the stirring condition is a revolution angular velocity. In the method of stirring the liquid contained in the container by rotating and revolving the container,
The revolution angular velocity is ω [rad / s], the diameter of the reaction vessel is D [m], the distance L [m] between the rotation center and the bottom surface of the reaction vessel, the installation angle of the reaction vessel with respect to the revolution rotation axis is θ [rad], The liquid volume of the reaction liquid is V [m ³ ], the density of the reaction liquid is ρ [kg / m ³ ], the surface tension of the reaction liquid is σ [N / m], and the gravitational acceleration is g [m / s ² ]. sometimes,
Diameter D of the vessel 3. When 0 mm or less and the volume V of the reaction solution is 100 uL or less,
As a parameter representing the angle of the liquid level, the value of tan ⁻¹ (8V / πD ³ ) / tan ⁻¹ ((ω ² Lcos θ + g) sin θ / (− (ω ² Lsin ² θ + gcos θ))) is greater than 0.09 and 0 .135 a smaller, and,
As a parameter representing the influence of surface tension, the value of ((σ / (ρ (ω ⁴ L ² sin ² θ + ⁴ gω ² Lsin ² θ cos θ + g ² ) ^ (1/2))) ^ (1/2)) / D is 1 A stirring method characterized by being smaller . In the stirring device that stirs the liquid contained in the container by rotating and revolving the container,
The revolution angular velocity is ω [rad / s], the diameter of the reaction vessel is D [m], the distance L [m] between the rotation center and the bottom surface of the reaction vessel, the installation angle of the reaction vessel with respect to the revolution rotation axis is θ [rad], The liquid volume of the reaction liquid is V [m ³ ], the density of the reaction liquid is ρ [kg / m ³ ], the surface tension of the reaction liquid is σ [N / m], and the gravitational acceleration is g [m / s ² ]. sometimes,
The container diameter D is 3 . When 0 mm or less and the volume V of the reaction solution is 100 uL or less,
As a parameter representing the angle of the liquid level, the value of tan ⁻¹ (8V / πD ³ ) / tan ⁻¹ ((ω ² Lcos θ + g) sin θ / (− (ω ² Lsin ² θ + gcos θ))) is greater than 0.09 and 0 .135 a smaller, and,
As a parameter representing the influence of surface tension, the value of ((σ / (ρ (ω ⁴ L ² sin ² θ + ⁴ gω ² Lsin ² θ cos θ + g ² ) ^ (1/2))) ^ (1/2)) / D is 1 A stirrer characterized by being smaller .