JP6338766B2 - 非ニュートン流体とニュートン流体の並行層流を利用したマイクロ粒子分離及び洗浄方法 - Google Patents
非ニュートン流体とニュートン流体の並行層流を利用したマイクロ粒子分離及び洗浄方法 Download PDFInfo
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Description
前述したストークス抗力FDは、下記式4で表すことができる。
実験例1では、粒子が流線を横切って移動する比率が非ニュートン流体内に含まれているDNA濃度によって異なることを示すための実験を行った。
非ニュートン流体の流動内に属した粒子の流線を横切る方向への移動速度は有効直径の二乗に比例する(式5参照)。
本実験例2では、非ニュートン流体内に含まれている直径9.9μmのポリスチレン(PS;Polydstyrene)マイクロ粒子(球状)を、同じ流体内の直径2.0μmの粒子から分離してニュートン流体内に移す実験を行った。
10 流体チャネル
11 第1入口
12 第2入口
15 第1出口
16 第2出口
Claims (16)
- 流体チャネル内に非ニュートン流体とニュートン流体を並行層流状に流動させて、前記流体チャネルの内部に作用する力の平衡をなす粒子集束位置の変化によって、前記非ニュートン流体内に含まれている微小粒子の中から目標粒子を前記ニュートン流体内へ分離または移動させることを特徴とする、非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
- 前記非ニュートン流体は、
緩和時間が十分に大きいポリマーを含有して、前記目標粒子に対する弾性揚力が慣性揚力よりもさらに大きく、
前記ポリマー含有濃度が高いため、前記目標粒子の流線を横切る移動速度を十分に有するようにする、請求項1に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。 - 前記非ニュートン流体は、
血液、血球が粉砕された血液、血清またはリンパを含む天然生体液の中から選択された少なくとも一つからなる、請求項2に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。 - 前記非ニュートン流体は、人為的に粘弾性を持つように人工ポリマーが加えられて作成される、請求項2に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
- 前記人工ポリマーが、
緩和時間が1ms以上の水溶性ポリマーを含む、請求項4に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。 - 前記人工ポリマーが、
λ−DNA、ペアレント・エッセンシャル・オイル及びポリビニルピロリドンの中から選択された少なくとも一つからなる、請求項5に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。 - 前記ニュートン流体は、前記目標粒子に対する慣性揚力が弾性揚力よりもさらに大きい流体からなる、請求項2に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
- 前記ニュートン流体は、水、グリセリン水溶液またはリン酸緩衝生理食塩水を含む、請求項7に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
- 前記流体チャネル内の前記非ニュートン流体と前記ニュートン流体がそれぞれ占める断面積の面積比が約1:1である、請求項1に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
- 前記流体チャネルの大きさが前記目標粒子の有効直径に比例して形成される、請求項1に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
- 前記流体チャネルの断面積に対するアスペクト比が1以上である、請求項10に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
- 前記流体チャネルの長さが、前記非ニュートン流体の流速または弾性数に反比例して形成される、請求項10に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
- 前記流体チャネルの長さが1L〜10Lの範囲以内で形成され、
ここで、前記流体チャネルの最小長さLが、
を満足する(式中、Umaxは最大チャネル速度であり、fLは経験比例定数であり、wは流体チャネルの幅であり、dは目標粒子の有効径であり、μは流体の粘度であり、ρは流体の密度である。)、請求項12に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。 - 前記流体チャネルの最小長さL以内で慣性集束がなされるための最小流量Qが、
を満足する(式中、hは前記流体チャネルの高さであり、wは流体チャネルの幅であり、fLは経験比例定数である)、請求項13に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。 - 前記流体チャネルが流体チップの内部に形成され、
前記流体チップがシリコーン系ポリマー、プラスティック、ガラス、シリコン及び金属の中から選択されるいずれか一つからなる、請求項1に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。 - 前記流体チップが、前記ニュートン流体の流入する第1入口と前記非ニュートン流体の流入する第2入口とが所定の角度を成して前記流体チャネルの先端部に互いに合岐されるように形成され、
前記流体チャネルの後端部から、前記目標粒子の含まれた前記ニュートン流体を排出する第1出口と前記非ニュートン流体を排出する第2出口とが所定の角度を成して分岐されて形成され、
前記第1出口及び第2出口の流動抵抗が、流路の断面設計変更またはバルブ活用を介して、前記非ニュートン流体と前記ニュートン流体が分かれる流線の位置を調節する、請求項15に記載の非ニュートン流体とニュートン流体の並行層流を利用した微小粒子分離及び洗浄方法。
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