JP6334251B2 - Genetic testing equipment - Google Patents

Description

  The present invention relates to a genetic test apparatus using a DNA microarray (also called a DNA chip).

  Advances in genome analysis have elucidated biological molecules involved in the physiological responses of various organisms. These bio-related molecules include DNA, proteins, sugar chains, cells, etc. Those whose functions and structures have been elucidated are used for various industrial applications such as drug discovery, clinical testing, food testing, and environmental testing. .

  In tests such as clinical tests, the following methods are commonly used. That is, when a specimen is brought into contact with a device in which a probe molecule (hereinafter referred to as a ligand) that specifically binds to a biological molecule to be detected (hereinafter referred to as an analyte) is immobilized on a carrier, the analyte is attached to the specimen. Is present, it binds to the ligand and the analyte is captured on the carrier, so that the captured analyte is detected.

  Even in the inspection methods as described above, in recent years, speeding up and automation have been demanded, and a detection method for comprehensively measuring hundreds to tens of thousands of biologically relevant molecules simultaneously has been demanded. Device design using integration technology, so-called MEMS technology, is possible, and so-called microarrays are used for comprehensive analysis in drug discovery research and biotechnology research. In particular, genetic testing using a so-called DNA microarray (also called a DNA chip) in which nucleic acid molecules are immobilized on a carrier has been put into practical use.

  In analysis using a DNA microarray, first, for example, a sample DNA that has been previously fluorescently labeled with a fluorescent substance is brought into contact with the DNA microarray, and the probe DNA immobilized on the carrier and the sample DNA are specifically hybridized. Let Thereafter, the DNA microarray is washed, and the sample DNA is identified or quantified by detecting and measuring the fluorescent signal emitted from the fluorescent substance.

  A DNA microarray usually has a size like a glass slide, and a sample is suspended on it and covered with a preparation. In the future, it will be necessary to automatically process a large number of microarrays depending on the application. In that case, miniaturization of the microarray is desired, and development of an efficient means for automatically processing the miniaturized microarray is desired.

  An example of a genetic testing apparatus using a DNA microarray is Patent Document 1. The genetic testing device disclosed in Patent Document 1 includes a temperature adjustment unit for a DNA microarray, a detection unit that detects an optical signal from the DNA microarray, a fluid transport unit that supplies and discharges fluid to and from the DNA microarray, and an overall operation. A control unit for controlling is provided.

  For example, as shown in FIG. 13, the genetic testing apparatus includes a DNA microarray 101 and a carrier support member 102 that fixes the DNA microarray 101, and the DNA microarray 101 fixed to the carrier support is movable. In addition, as shown in FIG. 13, the genetic test apparatus includes a nucleic acid amplification reaction unit 103 that amplifies a predetermined region by a polymerase chain reaction (PCR) or the like as a nucleic acid extracted from a test target organism, and a nucleic acid amplification reaction such as PCR. A hybridization reaction unit 104 that performs a hybridization reaction using the later solution, a cleaning unit 105 that cleans the DNA microarray 101 after the hybridization reaction, and a detection unit 106 that detects fluorescence from the DNA microarray 101 after the cleaning Prepare.

JP 2004-28775 A

  However, in the genetic test apparatus, for example, when a series of processes such as a hybridization reaction process, a washing process, and a detection process are automated, a configuration for performing each process is required and the size is increased. was there.

  Therefore, in view of the above-described circumstances, an object of the present invention is to provide a genetic test apparatus that can automate various processes using a DNA microarray and can be miniaturized.

The present invention that has achieved the above-described object includes the following.
(1) A DNA microarray can be mounted, a chip driver for positioning the DNA microarray at a predetermined position, a tube into which a reaction solution after a nucleic acid amplification reaction can be placed, and a hollow container attached to the tube A reaction part having a mechanism for supplying the solution to the tube, a temperature adjustment part for adjusting the temperature of the solution in the tube, and the DNA in a state positioned at the first position in the hollow container by the chip driving part. A cleaning liquid supply means for supplying a cleaning liquid to the microarray; and a detection section for detecting an optical signal from the DNA microarray in a state positioned at the second position in the hollow container by the chip driving section. A gene for producing a hybridization reaction solution by supplying the solution to the reaction solution in the tube査 apparatus.
(2) The mechanism is a solution holding material having at least an end face made of a film and holding a solution therein. The solution holding material is placed on the opening end of the tube and the solution is held by breaking the film. The genetic test apparatus according to (1), wherein the solution in the material is supplied into the tube.
(3) The chip driving unit can attach a carrier support member to which the DNA microarray is attached, and the film is broken by driving the carrier support member to press the film. Genetic testing equipment.
(4) The gene according to (1), wherein the hollow container has an opening at the first position, and the cleaning liquid supply means supplies the cleaning liquid to the DNA microarray through the opening. Inspection device.
(5) The genetic test apparatus according to (1), wherein at least the second position of the hollow container has translucency.
(6) The genetic test apparatus according to (1), wherein the reaction unit has a stirring means for stirring the solution in the tube.
(7) The temperature adjusting unit performs temperature control related to a hybridization reaction using the DNA microarray and temperature control related to a nucleic acid amplification reaction performed in the tube. .

  The genetic testing apparatus according to the present invention can realize genetic testing using a DNA microarray without further human work. Therefore, the genetic testing device according to the present invention can be reduced in cost.

It is a schematic block diagram of the genetic testing apparatus to which this invention is applied. It is a block diagram which shows the control structure of the genetic test apparatus to which this invention is applied. It is a principal part perspective view which shows the chip drive part and reaction part in a genetic test | inspection apparatus. It is principal part sectional drawing which shows the process of the hybridization reaction in a reaction part. It is principal part sectional drawing which shows the process of adjusting the solution for hybridization reaction in a reaction part. It is principal part sectional drawing which shows the stirring means in the reaction part. It is principal part sectional drawing which shows the state which put the solution for hybridization reaction in the tube. It is principal part sectional drawing which shows the state which immersed the chip | tip in the solution for hybridization reaction put into the tube. It is principal part sectional drawing which shows the state which positioned the chip | tip in the washing | cleaning process after hybridization reaction. It is principal part sectional drawing which shows the state which positioned the chip | tip in the detection process after a washing | cleaning process. It is principal part sectional drawing which shows the other form of the washing | cleaning process after hybridization reaction. It is principal part sectional drawing which shows the further another form of the washing | cleaning process after hybridization reaction. It is a schematic block diagram of the conventional genetic testing apparatus.

  A genetic testing apparatus to which the present invention is applied performs genetic testing using a DNA microarray. The DNA microarray that can be used in the genetic test apparatus is not particularly limited, and examples thereof include those composed of a flat carrier and a DNA fragment (referred to as a probe) immobilized on the carrier surface.

  As shown in FIG. 1, the genetic test apparatus 1 includes at least a chip drive unit 2 for mounting a DNA microarray and positioning the DNA microarray at a predetermined position, a hybridization reaction process using the DNA microarray, and washing. The reaction part 3 which can perform a process and a detection process, and the detection part 4 for detecting the optical signal from a DNA microarray are provided. Moreover, the gene test | inspection apparatus 1 has the control part 5 which controls operation | movement of the whole apparatus, as shown in FIG. The control unit 5 performs drive control of the chip drive unit 2, operation control of the temperature adjustment unit 6 that adjusts the temperature in the reaction unit 3, and detection operation control of the detection unit 4.

  As shown in FIG. 3, the chip drive unit 2 includes a guide member 8 having a guide 7 in a predetermined uniaxial direction, and a uniaxial actuator 9 slidably attached to the guide 7 of the guide member 8. The tip 10 attached to the tip of 9 can be freely moved in the axial direction of the guide 7 (in the direction of arrow A in FIG. 3) and in the axial direction of the uniaxial actuator 9 (in the direction of arrow B in FIG. 3).

  The chip 10 includes a DNA microarray 11 and a carrier support member 12 to which the DNA microarray 11 is attached. The shape and configuration of the chip 10 are not limited at all, but as an example, a carrier support member to which a carrier is fixed disclosed in Japanese Patent No. 4950336 can be applied. That is, the carrier support member 12 has a concave portion formed on the side surface in a substantially conical shape, and the DNA microarray 11 is attached to the bottom surface of the concave portion. The concave portion of the carrier support member 12 is formed by a bottom surface to which the DNA microarray is attached and an inclined surface located in the direction of arrow B in FIG.

  Further, the chip 10 is detachable from the distal end portion of the uniaxial actuator 9. For example, one end of the chip 10 is open, and the tip 10 can be attached to the uniaxial actuator 9 by press-fitting the tip of the uniaxial actuator 9 into the opening.

  In the reaction unit 3, at least the hybridization reaction using the DNA microarray 11 and the washing of the DNA microarray 11 after the hybridization reaction are performed. As an example, as shown in FIG. 3, the reaction unit 3 includes a tube 13 that holds a solution used for the hybridization reaction, and a hollow container 14 that is attached to the open end of the tube 13. Although not shown in FIG. 3, the reaction unit 3 includes a temperature adjustment unit 6 that adjusts the solution temperature in the tube 13 to a predetermined temperature. Moreover, although the reaction part 3 is not shown in FIG. 3, it has a stirring means for stirring the solution in the tube 13.

  As shown in FIG. 3, the hollow container 14 has an open end opposite to the end to which the tube 13 is attached, and the tip drive unit 2 is inserted so that the shaft of the uniaxial actuator 9 to which the tip 10 is attached is inserted. Attached to. With the hollow container 14 and the chip drive unit 2 connected, the chip 10 can be positioned in the hollow container 14 in the direction of arrow B.

  The hollow container 14 has a window 15 having translucency in a part of the side surface. Although not shown in FIG. 3, the window 15 faces the detection unit 4. That is, the detection unit 4 detects an optical signal from the DNA microarray 11 through the window 15. In addition, when the hollow container 14 is formed from the material which has translucency, of course, it is not necessary to have the window 15. FIG.

  The hollow container 14 has an opening 16 in a part of the side surface. Although the opening 16 is located below the window 15 in FIG. 3, the opening 16 is not limited to this and may be formed at any position on the side surface. For example, the opening 16 may be formed at the same height as the window 15 and at a position adjacent to the window 15 or a position opposite to the window 15. Although not shown, a pipe tip for supplying a solution such as a cleaning liquid is inserted into the opening 16. The pipe inserted into the opening 16 can supply a solution such as a cleaning liquid to the chip 10 located inside the hollow container 14 at a predetermined timing. Note that a pipe tip for supplying a gas such as argon gas or air may be inserted into the opening 16.

  In the genetic testing apparatus configured as described above, as shown in FIG. 4A, first, a solution (nucleic acid amplification reaction solution) containing a nucleic acid amplified by a nucleic acid amplification reaction such as a polymerase amplification reaction (PCR) is used. Prepare in the tube 13.

  And as shown in FIG.4 (b), it is set as the solution of a composition suitable for hybridization reaction by adding the solution of a predetermined composition to this nucleic acid amplification reaction solution. The solution having a composition suitable for the hybridization reaction means a reaction in which specific hybridization is formed between the probe of the DNA microarray 11 and the nucleic acid fragment obtained by the nucleic acid amplification reaction. In the hybridization reaction, the stringency is defined by conditions such as salt concentration, organic solvent concentration and temperature in the solution. By performing the hybridization reaction under highly stringent conditions, non-specific hybridization is prevented and specific hybridization is formed. For example, highly stringent conditions can be achieved by lowering the salt concentration in the solution, increasing the organic solvent concentration, or raising the reaction temperature.

  Under the highly stringent conditions as described above, specific hybridization is formed between the probe of the DNA microarray 11 and the nucleic acid fragment obtained by the nucleic acid amplification reaction. Specific hybridization means that hybridization can be performed when the degree of identity between sequences is 80% or more, preferably 90% or more, more preferably 95% or more, and more preferably 98% or more.

  Then, after the solution in the tube 13 becomes a solution having a composition suitable for the hybridization reaction, as shown in FIG. 4C, the chip driving unit 2 is lowered, and the DNA microarray 11 is placed in the solution in the tube 13. Soak. At this time, the temperature adjusting unit 6 sets the solution in the tube 13 to a temperature suitable for the hybridization reaction (high stringent condition).

  Here, the DNA microarray 11 is attached to the recess of the carrier support member 12. Therefore, when the chip drive unit 2 is lowered and the DNA microarray 11 is immersed in the solution in the tube 13, the inclined surface forming the concave portion makes it easier for bubbles to escape upward. If the bubbles do not escape well, the contact between the DNA microarray 11 and the solution is hindered, and the hybridization reaction may be hindered. By adopting the above-described configuration that allows air bubbles to escape well, good contact between the DNA microarray 11 and the solution can be achieved. In other words, the amount of solution added to the nucleic acid amplification reaction solution is sufficient to immerse the entire DNA microarray 11 in the solution in the tube 13 as shown in FIG.

  By the way, as shown in FIG. 4B, when a solution of a predetermined composition is added to the nucleic acid amplification reaction solution to obtain a hybridization reaction solution, for example, a method as shown in FIG. 5 can be employed. In other words, a solution holding material 21 having upper and lower end surfaces made of a film 20 and holding the solution therein is placed on the open end of the tube 13 containing the nucleic acid amplification reaction solution, and the chip driving unit 2 is lowered to support the carrier. In this method, the film 20 on the upper and lower end surfaces is split at the tip of the member 12 and the solution inside the solution holding material 21 is supplied into the tube 13.

  The solution holding material 21 can be produced, for example, by pasting the film 20 on one end surface of a resin cylindrical member, filling the solution, and then pasting the film 20 on the other end surface. The film 20 and the cylindrical member can be bonded by means such as thermocompression bonding. The solution holding material 21 may be made entirely from a film. Further, the solution filled in the solution holding material 21 has a composition that is mixed with the nucleic acid amplification reaction solution to become a hybridization reaction solution. At this time, if the amount of the nucleic acid amplification reaction solution is very small and the composition change can be ignored even when mixed, the solution holding material 21 may be filled with the hybridization reaction solution itself.

  In the genetic test apparatus 1, since the nucleic acid amplification reaction solution can be made into a hybridization reaction solution by the mechanism as shown in FIG. 5, a flow path such as a pump or a valve for adding the solution to the nucleic acid amplification reaction solution No mechanism is required. For this reason, the genetic test apparatus 1 having a mechanism as shown in FIG. 5 can simplify the apparatus configuration and realize downsizing.

  By the way, the genetic test apparatus 1 may perform a nucleic acid amplification reaction in the reaction unit 3 and then perform a hybridization reaction using a solution after the nucleic acid amplification reaction. The nucleic acid amplification reaction is not particularly limited, and any principle such as a PCR method or a LAMP method may be adopted. When performing a nucleic acid amplification reaction using the PCR method in the reaction unit 3, for example, first, nucleic acids extracted from cells or tissues and reagents (substrate, primers, enzymes such as polymerase) necessary for the nucleic acid amplification reaction are put in the tube 13. Fill. And the nucleic acid amplification reaction can be performed in the tube 13 by controlling the solution temperature in the tube 13 according to the temperature cycle which the temperature adjustment part 6 preset. Specifically, the temperature adjustment unit 6 can set, for example, 94 ° C. as the heat denaturation temperature, set, for example, 55 ° C. as the primer annealing temperature, and set, for example, 72 ° C. as the extension reaction temperature by the DNA polymerase. And the temperature control part 6 can be set so that it may carry out, for example, 25-30 cycles by making heat denaturation temperature, annealing temperature, and elongation reaction temperature into 1 cycle.

  After completion of the nucleic acid amplification reaction, as shown in FIGS. 4 and 5, a solution having a predetermined composition can be added to the tube 13 to obtain a hybridization reaction solution. In the genetic test apparatus 1, the nucleic acid amplification reaction solution and the hybridization reaction solution are used in the tube 13 regardless of whether the nucleic acid amplification reaction is performed in the reaction unit 3 or the nucleic acid amplification reaction is performed in another apparatus. Therefore, it is preferable to sufficiently mix a solution having a predetermined composition. For example, the solution in the tube 13 can be agitated by the vertical movement of the chip 10 by moving the chip driving unit 2 to which the chip 10 is attached up and down. In addition, the genetic testing device 1 can include a stirring unit for stirring the solution in the tube 13.

  As a stirring means, as shown to Fig.6 (a), it can be set as the mechanism which consists of the stirring blade 25 and the axis | shaft 26 attached to the stirring blade 25. FIG. By rotating the shaft 26 with a motor (not shown), the stirring blade 25 can stir the solution.

  Moreover, as a stirring means, as shown in FIG.6 (b), it can be set as the mechanism which consists of the stirring element 27 which consists of a magnetic body, and the stirrer main body 28 which has a magnetic body which can be rotated inside. By rotating the magnetic body inside the stirrer main body 28, the stirrer 27 in the tube 13 is rotated, whereby the solution can be stirred.

  Further, as the stirring means, as shown in FIG. 6C, a mechanism including a pipe 29 for introducing a gas such as argon gas or nitrogen and a bubble generating unit 30 connected to the pipe 29 can be used. By generating bubbles from the bubble generation unit 30 via the pipe 29, the solution in the tube 13 can be stirred.

  Furthermore, as the stirring means, as shown in FIG. 6D, a mechanism including a pipette device 31 and a tip 32 attached to the tip of the pipette device 31 can be used. The pipette device 31 repeats the suction and discharge of the solution with the tip 32 of the tip 32 positioned in the solution in the tube 13, whereby the solution in the tube 13 can be stirred.

  Furthermore, as a stirring means, as shown in FIG.6 (e), it can be set as the mechanism which consists of the vibration generator 33 which generate | occur | produces vibrations, such as a vibration by rotation, and an ultrasonic wave. By transmitting the vibration generated from the vibration generator 33 into the solution in the tube 13, the solution in the tube 13 can be stirred.

  In addition, as a stirring means, it can also be used combining the mechanism shown to Fig.6 (a)-(e).

  As described above, the reaction unit 3 performs the hybridization reaction using the DNA microarray 11. That is, the reaction unit 3 of the genetic test apparatus 1 is configured so that the chip 10 is placed in the tube 13 as shown in FIG. 8 from the state in which the solution for the hybridization reaction is put in the tube 13 as shown in FIG. It will be in the state immersed in. Next, the genetic test apparatus 1 performs a cleaning process of the chip 10 and a detection process of optical signals generated from the DNA microarray 11.

  In the washing step, first, as shown in FIG. 9, the chip driving unit 2 is driven to a position where the DNA microarray 11 faces the opening 16 (first position). Although not shown, a cleaning liquid is supplied to the chip 10 through the opening 16. The cleaning liquid supplied to the chip 10 is stored in the tube 13 after the chip 10 including the DNA microarray 11 is cleaned.

  Here, the washing solution is a solution in which specific hybridization between the probe fixed to the DNA microarray 11 and the nucleic acid fragment obtained by the nucleic acid amplification reaction is maintained, and the nonspecifically hybridized nucleic acid fragment can be washed away. It is. In addition, by supplying a cleaning solution to the chip 10, nucleic acid fragments and various components attached to the DNA microarray 11 can be washed away.

  As the cleaning solution, a solution having the same composition as the solution for the hybridization reaction can be used. Specifically, for example, a solution of 42 ° C., 5 × SSC, 0.1% SDS can be used. Further, a solution of 50 ° C., 5 × SSC, 0.1% SDS can be preferably used. More preferably, a solution of 65 ° C., 0.1 × SSC and 0.1% SDS can be used.

  In the optical signal detection process generated from the DNA microarray 11 after the cleaning process, as shown in FIG. 10, the chip driver 2 is driven, and the position where the DNA microarray 11 faces the window 15 (second position) To do. And although not shown in figure, the detection part 4 detects the optical signal which arises from a DNA microarray through the window 15. FIG. Specifically, when the nucleic acid fragment amplified in the nucleic acid amplification reaction is labeled with a fluorescent substance, the detection unit 4 irradiates the DNA microarray 11 with excitation light, and the detection unit 4 detects the excited fluorescence. . In addition, when the hollow container 14 consists of a material which has translucency, a detection process can be performed in the arbitrary positions of the hollow container 14 (2nd position).

  More specifically, it is preferable to use an imaging optical system detector as the detection unit 4. The detector of the imaging optical system irradiates the carrier with excitation light and detects the intensity of the fluorescence obtained. The detector of the imaging optical system usually includes a laser for irradiating excitation light, a fluorescent filter that transmits only fluorescence of a target wavelength, and a light detection unit (for example, a CCD) that detects fluorescence transmitted through the fluorescent filter. Camera). Usually, the entire DNA microarray 11 is irradiated obliquely with excitation light at a time, and fluorescence is detected from the front of the DNA microarray 11. Irradiating the excitation light obliquely with respect to the DNA microarray 11 means that the angle between the surface of the DNA microarray 11 and the laser beam is less than 90 °, and usually 30 to 70 °, preferably 40 to 60 °. Irradiate at an angle of. In the detection unit 4 of the imaging optical system, it is not necessary to scan the entire surface of the DNA microarray 11 with a laser, and detection can be performed in a short time. In order to irradiate the entire support with excitation light at once, the DNA microarray 11 may be a relatively small size, for example, a dimension of 10 mm or less, preferably 5 mm or less, more preferably 3 mm or less, most preferably 1 to 5 mm square. The size of

  By the way, the above-described cleaning process supplies the cleaning liquid to the chip 10 through the opening 16. However, the method for supplying the cleaning liquid is not particularly limited. For example, as shown in FIG. 11, the cleaning liquid supply attached to the chip driving unit 2 so that the tip portion faces the DNA microarray 11 attached to the chip 10. The tube 40 may be used. By using the cleaning liquid supply tube 40, it is not necessary to form the opening 16 in the hollow container 14, and the hollow container 14 can have a simple configuration. In this case, the cleaning step can be performed at an arbitrary position in the hollow container 14 (first position).

  As a method for supplying the cleaning liquid, for example, as shown in FIG. 12, the cleaning liquid is disposed along the inner wall of the hollow container 14 so that the tip portion faces the DNA microarray 11 attached to the chip 10. A cleaning liquid supply pipe 50 may be used. By using the cleaning liquid supply pipe 50, it is not necessary to form the opening 16 in the hollow container 14, and the hollow container 14 can have a simple configuration. The cleaning liquid supply pipe 50 may be fixed to the hollow container 14 or detachable from the hollow container 14. In this case, the cleaning process is performed at a position facing the tip of the cleaning liquid supply pipe 50 (first position).

  As described above, in the genetic test apparatus 1 to which the present invention is applied, the reaction unit 3 can perform the hybridization reaction using the reaction solution after the nucleic acid amplification reaction, and then can perform the washing step in the reaction unit 3. . Therefore, in the genetic test apparatus 1 to which the present invention is applied, it is not necessary to prepare a mechanism for the hybridization reaction and a mechanism for the washing separately, and the apparatus configuration can be simplified and the size can be reduced. it can. In particular, since the nucleic acid amplification reaction can be performed in the reaction unit 3, in this case, a mechanism for the nucleic acid amplification reaction, a mechanism for the hybridization reaction, and a mechanism for washing are prepared separately. There is no need. That is, the genetic test apparatus 1 to which the present invention is applied can be downsized without complicating the apparatus configuration even when the nucleic acid amplification reaction is performed in the reaction unit 3.

DESCRIPTION OF SYMBOLS 1 ... Gene test apparatus, 2 ... Chip drive part, 3 ... Reaction part, 4 ... Detection part, 5 ... Control part, 6 ... Temperature adjustment part, 10 ... Chip, 11 ... DNA microarray, 13 ... Tube, 14 ... Hollow container

Claims (6)

A chip driver that can be equipped with a DNA microarray and positions the DNA microarray at a predetermined position;
A tube that can contain the reaction solution after the nucleic acid amplification reaction, a hollow container to which the open end of the tube is attached at the tip , a mechanism for supplying a solution to the tube, and a temperature of the solution in the tube are adjusted A reaction part having a temperature adjustment part;
A cleaning liquid supply means for supplying a cleaning liquid to the DNA microarray in a state positioned at the first position in the hollow container by the chip driver;
A detection unit that detects an optical signal from the DNA microarray positioned at the second position in the hollow container by the chip driving unit, and the mechanism is a reaction liquid in the tube attached to the hollow container A solution for hybridization reaction is supplied by supplying the solution to the above, and the hollow container has translucency at least in the second position .   The mechanism is a solution holding material having at least an end surface made of a film and holding the solution therein, and the solution holding material is placed on the opening end of the tube, and the film is divided to break the film in the solution holding material. The genetic test apparatus according to claim 1, wherein the solution is supplied into the tube.   The genetic test according to claim 2, wherein the chip driving unit can attach a carrier supporting member to which the DNA microarray is attached, and the film is divided by driving the carrier supporting member to press the film. apparatus.   The genetic test apparatus according to claim 1, wherein the hollow container has an opening at the first position, and the cleaning liquid supply means supplies the cleaning liquid to the DNA microarray through the opening.   The genetic test apparatus according to claim 1, wherein the reaction unit has a stirring means for stirring the solution in the tube.   The genetic test apparatus according to claim 1, wherein the temperature adjusting unit performs temperature control related to a hybridization reaction using the DNA microarray and temperature control related to a nucleic acid amplification reaction performed in the tube.

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