JP2009183244A - Simple device for quantitative determination by gene amplification - Google Patents
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本発明は、簡易に遺伝子増幅し、定量検出する装置および方法に関する。より詳しくは、反応容器の毛細管流路に導入した試料を、遺伝子増幅法により増幅反応を行いつつ、増幅度を蛍光色素を利用して定量検出を行なう装置および方法に関する。
The present invention relates to an apparatus and method for simple gene amplification and quantitative detection. More specifically, the present invention relates to an apparatus and method for performing quantitative detection using a fluorescent dye while performing an amplification reaction on a sample introduced into a capillary channel of a reaction vessel by a gene amplification method.
耐熱性ポリメラーゼを利用した遺伝子増幅法であるＰＣＲ法は、研究用の技術として開発され、遺伝子工学の技術発展に寄与するばかりではなく、生物のゲノム解析や創薬開発や、ウィルス感染検査などの臨床検査や、アレルギー物質の食品混入や、成分チェックの検査などにも適用されている。
The PCR method, which is a gene amplification method using a thermostable polymerase, was developed as a research technology, contributing not only to the advancement of genetic engineering technology, but also to analyzing the genome of organisms, developing drug discovery, testing for virus infection, etc. It is also applied to clinical tests, food contamination with allergens, and ingredient check tests.
また、ＬＡＭＰ法などＰＣＲ法以外の増幅原理に基づく遺伝子増幅法も開発されており、遺伝子増幅法が多様な目的に利用できるようになってきている。
In addition, gene amplification methods based on amplification principles other than the PCR method, such as the LAMP method, have been developed, and the gene amplification method can be used for various purposes.
また、検出対象の遺伝子の有無を検出する方法だけでなく、遺伝子増幅反応の増幅状態を解析しながら、対象とする遺伝子の存在数や濃度などのより多くの情報を得るリアルタイムＰＣＲ法なども開発されてきている。
In addition to detecting the presence or absence of the target gene, we also developed a real-time PCR method that obtains more information such as the number and concentration of the target gene while analyzing the amplification status of the gene amplification reaction. Has been.
通例は、検査などで用いるＰＣＲ法などの遺伝子増幅の実際の操作は、対象試料をプラスチック容器に入れて、その容器をヒートブロックなどの温度制御できる部分を持つ装置に設置して、温度を制御して、遺伝子の増幅反応を行い、反応後に、反応溶液を取り出して、アガロースゲルなどを用いたゲル電気泳動を行なって、エチジウムブロマイドなどのＤＮＡに結合する蛍光試薬を用いて、検出を行なう。
Usually, the actual operation of gene amplification such as PCR method used for testing, etc. puts the target sample in a plastic container and installs the container in a device with a temperature controllable part such as a heat block to control the temperature Then, a gene amplification reaction is performed, and after the reaction, the reaction solution is taken out, and gel electrophoresis using an agarose gel or the like is performed, and detection is performed using a fluorescent reagent that binds to DNA such as ethidium bromide.
遺伝子増幅の操作における対象試料をプラスチック容器に入れる作業や、反応終了後に、取り出して電気泳動を行なうための作業は、操作に熟練した作業者が、注意深く、少量の試料を取り扱って操作を行う必要があり、時間と手間、ひいては、費用を要する、簡易とはいえない、検査方法となっている。
The operation of placing the target sample in a plastic container for gene amplification and the operation of taking out and performing electrophoresis after the reaction is completed must be performed carefully by a skilled operator, handling a small amount of sample. It is an inspection method that is time-consuming, labor-intensive, and costly and not simple.
また、遺伝子増幅の操作後においては、反応に用いたプラスチック容器や、電気泳動に用いたアガロースゲルや、緩衝液などの廃棄物が多く出て、産業廃棄物として適正に処理を行う必要がある。
In addition, after gene amplification operations, a lot of waste such as plastic containers used for reactions, agarose gels used for electrophoresis, and buffer solutions are required, and it is necessary to properly treat them as industrial waste. .
半導体体加工における微細加工の手法を用いて、シリコンなどの板状の部材の片側表面に溝を構成させ、あるいは、片側表面からの貫通穴を構成し、板状部材を貼りあわせることにより液体試料の流路を形成して作成した反応容器を使った増幅分析方法（特許文献１）や反応装置（特許文献２）が開示されており、特殊な技術を持ち合わせていなくとも、遺伝子増幅反応操作が可能となってきているが、反応容器の作製操作が複雑であり、それゆえ、反応容器が特殊で高価であり、かつ、装置も高価である。
A liquid sample is formed by forming a groove on one side surface of a plate-like member such as silicon or forming a through-hole from one surface and bonding the plate-like member by using a fine processing technique in semiconductor body processing. An amplification analysis method (Patent Document 1) and a reaction apparatus (Patent Document 2) using a reaction vessel formed by forming a flow path of the above are disclosed, and a gene amplification reaction operation can be performed even without special technology. Although it has become possible, the operation of producing the reaction vessel is complicated, and therefore the reaction vessel is special and expensive, and the apparatus is also expensive.
遺伝子増幅により、より多くの情報を得るためのリアルタイムＰＣＲは、上記の人手での操作は難しいので、機械化されているが、そのための装置が高価であり、また、検出のための試薬は、高価な蛍光色素を用いる必要がある。
Real-time PCR for obtaining more information by gene amplification is difficult because it is difficult to perform the above-mentioned manual operation, but it is mechanized, but the equipment for that is expensive, and the reagent for detection is expensive. It is necessary to use an appropriate fluorescent dye.
リアルタイムＰＣＲにおいて、高価な蛍光色素が必要となる理由としては、安価なプラスチックやガラスを素材とする反応容器は、紫外線を通過させることができず、よって、アガロースゲル電気泳動で用いる、エチジウムブロマイドのような安価な試薬を用いることができないことがあげられる。
In real-time PCR, an expensive fluorescent dye is required because a reaction vessel made of an inexpensive plastic or glass cannot pass ultraviolet rays, and therefore, ethidium bromide used in agarose gel electrophoresis. Such an inexpensive reagent cannot be used.
紫外線を通過できる素材としては、石英があげられるが、石英の容器は、高価であり、かつ、使用目的にあうような形状に加工した容器を、大量に製造することは難しいとされていたが、近年、通信用の端末器の作製方法として、石英粉末を原料とし、金型で形状を付与で、内部貫通穴を容易に成型できる石英器の作製法が開示（特許文献３）されている。
簡易に遺伝子増幅操作ができ、かつ、その増幅遺伝子を簡易に、定量的に検出できる装置および方法を提供する。また、遺伝子増幅操作に伴う廃棄物処理の軽減に貢献する方法を提供する。
Provided are an apparatus and a method capable of easily performing gene amplification operation and easily and quantitatively detecting the amplified gene. In addition, the present invention provides a method that contributes to the reduction of waste treatment associated with gene amplification operations.
本発明は、蛍光検出ができる素材・構造を付与した反応容器の毛細管流路に、試料を流通させながら遺伝子増幅反応を行わせ、遺伝子増幅反応過程での遺伝子増幅の度合いを蛍光により検出して、遺伝子増幅程度を定量する装置であり、その装置を用いた方法である。
In the present invention, a gene amplification reaction is performed while circulating a sample in a capillary channel of a reaction vessel provided with a material / structure capable of detecting fluorescence, and the degree of gene amplification in the gene amplification reaction process is detected by fluorescence. An apparatus for quantifying the degree of gene amplification, and a method using the apparatus.
本発明は、増幅試料を、遺伝子増幅の反応溶液により、連続的または断続的に希釈して、反応容器の毛細管流路内に導入し増幅操作を行い、増幅度合いを検出する装置であり、その装置を用いた方法である。
The present invention is an apparatus for detecting the degree of amplification by amplifying an amplification sample by continuously or intermittently diluting it with a gene amplification reaction solution and introducing it into a capillary channel of a reaction vessel. This is a method using an apparatus.
本発明は、石英などの紫外線を利用できる素材を用いて反応容器を作製して、安価な蛍光試薬を利用して検出できる装置および方法である。
The present invention is an apparatus and method capable of producing a reaction vessel using a material such as quartz that can use ultraviolet rays and detecting it using an inexpensive fluorescent reagent.
本発明は、使用した反応容器は、高温で処理して容器内に残留した有機物を分解処理して、再利用することができ、遺伝子増幅操作にともなう廃棄物の処理負荷を軽減できる方法である。
The present invention is a method in which a used reaction vessel can be treated at a high temperature to decompose organic substances remaining in the vessel and reused, thereby reducing the waste processing load associated with gene amplification operations. .
本発明は、反応容器の部分を異なる複数の温度にすることにより、反応容器内に形成させた毛細管流路に、異なる温度領域を作ることができ、その流路を、試料が通過することにより、ＰＣＲ反応の変性、アニーリング、伸長の反応ステップを、繰り返し行えることができ、反応容器の毛細管流路内での試料の移動に伴い、遺伝子増幅反応ができる方法である。
In the present invention, different temperature regions can be created in the capillary channel formed in the reaction vessel by setting the temperature of the reaction vessel part to a plurality of different temperatures, and the sample passes through the channel. In this method, the PCR reaction denaturation, annealing, and extension reaction steps can be repeated, and a gene amplification reaction can be performed as the sample moves in the capillary channel of the reaction vessel.
本発明は、反応容器内の毛細管流路のいずれの部分でも、遺伝子の増幅に伴う蛍光強度の増加を検出できる装置および方法であり、安価な可視光の複数の受光素子を反応容器の側面に配置することにより、同時に、複数点で、遺伝子の増幅度合いを検知できる装置および方法である。
The present invention is an apparatus and method capable of detecting an increase in fluorescence intensity accompanying gene amplification in any part of a capillary channel in a reaction vessel, and a plurality of inexpensive visible light receiving elements are provided on the side of the reaction vessel. It is an apparatus and method capable of detecting the degree of gene amplification at a plurality of points simultaneously by arranging.
本発明の光源としては、紫外線灯や可視光または紫外光を発する発光ダイオードや半導体レーザーやレーザー装置を用いることができる。
As the light source of the present invention, an ultraviolet lamp, a light emitting diode that emits visible light or ultraviolet light, a semiconductor laser, or a laser device can be used.
本発明で用いる蛍光試薬は、アガロースゲル電気泳動やアクリルアミドゲル電気泳動などで用いられているエチジウムブロマイドやサイバーグリーンなどを用いることもでき、アクリジンオレンジなどの微生物染色などに用いられる蛍光試薬を用いることができる。
As the fluorescent reagent used in the present invention, ethidium bromide or cyber green used in agarose gel electrophoresis or acrylamide gel electrophoresis can be used, and a fluorescent reagent used for microbial staining such as acridine orange should be used. Can do.
本発明では、試料を反応容器の毛細管流路に導入して遺伝子増幅反応を行ってもよく、遺伝子増幅反応の組成を用いて、連続的に、あるいは、断続的に希釈して、毛細管流路に導入してもよく、断続的に希釈する場合は、希釈試料間にミネラルオイルなどを導入して、希釈試料間の区分けをしてもよい。
In the present invention, a sample may be introduced into a capillary channel of a reaction vessel to carry out a gene amplification reaction. Using the composition of the gene amplification reaction, the sample may be diluted continuously or intermittently to obtain a capillary channel. In the case of diluting intermittently, mineral oil or the like may be introduced between the diluted samples to classify the diluted samples.
本発明での試料を反応容器の毛細管流路を移動させるためには、シリンジ型ポンプを用いてもよく、ぺリスター型のポンプでもよく、ダイヤフラム式ポンプでもよく、圧電素子を用いたバイモルフ型のポンプでもよく、ＨＰＬＣのポンプでもよく、あるいは、電気浸透流による押し出しでもよく、表面弾性波による送液方法でもよい。
In order to move the sample in the present invention through the capillary channel of the reaction vessel, a syringe type pump, a peristaltic type pump, a diaphragm type pump, or a bimorph type using a piezoelectric element may be used. It may be a pump, an HPLC pump, extrusion by electroosmotic flow, or a liquid feeding method by surface acoustic waves.
本発明での、遺伝子増幅法は、ＰＣＲ法でもよく、ＬＡＭＰ法でもよい。
The gene amplification method in the present invention may be a PCR method or a LAMP method.
本発明によれば、簡易な装置構成にて遺伝子増幅操作を行い、かつ、遺伝子増幅の度合いを検知できる。
According to the present invention, gene amplification operation can be performed with a simple apparatus configuration, and the degree of gene amplification can be detected.
本発明によれば、既存の安価な蛍光色素を利用できるので、安価な遺伝子増幅操作ができる。
According to the present invention, since an existing inexpensive fluorescent dye can be used, an inexpensive gene amplification operation can be performed.
本発明によれば、光源として安価な紫外灯やＬＥＤなどを利用し、検出には安価な受光素子が利用することができ、簡易で安価な遺伝子増幅装置が構築できる。
According to the present invention, an inexpensive ultraviolet lamp or LED can be used as a light source, an inexpensive light receiving element can be used for detection, and a simple and inexpensive gene amplification device can be constructed.
本発明によれば、反応容器を高温処理することにより、流路内に付着・残留した有機物を除去でき、反応容器が再利用でき廃棄物の排出負荷を軽減できるとともに、高温処理によりウィルスや細菌などの病原微生物を死滅させることができるので、遺伝子増幅操作を衛生的に実施できる。
According to the present invention, by subjecting the reaction vessel to high temperature treatment, organic substances adhering to and remaining in the flow path can be removed, the reaction vessel can be reused, and the waste discharge load can be reduced. Since pathogenic microorganisms such as can be killed, gene amplification can be performed in a sanitary manner.
本発明の装置と方法によれば、食品・飲料、乳、乳製品、医薬品、医薬部外品、化粧品、人間または動植物から採取した組織・血液などの試料、環境試料、土壌など遺伝子増幅法を適用して分析、検査されている対象物に対して、簡易に、遺伝子増幅操作と、遺伝子増幅の度合いの検知ができる。
According to the apparatus and method of the present invention, gene amplification methods such as food / beverage, milk, dairy products, pharmaceuticals, quasi-drugs, cosmetics, tissue / blood samples collected from humans or animals and plants, environmental samples, soil, etc. It is possible to easily detect a gene amplification operation and the degree of gene amplification for an object that is applied and analyzed and inspected.
本発明の例示を以下に示すが、光源や容器の素材種類や容器の形状や流路形状は、例示に示す方法に限定されるものではない。
Illustrative examples of the present invention are shown below. However, the light source, the material type of the container, the shape of the container, and the shape of the flow path are not limited to the methods shown in the examples.
図１に示したように、本発明の実施形態である装置に用いる反応容器は、金型で作製した石英素材の反応容器１と、その反応容器内において、螺旋状である毛細管流路２と、試料導入口３で構成される。反応容器の全体形状は、図１では、立方体を支援したが、底面が円状の円柱状であっても、三角柱や六角柱など、底面が多角形の形状のものであってもよい。
As shown in FIG. 1, the reaction vessel used in the apparatus according to the embodiment of the present invention includes a quartz reaction vessel 1 made of a mold, and a capillary channel 2 that is spiral in the reaction vessel. And the sample inlet 3. In FIG. 1, the overall shape of the reaction vessel is a cube, but it may be a cylindrical column with a circular bottom surface or a polygonal bottom surface such as a triangular prism or a hexagonal column.
図２に示したように、本発明の装置の主要な構造は、反応容器１と、反応容器１の螺旋状の毛細管流路部分を加温設定できるように、反応容器１を収納できる構造を持つホルダー４と、そのホルダーに挿入設置された反応容器の１つの側面をＤＮＡの変性温度で例えば９５℃に温度設定するためのヒーター５、毛細管流路の流れ方向に沿った、反応容器の別の側面をプライマーがアニールする温度で、例えば５５℃に温度設定するためのヒーター６、反応容器のもう一つの側面を伸長反応する温度で、例えば７２℃に設定するためのヒーター７を有し、かつ、増幅した試料を紫外線励起するための光源ＬＥＤ８を配置し、試料から発せされる蛍光を測定する為の受光ダイオード９を配置できる構造である。
As shown in FIG. 2, the main structure of the apparatus of the present invention is a structure that can accommodate the reaction vessel 1 so that the reaction vessel 1 and the spiral capillary channel portion of the reaction vessel 1 can be heated. Holder 4, a heater 5 for setting the temperature of one side of the reaction vessel inserted and installed in the holder to a denaturation temperature of DNA, for example, 95 ° C., and the reaction vessel along the flow direction of the capillary channel A
試料導入の方法は、試料導入口３に、増幅反応溶液に混合した測定対象試料を載せて、ポンプから増幅反応溶液を任意の速度で送液することで、反応容器の毛細管流路内に導入させ、以降、ポンプの液押し出しにより、試料を、毛細管流路内を移動させて、増幅反応を進行させる。
The sample introduction method is such that the sample to be measured mixed with the amplification reaction solution is placed on the sample introduction port 3, and the amplification reaction solution is fed from the pump at an arbitrary speed to be introduced into the capillary channel of the reaction vessel. Thereafter, the sample is moved in the capillary channel by liquid extrusion of the pump, and the amplification reaction is allowed to proceed.
試料導入口部分に、試料貯留の空間を設けて、増幅反応溶液を試料貯留の空間に導入して、試料貯留の空間内で、試料と増幅反応溶液を希釈混合させつつ、試料貯留の空間内の液の一部を反応容器内の毛細管流路内に導入を行なうことで、導入試料の連続的な希釈を行うことができる。
A sample storage space is provided at the sample inlet, the amplification reaction solution is introduced into the sample storage space, and the sample and the amplification reaction solution are diluted and mixed in the sample storage space. By introducing a part of the solution into the capillary channel in the reaction vessel, the introduced sample can be continuously diluted.
試料の希釈導入により、試料中の増幅対象ＤＮＡの濃度が変化するので、反応容器の螺旋状毛細管流路内の増幅試料由来の増幅信号の発生位置・強度が変化する。その変化情報と、希釈倍率・希釈速度、毛細管流路内の反応溶液の送液量などの情報を組み合わせることにより、試料中の増幅対象のＤＮＡの濃度が正確に測定できる。
Since the concentration of DNA to be amplified in the sample is changed by introducing the sample into the dilution, the position and intensity of the amplification signal derived from the amplified sample in the spiral capillary channel of the reaction vessel are changed. The concentration of DNA to be amplified in the sample can be accurately measured by combining the change information with information such as the dilution rate / dilution speed and the amount of the reaction solution in the capillary channel.
反応試料と遺伝子増幅反応溶液との混合比率は、反応試料が反応容器内の毛細管流露に流入するに従い、希釈倍率が高くなり、反応容器の毛細管流路の螺旋の回転数が増加しても、増幅された遺伝子が少なくなるため、蛍光色素の信号が低下し、希釈比率が大きくない反応操作初期に反応容器の毛細管流路に導入された試料での蛍光強度と比較しながら、定量的に、試料中に存在する対象とする遺伝子の数が推定される。
The mixing ratio of the reaction sample and the gene amplification reaction solution is such that, as the reaction sample flows into the capillary flow in the reaction vessel, the dilution rate increases, and even if the spiral rotation speed of the capillary channel of the reaction vessel increases, Since the number of amplified genes decreases, the fluorescent dye signal decreases and the dilution ratio is not large. While comparing with the fluorescence intensity in the sample introduced into the capillary channel of the reaction vessel at the initial stage of the reaction operation, quantitatively, The number of genes of interest present in the sample is estimated.
遺伝子増幅を行う対象試料は、反応容器とポンプ部分の間に導入され、ポンプの送液の作用により、反応容器内の毛細管流露に導入される。
The target sample to be subjected to gene amplification is introduced between the reaction container and the pump part, and is introduced into the capillary flow in the reaction container by the action of the pump solution.
反応試料を導入する空間に反応試料を導入して、その反応試料を導入する空間に対して、遺伝子増幅反応溶液の成分である耐熱酵素、その反応基質のｄＡＴＰ、ｄＧＴＰ、ｄＣＴＰおよびｄＴＴＰや、プライマーとなるオリゴＤＮＡや、エチジウムブロマイドのような蛍光色素を含む溶液を導入し、反応試料と連続して混合している。
The reaction sample is introduced into the space into which the reaction sample is introduced, the thermostable enzyme that is a component of the gene amplification reaction solution, dATP, dGTP, dCTP and dTTP of the reaction substrate, primers, A solution containing an oligo DNA and a fluorescent dye such as ethidium bromide is introduced and continuously mixed with the reaction sample.
本発明の方法により、簡易な装置構成により、遺伝子増幅操作と遺伝子増幅の増幅度合いの検知する装置が構築できる。
By the method of the present invention, it is possible to construct a device for detecting gene amplification operation and the amplification degree of gene amplification with a simple device configuration.
本発明の方法により、特別の操作手法を要せず、簡易に、遺伝子増幅操作と、増幅度合いの検知をができる装置を構築できる。
By the method of the present invention, it is possible to construct a device that can easily perform a gene amplification operation and detect the degree of amplification without requiring a special operation technique.
本発明の方法により、安価な蛍光試薬が適用でき、遺伝子増幅操作と増幅確認操作を安価に実施できる。
By the method of the present invention, an inexpensive fluorescent reagent can be applied, and the gene amplification operation and the amplification confirmation operation can be performed at low cost.
本発明の方法により、反応容器を高温で処理して再利用も可能であるので、遺伝子増幅操作と、遺伝子増幅の確認、分析操作に伴う使い捨て廃棄物の発生を抑制できる。
According to the method of the present invention, the reaction vessel can be treated and reused at a high temperature, so that the generation of disposable waste accompanying the gene amplification operation, the confirmation of gene amplification, and the analysis operation can be suppressed.
本発明により、食品・飲料、乳、乳製品、医薬品、医薬部外品、化粧品、人間または動植物から採取した組織・血液などの試料、環境試料、土壌など遺伝子増幅法を適用して分析、検査されている対象物に対して、簡易に、安価に、かつ、安全・衛生的に、遺伝子増幅操作と、遺伝子増幅の度合いの検知ができる。
According to the present invention, analysis and inspection are performed by applying gene amplification methods such as food / beverage, milk, dairy products, pharmaceuticals, quasi-drugs, cosmetics, tissue / blood samples collected from humans or animals and plants, environmental samples, soil, etc. It is possible to simply and inexpensively and safely and hygienically detect a gene amplification operation and the degree of gene amplification with respect to a target object.
１ 反応容器
２ 毛細管流路
３ 試料導入口
４ ホルダー
５ ヒーター
６ ヒーター
７ ヒーター
８ 光源ＬＥＤ
９ 受光ダイオード
1 Reaction vessel 2 Capillary flow path 3 Sample inlet 4 Holder 5
9 Light receiving diode
Claims (5)
This is a simple method for quantifying DNA or RNA using a gene amplification method. While performing the amplification reaction while moving in the capillary channel of the reaction vessel holding the capillary channel, the amplification degree is simultaneously measured. Apparatus and method for monitoring
The sample to be introduced into the capillary channel of the reaction vessel is introduced into the capillary channel of the reaction vessel while being continuously or intermittently diluted and mixed with the solution containing the starting reaction component of the amplification reaction. Apparatus and method according to claim 1.
3. The apparatus and method according to claim 1, wherein the reaction vessel has a rod-like shape, a cylindrical shape, or a cubic shape, and the capillary channel of the reaction vessel has a spiral shape.
The amplification degree of a gene amplification reaction is monitored using a reagent that emits a fluorescent signal by binding to DNA or RNA, such as ethidium bromide, acridion orange, or cyber green. And the apparatus and method of claim 3
A reaction vessel made of quartz is used, which is manufactured by forming a capillary channel using a mold made of quartz powder as a raw material and vitrifying it. Item 4 Apparatus and Method
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|---|---|---|---|
| JP2008028667A JP2009183244A (en) | 2008-02-08 | 2008-02-08 | Simple device for quantitative determination by gene amplification |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008028667A JP2009183244A (en) | 2008-02-08 | 2008-02-08 | Simple device for quantitative determination by gene amplification |
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| JP2009183244A true JP2009183244A (en) | 2009-08-20 |
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| JP2008028667A Pending JP2009183244A (en) | 2008-02-08 | 2008-02-08 | Simple device for quantitative determination by gene amplification |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013226497A (en) * | 2012-04-25 | 2013-11-07 | Panasonic Corp | Micro fluid device |
| CN109022263A (en) * | 2017-06-08 | 2018-12-18 | 昆泰锐（武汉）生物技术有限责任公司 | A kind of tubular type temperature regulating device and the reaction instrument comprising the tubular type temperature regulating device |
| CN110452803A (en) * | 2019-08-27 | 2019-11-15 | 东南大学 | A kind of nucleic acid rapid amplifying detection method and device |
| JP2020110092A (en) * | 2019-01-13 | 2020-07-27 | テクノグローバル株式会社 | Gene detection system, gene detection chip, gene detection apparatus, gene amplification method, and gene amplification apparatus |
-
2008
- 2008-02-08 JP JP2008028667A patent/JP2009183244A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013226497A (en) * | 2012-04-25 | 2013-11-07 | Panasonic Corp | Micro fluid device |
| CN109022263A (en) * | 2017-06-08 | 2018-12-18 | 昆泰锐（武汉）生物技术有限责任公司 | A kind of tubular type temperature regulating device and the reaction instrument comprising the tubular type temperature regulating device |
| JP2020110092A (en) * | 2019-01-13 | 2020-07-27 | テクノグローバル株式会社 | Gene detection system, gene detection chip, gene detection apparatus, gene amplification method, and gene amplification apparatus |
| CN110452803A (en) * | 2019-08-27 | 2019-11-15 | 东南大学 | A kind of nucleic acid rapid amplifying detection method and device |
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