JP6313027B2 - Chemical liquid supply device and flowing water type fish test device - Google Patents

Description

  The present invention relates to a chemical supply apparatus and a flowing water type used in a flowing water fish test for evaluating biological or chemical safety of toxic organic substances, chemical substances, etc. contained in industrial waste, factory waste water, agricultural chemicals, etc. The present invention relates to a fish testing apparatus.

  Conventionally, specimens have been bred in test water formed from chemicals and dilution water containing substances (test substances) containing toxic organic substances and chemical substances (test substances) contained in industrial waste, industrial wastewater, agricultural chemicals, etc. A flowing-water fish test is known in which the biological or chemical safety of a test substance is evaluated by observing the living body of a specimen (Patent Document 1). As shown in FIG. 5, a conventional flowing-water fish test apparatus 100 used for such a flowing-water fish test stores a chemical liquid tank 102 for storing a chemical liquid containing a test substance, and diluted water for diluting the chemical liquid. A diluting water tank 104, a water tank 106 for mixing these chemicals and diluting water to a predetermined concentration and storing them as test water, a test water tank 110 for raising the test fish 108 in the test water, and a chemical liquid tank A chemical solution supply pump 112 that supplies the chemical solution in 102 to the water storage tank 106 and a dilution water supply pump 114 that supplies the dilution water in the dilution water tank 104 to the water storage tank 106 are provided.

JP-A-52-133287

  In general, many substances such as toxic organic substances and chemical substances contained in industrial waste, factory effluent, agricultural chemicals, etc. are hardly water-soluble (hydrophobic), and are therefore used in the conventional flowing-water fish testing apparatus 100. The chemical solution is prepared by mixing a test substance with a solvent such as an organic solvent capable of dissolving these substances. For this reason, in the flowing-water fish test using the conventional flowing-water fish testing apparatus 100, in addition to the test using the flowing-water fish testing apparatus 100, the influence of the solvent used to dissolve the test substance on the test fish is affected. There is a problem that it is necessary to further perform a solvent control test to be evaluated, a large test facility is required, the test becomes complicated, and analysis of ecological effects is complicated and difficult.

  Then, an object of this invention is to provide the chemical | medical solution supply apparatus and flowing water type fish test apparatus which can perform the flowing water type fish test which does not require a solvent control test.

  In order to achieve the above object, a chemical solution supply device according to the present invention is a chemical solution supply device for supplying a chemical solution comprising water and a test substance, and is provided in the chemical solution water tank and the chemical solution water tank. A holding member capable of holding the test substance and allowing water to pass through, a flow means capable of flowing the water in the chemical solution tank, and a chemical solution prepared in the chemical solution tank. And a supply means that can be supplied to the outside of the water tank.

  In the chemical solution supply apparatus according to the present invention, the holding member is configured to be able to hold the test substance by dipping in a solution in which the test substance is dissolved and then drying to evaporate the solvent. Is preferred.

  Further, the flowing water type fish test apparatus according to the present invention comprises a chemical solution supply means for supplying a chemical solution to a test water tank in which a test specimen is bred with test water mixed with the chemical solution and dilution water, and dilution water is supplied to the test water tank. A flowing water type fish test apparatus comprising a dilution water supply means for supplying the chemical liquid supply means, the chemical liquid supply means being provided in the chemical liquid water tank and the chemical water tank, capable of holding a test substance, and allowing water to pass therethrough. A possible holding member, a flow means capable of flowing the water in the chemical liquid tank, and a supply means capable of supplying the chemical liquid created in the chemical liquid tank to the outside of the chemical liquid tank. It is characterized by providing.

  As described above, according to the present invention, it is possible to provide a chemical solution supply apparatus and a flowing water fish testing apparatus capable of performing a flowing water fish test that does not require a solvent control test.

It is a conceptual diagram which shows schematic structure of the flowing-water type fish test apparatus which concerns on one Embodiment of this invention. It is a conceptual diagram which shows schematic structure of the water tank for chemical | medical solutions of the flowing-water type fish test apparatus which concerns on this embodiment. It is a control block diagram of the flowing-water type fish test apparatus concerning this embodiment. It is a conceptual diagram which shows schematic structure of the flowing-water type fish test apparatus which concerns on other embodiment of this invention. It is a conceptual diagram which shows schematic structure of the conventional flowing-water type fish test apparatus.

  Next, a flowing-water fish testing apparatus according to an embodiment of the present invention will be described with reference to the drawings. As shown in FIG. 1, the flowing water type fish test apparatus 1 according to the present embodiment keeps a specimen 2 inhabiting in water such as fish and shellfish with test water in which a chemical solution and dilution water are mixed. The test water tank 4, the chemical liquid supply device 6 that creates a chemical solution from the water and the test substance and supplies the chemical water to the test water tank 4, the dilution water supply device 8 that supplies the dilution water to the test water tank 4, the chemical liquid supply device 6 and the dilution And a control unit A (see FIG. 3) for controlling the water supply device 8.

  The test water tank 4 is equipped with various devices such as an oxygen supply device (not shown) so that the specimen 2 can swim, and the test water is excessive on the side surface by the chemical solution supply device 6 and the dilution water supply device 8. An overflow pipe 5 is provided for discharging the excess test water when supplied to the tank.

  The chemical liquid supply device 6 creates a chemical liquid to be supplied to the test water tank 4 and stores it, a water supply means 12 connected to the chemical liquid water tank 10 and supplying water to the chemical water tank 10, A holding member 14 provided in the chemical liquid tank 10 and configured to hold a test substance, a flow means 16 for flowing water in the chemical liquid tank 10, and a chemical prepared in the chemical liquid tank 10 are tested. A supply means 18 for supplying to the water tank 4 and a liquid level sensor 20 configured to detect the position of the liquid level of the chemical liquid stored in the chemical water tank 10 are provided.

  As shown in FIG. 2, the chemical water tank 10 is erected so that a box-shaped water tank body 22 made of glass or the like and a chemical liquid circulation channel (outward path 26 a and return path 26 b) are formed in the water tank body 22. It is comprised from the partition plate 24 made. FIG. 2 is a conceptual diagram schematically showing the internal structure of the chemical liquid tank 10 when the chemical liquid tank 10 is viewed from above. The partition plate 24 includes a first partition plate 24a extending in parallel to the front and back surfaces of the water tank body 22 so as to bisect the water tank body 22 in the front-rear direction (vertical direction in FIG. 2), and one end of the first partition plate 24a. From the first partition plate 24a to the rear surface of the water tank body 22, and the second partition plate 24b to the fifth partition plate 24e, which are provided in parallel at equal intervals from the first to the other end. The aquarium body so as to be positioned in parallel between the second partition plate 24c and the third partition plate 24c, between the third partition plate 24c and the fourth partition plate 24d, and between the fourth partition plate 24d and the fifth partition plate 24e. 22, the sixth partition plate 24 f to the eighth partition plate 24 h extending from the back surface of the 22 toward the first partition plate 24 a. The first partition plate 24a has such a length that a predetermined interval is formed between both ends thereof and both side surfaces of the water tank body 22, and the second partition plate 24b to the fifth partition plate 24e are The sixth partition plate 24f to the eighth partition plate 24h have a length such that a predetermined interval is formed between the tip and the back surface of the water tank body 22. The sixth partition plate 24f to the eighth partition plate 24h It has such a length that a predetermined interval is formed between it and 24a. In the chemical water tank 10, the partition plate 24 is arranged in this manner, whereby a chemical liquid circulation channel including a straight forward path 26 a and a meandering return path 26 b is formed. A discharge port (not shown) for discharging the chemical solution is formed on the bottom surface of the chemical solution tank 10, and an upstream chemical solution communication pipe 48 (to be described later) of the supply means 18 is connected to the discharge port. Yes.

  As shown in FIG. 1, the water supply means 12 includes a water tank 30 that stores water to be supplied to the chemical liquid tank 10 and a water supply pump 32 that supplies water in the water tank 30 to the chemical liquid tank 10. I have. The water supply pump 32 is configured such that its drive is controlled by the control unit A, as will be described later. The water supplied from the water tank 30 to the chemical solution tank 10 may be pure water or unsaturated water containing a test substance, but unsaturated water (for example, a dissolved solution of about 70%). Is preferable because the time until saturation (for example, 90% or more) in the chemical water tank 10 can be shortened as compared with the case where pure water is used.

  The holding member 14 is formed of a member capable of holding the test substance and allowing water to pass therethrough, for example, a member having a large surface area such as cotton, nonwoven fabric, or fiber. For example, the holding member 14 is soaked in a solution in which a test substance is dissolved and then dried to evaporate the solvent, thereby attaching and holding the test substance on the surface and inside thereof. As shown in FIGS. 1 and 2, the holding member 14 is filled or disposed in a predetermined amount in the circulation flow path (the forward path 26a and the return path 26b) of the chemical water tank 10 in a state where the test substance is held on the surface and inside thereof. ing.

  The flow means 16 includes a stirring member 34 composed of a rotating shaft and a stirring blade attached to the tip of the rotating shaft, and a motor 36 for rotating the stirring member 34. The stirring blade of the stirring member 34 is the chemical water tank 10. It is attached to the chemical water tank 10 so as to be located inside. The flow means 16 is configured to stir the chemical solution in the chemical solution water tank 10 by the stirring member 34 and to flow the chemical solution along the circulation flow path (the forward path 26a and the return path 26b).

  As shown in FIG. 1, the supply means 18 includes a chemical liquid measurement container 40 that measures the chemical liquid supplied to the test water tank 4, an upstream chemical liquid supply unit 42 that supplies the chemical liquid to the chemical liquid measurement container 40, and the measured chemical liquid. A downstream chemical liquid supply unit 44 that supplies the test water tank 4 and a chemical sensor 46 that detects whether or not a predetermined amount of chemical liquid has accumulated in the chemical liquid measurement container 40 are provided. The chemical liquid measurement container 40 is provided below the chemical liquid water tank 10 and above the test water tank 4, and an upstream chemical liquid supply unit 42 is provided between the chemical liquid water tank 10 and the chemical liquid measurement container 40, A downstream chemical liquid supply unit 44 is provided between the chemical liquid measurement container 40 and the test water tank 4.

  The upstream chemical liquid supply unit 42 is connected to the bottom surface of the chemical liquid tank 10 and is provided in the upstream chemical liquid communication pipe 48 that communicates the chemical liquid water tank 10 and the chemical liquid measuring container 40, and the upstream chemical liquid communication pipe 48. And an upstream side chemical solution electromagnetic valve 50 capable of opening and closing the upstream side chemical solution communication pipe 48.

  The downstream chemical liquid supply unit 44 is connected to the bottom surface of the chemical liquid measurement container 40 and is provided in the downstream chemical liquid communication pipe 52 that communicates the chemical liquid measurement container 40 and the test water tank 4 and the downstream chemical liquid communication pipe 52. And a downstream chemical liquid electromagnetic valve 54 that can open and close the side chemical liquid communication pipe 52.

  The downstream side of the downstream chemical liquid communication pipe 52 is joined to the downstream side of the downstream dilution water communication pipe 72 (described later) of the dilution water supply device 8. When the electromagnetic valve 74 for water is opened, it is configured to be supplied to the test water tank 4 in a state where the dilution water and the chemical solution are mixed, that is, in the state of test water.

  As shown in FIG. 1, the dilution water supply device 8 includes a dilution water tank 60 for storing dilution water to be supplied to the test water tank 4, a dilution water measuring container 62 for measuring dilution water to be supplied to the test water tank 4, and An upstream dilution water supply unit 64 that supplies dilution water to the dilution water measuring container 62, a downstream dilution water supply unit 66 that supplies the measured dilution water to the test water tank 4, and a predetermined amount in the dilution water measuring container 62. A dilution water sensor 68 is provided for detecting whether or not the dilution water has accumulated. The dilution water measuring container 62 is provided above the test water tank 4, and the dilution water tank 60 is provided above the dilution water measuring container 62. An upstream side dilution water supply unit 64 is provided between the dilution water tank 60 and the dilution water metering container 62, and a downstream side dilution water supply unit 66 is provided between the dilution water metering container 62 and the test water tank 4. It has been.

  The upstream dilution water supply unit 64 is connected to the bottom surface of the dilution water tank 60, and communicates the dilution water tank 60 and the dilution water metering container 62 with the upstream dilution water communication pipe 69, and the upstream dilution water communication pipe. 69 and an upstream dilution water electromagnetic valve 70 capable of opening and closing the upstream dilution water communication pipe 69.

  The downstream dilution water supply unit 66 is connected to the bottom surface of the dilution water metering container 62, and communicates the dilution water metering container 62 and the test water tank 4 with the downstream dilution water communication pipe 72 and the downstream dilution water communication pipe. 72 and a downstream dilution water electromagnetic valve 74 that can open and close the downstream dilution water communication pipe 72.

  As shown in FIG. 3, the control unit A includes a liquid level sensor 20 that detects the liquid level position of the chemical liquid tank 10, a water supply pump 32 that supplies water to the chemical liquid tank 10, and an upstream side of the chemical liquid supply apparatus 6. The chemical solution solenoid valve 50, the downstream chemical solution solenoid valve 54 and the chemical solution sensor 46, the upstream dilution water electromagnetic valve 70, the downstream dilution water electromagnetic valve 74 and the dilution water sensor 68 of the dilution water supply device 8, respectively. It is connected.

  The control unit A mainly includes a water supply control for supplying water to the chemical water tank 10, a chemical liquid measurement control for measuring the chemical liquid supplied to the test water tank 4, and a dilution for measuring the dilution water supplied to the test water tank 4. The water metering control and the test water supply control for supplying the test water tank 4 in a state where the chemical solution and the dilution water are mixed are executed.

  The water supply control by the control unit A is performed when the control unit A detects that the liquid level of the chemical solution in the chemical solution tank 10 has reached a predetermined upper limit position by the liquid level sensor 20. When the supply of water is stopped and the liquid level sensor 20 detects that the liquid level of the chemical liquid in the chemical liquid tank 10 has reached the predetermined lower limit position, the supply of water to the chemical liquid tank 10 is started. In this way, the operation of the water supply pump 32 is controlled.

  The chemical metering control by the control unit A is performed on the upstream side until the controller A detects that a predetermined amount of chemical solution has been stored in the chemical metering container 40 by the chemical sensor 46 after the downstream chemical solution electromagnetic valve 54 is closed. The operation is performed by opening the chemical liquid solenoid valve 50 and closing the upstream chemical liquid solenoid valve 50 when it is detected that a predetermined amount of chemical liquid is stored in the chemical liquid measurement container 40.

  In the dilution water metering control by the control unit A, after the control unit A closes the downstream dilution water electromagnetic valve 74, the dilution water sensor 68 detects that a predetermined amount of dilution water has accumulated in the dilution water metering container 62. The upstream dilution water electromagnetic valve 70 is opened until the upstream dilution water electromagnetic valve 70 is closed when it is detected that a predetermined amount of dilution water has accumulated in the dilution water measuring container 62. .

  The test water supply control by the control unit A is performed by the control unit A while the upstream chemical solution electromagnetic valve 50 and the upstream dilution water electromagnetic valve 70 are open. When the electromagnetic valve for water 74 is closed and all of the electromagnetic valve for upstream chemical solution 50 and the electromagnetic valve for upstream dilution water 70 are closed, or after a predetermined time has elapsed, the electromagnetic valve for downstream chemical solution 54 and the downstream dilution valve are diluted. This is done by opening the water solenoid valve 74. As a result, the chemical liquid measured by the chemical liquid measurement control and the dilution water measured by the dilution water measurement control are mixed at the junction of the downstream chemical liquid communication pipe 52 and the downstream dilution water communication pipe 72 to obtain test water. And supplied to the test water tank 4.

  Next, operation | movement of the flowing-water type fish test apparatus which concerns on this embodiment is demonstrated. First, prior to the supply operation of the chemical solution and the diluting solution, the chemical solution preparation operation in the chemical solution water tank 10 is performed. Specifically, first, a holding member 14 to which a test substance is attached is filled or arranged in a predetermined amount in the circulation flow path (outward path 26a and return path 26b) of the chemical solution water tank 10, and water (pure water) is supplied from the water tank 30. Alternatively, a predetermined amount of unsaturated water) is supplied into the chemical water tank 10. The amount of water supply is appropriately adjusted by the control unit A controlling the water supply pump 32 based on the detection result of the liquid level sensor 20. Thereafter, the water in the chemical water tank 10 is circulated by operating the flow means 16. In this way, by causing the water in the chemical solution tank 10 to flow and pass through the holding member 14, the test substance held by the holding member 14 is gradually dissolved in water, and the test substance is saturated with water. By such an operation, a chemical solution in which the test substance is saturated can be prepared.

  In the state where the chemical solution is prepared and stored in the chemical solution water tank 10 in this way, the supply operation of the chemical solution and the diluted solution is executed. Specifically, first, the downstream dilution water electromagnetic valve 74 and the downstream chemical liquid electromagnetic valve 54 are closed, and the upstream dilution water electromagnetic valve 70 and the upstream chemical liquid electromagnetic valve 50 are opened. Then, the dilution water is supplied from the dilution water tank 60 to the dilution water measuring container 62, and the chemical solution is supplied from the chemical solution water tank 10 to the chemical solution measuring container 40, and the dilution water and the chemical solution are respectively stored. Thereafter, when the dilution water sensor 68 detects that a predetermined amount of dilution water has accumulated in the dilution water metering container 62, the upstream dilution water electromagnetic valve 70 is closed. In parallel with this, when the chemical liquid sensor 46 detects that a predetermined amount of chemical liquid has accumulated in the chemical liquid measurement container 40, the upstream chemical liquid electromagnetic valve 50 is closed. Of the upstream dilution solenoid valve 70 and the upstream chemical solution solenoid valve 50, the downstream dilution solenoid valve 74 and the downstream chemical solution solenoid valve 54 after a predetermined time elapses from the closing time of the later closing time or the closing time. Is opened, and the dilution water in the dilution water measuring container 62 and the chemical liquid in the chemical liquid measuring container 40 are mixed and supplied to the test water tank 4. When all of the dilution water in the dilution water measuring container 62 and the chemical liquid in the chemical liquid measurement container 40 are supplied to the test water tank 4, the downstream dilution water electromagnetic valve 74 and the downstream chemical liquid electromagnetic valve 54 are closed. Then, by repeatedly supplying the dilution water and the chemical solution in this manner, the test water is continuously supplied to the test water tank 4 and the safety of the test substance 2 in the test water tank 4 is evaluated for safety.

  According to the flowing water type fish test apparatus 1 according to the present embodiment, a chemical water tank 10, a holding member 14 provided in the chemical liquid water tank 10, capable of holding a test substance and allowing water to pass through, and a chemical liquid. A chemical solution supply device 6 comprising a flow means 16 capable of causing the water in the water tank 10 to flow and a supply means 18 capable of supplying the chemical liquid prepared in the chemical water tank 10 to the test water tank 4. By providing, it is possible to conduct a flowing water fish test that does not require a solvent control test. That is, in the chemical solution supply device 6 of the flowing water type fish test apparatus 1 according to the present embodiment, the chemical solution is prepared by flowing water (pure water or unsaturated water) supplied from the water supply means 12 in the chemical solution tank 10. The test substance adhering to the holding member 14 is gradually dissolved in water. Thus, in the flowing-water fish test apparatus 1 according to the present embodiment, the organic solvent used in the conventional flowing-water fish test is not present in the chemical water tank 10 and is not contained in the test water. It is not necessary to perform a solvent control test for investigating the influence of the solvent on the specimen 2, thereby eliminating the need for a test facility for performing the solvent control test and simplifying the test. Ecological impact analysis can be made simple and easy.

  In addition, the flowing water type fish test apparatus 1 according to the present embodiment is installed in the order of the chemical liquid tank 10 and the dilution water tank 60 → the chemical liquid measurement container 40 and the dilution water measurement container 62 → the test water tank 4 from above. In addition, since the chemical solution, the dilution water, and the test water can be caused to flow by their own weight, a flowing water type fish test device that does not use a driving device such as a pump can be obtained.

  The flowing-water fish testing apparatus according to the present invention is not limited to the above-described embodiment, and various modifications can be made without departing from the technical idea of the present invention. For example, although the flowing-water type fish test apparatus 1 according to the present embodiment has been described as supplying test water to one test water tank 4, the present invention is not limited thereto. For example, as shown in FIG. 4 may be supplied with test water. In this case, the supply means 18 for supplying the chemical solution prepared in the chemical water tank 10 to the test water tank 4 and the means for supplying the diluted water in the dilution water tank 60 to the test water tank 4 (dilution water measuring container 62, upstream side) A dilution water supply unit 64, a downstream dilution water supply unit 66, and a dilution water sensor 68) may be provided for each test water tank 4.

  Moreover, although the flowing-water type fish test apparatus 1 which concerns on this embodiment demonstrated as what supplies a chemical | medical solution and dilution water directly to the test water tank 4 from the chemical | medical solution supply apparatus 6 and the dilution water supply apparatus 8, it is limited to this. First, a water storage tank is provided between the chemical liquid supply device 6 and the dilution water supply device 8 and the test water tank 4, and the chemical liquid and the dilution water from the chemical liquid supply device 6 and the dilution water supply device 8 are supplied to the water storage tank for the test. It is good also as a structure by which test water is continuously supplied to the test water tank 4 from this water tank after creating water.

  Furthermore, in the flowing water type fish test apparatus 1 according to the present embodiment, the medicinal solution water tank 10 is formed with a circulation flow path including a straight forward path 26a and a meandering return path 26b. It is not limited, and any material can be used as long as it can flow water (pure water or unsaturated water) in a state where the holding member 14 is accommodated.

  Furthermore, in the flowing water type fish test apparatus 1 according to the present embodiment, the holding member 14 is formed of a member having a large surface area such as cotton, non-woven fabric, fiber, and the like. The test material is immersed in a solution in which the test substance is dissolved, and then dried and evaporated to evaporate the solvent. However, the present invention is not limited to this, and the holding member 14 can hold the test substance. Any method may be used as long as water can pass through it, and the test substance may be attached by any method as long as the test substance can be held by the holding member 14. Further, the shape and the amount of the holding member 14 accommodated in the chemical water tank 10 can be appropriately adjusted according to the test time, the saturation state of the test substance, and the like.

DESCRIPTION OF SYMBOLS 1 Flowing-water type fish test apparatus, 2 Specimen, 4 Test water tank, 6 Chemical liquid supply means (chemical liquid supply apparatus), 8 Dilution water supply means (dilution water supply apparatus), 10 Chemical liquid water tank, 14 Holding member, 16 Flow means, 18 Supply means

Claims (3)

A chemical solution supply device for supplying a chemical solution comprising water and a test substance,
A chemical tank,
A holding member provided in the chemical water tank, capable of holding a test substance, and capable of passing water;
Fluid means capable of causing the water in the chemical water tank to flow;
A supply means capable of supplying the chemical liquid created in the chemical liquid tank to the outside of the chemical liquid tank ;
The chemical solution supply apparatus, wherein a circulation channel for circulating the chemical solution is formed in the chemical solution water tank . The said holding member is comprised so that a test substance can be hold | maintained by making it dry and evaporating a solvent after immersing in the solution in which the test substance was melt | dissolved. Chemical supply device. A flowing water type fish test comprising a chemical solution supplying means for supplying a chemical solution to a test water tank in which a test specimen is bred with test water mixed with the chemical liquid and dilution water, and a diluted water supply means for supplying dilution water to the test water tank. A device,
The chemical solution supply means includes
A chemical tank,
A holding member provided in the chemical water tank, capable of holding a test substance, and capable of passing water;
Fluid means capable of causing the water in the chemical water tank to flow;
A supply means capable of supplying the chemical liquid created in the chemical liquid tank to the outside of the chemical liquid tank ;
A circulating water fish testing apparatus, wherein a circulation channel for circulating a chemical solution is formed in the chemical solution water tank .

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