JP6298999B2 - Color measuring device and color measuring program - Google Patents

Description

  The present invention relates to a color measurement device and a color measurement program for automatically measuring the state of a sample administered to a color reagent.

A device described in Patent Document 1 is known as an apparatus for measuring a reaction of a color reagent (hereinafter referred to as a color reaction) by administering a sample to the color reagent.
In the analyzer described in Patent Document 1, when imaging the reaction region and the code region of the test piece at the same time, when the reaction region and the code region of the test piece having a difference in brightness are imaged under the same conditions, The problem is that the bit pattern appearing in the code area cannot be read properly.
The analysis apparatus described in Patent Document 1 captures an image of the analysis region including the reaction region and the code region as the image data for code reading under the first imaging condition to detect the test substance. When acquiring as image data for use, it is possible to avoid unread or misread information displayed in the code area by shooting under a second shooting condition that is different from the first shooting condition. It describes that the coloration state can be analyzed with high reliability.

JP 2012-225864 A

  However, in the analysis apparatus described in Patent Literature 1, two image files are generated, which are an image obtained by photographing optical reading information under the first photographing condition and a color reaction image photographed under the second photographing condition, Since it will be handled, management for saving and reconfirming the photographing contents later is complicated. Therefore, management can be facilitated if the optical reading information and the color reaction can be simultaneously copied to form one image file by one shooting.

  Accordingly, an object of the present invention is to provide a color measurement device and a color measurement program capable of reliably reading and accurately measuring optical reading information and color reaction even if the optical reading information and the color reaction are simultaneously photographed.

  The present invention provides a color measurement device for analyzing a color reaction (hereinafter referred to as a color reaction) in which a sample is administered and reacts with a color reagent carried on a substrate, and the color of the color reagent is analyzed. This is optical reading information indicating measurement information relating to a reaction, and a captured image of a color image by simultaneously photographing a range including the optical reading information given to the base material and the color reaction developed by the color reagent. An area sensor camera that outputs data, an illumination unit that illuminates a range including optical reading information and color reaction of the color reagent with white light mixed with three primary colors, and imaging from the area sensor camera Based on the image data, read the RGB data of the area where the optical reading information was photographed, and read the RGB data of the area where the color reaction was photographed, and extract the color reaction to this RGB data Based on the measurement processing information indicated by the optical processing information read by the image processing section and the optical reading information read by the image processing section, a measurement value corresponding to the color reaction indicated by the RGB data subjected to the filter calculation is calculated. And a measurement value calculation unit that outputs the measured value.

  According to the color measurement device of the present invention, only one area sensor camera needs to be provided in order to simultaneously capture the optical reading information and the color reaction generated by the color reagent by the area sensor camera. The image processing unit reads the RGB data of the area where the optical reading information is photographed, reads the RGB data of the area where the color reaction is photographed, and performs a filter operation for extracting the color reaction from the RGB data. I do. Then, the measurement value calculation unit calculates and outputs a measurement value corresponding to the color reaction indicated by the RGB data subjected to the filter calculation based on the measurement information indicated by the optical reading information read by the image processing unit. . Accordingly, the optical reading information is read based on RGB data based on white light, and the color reaction is performed on the RGB data by performing a filter operation for extracting the color reaction. Reading information and color reaction can be read accurately.

  In the filter calculation by the image processing unit, it is desirable to multiply the RGB data by a coefficient weighted for each of RGB in accordance with the wavelength range absorbed in the color development in the color reaction. Since the filter operation simply multiplies the RGB data by the weighting coefficient, the color reaction can be read accurately with a simple process.

  If an illuminating unit having a circular light source arranged around the photographing lens of the area sensor camera is provided, it is possible to eliminate the influence of shadows due to unevenness of the base material.

Time to measure the initial time at which the color reaction of the color reagent starts and output a shooting instruction to the image processing unit after elapse of time and output a shooting instruction to the image processing unit at every interval time after shooting It is desirable that a measurement unit is provided.
Since the time measurement unit measures the initial time for starting the color reaction and the interval time for confirming the progress of the color reaction, it is not necessary to manually measure the initial time and the interval time, and it is automatically presented. Color response can be measured.

  The image processing unit includes RGB data indicating a color of a reference pattern that is a color reference of the base material photographed in the captured image data, and RGB information indicating the RGB data of the reference pattern obtained from optical reading information Therefore, it is desirable to determine the shooting environment by the area sensor camera. Since the image processing unit determines the shooting environment from the RGB data indicating the color of the reference pattern and the RGB information of the optical reading information, it is possible to prevent the measurement value corresponding to the color reaction from being erroneously calculated. .

  Captured image data captured by the area sensor camera, including captured image data including handwritten information written on the surface of the substrate, is stored in the storage unit, and is read from the storage unit and displayed on the display unit. It is desirable to provide display control means for displaying. Even if handwritten information is entered on the base material, it is stored in the storage unit as captured image data and has a display control means for displaying on the display unit, so that the captured image data can be read at any time to check the handwritten information Can do.

  The color measurement device according to the present invention is a color measurement program for operating a computer as a color measurement device for analyzing a color reaction of an administered sample with a color reagent carried on a substrate. A range in which the computer is optical reading information indicating measurement information related to a color reaction of the color reagent, and includes optical read information applied to the base material and a color reaction generated by the color reagent. RGB of the area where the optical read information is captured based on the captured image data of the color image from the area sensor camera that is illuminated with white light mixed with primary color light and simultaneously captured and output as captured image data An image processing unit that reads data, reads RGB data of a region where the color reaction is photographed, and performs a filter operation to extract the color reaction to the RGB data Based on measurement information indicated by optical reading information read by the image processing unit, functions as a measurement value calculation unit that calculates and outputs a measurement value corresponding to the color reaction indicated by the RGB data subjected to the filter calculation This can be realized by a color measurement program characterized by

  In the present invention, it is not necessary to provide two scanners that read the optical reading information and an analysis unit that analyzes the color reaction generated by the coloring reagent, and the optical reading information is based on RGB data based on white light. Since the filter operation for reading and extracting the color reaction is performed on the RGB data, even if the optical reading information and the color reaction are photographed at the same time, it can be surely read and accurately measured. .

It is a figure which shows the whole structure of the color measurement apparatus which concerns on this Embodiment. It is a top view which shows the card type reagent which the color measurement apparatus shown in FIG. 1 analyzes. It is a format figure of the measurement information recorded on the two-dimensional barcode of the card type reagent shown in FIG. It is a block diagram for demonstrating the structure of the color measurement apparatus shown in FIG. It is a figure for demonstrating the area sensor camera incorporated in the color measurement apparatus shown in FIG. It is a figure of an example of the setting screen displayed by the display control part on the display part of the computer of the color measurement apparatus shown in FIG. It is a figure of an example of the operation screen displayed by the display control part on the display part of the computer of the color measurement apparatus shown in FIG. 1, (A) is a figure of the operation screen of the state before a measurement, (B) is after measurement It is a figure of the operation screen of a state. It is a flowchart for demonstrating operation | movement of the computer of the color measurement apparatus shown in FIG. It is a figure of an example of the measurement information recorded on the two-dimensional barcode of the card-type reagent shown in FIG. 2, (A) is a figure for demonstrating the whole measurement information and lot information, (B) is density | concentration information and absorbance It is a table | surface for demonstrating information. (A) is a graph for explaining the relationship between absorbance information and concentration information, and (B) is a diagram showing the relationship between absorbance information and concentration information as a linear function. It is a figure for demonstrating reading of captured image data, (A) is a figure which shows an example of the operation screen for a measurement folder reference, (B) is a figure which shows an example of the reference screen which opens a measurement folder, (C () Is a diagram showing an example of a reference screen for opening captured image data. It is a figure which shows an example of the operation screen on which the captured image data was displayed.

A color measurement device according to an embodiment of the present invention will be described with reference to the drawings.
As shown in FIG. 1, the color measurement device 10 puts blood, which is a sample, into a card-type reagent 100 used for thrombosis determination, analyzes the color reaction of the color reagent, and determines the D-dimer concentration. Is to measure. The color measurement device 10 includes a device main body 11 in which the card type reagent 100 is accommodated, and a computer 12 that controls the device main body 11.

As shown in FIG. 2, the card-type reagent 100 has a color reagent supported on a substantially rectangular base material 101.
The substrate 101 is formed with an administration unit 102 for administering blood and a measurement unit 103 for observing the color reaction of the color reagent by the administered blood from the outside. The substrate 101 is printed with optical reading information indicating measurement information regarding the color reaction of the color reagent. In the present embodiment, calibration curve information for calculating the D-dimer concentration is recorded by the two-dimensional barcode Q as measurement information related to the color reaction. For optically read information, printing with a laser marker is desirable because unevenness and blurring do not occur.
Further, a white pattern 104 and a black pattern 105 serving as a color reference are printed on the base material 101 as color reference reference patterns in order to accurately photograph the color reaction.
In addition, a region S where characters and symbols can be handwritten is provided on the base material 101. In the present embodiment, items such as “patient name”, “age”, and “MEMO” are preprinted so that doctors and nurses can fill in each item by handwriting.

Here, measurement information based on the two-dimensional barcode Q will be described with reference to FIG.
In the two-dimensional barcode Q, lot information, density information A to F and absorbance information A to F as calibration curve information, white pattern RGB information, and black pattern RGB information are recorded.
The lot information is information indicating the manufacturing lot number of the card type reagent 100. The absorbance information A to F is information for classifying the absorbance. The concentration information A to F is information indicating the concentration corresponding to the absorbance information A to F. The white pattern RGB information and the black pattern RGB information are RGB data indicating the color of a reference pattern (white pattern 104 and black pattern 105) for determining the shooting environment of the captured image data. The white pattern RGB information and the black pattern RGB information are obtained by measuring the colors of the white pattern 104 and the black pattern 105 when the card type reagent 100 is manufactured, and including the RGB data of this color in the two-dimensional barcode Q. Is.

As shown in FIG. 1, the apparatus main body 11 has a front surface formed in an arcuate surface, and is provided with a tray portion 11a in which the card type reagent 100 is set and stored so as to be freely inserted and removed.
As shown in FIG. 4, the apparatus main body 11 stores a storage sensor 113, an area sensor camera 114, an illumination unit 115, an input / output control unit 116, and an interface unit 117.

The storage sensor 113 detects that the tray unit 11 a is stored in the apparatus main body 11 and notifies the input / output control unit 116 of it.
As shown in FIG. 5, the area sensor camera 114 is arranged above the tray unit 11 a housed in the apparatus body 11, images the upper surface of the card type reagent 100 arranged on the tray unit 11 a, and uses the RGB data. The captured image data, which is a configured color image, is output to the input / output control unit 116. The RGB data constituting the captured image data, which is a color image, is data in which each color of red (R data), green (G data), and blue (B data) is expressed with 8-bit gradation. RGB data is provided for each pixel.
The illumination unit 115 is an annular light source in which LEDs are arranged in a circle around the outer periphery of the photographing lens around the photographing lens of the area sensor camera 114.
The illumination unit 115 is an LED in which chips each emitting red light, green light, and blue light are sealed, and a white LED that is white light by mixing three colors of light is used.
The white LED of the present embodiment is based on red light having a peak wavelength of 630 nm, green light of 520 nm, and blue light of 465 nm.

The input / output control unit 116 illustrated in FIG. 4 transmits captured image data from the area sensor camera 114 and storage state data from the storage sensor to the computer 12 via the interface unit 117.
The interface unit 117 is provided with a connector for performing communication between the input / output control unit 116 and the computer 12. In this embodiment, since communication is performed with the computer 12 by USB, a USB connector for communication is provided, and a USB connector for power supply is also provided in addition to the USB for communication.

The computer 12 functions as a part of the color measurement device 10 by operating a color measurement program. The computer 12 can be a notebook type or a desktop type.
As shown in FIG. 4, the computer 12 includes an interface unit 121, a display unit 122, an operation unit 123, a display control unit 124, an image processing unit 125, and a measurement value as a part of the color measurement device 10. A calculation unit 126, a time measurement unit 127, a setting unit 128, and a storage unit 129 are provided.

The interface unit 121 has a function of communicating with the apparatus main body 11. In the present embodiment, the USB is used in accordance with the apparatus main body 11.
The display unit 122 is a monitor on which captured image data from the area sensor camera 114, measured values, and operation buttons are displayed. The display unit 122 can be an LCD, an organic EL display, a CRT, or the like. The operation unit 123 is input means for instructing measurement. The operation unit 123 can be a keyboard, a mouse, a touch pad, a touch panel, or the like.

The display control unit 124 has a function for displaying information necessary for an operation by the inspector on the display unit 122 or notifying each unit of the operation when the inspector operates the operation unit 123 and presses an operation button. It has. In addition, the display control unit 124 has a function of reading captured image data stored in the storage unit 129 and displaying it on the display unit 122.
Based on the imaged image data of the card-type reagent 100 from the area sensor camera 114 obtained by outputting an imaging instruction to the area sensor camera 114, the image processing unit 125 displays the area where the two-dimensional barcode Q is imaged. In addition to reading, an area where a T (test) line indicating a color reaction is photographed is read. At this time, the image processing unit 125 performs a filter operation for extracting a color reaction on the RGB data of the region where the T (test) line is captured. Further, the image processing unit 125 performs color correction of the captured image data from the color correction data included in the measurement information.
Based on the measurement information indicated by the two-dimensional barcode Q read by the image processing unit 125, the measurement value calculation unit 126 calculates and outputs a measurement value corresponding to the color reaction indicated by the measurement unit 103.
The time measuring unit 127 instructs the measurement periodically after a predetermined time after instructing the measurement after a predetermined time since the tray unit 11a is stored in the apparatus main body 11.
The setting unit 128 has a function of performing various settings on the setting screen displayed on the display unit 122.
The storage unit 129 stores various settings and various measured data. The storage unit 129 can be a hard disk drive or a flash memory.

The operation and use state of the color measurement device 10 according to the embodiment of the present invention configured as described above will be described with reference to the drawings.
First, the inspector performs setting by the setting unit 128. In the setting screen W1 shown in FIG. 6, an input field is displayed so that measurement parameters and absorbance calculation parameters can be set. In addition, a “set” button BX for storing the value set in the input field in the storage unit 129 is displayed.
The measurement parameters can be set to a measurement interval during timer measurement and a timer measurement start time. In the present embodiment, the timer measurement measurement interval is set every 60 seconds, and the timer measurement start time (initial time) is set to 10 minutes.
In addition, as the absorbance calculation parameter, a weighting coefficient (absorbance coefficient) corresponding to each color of green, red, and blue can be set as the absorbance calculation parameter. In the present embodiment, green is set to 1 and others are set to 0.

Next, the examiner administers the sample to the administration unit 102 of the card type reagent 100 shown in FIG. Next, the inspector sets the card-type reagent 100 shown in FIG. 1 on the tray portion 11a of the apparatus main body 11, pushes the tray section 11a into the apparatus main body 11 and moves it horizontally, and moves the tray section 11a to the apparatus main body. 11.
When the tray unit 11a is stored in the apparatus main body 11, the storage sensor 113 shown in FIG.

In the computer 12, by operating the color measurement program, the display control unit 124 displays the operation screen W2 as shown in FIG. In the operation screen W2, an image display area Sx in which captured image data is displayed, a concentration display column R1 in which the D-dimer concentration is calculated and displayed from the measured absorbance, and an absorbance display column R2 in which the measured absorbance is displayed. A lot name column R3 for designating the location of the storage unit 129 of the computer 12 in which captured image data, measured absorbance, D-dimer concentration, and the like are stored is displayed.
In the lot name column R3, the inspector may input an arbitrary character string by operating the operation unit 123, or automatically display lot information when the two-dimensional barcode Q is read. You may make it designate.
In addition, the operation screen W2 displays a “normal measurement” button B1 that is an operation button for instructing the start of one measurement, and a “timer measurement” button B2 for performing measurement every predetermined time.

  The inspector operates the operation unit 123 of the computer 12 and presses the “normal measurement” button B1 on the operation screen W2 that can be pressed when the storage sensor 113 is in the storage state. When the “normal measurement” button B1 is pressed, the image processing unit 125 instructs the apparatus main body 11 to shoot through the interface unit 121 (see step S10 in FIG. 8). Further, the image processing unit 125 instructs the lighting unit 115 to be turned on.

  In the apparatus main body 11, a signal for instructing photographing is input to the input / output control unit 116 via the interface unit 117. When the imaging instruction output from the input / output control unit 116 is output to the area sensor camera 114, the area sensor camera 114 images the card-type reagent 100 set on the tray unit 11a, whereby the substrate 101 The imaged image data obtained by simultaneously capturing the two-dimensional barcode Q printed on the image and the measuring unit 103 capable of observing the color reaction generated by the color reagent can be photographed.

  The captured image data is output from the area sensor camera 114 to the input / output control unit 116. The captured image data is output from the input / output control unit 116 to the computer 12 via the interface unit 117.

In the computer 12, when the captured image data is input, the display control unit 124 displays the captured image data in the image display area Sx of the operation screen W2 (see step S20). A display example is shown in FIG.
Next, the image processing unit 125 reads each area captured in the captured image data. Since the card type reagent 100 is arranged at a predetermined photographing position of the area sensor camera 114, each region can be set in the image processing unit 125 in advance.

  For example, if the position of the base material 101 of the card type reagent 100 is determined in a state where the card type reagent 100 is set, the position where the two-dimensional barcode Q is printed can be defined as the known area S1. Also, the position where the C (control) line appears in the measurement unit 103 is somewhat different depending on the lot, but if the width is given as the known area S2, the C line will fit in the area S2, so that the area S2 Can be defined as Further, the position where the T (test) line in the measuring unit 103 appears is slightly different depending on the lot as in the case of the C line, but if the position of the C line is determined, the T line is separated from the C line by a predetermined distance. Therefore, the distance L from the C line farthest from the offset can be offset and the area S3 in front of the distance L can be defined.

  First, the image processing unit 125 reads RGB data of the white pattern 104 and the black pattern 105, which are color standards of the base material 101, from the captured image data (see step S30). Next, the image processing unit 125 reads the RGB data of the area S1 on which the two-dimensional barcode Q is printed (see step S40). No filter calculation is performed for the region S1. That is, the RGB data when the two-dimensional barcode Q is captured with white light from the illumination unit 115 in which red light, green light, and blue light are mixed is used as it is. The two-dimensional barcode Q composed of black and white blocks can clearly distinguish white and black by white light. Therefore, it is possible to accurately analyze the two-dimensional barcode Q by analyzing the RGB data as it is when the two-dimensional barcode Q is taken with white light.

The image processing unit 125 analyzes the two-dimensional barcode Q and obtains measurement information recorded in the two-dimensional barcode Q (see step S50). For example, measurement information as shown in FIG. 9A is recorded in the two-dimensional barcode Q. In the example shown in FIG. 9A, the first 10 bytes are lot information, which indicates the production lot number “5678901234”.
Next, the image processing unit 125 extracts white pattern RGB information and black pattern RGB information, which are color information of the reference pattern, from the measurement information (see step S60).

Next, the image processing unit 125 acquires the RGB data of the reference pattern (white pattern 104 and black pattern 105) read from the captured image data in step S30 and the RGB data of the reference pattern extracted from the measurement information in step S60 ( The white pattern RGB information and the black pattern RGB information) are respectively compared (see step S70).
Then, as a result of the comparison in step S70, if the difference is larger than the predetermined threshold, the image processing unit 125 displays an abnormality in the shooting environment on the display unit 122 (see step S80).
Assume that red is 8, green is 2, and blue is 4 in the RGB data of the white pattern 104 of the captured image data. Also, assume that the white pattern RGB information of the two-dimensional barcode Q is 2 for red, 1 for green, and 3 for blue. If the predetermined threshold is 5, the red difference is 6, which is a value exceeding the threshold, so the image processing unit 125 detects an abnormality.
Possible abnormalities in the shooting environment include insufficient illuminance and abnormalities in the area sensor camera 114. The inspector requests repair or adjustment when the display unit 122 displays a notification of abnormality in the imaging environment.

  As described above, the image processing unit 125 determines the shooting environment from the RGB data indicating the colors of the white pattern 104 and the black pattern 105 and the white pattern RGB information and the black pattern RGB information of the two-dimensional barcode Q. It is possible to prevent the measurement value corresponding to the color reaction from being calculated. In the present embodiment, an abnormality in the shooting environment is only detected and displayed on the display unit 122. However, the captured image data is corrected or the area sensor camera 114 is calibrated. Also good.

If the shooting environment is normal in step S70, the image processing unit 125 then reads the C line from the C line region S2 in the measurement unit 103 (see step S90).
Here, a method for measuring the absorbance of the T line will be described.
The image processing unit 125 reads the RGB data of the region S3, which is a range including the color reaction generated by the color reagent, and performs a filter operation of multiplying the RGB data by the weighting data set on the setting screen W1 (Step S1). S100).
In this embodiment, the color reagent is obtained by immunochromatography using colloidal gold particles. The particle diameter of the gold colloid particles used this time is about 20 to 80 nm, and the wavelength of the absorbance characteristic has a peak value in the vicinity of 520 to 530 nm. Therefore, the sensitivity of the color reaction is the highest when irradiated with green light that is absorbed by the color development in the color reaction. That is, since the green light is absorbed by the color developed by the color reaction and becomes black, it is easy to distinguish the color reaction from the surroundings, and the color reaction can be measured with high sensitivity. Therefore, as a filter operation, RGB data is multiplied by a coefficient weighted for each of RGB (red, green, and blue) in accordance with the wavelength range absorbed in the color development in the color reaction. If the color absorbed by the color development in the color reaction is green, the image processing unit 125 multiplies the RGB data by a weighting coefficient that makes the green value of RGB larger than other red and blue colors. For example, “0” is multiplied by red in the RGB data of the area sensor camera 114, “1” is multiplied by green, and “0” is multiplied by blue. By doing so, it is possible to perform measurement with the same sensitivity as when photographing with only green light. Although gold colloidal particles are used this time, it is possible to measure a color reagent using other particles by the above method.

The image processing unit 125 determines the presence or absence of a color reaction of the C line from the RGB data subjected to the filter calculation.
If a color reaction has occurred in the C line, the sample should have also passed through the T line, so the image processing unit 125 determines a region S3 within a predetermined distance range from the center of the C line, and has made a color reaction. Read T line. Then, the image processing unit 125 measures the absorbance of the T line (see step S110).

Next, the measurement value calculation unit 126 first determines in which range of the absorbance information A to F of the measurement information indicated by the two-dimensional barcode Q the absorbance from the image processing unit 125 is included.
Here, it is assumed that measurement information is obtained from a two-dimensional barcode Q as shown in FIG. For example, when the measured absorbance of the T line is 140, it is between the absorbance information E (160.00) and the absorbance information F (130.00). Accordingly, the D-dimer density, which is the measured value to be obtained, falls within the range of density information E (14.00) and density information F (12.00).

As shown in FIGS. 10A and 10B, when the absorbance is taken as the X axis and the concentration (D dimer concentration) is taken as the Y axis, it corresponds to the absorbance information A to F and the absorbance information A to F. Since the coordinates of the concentration information A to F are connected by a straight line (y = a1x + b1 to y = a5x + b5) represented by a linear function, any absorbance can be obtained by obtaining the slope and y intercept of the linear function from the coordinates of two points. The concentration (D dimer concentration) relative to (measured absorbance) can be determined.
For example, when the measured absorbance is 140, the coordinates of two points (x1, y1) = (160.00, 14.00), (x2, y2) = (130.00, 12.00) It can be seen that the slope is 1/15 and the y-intercept is 10/3.

  Therefore, the linear function is “D dimer concentration = 1/15 × absorbance + 10/3”. By substituting absorbance 140 into this equation, the D-dimer concentration “12.67” can be calculated. In this way, the D-dimer concentration is calculated by the measurement value calculation unit 126. By calculating the D dimer density, the display control unit 124 displays the D dimer density in the density display field R1 of the operation screen W2 as shown in FIG. 7B (see step S120). The measurement result is stored in the storage unit 129 together with the captured image data.

Next, timer measurement will be described based on the drawings.
The color reaction of the color reagent does not occur immediately after the sample is administered, but starts to react gradually after a predetermined time. For example, in the card type reagent 100 for measuring the D-dimer concentration, it takes about 7 minutes to about 10 minutes. Further, the color reaction proceeds with time in the color reagent.
The timer measurement can automatically measure the color reaction of such a color reagent.
The inspector sets the card type reagent 100 on the tray unit 11 a and stores it in the apparatus main body 11. Then, the inspector operates the operation unit 123 to press the “timer measurement” button B2 on the operation screen W2 shown in FIGS. 7A and 7B.

When the “timer measurement” button B2 on the operation screen W2 is pressed, the time measuring unit 127 starts measuring time. For example, the timer measurement start time is set to 10 minutes and the timer measurement time measurement interval is set to 60 seconds on the setting screen W1 shown in FIG. Then, the time measuring unit 127 waits for the timer measurement start time (initial time), about 10 minutes. When the initial time has elapsed, the time measuring unit 127 instructs the image processing unit 125 to perform the first measurement. When the end of the first measurement is notified from the measurement value calculation unit 126, the time measurement unit 127 then waits for the elapse of 60 seconds, the measurement interval during timer measurement. When the measurement interval at the time of timer measurement elapses, the time measuring unit 127 instructs the image processing unit 125 to perform the second measurement. From the second time on, an instruction to shoot is given at each measurement interval during timer measurement. The D-dimer concentration calculated by calculation is stored in the storage unit 129 together with the measured absorbance. The inspector can know the result by reading each D dimer density stored in the storage unit 129 through the operation screen displayed by the display control unit 124.
As described above, when the inspector sets the card type reagent 100 in the apparatus main body 11, it is not necessary to manually measure the initial time and the interval time, and the D-dimer concentration can be automatically measured. The measuring device 10 can perform inspection efficiently, and a busy nurse or the like can perform other tasks simultaneously without worrying about the measurement time.

As described above, according to the color measurement device 10 according to the present embodiment, the area sensor camera 114 simultaneously captures the two-dimensional barcode Q and the color reaction of the card type reagent 100, but the optical reading information is white light. The color reaction is read based on the RGB data, and the filter operation for extracting the color reaction is performed on the RGB data, so the optical reading information and the color reaction can be accurately obtained even in one shooting. Can be read. Therefore, the color measurement device 10 can easily manage the captured image data.
Further, since the filter operation simply multiplies the RGB data by the weighting coefficient, the color reaction can be accurately read with a simple process.

  Since the two-dimensional barcode Q and the color reaction of the card type reagent 100 are simultaneously photographed by the area sensor camera 114, only one area sensor camera 114 needs to be provided. Therefore, it is not necessary to separately provide a scanner for reading the two-dimensional barcode Q and an analysis unit for analyzing the color reaction of the card-type reagent 100, so that the cost of the color measurement device 10 can be suppressed. . Further, since the two-dimensional barcode Q and the color reaction of the card-type reagent 100 are photographed at the same time, the photographing of the two-dimensional barcode Q does not interfere with the photographing of the measuring unit 103. Therefore, the state of blood administered to the color reagent can be reliably read and accurately measured.

  Moreover, the space in the apparatus main body 11 can be effectively utilized rather than providing two units in the apparatus main body 11, and the size can be reduced. In addition, since the two-dimensional barcode Q and the color reaction of the card-type reagent 100 are simultaneously photographed, the photographing time can be shortened because only one photographing is required.

  Since the substrate 101 of the card type reagent 100 to be measured is positioned and then imaged by the area sensor camera 114, the area S1 of the two-dimensional barcode Q and the color reaction areas S2 and S3 of the card type reagent 100 are taken. Is defined by setting so that it is not necessary to search from captured image data using an image recognition technique, so that the burden of image processing can be reduced.

  When the area sensor camera 114 captures a C line or a T line, if a shadow is formed by the concave portion of the measurement unit 103, a color reaction cannot be accurately captured, but a circular shape is formed around the imaging lens of the area sensor camera 114. Since the illumination unit 115 in which the light source is arranged is provided, the influence of the shadow on the C line and the T line can be eliminated.

  The card-type reagent 100 is set on the tray unit 11a, stored in the apparatus main body 11, and photographed. Since the apparatus main body 11 is shielded and dark inside, and the same illumination light is always emitted from the illumination unit 115 during photographing, the card type reagent 100 can be photographed under the same conditions. Accordingly, since the captured image data is captured under uniform conditions, the measurement accuracy can be kept uniform.

Next, a case where the inspector reads out the captured image data captured by the area sensor camera 114 and performs confirmation will be described.
As shown in FIG. 11A, “Reference to measurement folder” is displayed by selecting “File” from the toolbar of the operation screen W2. The inspector operates the operation unit 123 to select “reference measurement folder”. By selecting “Measurement Folder Reference”, the display control unit 124 displays a reference screen for opening a “MeasurementData” folder that is a preset measurement folder.
In the reference screen W3 shown in FIG. 11B, the character string input in the lot name column R3 shown in FIG. 7A is displayed as the folder name. In the example shown in FIG. 11B, the production lot number “5678901234” which is lot information is the folder name.
When the inspector operates the operation unit 123 and selects the folder “5678901234” by double-clicking or the like, the display control unit 124 displays a reference screen in which the folder “5678901234” is opened.
In the reference screen W4 shown in FIG. 11C, an icon (thumbnail) of captured image data taken by normal measurement is displayed when the “normal measurement” button B1 shown in FIG. 7A is pressed.
When the inspector operates the operation unit 123 and selects the captured image data by double-clicking or the like, the display control unit 124 reads the captured image data from the storage unit 129 and displays the operation screen W2 as illustrated in FIG. The captured image data is displayed.

Since the captured image data thus captured can be read out from the storage unit 129 and confirmed at any time, past measurements can be confirmed again. In addition, the card-type reagent 100 is provided with a region S that can be handwritten. Therefore, an inspector such as a doctor or nurse reads out the captured image data and displays it on the display unit 122, thereby confirming handwritten information such as handwritten characters and symbols written in the card-type reagent 100 at any time. be able to.

In this embodiment, the optical reading information is a two-dimensional barcode, but it can be a one-dimensional barcode or a dot pattern.
In the present embodiment, the color measurement device 10 is configured as a separate body of the apparatus main body 11 and the computer 12, but the functions of each part of the computer 12 are mounted on the apparatus main body 11 and configured as a single unit. You may do it.
In addition, although the color reagent is a D-dimer concentration measurement reagent, the color measurement device 10 can measure other color reagent as long as it is a color reagent that undergoes a color reaction.

  Since the color reaction of the color reagent can be accurately measured, the present invention is suitable for hospitals, inspection institutions, educational institutions such as universities, and the like.

DESCRIPTION OF SYMBOLS 10 Color measurement apparatus 11 Apparatus main body 11a Tray part 113 Storage sensor 114 Area sensor camera 115 Illumination part 116 Input / output control part 117 Interface part 12 Computer 121 Interface part 122 Display part 123 Operation part 124 Display control part 125 Image processing part 126 Measurement Value calculation unit 127 Time measurement unit 128 Setting unit 129 Storage unit 100 Card type reagent 101 Base material 102 Administration unit 103 Measurement unit 104 White pattern 105 Black pattern Q Two-dimensional barcode S, S1 to S3 area W1 setting screen W2 operation screen Sx Image display area R1 Concentration display field R2 Absorbance display field R3 Lot name field L Distance W3, W4 Reference screen BX “Setting” button B1 “Normal measurement” button B2 “Timer measurement” button

Claims (6)

In a color measurement device for analyzing a color reaction (hereinafter referred to as a color reaction) in which a sample is administered and reacts with a color reagent carried on a substrate,
Optical reading information indicating measurement information related to the color reaction of the color reagent, and simultaneously photographing a range including the optical read information applied to the substrate and the color reaction generated by the color reagent. And an area sensor camera that outputs captured image data of a color image,
An illumination unit that illuminates a range including the optical reading information of the color reagent and a color reaction with white light mixed with three primary colors; and
Based on the imaged image data from the area sensor camera, the RGB data of the area where the optical reading information is photographed is read, and the RGB data of the area where the color reaction is photographed is read and presented to the RGB data. An image processing unit for performing a filter operation for extracting a color reaction;
A measurement value calculation unit that calculates and outputs a measurement value corresponding to the color reaction indicated by the RGB data subjected to the filter calculation based on the measurement information indicated by the optical reading information read by the image processing unit. Prepared ,
The image processing unit includes RGB data indicating a color of a reference pattern that is a color reference of the base material photographed in the captured image data, and RGB information indicating the RGB data of the reference pattern obtained from optical reading information If the difference is larger than a predetermined threshold value as a result of comparing the above, an abnormality in the photographing environment by the area sensor camera is displayed on the display unit.   2. The color measurement device according to claim 1, wherein the filter calculation by the image processing unit multiplies the RGB data by a coefficient weighted for each of RGB in accordance with a wavelength range absorbed by color development in a color reaction.   The color measurement apparatus according to claim 1 or 2, wherein an illumination unit having a light source arranged in a circular shape is provided around a photographing lens of the area sensor camera.   Time to measure the initial time at which the color reaction of the color reagent starts and output a shooting instruction to the image processing unit after elapse of time and output a shooting instruction to the image processing unit at every interval time after shooting The color measurement device according to any one of claims 1 to 3, wherein a measurement unit is provided.   Captured image data captured by the area sensor camera, including captured image data including handwritten information written on the surface of the substrate, is stored in the storage unit, and is read from the storage unit and displayed on the display unit. The color measurement device according to any one of claims 1 to 4, further comprising display control means for displaying. In a color measurement program for operating a computer as a color measurement device for analyzing a color reaction of an administered sample on a color reagent carried on a substrate,
The computer,
Optical reading information indicating measurement information related to the color reaction of the color reagent, and a range including the optical read information applied to the substrate and the color reaction generated by the color reagent Based on the color image captured image data from the area sensor camera that illuminates with the mixed white light and outputs it as captured image data, reads the RGB data of the area where the optical read information is captured. , An image processing unit that reads RGB data of a region where the color reaction is photographed and performs a filter operation to extract the color reaction to the RGB data;
Based on measurement information indicated by optical reading information read by the image processing unit, functions as a measurement value calculation unit that calculates and outputs a measurement value corresponding to the color reaction indicated by the RGB data subjected to the filter calculation then,
The image processing unit includes RGB data indicating a color of a reference pattern that is a color reference of the base material photographed in the captured image data, and RGB information indicating the RGB data of the reference pattern obtained from optical reading information result of comparing the door, if the difference is greater than a predetermined threshold value, color measurement program characterized you to view on a display unit an abnormality of the shooting environment by the area sensor camera.