JP6261996B2 - Varieties Identification Method for Gramineae Plants - Google Patents

Description

  The present invention relates to a rice variety-specific DNA marker and a method for identifying a variety using the same.

  In each stage from rice production to processing and distribution, a variety identification method using a DNA marker for scientific verification of the variety is indispensable.

  As rice variety identification methods developed so far, the RAPD-STS method (Patent Documents 1 and 2), the SNP method (Patent Documents 3 and 4), and the SSR method (Patent Document 5) are used for each analysis technique used. ~ 8) and the like. Non-patent document 1 describes a variety-specific DNA marker of Koshihikari.

JP 2001-095589 A Japanese Patent Laying-Open No. 2005-073655 JP 2004-248635 A JP 2009-219498 A Japanese Patent Laid-Open No. 10-057073 JP 2004-066511 JP 2007-037468 A JP 2012-055553 A

Kishine / Okunishi, Journal of Japan Society for Food Science and Technology, 58: 591-596 (2011)

  All of the conventional cultivar identification methods analyze a sample using several to several tens of DNA markers, and collate the obtained individual DNA types with the DNA types of the reference varieties obtained in advance. Have been identified. Therefore, (1) it is necessary to analyze several to several tens of DNA markers for each analysis sample, and it takes a lot of time and labor. (2) When a plurality of varieties are mixed in a sample, the obtained DNA type However, there is a problem that it is not matched with any standard varieties and cannot be verified. Non-Patent Document 1 merely discloses a variety-specific marker for Koshihikari, and no marker specific for other rice varieties has been known. Japanese rice varieties are very closely related, and it has been difficult to find a variety-specific marker. Therefore, development of a method capable of easily and specifically identifying rice varieties is demanded.

  An object of the present invention is to provide a genetic marker and a method for identifying varieties capable of accurately, simply and quickly performing various rice varieties.

The present invention provides the following inventions.
[1] A rice variety-specific marker which is at least one selected from the group consisting of the following polymorphisms (1) to (48) in the rice genome.
Polymorphism (1): Presence or absence of mPing between nucleotide residues 701 and 702 of the nucleotide sequence of SEQ ID NO: 61 Polymorphism (2): nucleotide residue of 673 of the nucleotide sequence of SEQ ID NO: 62 MPing polymorphism between group and 674 th nucleotide residue (3): mPing polymorphism between 639 th nucleotide residue and 640 th nucleotide residue in the nucleotide sequence of SEQ ID NO: 63 (4 ): Presence / absence polymorphism of mPing between the 730th nucleotide residue and the 731st nucleotide residue of the nucleotide sequence of SEQ ID NO: 64 (5): 692nd nucleotide residue and 693rd nucleotide of the nucleotide sequence of SEQ ID NO: 65 Polymorphism (6) of the presence or absence of mPing between nucleotide residues of: nucleotide 736 of the nucleotide sequence of SEQ ID NO: 66 MPing presence / absence polymorphism between the nucleotide residue and the 737th nucleotide residue (7): mPing presence / absence polymorphism between the 712nd nucleotide residue and the 713th nucleotide residue of the nucleotide sequence of SEQ ID NO: 67 (8): presence or absence of mPing between the 685th nucleotide residue and the 686th nucleotide residue of the nucleotide sequence of SEQ ID NO: 68 (9): the 738th nucleotide residue of the nucleotide sequence of SEQ ID NO: 69 Presence polymorphism (10) of mPing between the 739th nucleotide residues: Presence polymorphism (11) of mPing between the 733th nucleotide residue and the 734th nucleotide residue of the nucleotide sequence of SEQ ID NO: 70: M between the 713th nucleotide residue and the 714th nucleotide residue of the nucleotide sequence of SEQ ID NO: 71 ing presence / absence polymorphism (12): mPing presence / absence polymorphism between the 768th nucleotide residue and the 769th nucleotide residue of the nucleotide sequence of SEQ ID NO: 72 (13): 732th nucleotide of the nucleotide sequence of SEQ ID NO: 73 Polymorphism (14) of presence or absence of mPing between nucleotide residues 733 and 733: The presence or absence of mPing between nucleotide residues 531 and 532 of the nucleotide sequence of SEQ ID NO: 74 Type (15): Presence / absence of mPing between the 722nd and 723rd nucleotide residues of the nucleotide sequence of SEQ ID NO: 75 Polymorphism (16): 753rd nucleotide residue of the nucleotide sequence of SEQ ID NO: 76 And polymorphism (17) of mPing between nucleotide residues 754 and 754: nucleotide sequence of SEQ ID NO: 77 Presence / absence polymorphism (18) of mPing between the 727th and 728th nucleotide residues in the sequence: between the 716th and 717th nucleotide residues of the nucleotide sequence of SEQ ID NO: 78 Polymorphism with or without mPing (19): Polymorphism with or without mPing between the 632nd and 633th nucleotide residues of the nucleotide sequence of SEQ ID NO: 79 (20): 730th of the nucleotide sequence of SEQ ID NO: 80 Polymorphism (21) of presence or absence of mPing between the nucleotide residue of and the 731st nucleotide residue (21): presence or absence of mPing between the 686th nucleotide residue and the 687th nucleotide residue of the nucleotide sequence of SEQ ID NO: 81 Type (22): nucleotide residue 727 and nucleotide number 728 of the nucleotide sequence of SEQ ID NO: 82 Polymorphism with or without mPing between nucleotide residues (23): Polymorphism with or without mPing between nucleotide residues 803 and 804 of the nucleotide sequence of SEQ ID NO: 83 (24): SEQ ID NO: 84 Presence or absence of mPing between the 694th nucleotide residue and the 695th nucleotide residue of the nucleotide sequence (25): of the 728th nucleotide residue and the 729th nucleotide residue of the nucleotide sequence of SEQ ID NO: 85 MPing polymorphism (26): mPing polymorphism between the 656th nucleotide residue and the 657th nucleotide residue of the nucleotide sequence of SEQ ID NO: 86 (27): nucleotide sequence of SEQ ID NO: 87 Presence or absence of mPing between nucleotide residues 703 and 704 (28): Polymorphism of presence or absence of mPing between nucleotide residues 704 and 705 of the nucleotide sequence of SEQ ID NO: 88 (29): nucleotide residue 696 of the nucleotide sequence of SEQ ID NO: 89 Polymorphism of presence / absence of mPing between nucleotide residues 697 (30): Polymorphism of presence / absence of mPing between nucleotide residues 800 and 801 of the nucleotide sequence of SEQ ID NO: 90 (31): Presence / absence polymorphism of mPing between nucleotide residues 747 and 748 of the nucleotide sequence of SEQ ID NO: 91 (32): nucleotide residues 722 and 723 of nucleotide sequence of SEQ ID NO: 92 Presence / absence polymorphism of mPing between residues (33): 650th of the nucleotide sequence of SEQ ID NO: 93 Presence / absence polymorphism of mPing between nucleotide residue and 651st nucleotide residue (34): Presence / absence polymorphism of mPing between the 733th nucleotide residue and the 734th nucleotide residue of the nucleotide sequence of SEQ ID NO: 94 (35): Presence / absence polymorphism of mPing between the 578th and 579th nucleotide residues of the nucleotide sequence of SEQ ID NO: 95 (36): the 879th nucleotide residue of the nucleotide sequence of SEQ ID NO: 96 MPing presence / absence polymorphism between 880th nucleotide residues (37): mPing presence / absence polymorphism between the 718th nucleotide residue and the 719th nucleotide residue of the nucleotide sequence of SEQ ID NO: 97 (38): The 756th and 757th nucleotide residues of the nucleotide sequence of SEQ ID NO: 98 Whether mPing between polymorphisms (39): 689 th nucleotide residues and 69 0 th presence of mPing between nucleotide residues polymorphism (40) of the base sequence of SEQ ID NO: 99: nucleotide sequence of SEQ ID NO: 100 Polymorphism (41) of presence or absence of mPing between the 733th nucleotide residue and the 734th nucleotide residue of (n): mPing between the 729th nucleotide residue and the 730th nucleotide residue of the nucleotide sequence of SEQ ID NO: 101 Presence / absence polymorphism (42): polymorphism of presence or absence of mPing between nucleotide residues 705 and 706 of the nucleotide sequence of SEQ ID NO: 102 (43): nucleotide 603 of the nucleotide sequence of SEQ ID NO: 103 Polymorphism of presence or absence of mPing between nucleotide residue and nucleotide residue 604 (44 : Presence or absence of mPing between the 727th and 728th nucleotide residues of the nucleotide sequence of SEQ ID NO: 104 (45): the 734th and 735th nucleotide residues of the nucleotide sequence of SEQ ID NO: 105 Polymorphism of presence / absence of mPing between nucleotide residues (46): Presence / absence polymorphism of mPing between the 653rd nucleotide residue and the 654th nucleotide residue of the nucleotide sequence of SEQ ID NO: 106 (SEQ ID NO: 107) Presence / absence polymorphism (48) of mPing between the 726th nucleotide residue and the 727th nucleotide residue of the nucleotide sequence of: No. 626 and 627th nucleotide residue of the nucleotide sequence of SEQ ID NO: 108 Presence or absence of mPing between [2] any of polymorphisms (1) to (48) Is respectively, breed specific markers in rice according to [1].
Polymorphism (1): Presence / absence of mPing as nucleotide residues 702 to 1131 of the nucleotide sequence of SEQ ID NO: 1 Polymorphism (2): nucleotide residues 674 to 103 of the nucleotide sequence of SEQ ID NO: 2 Polymorphism of presence / absence of mPing insertion (3): Polymorphism of presence / absence of mPing which is the 640th to 1069th nucleotide residues of the nucleotide sequence of SEQ ID NO: 3 (4): nucleotide residues 731 to 1160 of the nucleotide sequence of SEQ ID NO: 4 Presence polymorphism of mPing as a group (5): Polymorphism polymorphism of mPing which is the 693-1122nd nucleotide residue of the nucleotide sequence of SEQ ID NO: 5 (6): 737-1166th nucleotide sequence of SEQ ID NO: 6 Presence / absence polymorphism of nucleotide residue mPing (7): mPing insertion site of nucleotide residues 713 to 1142 of the nucleotide sequence of SEQ ID NO: 7 No polymorphism (8): Presence / absence of mPing which is the 686 to 1115th nucleotide residue of the nucleotide sequence of SEQ ID NO: 8 Polymorphism (9): The 739 to 1168th nucleotide residue of the nucleotide sequence of SEQ ID NO: 9 Polymorphism of presence / absence of mPing insertion site (10): Presence / absence polymorphism of mPing which is the 734th to 1163rd nucleotide residues of the nucleotide sequence of SEQ ID NO: 10 (11): nucleotides 714 to 1143 of the nucleotide sequence of SEQ ID NO: 11 Presence / absence polymorphism of mPing insertion site which is a residue (12): Presence polymorphism of mPing which is the 769 to 1198th nucleotide residue of the nucleotide sequence of SEQ ID NO: 12 (13): 733 of the nucleotide sequence of SEQ ID NO: 13 Presence / absence polymorphism of mPing which is the 1162nd nucleotide residue (14): 532 to 961 nucleo of the nucleotide sequence of SEQ ID NO: 14 Polymorphism of insertion site of mPing which is a residue (15): polymorphism of presence or absence of mPing which is the nucleotide residues 723 to 1152 of the nucleotide sequence of SEQ ID NO: 15 (16): 754 of the nucleotide sequence of SEQ ID NO: 16 Presence / absence polymorphism of mPing which is ˜1183 nucleotide residue (17): Presence / absence polymorphism of mPing insertion site which is 728 to 1157th nucleotide residue of nucleotide sequence of SEQ ID NO: 17 (SEQ ID NO: 18) Presence / absence polymorphism of mPing as nucleotide residues 717 to 1146 of the nucleotide sequence (19): Presence / absence polymorphism of mPing as nucleotides 633 to 1062 of the nucleotide sequence of SEQ ID NO: 19 (20): SEQ ID NO: Presence / absence polymorphism of mPing which is the 731st to 1160th nucleotide residues of 20 nucleotide sequences (21): nucleotide sequence of SEQ ID NO: 21 Presence / absence polymorphism of mPing which is the 687 to 1116th nucleotide residue in the column (22): Presence / absence polymorphism of mPing which is the 728 to 1157th nucleotide residue of the nucleotide sequence of SEQ ID NO: 22 (23): SEQ ID NO: 23 Presence / absence polymorphism of mPing which is the 804 to 1233th nucleotide residue of the nucleotide sequence of (24): Presence polymorphism of mPing which is the 695 to 1124th nucleotide residue of the nucleotide sequence of SEQ ID NO: 24 (25): Sequence Presence / absence polymorphism of mPing which is the 729 to 1158th nucleotide residue of the nucleotide sequence of No. 25 (26): Presence / absence polymorphism of mPing which is the 657 to 1086th nucleotide residue of the nucleotide sequence of SEQ ID No. 26 (27) : Presence or absence polymorphism of mPing which is nucleotide residues 704 to 1133 of the nucleotide sequence of SEQ ID NO: 27 (28): Presence / absence polymorphism of mPing as nucleotide residues 705 to 1134 in the nucleotide sequence of column No. 28 (29): Presence / absence polymorphism of mPing as nucleotide residues 697 to 1126 in the nucleotide sequence of SEQ ID NO: 29 (30 ): Presence or absence polymorphism of mPing which is the 801 to 1230th nucleotide residue of the nucleotide sequence of SEQ ID NO: 30 (31): presence or absence polymorphism of mPing which is the 748 to 1177th nucleotide residue of the nucleotide sequence of SEQ ID NO: 31 (32): Presence / absence of mPing as nucleotides 723 to 1152 in the nucleotide sequence of SEQ ID NO: 32 (33): Presence / absence of mPing as nucleotides 651 to 1080 in the nucleotide sequence of SEQ ID NO: 33 Polymorphism (34): mPing which is a nucleotide residue at positions 734 to 1163 of the base sequence of SEQ ID NO: 34 Presence / absence polymorphism (35): mPing presence / absence polymorphism (36): nucleotide residues 580 to 1309 of the nucleotide sequence of SEQ ID NO: 36 Presence / absence polymorphism of mPing (37): Presence polymorphism of mPing which is the 732 to 1161st nucleotide residues of the nucleotide sequence of SEQ ID NO: 37 (38): Nucleotide residues of 757 to 1186 of the nucleotide sequence of SEQ ID NO: 38 MPing presence / absence polymorphism (39): mPing presence / absence polymorphism (40) which is the 690 to 1119th nucleotide residue of the nucleotide sequence of SEQ ID NO: 39: nucleotides 734 to 1163 of the nucleotide sequence of SEQ ID NO: 40 Presence / absence polymorphism of residue mPing (41): nucleotide residues 730 to 1159 in the nucleotide sequence of SEQ ID NO: 41 Presence / absence polymorphism of mPing as a group (42): polymorphism of presence or absence of mPing which is the nucleotide residue 706 to 1135 of the nucleotide sequence of SEQ ID NO: 42 (43): 604 to 1033 of the nucleotide sequence of SEQ ID NO: 43 Presence / absence polymorphism of nucleotide residue mPing (44): polymorphism of presence or absence of mPing as nucleotide residues 728 to 1157 in the nucleotide sequence of SEQ ID NO: 44 (45): 735 to 1164 of nucleotide sequence of SEQ ID NO: 45 MPing presence / absence polymorphism (46): nucleotide sequence 654 to 1083 of the nucleotide sequence mPing polymorphism (47): nucleotide sequence 727 of SEQ ID NO: 47 Presence / absence polymorphism (48) of mPing which is the 1156th nucleotide residue: 627 to 1056 of the nucleotide sequence of SEQ ID NO: 48 Eye whether mPing a nucleotide residues [3] [1] or a primer set composed of two or more primers capable of detecting the presence or absence of mPing in marker according to [2].
[4] The primer set according to [3], wherein the primer set is a PCR primer set or a LAMP primer set.
[5] The primer set according to [3] or [4] above, wherein the primer set is a primer consisting of the base sequence of SEQ ID NO: 59 and a primer consisting of the base sequence of SEQ ID NO: 60.
[6] The primer set is a primer consisting of the base sequence of SEQ ID NO: 51, a primer consisting of the base sequence of SEQ ID NO: 52, a primer consisting of the base sequence of SEQ ID NO: 53, a primer consisting of the base sequence of SEQ ID NO: 54, SEQ ID NO: 55 A primer set consisting of a primer consisting of the nucleotide sequence of SEQ ID NO: 56, a primer set consisting of a primer consisting of the base sequence of SEQ ID NO: 56, and / or a primer consisting of the base sequence of SEQ ID NO: 51, a primer consisting of the base sequence of SEQ ID NO: 57, and a sequence number [3] or [4], comprising a primer consisting of 53 base sequences, a primer consisting of the base sequence of SEQ ID NO: 58, a primer consisting of the base sequence of SEQ ID NO: 59, and a primer consisting of the base sequence of SEQ ID NO: 60 ] The primer set as described in.
[7] A rice variety identification probe set comprising at least one probe capable of detecting the presence or absence of mPing insertion in the marker according to [1] or [2].
[8] A detection kit for detecting the marker according to [1], comprising the primer set according to any one of [3] to [6] or the probe according to [6].
[9] A method for identifying a variety of gramineous plants, comprising confirming the presence or absence of mPing insertion in the marker according to [1] or [2] with respect to the rice genome contained in the sample.
[10] The method according to [9] above, wherein any one of the following steps is performed.
Step [1]: When mPing is inserted in the polymorphism (1), it is determined that the test subject is at a glance or tender. Step [2]: mPing is inserted in the polymorphism (2) If the mPing is not inserted in the polymorphism (6), (12), (25) or (31), (36) or (37), the test subject is determined to be Akitakomachi Step [3]: When mPing is inserted in polymorphism (3), and mPing is not inserted in polymorphism (6), (10) or (11), and (15), It is determined that the test subject is Hinohikari or Kuma no Mori [4]: When mPing is inserted in polymorphism (4) or (5), the test subject is Nanatsuboshi Judgment [5]: When mPing is inserted in the polymorphisms (2) and (6), it is determined that the test subject is an eclipse [6]: mPing in the polymorphisms (7) and (8) Step 7 to determine that the test subject is Kirara 397 when mPing is inserted [7]: mPing is inserted in polymorphism (9), and mPing is not inserted in polymorphism (43) In this case, the step [8] of determining that the test subject is a straight line: When mPing is inserted in the polymorphism (10) or (11) and no mPing is inserted in the polymorphism (3) The step [9] of determining that the test subject is a fragile splash is determined when the mPing is inserted in the polymorphisms (2) and (12), and the test subject is determined to be a romantic. Step [10]: When mPing is inserted in the polymorphism (13), it is determined that the test subject is Kinuhikari [11]: When mPing is inserted in the polymorphism (14) In addition, it is determined that the test subject is a dream of Asahi [12]: When mPing is inserted in polymorphisms (8) and (15), it is determined that the test subject is dreamy. Step [13]: When mPing is inserted in the polymorphism (16), it is determined that the test object is fungus [14]: mPing in the polymorphism (17) or (18) Step 15 to determine that the test subject is a glossy princess when the mPing is inserted in the polymorphism (19). Determining step [16]: a step of determining that the test subject is a dream sushi when mPing is inserted in the polymorphism (20) and no mPing is inserted in the polymorphism (12) [ 17]: When the mPing is inserted in the polymorphism (21) and the mPing is not inserted in the polymorphism (19), it is determined that the test subject is a diaper or a hashimo [18]: When mPing is inserted in the polymorphism (22) or (23), determining that the test subject is fluffy [19]: polymorphism (7) , (15), ( 24), when mPing is inserted, it is determined that the subject to be tested is a dream [20]: mPing is inserted in polymorphism (25), and polymorphism (2) And (9) when mPing is not inserted, determining that the test subject is unsightly [21]: When mPing is inserted in polymorphism (26), the test subject is Step [22] for determining that it is a kinmusume step [23]: When mPing is inserted in the polymorphism (27), a step [23] for determining that the test subject is Aichi Kaori [28] ) Or (29), when mPing is inserted, determining that the test subject is rough [24]: When mPing is inserted in polymorphism (30), the test subject is Step [25] of determining that it is a dream Mizuho: Step [26] of determining that the subject to be tested is Sasanishiki when mPing is inserted in the polymorphism (31). When the mPing is inserted in the polymorphism (32), determining that the test subject is a milky queen [27]: When the mPing is inserted in the polymorphism (33) or (34) The step of determining that the test subject is a chickworm [28]: mPing is inserted in the polymorphism (35), and mPing is inserted in the polymorphism (12) or (38) and (39) or (40). In the case where no P is inserted, it is determined that the test subject is Himenomochi, Manamusume or Chiyonishiki [29]: When mPing is inserted in the polymorphism (36) or (37), the test is performed. target step [30] to determine that the rice Kogane is: polymorphisms (3) or (15), when the mPing is inserted in (38), A step of determining that the test object is a child of wind [31]: a step of determining that the test target is a cottonhead when mPing is inserted in the polymorphism (39) or (40) [32] : When mPing is inserted in the polymorphism (41) or (42), it is determined that the test subject is squirting [33]: mPing is inserted in the polymorphism (43) If the polymorphism (45) does not have mPing inserted, it is determined that the subject to be tested is a step [34]: when the polymorphism (44) has mPing inserted, Step [35] for determining that the test object is a kitemochi: When the mPing is inserted in the polymorphism (45), it is determined that the test target is a bare bran [36]: When mPing is inserted in the polymorphism (46) or (47), determining that the subject is Yamada Nishiki [37]: mPing is inserted in the polymorphism (48) Step [38]: When mPing is inserted in polymorphisms (35) and (38), the test subject is stuck. Determining that there is a step [39]: When mPing is inserted in polymorphism (3) and (6), (10) or (11), the test object is a Yukinko dance Determining step [40]: When mPing is inserted in polymorphisms (12) and (20), determinating that the test subject is a light beam [41]: mPing in polymorphism (12) Is inserted, polymorphism (2), (1 3), (20), (26), and (35) or (38), when mPing is not inserted, it is determined that the test object is clear [42]: Many When mPing is not inserted in any of the types (1) to (48), the test subjects are Koshihikari, Akebono, Yume Obako, Nihonbare, Aya no Kagayaki, Yume Shizuku, Sabiri, Autumn poetry, Judgment or judgment of Asahi.
[11] Using the primer set according to any one of [3] to [6], the probe according to [7], or the kit according to [8], the above [1] or [ The method for identifying a variety according to [9] or [10], comprising confirming the presence or absence of insertion of mPing in the marker according to [2].

  According to the rice cultivar-specific marker and cultivar identification method of the present invention, rice cultivar identification can be performed accurately, simply and quickly.

FIG. 1 is a diagram showing a corresponding site in a pupil where mPing is inserted in polymorphism (1) and its surrounding base sequence (SEQ ID NO: 1). In FIG. 1, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 2 is a diagram showing a corresponding site in Akitakomachi where mPing is inserted in the polymorphism (2) and its surrounding base sequence (SEQ ID NO: 2). In FIG. 2, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 3 is a diagram showing a corresponding site in Hinokikari in which mPing is inserted in polymorphism (3) and its surrounding base sequence (SEQ ID NO: 3). In FIG. 3, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 4 is a diagram showing a corresponding site in Nanatsubo where mPing is inserted in the polymorphism (4) and its surrounding base sequence (SEQ ID NO: 4). In FIG. 4, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 5 is a diagram showing a corresponding site in Nanatsubo where mPing is inserted in the polymorphism (5) and the base sequence around it (SEQ ID NO: 5). In FIG. 5, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the enclosed line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 6 is a diagram showing a corresponding site in a gutter in which mPing is inserted in the polymorphism (6) and the surrounding base sequence (SEQ ID NO: 6). In FIG. 6, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 7 is a diagram showing the corresponding site in Kirara 397 in which mPing is inserted in the polymorphism (7) and the base sequence around it (SEQ ID NO: 7). In FIG. 7, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 8 is a diagram showing the corresponding site in Kirara 397 in which mPing is inserted in the polymorphism (8) and the surrounding base sequence (SEQ ID NO: 8). In FIG. 8, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 9 is a diagram showing a corresponding site in makugure in which mPing is inserted in the polymorphism (9) and its surrounding base sequence (SEQ ID NO: 9). In FIG. 9, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 10 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 10) in a delicate pattern in which mPing is inserted in the polymorphism (10). In FIG. 10, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 11 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 11) in a delicate pattern in which mPing is inserted in the polymorphism (11). In FIG. 11, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 12 is a diagram showing a corresponding site in the romance in which mPing is inserted in the polymorphism (12) and its surrounding base sequence (SEQ ID NO: 12). In FIG. 12, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 13: is a figure which shows the corresponding part in Kinuhikari in which mPing is inserted in polymorphism (13), and its base sequence (sequence number 13). In FIG. 13, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 14 is a diagram showing a corresponding site in Asahi's dream in which mPing is inserted in the polymorphism (14) and its surrounding base sequence (SEQ ID NO: 14). In FIG. 14, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 15 is a diagram showing a corresponding site and its surrounding base sequence (SEQ ID NO: 15) in Yumepirika in which mPing is inserted in the polymorphism (15). In FIG. 15, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 16 is a diagram showing the corresponding site and the surrounding base sequence (SEQ ID NO: 16) in fusago glasses in which mPing is inserted in the polymorphism (16). In FIG. 16, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 17 is a diagram showing a corresponding site and its surrounding base sequence (SEQ ID NO: 17) in Tsutsuhime where mPing is inserted in the polymorphism (17). In FIG. 17, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 18 is a diagram showing a corresponding site and its surrounding base sequence (SEQ ID NO: 18) in Tsutsuhime where mPing is inserted in the polymorphism (18). In FIG. 18, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 19 is a diagram showing a corresponding site in Hanaechizen into which mPing is inserted in the polymorphism (19) and the base sequence around it (SEQ ID NO: 19). In FIG. 19, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 20 is a view showing a corresponding site and a base sequence (SEQ ID NO: 20) in the dream in which mPing is inserted in the polymorphism (20). In FIG. 20, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence replicated when mPing is inserted. FIG. 21 is a diagram showing a corresponding site and a base sequence (SEQ ID NO: 21) in the vicinity of the puppy where mPing is inserted in the polymorphism (21). In FIG. 21, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the enclosed line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 22 is a diagram showing a corresponding site and a base sequence in the vicinity thereof (SEQ ID NO: 22) in the furkinko in which mPing is inserted in the polymorphism (22). In FIG. 22, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 23 is a diagram showing a corresponding site and a peripheral nucleotide sequence (SEQ ID NO: 23) in the furkinko in which mPing is inserted in the polymorphism (23). In FIG. 23, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 24 is a diagram showing a corresponding site in Hoshino Yume in which mPing is inserted in the polymorphism (24) and its surrounding base sequence (SEQ ID NO: 24). In FIG. 24, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 25 is a diagram showing a corresponding site and its surrounding nucleotide sequence (SEQ ID NO: 25) in a mental disorder in which mPing is inserted in the polymorphism (25). In FIG. 25, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 26 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 26) in a silkworm where mPing is inserted in the polymorphism (26). In FIG. 26, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 27 is a diagram showing a corresponding site and a peripheral nucleotide sequence (SEQ ID NO: 27) in the cage of Aichi where mPing is inserted in the polymorphism (27). In FIG. 27, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 28 is a diagram showing a corresponding site and a base sequence in the vicinity thereof (SEQ ID NO: 28) in the roughing in which mPing is inserted in the polymorphism (28). In FIG. 28, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 29 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 29) in the roughing in which mPing is inserted in the polymorphism (29). In FIG. 29, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 30 is a diagram showing a corresponding site in Yume Mizuho in which mPing is inserted in the polymorphism (30) and its surrounding base sequence (SEQ ID NO: 30). In FIG. 30, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 31 is a diagram showing a corresponding site in Sasanishiki in which mPing is inserted in the polymorphism (31) and its surrounding base sequence (SEQ ID NO: 31). In FIG. 31, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 32 is a diagram showing a corresponding site in a milky queen in which mPing is inserted in the polymorphism (32) and its surrounding base sequence (SEQ ID NO: 32). In FIG. 32, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 33 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 33) in a chickweed in which mPing is inserted in the polymorphism (33). In FIG. 33, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 34 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 34) in a chickweed in which mPing is inserted in the polymorphism (34). In FIG. 34, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 35 is a diagram showing a corresponding site in Hymenomochi where mPing is inserted in the polymorphism (35) and the base sequence around it (SEQ ID NO: 35). In FIG. 35, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 36 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 36) in a rice cake in which mPing is inserted in the polymorphism (36). In FIG. 36, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 37 is a diagram showing the corresponding site and the surrounding base sequence (SEQ ID NO: 37) in the kokomochi in which mPing is inserted in the polymorphism (37). In FIG. 37, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 38 is a diagram showing a corresponding site and a base sequence (SEQ ID NO: 38) in the vicinity of a wind pup that has mPing inserted in the polymorphism (38). In FIG. 38, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 39 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 39) in cotton bud in which mPing is inserted in the polymorphism (39). In FIG. 39, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 40 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 40) in cotton bud in which mPing is inserted in the polymorphism (40). In FIG. 40, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 41 is a diagram showing a corresponding site and a base sequence in the vicinity thereof (SEQ ID NO: 41) in a rice cake where mPing is inserted in the polymorphism (41). In FIG. 41, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 42 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 42) in a rice cake where mPing is inserted in the polymorphism (42). In FIG. 42, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 43 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 43) in Tatsukomochi in which mPing is inserted in the polymorphism (43). In FIG. 43, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 44 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 44) in Yukimochi where mPing has been inserted in the polymorphism (44). In FIG. 44, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 45 is a diagram showing a corresponding site in a kinu hat where mPing is inserted in the polymorphism (45) and a base sequence in the vicinity thereof (SEQ ID NO: 45). In FIG. 45, the underlined portion indicates the mPing insertion site, and the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted. FIG. 46 is a diagram showing a corresponding site in Yamada Nishiki in which mPing is inserted in the polymorphism (46) and its surrounding base sequence (SEQ ID NO: 46). In FIG. 46, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the enclosed line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 47 is a diagram showing a corresponding site in Yamada Nishiki in which mPing is inserted in the polymorphism (47) and its surrounding base sequence (SEQ ID NO: 47). In FIG. 47, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is duplicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 48 is a diagram showing a corresponding site and base sequence (SEQ ID NO: 48) in Hyakumangoku in which mPing is inserted in the polymorphism (48). In FIG. 48, the underlined portion indicates the mPing insertion site, the double underlined portion indicates a repetitive sequence that is replicated when mPing is inserted, and the encircled line indicates a base that is different from the common base sequence of mPing or the base sequence of the homologous region in Nipponbare. Show. FIG. 49 is a diagram showing an outline of various regions necessary for designing a LAMP primer set. FIG. 50 is a diagram showing an outline of two or more primers for detecting the presence or absence of mPing insertion. FIG. 51 is a diagram showing a determination example using the difference in the amplified base length due to the PCR reaction. FIG. 52 is a diagram showing a determination example using the fluorescence coloring of the LAMP reaction solution. FIG. 53 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (1) and its surrounding base sequence (SEQ ID NO: 61). In FIG. 53, the double underlined portion indicates the mPing insertion site. FIG. 54 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (2) and the surrounding base sequence (SEQ ID NO: 62). In FIG. 54, the double underlined portion indicates the mPing insertion site. FIG. 55 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (3) and its surrounding base sequence (SEQ ID NO: 63). In FIG. 55, the double underlined portion indicates the mPing insertion site. FIG. 56 is a diagram showing a corresponding site in Nipponbare where mPing is not inserted in the polymorphism (4) and the base sequence in the vicinity thereof (SEQ ID NO: 64). In FIG. 56, the double underlined portion indicates the mPing insertion site. FIG. 57 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (5) and the surrounding base sequence (SEQ ID NO: 65). In FIG. 57, the double underlined portion indicates the mPing insertion site. FIG. 58 is a diagram showing a corresponding site and its surrounding base sequence (SEQ ID NO: 66) in Nipponbare where mPing is not inserted in polymorphism (6). In FIG. 58, the double underlined portion indicates the mPing insertion site. FIG. 59 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (7) and the surrounding base sequence (SEQ ID NO: 67). In FIG. 59, the double underlined portion indicates the mPing insertion site. FIG. 60 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (8) and the surrounding base sequence (SEQ ID NO: 68). In FIG. 60, the double underlined portion indicates the mPing insertion site. FIG. 61 is a view showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (9) and the surrounding base sequence (SEQ ID NO: 69). In FIG. 61, the double underlined portion indicates the mPing insertion site. FIG. 62 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (10) and its surrounding base sequence (SEQ ID NO: 70). In FIG. 62, the double underlined portion indicates the mPing insertion site. FIG. 63 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (11) and its surrounding base sequence (SEQ ID NO: 71). In FIG. 63, the double underlined portion indicates the mPing insertion site. FIG. 64 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (12) and its surrounding base sequence (SEQ ID NO: 72). In FIG. 64, the double underlined portion indicates the mPing insertion site. FIG. 65 is a diagram showing the corresponding site in Nipponbare where mPing is not inserted in the polymorphism (13) and the base sequence in the vicinity thereof (SEQ ID NO: 73). In FIG. 65, the double underlined portion indicates the mPing insertion site. FIG. 66 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (14) and the base sequence around it (SEQ ID NO: 74). In FIG. 66, the double underlined portion indicates the mPing insertion site. FIG. 67 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (15) and its surrounding base sequence (SEQ ID NO: 75). In FIG. 67, the double underlined portion indicates the mPing insertion site. FIG. 68 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (16) and its surrounding base sequence (SEQ ID NO: 76). In FIG. 68, the double underlined portion indicates the mPing insertion site. FIG. 69 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (17) and its surrounding base sequence (SEQ ID NO: 77). In FIG. 69, the double underlined portion indicates the mPing insertion site. FIG. 70 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (18) and its surrounding base sequence (SEQ ID NO: 78). In FIG. 70, the double underlined portion indicates the mPing insertion site. FIG. 71 is a diagram showing a corresponding site in Hanaechizen into which mPing is inserted in the polymorphism (19) and its surrounding base sequence (SEQ ID NO: 79). In FIG. 71, the double underlined portion indicates the mPing insertion site. FIG. 72 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (20) and the surrounding base sequence (SEQ ID NO: 80). In FIG. 72, the double underlined portion indicates the mPing insertion site. FIG. 73 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (21) and its surrounding base sequence (SEQ ID NO: 81). In FIG. 73, the double underlined portion indicates the mPing insertion site. FIG. 74 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (22) and its surrounding base sequence (SEQ ID NO: 82). In FIG. 74, the double underlined portion indicates the mPing insertion site. FIG. 75 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (23) and its surrounding base sequence (SEQ ID NO: 83). In FIG. 75, the double underlined portion indicates the mPing insertion site. FIG. 76 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (24) and its surrounding base sequence (SEQ ID NO: 84). In FIG. 76, the double underlined portion indicates the mPing insertion site. FIG. 77 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (25) and its surrounding base sequence (SEQ ID NO: 85). In FIG. 77, the double underlined portion indicates the mPing insertion site. FIG. 78 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (26) and the surrounding base sequence (SEQ ID NO: 86). In FIG. 78, the double underlined portion indicates the mPing insertion site. FIG. 79 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (27) and its surrounding base sequence (SEQ ID NO: 87). In FIG. 79, the double underlined portion indicates the mPing insertion site. FIG. 80 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (28) and its surrounding base sequence (SEQ ID NO: 88). In FIG. 80, the double underlined portion indicates the mPing insertion site. FIG. 81 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (29) and its surrounding base sequence (SEQ ID NO: 89). In FIG. 81, the double underlined portion indicates the mPing insertion site. FIG. 82 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (30) and its surrounding base sequence (SEQ ID NO: 90). In FIG. 82, the double underlined portion indicates the mPing insertion site. FIG. 83 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (31) and the surrounding base sequence (SEQ ID NO: 91). In FIG. 83, the double underlined portion indicates the mPing insertion site. FIG. 84 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (32) and the surrounding base sequence (SEQ ID NO: 92). In FIG. 84, the double underlined portion indicates the mPing insertion site. FIG. 85 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (33) and the surrounding base sequence (SEQ ID NO: 93). In FIG. 85, the double underlined portion indicates the mPing insertion site. FIG. 86 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (34) and its surrounding base sequence (SEQ ID NO: 94). In FIG. 86, the double underlined portion indicates the mPing insertion site. FIG. 87 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (35) and its surrounding base sequence (SEQ ID NO: 95). In FIG. 87, the double underlined portion indicates the mPing insertion site. FIG. 88 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (36) and its surrounding base sequence (SEQ ID NO: 96). In FIG. 88, the double underlined portion indicates the mPing insertion site. FIG. 89 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (37) and its surrounding base sequence (SEQ ID NO: 97). In FIG. 89, the double underlined portion indicates the mPing insertion site. FIG. 90 is a diagram showing a corresponding site and a base sequence (SEQ ID NO: 98) in the vicinity of a wind pup that has mPing inserted in the polymorphism (38). In FIG. 90, the double underlined portion indicates the mPing insertion site. FIG. 91 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (39) and its surrounding base sequence (SEQ ID NO: 99). In FIG. 91, the double underlined portion indicates the mPing insertion site. FIG. 92 is a diagram showing a corresponding site and a surrounding nucleotide sequence (SEQ ID NO: 100) in cotton bud in which mPing is inserted in the polymorphism (40). In FIG. 92, the double underlined portion indicates the mPing insertion site. FIG. 93 is a view showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (41) and its surrounding base sequence (SEQ ID NO: 101). In FIG. 93, the double underlined portion indicates the mPing insertion site. FIG. 94 is a view showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (42) and its surrounding base sequence (SEQ ID NO: 102). In FIG. 94, the double underlined portion indicates the mPing insertion site. FIG. 95 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (43) and its surrounding base sequence (SEQ ID NO: 103). In FIG. 95, the double underlined portion indicates the mPing insertion site. FIG. 96 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (44) and its surrounding base sequence (SEQ ID NO: 104). In FIG. 96, the double underlined portion indicates the mPing insertion site. FIG. 97 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (45) and the surrounding base sequence (SEQ ID NO: 105). In FIG. 97, the double underlined portion indicates the mPing insertion site. FIG. 98 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (46) and its surrounding base sequence (SEQ ID NO: 106). In FIG. 98, the double underlined portion indicates the mPing insertion site. FIG. 99 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (47) and the surrounding base sequence (SEQ ID NO: 107). In FIG. 99, the double underlined portion indicates the mPing insertion site. FIG. 100 is a diagram showing a corresponding site in Nipponbare in which mPing is not inserted in the polymorphism (48) and the surrounding base sequence (SEQ ID NO: 108). In FIG. 100, the double underlined portion indicates the mPing insertion site.

  The present invention provides rice variety-specific markers and methods for identifying rice varieties using the same.

  The cultivar-specific marker of the present invention is a marker based on the presence or absence of mPing insertion found when comparing the genome sequence of rice cultivar Nipponbare with the genome sequence of other varieties. It is a marker that can be used. mPing is a kind of transposon. mPing is a 430 bp polynucleotide. When mPing is inserted into the genome, TAA or TTA at the insertion site is duplicated and held at both ends of the insertion site. Therefore, when there is insertion, it becomes longer (430 bp + 3) bp (for example, see below).

  The mPing to be inserted may be an equivalent arrangement of mPing. An equivalent sequence of mPing is a part of its bases (for example, within 80, within 70, within 60, within 50, within 40, within 30, within 20 and within 10, preferably Is 5 or less, more preferably 4 or less, still more preferably 3 or less, even more preferably 2 or less, and most preferably 1) to a mutation (eg, deletion, substitution) , Addition, inversion, and insertion) (see, for example, the underlined portions in FIGS. 1, 5, 13, 14, 19, 21, 29, 35, 39, 41, 43, 44, 46, and 48) . Not only the polymorphisms shown in these figures but also all of the polymorphisms (1) to (48), an equivalent arrangement of mPing may be inserted.

  The variety-specific marker of the present invention is at least one selected from the following polymorphisms (1) to (48) in the rice genome.

Polymorphism (1): Presence or absence of mPing between the 701st nucleotide residue and the 702th nucleotide residue of the nucleotide sequence of SEQ ID NO: 61, that is, the nucleotide residues 702 to 1131 of the nucleotide sequence of SEQ ID NO: 1 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 61 is the nucleotide sequence of nucleotide residues 27774322 to 27775778 of Nipponbare Chromosome 3, and SEQ ID NO: 1 is a convoluted sequence corresponding to the same part. The insertion site of mPing in the polymorphism (1) is between positions 701 and 702 of SEQ ID NO: 61. When mPing is inserted, the portion of nucleotide residues 702 to 1131 of SEQ ID NO: 1 is mPing It corresponds to the insertion site (underlined part in FIG. 1). The insertion site of mPing in the polymorphism (1) (between the 701st nucleotide residue and the 702th nucleotide residue of the nucleotide sequence of SEQ ID NO: 61) is located at the 27th, 775th, 022nd positions of chromosome 3 in Nipponbare. Corresponds between the nucleotide residue and the 27,775,023rd nucleotide residue (Table 1). In the glimpse, mPing is inserted in the polymorphism (1) (SEQ ID NO: 1, FIG. 1), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 61, FIG. 53). In the polymorphism (1), mPing is inserted at the same site as that of the eyelid even in the case of the ladybug.

  Nipponbare is a standard variety of Japanese rice, and its entire genome sequence has been decoded and published. The complete genome sequence of Nipponbare can be obtained from Os-Nipponballe-Reference-IRGSP-1.0 (IRGSP-1.0) (http://rapdb.dna.affrc.go.jp). Genome sequence information is based on information in IRGSP-1.0. The base sequence of the corresponding site of Nipponbare does not necessarily match the base sequence of the corresponding site of varieties other than Nipponbare, and may contain mutations.

Polymorphism (2): Presence or absence of mPing between the 673th nucleotide residue and the 674th nucleotide residue of the base sequence of SEQ ID NO: 62, that is, the 674 to 1103rd nucleotide residues of the base sequence of SEQ ID NO: 2 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 62 is the nucleotide sequence of nucleotide residues 7966660 to 7968144 of Nipponbare Chromosome 3, and SEQ ID NO: 2 is an Akitakomachi sequence corresponding to the same part. The insertion site of mPing in the polymorphism (2) is between the 673th and 674th positions of SEQ ID NO: 62. When mPing is inserted, the portion of nucleotide residues 674 to 1103 of SEQ ID NO: 2 is mPing It corresponds to the insertion site (underlined part in FIG. 2). The insertion site of mPing in the polymorphism (2) (between the 673th nucleotide residue and the 674th nucleotide residue of the nucleotide sequence of SEQ ID NO: 62) is the 7th, 967th, 332nd position of chromosome 3 in Nipponbare Corresponds between the nucleotide residue and the 7,967,333th nucleotide residue (Table 1). In Akitakomachi, mPing is inserted in the polymorphism (2) (SEQ ID NO: 2, FIG. 2), whereas mPing is not inserted in Nipponbare (SEQ ID NO: 62, FIG. 54). In polymorphism (2), mPing is inserted at the same site as Akitakomachi even in Haenuki, Tsugaru Roman, Sasanishiki, and Kokomochi.

Polymorphism (3): presence or absence of mPing between the 639th nucleotide residue and the 640th nucleotide residue of the nucleotide sequence of SEQ ID NO: 63, that is, the 640th to 1069th nucleotide residues of the nucleotide sequence of SEQ ID NO: 3 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 63 is the nucleotide sequence of nucleotide residues from the 12,523,354th to the 12,524,675th nucleotide of the third chromosome of Nipponbare, and SEQ ID NO: 3 corresponds to the same part Hino Hikari array. The insertion site of mPing in the polymorphism (3) is between the 639th and 640th positions of SEQ ID NO: 63, and when mPing is inserted, the portion of nucleotide residues 640 to 1069 of SEQ ID NO: 3 is mPing It corresponds to the insertion site (underlined part in FIG. 3). The insertion site of mPing in the polymorphism (3) (between the 639th nucleotide residue and the 640th nucleotide residue of the nucleotide sequence of SEQ ID NO: 63) is located at the 12th, 523th, and 992nd positions of chromosome 3 in Nipponbare. It corresponds to the nucleotide residue and the 12,523,993th nucleotide residue (Table 1). In Hinohikari, mPing is inserted in the polymorphism (3) (SEQ ID NO: 3, FIG. 3), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 63, FIG. 55). In polymorphism (3), mPing is inserted at the same site as Hinohikari in Yukiniko Mai, Kuma no Mori, Kaze no Komochi, Hachichochimochi and Kitayukimochi.

Polymorphism (4): Presence or absence of mPing between the 730th nucleotide residue and the 731st nucleotide residue of the nucleotide sequence of SEQ ID NO: 64, that is, the 731st to 1160th nucleotide residues of the nucleotide sequence of SEQ ID NO: 4 The presence or absence of mPing is the insertion site of mPing in polymorphism (4) is between 730 and 731 of SEQ ID NO: 64, and when mPing is inserted, nucleotide residues 731 to 1160 of SEQ ID NO: 4 are left. The base portion corresponds to the mPing insertion site (underlined portion in FIG. 4). The insertion site of mPing in the polymorphism (4) (between the 730th nucleotide residue and the 731st nucleotide residue of the nucleotide sequence of SEQ ID NO: 64) is located at the 16th, 937, 071th position of chromosome 6 in Nipponbare. Corresponds between the nucleotide residue and the 16,937,072nd nucleotide residue (Table 1). In Nanatsuboshi, mPing is inserted in the polymorphism (4) (SEQ ID NO: 4, FIG. 4), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 64, FIG. 56). The nucleotide sequence of SEQ ID NO: 64 is the nucleotide sequence of nucleotide residues from 16,936,342 to 16,937,718 of chromosome 6 of Nipponbare, and SEQ ID NO: 4 corresponds to the same part. Is an array.

Polymorphism (5): presence or absence of mPing between the 692nd nucleotide residue and the 693rd nucleotide residue of the nucleotide sequence of SEQ ID NO: 65, that is, the 693 to 1122nd nucleotide residues of the nucleotide sequence of SEQ ID NO: 5 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 65 is the nucleotide sequence of nucleotide residues from the 19,790,052 to 19,791,435 nucleotides of Nipponbare chromosome 10, and SEQ ID NO: 5 corresponds to the same part This is the Nanatsuboshi array. The insertion site of mPing in polymorphism (5) is between positions 692 and 693 of SEQ ID NO: 65. When mPing is inserted, the portion of nucleotide residues 693 to 1122 of SEQ ID NO: 5 is mPing It corresponds to the insertion site (underlined part in FIG. 5). The insertion site of mPing in the polymorphism (5) (between the 692nd nucleotide residue and the 693rd nucleotide residue of the nucleotide sequence of SEQ ID NO: 65) is located at the 19th, 790th and 743rd positions of chromosome 10 in Nipponbare. Corresponds to the nucleotide residue and the 19,790,744th nucleotide residue (Table 1). In Nanatsuboshi, mPing is inserted in the polymorphism (5) (SEQ ID NO: 5, FIG. 5), whereas no mPing is inserted in Nipponbare (SEQ ID NO: 65, FIG. 57).

Polymorphism (6): Presence or absence of mPing between the 736th nucleotide residue and the 737th nucleotide residue of the nucleotide sequence of SEQ ID NO: 66, that is, the 737-1166th nucleotide residue of the nucleotide sequence of SEQ ID NO: 6 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 61 is the nucleotide sequence of nucleotide residues 13, 799, 528 to 14,800, 923 of Nipponbare chromosome 4, and SEQ ID NO: 6 corresponds to the same part It is an array of haenuki The insertion site of mPing in the polymorphism (6) is between the 736th and 737th positions of SEQ ID NO: 66. When mPing is inserted, the portion of the nucleotide residues from 737 to 1166 of SEQ ID NO: 6 is This corresponds to the mPing insertion site (underlined portion in FIG. 6). The insertion site of mPing in the polymorphism (6) (between the 736th nucleotide residue and the 737th nucleotide residue of the nucleotide sequence of SEQ ID NO: 66) is located at the 13th, 800th and 263rd positions of chromosome 4 in Nipponbare. Corresponds between the nucleotide residue and the 13,800,264th nucleotide residue (Table 1). In Haenuki, mPing is inserted in the polymorphism (6) (SEQ ID NO: 6, FIG. 6), whereas in Nihonbare, mPing is not inserted (SEQ ID NO: 66, FIG. 58). In the polymorphism (6), mPing is inserted at the same site as that of Haenuki even in the delicate dance and Yukinko dance.

Polymorphism (7): Presence / absence of mPing between the 712th nucleotide residue and the 713th nucleotide residue of the nucleotide sequence of SEQ ID NO: 67, that is, the nucleotide residues 713 to 1142 of the nucleotide sequence of SEQ ID NO: 7 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 67 is the nucleotide sequence of nucleotide residues 28, 393, 728 to 28, 395, 190 of Nipponbare's chromosome 1, and SEQ ID NO: 7 corresponds to the same part The sequence of Kirara 397. The insertion site of mPing in the polymorphism (7) is between the 712th nucleotide residue and the 713th nucleotide residue of SEQ ID NO: 67, and when mPing is inserted, the 713th to 1142th positions of SEQ ID NO: 7 Corresponds to the mPing insertion site (underlined portion in FIG. 7). The insertion site of mPing in the polymorphism (7) (between the 712th nucleotide residue and the 713th nucleotide residue of the nucleotide sequence of SEQ ID NO: 67) is located at the 28th, 394th, and 439th positions of chromosome 1 in Nipponbare. Corresponds between the nucleotide residue and the 28,394,440th nucleotide residue (Table 1). In Kirara 397, mPing is inserted in the polymorphism (7) (SEQ ID NO: 7, FIG. 7), whereas mPing is not inserted in Nipponbare (SEQ ID NO: 67, FIG. 59). In the polymorphism (7), mPing is inserted in the same site as Kirara 397 even in the furkinko and hoshinoyume.

Polymorphism (8): Presence or absence of mPing between the 685th nucleotide residue and the 686th nucleotide residue of the nucleotide sequence of SEQ ID NO: 68, that is, the nucleotide residues 686 to 1115 of the nucleotide sequence of SEQ ID NO: 8 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 68 is the nucleotide sequence of nucleotide residues 6,146,937 to 6,148,431 of Nipponbare chromosome 5, and SEQ ID NO: 8 corresponds to the same part The sequence of Kirara 397. The insertion site of mPing in the polymorphism (8) is between positions 685 and 686 of SEQ ID NO: 68, and when mPing is inserted, the portion of nucleotide residues 686 to 1115 of SEQ ID NO: 8 is mPing It corresponds to the insertion site (underlined part in FIG. 8). The insertion site of mPing in the polymorphism (8) (between the 685th nucleotide residue and the 686th nucleotide residue of the nucleotide sequence of SEQ ID NO: 68) is located at the 6th, 147th and 621th positions of chromosome 5 in Nipponbare. Corresponds between the nucleotide residue and the 6,147,622th nucleotide residue (Table 1). In Kirara 397, mPing is inserted in the polymorphism (8) (SEQ ID NO: 8, FIG. 8), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 68, FIG. 60). In the polymorphism (8), mPing is inserted at the same site as Kirara 397 even in the dream and rag.

Polymorphism (9): presence or absence of mPing between the 738th nucleotide residue and the 739th nucleotide residue of the nucleotide sequence of SEQ ID NO: 69, that is, the 739 to 1168th nucleotide residues of the nucleotide sequence of SEQ ID NO: 9 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 69 is the nucleotide sequence of nucleotide residues 20, 115, 298 to 20, 116, 750 of Nipponbare chromosome 11, and SEQ ID NO: 9 corresponds to the same part It is a true array. The insertion site of mPing in the polymorphism (9) is between the 738th and 739th positions of SEQ ID NO: 69. When mPing is inserted, the portion of nucleotide residues 739 to 1168 of SEQ ID NO: 9 is mPing It corresponds to the insertion site (underlined part in FIG. 9). The insertion site of mPing in the polymorphism (9) (between the 738th nucleotide residue and the 739th nucleotide residue of the nucleotide sequence of SEQ ID NO: 69) is located at the 20th, 116th, 035th positions of chromosome 11 in Nipponbare. Corresponds between the nucleotide residue and the 20,116,036th nucleotide residue (Table 1). In makugura, mPing is inserted in the polymorphism (9) (SEQ ID NO: 9, FIG. 9), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 69, FIG. 61). In the polymorphism (9), mPing is inserted at the same site as that of the bare braids of tatsukomochi and kinu.

Polymorphism (10): Presence / absence of mPing between the 733th and 734th nucleotide residues of the nucleotide sequence of SEQ ID NO: 70, that is, the 734 to 1163rd nucleotide residues of the nucleotide sequence of SEQ ID NO: 10 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 70 is the nucleotide sequence of nucleotide residues 42, 641, 632 to 42, 642, 939 of Nipponbare's chromosome 1, and SEQ ID NO: 10 corresponds to the same part It is an array of delicate splashes. The insertion site of mPing in the polymorphism (10) is between the 733th and 734th positions of SEQ ID NO: 70. When mPing is inserted, the part of the nucleotide residues from 734 to 1163 of SEQ ID NO: 10 is mPing It corresponds to the insertion site (underlined part in FIG. 10). The insertion site of mPing in the polymorphism (10) (between the 733th and 734th nucleotide residues of the nucleotide sequence of SEQ ID NO: 70) is located at the 42nd, 642nd and 364th positions of chromosome 1 in Nipponbare. Corresponds between the nucleotide residue and nucleotides 42,642,365 (Table 1). In Koshibuki, mPing is inserted in the polymorphism (10) (SEQ ID NO: 10, FIG. 10), whereas no mPing is inserted in Nipponbare (SEQ ID NO: 70, FIG. 62). In the polymorphism (10), mPing is inserted at the same site as that of Kobuki even in Yukinko dance.

Polymorphism (11): Presence or absence of mPing between the 713th nucleotide residue and the 714th nucleotide residue of the nucleotide sequence of SEQ ID NO: 71, that is, the nucleotide residues 714 to 1143 of the nucleotide sequence of SEQ ID NO: 11 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 71 is the nucleotide sequence of nucleotide residues 43,062, 212 to 43,063, 699 of Nipponbare's chromosome 1, and SEQ ID NO: 11 corresponds to the same part It is an array of delicate splashes. The insertion site of mPing in the polymorphism (11) is between the 713th and 714th positions of SEQ ID NO: 71. When mPing is inserted, the portion of the nucleotide residues 714 to 1143 of SEQ ID NO: 11 is mPing It corresponds to the insertion site (underlined part in FIG. 11). The insertion site of mPing in the polymorphism (11) (between the 713th nucleotide residue and the 714th nucleotide residue of the nucleotide sequence of SEQ ID NO: 71) is located at the 43rd, 062, 924th position of chromosome 1 in Nipponbare. Corresponds between the nucleotide residue and the 43,062,925th nucleotide residue (Table 1). In Koshibuki, mPing is inserted in the polymorphism (11) (SEQ ID NO: 11, FIG. 11), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 71, FIG. 63). In the polymorphism (11), mPing is inserted at the same site as that of Kobuki even in Yukinko dance.

Polymorphism (12): Presence / absence of mPing between the 768th and 769th nucleotide residues of the nucleotide sequence of SEQ ID NO: 72, that is, the 769 to 1198th nucleotide residues of the nucleotide sequence of SEQ ID NO: 12 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 72 is the nucleotide sequence of nucleotide residues 16336623 to 16338242 of Nipponbare's second chromosome, and SEQ ID NO: 12 is the sequence of the corresponding romance corresponding to the same portion. The insertion site of mPing in the polymorphism (12) is between the 768th and 769th positions of SEQ ID NO: 72. When mPing is inserted, the portion of the nucleotide residues from 769 to 1198 of SEQ ID NO: 12 is mPing It corresponds to the insertion site (underlined part in FIG. 12). The insertion site of mPing in the polymorphism (12) (between the 768th nucleotide residue and the 769th nucleotide residue of the nucleotide sequence of SEQ ID NO: 72) is located at the 16th, 337th, and 390th chromosomes of Nipponbare. Corresponds between the nucleotide residue and the 16,337,391th nucleotide residue (Table 1). In the Tsugaru romance, mPing is inserted in the polymorphism (12) (SEQ ID NO: 12, FIG. 12), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 72, FIG. 64). In the polymorphism (12), mPing is inserted at the same site as the romance in Kinuhikari, Kinusume, Nikkoru, Nasuhikari and Akisakari.

Polymorphism (13): Presence or absence of mPing between the 732nd nucleotide residue and the 733th nucleotide residue of the nucleotide sequence of SEQ ID NO: 73, that is, the 733th to 1162nd nucleotide residues of the nucleotide sequence of SEQ ID NO: 13 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 73 is the nucleotide sequence of nucleotide residues 238848223 to 23881962 of Nipponbare Chromosome 3, and SEQ ID NO: 13 is the sequence of Kinuhikari corresponding to the same portion. The insertion site of mPing in the polymorphism (13) is between the 732rd and 733th positions of SEQ ID NO: 73. When mPing is inserted, the portion of nucleotide residues 733 to 1162 of SEQ ID NO: 13 is mPing. It corresponds to the insertion site (underlined part in FIG. 13). The insertion site of mPing in the polymorphism (13) (between the 732th and 733th nucleotide residues of the nucleotide sequence of SEQ ID NO: 73) is located at the 23rd, 885th and 554th positions of chromosome 3 in Nipponbare. It corresponds to the nucleotide residue and nucleotide residues 23,885 and 555 (Table 1). In Kinuhikari, mPing is inserted in the polymorphism (13) (SEQ ID NO: 13, FIG. 13), whereas in Nihonbare, mPing is not inserted (SEQ ID NO: 73, FIG. 65).

Polymorphism (14): presence or absence of mPing between the 531st nucleotide residue and the 532nd nucleotide residue of the nucleotide sequence of SEQ ID NO: 74, that is, the 532nd to 961th nucleotide residues of the nucleotide sequence of SEQ ID NO: 14 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 74 is the nucleotide sequence of nucleotide residues 328527292 to 32855854 of Nipponbare Chromosome 3, and SEQ ID NO: 14 is the sequence of Asahi's dream corresponding to the same part. The insertion site of mPing in polymorphism (14) is between positions 531 and 532 of SEQ ID NO: 74. When mPing is inserted, the portion of nucleotide residues 532 to 961 of SEQ ID NO: 14 is mPing It corresponds to the insertion site (underlined part in FIG. 14). The insertion site of mPing in the polymorphism (14) (between the 531st nucleotide residue and the 532nd nucleotide residue of the nucleotide sequence of SEQ ID NO: 74) is located at the 32nd, 857th and 822th positions of chromosome 3 in Nipponbare. Corresponds between the nucleotide residue and nucleotides 32,857 and 823 (Table 1). In Asahi's dream, mPing is inserted in the polymorphism (14) (SEQ ID NO: 14, FIG. 14), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 74, FIG. 66).

Polymorphism (15): Presence or absence of mPing between the 722nd nucleotide residue and the 723rd nucleotide residue of the nucleotide sequence of SEQ ID NO: 75, that is, nucleotide residues 723 to 1152 of the nucleotide sequence of SEQ ID NO: 15 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 75 is the nucleotide sequence of nucleotide residues 22,663,944 to 22,665,364 of Nipponbare chromosome 7, and SEQ ID NO: 15 corresponds to the same part It is a dream arrangement. The insertion site of mPing in polymorphism (15) is between positions 722 and 723 of SEQ ID NO: 75. When mPing is inserted, the portion of nucleotide residues 723 to 1152 of SEQ ID NO: 15 is mPing It corresponds to the insertion site (underlined part in FIG. 15). The insertion site of mPing in the polymorphism (15) (between the 722nd nucleotide residue and the 723rd nucleotide residue of the nucleotide sequence of SEQ ID NO: 75) is located at the 22nd, 664th, and 665th chromosomes in Nipponbare. Corresponds between the nucleotide residue and the 22,664,666th nucleotide residue (Table 1). In Yumepirika, mPing is inserted in the polymorphism (15) (SEQ ID NO: 15, FIG. 15), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 75, FIG. 67). In the polymorphism (15), mPing is inserted at the same site as Yumepirika in Humpkinko, Hoshino Yume, Kaze no Komochi, Hakuchomochi and Kitayukimochi.

Polymorphism (16): Presence or absence of mPing between the 753rd nucleotide residue and the 754th nucleotide residue of the base sequence of SEQ ID NO: 76, that is, the 754th to 1183th nucleotide residues of the base sequence of SEQ ID NO: 16 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 76 is the nucleotide sequence of nucleotide residues 15,440,837 to 15,442,335 of the chromosome 9 of Nipponbare, and SEQ ID NO: 16 corresponds to the same part It is an array of Fusakogane. The insertion site of mPing in the polymorphism (16) is between the 753rd and 754th positions of SEQ ID NO: 76. When mPing is inserted, the portion of nucleotide residues 754 to 1183 of SEQ ID NO: 16 is mPing It corresponds to the insertion site (underlined part in FIG. 16). The insertion site of mPing in the polymorphism (16) (between the 753rd nucleotide residue and the 754th nucleotide residue of the nucleotide sequence of SEQ ID NO: 76) is located at the 15th, 441th and 589th positions of chromosome 9 in Nipponbare. Corresponds between the nucleotide residue and the 15,441,590th nucleotide residue (Table 1). In Fusakogane, mPing is inserted in the polymorphism (16) (SEQ ID NO: 16, FIG. 16), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 76, FIG. 68).

Polymorphism (17): presence or absence of mPing between the 727th nucleotide residue and the 728th nucleotide residue of the nucleotide sequence of SEQ ID NO: 77, that is, the nucleotide residues of 728 to 1157th nucleotide sequence of SEQ ID NO: 17 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 77 is the nucleotide sequence of nucleotide residues from 7,690,572 to 7,691,951 of Nipponbare chromosome 3, and SEQ ID NO: 17 corresponds to the same part It is an array of Tsuyahime. The insertion site of mPing in the polymorphism (17) is between the 727th and 728th positions of SEQ ID NO: 77. When mPing is inserted, the portion of nucleotide residues 728 to 1157 of SEQ ID NO: 17 is mPing It corresponds to the insertion site (underlined part in FIG. 17). The insertion site of mPing in the polymorphism (17) (between the 727th nucleotide residue and the 728th nucleotide residue of the nucleotide sequence of SEQ ID NO: 77) is located at the 7,691,298th position of chromosome 3 in Nipponbare. Corresponds between the nucleotide residue and the 7,691,299th nucleotide residue (Table 1). In Tsuyahime, mPing is inserted in the polymorphism (17) (SEQ ID NO: 17, FIG. 17), whereas mPing is not inserted in Nipponbare (SEQ ID NO: 77, FIG. 69).

Polymorphism (18): presence or absence of mPing between the 716th nucleotide residue and the 717th nucleotide residue of the nucleotide sequence of SEQ ID NO: 78, that is, the nucleotide residues 717 to 1146 of the nucleotide sequence of SEQ ID NO: 18 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 78 is the nucleotide sequence of nucleotide residues 31, 165, 097 to 31,166, 522 of Nipponbare's second chromosome, and SEQ ID NO: 18 corresponds to the same part It is an array of Tsuyahime. The insertion site of mPing in the polymorphism (18) is between the 716th and 717th positions of SEQ ID NO: 78. When mPing is inserted, the portion of the nucleotide residues from 717 to 1146 of SEQ ID NO: 18 is mPing It corresponds to the insertion site (underlined part in FIG. 18). The insertion site of mPing in the polymorphism (18) (between the 716th nucleotide residue and the 717th nucleotide residue of the nucleotide sequence of SEQ ID NO: 78) is located at the 31st, 165th and 812nd positions of chromosome 2 in Nipponbare. Corresponds between the nucleotide residue and nucleotides 31,165,813 (Table 1). In Tsuyahime, mPing is inserted in the polymorphism (18) (SEQ ID NO: 18, FIG. 18), whereas in Nihonbare, mPing is not inserted (SEQ ID NO: 78, FIG. 70).

Polymorphism (19): Presence or absence of mPing between the 632nd nucleotide residue and the 633rd nucleotide residue of the nucleotide sequence of SEQ ID NO: 79, that is, the 633th to 1062nd nucleotide residues of the nucleotide sequence of SEQ ID NO: 19 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 79 is the nucleotide sequence of nucleotide residues 15,602,847 to 15,604,289 of the chromosome 3 of Nipponbare, and SEQ ID NO: 19 corresponds to the same part It is an arrangement of Hanaechizen. The insertion site of mPing in the polymorphism (19) is between positions 632 and 633 of SEQ ID NO: 79, and when mPing is inserted, the portion of nucleotide residues 633 to 1062 of SEQ ID NO: 19 is mPing. It corresponds to the insertion site (underlined part in FIG. 19). The insertion site of mPing in the polymorphism (19) (between the 632nd and 633th nucleotide residues of the nucleotide sequence of SEQ ID NO: 79) is located at the 15th, 603rd and 478th positions of chromosome 3 in Nipponbare. Corresponds between the nucleotide residue and the 15,603,479th nucleotide residue (Table 1). In Hanaechizen, mPing is inserted in the polymorphism (19) (SEQ ID NO: 19, FIG. 19), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 79, FIG. 71).

Polymorphism (20): Presence or absence of mPing between the 730th nucleotide residue and the 731st nucleotide residue of the base sequence of SEQ ID NO: 80, that is, the 731st to 1160th nucleotide residues of the base sequence of SEQ ID NO: 20 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 80 is the nucleotide sequence of nucleotide residues 4,491,413 to 4,492,850 of Nipponbare chromosome 3, and SEQ ID NO: 20 corresponds to the same part It is an array of dream horsetails. The insertion site of mPing in the polymorphism (20) is between the 730th and the 731st of SEQ ID NO: 80, and when mPing is inserted, the part of the nucleotide residues from 731 to 1160 of SEQ ID NO: 20 is mPing It corresponds to the insertion site (underlined part in FIG. 20). The insertion site of mPing in the polymorphism (20) (between the 730th nucleotide residue and the 731st nucleotide residue of the nucleotide sequence of SEQ ID NO: 80) is located at the 4th, 492nd and 142nd positions of chromosome 3 in Nipponbare. Corresponds between the nucleotide residue and nucleotides 4,492,143 (Table 1). In Yumekushi, mPing is inserted in the polymorphism (20) (SEQ ID NO: 20, FIG. 20), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 80, FIG. 72). In the polymorphism (20), mPing is inserted in the same site as Yumekushi in Kinuhikari and Nasuhikari.

Polymorphism (21): Presence / absence of mPing between the 686th and 687th nucleotide residues of the nucleotide sequence of SEQ ID NO: 81, that is, the 687th to 1116th nucleotide residues of the nucleotide sequence of SEQ ID NO: 21 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 81 is the nucleotide sequence of nucleotide residues 2,352,935 to 2,354,429 of chromosome 6 of Nipponbare, and SEQ ID NO: 21 corresponds to the same part It is an array of Fusotome. The insertion site of mPing in the polymorphism (21) is between the 686th and 687th positions of SEQ ID NO: 81. When mPing is inserted, the portion of nucleotide residues 687 to 1116 of SEQ ID NO: 21 is mPing. It corresponds to the insertion site (underlined part in FIG. 21). The insertion site of mPing in the polymorphism (21) (between the 686th nucleotide residue and the 687th nucleotide residue of the nucleotide sequence of SEQ ID NO: 81) is located at the 2nd, 353rd and 620th positions of chromosome 6 in Nipponbare. Corresponds between nucleotide residues and nucleotides 2,353,621 (Table 1). In Fusootome, mPing is inserted in the polymorphism (21) (SEQ ID NO: 21, FIG. 21), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 81, FIG. 73). In the polymorphism (21), mPing is inserted at the same site as Fusotome in Hanaechizen and Hashishima.

Polymorphism (22): Presence / absence of mPing between the 727th nucleotide residue and the 728th nucleotide residue of the nucleotide sequence of SEQ ID NO: 82, that is, the 728-1157th nucleotide residues of the nucleotide sequence of SEQ ID NO: 22 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 82 is the nucleotide sequence of nucleotide residues 38, 571, 459 to 38, 572, 942 of Nipponbare's chromosome 1, and SEQ ID NO: 22 corresponds to the same part This is an array. The insertion site of mPing in the polymorphism (22) is between the 727th and 728th positions of SEQ ID NO: 82. When mPing is inserted, the portion of nucleotide residues 728 to 1157 of SEQ ID NO: 22 is mPing This corresponds to the insertion site (underlined portion in FIG. 22). The insertion site of mPing in the polymorphism (22) (between the 727th and 728th nucleotide residues of the nucleotide sequence of SEQ ID NO: 82) is located at the 38th, 572nd and 185th positions of chromosome 1 in Nipponbare. Corresponds between the nucleotide residue and the 38,572,186th nucleotide residue (Table 1). In Fukinko, mPing is inserted in the polymorphism (22) (SEQ ID NO: 22, FIG. 22), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 82, FIG. 74).

Polymorphism (23): Presence / absence of mPing between the 803th nucleotide residue and the 804th nucleotide residue of the nucleotide sequence of SEQ ID NO: 83, that is, the 804th to 1233th nucleotide residues of the nucleotide sequence of SEQ ID NO: 23 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 83 is the nucleotide sequence of nucleotide residues 3, 211, 686 to 3,213, 192 of Nipponbare chromosome 9, and SEQ ID NO: 23 corresponds to the same part This is an array. The insertion site of mPing in the polymorphism (23) is between the 803th and 804th of SEQ ID NO: 83, and when mPing is inserted, the portion of the nucleotide residues 804 to 1233 of SEQ ID NO: 23 is mPing. It corresponds to the insertion site (underlined part in FIG. 23). The insertion site of mPing in the polymorphism (23) (between the 803th nucleotide residue and the 804th nucleotide residue of the nucleotide sequence of SEQ ID NO: 83) is located at the 3,212,488th position of chromosome 9 in Nipponbare. Corresponds between the nucleotide residue and the 3,212,489th nucleotide residue (Table 1). In Fukinko, mPing is inserted in the polymorphism (23) (SEQ ID NO: 23, FIG. 23), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 83, FIG. 75).

Polymorphism (24): presence or absence of mPing between the 694th nucleotide residue and the 695th nucleotide residue of the nucleotide sequence of SEQ ID NO: 84, that is, the 695th to 1124th nucleotide residues of the nucleotide sequence of SEQ ID NO: 24 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 84 is the nucleotide sequence of nucleotide residues 2264289 to 2227856 of Nipponbare's chromosome 4, and SEQ ID NO: 24 is the sequence of the dream corresponding to the same part. The insertion site of mPing in the polymorphism (24) is between positions 694 and 695 of SEQ ID NO: 84. When mPing is inserted, the portion of nucleotide residues 695 to 1124 of SEQ ID NO: 24 is mPing. It corresponds to the insertion site (underlined part in FIG. 24). The insertion site of mPing in the polymorphism (24) (between the 694th nucleotide residue and the 695th nucleotide residue of the nucleotide sequence of SEQ ID NO: 84) is located at the 2nd, 227th and 18th positions of chromosome 4 in Nipponbare. Corresponds between the nucleotide residue and the 2,227,183rd nucleotide residue (Table 1). In Hoshino Yume, mPing is inserted in the polymorphism (24) (SEQ ID NO: 24, FIG. 24), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 84, FIG. 76). In polymorphism (24), mPing is inserted at the same site as Hoshino Yume in Nanatsubo, Kirara 397, Yumepirika, and Orozuki.

Polymorphism (25): Presence / absence of mPing between the 728th and 729th nucleotide residues of the nucleotide sequence of SEQ ID NO: 85, that is, the 729 to 1158th nucleotide residues of the nucleotide sequence of SEQ ID NO: 25 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 85 is the nucleotide sequence of nucleotide residues 170528 to 172067 of Nipponbare's chromosome 5, and SEQ ID NO: 25 is a cumbersome sequence corresponding to the same part. The insertion site of mPing in the polymorphism (25) is between the 728th and 729th positions of SEQ ID NO: 85. When mPing is inserted, the portion of the nucleotide residues 729 to 1158 of SEQ ID NO: 25 is mPing It corresponds to the insertion site (underlined part in FIG. 25). The insertion site of mPing in the polymorphism (25) (between the 728th and 729th nucleotide residues of the nucleotide sequence of SEQ ID NO: 85) is the residue of the 171st and 255th nucleotides of chromosome 5 in Nipponbare. Corresponds between the group and nucleotide positions 171 and 256 (Table 1). In Menkoina, mPing is inserted in the polymorphism (25) (SEQ ID NO: 25, FIG. 25), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 85, FIG. 77). In the polymorphism (25), mPing is inserted at the same site as that of noodles, even if it is straight, Sasanishiki, Tatsukomochi or Kinu no Hada.

Polymorphism (26): Presence / absence of mPing between the 656th and 657th nucleotide residues of the nucleotide sequence of SEQ ID NO: 86, that is, the 657th to 1086th nucleotide residues of the nucleotide sequence of SEQ ID NO: 26 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 86 is the nucleotide sequence of nucleotide residues 19,376,786 to 19,378,114 of Nipponbare chromosome 12, and SEQ ID NO: 26 corresponds to the same part It is an arrangement of Kinusume. The insertion site of mPing in the polymorphism (26) is between the 656th and 657th positions of SEQ ID NO: 86. When mPing is inserted, the nucleotide residues of the 657th to 1086th nucleotide positions of SEQ ID NO: 26 are This corresponds to the insertion site (underlined portion in FIG. 26). The insertion site of mPing in the polymorphism (26) (between the 656th nucleotide residue and the 657th nucleotide residue of the nucleotide sequence of SEQ ID NO: 86) is located at the 19th, 377th and 441th positions of chromosome 12 in Nipponbare. Corresponds between the nucleotide residue and the 19,377,442nd nucleotide residue (Table 1). In Kinusume, mPing is inserted in the polymorphism (26) (SEQ ID NO: 26, FIG. 16), whereas mPing is not inserted in Nipponbare (SEQ ID NO: 86, FIG. 78).

Polymorphism (27): Presence or absence of mPing between nucleotide residues 703 and 704 of the nucleotide sequence of SEQ ID NO: 87, that is, nucleotide residues 704 to 1133 of the nucleotide sequence of SEQ ID NO: 27 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 87 is the nucleotide sequence of nucleotide residues from the 17,090,777th position to the 17,092,231st nucleotide of Nipponbare chromosome 9, and SEQ ID NO: 27 corresponds to the same part It is an array of Kaori Aichi. The insertion site of mPing in the polymorphism (27) is between the 703th and 704th positions of SEQ ID NO: 87. When mPing is inserted, the portion of nucleotide residues 704 to 1133 of SEQ ID NO: 27 is mPing. It corresponds to the insertion site (underlined part in FIG. 27). The insertion site of mPing in the polymorphism (27) (between the 703th and 704th nucleotide residues of the nucleotide sequence of SEQ ID NO: 87) is located at the 17th, 091, 479th position of chromosome 9 in Nipponbare. Corresponds between the nucleotide residue and nucleotide residues 17,091, 480 (Table 1). In Aichi no Kaori, mPing is inserted in the polymorphism (27) (SEQ ID NO: 27, FIG. 27), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 87, FIG. 79).

Polymorphism (28): presence or absence of mPing between nucleotide residues 704 and 705 of the nucleotide sequence of SEQ ID NO: 88, that is, nucleotide residues 705 to 1134 of the nucleotide sequence of SEQ ID NO: 28 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 88 is the nucleotide sequence of nucleotide residues 25, 446, 945 to 25, 448, 351 of Nipponbare chromosome 2, and SEQ ID NO: 28 corresponds to the same part It is a rough arrangement. The insertion site of mPing in the polymorphism (28) is between positions 704 and 705 of SEQ ID NO: 88. When mPing is inserted, the nucleotide residues at positions 705 to 1134 of SEQ ID NO: 28 are mPing. It corresponds to the insertion site (underlined part in FIG. 28). The insertion site of mPing in the polymorphism (28) (between the 704th and 705th nucleotide residues of the nucleotide sequence of SEQ ID NO: 88) is located at the 25th, 447th and 648th positions of chromosome 2 in Nipponbare. Corresponds between nucleotide residues and nucleotide residues 25,447,649 (Table 1). In the rough outline, mPing is inserted in the polymorphism (28) (SEQ ID NO: 28, FIG. 28), whereas mPing is not inserted in Nipponbare (SEQ ID NO: 88, FIG. 80).

Polymorphism (29): Presence or absence of mPing between the 696th and 697th nucleotide residues of the nucleotide sequence of SEQ ID NO: 89, that is, the 697 to 1126th nucleotide residues of the nucleotide sequence of SEQ ID NO: 29 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 89 is the nucleotide sequence of nucleotide residues 1,136,773 from 1,138,106 of Nipponbare chromosome 8, and SEQ ID NO: 29 corresponds to the same part It is a rough arrangement. The insertion site of mPing in the polymorphism (29) is between the 696th and 697th positions of SEQ ID NO: 89, and when mPing is inserted, the portion of nucleotide residues 697 to 1126 of SEQ ID NO: 29 is mPing It corresponds to the insertion site (underlined part in FIG. 29). The insertion site of mPing in the polymorphism (29) (between the 696th and 697th nucleotide residues in the nucleotide sequence of SEQ ID NO: 89) is located at the 1st, 137th and 468th positions of chromosome 8 in Nipponbare. Corresponds between nucleotide residues and nucleotide residues 1,137,469 (Table 1). In the rough outline, mPing is inserted in the polymorphism (29) (SEQ ID NO: 29, FIG. 29), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 89, FIG. 81).

Polymorphism (30): Presence / absence of mPing between the 800th nucleotide residue and the 801st nucleotide residue of the nucleotide sequence of SEQ ID NO: 90, that is, the 801-1230th nucleotide residues of the nucleotide sequence of SEQ ID NO: 30 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 90 is the nucleotide sequence of nucleotide residues 847948 to 847426 of Nipponbare's chromosome 5, and SEQ ID NO: 30 is the sequence of Yume Mizuho corresponding to the same part. The insertion site of mPing in the polymorphism (30) is between the 800th and 801st positions of SEQ ID NO: 90. When mPing is inserted, the portion of the nucleotide residues from 801 to 1230 of SEQ ID NO: 30 is mPing It corresponds to the insertion site (underlined part in FIG. 30). The insertion site of mPing in the polymorphism (30) (between the 800th nucleotide residue and the 801st nucleotide residue of the nucleotide sequence of SEQ ID NO: 90) is the residue of the 848th and 747th nucleotides of chromosome 5 in Nipponbare. Corresponds between the group and the 848,748th nucleotide residue (Table 1). In Yume Mizuho, mPing is inserted in the polymorphism (30) (SEQ ID NO: 30, FIG. 30), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 90, FIG. 82).

Polymorphism (31): Presence or absence of mPing between the 747th nucleotide residue and the 748th nucleotide residue of the nucleotide sequence of SEQ ID NO: 91, that is, the nucleotide residues 748 to 1177 of the nucleotide sequence of SEQ ID NO: 31 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 91 is the nucleotide sequence of nucleotide residues 18, 779, 621 to 18,780, 943 of Nipponbare chromosome 11, and SEQ ID NO: 31 corresponds to the same part Sasanishiki sequence. The insertion site of mPing in the polymorphism (31) is between positions 747 and 748 of SEQ ID NO: 91. When mPing is inserted, the portion of nucleotide residues 748 to 1177 of SEQ ID NO: 31 is mPing. It corresponds to the insertion site (underlined part in FIG. 31). The insertion site of mPing in the polymorphism (31) (between the 747th nucleotide residue and the 748th nucleotide residue of the nucleotide sequence of SEQ ID NO: 91) is located at the 18th, 780th and 367th positions of chromosome 11 in Nipponbare. Corresponds between nucleotide residues and nucleotides 18,780,368 (Table 1). In Sasanishiki, mPing is inserted in the polymorphism (31) (SEQ ID NO: 31, FIG. 31), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 91, FIG. 83).

Polymorphism (32): the presence or absence of mPing between the 722nd nucleotide residue and the 723rd nucleotide residue of the nucleotide sequence of SEQ ID NO: 92, that is, the nucleotide residues 723 to 1152 of the nucleotide sequence of SEQ ID NO: 32 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 92 is the nucleotide sequence of nucleotide residues 276, 238 to 277, 676 of Nipponbare chromosome 8, and SEQ ID NO: 32 is the milky queen sequence corresponding to the same part It is. The insertion site of mPing in the polymorphism (32) is between positions 722 and 723 of SEQ ID NO: 92. When mPing is inserted, the portion of nucleotide residues 723 to 1152 of SEQ ID NO: 32 is mPing. It corresponds to the insertion site (underlined part in FIG. 32). The insertion site of mPing in the polymorphism (32) (between the 722nd and 723rd nucleotide residues of the nucleotide sequence of SEQ ID NO: 92) is the residue of the 276th and 959th nucleotides of chromosome 8 in Nipponbare. Corresponds between the group and the 276th, 960th nucleotide residue (Table 1). In Milky Queen, mPing is inserted in the polymorphism (32) (SEQ ID NO: 32, FIG. 32), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 92, FIG. 84).

Polymorphism (33): Presence / absence of mPing between the 650th and 651th nucleotide residues of the nucleotide sequence of SEQ ID NO: 93, that is, the 651 to 1080th nucleotide residues of the nucleotide sequence of SEQ ID NO: 33 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 93 is the nucleotide sequence of nucleotide residues 30, 123, 787 to 30, 125, 101 of Nipponbare chromosome 3, and SEQ ID NO: 33 corresponds to the same part It is an array of cypress. The insertion site of mPing in the polymorphism (33) is between the 650th and 651th positions of SEQ ID NO: 93. When mPing is inserted, the portion of nucleotide residues 651 to 1080 of SEQ ID NO: 33 is mPing It corresponds to the insertion site (underlined part in FIG. 33). The insertion site of mPing in the polymorphism (33) (between the 650th and 651th nucleotide residues of the nucleotide sequence of SEQ ID NO: 93) is located at the 30th, 124th and 436th positions of chromosome 3 in Nipponbare. Corresponds between the nucleotide residue and the 30,124,437th nucleotide residue (Table 1). In chickweed, mPing is inserted in the polymorphism (33) (SEQ ID NO: 33, FIG. 33), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 93, FIG. 85).

Polymorphism (34): Presence or absence of mPing between the 733th nucleotide residue and the 734th nucleotide residue of the nucleotide sequence of SEQ ID NO: 94, that is, the nucleotide residues 734 to 1163 of the nucleotide sequence of SEQ ID NO: 34 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 94 is the nucleotide sequence of nucleotide residues 30, 572, 355 to 30,573, 800 of Nipponbare's chromosome 2, and SEQ ID NO: 34 corresponds to the same part It is an array of cypress. The insertion site of mPing in the polymorphism (34) is between the 733th and 734th positions of SEQ ID NO: 94. When mPing is inserted, the part of the nucleotide residues from 734 to 1163 of SEQ ID NO: 34 is mPing. It corresponds to the insertion site (underlined part in FIG. 34). The insertion site of mPing in the polymorphism (34) (between the 733th and 734th nucleotide residues of the nucleotide sequence of SEQ ID NO: 94) is located at the 30th, 573rd and 087th positions of chromosome 2 in Nipponbare. Corresponds between the nucleotide residue and nucleotides 30,573,088 (Table 1). In chickweed, mPing is inserted in the polymorphism (34) (SEQ ID NO: 34, FIG. 34), whereas in Japan Hare, mPing is not inserted (SEQ ID NO: 94, FIG. 86).

Polymorphism (35): Presence or absence of mPing between the 578th and 579th nucleotide residues of the nucleotide sequence of SEQ ID NO: 95, that is, the 579 to 1008th nucleotide residues of the nucleotide sequence of SEQ ID NO: 35 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 95 is the nucleotide sequence of nucleotide residues 27,459,779 to 27,461,032 of Nipponbare chromosome 8, and SEQ ID NO: 35 corresponds to the same part It is a sequence of Hymenomochi. The insertion site of mPing in the polymorphism (35) is between the 578th and 579th positions of SEQ ID NO: 95. When mPing is inserted, the portion of the nucleotide residues 579 to 1008 of SEQ ID NO: 35 is mPing. It corresponds to the insertion site (underlined part in FIG. 35). The insertion site of mPing in the polymorphism (35) (between the 578th and 579th nucleotide residues of the nucleotide sequence of SEQ ID NO: 95) is located at the 27th, 460th and 356th positions of chromosome 8 in Nipponbare. Corresponds between the nucleotide residue and the 27,460,357th nucleotide residue (FIG. 35, Table 1). In Hymenomochi, mPing is inserted in the polymorphism (35) (SEQ ID NO: 35, FIG. 35), whereas in Nihonbare, mPing is not inserted (SEQ ID NO: 95, FIG. 87). In polymorphism (35), mPing is inserted at the same site as Hymenomochi even in Nikkomaru, Manamusume, Chiyonoshiki and Watabushi.

Polymorphism (36): Presence / absence of mPing between the 879th nucleotide residue and the 880th nucleotide residue of the nucleotide sequence of SEQ ID NO: 96, that is, the 880-1309th nucleotide residues of the nucleotide sequence of SEQ ID NO: 36 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 96 is the nucleotide sequence of nucleotide residues 20, 708, 301 to 20, 709, 792 of Nipponbare chromosome 6, and SEQ ID NO: 36 corresponds to the same part It is an array of koganechi. The insertion site of mPing in the polymorphism (36) is between the 879th and 880th positions of SEQ ID NO: 96, and when mPing is inserted, the part of the nucleotide residues from 880 to 1309 of SEQ ID NO: 36 is mPing. It corresponds to the insertion site (underlined part in FIG. 36). The insertion site of mPing in the polymorphism (36) (between the 879th nucleotide residue and the 880th nucleotide residue of the nucleotide sequence of SEQ ID NO: 96) is located at the 20th, 709th, and 179th positions of chromosome 6 in Nipponbare. Corresponds between the nucleotide residue and the 20,709,180th nucleotide residue (Table 1). In kokomochi, mPing is inserted in the polymorphism (36) (SEQ ID NO: 36, FIG. 36), whereas in Japan Hare, mPing is not inserted (SEQ ID NO: 96, FIG. 88).

Polymorphism (37): Presence or absence of mPing between the 718th nucleotide residue and the 719th nucleotide residue of the nucleotide sequence of SEQ ID NO: 97, that is, the nucleotide residues 732 to 1161 of the nucleotide sequence of SEQ ID NO: 37 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 97 is the nucleotide sequence of nucleotide residues 3,543,063 to 3,544,439 of Nipponbare chromosome 8, and SEQ ID NO: 37 corresponds to the same part It is an array of koganechi. The insertion site of mPing in the polymorphism (37) is between the 718th and 719th positions of SEQ ID NO: 97, and when mPing is inserted, the portion of nucleotide residues 732 to 1161 of SEQ ID NO: 37 is mPing. It corresponds to the insertion site (underlined part in FIG. 37). The insertion site of mPing in the polymorphism (37) (between the 718th nucleotide residue and the 719th nucleotide residue of the nucleotide sequence of SEQ ID NO: 97) is located at the 3rd, 543th and 780th positions of chromosome 8 in Nipponbare. Corresponds between the nucleotide residue and nucleotides 3,543,781 (Table 1). In Kokomochi, mPing is inserted in the polymorphism (37) (SEQ ID NO: 37, FIG. 37), whereas in Japan Hare, mPing is not inserted (SEQ ID NO: 97, FIG. 89).

Polymorphism (38): Presence / absence of mPing between the 756th nucleotide residue and the 757th nucleotide residue of the nucleotide sequence of SEQ ID NO: 98, that is, the nucleotide residues 757 to 1186 of the nucleotide sequence of SEQ ID NO: 38 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 98 is the nucleotide sequence of nucleotide residues 31, 580, 655 to 31,581, 994 of the third chromosome of Nipponbare, and SEQ ID NO: 38 corresponds to the same part It is an array of children of wind. The insertion site of mPing in the polymorphism (38) is between the 756th and 757th positions of SEQ ID NO: 98, and when mPing is inserted, the portion of nucleotide residues from 757 to 1186 of SEQ ID NO: 38 is mPing. It corresponds to the insertion site (underlined part in FIG. 38). The insertion site of mPing in the polymorphism (38) (between the 756th nucleotide residue and the 757th nucleotide residue of the nucleotide sequence of SEQ ID NO: 98) is located at the 31st, 581, 410th position of chromosome 3 in Nipponbare. Corresponds between the nucleotide residue and the 31,581,411st nucleotide residue (Table 1). In the wind child, mPing is inserted in the polymorphism (38) (SEQ ID NO: 38, FIG. 38), whereas in Japan Hare, mPing is not inserted (SEQ ID NO: 98, FIG. 90). In the polymorphism (38), mPing is inserted at the same site as the wind puppet, whether it is snug or squeezed.

Polymorphism (39): presence or absence of mPing between the 689th nucleotide residue and the 690th nucleotide residue of the nucleotide sequence of SEQ ID NO: 99, that is, the 690th to 1119th nucleotide residues of the nucleotide sequence of SEQ ID NO: 39 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 99 is the nucleotide sequence of nucleotide residues 24,906,269 to 24,907,644 of Nipponbare chromosome 6, and SEQ ID NO: 39 corresponds to the same part It is an arrangement of cotton buds. The insertion site of mPing in the polymorphism (39) is between positions 689 and 690 of SEQ ID NO: 99, and when mPing is inserted, it is a part of nucleotide residues 690 to 1119 of SEQ ID NO: 39 (Underlined portion in FIG. 39). The insertion site of mPing in the polymorphism (39) (between the 689th nucleotide residue and the 690th nucleotide residue of the nucleotide sequence of SEQ ID NO: 99) is located at the 24th, 906th and 957th positions of chromosome 6 in Nipponbare. Corresponds between nucleotide residues and nucleotide residues 24,906,958 (Table 1). In Wataboshi, mPing is inserted in the polymorphism (39) (SEQ ID NO: 39, FIG. 39), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 99, FIG. 91).

Polymorphism (40): Presence or absence of mPing between the 733th nucleotide residue and the 734th nucleotide residue of the base sequence of SEQ ID NO: 100, that is, the 734 to 1163rd nucleotide residues of the base sequence of SEQ ID NO: 40 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 100 is the nucleotide sequence of nucleotide residues 4,526,756 to 4,528,294 of Nipponbare chromosome 11, and SEQ ID NO: 40 corresponds to the same part It is an arrangement of cotton buds. The insertion site of mPing in the polymorphism (40) is between the 733th and 734th positions of SEQ ID NO: 100. When mPing is inserted, the part of the nucleotide residues from 734 to 1163 of SEQ ID NO: 40 is mPing. It corresponds to the insertion site (underlined part in FIG. 40). The insertion site of mPing in the polymorphism (40) (between the 733th and 734th nucleotide residues of the nucleotide sequence of SEQ ID NO: 100) is located at the 4th, 527th and 488th positions of chromosome 11 in Nipponbare. Corresponds between the nucleotide residue and the 4,527,489th nucleotide residue (FIG. 40, Table 1). In Wataboshi, mPing is inserted in the polymorphism (40) (SEQ ID NO: 40, FIG. 40), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 100, FIG. 92).

Polymorphism (41): presence or absence of mPing between the 729th nucleotide residue and the 730th nucleotide residue of the base sequence of SEQ ID NO: 101, that is, the 730-1159th nucleotide residues of the base sequence of SEQ ID NO: 41 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 101 is the nucleotide sequence of nucleotide residues 15, 394, 502 to 15,395, 921 of Nipponbare chromosome 2, and SEQ ID NO: 41 corresponds to the same part Is an array of rice cakes. The insertion site of mPing in the polymorphism (41) is between the 729th and 730th positions of SEQ ID NO: 101. When mPing is inserted, the portion of nucleotide residues 730 to 1159 of SEQ ID NO: 41 is mPing It corresponds to the insertion site (underlined part in FIG. 41). The insertion site of mPing in the polymorphism (41) (between the 729th nucleotide residue and the 730th nucleotide residue of the nucleotide sequence of SEQ ID NO: 101) is located at the 15th, 395th and 230th positions of chromosome 2 in Nipponbare. Corresponds between the nucleotide residue and the 15,395,231st nucleotide residue (Table 1). In Hakuchomochi, mPing is inserted in polymorphism (41) (SEQ ID NO: 41, FIG. 41), whereas mPing is not inserted in Nipponbare (SEQ ID NO: 101, FIG. 93).

Polymorphism (42): presence or absence of mPing between the 705th nucleotide residue and the 706th nucleotide residue of the nucleotide sequence of SEQ ID NO: 102, that is, the nucleotide residues 706 to 1135 of the nucleotide sequence of SEQ ID NO: 42 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 102 is the nucleotide sequence of nucleotide residues 28,396,556 to 28,397,996 of Nipponbare chromosome 3, and SEQ ID NO: 42 corresponds to the same part Is an array of rice cakes. The insertion site of mPing in the polymorphism (42) is between positions 705 and 706 of SEQ ID NO: 102, and when mPing is inserted, the nucleotide residues at positions 706 to 1135 of SEQ ID NO: 42 are mPing. It corresponds to the insertion site (underlined part in FIG. 42). The insertion site of mPing in the polymorphism (42) (between the 705th nucleotide residue and the 706th nucleotide residue of the nucleotide sequence of SEQ ID NO: 102) is located at the 28th, 397th and 260th positions of chromosome 3 in Nipponbare. Corresponds between the nucleotide residue and the 28,397,261st nucleotide residue (Table 1). In Hakuchomochi, mPing is inserted in the polymorphism (42) (SEQ ID NO: 42, FIG. 42), whereas no mPing is inserted in Nipponbare (SEQ ID NO: 102, FIG. 94).

Polymorphism (43): Presence / absence of mPing between the 603rd and 604th nucleotide residues of the nucleotide sequence of SEQ ID NO: 103, that is, the 604th to 1033rd nucleotide residues of the nucleotide sequence of SEQ ID NO: 43 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 103 is the nucleotide sequence of nucleotide residues 5,594,136 to 5,595,486 of Nipponbare chromosome 5, and SEQ ID NO: 43 corresponds to the same part It is an array of Tatsukomochi. The insertion site of mPing in the polymorphism (43) is between the 603rd and 604th positions of SEQ ID NO: 103. When mPing is inserted, the portion of the nucleotide residues 604 to 1033 of SEQ ID NO: 43 is mPing. It corresponds to the insertion site (underlined part in FIG. 43). The insertion site of mPing in the polymorphism (43) (between the 603rd and 604th nucleotide residues of the nucleotide sequence of SEQ ID NO: 103) is located at the 5th, 594th and 738th positions of chromosome 5 in Nipponbare. Corresponds between the nucleotide residue and nucleotides 5,594, 739 (Table 1). In Tatsukomochi, mPing is inserted in the polymorphism (43) (SEQ ID NO: 43, FIG. 43), whereas in Nipponbare, mPing is not inserted. In the polymorphism (43), mPing is inserted at the same site as Tatsukomochi even though it is a kinu-no-nabe (SEQ ID NO: 103, FIG. 95).

Polymorphism (44): Presence / absence of mPing between the 727th nucleotide residue and the 728th nucleotide residue of the nucleotide sequence of SEQ ID NO: 104, that is, the nucleotide residues 728 to 1157 of the nucleotide sequence of SEQ ID NO: 44 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 104 is the nucleotide sequence of nucleotide residues from the 17,034,567th to the 17,036th, 109th nucleotides of Nipponbare chromosome 10, and SEQ ID NO: 44 corresponds to the same part It is the arrangement of Kitayukimochi. The insertion site of mPing in the polymorphism (44) is between the 727th and 728th positions of SEQ ID NO: 104. When mPing is inserted, the portion of the nucleotide residues from 728 to 1157 of SEQ ID NO: 44 is mPing. It corresponds to the insertion site (underlined part in FIG. 44). The insertion site of mPing in the polymorphism (44) (between the 727th nucleotide residue and the 728th nucleotide residue of the nucleotide sequence of SEQ ID NO: 104) is located at the 17th, 035th and 293rd positions of chromosome 10 in Nipponbare. Corresponds between the nucleotide residue and the 17,035,294th nucleotide residue (Table 1). In Kitayukimochi, mPing is inserted in the polymorphism (44) (SEQ ID NO: 44, FIG. 44), whereas in Japan Hare, mPing is not inserted (SEQ ID NO: 104, FIG. 96).

Polymorphism (45): Presence or absence of mPing between the 734th and 735th nucleotide residues of the nucleotide sequence of SEQ ID NO: 105, that is, the 735th to 1164th nucleotide residues of the nucleotide sequence of SEQ ID NO: 45 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 105 is the nucleotide sequence of nucleotide residues 26, 368, and 463 to 26, 369, and 916 of Nipponbare chromosome 5, and SEQ ID NO: 45 corresponds to the same part Kinu is an array of bares. The insertion site of mPing in the polymorphism (45) is between the 734th and 735th positions of SEQ ID NO: 105. When mPing is inserted, the portion of the nucleotide residues from 735 to 1164 of SEQ ID NO: 45 is mPing. It corresponds to the insertion site (underlined part in FIG. 45). The insertion site of mPing in the polymorphism (45) (between the 734th and 735th nucleotide residues of the nucleotide sequence of SEQ ID NO: 105) is located at the 26th, 369th, and 196th positions of chromosome 5 in Nipponbare. Corresponds between the nucleotide residue and the 26,369,197th nucleotide residue (Table 1). In Kinu Hata, mPing is inserted in the polymorphism (45) (SEQ ID NO: 45, FIG. 45), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 105, FIG. 97).

Polymorphism (46): Presence or absence of mPing between the 653rd nucleotide residue and the 654th nucleotide residue of the base sequence of SEQ ID NO: 106, that is, the 654th to 1083th nucleotide residues of the base sequence of SEQ ID NO: 46 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 106 is the nucleotide sequence of nucleotide residues 19,817,499 to 19,818,814 of Nipponbare chromosome 4, and SEQ ID NO: 46 corresponds to the same part It is an array of Nishiki Yamada. The insertion site of mPing in the polymorphism (46) is between the 653rd and 654th positions of SEQ ID NO: 106, and when mPing is inserted, the portion of nucleotide residues 654 to 1083 of SEQ ID NO: 46 is mPing. It corresponds to the insertion site (underlined part in FIG. 46). The insertion site of mPing in the polymorphism (46) (between the 653rd nucleotide residue and the 654th nucleotide residue of the nucleotide sequence of SEQ ID NO: 106) is located at the 19th, 818th and 151st positions of chromosome 4 in Nipponbare. Corresponds between the nucleotide residue and the 19,818,152th nucleotide residue (Table 1). In Yamada Nishiki, mPing is inserted in the polymorphism (46) (SEQ ID NO: 46, FIG. 46), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 106, FIG. 98).

Polymorphism (47): Presence or absence of mPing between the 726th and 727th nucleotide residues of the nucleotide sequence of SEQ ID NO: 107, that is, the 727 to 1156th nucleotide residues of the nucleotide sequence of SEQ ID NO: 47 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 107 is the nucleotide sequence of nucleotide residues 15, 177, 100 to 15,178, 536 of Nipponbare chromosome 8, and SEQ ID NO: 47 corresponds to the same part It is an array of Nishiki Yamada. The insertion site of mPing in the polymorphism (47) is between the 726th and 727th positions of SEQ ID NO: 107. When mPing is inserted, the portion of the nucleotide residues from 727 to 1156 of SEQ ID NO: 47 is mPing. It corresponds to the insertion site (underlined part in FIG. 47). The insertion site of mPing in the polymorphism (47) (between the 726th and 727th nucleotide residues of the nucleotide sequence of SEQ ID NO: 107) is located at the 15th, 177th and 825th positions of chromosome 8 in Nipponbare. Corresponds between the nucleotide residue and the 15,177,826th nucleotide residue (Table 1). In Yamada Nishiki, mPing is inserted in the polymorphism (47) (SEQ ID NO: 47, FIG. 47), whereas in Nipponbare, mPing is not inserted (SEQ ID NO: 107, FIG. 99).

Polymorphism (48): Presence / absence of mPing between the 626th and 627th nucleotide residues of the nucleotide sequence of SEQ ID NO: 108, that is, the 627th to 1056th nucleotide residues of the nucleotide sequence of SEQ ID NO: 48 The presence or absence of mPing The nucleotide sequence of SEQ ID NO: 108 is the nucleotide sequence of nucleotide residues 1,245,566 to 1,246,826 of Nipponbare chromosome 3, and SEQ ID NO: 48 corresponds to the same part It is an array of 500 million stones. The insertion site of mPing in the polymorphism (48) is between the 626th and 627th positions of SEQ ID NO: 108. When mPing is inserted, the portion of the nucleotide residues 627 to 1056 of SEQ ID NO: 48 is mPing. It corresponds to the insertion site (underlined part in FIG. 48). The insertion site of mPing in the polymorphism (48) (between the 626th nucleotide residue and the 627th nucleotide residue of the nucleotide sequence of SEQ ID NO: 108) is located at the 1st, 246th and 191st positions of chromosome 3 in Nipponbare. Corresponds between nucleotide residues and nucleotides 1,246,192 (Table 1). In Hyakumangoku, mPing was inserted in polymorphism (48) (SEQ ID NO: 48, FIG. 48), whereas mPing was not inserted in Nipponbare (SEQ ID NO: 108, FIG. 100).

  The insertion positions on the Nipponbare genome (obtained from (IRGSP-1.0)) of the mPing insertion site in polymorphisms (1) to (48) are as shown in Table 1 below.

  The “insertion position” in Table 1 is between the last “T” of TAA or TTA and the base immediately after it.

  As a method for confirming the corresponding position in the rice genome of each nucleotide sequence of SEQ ID NOs: 1 to 48, a method of referring to a publicly available genome database or the like, a method of performing a homologous search with a genome sequence based on each polymorphic nucleotide sequence Etc. are exemplified. Each base sequence of SEQ ID NOs: 1 to 48 may contain a variation unique to rice varieties. For example, the variation with Nipponbare is as shaded in some of FIGS. It is not limited to the polymorphisms shown in these figures, and can occur in all of the polymorphisms (1) to (48). These nucleotide sequences are several (for example, within 50, within 40, within 30, within 20 and within 10, preferably within 5 and more preferably within 4; More preferably, it is 3 or less, even more preferably 2 or less, and most preferably 1), which may be a base sequence containing a base modification (eg, substitution, deletion, insertion, addition). Such an equivalent base sequence can be determined by referring to a database in which gene information of rice varieties is registered or by decoding a base sequence around the insertion region of the target mPing in rice varieties. .

The rice variety-specific marker of the present invention can be used to identify rice varieties. Examples of cultivar identification are as follows; identifying rice varieties included in the sample; identifying rice varieties not included in the sample; 1 varieties included in the sample Confirm whether it is a species, or two or more species; Confirm whether the rice varieties included in the sample are one of a specific variety group; Confirm whether or not the product is actually included in the sample (for example, the authenticity of the product labeling); whether or not other samples are included in the sample that includes the specific product To check.
In the present invention, the rice variety-specific marker may be one or more selected from the group consisting of polymorphisms (1) to (48), and one or more may be selected and used according to the purpose. . In some cases, it is not possible to specify a single variety by using only one species. However, if the variety included in the sample can be predicted to some extent, only the polymorphism need be used.

  Examples of rice varieties that can be identified in the present invention are as follows: Koshihikari, Hitomebore, Akitakomachi, Hinohikari, Nanatsuboshi, Haenuki, Kirara 397, Shinigura, Shinobuki, Tsumagome, Kinuhikari, Asahi Dream , Yumepirika, Fusakogan, Tsuyahime, Hanaechizen, Yume Tsukushi, Fusotome, Fukinko, Hoshino Yume, Menkoina, Kinusume, Aichi no Kaori, Orozuki, Yume Mizuho, Sasanishiki, Akebono, Milky Queen, Yume Oba Koshiko, Hatsushimo, Tentaku, Nihonbare, Aya no Kagayaki, Nikkoru, Yume Shizuku, Yukinko Mai, Sabiyori, Nasumikari, Manamusume, Autumn Poetry, Genki Shishi, Mori no Kumasan, Chiyonishiki, Akisakari , Asahi, Hiyokumochi, Himenomochi, Koganemochi, Kokomochimochi, Wataboshi, Hakucho Chi, stand Whirlpool, Kita Yukimochi, Kinunohada, Nishiki Yamada, five Hyakumangoku. In addition to the rice varieties described above, if there are varieties that exhibit polymorphism similar to that cultivar, such as varieties that are closely related to any of these, such varieties can also be identified in the present invention.

  The polymorphisms in the rice variety-specific markers of the present invention in the above rice varieties are shown in Tables 2 to 5.

  In the present invention, rice varieties can be identified based on polymorphism data in each rice variety shown in Tables 2 to 5 by confirming the insertion site of the marker in the genome of the test rice. In the present invention, it is not necessary to confirm the insertion site for all of the polymorphisms (1) to (48). For example, when mPing is inserted into the insertion site of the polymorphic (4) mPing, it is determined that it is Nanatsubo.

  In the present invention, it is not always necessary to determine the insertion of mPing in a rice variety-specific marker, and the nucleotide sequences of polymorphisms (1) to (48) in the genome of the test rice are the nucleotide sequences of corresponding sites in Nipponbare The rice varieties can be identified by checking whether they are the same.

  Tables 6 and 7 below show examples of combinations of variety-specific markers that can identify each of the above-mentioned rice varieties. It is not necessarily limited to the combinations of markers listed in Tables 6 and 7. From the information on the presence or absence of mPing insertion in the rice genome polymorphisms (1) to (48) of each variety, the combination of markers that can be used for variety identification is appropriately selected. It is possible to select.

  In the present invention, when rice varieties are identified using a cultivar identification marker, the presence or absence of mPing in any of polymorphisms (1) to (48) in the rice genome contained in the sample is determined. That's fine. In addition, even if the presence or absence of mPing is not clearly determined, it can be determined that mPing is not inserted if the rice genome and the corresponding Nipponbare genome contained in the sample are compared with each other and the corresponding regions are the same. Further, if both are different, it may be determined that they are inserted.

  In the present invention, for example, the method comprises hybridizing a marker for identifying the variety of the present invention, that is, a probe capable of detecting the presence or absence of mPing in polymorphisms (1) to (48), or two or more primers. An amplification reaction using at least a primer set can be used, of which the latter is preferred.

  The probe is an oligonucleotide complementary to the base sequence in the vicinity of the mPing insertion site of any of polymorphisms (1) to (48), and may be modified with a fluorescent substance or the like as necessary.

  The amplification reaction is not particularly limited as long as a test by amplification of DNA using two or more primers is possible, and examples thereof include an amplification reaction under isothermal conditions and an amplification reaction under non-isothermal conditions. Examples of the amplification reaction under isothermal conditions include, for example, the LAMP (Loop-Mediated Isometric Amplification) method, the ICAN (Isothermal and Chimeric Primer-initiated Amplified of Nucleic Acids Amplified, Amplified Amplified, Amplified Acid, Amplified of Amplified Acid, Amplified Amplified Acid, Amplified Amplified Acid, Amplified Amplified Acid, Amplified Amplified Acid. (Amplification) method. An example of the amplification reaction under non-isothermal conditions is a PCR method. In the present invention, the amplification reaction is preferably the LAMP method or the PCR method.

  In the method of the present invention, the sample subjected to the amplification reaction is a sample containing or possibly containing genomic DNA derived from rice (Oryza Sativa). Rice may be a plant body, a part thereof or a processed product thereof. Examples of the plant body include rice (seed), leaves, stems, flowers, roots, etc. Of these, rice is preferred. The rice may be either brown rice or polished rice. The rice may also be any of japonica rice, jabonica rice and indica rice, and may be either sticky rice, glutinous rice or sake rice. Examples of processed rice products include cooked rice, processed rice, miso, rice confectionery, rice flour, rice vinegar, rice cake, rice noodles, brown rice tea, rice flour bread, rice flour noodles, sake and beer.

  The primer set used in the method of the present invention can detect the presence or absence of insertion of mPing in the rice cultivar identification marker, ie, a polymorphism selected from the group consisting of polymorphisms (1) to (48). The primer set comprised from the above primer can be mentioned.

  The number of primers included in each primer set varies depending on the amplification reaction employed, but is, for example, 2 to 6.

  The number of nucleotide residues constituting the primer (the nucleotide residue of the complementary portion that anneals to the target site) is not particularly limited as long as the primer can anneal to the target site and specifically amplify the target product. It may be 16 or more, 17 or more, 18 or more, or 20 or more. The number of nucleotide residues constituting the primer may be, for example, 50 or less, 40 or less, or 30 or less.

  The primer may be labeled with a substance such as a fluorescent substance in order to easily detect the amplification product. Examples of such fluorescent materials include 6-FAM, VIC, NED, and PET. Of course, the primer need not necessarily be labeled with such a substance. For example, the difference in size of amplification products may be confirmed by gel electrophoresis. In the detection by the LAMP method, the presence or absence of amplification can be confirmed by visually confirming the white turbidity of magnesium pyrophosphate, which is a byproduct of the amplification reaction. In such a case, it is not necessary to label the primer with a substance such as a fluorescent substance.

When detecting by the LAMP method, a primer set for LAMP may be used. The LAMP primer set is composed of i) to iv) below (see FIG. 49).
i) FIP (Forward Inner Primer): designed to have an F2 region complementary to the F2c region of the target DNA at the 3 ′ end and the same sequence as the F1c region of the target DNA at the 5 ′ end;
ii) F3 primer: designed to have an F3 region complementary to the F3c region of the target DNA;
iii) BIP (Backward Inner Primer): designed to have a B2 region complementary to the B2c region of the target DNA at the 3 ′ end and the same sequence as the B1c region of the target DNA at the 5 ′ end;
iv) B3 primer: designed to have a B3 region complementary to the B3c region of the target DNA.

Preferably, the LAMP primer set may further include the following loop primers (see FIG. 49).
v) LF (Loop Primer F): designed to have a complementary sequence in the part between the F1 region and the F2 region;
vi) LB (Loop Primer B): designed to have a complementary sequence in the portion between the B1 and B2 regions;
Can do.

  Hereinafter, the specific primer set used suitably by the method of this invention is explained in full detail.

<1> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (1)

  In the base sequence of SEQ ID NO: 1, the mPing insertion region is a region consisting of nucleotide residues 702 to 1131 (FIG. 1). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (1) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

The primer set for the first target mPing of the polymorphism (1) includes a combination of primers composed of the following primer pairs (eg, a primer set for PCR), and three or more primer sets including such primer combinations (For example, a LAMP primer set using at least four primers), a PCR primer set and a LAMP primer set are preferred:
<1a> a first primer having at least a partial base sequence of the 1st to 701st base sequences in the base sequence of SEQ ID NO: 1 or an equivalent base sequence thereof, and 1132 to 1890th in the base sequence of SEQ ID NO: 1 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<1b> a first primer having at least a partial base sequence of the 1st to 701st base sequences in the base sequence of SEQ ID NO: 1 or a complementary sequence of the equivalent base sequence, and 1132 in the base sequence of SEQ ID NO: 1 A combination of second primers having at least a part of the base sequence of the 1890th base sequence or an equivalent base sequence thereof;
<1c> a first primer having at least a partial base sequence of the 1st to 701st base sequences in the base sequence of SEQ ID NO: 1 or an equivalent base sequence thereof, and the 702th to 1131st positions of the base sequence of SEQ ID NO: 1 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<1d> a first primer having at least a partial base sequence of the 1st to 701th base sequences in the base sequence of SEQ ID NO: 1 or a complementary sequence thereof, and 702 in the base sequence of SEQ ID NO: 1 A combination of second primers having at least a part of the base sequence of the 1131st base sequence or an equivalent base sequence thereof;
<1e> a first primer having at least a partial base sequence of the 702 to 1131th base sequence in the base sequence of SEQ ID NO: 1 or an equivalent base sequence thereof, and the 1132 to 1890th base sequences of the base sequence of SEQ ID NO: 1 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<1f> a first primer having at least a partial base sequence of the 702 to 1131th base sequence in the base sequence of SEQ ID NO: 1 or a complementary sequence of the equivalent base sequence, and 1132 in the base sequence of SEQ ID NO: 1 A combination of second primers having at least a part of the base sequence of the 1890th base sequence or an equivalent base sequence thereof.

The LAMP primer set that can detect the presence or absence of insertion of the polymorphism (1) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<1a ′> A base sequence of at least a part of the 1st to 701st base sequences in the base sequence of SEQ ID NO: 1 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1132 to 1890 in the base sequence of SEQ ID NO: 1 or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<1b ′> A base sequence of at least a part of the 1st to 701st base sequences in the base sequence of SEQ ID NO: 1 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1132 to 1890th base sequence in the base sequence of SEQ ID NO: 1 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<1c ′> The base sequence of at least a part of the 1st to 701st base sequences in the base sequence of SEQ ID NO: 1 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 702 to 1131 in the base sequence of SEQ ID NO: 1 or a complementary sequence of the equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<1d ′> At least a part of the 1st to 701st nucleotide sequences in the nucleotide sequence of SEQ ID NO: 1 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 702 to 1131st base sequence in the base sequence of SEQ ID NO: 1 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<1e ′> At least a part of the 702 to 1131st nucleotide sequence in the nucleotide sequence of SEQ ID NO: 1 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1132 to 1890 in the base sequence of SEQ ID NO: 1 or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<1f ′> A base sequence of at least a part of the 702 to 1131st base sequences in the base sequence of SEQ ID NO: 1 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1132 to 1890th base sequence in the base sequence of SEQ ID NO: 1 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <1a> to <1f> and <1a ′> to <1f ′>, “the 1st to 701st base sequence in the base sequence of SEQ ID NO: 1” is “the 1st to 701st base in the base sequence of SEQ ID NO: 61” In “Sequence”, “the 1132 to 1890th base sequence in the base sequence of SEQ ID NO: 1” may be replaced with “the 702 to 1457th base sequence in the base sequence of SEQ ID NO: 61”, respectively.

  In addition to the four primer combinations <1a ′> to <1f ′>, two loop primers (LF and LB) may be used in combination.

  The four primer combinations <1a ′> to <1f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <1a ′> to <1b ′> and any of <1c ′> to <1f ′>.

  A first preferred example of a primer set for LAMP capable of detecting mPing in the polymorphism (1) is a primer consisting of the base sequence of SEQ ID NO: 51, a primer consisting of the base sequence of SEQ ID NO: 52, and a base sequence of SEQ ID NO: 53 And a primer consisting of the base sequence of SEQ ID NO: 54.

  In the first preferred example, it is more preferable that the LAMP primer set for the first target mPing further includes a primer consisting of the base sequence of SEQ ID NO: 55 and a base sequence of SEQ ID NO: 56 corresponding to the loop primer. . Therefore, in the present invention, the 35P primer set described in the examples is preferably used.

  A second preferred example of the LAMP primer set for detecting mPing in the polymorphism (1) consists of a primer consisting of the base sequence of SEQ ID NO: 51, a primer consisting of the base sequence of SEQ ID NO: 57, and a base sequence of SEQ ID NO: 53 And a primer consisting of the base sequence of SEQ ID NO: 58.

  In the first preferred example, the LAMP primer set for the first target mPing further preferably includes a primer consisting of the base sequence of SEQ ID NO: 59 and a base sequence of SEQ ID NO: 60 corresponding to the loop primer. . Therefore, in the present invention, the 35N primer set described in the examples is also preferably used.

<2> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (2);
In the base sequence of SEQ ID NO: 2, the insertion region of mPing is a region consisting of the 674th to 1103rd nucleotide residues (FIG. 2). Therefore, as two or more primers for detecting the presence or absence of insertion of mPing of polymorphism (2), a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

As a primer set for mPing of polymorphism (2), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<2a> a first primer having at least a part of the 1-673th base sequence in the base sequence of SEQ ID NO: 2 or an equivalent base sequence thereof, and the 1104th to 1918th positions in the base sequence of SEQ ID NO: 2 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<2b> a first primer having at least a partial base sequence of the 1st to 673th base sequence in the base sequence of SEQ ID NO: 2 or a complementary sequence thereof, and 1104 to 1 in the base sequence of SEQ ID NO: 2 A combination of second primers having at least a part of the base sequence of the 1918th base sequence or an equivalent base sequence thereof;
<2c> a first primer having at least a part of a base sequence of 1 to 673 in the base sequence of SEQ ID NO: 2 or an equivalent base sequence thereof, and 674 to 1103 in the base sequence of SEQ ID NO: 2 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<2d> a first primer having at least a partial base sequence of the 1st to 673th base sequences in the base sequence of SEQ ID NO: 2 or a complementary sequence thereof, and 674 to in the base sequence of SEQ ID NO: 2 A combination of second primers having at least a part of the base sequence of the 1103rd base sequence or an equivalent base sequence thereof;
<2e> a first primer having at least a partial base sequence of the 674 to 1103rd base sequence in the base sequence of SEQ ID NO: 2 or an equivalent base sequence thereof, and the 1104 to 1918th base sequences of the base sequence of SEQ ID NO: 2 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<2f> a first primer having at least a partial base sequence of the 674 to 1103rd base sequence in the base sequence of SEQ ID NO: 2 or a complementary sequence of the equivalent base sequence, and 1104 in the base sequence of SEQ ID NO: 2 The combination of the 2nd primer which has at least one part base sequence of the 1918th base sequence, or its equivalent base sequence.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (2) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<2a ′> A base sequence of at least a part of the 1st to 673rd base sequences in the base sequence of SEQ ID NO: 2 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1104 to 1918th base sequence in the base sequence of SEQ ID NO: 2 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<2b ′> At least a part of the 1-673th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 2 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 1104 to 1918th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 2 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<2c ′> A base sequence of at least a part of the 1st to 673rd base sequences in the base sequence of SEQ ID NO: 2 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the 674 to 1103 base sequence in the base sequence of SEQ ID NO: 2 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<2d ′> At least a part of the 1-673th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 2 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 674 to 1103rd base sequence in the base sequence of SEQ ID NO: 2 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<2e ′> At least a part of the 674 to 1103rd base sequence in the base sequence of SEQ ID NO: 2 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1104 to 1918th base sequence in the base sequence of SEQ ID NO: 2 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<2f ′> A base sequence of at least a part of the 674th to 1103rd base sequences in the base sequence of SEQ ID NO: 2 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 1104 to 1918th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 2 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <2a> to <2f> and <2a ′> to <2f ′>, “the base sequence of at least a part of the 1st to 673rd base sequences in the base sequence of SEQ ID NO: 2” is “the base sequence of SEQ ID NO: 62” "At least a part of the base sequence of the 1-673th base sequence", "the 1104-1918th base sequence in the base sequence of SEQ ID NO: 2" is "the 674-1485th base sequence in the base sequence of SEQ ID NO: 62" May be replaced respectively.

  In addition to the combination of the four primers <2a ′> to <2f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <2a ′> to <2f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <2a ′> to <2b ′> and any of <2c ′> to <2f ′>.

<3> Primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (3) In the base sequence of SEQ ID NO: 3, the insertion region of mPing is the 640th to 1069th nucleotide residues (FIG. 3). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (3) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

As a primer set for mPing of polymorphism (3), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<3a> a first primer having at least a partial base sequence of the 1st to 639th base sequences in the base sequence of SEQ ID NO: 3 or an equivalent base sequence thereof, and the 1070th to 1755th positions of the base sequence of SEQ ID NO: 3 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<3b> a first primer having at least a part of the 1-639th base sequence in the base sequence of SEQ ID NO: 3 or a complementary sequence of the equivalent base sequence, and 1070 in the base sequence of SEQ ID NO: 3 A combination of second primers having at least a part of the base sequence of the 1755th base sequence or an equivalent base sequence thereof;
<3c> a first primer having at least a part of the 1-639th base sequence in the base sequence of SEQ ID NO: 3 or an equivalent base sequence thereof, and the 640th to 1069th positions in the base sequence of SEQ ID NO: 3 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<3d> a first primer having at least a partial base sequence of the 1st to 639th base sequences in the base sequence of SEQ ID NO: 3 or a complementary sequence thereof, and 640 to 400 in the base sequence of SEQ ID NO: 3 A second primer combination having at least a part of the base sequence of the 1069th base sequence or an equivalent base sequence thereof;
<3e> a first primer having at least a partial base sequence of the 640th to 1069th base sequences in the base sequence of SEQ ID NO: 3 or an equivalent base sequence thereof, and the 1070th to 1755th positions in the base sequence of SEQ ID NO: 3 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<3f> a first primer having at least a part of the base sequence of the 640th to 1069th base sequences in the base sequence of SEQ ID NO: 3 or a complementary sequence of the equivalent base sequence, and 1070 in the base sequence of SEQ ID NO: 3 A combination of second primers having at least a part of the 1755th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (3) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<3a ′> At least a part of the 1-639th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 3 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of the 1070 to 1755th base sequence in the base sequence of SEQ ID NO: 3 or a complementary sequence of the equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<3b ′> At least part of the 1-639th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 3 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1070 to 1755th base sequence in the base sequence of SEQ ID NO: 3 or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<3c ′> At least a part of the 1-639th base sequence in the base sequence of SEQ ID NO: 3 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 640 to 1069 in the base sequence of SEQ ID NO: 3 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<3d ′> At least a part of the 1-639th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 3 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 640th to 1069th base sequences in the base sequence of SEQ ID NO: 3 or an equivalent base sequence thereof A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<3e ′> At least a part of the 640th to 1069th base sequences in the base sequence of SEQ ID NO: 3 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of the 1070 to 1755th base sequence in the base sequence of SEQ ID NO: 3 or a complementary sequence of the equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<3f ′> A base sequence of at least a part of the 640th to 1069th base sequences in the base sequence of SEQ ID NO: 3 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1070 to 1755th base sequence in the base sequence of SEQ ID NO: 3 or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <3a> to <3f> and <3a ′> to <3f ′>, “the 1st to 639th base sequence in the base sequence of SEQ ID NO: 3” is “the 1st to 639th base in the base sequence of SEQ ID NO: 63” In the “sequence”, “the 1070th to 1755th base sequence in the base sequence of SEQ ID NO: 3” may be replaced with “the 640th to 1322th base sequence in the base sequence of SEQ ID NO: 63”, respectively.

  In addition to the four primer combinations <3a ′> to <3f ′>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <3a ′> to <3f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <3a '> to <3b'> and any of <3c '> to <3f'>.

<4> A primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (4);
In the base sequence of SEQ ID NO: 4, the mPing insertion region is a region consisting of nucleotide residues 731 to 1160 (FIG. 4). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (4) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

The primer set for the polymorphism (4) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<4a> a first primer having at least a partial base sequence of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 4 or an equivalent base sequence thereof, and the 1161st to 1810th positions of the base sequence of SEQ ID NO: 4 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<4b> a first primer having at least a partial base sequence of the 1st to 730th base sequence in the base sequence of SEQ ID NO: 4 or a complementary sequence thereof, and 1161 in the base sequence of SEQ ID NO: 4 A combination of second primers having at least a part of the base sequence of the 1810th base sequence or an equivalent base sequence thereof;
<4c> a first primer having at least a partial base sequence of the 1st to 730th base sequence in the base sequence of SEQ ID NO: 4 or an equivalent base sequence thereof, and the 731st to 1160th base sequence of the base sequence of SEQ ID NO: 4 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<4d> a first primer having at least a partial base sequence of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 4 or a complementary sequence thereof, and 731-1 in the base sequence of SEQ ID NO: 4 A combination of second primers having at least a part of the base sequence of the 1160th base sequence or an equivalent base sequence thereof;
<4e> a first primer having at least a partial base sequence of the 731st to 1160th base sequences in the base sequence of SEQ ID NO: 4 or an equivalent base sequence thereof, and the 1161st to 1810th positions of the base sequence of SEQ ID NO: 4 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<4f> a first primer having at least a partial base sequence of the 731st to 1160th base sequence in the base sequence of SEQ ID NO: 4 or a complementary sequence of the equivalent base sequence, and 1161 in the base sequence of SEQ ID NO: 4 The combination of the 2nd primer which has at least one part base sequence of the 1810th base sequence, or its equivalent base sequence.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (4) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<4a ′> At least a part of the 1-730th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 4 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the 1161-1810th base sequence in the base sequence of SEQ ID NO: 4 or a complementary sequence equivalent thereto, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<4b ′> At least a part of the 1st to 730th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 4 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the 1161-1810th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 4 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<4c ′> At least a part of the 1-730th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 4 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 731 to 1160 in the base sequence of SEQ ID NO: 4 or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<4d ′> At least a part of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 4 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 731st to 1160th base sequences in the base sequence of SEQ ID NO: 4 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<4e ′> A base sequence of at least a part of the 731st to 1160th base sequences in the base sequence of SEQ ID NO: 4 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the 1161-1810th base sequence in the base sequence of SEQ ID NO: 4 or a complementary sequence equivalent thereto, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<4f ′> At least a part of the 731 to 1160th base sequence in the base sequence of SEQ ID NO: 4 or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the 1161-1810th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 4 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <4a> to <4f> and <4a ′> to <4f ′>, “the base sequence of at least a part of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 4” is “the base sequence of SEQ ID NO: 64” "At least a part of the base sequence of the 1st to 730th base sequence" in "the 1161st to 1810th base sequence in the base sequence of SEQ ID NO: 4" is "the 731st to 1377th base sequence in the base sequence of SEQ ID NO: 64" May be replaced respectively.

  In addition to the four primer combinations <4a ′> to <4f ′>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <4a '> to <4f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <4a ′> to <4b ′> and any of <4c ′> to <4f ′>.

<5> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (5) In the base sequence of SEQ ID NO: 5, the insertion region of mPing is the 693-1122nd nucleotide residue (FIG. 5). Therefore, as two or more primers for detecting the presence or absence of insertion of mPing of polymorphism (5), a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

The primer set for the polymorphism (5) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<5a> a first primer having at least a part of the base sequence of 1 to 692 in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof, and 1123 to 1817th in the base sequence of SEQ ID NO: 5 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<5b> a first primer having at least a part of a base sequence of 1 to 692 in the base sequence of SEQ ID NO: 5 or a complementary sequence of the equivalent base sequence, and 1123 in the base sequence of SEQ ID NO: 5 A combination of second primers having at least a part of the base sequence of the 1817th base sequence or an equivalent base sequence thereof;
<5c> a first primer having at least a part of the base sequence of 1 to 692 in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof, and the 693 to 1122 th in the base sequence of SEQ ID NO: 5 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<5d> a first primer having at least a part of the base sequence of 1 to 692 in the base sequence of SEQ ID NO: 5 or a complementary sequence of the equivalent base sequence, and 693 in the base sequence of SEQ ID NO: 5 A combination of second primers having at least part of the 1122nd base sequence or an equivalent base sequence thereof;
<5e> a first primer having at least a part of the base sequence of 693 to 1122 in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof, and 1123 to 1817 in the base sequence of SEQ ID NO: 5 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<5f> a first primer having at least a partial base sequence of the 693-1112th base sequence in the base sequence of SEQ ID NO: 5 or a complementary sequence of the equivalent base sequence, and 1123 in the base sequence of SEQ ID NO: 5 The combination of the 2nd primer which has at least one part base sequence of the 1817th base sequence, or its equivalent base sequence.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (5) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<5a ′> The base sequence of at least a part of the 1st to 692nd base sequences in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence from 1123 to 1817 in the base sequence of SEQ ID NO: 5 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<5b ′> At least a part of the base sequence of the first to 692 base sequences in the base sequence of SEQ ID NO: 5 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1123 to 1817th base sequences in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<5c ′> At least a part of the base sequence of the first to 692 base sequences in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 693 to 1122 in the base sequence of SEQ ID NO: 4, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<5d ′> At least a part of the base sequence of 1 to 692 in the base sequence of SEQ ID NO: 5 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 693 to 1122 base sequences in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<5e ′> At least a part of the 693 to 1122 base sequences in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence from 1123 to 1817 in the base sequence of SEQ ID NO: 5 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<5f ′> A base sequence of at least a part of the 693 to 1122 base sequences in the base sequence of SEQ ID NO: 5 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1123 to 1817th base sequences in the base sequence of SEQ ID NO: 5 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <5a> to <5f> and <5a ′> to <5f ′>, “1st to 692nd base sequence in the base sequence of SEQ ID NO: 5” is “1st to 692nd base in the base sequence of SEQ ID NO: 65” In the “sequence”, “the 1123-1817th base sequence in the base sequence of SEQ ID NO: 5” may be replaced with the “693-1384th base sequence in the base sequence of SEQ ID NO: 65”.

  In addition to the four primer combinations <5a '> to <5f'>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <5a '> to <5f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <5a '> to <5b'> and any of <5c '> to <5f'>.

<6> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (6);
In the base sequence of SEQ ID NO: 6, the insertion region of mPing is a region consisting of nucleotide residues 737 to 1166 (FIG. 6). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (6) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

As a primer set for mPing of polymorphism (6), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<6a> a first primer having at least a part of the 1-736th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 6 or an equivalent nucleotide sequence thereof, and the 1167th to 1829th nucleotides in the nucleotide sequence of SEQ ID NO: 6 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<6b> a first primer having at least a partial base sequence of the 1-736th base sequence in the base sequence of SEQ ID NO: 6 or a complementary sequence thereof, and 1167-in the base sequence of SEQ ID NO: 6 A combination of second primers having at least a part of the base sequence of the 1829th base sequence or an equivalent base sequence thereof;
<6c> a first primer having at least a part of the 1-736th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 6 or an equivalent nucleotide sequence thereof, and the 737-1166th nucleotide in the nucleotide sequence of SEQ ID NO: 6 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<6d> a first primer having at least a partial base sequence of the 1st to 736th base sequences in the base sequence of SEQ ID NO: 6 or a complementary sequence thereof, and 737 in the base sequence of SEQ ID NO: 6 A combination of second primers having at least part of the 1166th base sequence or an equivalent base sequence thereof;
<6e> a first primer having at least a partial base sequence of the 737 to 1166th base sequence in the base sequence of SEQ ID NO: 6 or an equivalent base sequence thereof, and the 1167th to 1829th positions of the base sequence of SEQ ID NO: 6 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<6f> a first primer having at least a part of a base sequence of the 737th to 1166th base sequences in the base sequence of SEQ ID NO: 6 or a complementary sequence thereof, and 1167 to the base sequence of SEQ ID NO: 6 A combination of second primers having at least a part of the base sequence of the 1829th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (6) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<6a ′> At least a part of the 1-736th base sequence in the base sequence of SEQ ID NO: 6 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1167 to 1829 in the base sequence of SEQ ID NO: 6 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<6b ′> At least a part of the 1-736th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 6 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1167th to 1829th base sequences in the base sequence of SEQ ID NO: 6 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<6c ′> At least a part of the 1-736th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 6 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably 1st primer having (as F2 region in FIP), and at least a part of the base sequence of 737 to 1166th base sequence in the base sequence of SEQ ID NO: 6 or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<6d '> A base sequence of at least a part of the 1st to 736th base sequences in the base sequence of SEQ ID NO: 6 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 737 to 1166th base sequence in the base sequence of SEQ ID NO: 6 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<6e ′> At least a part of the base sequence of the 737 to 1166th base sequence in the base sequence of SEQ ID NO: 6 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1167 to 1829 in the base sequence of SEQ ID NO: 6 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<6f ′> At least a part of the base sequence of the 737th to 1166th base sequences in the base sequence of SEQ ID NO: 6 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1167th to 1829th base sequences in the base sequence of SEQ ID NO: 6 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <6a> to <6f> and <6a ′> to <6f ′>, “the 1st to 736th base sequence in the base sequence of SEQ ID NO: 6” is “the 1st to 736th base in the base sequence of SEQ ID NO: 66” In the “sequence”, “the 1167th to 1829th base sequence in the base sequence of SEQ ID NO: 6” may be replaced with “the 737th to 1396th base sequence in the base sequence of SEQ ID NO: 66”.

  In addition to the combination of the four primers <6a ′> to <6f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <6a '> to <6f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <6a ′> to <6b ′> and any of <6c ′> to <6f ′>.

<7> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (7) In the base sequence of SEQ ID NO: 7, the insertion region of mPing is the nucleotide residues 713 to 1142 (FIG. 7). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (7) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

As a primer set for the polymorphism (7) mPing, a primer combination composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<7a> a first primer having at least a part of a base sequence of 1 to 712 in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof, and 1143 to 1896 of the base sequence of SEQ ID NO: 3 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<7b> a first primer having at least a partial base sequence of the 1st to 712th base sequences in the base sequence of SEQ ID NO: 7 or a complementary sequence thereof, and 1143 to 1143 in the base sequence of SEQ ID NO: 7 A second primer combination having at least a part of the base sequence of the 1896th base sequence or an equivalent base sequence thereof;
<7c> a first primer having at least a part of the 1st to 712th base sequence in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof, and the 713th to 1142st positions in the base sequence of SEQ ID NO: 7 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<7d> a first primer having at least a partial base sequence of the 1st to 712th base sequences in the base sequence of SEQ ID NO: 7 or a complementary sequence thereof, and 713- 3 in the base sequence of SEQ ID NO: 7 A combination of second primers having at least part of the 1142nd base sequence or an equivalent base sequence thereof;
<7e> a first primer having at least a part of a base sequence of 713 to 1142 in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof, and 1143 to 1896 of the base sequence of SEQ ID NO: 7 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<7f> a first primer having at least a part of the base sequence of 713 to 1142 in the base sequence of SEQ ID NO: 7 or a complementary sequence of the equivalent base sequence, and 1143 to 1143 in the base sequence of SEQ ID NO: 7 A combination of second primers having at least a part of the 1896th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (7) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<7a ′> A base sequence of at least a part of the 1st to 712th base sequences in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1143 to 1896 in the base sequence of SEQ ID NO: 7 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<7b ′> A base sequence of at least a part of the 1st to 712th base sequences in the base sequence of SEQ ID NO: 7 or a complementary sequence of the equivalent base sequence is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1143 to 1896th base sequence in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<7c ′> A base sequence of at least a part of the 1st to 712th base sequences in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 713 to 1142 in the base sequence of SEQ ID NO: 7 or a complementary sequence equivalent thereto, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<7d ′> At least a part of the 1st to 712th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 7 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of 713 to 1142 in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<7e ′> At least a part of the base sequence of 713 to 1142 in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1143 to 1896 in the base sequence of SEQ ID NO: 7 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<7f ′> A base sequence of at least a part of the 713th to 1142nd base sequences in the base sequence of SEQ ID NO: 7 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1143 to 1896th base sequence in the base sequence of SEQ ID NO: 7 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <7a> to <7f> and <7a ′> to <7f ′>, “the 1st to 712th base sequence in the base sequence of SEQ ID NO: 7” is “the 1st to 712th base in the base sequence of SEQ ID NO: 67” In the “sequence”, “the 1143 to 1896th base sequence in the base sequence of SEQ ID NO: 7” may be replaced with “the 713th to 1463th base sequence in the base sequence of SEQ ID NO: 67”, respectively.

  In addition to the four primer combinations <7a '> to <7f'>, two loop primers (LF and LB) may be further used in combination.

  The four primer combinations <7a '> to <7f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <7a ′> to <7b ′> and any of <7c ′> to <7f ′>.

<8> A primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (8);
In the base sequence of SEQ ID NO: 8, the mPing insertion region is a region consisting of nucleotide residues 686 to 1115 (FIG. 8). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (8) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

As a primer set for mPing of polymorphism (8), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<8a> a first primer having at least a part of the 1st to 685th base sequences in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof, and the 1116th to 1928th positions of the base sequence of SEQ ID NO: 8 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<8b> a first primer having at least a partial base sequence of the 1st to 685th base sequences in the base sequence of SEQ ID NO: 8 or a complementary sequence thereof, and 1116 to 1 in the base sequence of SEQ ID NO: 8 A second primer combination having at least a part of the base sequence of the 1928th base sequence or an equivalent base sequence thereof;
<8c> a first primer having at least a part of the 1st to 685th base sequence in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof, and the 686th to 1115th positions in the base sequence of SEQ ID NO: 8 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<8d> a first primer having at least a partial base sequence of the 1st to 685th base sequences in the base sequence of SEQ ID NO: 8 or a complementary sequence thereof, and 686 in the base sequence of SEQ ID NO: 8 A combination of second primers having at least a part of the base sequence of the 1115th base sequence or an equivalent base sequence thereof;
<8e> a first primer having at least a part of the base sequence of 686 to 1115 in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof, and the 1116th to 1928th positions of the base sequence of SEQ ID NO: 8 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<8f> A first primer having at least a partial base sequence of the 686th to 1115th base sequence in the base sequence of SEQ ID NO: 8 or a complementary sequence thereof, and 1116 to in the base sequence of SEQ ID NO: 8 A combination of second primers having at least a part of the 1928th base sequence or an equivalent base sequence thereof.

The LAMP primer set that can detect the presence or absence of insertion of the polymorphism (8) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<8a ′> A base sequence of at least a part of the 1st to 685th base sequences in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1116th to 1928th base sequences in the base sequence of SEQ ID NO: 8, or a complementary sequence of the equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<8b ′> A base sequence of at least a part of the 1st to 685th base sequences in the base sequence of SEQ ID NO: 8 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1116th to 1928th base sequences in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<8c ′> At least a part of the 1st to 685th base sequences in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 686 to 1115 in the base sequence of SEQ ID NO: 8 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<8d ′> A base sequence of at least a part of the 1st to 685th base sequences in the base sequence of SEQ ID NO: 8 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 686th to 1115th base sequences in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<8e ′> At least a part of the 686 to 1115th base sequence in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1116th to 1928th base sequences in the base sequence of SEQ ID NO: 8, or a complementary sequence of the equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<8f ′> A base sequence of at least a part of the 686th to 1115th base sequences in the base sequence of SEQ ID NO: 8 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1116th to 1928th base sequences in the base sequence of SEQ ID NO: 8 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <8a> to <8f> and <8a ′> to <8f ′>, “the 1st to 685th base sequence in the base sequence of SEQ ID NO: 8” is “the 1st to 685th base in the base sequence of SEQ ID NO: 68” In the “sequence”, “the 1116th to 1928th base sequence in the base sequence of SEQ ID NO. 8” may be replaced with “the 686th to 1495th base sequence in the base sequence of SEQ ID NO. 68”, respectively.

  In addition to the combination of the four primers <8a ′> to <8f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <8a '> to <8f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <8a ′> to <8b ′> and any of <8c ′> to <8f ′>.

<9> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (9) In the base sequence of SEQ ID NO: 9, the insertion region of mPing is the 739th to 1168th nucleotide residues (FIG. 9). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (9) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination (increased) having a base sequence corresponding to this region (sense strand or antisense strand) Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of (see FIG. 50).

As a primer set for the polymorphism (9) mPing, a primer combination comprising the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<9a> a first primer having at least a part of the 1-738th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 9 or an equivalent nucleotide sequence thereof, and the 1169th-1886th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 9 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<9b> a first primer having at least a partial base sequence of the 1-738th base sequence in the base sequence of SEQ ID NO: 9 or a complementary sequence of the equivalent base sequence, and 1169- in the base sequence of SEQ ID NO: 9 A second primer combination having at least a part of the base sequence of the 1886th base sequence or an equivalent base sequence thereof;
<9c> a first primer having at least a partial base sequence of the 1-738th base sequence in the base sequence of SEQ ID NO: 9 or an equivalent base sequence thereof, and the 739-1168th base sequence of the base sequence of SEQ ID NO: 9 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<9d> a first primer having at least a partial base sequence of the 1st to 738th base sequences in the base sequence of SEQ ID NO: 9 or a complementary sequence thereof, and 739 to 7 in the base sequence of SEQ ID NO: 9 A combination of second primers having at least a part of the 1168th base sequence or an equivalent base sequence thereof;
<9e> a first primer having at least a partial base sequence of the 739 to 1168th base sequence in the base sequence of SEQ ID NO: 9 or an equivalent base sequence thereof, and the 1169th to 1886th base sequences of the base sequence of SEQ ID NO: 9 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<9f> A first primer having at least a part of the 739 to 1168th base sequence in the base sequence of SEQ ID NO: 9 or a complementary sequence of the equivalent base sequence, and 1169 to 1169 in the base sequence of SEQ ID NO: 9 A combination of second primers having at least a part of the base sequence of the 1886th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (9) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<9a ′> At least a part of the 1-738th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 9 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence from 1169 to 1886 in the base sequence of SEQ ID NO: 9 or a complementary sequence thereof equivalent to the base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<9b ′> At least a part of the 1-738th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 9 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 1169 to 1886th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 9 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<9c ′> A base sequence of at least a part of the 1-738th base sequence in the base sequence of SEQ ID NO: 9 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 739 to 1168 in the base sequence of SEQ ID NO: 4 or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<9d ′> At least a part of the 1-738th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 9 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 739 to 1168th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 9 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<9e ′> At least a part of the 739 to 1168th base sequence in the base sequence of SEQ ID NO: 9 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence from 1169 to 1886 in the base sequence of SEQ ID NO: 9 or a complementary sequence thereof equivalent to the base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<9f ′> A base sequence of at least a part of the 739th to 1168th base sequence in the base sequence of SEQ ID NO: 9 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 1169 to 1886th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 9 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <9a> to <9f> and <9a ′> to <9f ′>, “the 1st to 738th base sequence in the base sequence of SEQ ID NO: 9” is “the 1st to 738th base in the base sequence of SEQ ID NO: 69” In the “sequence”, “the 1169th to 1886th base sequence in the base sequence of SEQ ID NO: 9” may be replaced by “the 739th to 1453th base sequence in the base sequence of SEQ ID NO: 69”.

  In addition to the four primer combinations <9a '> to <9f'>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <9a '> to <9f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <9a '> to <9b'> and any of <9c '> to <9f'>.

<10> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (10);
In the base sequence of SEQ ID NO: 10, the mPing insertion region is a region consisting of nucleotide residues 734 to 1163 (FIG. 10). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (10) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (10) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<10a> a first primer having at least a part of the 1-733th base sequence in the base sequence of SEQ ID NO: 10 or an equivalent base sequence thereof, and the 1164th to 1741th positions in the base sequence of SEQ ID NO: 10 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<10b> a first primer having at least a partial base sequence of the 1st to 733th base sequence in the base sequence of SEQ ID NO: 10 or a complementary sequence of the equivalent base sequence, and 1164-in the base sequence of SEQ ID NO: 10 A combination of second primers having at least a part of the base sequence of the 1741th base sequence or an equivalent base sequence thereof;
<10c> a first primer having at least a partial base sequence of the 1st to 733th base sequence in the base sequence of SEQ ID NO: 10 or an equivalent base sequence thereof, and the 734th to 1163rd positions of the base sequence of SEQ ID NO: 10 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<10d> a first primer having at least a partial base sequence of the 1st to 733th base sequence in the base sequence of SEQ ID NO: 10 or a complementary sequence of the equivalent base sequence; A combination of second primers having at least a part of the base sequence of the 1163rd base sequence or an equivalent base sequence thereof;
<10e> a first primer having at least a partial base sequence of the 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 10 or an equivalent base sequence thereof, and the 1164th to 1741st positions in the base sequence of SEQ ID NO: 10 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<10f> a first primer having at least a partial base sequence of the 734th to 1163rd base sequences in the base sequence of SEQ ID NO: 10 or a complementary sequence of the equivalent base sequence; A combination of second primers having at least a part of the base sequence of the 1741th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (10) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<10a ′> At least a part of the 1-733th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 10 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1164 to 1741 in the base sequence of SEQ ID NO: 10 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<10b ′> At least a part of the 1-733th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 10 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1164th to 1741th base sequences in the base sequence of SEQ ID NO: 10 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<10c ′> At least a part of the 1-733th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 10 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 734 to 1163th base sequence in the base sequence of SEQ ID NO: 10 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<10d ′> At least a part of the 1-733th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 10 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 10 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<10e ′> At least part of the 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 10 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1164 to 1741 in the base sequence of SEQ ID NO: 10 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<10f ′> A base sequence of at least a part of the 734th to 1163rd base sequences in the base sequence of SEQ ID NO: 10 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1164th to 1741th base sequences in the base sequence of SEQ ID NO: 10 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <10a> to <10f> and <10a ′> to <10f ′>, “the 1st to 733th base sequence in the base sequence of SEQ ID NO: 10” is “the 1st to 733th base in the base sequence of SEQ ID NO: 70” In the “sequence”, “the 1164th to 1741th base sequence in the base sequence of SEQ ID NO: 10” may be replaced with “the 734th to 1308th base sequence in the base sequence of SEQ ID NO: 70”, respectively.

  In addition to the four primer combinations <10a '> to <10f'>, two loop primers (LF and LB) may be used in combination.

  The combination of the four primers <10a '> to <10f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <10a '> to <10b'> and any of <10c '> to <10f'>.

<11> Primer set comprising two or more primers capable of detecting the presence or absence of insertion of mPing in polymorphism (11) In the base sequence of SEQ ID NO: 11, the insertion region of mPing is the nucleotide residues 714 to 1143 (FIG. 11). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (11) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (11) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<11a> a first primer having at least a partial base sequence of the 1st to 713th base sequences in the base sequence of SEQ ID NO: 11 or an equivalent base sequence thereof, and 1144th to 1921st positions in the base sequence of SEQ ID NO: 11 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<11b> a first primer having at least a partial base sequence of the 1st to 713th base sequences in the base sequence of SEQ ID NO: 11 or a complementary sequence of the equivalent base sequence, and 1144 to 1144 in the base sequence of SEQ ID NO: 11 A second primer combination having at least a part of the base sequence of the 1921st base sequence or an equivalent base sequence thereof;
<11c> a first primer having at least a partial base sequence of the 1st to 713th base sequence in the base sequence of SEQ ID NO: 11 or an equivalent base sequence thereof, and the 714th to 1143th positions in the base sequence of SEQ ID NO: 11 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<11d> a first primer having at least a partial base sequence of the 1st to 713th base sequences in the base sequence of SEQ ID NO: 11 or a complementary sequence thereof, and 714 to in the base sequence of SEQ ID NO: 11 A combination of second primers having at least a part of the base sequence of the 1143th base sequence or an equivalent base sequence thereof;
<11e> a first primer having at least a partial base sequence of the 714 to 1143th base sequence in the base sequence of SEQ ID NO: 11 or an equivalent base sequence thereof, and the 1141 to 1921st positions of the base sequence of SEQ ID NO: 11 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<11f> a first primer having at least a partial base sequence of the 714th to 1143th base sequence in the base sequence of SEQ ID NO: 11 or a complementary sequence of the equivalent base sequence; A combination of second primers having at least a part of the 1921st base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (11) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<11a ′> The base sequence of at least a part of the 1st to 713th base sequences in the base sequence of SEQ ID NO: 11 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1144th to 1921st base sequences in the base sequence of SEQ ID NO: 11 or a complementary sequence thereof in BIP, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<11b ′> At least a part of the 1st to 713th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 11 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1144th to 1921st base sequences in the base sequence of SEQ ID NO: 11 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<11c ′> At least a part of the 1-713th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 11 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 714 to 1143 in the base sequence of SEQ ID NO: 11 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<11d ′> A base sequence of at least a part of the 1st to 713th base sequences in the base sequence of SEQ ID NO: 11 or a complementary sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the nucleotide sequence of the 714 to 1143th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 11 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<11e ′> At least a part of the base sequence from the 714th to the 1143th base sequence in the base sequence of SEQ ID NO: 11 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1144th to 1921st base sequences in the base sequence of SEQ ID NO: 11 or a complementary sequence thereof in BIP, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<11f ′> At least a part of the nucleotide sequence from the 714th to the 1143th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 11 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1144th to 1921st base sequences in the base sequence of SEQ ID NO: 11 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <11a> to <11f> and <11a ′> to <11f ′>, “the 1st to 713th base sequence in the base sequence of SEQ ID NO: 11” is “the 1st to 713th base in the base sequence of SEQ ID NO: 71” In the “sequence”, “the 1944th to 1921st base sequence in the base sequence of SEQ ID NO: 11” may be replaced with “the 714th to 1488th base sequence in the base sequence of SEQ ID NO: 71”, respectively.

  In addition to the combination of the four primers <11a '> to <11f'>, two loop primers (LF and LB) may be used in combination.

  The combination of the four primers <11a '> to <11f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <11a ′> to <11b ′> and any of <11c ′> to <11f ′>.

<12> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (12);
In the base sequence of SEQ ID NO: 12, the insertion region of mPing is a region consisting of nucleotide residues 769 to 1198 (FIG. 12). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (12) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for the polymorphism (12) mPing, a primer combination comprising the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<12a> a first primer having at least a partial base sequence of the 1st to 768th base sequence in the base sequence of SEQ ID NO: 12 or an equivalent base sequence thereof, and the 1199th to 2053rd positions of the base sequence of SEQ ID NO: 12 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<12b> a first primer having at least a partial base sequence of the 1st to 768th base sequence in the base sequence of SEQ ID NO: 12 or a complementary sequence of the equivalent base sequence, and 1199 in the base sequence of SEQ ID NO: 12 A combination of second primers having at least a part of the base sequence of the 2053rd base sequence or an equivalent base sequence thereof;
<12c> a first primer having at least a partial base sequence of the 1st to 768th base sequence in the base sequence of SEQ ID NO: 12 or an equivalent base sequence thereof, and the 769th to 1198th positions of the base sequence of SEQ ID NO: 12 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<12d> a first primer having at least a partial base sequence of the 1-768th base sequence in the base sequence of SEQ ID NO: 12 or a complementary sequence thereof, and 769- in the base sequence of SEQ ID NO: 12 A combination of second primers having at least a part of the 1198th base sequence or an equivalent base sequence thereof;
<12e> a first primer having at least a partial base sequence of the 769th to 1198th base sequence in the base sequence of SEQ ID NO: 12 or an equivalent base sequence thereof, and the 1199th to 2053rd positions of the base sequence of SEQ ID NO: 12 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<12f> a first primer having at least a partial base sequence of the 769th to 1198th base sequences in the base sequence of SEQ ID NO: 12 or a complementary sequence thereof, and 1199 to 1199 in the base sequence of SEQ ID NO: 12 A combination of second primers having at least part of the base sequence of the 2053rd base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (12) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<12a ′> At least a part of the 1-768th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 12 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1199 to 2053 in the base sequence of SEQ ID NO: 12, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<12b ′> At least a part of the 1st to 768th base sequence in the base sequence of SEQ ID NO: 12 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the base sequence of 1199 to 2053 in the base sequence of SEQ ID NO: 12 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<12c ′> At least a part of the 1-768th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 12 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of positions 769 to 1198 in the base sequence of SEQ ID NO: 12 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<12d ′> A base sequence of at least a part of the 1st to 768th base sequence in the base sequence of SEQ ID NO: 12 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 769 to 1198th base sequence in the base sequence of SEQ ID NO: 12 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<12e ′> At least a part of the 769 to 1198th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 12 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1199 to 2053 in the base sequence of SEQ ID NO: 12, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<12f ′> A base sequence of at least a part of the 769th to 1198th base sequences in the base sequence of SEQ ID NO: 12 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the base sequence of 1199 to 2053 in the base sequence of SEQ ID NO: 12 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <12a> to <12f> and <12a ′> to <12f ′>, “the 1st to 768th base sequence in the base sequence of SEQ ID NO: 12” is “the 1st to 768th base in the base sequence of SEQ ID NO: 72” In the “sequence”, “the 1199 to 2053rd base sequence in the base sequence of SEQ ID NO: 12” may be replaced with “the 769th to 1620th base sequence in the base sequence of SEQ ID NO: 72”.

  In addition to the four primer combinations <12a ′> to <12f ′>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <12a ′> to <12f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <12a ′> to <12b ′> and any of <12c ′> to <12f ′>.

<13> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (13) In the base sequence of SEQ ID NO: 13, the insertion region of mPing is the 733th to 1162nd nucleotide residues (FIG. 13). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (13) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of polymorphism (13), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<13a> a first primer having at least a part of the base sequence of 1 to 732 in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof, and 1163 to 1807 of the base sequence of SEQ ID NO: 13 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<13b> a first primer having at least a partial base sequence of the 1-732th base sequence in the base sequence of SEQ ID NO: 13 or a complementary sequence thereof, and 1163 in the base sequence of SEQ ID NO: 13 A combination of second primers having at least a part of the base sequence of the 1807th base sequence or an equivalent base sequence thereof;
<13c> a first primer having at least a part of the 1-732th base sequence in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof, and the 733-116th positions in the base sequence of SEQ ID NO: 13 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<13d> a first primer having at least a partial base sequence of the 1st to 732th base sequence in the base sequence of SEQ ID NO: 13 or a complementary sequence thereof, and 733- 3 in the base sequence of SEQ ID NO: 13 A combination of second primers having at least part of the 1162nd base sequence or an equivalent base sequence thereof;
<13e> a first primer having at least a part of the base sequence of 733 to 1162 in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof, and 1163 to 1807 of the base sequence of SEQ ID NO: 3 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<13f> a first primer having at least a part of the base sequence of the 733 to 1162 base sequences in the base sequence of SEQ ID NO: 13 or a complementary sequence thereof, and 1163 in the base sequence of SEQ ID NO: 13 The 2nd primer combination which has at least one part base sequence of the 1807th base sequence, or its equivalent base sequence.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (13) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<13a ′> A base sequence of at least a part of the base sequence 1 to 732 in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1163 to 1807 in the base sequence of SEQ ID NO: 13 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<13b ′> At least a part of the 1-732th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 13 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1163rd to 1807th base sequences in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<13c ′> At least a part of the 1-732 base sequence in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably The first primer having (as F2 region in FIP), and at least a part of the base sequence of 733 to 1162 in the base sequence of SEQ ID NO: 13 or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<13d ′> A base sequence of at least a part of the 1st to 732rd base sequence in the base sequence of SEQ ID NO: 13 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 733 to 1162 base sequences in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<13e ′> At least a part of the base sequence of the 733 to 1162 base sequence in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region ( A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1163 to 1807 in the base sequence of SEQ ID NO: 13 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<13f ′> A base sequence of at least a part of the 733th to 1162nd base sequences in the base sequence of SEQ ID NO: 13 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1163rd to 1807th base sequences in the base sequence of SEQ ID NO: 13 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <13a> to <13f> and <13a ′> to <13f ′>, “the 1st to 732th base sequence in the base sequence of SEQ ID NO: 13” is “the 1st to 732th base in the base sequence of SEQ ID NO: 73” In the “sequence”, “the 1163rd to 1807th base sequence in the base sequence of SEQ ID NO: 13” may be replaced with “the 733th to 1374th base sequence in the base sequence of SEQ ID NO: 73”, respectively.

  In addition to the four primer combinations <13a ′> to <13f ′>, two loop primers (LF and LB) may be used in combination.

  The combinations of the four primers <13a ′> to <13f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <13a ′> to <13b ′> and any of <13c ′> to <13f ′>.

<14> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (14);
In the base sequence of SEQ ID NO: 14, the mPing insertion region is a region consisting of nucleotide residues 532 to 961 (FIG. 14). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (14) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (14) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<14a> a first primer having at least a part of the base sequence of 1 to 531 in the base sequence of SEQ ID NO: 14 or an equivalent base sequence thereof, and the 962 to 1716th positions of the base sequence of SEQ ID NO: 14 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<14b> a first primer having at least a partial base sequence of the first to 531st base sequences in the base sequence of SEQ ID NO: 14 or a complementary sequence of the equivalent base sequence, and 962 in the base sequence of SEQ ID NO: 14 A combination of second primers having at least a part of the base sequence of the 1716th base sequence or an equivalent base sequence thereof;
<14c> a first primer having at least a part of a base sequence of 1 to 531 in the base sequence of SEQ ID NO: 14 or an equivalent base sequence thereof, and 532 to 961 in the base sequence of SEQ ID NO: 14 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<14d> a first primer having at least a partial base sequence of the first to 531st base sequences in the base sequence of SEQ ID NO: 14 or a complementary sequence of the equivalent base sequence, and 532- in the base sequence of SEQ ID NO: 14 A combination of second primers having at least part of the 961st base sequence or an equivalent base sequence thereof;
<14e> a first primer having at least a part of the base sequence of the 532 to 961 base sequence in the base sequence of SEQ ID NO: 14 or an equivalent base sequence thereof, and the 962 to 1716 th base sequence of the base sequence of SEQ ID NO: 14 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<14f> a first primer having at least a partial base sequence of the 532-961st base sequence in the base sequence of SEQ ID NO: 14 or a complementary sequence of the equivalent base sequence, and 962- in the base sequence of SEQ ID NO: 14 A combination of second primers having at least a part of the base sequence of the 1716th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (14) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<14a ′> A base sequence of at least a part of the first to 531st base sequences in the base sequence of SEQ ID NO: 14 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the 962 to 1716th base sequence in the base sequence of SEQ ID NO: 14 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<14b ′> A base sequence of at least a part of the first to 531st base sequences in the base sequence of SEQ ID NO: 14 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 962 to 1716th base sequence in the base sequence of SEQ ID NO: 14 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<14c ′> At least a part of the base sequence of the first to 531st base sequences in the base sequence of SEQ ID NO: 14 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 532 to 961 in the base sequence of SEQ ID NO: 14 or a complementary sequence of the equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<14d '> A base sequence of at least a part of the 1st to 531st base sequences in the base sequence of SEQ ID NO: 14 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 532 to 961 base sequences in the base sequence of SEQ ID NO: 14 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<14e ′> At least a part of the 532-961st nucleotide sequence in the nucleotide sequence of SEQ ID NO: 14 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the 962 to 1716th base sequence in the base sequence of SEQ ID NO: 14 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<14f ′> A base sequence of at least a part of the 532nd to 961st base sequences in the base sequence of SEQ ID NO: 14 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 962 to 1716th base sequence in the base sequence of SEQ ID NO: 14 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c region or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <14a> to <14f> and <14a ′> to <14f ′>, “the 1st to 531st base sequence in the base sequence of SEQ ID NO: 14” is “the 1st to 531st base in the base sequence of SEQ ID NO: 74” In the “sequence”, “the 962 to 1716th base sequence in the base sequence of SEQ ID NO: 14” may be replaced with “the 532 to 1283th base sequence in the base sequence of SEQ ID NO: 74”, respectively.

  In addition to the four primer combinations <14a '> to <14f'>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <14a ′> to <14f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <14a '> to <14b'> and any of <14c '> to <14f'>.

<15> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (15) In the base sequence of SEQ ID NO: 15, the insertion region of mPing is the 723 to 1152nd nucleotide residues (FIG. 15). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (15) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of polymorphism (15), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<15a> a first primer having at least a part of the base sequence of 1 to 722 in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof, and the 1153 to 1854th of the base sequence of SEQ ID NO: 15 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<15b> a first primer having at least a partial base sequence of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 15 or a complementary sequence thereof, and 1153 in the base sequence of SEQ ID NO: 15 A combination of second primers having at least a part of the base sequence of the 1854th base sequence or an equivalent base sequence thereof;
<15c> a first primer having at least a part of the base sequence of 1 to 722 in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof, and the 723 to 1152 bases of the base sequence of SEQ ID NO: 15 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<15d> a first primer having at least a partial base sequence of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 15 or a complementary sequence thereof, and 723 in the base sequence of SEQ ID NO: 15 A combination of second primers having at least a part of the 1152nd base sequence or an equivalent base sequence thereof;
<15e> a first primer having at least a part of the base sequence of 723 to 1152 in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof, and the 1153 to 1854th of the base sequence of SEQ ID NO: 15 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<15f> a first primer having at least a partial base sequence of the 723 to 1152 base sequences in the base sequence of SEQ ID NO: 15 or a complementary sequence of the equivalent base sequence, and 1153 in the base sequence of SEQ ID NO: 15 A combination of second primers having at least a part of the base sequence of the 1854th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (15) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<15a ′> At least a part of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1153 to 1854 in the base sequence of SEQ ID NO: 15 or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<15b ′> At least a part of the 1st to 722nd nucleotide sequence in the nucleotide sequence of SEQ ID NO: 15 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of 1153 to 1854th base sequence in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<15c ′> The base sequence of at least a part of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 723 to 1152 in the base sequence of SEQ ID NO: 4 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<15d '> A base sequence of at least a part of the 1st to 722nd base sequences in the base sequence of SEQ ID NO: 15 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 723 to 1152 base sequences in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<15e ′> At least a part of the base sequence of the 723 to 1152 base sequence in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1153 to 1854 in the base sequence of SEQ ID NO: 15 or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<15f ′> A base sequence of at least a part of the 723 to 1152 base sequences in the base sequence of SEQ ID NO: 15 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of 1153 to 1854th base sequence in the base sequence of SEQ ID NO: 15 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <15a> to <15f> and <15a ′> to <15f ′>, “the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 15” is “the 1st to 722th base in the base sequence of SEQ ID NO: 75” In “Sequence”, “the 1153 to 1854th base sequence in the base sequence of SEQ ID NO: 15” may be replaced with “the 723 to 1421st base sequence in the base sequence of SEQ ID NO: 75”, respectively.

  In addition to the combination of the four primers <15a ′> to <15f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <15a '> to <15f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <15a '> to <15b'> and any of <15c '> to <15f'>.

<16> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (16);
In the base sequence of SEQ ID NO: 16, the insertion region of mPing is a region consisting of nucleotide residues 754 to 1183 (FIG. 16). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (16) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (16) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<16a> a first primer having at least a partial base sequence of the 1st to 753rd base sequences in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof, and the 1184th to 1932th bases of the base sequence of SEQ ID NO: 16 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<16b> a first primer having at least a partial base sequence of the 1st to 753rd base sequences in the base sequence of SEQ ID NO: 16 or a complementary sequence of the equivalent base sequence, and 1184-in the base sequence of SEQ ID NO: 16 A second primer combination having at least a part of the base sequence of the 1932nd base sequence or an equivalent base sequence thereof;
<16c> a first primer having at least a part of the 1st to 753rd base sequence in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof, and the 754th to 1183th positions in the base sequence of SEQ ID NO: 16 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<16d> a first primer having at least a partial base sequence of the 1st to 753rd base sequences in the base sequence of SEQ ID NO: 16 or a complementary sequence thereof, and 754 to A combination of second primers having at least a part of the base sequence of the 1183rd base sequence or an equivalent base sequence thereof;
<16e> a first primer having at least a partial base sequence of the 754 to 1183th base sequence in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof, and the 1184th to 1932th bases of the base sequence of SEQ ID NO: 16 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<16f> a first primer having at least a part of the base sequence of the 754 to 1183th base sequence in the base sequence of SEQ ID NO: 16 or a complementary sequence of the equivalent base sequence, and 1184 of the base sequence of SEQ ID NO: 16 The combination of the 2nd primer which has at least one part base sequence of the 1932nd base sequence, or its equivalent base sequence.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (16) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<16a ′> At least a part of the base sequence of the 1st to 753rd base sequences in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence from 1184 to 1932 in the base sequence of SEQ ID NO: 16 or a complementary sequence thereof in BIP, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<16b ′> At least a part of the 1st to 753rd base sequences in the base sequence of SEQ ID NO: 16 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1184th to 1932th base sequences in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<16c ′> At least a part of the base sequence of the 1st to 753rd base sequences in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 754 to 1183 in the base sequence of SEQ ID NO: 16 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<16d ′> At least a part of the base sequence of the 1st to 753rd base sequences in the base sequence of SEQ ID NO: 16 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 754 to 1183th base sequence in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<16e ′> A base sequence of at least a part of the 754th to 1183rd base sequences in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence from 1184 to 1932 in the base sequence of SEQ ID NO: 16 or a complementary sequence thereof in BIP, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<16f ′> At least a part of the nucleotide sequence from the 754th to 1183th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 16 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1184th to 1932th base sequences in the base sequence of SEQ ID NO: 16 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <16a> to <16f> and <16a ′> to <16f ′>, “the 1st to 753rd base sequence in the base sequence of SEQ ID NO: 16” is “the 1st to 753rd base in the base sequence of SEQ ID NO: 76” In the “sequence”, “the 1184th to 1932nd base sequence in the base sequence of SEQ ID NO: 16” may be replaced with “the 754th to 1499th base sequence in the base sequence of SEQ ID NO: 76”.

  In addition to the four primer combinations <16a ′> to <16f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <16a '> to <16f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <16a ′> to <16b ′> and any of <16c ′> to <16f ′>.

<17> Primer set comprising two or more primers capable of detecting the presence or absence of insertion of mPing in the polymorphism (17) In the nucleotide sequence of SEQ ID NO: 17, the insertion region of mPing is the nucleotide residues 728 to 1157 (FIG. 17). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (17) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (17) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such primer combinations (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<17a> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 17 or an equivalent base sequence thereof, and the 1158th to 1813th positions of the base sequence of SEQ ID NO: 17 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<17b> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 17 or a complementary sequence thereof, and 1158 to 1158 in the base sequence of SEQ ID NO: 17 A combination of second primers having at least part of the base sequence of the 1813th base sequence or an equivalent base sequence thereof;
<17c> a first primer having at least a part of the 1-727th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 17 or an equivalent nucleotide sequence thereof, and the 728-1157th nucleotide in the nucleotide sequence of SEQ ID NO: 17 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<17d> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 17 or a complementary sequence of the equivalent base sequence, and 728 in the base sequence of SEQ ID NO: 17 A combination of second primers having at least a part of the base sequence of the 1157th base sequence or an equivalent base sequence thereof;
<17e> a first primer having at least a partial base sequence of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 17 or an equivalent base sequence thereof, and the 1158th to 1813th bases of the base sequence of SEQ ID NO: 17 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<17f> a first primer having at least a partial base sequence of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 17 or a complementary sequence of the equivalent base sequence, and 1158-in the base sequence of SEQ ID NO: 17 A combination of second primers having at least a part of the base sequence of the 1813th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (17) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<17a ′> At least a part of the 1-727th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 17 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1158 to 1813 in the base sequence of SEQ ID NO: 17 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<17b ′> A base sequence of at least a part of the 1st to 727th base sequences in the base sequence of SEQ ID NO: 17 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1158th to 1813th base sequences in the base sequence of SEQ ID NO: 17 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<17c ′> At least a part of the 1-727th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 17 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the nucleotide sequence of 728 to 1157th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 17 or a complementary sequence thereof in BIP, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<17d ′> A base sequence of at least a part of the 1st to 727th base sequences in the base sequence of SEQ ID NO: 17 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the nucleotide sequence of the 728 to 1157th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 17 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<17e ′> At least a part of the base sequence from 728 to 1157 in the base sequence of SEQ ID NO: 17 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1158 to 1813 in the base sequence of SEQ ID NO: 17 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<17f ′> A base sequence of at least a part of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 17 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1158th to 1813th base sequences in the base sequence of SEQ ID NO: 17 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <17a> to <17f> and <17a ′> to <17f ′>, “the 1st to 727th base sequence in the base sequence of SEQ ID NO: 17” is “the 1st to 727th base in the base sequence of SEQ ID NO: 77” In the “sequence”, “the 1158th to 1813th base sequence in the base sequence of SEQ ID NO: 17” may be replaced with “the 728th to 1380th base sequence in the base sequence of SEQ ID NO: 77”.

  In addition to the four primer combinations <17a '> to <17f'>, two loop primers (LF and LB) may be used in combination.

  The four primer combinations <17a ′> to <17f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <17a ′> to <17b ′> and any of <17c ′> to <17f ′>.

<18> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (18);
In the base sequence of SEQ ID NO: 18, the mPing insertion region is a region consisting of nucleotide residues 717 to 1146 (FIG. 18). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (18) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (18) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<18a> a first primer having at least a partial base sequence of the 1st to 716th base sequence in the base sequence of SEQ ID NO: 18 or an equivalent base sequence thereof, and the 1147th to 1859th positions of the base sequence of SEQ ID NO: 18 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<18b> a first primer having at least a partial base sequence of the 1st to 716th base sequences in the base sequence of SEQ ID NO: 18 or a complementary sequence thereof, and 1147 to 1 in the base sequence of SEQ ID NO: 18 A combination of second primers having at least a part of the base sequence of the 1859th base sequence or an equivalent base sequence thereof;
<18c> a first primer having at least a partial base sequence of the 1st to 716th base sequence in the base sequence of SEQ ID NO: 18 or an equivalent base sequence thereof, and the 717th to 1146th positions of the base sequence of SEQ ID NO: 18 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<18d> a first primer having at least a partial base sequence of the 1st to 716th base sequences in the base sequence of SEQ ID NO: 18 or a complementary sequence thereof, and 717 to in the base sequence of SEQ ID NO: 18 A combination of second primers having at least a part of the 1146th base sequence or an equivalent base sequence thereof;
<18e> a first primer having at least a part of a base sequence of 717 to 1146th in the base sequence of SEQ ID NO: 18 or an equivalent base sequence thereof, and 1147 to 1859th of the base sequence of SEQ ID NO: 18 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<18f> a first primer having at least a partial base sequence of the 717 to 1146th base sequence in the base sequence of SEQ ID NO: 18 or a complementary sequence thereof, and 1147 to 1 in the base sequence of SEQ ID NO: 18 A combination of second primers having at least a part of the base sequence of the 1859th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (18) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<18a ′> At least a part of the 1-716th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 18 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence from 1147 to 1859 in the base sequence of SEQ ID NO: 18, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<18b ′> At least a part of the 1st to 716th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 18 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1147th to 1859th base sequences in the base sequence of SEQ ID NO: 18 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<18c ′> At least a part of the 1-716th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 18 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the nucleotide sequence of 717 to 1146th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 18 or a complementary sequence of the equivalent nucleotide sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<18d ′> A base sequence of at least a part of the 1st to 716th base sequences in the base sequence of SEQ ID NO: 18 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the nucleotide sequence of the 717 to 1146th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 18 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<18e ′> At least a part of the base sequence from the 717th to 1146th base sequence in the base sequence of SEQ ID NO: 18 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence from 1147 to 1859 in the base sequence of SEQ ID NO: 18, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<18f ′> A base sequence of at least a part of the 717th to 1146th base sequence in the base sequence of SEQ ID NO: 18 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1147th to 1859th base sequences in the base sequence of SEQ ID NO: 18 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <18a> to <18f> and <18a ′> to <18f ′>, “the 1st to 716th base sequence in the base sequence of SEQ ID NO: 18” is “the 1st to 716th base in the base sequence of SEQ ID NO: 78” In the “sequence”, “the 1471st to 1859th base sequence in the base sequence of SEQ ID NO: 18” may be replaced with “the 717th to 1426th base sequence in the base sequence of SEQ ID NO: 78”.

  In addition to the combination of the four primers <18a ′> to <18f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <18a ′> to <18f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <18a '> to <18b'> and any of <18c '> to <18f'>.

<19> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (19) In the base sequence of SEQ ID NO: 19, the insertion region of mPing is the 633 to 1062nd nucleotide residue (FIG. 19). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (19) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (19) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<19a> a first primer having at least a part of a base sequence of 1 to 632 in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof, and 1063 to 1876 in the base sequence of SEQ ID NO: 19 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<19b> a first primer having at least a partial base sequence of the 1st to 632rd base sequence in the base sequence of SEQ ID NO: 19 or a complementary sequence thereof, and 1063 in the base sequence of SEQ ID NO: 19 A combination of second primers having at least a part of the base sequence of the 1876th base sequence or an equivalent base sequence thereof;
<19c> a first primer having at least a part of a base sequence of 1 to 632 in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof, and 633 to 1062 in the base sequence of SEQ ID NO: 19 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<19d> a first primer having at least a part of the 1-632 base sequence in the base sequence of SEQ ID NO: 19 or a complementary sequence equivalent thereto, and 633 in the base sequence of SEQ ID NO: 19 A combination of second primers having at least part of the 1062nd base sequence or an equivalent base sequence thereof;
<19e> a first primer having at least a part of a base sequence of the 633 to 1062 base sequence in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof, and a 1063 to 1876 th base sequence of the base sequence of SEQ ID NO: 19 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<19f> A first primer having at least a part of the base sequence of the 633 to 1062 base sequence in the base sequence of SEQ ID NO: 19 or a complementary sequence equivalent thereto, and 1063 in the base sequence of SEQ ID NO: 19. A combination of second primers having at least part of the base sequence of the 1876th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (19) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<19a ′> At least a part of the 1-632 base sequence in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1063 to 1876 in the base sequence of SEQ ID NO: 19, or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<19b ′> At least a part of the 1-632 base sequence in the base sequence of SEQ ID NO: 19 or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1063 to 1876th base sequence in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<19c ′> At least a part of the 1-632 base sequence in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 633 to 1062 in the base sequence of SEQ ID NO: 4 or a complementary sequence of the equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<19d ′> At least a part of the 1-632 base sequence in the base sequence of SEQ ID NO: 19 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 633 to 1062 base sequences in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<19e ′> At least a part of the base sequence of the 633 to 1062 base sequence in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1063 to 1876 in the base sequence of SEQ ID NO: 19, or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<19f ′> At least a part of the base sequence of the 633 to 1062 base sequence in the base sequence of SEQ ID NO: 19 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1063 to 1876th base sequence in the base sequence of SEQ ID NO: 19 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <19a> to <19f> and <19a ′> to <19f ′>, “the 1st to 632nd base sequence in the base sequence of SEQ ID NO: 19” is “the 1st to 632th base in the base sequence of SEQ ID NO: 79” In “Sequence”, “the 1063 to 1876th base sequence in the base sequence of SEQ ID NO: 19” may be replaced with “the 633 to 1443th base sequence in the base sequence of SEQ ID NO: 79”.

  In addition to the four primer combinations <19a '> to <19f'>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <19a '> to <19f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <19a '> to <19b'> and any of <19c '> to <19f'>.

<20> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (20);
In the base sequence of SEQ ID NO: 20, the mPing insertion region is a region consisting of nucleotide residues 731 to 1160 (FIG. 20). Therefore, as two or more primers for detecting the presence or absence of mPing insertion of the polymorphism (20), a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (20) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations). PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<20a> a first primer having at least a partial base sequence of the 1st to 730th base sequence in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof, and the 1161st to 1871st positions of the base sequence of SEQ ID NO: 20 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<20b> a first primer having at least a partial base sequence of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 20 or a complementary sequence of the equivalent base sequence, and 1161 in the base sequence of SEQ ID NO: 20 A combination of second primers having at least a part of the base sequence of the 1871st base sequence or an equivalent base sequence thereof;
<20c> a first primer having at least a partial base sequence of the 1st to 730th base sequence in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof, and the 731st to 1160th base sequence of the base sequence of SEQ ID NO: 20 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<20d> a first primer having at least a partial base sequence of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 20 or a complementary sequence of the equivalent base sequence, and 731-1 in the base sequence of SEQ ID NO: 20 A combination of second primers having at least a part of the base sequence of the 1160th base sequence or an equivalent base sequence thereof;
<20e> a first primer having at least a partial base sequence of the 731 to 1160th base sequence in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof, and the 1161 to 1871st positions of the base sequence of SEQ ID NO: 20 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<20f> a first primer having at least a partial base sequence of the 731st to 1160th base sequences in the base sequence of SEQ ID NO: 20 or a complementary sequence of the equivalent base sequence, and 1161 in the base sequence of SEQ ID NO: 20 A combination of second primers having at least a part of the base sequence of the 1871st base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (20) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<20a ′> A base sequence of at least a part of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region A first primer having (as F2 region in FIP), and at least a part of the base sequence of positions 1161-1187 in the base sequence of SEQ ID NO: 20 or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<20b ′> A base sequence of at least a part of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 20 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1161 to 1871st base sequence in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<20c ′> At least a part of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 731 to 1160 in the base sequence of SEQ ID NO: 20, or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<20d ′> A base sequence of at least a part of the 1st to 730th base sequences in the base sequence of SEQ ID NO: 20 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 731st to 1160th base sequences in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<20e ′> At least a part of the 731 to 1160th base sequence in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of positions 1161-1187 in the base sequence of SEQ ID NO: 20 or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<20f ′> A base sequence of at least a part of the 731st to 1160th base sequences in the base sequence of SEQ ID NO: 20 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1161 to 1871st base sequence in the base sequence of SEQ ID NO: 20 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <20a> to <20f> and <20a ′> to <20f ′>, “the 1st to 730th base sequence in the base sequence of SEQ ID NO: 20” is “the 1st to 730th base in the base sequence of SEQ ID NO: 80” In the “sequence”, the “1161 to 1871st base sequence in the base sequence of SEQ ID NO: 20” may be replaced with “the 731 to 1438th base sequence in the base sequence of SEQ ID NO: 80”, respectively.

  In addition to the combination of the four primers <20a ′> to <20f ′>, two loop primers (LF and LB) may be used in combination.

  The combinations of the four primers <20a '> to <20f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <20a '> to <20b'> and any of <20c '> to <20f'>.

<21> Primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (21) In the base sequence of SEQ ID NO: 21, the insertion region of mPing is the nucleotide residues from 687 to 1116 (FIG. 21). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (21) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of the polymorphism (21), a combination of primers composed of the following primer pairs (eg, primers for PCR) and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<21a> a first primer having at least a partial base sequence of the 1st to 686th base sequence in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof, and the 1117th to 1928th positions of the base sequence of SEQ ID NO: 21 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<21b> a first primer having at least a partial base sequence of the 1st to 686th base sequence in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof, and 1117 to 1 in the base sequence of SEQ ID NO: 21 A second primer combination having at least a part of the base sequence of the 1928th base sequence or an equivalent base sequence thereof;
<21c> a first primer having at least a part of the 1st to 686th base sequence in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof, and the 687th to 1116th positions in the base sequence of SEQ ID NO: 21 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<21d> a first primer having at least a partial base sequence of the 1st to 686th base sequence in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof, and 687 in the base sequence of SEQ ID NO: 21 A combination of second primers having at least a part of the base sequence of the 1116th base sequence or an equivalent base sequence thereof;
<21e> a first primer having at least a part of the base sequence of the 687 to 1116th base sequence in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof, and the 1117th to 1928th positions of the base sequence of SEQ ID NO: 21 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<21f> a first primer having at least a part of the base sequence of the 687 to 1116th base sequence in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof, and 1117 to the base sequence of SEQ ID NO: 21 A combination of second primers having at least a part of the 1928th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (21) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<21a ′> At least a part of the 1st to 686th base sequences in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1117th to 1928th base sequences in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<21b ′> A base sequence of at least a part of the 1st to 686th base sequences in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1117th to 1928th base sequences in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<21c ′> At least a part of the 1-686th base sequence in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably The first primer having (as F2 region in FIP), and at least a part of the base sequence of the 687 to 1116th base sequence in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<21d ′> At least a portion of the 1st to 686th base sequence in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 687 to 1116th base sequence in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<21e '> A base sequence of at least a part of the 687th to 1116th base sequences in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1117th to 1928th base sequences in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<21f '> A base sequence of at least a part of the 687th to 1116th base sequences in the base sequence of SEQ ID NO: 21 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1117th to 1928th base sequences in the base sequence of SEQ ID NO: 21 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <21a> to <21f> and <21a ′> to <21f ′>, “the 1st to 686th base sequence in the base sequence of SEQ ID NO: 21” is “the 1st to 686th base in the base sequence of SEQ ID NO: 81” In the “sequence”, “the 1117th to 1928th base sequence in the base sequence of SEQ ID NO: 21” may be replaced with “the 687th to 1495th base sequence in the base sequence of SEQ ID NO: 81”, respectively.

  In addition to the four primer combinations <21a '> to <21f'>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <21a '> to <21f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <21a '> to <21b'> and any of <21c '> to <21f'>.

<22> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (22);
In the base sequence of SEQ ID NO: 22, the mPing insertion region is a region consisting of nucleotide residues 728 to 1157 (FIG. 22). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (22) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of polymorphism (22), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<22a> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 22 or an equivalent base sequence thereof, and the 1158th to 1917th positions of the base sequence of SEQ ID NO: 22 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<22b> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 22 or a complementary sequence thereof, and 1158 to 1 in the base sequence of SEQ ID NO: 22 A second primer combination having at least a part of the base sequence of the 1917th base sequence or an equivalent base sequence thereof;
<22c> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 22 or an equivalent base sequence thereof, and the 728th to 1157th positions in the base sequence of SEQ ID NO: 22 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<22d> a first primer having at least a partial base sequence of the 1st to 727th base sequences in the base sequence of SEQ ID NO: 22 or a complementary sequence of the equivalent base sequence, and 728 in the base sequence of SEQ ID NO: 22 A combination of second primers having at least a part of the base sequence of the 1157th base sequence or an equivalent base sequence thereof;
<22e> a first primer having at least a partial base sequence of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 22 or an equivalent base sequence thereof, and the 1158th to 1917th positions in the base sequence of SEQ ID NO: 22 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<22f> a first primer having at least a partial base sequence of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 22 or a complementary sequence of the equivalent base sequence, and 1158-in the base sequence of SEQ ID NO: 22 The combination of the 2nd primer which has at least one part base sequence of the 1917th base sequence, or its equivalent base sequence.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (22) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<22a ′> A base sequence of at least a part of the 1st to 727th base sequences in the base sequence of SEQ ID NO: 22 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1158th to 1917th base sequences in the base sequence of SEQ ID NO: 22 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<22b ′> A base sequence of at least a part of the 1st to 727th base sequences in the base sequence of SEQ ID NO: 22 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1158th to 1917th base sequences in the base sequence of SEQ ID NO: 22 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<22c ′> At least a part of the 1-727th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 22 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the nucleotide sequence of 728 to 1157th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 22 or a complementary sequence of the equivalent nucleotide sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<22d ′> At least a part of the 1st to 727th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 22 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the nucleotide sequence of the 728 to 1157th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 22 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<22e '> The base sequence of at least a part of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 22 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1158th to 1917th base sequences in the base sequence of SEQ ID NO: 22 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<22f ′> At least a part of the nucleotide sequence of the 728 to 1157th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 22 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1158th to 1917th base sequences in the base sequence of SEQ ID NO: 22 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <22a> to <22f> and <22a ′> to <22f ′>, “the 1st to 727th base sequence in the base sequence of SEQ ID NO: 22” is “the 1st to 727th base in the base sequence of SEQ ID NO: 82” In “Sequence”, “1158th to 1917th base sequence in the base sequence of SEQ ID NO: 22” may be replaced with “728th to 1484th base sequence in the base sequence of SEQ ID NO: 82”.

  In addition to the four primer combinations <22a ′> to <22f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <22a '> to <22f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <22a '> to <22b'> and any of <22c '> to <22f'>.

<23> Primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (23) In the base sequence of SEQ ID NO: 23, the insertion region of mPing is the nucleotide residues 804 to 1233 (FIG. 23). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (23) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of polymorphism (23), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<23a> a first primer having at least a part of the base sequence of 1 to 803 in the base sequence of SEQ ID NO: 23 or an equivalent base sequence thereof, and the 1234th to 1940th positions in the base sequence of SEQ ID NO: 23 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<23b> a first primer having at least a partial base sequence of the 1st to 803th base sequences in the base sequence of SEQ ID NO: 23 or a complementary sequence thereof, and 1234 in the base sequence of SEQ ID NO: 23 A second primer combination having at least a part of the 1940th base sequence or an equivalent base sequence thereof;
<23c> a first primer having at least a partial base sequence of the 1st to 803th base sequences in the base sequence of SEQ ID NO: 23 or an equivalent base sequence thereof, and the 804 to 1233th positions of the base sequence of SEQ ID NO: 23 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<23d> a first primer having at least a partial base sequence of the 1st to 803th base sequences in the base sequence of SEQ ID NO: 23 or a complementary sequence thereof, and 804 to in the base sequence of SEQ ID NO: 23 A second primer combination having at least a part of the base sequence of the 1233rd base sequence or an equivalent base sequence thereof;
<23e> a first primer having at least a partial base sequence of the 804 to 1233th base sequence in the base sequence of SEQ ID NO: 23 or an equivalent base sequence thereof, and the 1234 to 1940th positions in the base sequence of SEQ ID NO: 23 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<23f> a first primer having at least a partial base sequence of the 804 to 1233th base sequence in the base sequence of SEQ ID NO: 23 or a complementary sequence of the equivalent base sequence, and 1234 in the base sequence of SEQ ID NO: 23 A combination of second primers having at least a part of the 1940th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (23) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<23a ′> At least a part of the base sequence of the 1st to 803th base sequence in the base sequence of SEQ ID NO: 23 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1234 to 1940 in the base sequence of SEQ ID NO: 23, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<23b ′> A base sequence of at least a part of the 1st to 803th base sequences in the base sequence of SEQ ID NO: 23 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 1234 to 1940th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 23 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<23c ′> The base sequence of at least a part of the 1st to 803th base sequences in the base sequence of SEQ ID NO: 23 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 804 to 1233 in the base sequence of SEQ ID NO: 23, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<23d '> A base sequence of at least a part of the 1st to 803th base sequences in the base sequence of SEQ ID NO: 23 or a complementary sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 804 to 1233th base sequence in the base sequence of SEQ ID NO: 23, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<23e ′> At least a part of the base sequence from the 804th to the 1233th base sequence in the base sequence of SEQ ID NO: 23 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1234 to 1940 in the base sequence of SEQ ID NO: 23, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<23f ′> A base sequence of at least a part of the 804 to 1233th base sequence in the base sequence of SEQ ID NO: 23 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 1234 to 1940th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 23 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <23a> to <23f> and <23a ′> to <23f ′>, “the 1st to 803th base sequence in the base sequence of SEQ ID NO: 23” is “the 1st to 803th base in the base sequence of SEQ ID NO: 83” In the “sequence”, “the 1234th to 1940th base sequence in the base sequence of SEQ ID NO: 23” may be replaced with “the 804th to 1507th base sequence in the base sequence of SEQ ID NO: 83”, respectively.

  In addition to the four primer combinations <23a '> to <23f'>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <23a '> to <23f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <23a '> to <23b'> and any of <23c '> to <23f'>.

<24> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (24);
In the base sequence of SEQ ID NO: 24, the mPing insertion region is a region consisting of nucleotide residues 695 to 1124 (FIG. 24). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (24) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of polymorphism (24), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<24a> a first primer having at least a partial base sequence of the 1st to 694th base sequences in the base sequence of SEQ ID NO: 24 or an equivalent base sequence thereof, and the 1125th to 1801th bases of the base sequence of SEQ ID NO: 24 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<24b> a first primer having at least a partial base sequence of the 1st to 694th base sequences in the base sequence of SEQ ID NO: 24 or a complementary sequence of the equivalent base sequence, and 1125 in the base sequence of SEQ ID NO: 24 A combination of second primers having at least a part of the base sequence of the 1801st base sequence or an equivalent base sequence thereof;
<24c> a first primer having at least a part of the 1st to 694th base sequence in the base sequence of SEQ ID NO: 24 or an equivalent base sequence thereof, and the 695th to 1124th positions of the base sequence of SEQ ID NO: 24 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<24d> a first primer having at least a partial base sequence of the 1st to 694th base sequences in the base sequence of SEQ ID NO: 24 or a complementary sequence of the equivalent base sequence, and 695 in the base sequence of SEQ ID NO: 24 A combination of second primers having at least a part of the base sequence of the 1124th base sequence or an equivalent base sequence thereof;
<24e> a first primer having at least a part of a base sequence of 695 to 1124th in the base sequence of SEQ ID NO: 24 or an equivalent base sequence thereof, and 1125 to 1801th in a base sequence of SEQ ID NO: 24 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<24f> a first primer having at least a partial base sequence of the 695th to 1124th base sequences in the base sequence of SEQ ID NO: 24 or a complementary sequence of the equivalent base sequence, and 1125 in the base sequence of SEQ ID NO: 24 A combination of second primers having at least a part of the base sequence of the 1801st base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (24) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<24a ′> At least a part of the 1st to 694th base sequences in the base sequence of SEQ ID NO: 24 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1125 to 1801 in the base sequence of SEQ ID NO: 24, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<24b ′> A base sequence of at least a portion of the 1st to 694th base sequences in the base sequence of SEQ ID NO: 24, or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 1125 to 1801 in the base sequence of SEQ ID NO: 24, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<24c ′> At least a part of the 1st to 694th base sequences in the base sequence of SEQ ID NO: 24 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 695 to 1124 in the base sequence of SEQ ID NO: 24 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<24d '> A base sequence of at least a part of the 1st to 694th base sequences in the base sequence of SEQ ID NO: 24 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 695th to 1124th base sequences in the base sequence of SEQ ID NO: 24, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<24e ′> At least a part of the base sequence of 695 to 1124 in the base sequence of SEQ ID NO: 24 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1125 to 1801 in the base sequence of SEQ ID NO: 24, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<24f ′> A base sequence of at least a portion of the 695th to 1124th base sequences in the base sequence of SEQ ID NO: 24 or a complementary sequence of the equivalent base sequence as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 1125 to 1801 in the base sequence of SEQ ID NO: 24, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <24a> to <24f> and <24a ′> to <24f ′>, “the 1st to 694th base sequence in the base sequence of SEQ ID NO: 24” is “the 1st to 694th base in the base sequence of SEQ ID NO: 84” In the “sequence”, “the 1125th to 1801st base sequences in the base sequence of SEQ ID NO: 24” may be replaced with “the 695th to 1368th base sequences in the base sequence of SEQ ID NO: 84”, respectively.

  In addition to the combination of the four primers <24a '> to <24f'>, two loop primers (LF and LB) may be used in combination.

  The combination of the four primers <24a '> to <24f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <24a '> to <24b'> and any of <24c '> to <24f'>.

<25> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (25) In the base sequence of SEQ ID NO: 25, the insertion region of mPing is the nucleotide residues 729 to 1158 (FIG. 25). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (25) mPing, the first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of polymorphism (25), a combination of primers composed of the following primer pairs (eg, primers for PCR) and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<25a> a first primer having at least a partial base sequence of the 1st to 728th base sequence in the base sequence of SEQ ID NO: 25 or an equivalent base sequence thereof, and the 1159th to 1973th positions in the base sequence of SEQ ID NO: 25 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<25b> a first primer having at least a partial base sequence of the 1st to 728th base sequences in the base sequence of SEQ ID NO: 25 or a complementary sequence thereof, and 1159 in the base sequence of SEQ ID NO: 25 A combination of second primers having at least a part of the base sequence of the 1973 base sequence or an equivalent base sequence thereof;
<25c> a first primer having at least a partial base sequence of the 1st to 728th base sequence in the base sequence of SEQ ID NO: 25 or an equivalent base sequence thereof, and the 729th to 1158th positions of the base sequence of SEQ ID NO: 25 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<25d> a first primer having at least a partial base sequence of the 1st to 728th base sequence in the base sequence of SEQ ID NO: 25 or a complementary sequence thereof, and 729 to in the base sequence of SEQ ID NO: 25 A combination of second primers having at least a part of the base sequence of the 1158th base sequence or an equivalent base sequence thereof;
<25e> a first primer having at least a partial base sequence of the 729 to 1158th base sequence in the base sequence of SEQ ID NO: 25 or an equivalent base sequence thereof, and the 1159th to 1973th positions in the base sequence of SEQ ID NO: 25 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<25f> a first primer having at least a partial base sequence of the 729 to 1158th base sequence in the base sequence of SEQ ID NO: 25 or a complementary sequence thereof, and 1159 in the base sequence of SEQ ID NO: 25 A combination of second primers having at least a part of the base sequence of the 1973 base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (25) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<25a ′> At least a part of the 1st to 728th base sequences in the base sequence of SEQ ID NO: 25 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1159 to 1973 in the base sequence of SEQ ID NO: 25, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<25b ′> At least a part of the 1st to 728th base sequences in the base sequence of SEQ ID NO: 25, or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of 1159 to 1973 in the base sequence of SEQ ID NO: 25, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<25c ′> At least a part of the 1st to 728th base sequences in the base sequence of SEQ ID NO: 25 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of positions 729 to 1158 in the base sequence of SEQ ID NO: 4 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<25d '> A base sequence of at least a part of the 1st to 728th base sequences in the base sequence of SEQ ID NO: 25 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 729 to 1158th base sequence in the base sequence of SEQ ID NO: 25, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<25e ′> At least a part of the base sequence from the 729th to the 1158th base sequence in the base sequence of SEQ ID NO: 25 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1159 to 1973 in the base sequence of SEQ ID NO: 25, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<25f ′> At least a part of the base sequence from the 729th to 1158th base sequence in the base sequence of SEQ ID NO: 25 or a complementary sequence equivalent thereto is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of 1159 to 1973 in the base sequence of SEQ ID NO: 25, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <25a> to <25f> and <25a ′> to <25f ′>, “the 1st to 728th base sequence in the base sequence of SEQ ID NO: 25” is “the 1st to 728th base in the base sequence of SEQ ID NO: 85” In the “sequence”, “the 1159th to 1973th base sequence in the base sequence of SEQ ID NO: 25” may be replaced with “the 729th to 1540th base sequence in the base sequence of SEQ ID NO: 85”, respectively.

  In addition to the combination of the four primers <25a ′> to <25f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <25a '> to <25f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <25a ′> to <25b ′> and any of <25c ′> to <25f ′>.

<26> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (26);
In the base sequence of SEQ ID NO: 26, the mPing insertion region is a region consisting of nucleotide residues 657 to 1086 (FIG. 26). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (26) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of polymorphism (26), a combination of primers composed of the following primer pairs (eg, primers for PCR) and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<26a> a first primer having at least a part of the 1st to 656th base sequence in the base sequence of SEQ ID NO: 26 or an equivalent base sequence thereof, and the 1087th to 1762th positions of the base sequence of SEQ ID NO: 26 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<26b> a first primer having at least a partial base sequence of the 1st to 656th base sequence in the base sequence of SEQ ID NO: 26 or a complementary sequence of the equivalent base sequence, and 1087 to 10 in the base sequence of SEQ ID NO: 26 A combination of second primers having at least a part of the 1762th base sequence or an equivalent base sequence thereof;
<26c> a first primer having at least a part of the 1st to 656th base sequence in the base sequence of SEQ ID NO: 26 or an equivalent base sequence thereof, and the 657th to 1086th positions in the base sequence of SEQ ID NO: 26 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<26d> a first primer having at least a partial base sequence of the 1-656th base sequence in the base sequence of SEQ ID NO: 26 or a complementary sequence thereof, and 657- in the base sequence of SEQ ID NO: 26 A second primer combination having at least a part of the base sequence of the 1086th base sequence or an equivalent base sequence thereof;
<26e> a first primer having at least a part of a base sequence of the 657th to 1086th base sequence in the base sequence of SEQ ID NO: 26 or an equivalent base sequence thereof, and a 1087th to 1762th position of the base sequence of SEQ ID NO: 26 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<26f> a first primer having at least a part of a base sequence of the 657th to 1086th base sequences in the base sequence of SEQ ID NO: 26 or a complementary sequence thereof, and 1087 to 10 in the base sequence of SEQ ID NO: 26 A combination of second primers having at least a part of the 1762th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (26) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<26a ′> At least a part of the 1-656th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 26 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1087 to 1762 base sequence in the base sequence of SEQ ID NO: 26, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<26b ′> At least a part of the 1st to 656th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 26 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1087 to 1762th base sequence in the base sequence of SEQ ID NO: 26, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<26c ′> At least a part of the 1st to 656th base sequence in the base sequence of SEQ ID NO: 26 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 657 to 1086 in the base sequence of SEQ ID NO: 26, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<26d ′> At least a part of the 1st to 656th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 26 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 657th to 1086th base sequences in the base sequence of SEQ ID NO: 26, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<26e ′> A base sequence of at least a part of the 657th to 1086th base sequences in the base sequence of SEQ ID NO: 26 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1087 to 1762 base sequence in the base sequence of SEQ ID NO: 26, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<26f ′> A base sequence of at least a part of the 657th to 1086th base sequences in the base sequence of SEQ ID NO: 26 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1087 to 1762th base sequence in the base sequence of SEQ ID NO: 26, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c region or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <26a> to <26f> and <26a ′> to <26f ′>, “the 1st to 656th base sequence in the base sequence of SEQ ID NO: 26” is “the 1st to 656th base in the base sequence of SEQ ID NO: 86” In the “sequence”, “the 1087th to 1762th base sequence in the base sequence of SEQ ID NO: 26” may be replaced with “the 657th to 1329th base sequence in the base sequence of SEQ ID NO: 86”, respectively.

  In addition to the combination of the four primers <26a '> to <26f'>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <26a '> to <26f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <26a '> to <26b'> and any of <26c '> to <26f'>.

<27> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (27) In the base sequence of SEQ ID NO: 27, the insertion region of mPing is the nucleotide residues 704 to 1133 (FIG. 27). Therefore, as two or more primers for detecting the presence or absence of insertion of mPing of polymorphism (27), a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (27) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<27a> a first primer having at least a partial base sequence of the 1-703th base sequence in the base sequence of SEQ ID NO: 27 or an equivalent base sequence thereof, and the 1134-1888th base sequence of the base sequence of SEQ ID NO: 27 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<27b> a first primer having at least a partial base sequence of the 1st to 703th base sequences in the base sequence of SEQ ID NO: 27 or a complementary sequence thereof, and 1134 in the base sequence of SEQ ID NO: 27 A combination of second primers having at least a part of the base sequence of the 1888th base sequence or an equivalent base sequence thereof;
<27c> a first primer having at least a partial base sequence of the 1st to 703th base sequences in the base sequence of SEQ ID NO: 27 or an equivalent base sequence thereof, and the 704th to 1133rd positions of the base sequence of SEQ ID NO: 27 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<27d> a first primer having at least a partial base sequence of the 1st to 703th base sequences in the base sequence of SEQ ID NO: 27 or a complementary sequence thereof, and 704 in the base sequence of SEQ ID NO: 27 A combination of second primers having at least a part of the base sequence of the 1133rd base sequence or an equivalent base sequence thereof;
<27e> a first primer having at least a partial base sequence of the 704 to 1133th base sequence in the base sequence of SEQ ID NO: 27 or an equivalent base sequence thereof, and the 1134 to 1888th base sequences of the base sequence of SEQ ID NO: 27 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<27f> a first primer having at least a partial base sequence of the 704 to 1133rd base sequence in the base sequence of SEQ ID NO: 27 or a complementary sequence thereof, and 1134 in the base sequence of SEQ ID NO: 27 A combination of second primers having at least a part of the base sequence of the 1888th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (27) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<27a ′> At least a part of the base sequence from the 1st to the 703th base sequence in the base sequence of SEQ ID NO: 27 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence from 1134 to 1888 in the base sequence of SEQ ID NO: 27 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<27b ′> A base sequence of at least a part of the 1st to 703rd base sequences in the base sequence of SEQ ID NO: 27 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1134 to 1888th base sequence in the base sequence of SEQ ID NO: 27, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<27c ′> A base sequence of at least a part of the 1st to 703rd base sequence in the base sequence of SEQ ID NO: 27 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of positions 704 to 1133 in the base sequence of SEQ ID NO: 27 or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<27d ′> At least a part of the 1st to 703th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 27 or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of positions 704 to 1133 in the base sequence of SEQ ID NO: 27, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<27e ′> At least a part of the base sequence of positions 704 to 1133 in the base sequence of SEQ ID NO: 27, or an equivalent base sequence thereof, is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence from 1134 to 1888 in the base sequence of SEQ ID NO: 27 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<27f ′> A base sequence of at least a part of the 704th to 1133rd base sequences in the base sequence of SEQ ID NO: 27 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1134 to 1888th base sequence in the base sequence of SEQ ID NO: 27, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <27a> to <27f> and <27a ′> to <27f ′>, “the 1st to 703rd base sequence in the base sequence of SEQ ID NO: 27” is “the 1st to 703th base in the base sequence of SEQ ID NO: 87” In the “sequence”, “the 1134th to 1888th base sequence in the base sequence of SEQ ID NO: 27” may be replaced with “the 704th to 1455th base sequence in the base sequence of SEQ ID NO: 87”, respectively.

  In addition to the four primer combinations <27a ′> to <27f ′>, two loop primers (LF and LB) may be further used in combination.

  The four primer combinations <27a ′> to <27f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <27a '> to <27b'> and any of <27c '> to <27f'>.

<28> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (28);
In the base sequence of SEQ ID NO: 28, the mPing insertion region is a region consisting of nucleotide residues 705 to 1134 (FIG. 28). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (28) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (28) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations). PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<28a> a first primer having at least a partial base sequence of the 1st to 704th base sequences in the base sequence of SEQ ID NO: 28 or an equivalent base sequence thereof, and 1135 to 1840th in the base sequence of SEQ ID NO: 28 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<28b> a first primer having at least a partial base sequence of the 1st to 704th base sequences in the base sequence of SEQ ID NO: 28 or a complementary sequence thereof, and 1135 in the base sequence of SEQ ID NO: 28 A combination of second primers having at least a part of the base sequence of the 1840th base sequence or an equivalent base sequence thereof;
<28c> a first primer having at least a partial base sequence of the 1st to 704th base sequences in the base sequence of SEQ ID NO: 28 or an equivalent base sequence thereof, and the 705th to 1134th positions of the base sequence of SEQ ID NO: 28 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<28d> a first primer having at least a partial base sequence of the 1st to 704th base sequences in the base sequence of SEQ ID NO: 28 or a complementary sequence thereof, and 705 to 705 in the base sequence of SEQ ID NO: 28 A combination of second primers having at least part of the 1134th base sequence or an equivalent base sequence thereof;
<28e> a first primer having at least a partial base sequence of the 705 to 1134th base sequence in the base sequence of SEQ ID NO: 28 or an equivalent base sequence thereof, and the 1135th to 1840th positions in the base sequence of SEQ ID NO: 28 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<28f> a first primer having at least a partial base sequence of the 705 to 1134th base sequence in the base sequence of SEQ ID NO: 28 or a complementary sequence thereof, and 1135 in the base sequence of SEQ ID NO: 28 A combination of second primers having at least a part of the base sequence of the 1840th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (28) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<28a ′> The base sequence of at least a part of the 1st to 704th base sequences in the base sequence of SEQ ID NO: 28 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1135 to 1840th base sequence in the base sequence of SEQ ID NO: 28, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<28b '> A base sequence of at least a part of the 1st to 704th base sequences in the base sequence of SEQ ID NO: 28 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1135th to 1840th base sequences in the base sequence of SEQ ID NO: 28, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<28c ′> At least a part of the 1st to 704th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 28 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the nucleotide sequence of 705 to 1134 in the nucleotide sequence of SEQ ID NO: 28, or a complementary sequence of its equivalent nucleotide sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<28d '> A base sequence of at least a part of the 1st to 704th base sequences in the base sequence of SEQ ID NO: 28 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 705 to 1134th base sequence in the base sequence of SEQ ID NO: 28 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<28e '> A base sequence of at least a part of the 705 to 1134th base sequence in the base sequence of SEQ ID NO: 28 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1135 to 1840th base sequence in the base sequence of SEQ ID NO: 28, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<28f '> A base sequence of at least a part of the 705 to 1134th base sequence in the base sequence of SEQ ID NO: 28 or a complementary sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1135th to 1840th base sequences in the base sequence of SEQ ID NO: 28, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <28a> to <28f> and <28a ′> to <28f ′>, “the 1st to 704th base sequence in the base sequence of SEQ ID NO: 28” is “the 1st to 704th base in the base sequence of SEQ ID NO: 88” In the “sequence”, “the 1535th to 1840th base sequence in the base sequence of SEQ ID NO: 28” may be replaced with “the 705th to 1407th base sequence in the base sequence of SEQ ID NO: 88”, respectively.

  In addition to the four primer combinations <28a ′> to <28f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <28a '> to <28f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <28a '> to <28b'> and any of <28c '> to <28f'>.

<29> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (29) In the base sequence of SEQ ID NO: 29, the insertion region of mPing is the 697th to 1126th nucleotide residue (FIG. 29). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (29) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of the polymorphism (29), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<29a> a first primer having at least a part of the 1-696th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 29 or an equivalent nucleotide sequence thereof, and the 1127th-1767th nucleotide in the nucleotide sequence of SEQ ID NO: 29 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<29b> a first primer having at least a partial base sequence of the 1st to 696th base sequence in the base sequence of SEQ ID NO: 29 or a complementary sequence thereof, and 1127 in the base sequence of SEQ ID NO: 29 A combination of second primers having at least a part of the base sequence of the 1767th base sequence or an equivalent base sequence thereof;
<29c> a first primer having at least a part of the 1-696th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 29 or an equivalent nucleotide sequence thereof, and the 697-1126th nucleotide in the nucleotide sequence of SEQ ID NO: 29 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<29d> a first primer having at least a partial base sequence of the 1st to 696th base sequences in the base sequence of SEQ ID NO: 29 or a complementary sequence thereof, and 697 to 6 in the base sequence of SEQ ID NO: 29 A combination of second primers having at least a part of the base sequence of the 1126th base sequence or an equivalent base sequence thereof;
<29e> a first primer having at least a partial base sequence of the 697 to 1126th base sequence in the base sequence of SEQ ID NO: 29 or an equivalent base sequence thereof, and the 1127th to 1767th positions in the base sequence of SEQ ID NO: 29 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<29f> a first primer having at least a partial base sequence of the 697 to 1126th base sequence in the base sequence of SEQ ID NO: 29 or a complementary sequence thereof, and 1127 in the base sequence of SEQ ID NO: 29 A combination of second primers having at least a part of the base sequence of the 1767th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (29) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<29a ′> At least a part of the 1-696th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 29 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1127 to 1767 in the base sequence of SEQ ID NO: 29 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<29b ′> A base sequence of at least a part of the 1st to 696th base sequence in the base sequence of SEQ ID NO: 29 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the 1127 to 1767th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 29 or an equivalent nucleotide sequence thereof A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<29c ′> At least a part of the 1-696th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 29 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the 697 to 1126th base sequence in the base sequence of SEQ ID NO: 4 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<29d ′> A base sequence of at least a part of the 1st to 696th base sequences in the base sequence of SEQ ID NO: 29 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 697 to 1126th base sequence in the base sequence of SEQ ID NO: 29 or an equivalent base sequence thereof A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<29e ′> At least a part of the 697 to 1126th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 29 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1127 to 1767 in the base sequence of SEQ ID NO: 29 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<29f ′> At least a part of the 697 to 1126th base sequence in the base sequence of SEQ ID NO: 29, or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the 1127 to 1767th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 29 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <29a> to <29f> and <29a ′> to <29f ′>, “the 1st to 696th base sequence in the base sequence of SEQ ID NO: 29” is “the 1st to 696th base in the base sequence of SEQ ID NO: 89” In the “sequence”, “the 1127th to 1767th base sequence in the base sequence of SEQ ID NO: 29” may be replaced with “the 697th to 1334th base sequence in the base sequence of SEQ ID NO: 89”.

  In addition to the four primer combinations <29a '> to <29f'>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <29a '> to <29f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <29a '> to <29b'> and any of <29c '> to <29f'>.

<30> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (30);
In the base sequence of SEQ ID NO: 30, the mPing insertion region is a region consisting of nucleotide residues 801 to 1230 (FIG. 30). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (30) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (30) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such primer combinations (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<30a> a first primer having at least a part of a base sequence of 1 to 800 in the base sequence of SEQ ID NO: 30 or an equivalent base sequence thereof, and a 1231 to 1912th base in the base sequence of SEQ ID NO: 30 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<30b> a first primer having at least a partial base sequence of the 1st to 800th base sequences in the base sequence of SEQ ID NO: 30 or a complementary sequence of the equivalent base sequence, and 1231 in the base sequence of SEQ ID NO: 30 A combination of second primers having at least a part of the base sequence of the 1912th base sequence or an equivalent base sequence thereof;
<30c> a first primer having at least a part of the base sequence of 1 to 800 in the base sequence of SEQ ID NO: 30 or an equivalent base sequence thereof, and the 801 to 1230 th in the base sequence of SEQ ID NO: 30 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<30d> a first primer having at least a partial base sequence of the 1st to 800th base sequences in the base sequence of SEQ ID NO: 30 or a complementary sequence thereof, and 801 to 801 in the base sequence of SEQ ID NO: 30 A second primer combination having at least a part of the base sequence of the 1230th base sequence or an equivalent base sequence thereof;
<30e> a first primer having at least a partial base sequence of the 801 to 1230th base sequence in the base sequence of SEQ ID NO: 30 or an equivalent base sequence thereof, and the 1231 to 1912th base sequences of the base sequence of SEQ ID NO: 30 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<30f> a first primer having at least a part of the base sequence of the 801 to 1230th base sequence in the base sequence of SEQ ID NO: 30 or a complementary sequence thereof, and 1231 in the base sequence of SEQ ID NO: 30 A combination of second primers having at least a part of the base sequence of the 1912th base sequence or an equivalent base sequence thereof.

The LAMP primer set that can detect the presence or absence of insertion of the polymorphic (30) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<30a ′> At least a part of the 1st to 800th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 30 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1231 to 1912 in the base sequence of SEQ ID NO: 30 or a complementary sequence equivalent thereto, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<30b ′> At least a part of the 1st to 800th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 30 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1231st to 1912th base sequences in the base sequence of SEQ ID NO: 30 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<30c ′> At least a part of the 1st to 800th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 30 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of positions 801 to 1230 in the base sequence of SEQ ID NO: 30, or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<30d ′> At least a part of the 1st to 800th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 30 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the base sequence of 801 to 1230 in the base sequence of SEQ ID NO: 30 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<30e ′> At least a part of the base sequence of the 801 to 1230th base sequence in the base sequence of SEQ ID NO: 30 or an equivalent base sequence thereof as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1231 to 1912 in the base sequence of SEQ ID NO: 30 or a complementary sequence equivalent thereto, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<30f ′> At least a part of the base sequence of the 801 to 1230th base sequence in the base sequence of SEQ ID NO: 30 or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1231st to 1912th base sequences in the base sequence of SEQ ID NO: 30 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <30a> to <30f> and <30a ′> to <30f ′>, “the 1st to 800th base sequence in the base sequence of SEQ ID NO: 30” is “the 1st to 800th base in the base sequence of SEQ ID NO: 90” In the “sequence”, the “1231 to 1912th base sequence in the base sequence of SEQ ID NO: 30” may be replaced with “801 to 1479th base sequence in the base sequence of SEQ ID NO: 90”, respectively.

  In addition to the four primer combinations <30a ′> to <30f ′>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <30a '> to <30f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <30a '> to <30b'> and any of <30c '> to <30f'>.

<31> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (31) In the base sequence of SEQ ID NO: 31, the insertion region of mPing is the nucleotide residues 748 to 1177 (FIG. 31). Therefore, as two or more primers for detecting the presence or absence of insertion of mPing of the polymorphism (31), the first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (31) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<31a> a first primer having at least a part of the 1-747th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 31 or an equivalent nucleotide sequence thereof, and the 1178-1756th nucleotide in the nucleotide sequence of SEQ ID NO: 31 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<31b> a first primer having at least a partial base sequence of the 1-747th base sequence in the base sequence of SEQ ID NO: 31 or a complementary sequence of the equivalent base sequence, and 1178- in the base sequence of SEQ ID NO: 31 A combination of second primers having at least a part of the base sequence of the 1756th base sequence or an equivalent base sequence thereof;
<31c> a first primer having at least a part of the 1-747th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 31 or an equivalent nucleotide sequence thereof, and the 748-1177th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 31 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<31d> a first primer having at least a partial base sequence of the 1-747th base sequence in the base sequence of SEQ ID NO: 31 or a complementary sequence of the equivalent base sequence, and 748- in the base sequence of SEQ ID NO: 31 A combination of second primers having at least a part of the base sequence of the 1177th base sequence or an equivalent base sequence thereof;
<31e> a first primer having at least a part of a base sequence of 748 to 1177th in the base sequence of SEQ ID NO: 31 or an equivalent base sequence thereof, and a first primer of 1178 to 1756th in the base sequence of SEQ ID NO: 31 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<31f> a first primer having at least a part of a base sequence of the 748 to 1177th base sequence in the base sequence of SEQ ID NO: 31 or a complementary sequence of the equivalent base sequence, and 1178 in the base sequence of SEQ ID NO: 31 A combination of second primers having at least a part of the 1756th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (31) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<31a ′> At least a part of the 1-747th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 31 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1178 to 1756th base sequence in the base sequence of SEQ ID NO: 31 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<31b ′> At least a part of the 1-747th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 31 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1178th to 1756th base sequence in the base sequence of SEQ ID NO: 31 or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<31c ′> At least a part of the 1-747th base sequence in the base sequence of SEQ ID NO: 31 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 748 to 1177th base sequence in the base sequence of SEQ ID NO: 31 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<31d ′> A base sequence of at least a part of the 1st to 747th base sequences in the base sequence of SEQ ID NO: 31 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 748 to 1177th base sequence in the base sequence of SEQ ID NO: 31 or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<31e ′> A base sequence of at least a part of the base sequence from 748 to 1177 in the base sequence of SEQ ID NO: 31 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1178 to 1756th base sequence in the base sequence of SEQ ID NO: 31 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<31f ′> A base sequence of at least a part of the 748 to 1177th base sequence in the base sequence of SEQ ID NO: 31 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1178th to 1756th base sequence in the base sequence of SEQ ID NO: 31 or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <31a> to <31f> and <31a ′> to <31f ′>, “the 1st to 747th base sequence in the base sequence of SEQ ID NO: 31” is “the 1st to 747th base in the base sequence of SEQ ID NO: 91” In the “sequence”, “the 1178th to 1756th base sequence in the base sequence of SEQ ID NO: 31” may be replaced by “the 748th to 1323th base sequence in the base sequence of SEQ ID NO: 91”, respectively.

  In addition to the four primer combinations <31a ′> to <31f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <31a '> to <31f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <31a '> to <31b'> and any of <31c '> to <31f'>.

<32> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (32);
In the base sequence of SEQ ID NO: 32, the mPing insertion region is a region consisting of nucleotide residues 723 to 1152 (FIG. 32). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (32) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (32) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations). PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<32a> a first primer having at least a partial base sequence of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 32 or an equivalent base sequence thereof, and the 1153th to 1872nd positions of the base sequence of SEQ ID NO: 32 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<32b> a first primer having at least a partial base sequence of the 1st to 722nd base sequences in the base sequence of SEQ ID NO: 32 or a complementary sequence thereof, and 1153 in the base sequence of SEQ ID NO: 32 A combination of second primers having at least a part of the base sequence of the 1872nd base sequence or an equivalent base sequence thereof;
<32c> a first primer having at least a partial base sequence of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 32 or an equivalent base sequence thereof, and the 723 to 1152nd positions of the base sequence of SEQ ID NO: 32 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<32d> a first primer having at least a partial base sequence of the 1st to 722nd base sequences in the base sequence of SEQ ID NO: 32 or a complementary sequence of the equivalent base sequence, and 723 in the base sequence of SEQ ID NO: 32 A combination of second primers having at least a part of the 1152nd base sequence or an equivalent base sequence thereof;
<32e> a first primer having at least a partial base sequence of the 723 to 1152 base sequences in the base sequence of SEQ ID NO: 32 or an equivalent base sequence thereof, and the 1153 to 1862 th base sequences of the base sequence of SEQ ID NO: 32 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<32f> a first primer having at least a partial base sequence of the 723 to 1152 base sequences in the base sequence of SEQ ID NO: 32 or a complementary sequence of the equivalent base sequence, and 1153 in the base sequence of SEQ ID NO: 32 A combination of second primers having at least part of the base sequence of the 1872nd base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (32) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<32a ′> At least a part of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 32 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1153 to 1872 in the base sequence of SEQ ID NO: 32 or a complementary sequence of the equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<32b ′> At least a part of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 32 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of 1153 to 1872 in the base sequence of SEQ ID NO: 32, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<32c ′> At least a part of the 1st to 722nd base sequences in the base sequence of SEQ ID NO: 32 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 723 to 1152 in the base sequence of SEQ ID NO: 32, or a complementary sequence of the equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<32d ′> At least a part of the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 32, or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 723 to 1152 base sequences in the base sequence of SEQ ID NO: 32, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<32e ′> A base sequence of at least a part of the 723 to 1152 base sequences in the base sequence of SEQ ID NO: 32 or an equivalent base sequence thereof as an F1c region or F2 region in FIP or as an F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1153 to 1872 in the base sequence of SEQ ID NO: 32 or a complementary sequence of the equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<32f ′> At least a part of the base sequence of the 723 to 1152 base sequence in the base sequence of SEQ ID NO: 32 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of 1153 to 1872 in the base sequence of SEQ ID NO: 32, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <32a> to <32f> and <32a ′> to <32f ′>, “the 1st to 722nd base sequence in the base sequence of SEQ ID NO: 32” is “the 1st to 722th base in the base sequence of SEQ ID NO: 92” In “Sequence”, “the 1153-1857th base sequence in the base sequence of SEQ ID NO: 32” may be replaced with “723-1439th base sequence in the base sequence of SEQ ID NO: 92”.

  In addition to the combination of the four primers <32a ′> to <32f ′>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <32a ′> to <32f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <32a '> to <32b'> and any of <32c '> to <32f'>.

<33> Primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (33) In the base sequence of SEQ ID NO: 33, the insertion region of mPing is the 651-1080th nucleotide residue (FIG. 33). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (33) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (33) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<33a> a first primer having at least a partial base sequence of the 1st to 650th base sequences in the base sequence of SEQ ID NO: 33 or an equivalent base sequence thereof, and the 1081st to 1748th positions of the base sequence of SEQ ID NO: 33 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<33b> a first primer having at least a partial base sequence of the 1st to 650th base sequences in the base sequence of SEQ ID NO: 33 or a complementary sequence thereof, and 1081 in the base sequence of SEQ ID NO: 33 A combination of second primers having at least part of the 1748th base sequence or an equivalent base sequence thereof;
<33c> a first primer having at least a part of the base sequence of 1 to 650th in the base sequence of SEQ ID NO: 33 or an equivalent base sequence thereof, and the 651-1080th of the base sequence of SEQ ID NO: 33 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<33d> a first primer having at least a partial base sequence of the 1st to 650th base sequences in the base sequence of SEQ ID NO: 33 or a complementary sequence of the equivalent base sequence, and 651 in the base sequence of SEQ ID NO: 33 A combination of second primers having at least a part of the base sequence of the 1080th base sequence or an equivalent base sequence thereof;
<33e> a first primer having at least a partial base sequence of the 651 to 1080th base sequence in the base sequence of SEQ ID NO: 33 or an equivalent base sequence thereof, and the 1081 to 1748th positions of the base sequence of SEQ ID NO: 33 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<33f> a first primer having at least a partial base sequence of the 651-1080th base sequence in the base sequence of SEQ ID NO: 33 or a complementary sequence of the equivalent base sequence, and 1081- in the base sequence of SEQ ID NO: 33 A combination of second primers having at least a part of the 1748th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (33) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<33a ′> A base sequence of at least a part of the 1st to 650th base sequences in the base sequence of SEQ ID NO: 33 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of the 1081-1748th base sequence in the base sequence of SEQ ID NO: 33, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<33b ′> A base sequence of at least a part of the 1st to 650th base sequences in the base sequence of SEQ ID NO: 33 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1081-1748th base sequence in the base sequence of SEQ ID NO: 33, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<33c ′> At least a part of the 1st to 650th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 33 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 651 to 1080 in the base sequence of SEQ ID NO: 33 or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<33d ′> A base sequence of at least a part of the 1st to 650th base sequences in the base sequence of SEQ ID NO: 33 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 651 to 1080th base sequence in the base sequence of SEQ ID NO: 33, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<33e ′> A base sequence of at least a part of the 651-1080th base sequence in the base sequence of SEQ ID NO: 33 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of the 1081-1748th base sequence in the base sequence of SEQ ID NO: 33, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<33f ′> A base sequence of at least a part of the 651-1080th base sequence in the base sequence of SEQ ID NO: 33 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1081-1748th base sequence in the base sequence of SEQ ID NO: 33, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <33a> to <33f> and <33a ′> to <33f ′>, “the 1st to 650th base sequence in the base sequence of SEQ ID NO: 33” is “the 1st to 650th base in the base sequence of SEQ ID NO: 93” In "Sequence", "1081-1748th base sequence in the base sequence of SEQ ID NO: 33" may be replaced with "651th to 1315th base sequence in the base sequence of SEQ ID NO: 93".

  In addition to the four primer combinations <33a '> to <33f'>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <33a '> to <33f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <33a '> to <33b'> and any of <33c '> to <33f'>.

<34> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (34);
In the base sequence of SEQ ID NO: 34, the mPing insertion region is a region consisting of nucleotide residues 734 to 1163 (FIG. 34). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (34) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (34) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<34a> a first primer having at least a partial base sequence of the 1st to 733th base sequence in the base sequence of SEQ ID NO: 34 or an equivalent base sequence thereof, and the 1164th to 1879th positions of the base sequence of SEQ ID NO: 34 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<34b> a first primer having at least a partial base sequence of the 1st to 733th base sequences in the base sequence of SEQ ID NO: 34, or a complementary sequence of the equivalent base sequence, and 1164-in the base sequence of SEQ ID NO: 34 A second primer combination having at least a part of the base sequence of the 1879th base sequence or an equivalent base sequence thereof;
<34c> a first primer having at least a part of the 1-733 th base sequence in the base sequence of SEQ ID NO: 34 or an equivalent base sequence thereof, and the 734 thru 1163 th positions in the base sequence of SEQ ID NO: 34 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<34d> a first primer having at least a partial base sequence of the 1st to 733th base sequences in the base sequence of SEQ ID NO: 34 or a complementary sequence thereof, and 734 to A combination of second primers having at least a part of the base sequence of the 1163rd base sequence or an equivalent base sequence thereof;
<34e> a first primer having at least a partial base sequence of the 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 34 or an equivalent base sequence thereof, and the 1164th to 1879th positions in the base sequence of SEQ ID NO: 34 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<34f> a first primer having at least a partial base sequence of the 734th to 1163rd base sequence in the base sequence of SEQ ID NO: 34 or a complementary sequence of the equivalent base sequence; A combination of second primers having at least a part of the base sequence of the 1879th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (34) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<34a ′> At least a part of the 1-733th base sequence in the base sequence of SEQ ID NO: 34, or an equivalent base sequence thereof, as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1164 to 1879th base sequence in the base sequence of SEQ ID NO: 34 or a complementary sequence of the equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<34b ′> At least a part of the 1-733th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 34 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 1164 to 1879 in the base sequence of SEQ ID NO: 34, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<34c ′> A base sequence of at least a part of the base sequence 1 to 733 in the base sequence of SEQ ID NO: 34 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 34, or a complementary sequence thereof in BIP, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<34d ′> At least a part of the 1-733th base sequence in the base sequence of SEQ ID NO: 34, or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 34 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<34e ′> A base sequence of at least a part of the 734th to 1163rd base sequences in the base sequence of SEQ ID NO: 34 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1164 to 1879th base sequence in the base sequence of SEQ ID NO: 34 or a complementary sequence of the equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<34f ′> A base sequence of at least a part of the 734th to 1163rd base sequences in the base sequence of SEQ ID NO: 34 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 1164 to 1879 in the base sequence of SEQ ID NO: 34, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <34a> to <34f> and <34a ′> to <34f ′>, “the 1st to 733th base sequence in the base sequence of SEQ ID NO: 34” is “the 1st to 733th base in the base sequence of SEQ ID NO: 94” In the “sequence”, “the 1164th to 1879th base sequence in the base sequence of SEQ ID NO: 34” may be replaced with “the 734th to 1446th base sequence in the base sequence of SEQ ID NO: 94”, respectively.

  In addition to the four primer combinations <34a '> to <34f'>, two loop primers (LF and LB) may be further used in combination.

  The four primer combinations <34a '> to <34f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <34a '> to <34b'> and any of <34c '> to <34f'>.

<35> Primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (35) In the base sequence of SEQ ID NO: 35, the insertion region of mPing is the nucleotide residues 579 to 1008 (FIG. 35). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (35) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of the polymorphism (35), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<35a> a first primer having at least a part of the 1st to 578th base sequence in the base sequence of SEQ ID NO: 35 or an equivalent base sequence thereof, and the 1009th to 1687th positions of the base sequence of SEQ ID NO: 35 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<35b> a first primer having at least a partial base sequence of the 1st to 578th base sequence in the base sequence of SEQ ID NO: 35 or a complementary sequence thereof, and 1009 to 1009 in the base sequence of SEQ ID NO: 35 A second primer combination having at least a part of the base sequence of the 1687th base sequence or an equivalent base sequence thereof;
<35c> a first primer having at least a partial base sequence of the 1st to 578th base sequence in the base sequence of SEQ ID NO: 35 or an equivalent base sequence thereof, and the 579th to 1008th positions in the base sequence of SEQ ID NO: 35 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<35d> a first primer having at least a partial base sequence of the 1st to 578th base sequences in the base sequence of SEQ ID NO: 35 or a complementary sequence thereof, and 579 to in the base sequence of SEQ ID NO: 35 A second primer combination having at least a part of the base sequence of the 1008th base sequence or an equivalent base sequence thereof;
<35e> a first primer having at least a part of the base sequence of the 579 to 1008th base sequence in the base sequence of SEQ ID NO: 35 or an equivalent base sequence thereof, and the 1009th to 1687th positions of the base sequence of SEQ ID NO: 35 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<35f> a first primer having at least a part of the base sequence of the 579 to 1008th base sequence in the base sequence of SEQ ID NO: 35 or a complementary sequence of the equivalent base sequence, and 1009 to 1009 in the base sequence of SEQ ID NO: 35 A combination of second primers having at least a part of the base sequence of the 1687th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (35) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<35a ′> At least a part of the base sequence of the 1st to 578th base sequence in the base sequence of SEQ ID NO: 35 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of positions 1009 to 1687 in the base sequence of SEQ ID NO: 35, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<35b ′> A base sequence of at least a part of the 1st to 578th base sequence in the base sequence of SEQ ID NO: 35 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1009th to 1687th base sequences in the base sequence of SEQ ID NO: 35, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<35c ′> At least a part of the 1st to 578th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 35 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 579 to 1008 in the base sequence of SEQ ID NO: 4 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<35d '> A base sequence of at least a part of the 1st to 578th base sequences in the base sequence of SEQ ID NO: 35 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the base sequence of 579 to 1008 in the base sequence of SEQ ID NO: 35 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<35e ′> At least a part of the base sequence of the 579 to 1008th base sequence in the base sequence of SEQ ID NO: 35 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of positions 1009 to 1687 in the base sequence of SEQ ID NO: 35, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<35f ′> A base sequence of at least a part of the 579th to 1008th base sequences in the base sequence of SEQ ID NO: 35 or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1009th to 1687th base sequences in the base sequence of SEQ ID NO: 35, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <35a> to <35f> and <35a ′> to <35f ′>, “the 1st to 578th base sequence in the base sequence of SEQ ID NO: 35” is “1st to 578th base in the base sequence of SEQ ID NO: 95” In “Sequence”, “1009th to 1687th base sequence in the base sequence of SEQ ID NO: 35” may be replaced with “579th to 1254th base sequence in the base sequence of SEQ ID NO: 95”, respectively.

  In addition to the combination of the four primers <35a ′> to <35f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <35a ′> to <35f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <35a '> to <35b'> and any of <35c '> to <35f'>.

<36> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (36);
In the base sequence of SEQ ID NO: 36, the insertion region of mPing is a region consisting of nucleotide residues from 880 to 1309 (FIG. 36). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (36) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (36) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<36a> a first primer having at least a part of the 1-879th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 36 or an equivalent nucleotide sequence thereof, and the 1307th to 1925th nucleotides in the nucleotide sequence of SEQ ID NO: 36 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<36b> a first primer having at least a partial base sequence of the 1st to 879th base sequence in the base sequence of SEQ ID NO: 36 or a complementary sequence thereof, and 1307 to 1307 in the base sequence of SEQ ID NO: 36 A second primer combination having at least a part of the base sequence of the 1925th base sequence or an equivalent base sequence thereof;
<36c> a first primer having at least a partial base sequence of the 1st to 879th base sequence in the base sequence of SEQ ID NO: 36 or an equivalent base sequence thereof, and the 880th to 1309th positions in the base sequence of SEQ ID NO: 36 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<36d> a first primer having at least a partial base sequence of the 1st to 879th base sequence in the base sequence of SEQ ID NO: 36 or a complementary sequence of the equivalent base sequence, and 880 in the base sequence of SEQ ID NO: 36 A combination of second primers having at least a part of the base sequence of the 1309th base sequence or an equivalent base sequence thereof;
<36e> a first primer having at least a partial base sequence of the 880 to 1309th base sequence in the base sequence of SEQ ID NO: 36 or an equivalent base sequence thereof, and the 1307th to 1925th positions in the base sequence of SEQ ID NO: 36 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<36f> a first primer having at least a partial base sequence of the 880 to 1309th base sequence in the base sequence of SEQ ID NO: 36 or a complementary sequence of the equivalent base sequence, and 1307 to 3 in the base sequence of SEQ ID NO: 36 A combination of second primers having at least a part of the base sequence of the 1925th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (36) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<36a ′> At least a part of the 1-879th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 36 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the nucleotide sequence of 1307 to 1925 in the nucleotide sequence of SEQ ID NO: 36, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<36b ′> At least a part of the base sequence of the 1st to 879th base sequence in the base sequence of SEQ ID NO: 36 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1307th to 1925th base sequences in the base sequence of SEQ ID NO: 36, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<36c ′> At least a part of the 1-879th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 36 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 880 to 1309 in the base sequence of SEQ ID NO: 36, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<36d ′> At least a part of the 1-879th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 36 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 880 to 1309 in the base sequence of SEQ ID NO: 36, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<36e ′> The base sequence of at least a part of the base sequence from 880 to 1309 in the base sequence of SEQ ID NO: 36 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the nucleotide sequence of 1307 to 1925 in the nucleotide sequence of SEQ ID NO: 36, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<36f ′> A base sequence of at least a part of the base sequence from 880 to 1309 in the base sequence of SEQ ID NO: 36 or a complementary sequence of the equivalent base sequence is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1307th to 1925th base sequences in the base sequence of SEQ ID NO: 36, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <36a> to <36f> and <36a ′> to <36f ′>, “the 1st to 879th base sequence in the base sequence of SEQ ID NO: 36” is “1st to 879th base in the base sequence of SEQ ID NO: 96” In the “sequence”, “the 1307th to 1925th base sequence in the base sequence of SEQ ID NO: 36” may be replaced with “the 880th to 1492th base sequence in the base sequence of SEQ ID NO: 96”, respectively.

  In addition to the four primer combinations <36a ′> to <36f ′>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <36a '> to <36f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <36a '> to <36b'> and any of <36c '> to <36f'>.

<37> Primer set comprising two or more primers capable of detecting the presence or absence of insertion of mPing in the polymorphism (37) In the base sequence of SEQ ID NO: 37, the insertion region of mPing is the nucleotide residues 732 to 1161 (FIG. 37). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (37) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of the polymorphism (37), a combination of primers composed of the following primer pairs (eg, primers for PCR) and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<37a> a first primer having at least a part of the base sequence of 1-731 in the base sequence of SEQ ID NO: 37 or an equivalent base sequence thereof, and the 1162 to 1867th of the base sequence of SEQ ID NO: 37 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<37b> a first primer having at least a partial base sequence of the 1-731st base sequence in the base sequence of SEQ ID NO: 37 or a complementary sequence of the equivalent base sequence, and 1162 in the base sequence of SEQ ID NO: 37 A combination of second primers having at least a part of the base sequence of the 1867th base sequence or an equivalent base sequence thereof;
<37c> a first primer having at least a part of the base sequence of 1 to 731 in the base sequence of SEQ ID NO: 37 or an equivalent base sequence thereof, and the 732 to 1161 th positions in the base sequence of SEQ ID NO: 37 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<37d> a first primer having at least a partial base sequence of the 1st to 731st base sequences in the base sequence of SEQ ID NO: 37 or a complementary sequence of the equivalent base sequence, and 732 in the base sequence of SEQ ID NO: 37 A combination of second primers having at least part of the 1161st base sequence or an equivalent base sequence thereof;
<37e> a first primer having at least a partial base sequence of the 732 to 1161st base sequences in the base sequence of SEQ ID NO: 37 or an equivalent base sequence thereof, and the 1162 to 1867th positions of the base sequence of SEQ ID NO: 37 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<37f> a first primer having at least a partial base sequence of the 732 to 1161st base sequences in the base sequence of SEQ ID NO: 37 or a complementary sequence thereof, and 1162 in the base sequence of SEQ ID NO: 37 A combination of second primers having at least a part of the base sequence of the 1867th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (37) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<37a ′> A base sequence of at least a part of the base sequence from 1 to 731 in the base sequence of SEQ ID NO: 37 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the 1162-1867th base sequence in the base sequence of SEQ ID NO: 37, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<37b ′> At least a part of the base sequence of the 1st to 731st base sequences in the base sequence of SEQ ID NO: 37 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1162 to 1867th base sequence in the base sequence of SEQ ID NO: 37 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<37c ′> A base sequence of at least a part of the 1st to 731st base sequences in the base sequence of SEQ ID NO: 37 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 732 to 1161 in the base sequence of SEQ ID NO: 37, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<37d ′> A base sequence of at least a part of the 1st to 731st base sequences in the base sequence of SEQ ID NO: 37 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 732 to 1161 in the base sequence of SEQ ID NO: 37, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<37e ′> At least a part of the 732 to 1161st nucleotide sequences in the nucleotide sequence of SEQ ID NO: 37 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the 1162-1867th base sequence in the base sequence of SEQ ID NO: 37, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<37f ′> A base sequence of at least a part of the 732 to 1161st base sequences in the base sequence of SEQ ID NO: 37 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1162 to 1867th base sequence in the base sequence of SEQ ID NO: 37 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <37a> to <37f> and <37a ′> to <37f ′>, “the 1st to 731st base sequence in the base sequence of SEQ ID NO: 37” is “the 1st to 718th base in the base sequence of SEQ ID NO: 97” In the “sequence”, “the 1162nd to 1867th base sequence in the base sequence of SEQ ID NO: 37” may be replaced with “719th to 1377th positions in the base sequence of SEQ ID NO: 97”, respectively.

  In addition to the four primer combinations <37a ′> to <37f ′>, two loop primers (LF and LB) may be used in combination.

  The combination of the four primers <37a '> to <37f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <37a '> to <37b'> and any of <37c '> to <37f'>.

<38> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (38);
In the base sequence of SEQ ID NO: 38, the insertion region of mPing is a region consisting of nucleotide residues 757 to 1186 (FIG. 38). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (38) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (38) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<38a> a first primer having at least a partial base sequence of the 1st to 756th base sequence in the base sequence of SEQ ID NO: 38 or an equivalent base sequence thereof, and the 1187th to 1773rd positions of the base sequence of SEQ ID NO: 38 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<38b> a first primer having at least a partial base sequence of the 1st to 756th base sequence in the base sequence of SEQ ID NO: 38 or a complementary sequence thereof, and 1187 to A second primer combination having at least a part of the base sequence of the 1773th base sequence or an equivalent base sequence thereof;
<38c> a first primer having at least a partial base sequence of the 1st to 756th base sequence in the base sequence of SEQ ID NO: 38 or an equivalent base sequence thereof, and the 757th to 1186th positions of the base sequence of SEQ ID NO: 38 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<38d> a first primer having at least a partial base sequence of the 1st to 756th base sequence in the base sequence of SEQ ID NO: 38 or a complementary sequence of the equivalent base sequence, and 757 in the base sequence of SEQ ID NO: 38 A combination of second primers having at least part of the base sequence of the 1186th base sequence or an equivalent base sequence thereof;
<38e> a first primer having at least a partial base sequence of the 757 to 1186th base sequence in the base sequence of SEQ ID NO: 38 or an equivalent base sequence thereof, and the 1187 to 1773rd positions of the base sequence of SEQ ID NO: 38 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<38f> a first primer having at least a part of a base sequence of the 757 to 1186th base sequence in the base sequence of SEQ ID NO: 38 or a complementary sequence thereof, and 1187 to 1 in the base sequence of SEQ ID NO: 38 A combination of second primers having at least a part of the 1773 base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (38) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<38a ′> At least a part of the 1-756th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 38, or an equivalent nucleotide sequence thereof, is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of the 1187th to 1773th base sequence in the base sequence of SEQ ID NO: 38, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<38b ′> At least a part of the 1st to 756th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 38 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1187th to 1773th base sequences in the base sequence of SEQ ID NO: 38, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<38c ′> At least a part of the 1st to 756th base sequence in the base sequence of SEQ ID NO: 38 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 757 to 1186th base sequence in the base sequence of SEQ ID NO: 38 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<38d '> A base sequence of at least a part of the 1st to 756th base sequence in the base sequence of SEQ ID NO: 38 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 757 to 1186th base sequence in the base sequence of SEQ ID NO: 38, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<38e ′> At least a part of the base sequence of the 757 to 1186th base sequence in the base sequence of SEQ ID NO: 38 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of the 1187th to 1773th base sequence in the base sequence of SEQ ID NO: 38, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<38f ′> A base sequence of at least a part of the 757 to 1186th base sequence in the base sequence of SEQ ID NO: 38, or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1187th to 1773th base sequences in the base sequence of SEQ ID NO: 38, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <38a> to <38f> and <38a ′> to <38f ′>, “the 1st to 756th base sequence in the base sequence of SEQ ID NO: 38” is “the 1st to 756th base in the base sequence of SEQ ID NO: 98” In “Sequence”, “the 1187th to 1773th base sequence in the base sequence of SEQ ID NO: 38” may be replaced with “the 757th to 1340th base sequence in the base sequence of SEQ ID NO: 98”, respectively.

  In addition to the combination of the four primers <38a ′> to <38f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <38a '> to <38f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <38a '> to <38b'> and any of <38c '> to <38f'>.

<39> Primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (39) In the base sequence of SEQ ID NO: 39, the insertion region of mPing is the nucleotide residues from 690 to 1119 (FIG. 39). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (39) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of the polymorphism (39), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations) PCR primer set and LAMP primer set are preferred: LAMP primer set using 4 primers):
<39a> a first primer having at least a partial base sequence of the 1st to 689th base sequences in the base sequence of SEQ ID NO: 39 or an equivalent base sequence thereof, and the 1120th to 1809th base sequences of the base sequence of SEQ ID NO: 39 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<39b> a first primer having at least a partial base sequence of the 1st to 689th base sequences in the base sequence of SEQ ID NO: 39 or a complementary sequence thereof, and 1120 in the base sequence of SEQ ID NO: 39 A combination of second primers having at least a part of the base sequence of the 1809th base sequence or an equivalent base sequence thereof;
<39c> a first primer having at least a partial base sequence of the 1st to 689th base sequences in the base sequence of SEQ ID NO: 39 or an equivalent base sequence thereof, and the 690th to 1119th positions in the base sequence of SEQ ID NO: 39 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<39d> a first primer having at least a partial base sequence of the 1st to 689th base sequences in the base sequence of SEQ ID NO: 39 or a complementary sequence thereof, and 690 in the base sequence of SEQ ID NO: 39 A combination of second primers having at least a part of the base sequence of the 1119th base sequence or an equivalent base sequence thereof;
<39e> a first primer having at least a part of a base sequence of 690 to 1119th in the base sequence of SEQ ID NO: 39 or an equivalent base sequence thereof, and 1120 to 1809th in the base sequence of SEQ ID NO: 39 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<39f> a first primer having at least a partial base sequence of the 690th to 1119th base sequences in the base sequence of SEQ ID NO: 39 or a complementary sequence thereof, and 1120 in the base sequence of SEQ ID NO: 39 A combination of second primers having at least a part of the 1809th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (39) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<39a ′> A base sequence of at least a part of the 1st to 689th base sequences in the base sequence of SEQ ID NO: 39 or an equivalent base sequence thereof as an F1c region or F2 region in FIP or as an F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1120 to 1809 in the base sequence of SEQ ID NO: 39, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<39b ′> A base sequence of at least a part of the 1st to 689th base sequences in the base sequence of SEQ ID NO: 39 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1120 to 1809th base sequences in the base sequence of SEQ ID NO: 39 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<39c ′> A base sequence of at least a part of the 1st to 689th base sequences in the base sequence of SEQ ID NO: 39 or an equivalent base sequence thereof as an F1c region or F2 region in FIP or as an F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 690 to 1119 in the base sequence of SEQ ID NO: 4 or a complementary sequence of its equivalent base sequence in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<39d ′> At least a part of the 1st to 689th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 39, or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 690 to 1119th base sequence in the base sequence of SEQ ID NO: 39, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<39e ′> At least a part of the base sequence of the 690th to 1119th base sequences in the base sequence of SEQ ID NO: 39 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1120 to 1809 in the base sequence of SEQ ID NO: 39, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<39f ′> A base sequence of at least a part of the 690th to 1119th base sequences in the base sequence of SEQ ID NO: 39, or a complementary sequence equivalent thereto, as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1120 to 1809th base sequences in the base sequence of SEQ ID NO: 39 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <39a> to <39f> and <39a ′> to <39f ′>, “the 1st to 689th base sequence in the base sequence of SEQ ID NO: 39” is “the 1st to 689th base in the base sequence of SEQ ID NO: 99” In the “sequence”, “the 1120th to 1809th base sequence in the base sequence of SEQ ID NO: 39” may be replaced with “the 690th to 1376th base sequence in the base sequence of SEQ ID NO: 99”, respectively.

  In addition to the four primer combinations <39a ′> to <39f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <39a ′> to <39f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <39a '> to <39b'> and any of <39c '> to <39f'>.

<40> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (40);
In the base sequence of SEQ ID NO: 40, the mPing insertion region is a region consisting of nucleotide residues 734 to 1163 (FIG. 40). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (40) mPing, the first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (40) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<40a> a first primer having at least a partial base sequence of the 1-733th base sequence in the base sequence of SEQ ID NO: 40 or an equivalent base sequence thereof, and the 1164th-1972th positions in the base sequence of SEQ ID NO: 40 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<40b> a first primer having at least a partial base sequence of the 1st to 733th base sequences in the base sequence of SEQ ID NO: 40 or a complementary sequence thereof, and 1164 to 164 in the base sequence of SEQ ID NO: 40 A second primer combination having at least a part of the base sequence of the 1972th base sequence or an equivalent base sequence thereof;
<40c> a first primer having at least a part of the 1-733th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 40 or an equivalent nucleotide sequence thereof, and the 734-1163rd nucleotide in the nucleotide sequence of SEQ ID NO: 40 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<40d> a first primer having at least a partial base sequence of the 1st to 733th base sequence in the base sequence of SEQ ID NO: 40 or a complementary sequence thereof, and 734 to in the base sequence of SEQ ID NO: 40 A combination of second primers having at least a part of the base sequence of the 1163rd base sequence or an equivalent base sequence thereof;
<40e> a first primer having at least a partial base sequence of the 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 40 or an equivalent base sequence thereof, and the 1164th to 1972th positions in the base sequence of SEQ ID NO: 40 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<40f> a first primer having at least a partial base sequence of the 734th to 1163rd base sequence in the base sequence of SEQ ID NO: 40 or a complementary sequence thereof, and 1164 to 164 in the base sequence of SEQ ID NO: 40 A combination of second primers having at least a part of the base sequence of the 1972th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (40) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<40a ′> At least a part of the 1-733th base sequence in the base sequence of SEQ ID NO: 40, or an equivalent base sequence thereof, as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of the 1164th to 1972th base sequence in the base sequence of SEQ ID NO: 40, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<40b ′> At least a part of the 1-733th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 40 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1164th to 1972th base sequence in the base sequence of SEQ ID NO: 40, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<40c ′> At least a part of the 1-733th base sequence in the base sequence of SEQ ID NO: 40 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 40 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<40d ′> At least a part of the 1-733th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 40 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 734 to 1163rd base sequence in the base sequence of SEQ ID NO: 40, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<40e ′> The base sequence of at least a part of the 734th to 1163rd base sequences in the base sequence of SEQ ID NO: 40 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of the 1164th to 1972th base sequence in the base sequence of SEQ ID NO: 40, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<40f ′> A base sequence of at least a part of the 734th to 1163rd base sequences in the base sequence of SEQ ID NO: 40 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1164th to 1972th base sequence in the base sequence of SEQ ID NO: 40, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <40a> to <40f> and <40a ′> to <40f ′>, “the 1st to 733th base sequence in the base sequence of SEQ ID NO: 40” is “the 1st to 733th base in the base sequence of SEQ ID NO: 100” In the “sequence”, “the 1164th to 1972th base sequence in the base sequence of SEQ ID NO: 40” may be replaced with “the 734th to 1539th base sequence in the base sequence of SEQ ID NO: 100”, respectively.

  In addition to the combination of the four primers <40a ′> to <40f ′>, two loop primers (LF and LB) may be used in combination.

  The combination of the four primers <40a '> to <40f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <40a '> to <40b'> and any of <40c '> to <40f'>.

<41> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (41) In the base sequence of SEQ ID NO: 41, the insertion region of mPing is the 730th to 1159th nucleotide residues (FIG. 41). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (41) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (41) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<41a> a first primer having at least a partial base sequence of the 1st to 729th base sequence in the base sequence of SEQ ID NO: 41 or an equivalent base sequence thereof, and the 1160th to 1853rd positions in the base sequence of SEQ ID NO: 41 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<41b> a first primer having at least a partial base sequence of the 1st to 729th base sequence in the base sequence of SEQ ID NO: 41 or a complementary sequence thereof, and 1160 in the base sequence of SEQ ID NO: 41 A combination of second primers having at least a part of the base sequence of the 1853rd base sequence or an equivalent base sequence thereof;
<41c> a first primer having at least a partial base sequence of the 1st to 729th base sequence in the base sequence of SEQ ID NO: 41 or an equivalent base sequence thereof, and the 730th to 1159th positions in the base sequence of SEQ ID NO: 41 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<41d> a first primer having at least a partial base sequence of the 1st to 729th base sequences in the base sequence of SEQ ID NO: 41 or a complementary sequence thereof, and 730 to 30 in the base sequence of SEQ ID NO: 41 A combination of second primers having at least a part of the base sequence of the 1159th base sequence or an equivalent base sequence thereof;
<41e> a first primer having at least a partial base sequence of the 730 to 1159th base sequence in the base sequence of SEQ ID NO: 41 or an equivalent base sequence thereof, and the 1160 to 1853rd positions in the base sequence of SEQ ID NO: 41 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<41f> a first primer having at least a partial base sequence of the 730 to 1159th base sequence in the base sequence of SEQ ID NO: 41 or a complementary sequence thereof, and 1160 in the base sequence of SEQ ID NO: 41 A combination of second primers having at least a part of the base sequence of the 1853rd base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (41) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<41a ′> At least a part of the 1-729th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 41 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1160 to 1853 in the base sequence of SEQ ID NO: 41, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<41b ′> A base sequence of at least a part of the 1st to 729th base sequence in the base sequence of SEQ ID NO: 41 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 1160 to 1853 in the base sequence of SEQ ID NO: 41, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<41c ′> At least a part of the 1st to 729th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 41 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 730 to 1159 in the base sequence of SEQ ID NO: 41, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<41d ′> At least a part of the 1st to 729th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 41 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 730 to 1159 in the base sequence of SEQ ID NO: 41, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<41e ′> A base sequence of at least a part of the 730th to 1159th base sequences in the base sequence of SEQ ID NO: 41 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1160 to 1853 in the base sequence of SEQ ID NO: 41, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<41f ′> A base sequence of at least a part of the 730th to 1159th base sequences in the base sequence of SEQ ID NO: 41 or a complementary sequence of the equivalent base sequence is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of 1160 to 1853 in the base sequence of SEQ ID NO: 41, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <41a> to <41f> and <41a ′> to <41f ′>, “the 1st to 729th base sequence in the base sequence of SEQ ID NO: 41” is “1st to 729th base in the base sequence of SEQ ID NO: 101” In the “sequence”, “the 1160th to 1853rd base sequence in the base sequence of SEQ ID NO: 41” may be replaced with “the 730th to 1420th base sequence in the base sequence of SEQ ID NO: 101”.

  In addition to the four primer combinations <41a ′> to <41f ′>, two loop primers (LF and LB) may be further used in combination.

  The four primer combinations <41a '> to <41f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <41a '> to <41b'> and any of <41c '> to <41f'>.

<42> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (42);
In the base sequence of SEQ ID NO: 42, the mPing insertion region is a region consisting of nucleotide residues 706 to 1135 (FIG. 42). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (42) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of the polymorphism (42), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<42a> a first primer having at least a part of the 1-705th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 42 or an equivalent nucleotide sequence thereof, and the 1136-1874th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 42 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<42b> a first primer having at least a partial base sequence of the 1st to 705th base sequences in the base sequence of SEQ ID NO: 42 or a complementary sequence thereof, and 1136 to 1 in the base sequence of SEQ ID NO: 42 A combination of second primers having at least a part of the base sequence of the 1874th base sequence or an equivalent base sequence thereof;
<42c> a first primer having at least a part of the 1-705th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 42 or an equivalent nucleotide sequence thereof, and the 706-1135th nucleotides in the nucleotide sequence of SEQ ID NO: 42 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<42d> a first primer having at least a partial base sequence of the 1st to 705th base sequences in the base sequence of SEQ ID NO: 42 or a complementary sequence thereof, and 706 to 706 in the base sequence of SEQ ID NO: 42 A combination of second primers having at least a part of the base sequence of the 1135th base sequence or an equivalent base sequence thereof;
<42e> a first primer having at least a partial base sequence of the 706 to 1135th base sequence in the base sequence of SEQ ID NO: 42 or an equivalent base sequence thereof, and the 1136 to 1874th positions of the base sequence of SEQ ID NO: 42 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<42f> a first primer having at least a partial base sequence of the 706 to 1135th base sequence in the base sequence of SEQ ID NO: 42 or a complementary sequence of the equivalent base sequence, and 1136 of the base sequence of SEQ ID NO: 42 A combination of second primers having at least a part of the base sequence of 1874th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (42) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<42a ′> At least a part of the 1st to 705th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 42 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably The first primer having (as F2 region in FIP), and at least a part of the base sequence of 1136 to 1874th base sequence in the base sequence of SEQ ID NO: 42 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<42b ′> At least a part of the 1st to 705th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 42, or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 1136 to 1874th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 42 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<42c ′> At least a part of the 1st to 705th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 42 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 706 to 1135 in the base sequence of SEQ ID NO: 42, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<42d '> A base sequence of at least a part of the 1st to 705th base sequences in the base sequence of SEQ ID NO: 42 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the nucleotide sequence of the 706 to 1135th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 42 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<42e '> The base sequence of at least a part of the 706 to 1135th base sequences in the base sequence of SEQ ID NO: 42 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably The first primer having (as F2 region in FIP), and at least a part of the base sequence of 1136 to 1874th base sequence in the base sequence of SEQ ID NO: 42 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<42f ′> A base sequence of at least a part of the 706th to 1135th base sequences in the base sequence of SEQ ID NO: 42 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the 1136 to 1874th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 42 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <42a> to <42f> and <42a ′> to <42f ′>, “the 1st to 705th base sequence in the base sequence of SEQ ID NO: 42” is “the 1st to 705th base in the base sequence of SEQ ID NO: 102” In the “sequence”, “the 1136th to 1874th base sequence in the base sequence of SEQ ID NO: 42” may be replaced with “the 706th to 1441th base sequence in the base sequence of SEQ ID NO: 102”, respectively.

  In addition to the four primer combinations <42a ′> to <42f ′>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <42a '> to <42f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <42a '> to <42b'> and any of <42c '> to <42f'>.

<43> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (43) In the nucleotide sequence of SEQ ID NO: 43, the insertion region of mPing is the nucleotide residues 604 to 1033 (FIG. 43). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (43) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

As a primer set for mPing of the polymorphism (43), a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<43a> a first primer having at least a part of the base sequence of 1 to 603 in the base sequence of SEQ ID NO: 43 or an equivalent base sequence thereof, and the 1034 to 1784 th of base sequence of SEQ ID NO: 43 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<43b> a first primer having at least a partial base sequence of the 1st to 603rd base sequences in the base sequence of SEQ ID NO: 43 or a complementary sequence thereof, and 1034 to 10 in the base sequence of SEQ ID NO: 43 A combination of second primers having at least a part of the 1784th base sequence or an equivalent base sequence thereof;
<43c> a first primer having at least a part of the base sequence of 1 to 603 in the base sequence of SEQ ID NO: 43 or an equivalent base sequence thereof, and the 604th to 1033rd positions of the base sequence of SEQ ID NO: 43 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<43d> a first primer having at least a partial base sequence of the 1st to 603rd base sequences in the base sequence of SEQ ID NO: 43 or a complementary sequence thereof, and 604 to 604 in the base sequence of SEQ ID NO: 43 A combination of second primers having at least a part of the base sequence of the 1033rd base sequence or an equivalent base sequence thereof;
<43e> a first primer having at least a part of the base sequence of 604 to 1033 in the base sequence of SEQ ID NO: 43 or an equivalent base sequence thereof, and the 1034 to 1784th of the base sequence of SEQ ID NO: 43 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<43f> a first primer having at least a partial base sequence of the 604th to 1033rd base sequences in the base sequence of SEQ ID NO: 43 or a complementary sequence thereof, and 1034 to 1034 in the base sequence of SEQ ID NO: 43 A combination of second primers having at least part of the 1784th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (43) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<43a ′> A base sequence of at least a part of the 1st to 603rd base sequences in the base sequence of SEQ ID NO: 43 or an equivalent base sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1034 to 1784 in the base sequence of SEQ ID NO: 43, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<43b ′> A base sequence of at least a part of the 1st to 603rd base sequence in the base sequence of SEQ ID NO: 43 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1034 to 1784th base sequence in the base sequence of SEQ ID NO: 43 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<43c ′> A base sequence of at least a part of the 1st to 603rd base sequence in the base sequence of SEQ ID NO: 43 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 604 to 1033 in the base sequence of SEQ ID NO: 43, or a complementary sequence thereof, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<43d ′> A base sequence of at least a part of the 1st to 603rd base sequence in the base sequence of SEQ ID NO: 43 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 604th to 1033rd base sequences in the base sequence of SEQ ID NO: 43 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<43e ′> At least a part of the nucleotide sequence of the 604th to 1033rd nucleotide sequences in the nucleotide sequence of SEQ ID NO: 43 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1034 to 1784 in the base sequence of SEQ ID NO: 43, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<43f ′> A base sequence of at least a part of the 604th to 1033rd base sequences in the base sequence of SEQ ID NO: 43 or a complementary sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1034 to 1784th base sequence in the base sequence of SEQ ID NO: 43 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <43a> to <43f> and <43a ′> to <43f ′>, “the 1st to 603rd base sequence in the base sequence of SEQ ID NO: 43” is “the 1st to 603rd base in the base sequence of SEQ ID NO: 103” In the “sequence”, “the 1034th to 1784th base sequence in the base sequence of SEQ ID NO: 43” may be replaced with “the 604th to 1351st base sequence in the base sequence of SEQ ID NO: 103”, respectively.

  In addition to the four primer combinations <43a ′> to <43f ′>, two loop primers (LF and LB) may be used in combination.

  The combination of the four primers <43a '> to <43f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <43a '> to <43b'> and any of <43c '> to <43f'>.

<44> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (44);
In the base sequence of SEQ ID NO: 44, the mPing insertion region is a region consisting of nucleotide residues 728 to 1157 (FIG. 44). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (44) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (44) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets (eg, at least, including such primer combinations). PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<44a> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 44 or an equivalent base sequence thereof, and the 1158th to 1976th positions in the base sequence of SEQ ID NO: 44 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<44b> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 44 or a complementary sequence thereof, and 1158 to the base sequence of SEQ ID NO: 44 A second primer combination having at least a part of the base sequence of the 1976th base sequence or an equivalent base sequence thereof;
<44c> a first primer having at least a part of the 1-727th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 44 or an equivalent nucleotide sequence thereof, and the 728-1157th nucleotide in the nucleotide sequence of SEQ ID NO: 44 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<44d> a first primer having at least a partial base sequence of the 1st to 727th base sequence in the base sequence of SEQ ID NO: 44 or a complementary sequence thereof, and 728 to in the base sequence of SEQ ID NO: 44 A combination of second primers having at least a part of the base sequence of the 1157th base sequence or an equivalent base sequence thereof;
<44e> a first primer having at least a partial base sequence of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 44 or an equivalent base sequence thereof, and the 1158th to 1976th positions in the base sequence of SEQ ID NO: 44 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<44f> a first primer having at least a partial base sequence of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 44 or a complementary sequence thereof, and 1158-in the base sequence of SEQ ID NO: 44 A combination of second primers having at least a part of the base sequence of the 1976th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (44) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<44a ′> At least a part of the 1-727th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 44 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1158 to 1976 in the base sequence of SEQ ID NO: 44, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<44b ′> A base sequence of at least a part of the 1st to 727th base sequences in the base sequence of SEQ ID NO: 44 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1158th to 1976th base sequence in the base sequence of SEQ ID NO: 44 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<44c ′> At least a part of the 1-727th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 44 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP, or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 728 to 1157th in the base sequence of SEQ ID NO: 44, or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<44d ′> At least a part of the 1-727th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 44 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the nucleotide sequence of the 728 to 1157th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 44 or an equivalent nucleotide sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<44e ′> A base sequence of at least a part of the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 44 or an equivalent base sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 1158 to 1976 in the base sequence of SEQ ID NO: 44, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<44f ′> At least a part of the base sequence from the 728 to 1157th base sequence in the base sequence of SEQ ID NO: 44 or a complementary sequence equivalent thereto is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1158th to 1976th base sequence in the base sequence of SEQ ID NO: 44 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <44a> to <44f> and <44a ′> to <44f ′>, “the 1st to 727th base sequence in the base sequence of SEQ ID NO: 44” is “the 1st to 727th base in the base sequence of SEQ ID NO: 104” In the “sequence”, “the 1158th to 1976th base sequence in the base sequence of SEQ ID NO: 44” may be replaced with “the 728th to 1543th base sequence in the base sequence of SEQ ID NO: 104”, respectively.

  In addition to the combination of the four primers <44a ′> to <44f ′>, two loop primers (LF and LB) may be further used in combination.

  The combinations of the four primers <44a '> to <44f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <44a '> to <44b'> and any of <44c '> to <44f'>.

<45> Primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (45) In the base sequence of SEQ ID NO: 45, the insertion region of mPing is the nucleotide residues 735 to 1164 (FIG. 45). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphic (45) mPing, the first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (45) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<45a> a first primer having at least a partial base sequence of the 1-734th base sequence in the base sequence of SEQ ID NO: 45 or an equivalent base sequence thereof, and 1165-1887th positions in the base sequence of SEQ ID NO: 45 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<45b> a first primer having at least a partial base sequence of the 1st to 734th base sequences in the base sequence of SEQ ID NO: 45 or a complementary sequence of the equivalent base sequence, and 1165 in the base sequence of SEQ ID NO: 45 A combination of second primers having at least a part of the base sequence of the 1887th base sequence or an equivalent base sequence thereof;
<45c> a first primer having at least a partial base sequence of the 1st to 734th base sequence in the base sequence of SEQ ID NO: 45 or an equivalent base sequence thereof, and the 735th to 1164th bases of the base sequence of SEQ ID NO: 45 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<45d> a first primer having at least a partial base sequence of the 1st to 734th base sequences in the base sequence of SEQ ID NO: 45 or a complementary sequence thereof, and 735 in the base sequence of SEQ ID NO: 45 A combination of second primers having at least a part of the base sequence of the 1164th base sequence or an equivalent base sequence thereof;
<45e> a first primer having at least a partial base sequence of the 735th to 1164th base sequence in the base sequence of SEQ ID NO: 45 or an equivalent base sequence thereof, and the 1165th to 1887th positions of the base sequence of SEQ ID NO: 45 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<45f> a first primer having at least a partial base sequence of the 735th to 1164th base sequences in the base sequence of SEQ ID NO: 45 or a complementary sequence thereof, and 1165 in the base sequence of SEQ ID NO: 45 A combination of second primers having at least a part of the base sequence of the 1887th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (45) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<45a ′> At least a part of the 1-734th base sequence in the base sequence of SEQ ID NO: 45 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1165 to 1887 in the base sequence of SEQ ID NO: 45 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<45b ′> A base sequence of at least a part of the 1st to 734th base sequence in the base sequence of SEQ ID NO: 45 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1165th to 1887th base sequences in the base sequence of SEQ ID NO: 45 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<45c ′> At least a part of the 1-734th base sequence in the base sequence of SEQ ID NO: 45 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 735 to 1164 in the base sequence of SEQ ID NO: 4 or a complementary sequence of the equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<45d ′> At least a part of the 1st to 734th base sequence in the base sequence of SEQ ID NO: 45, or a complementary sequence equivalent thereto, as F1c region or F2 region in FIP, or as F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 735th to 1164th base sequences in the base sequence of SEQ ID NO: 45, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<45e ′> The base sequence of at least a part of the 735th to 1164th base sequences in the base sequence of SEQ ID NO: 45 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1165 to 1887 in the base sequence of SEQ ID NO: 45 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<45f '> A base sequence of at least a part of the 735th to 1164th base sequences in the base sequence of SEQ ID NO: 45 or a complementary sequence of the equivalent base sequence is used as the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1165th to 1887th base sequences in the base sequence of SEQ ID NO: 45 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <45a> to <45f> and <45a ′> to <45f ′>, “the 1st to 734th base sequence in the base sequence of SEQ ID NO: 45” is “the 1st to 734th base in the base sequence of SEQ ID NO: 105” In the “sequence”, the “1165th to 1887th base sequence in the base sequence of SEQ ID NO: 45” may be replaced with “the 735th to 1454th base sequence in the base sequence of SEQ ID NO: 105”.

  In addition to the four primer combinations <45a ′> to <45f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <45a '> to <45f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <45a '> to <45b'> and any of <45c '> to <45f'>.

<46> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (46);
In the base sequence of SEQ ID NO: 46, the mPing insertion region is a region consisting of nucleotide residues 654 to 1083 (FIG. 46). Accordingly, as two or more primers for detecting the presence or absence of insertion of mPing of the polymorphism (46), a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (46) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<46a> a first primer having at least a partial base sequence of the 1st to 653rd base sequences in the base sequence of SEQ ID NO: 46 or an equivalent base sequence thereof, and the 1084th to 1672th positions of the base sequence of SEQ ID NO: 46 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<46b> a first primer having at least a partial base sequence of the 1st to 653rd base sequences in the base sequence of SEQ ID NO: 46 or a complementary sequence thereof, and 1084 to 1084 in the base sequence of SEQ ID NO: 46 A combination of second primers having at least a part of the base sequence of the 1672th base sequence or an equivalent base sequence thereof;
<36c> a first primer having at least a part of the 1st to 653rd base sequences in the base sequence of SEQ ID NO: 36 or an equivalent base sequence thereof, and the 654th to 1083th positions in the base sequence of SEQ ID NO: 46 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<46d> a first primer having at least a partial base sequence of the 1st to 653rd base sequences in the base sequence of SEQ ID NO: 46 or a complementary sequence thereof, and 654 in the base sequence of SEQ ID NO: 46 A second primer combination having at least a part of the base sequence of the 1083rd base sequence or an equivalent base sequence thereof;
<46e> a first primer having at least a partial base sequence of the 654th to 1083rd base sequences in the base sequence of SEQ ID NO: 46 or an equivalent base sequence thereof, and the 1084th to 1672th positions in the base sequence of SEQ ID NO: 46 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<46f> a first primer having at least a part of a base sequence of the 654th to 1083rd base sequences in the base sequence of SEQ ID NO: 46 or a complementary sequence of the equivalent base sequence, and 1084 to 1084 in the base sequence of SEQ ID NO: 46 A combination of second primers having at least a part of the base sequence of the 1672th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphism (46) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<46a ′> The base sequence of at least a part of the 1st to 653rd base sequences in the base sequence of SEQ ID NO: 46 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1084 to 1672th base sequence in the base sequence of SEQ ID NO: 46, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<46b ′> A base sequence of at least a part of the 1st to 653rd base sequences in the base sequence of SEQ ID NO: 46, or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1084th to 1672th base sequences in the base sequence of SEQ ID NO: 46, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<46c ′> A base sequence of at least a part of the 1st to 653rd base sequences in the base sequence of SEQ ID NO: 46 or an equivalent base sequence thereof as an F1c region or F2 region in FIP or as an F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 654 to 1083 in the base sequence of SEQ ID NO: 46, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<46d ′> A base sequence of at least a portion of the 1st to 653rd base sequences in the base sequence of SEQ ID NO: 46, or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 654th to 1083rd base sequences in the base sequence of SEQ ID NO: 36, or an equivalent base sequence thereof, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<46e ′> At least a part of the base sequence of the 654th to 1083th base sequences in the base sequence of SEQ ID NO: 46 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 1084 to 1672th base sequence in the base sequence of SEQ ID NO: 46, or a complementary sequence equivalent thereto, in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<46f ′> A base sequence of at least a part of the 654th to 1083rd base sequences in the base sequence of SEQ ID NO: 46 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and the base sequence of at least a part of the 1084th to 1672th base sequences in the base sequence of SEQ ID NO: 46, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <46a> to <46f> and <46a ′> to <46f ′>, “the 1st to 653rd base sequence in the base sequence of SEQ ID NO: 46” is “the 1st to 653rd base in the base sequence of SEQ ID NO: 106” In the “sequence”, “the 1084th to 1672th base sequence in the base sequence of SEQ ID NO: 46” may be replaced with “the 654th to 1316th base sequence in the base sequence of SEQ ID NO: 106”, respectively.

  In addition to the combination of the four primers <46a ′> to <46f ′>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <46a '> to <46f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <46a '> to <46b'> and any of <46c '> to <46f'>.

<47> Primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (47) In the base sequence of SEQ ID NO: 47, the insertion region of mPing is the 727 to 1156th nucleotide residue (FIG. 47). Therefore, as two or more primers for detecting the presence or absence of insertion of mPing of the polymorphism (47), a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (47) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such a combination of primers (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<47a> a first primer having at least a partial base sequence of the 1st to 726th base sequence in the base sequence of SEQ ID NO: 47 or an equivalent base sequence thereof, and the 1156th to 1869th positions of the base sequence of SEQ ID NO: 47 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<47b> a first primer having at least a partial base sequence of the 1st to 726th base sequences in the base sequence of SEQ ID NO: 47 or a complementary sequence thereof, and 1156 to 1 in the base sequence of SEQ ID NO: 47 A combination of second primers having at least a part of the base sequence of the 1869th base sequence or an equivalent base sequence thereof;
<47c> a first primer having at least a partial base sequence of the 1st to 726th base sequence in the base sequence of SEQ ID NO: 47 or an equivalent base sequence thereof, and the 727 to 1156th base sequence of the base sequence of SEQ ID NO: 47 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<47d> a first primer having at least a partial base sequence of the 1st to 726th base sequences in the base sequence of SEQ ID NO: 47 or a complementary sequence of the equivalent base sequence, and 727 to in the base sequence of SEQ ID NO: 37 A combination of second primers having at least a part of the base sequence of the 1156th base sequence or an equivalent base sequence thereof;
<47e> a first primer having at least a partial base sequence of the 727 to 1156th base sequence in the base sequence of SEQ ID NO: 47 or an equivalent base sequence thereof, and the 1156 to 1869th positions of the base sequence of SEQ ID NO: 47 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<47f> a first primer having at least a partial base sequence of the 727 to 1156th base sequence in the base sequence of SEQ ID NO: 47 or a complementary sequence of the equivalent base sequence, and 1156 of the base sequence of SEQ ID NO: 47 A combination of second primers having at least a part of the 1869th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (47) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<47a ′> A base sequence of at least a part of the 1st to 726th base sequence in the base sequence of SEQ ID NO: 47 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1156 to 1869 in the base sequence of SEQ ID NO: 47 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<47b ′> A base sequence of at least a part of the 1st to 726th base sequences in the base sequence of SEQ ID NO: 47 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1156 to 1869th base sequence in the base sequence of SEQ ID NO: 47, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<47c ′> At least a part of the 1st to 726th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 47 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of the 727 to 1156th base sequence in the base sequence of SEQ ID NO: 47 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<47d ′> A base sequence of at least a part of the 1st to 726th base sequences in the base sequence of SEQ ID NO: 47 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 727 to 1156th base sequence in the base sequence of SEQ ID NO: 47, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<47e ′> At least a part of the 727 to 1156th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 47 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as an F2 region in FIP), and at least a part of the base sequence of 1156 to 1869 in the base sequence of SEQ ID NO: 47 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<47f ′> At least a part of the 727 to 1156th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 47 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP), and at least a part of the base sequence of the 1156 to 1869th base sequence in the base sequence of SEQ ID NO: 47, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <47a> to <47f> and <47a ′> to <47f ′>, “the 1st to 726th base sequence in the base sequence of SEQ ID NO: 47” is “the 1st to 726th base in the base sequence of SEQ ID NO: 107” In the “sequence”, “the 1156th to 1869th base sequence in the base sequence of SEQ ID NO: 47” may be replaced with “the 727th to 1437th base sequence in the base sequence of SEQ ID NO: 107”, respectively.

  In addition to the four primer combinations <47a '> to <47f'>, two loop primers (LF and LB) may be used in combination.

  The four primer combinations <47a ′> to <47f ′> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <47a '> to <47b'> and any of <47c '> to <47f'>.

<48> a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (48);
In the base sequence of SEQ ID NO: 48, the insertion region of mPing is a region consisting of the 627th to 1056th nucleotide residues (FIG. 48). Therefore, as two or more primers for detecting the presence or absence of insertion of the polymorphism (48) mPing, a first primer having a base sequence corresponding to the upstream site (sense strand or antisense strand) of this region, and Combination of second primers having a base sequence corresponding to the downstream site (antisense strand or sense strand) of this region (mPing insertion by obtaining amplification products having different sizes depending on whether mPing is inserted or not) A first primer having a base sequence corresponding to an upstream site (sense strand or antisense strand) of this region, or a downstream site (antisense strand or sense strand) of this region, And a second primer combination having a base sequence corresponding to this region (sense strand or antisense strand) ( Combinations can be used) of a primer capable of detecting the presence or absence of insertion of mPing the presence or absence of a width (see FIG. 50).

The primer set for the polymorphism (48) mPing includes a combination of primers composed of the following primer pairs (eg, primers for PCR), and three or more primer sets including such primer combinations (eg, at least PCR primer set and LAMP primer set are preferred: a LAMP primer set using four primers):
<48a> a first primer having at least a partial base sequence of the 1st to 626th base sequence in the base sequence of SEQ ID NO: 48 or an equivalent base sequence thereof, and the 1057th to 1694th positions of the base sequence of SEQ ID NO: 48 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<48b> a first primer having at least a partial base sequence of the 1st to 626th base sequences in the base sequence of SEQ ID NO: 48 or a complementary sequence of the equivalent base sequence, and 1057 to 1057 in the base sequence of SEQ ID NO: 48 A second primer combination having at least a part of the base sequence of the 1694th base sequence or an equivalent base sequence thereof;
<48c> a first primer having at least a partial base sequence of the 1st to 626th base sequence in the base sequence of SEQ ID NO: 48 or an equivalent base sequence thereof, and the 627th to 1056th positions of the base sequence of SEQ ID NO: 48 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<48d> a first primer having at least a partial base sequence of the 1st to 626th base sequences in the base sequence of SEQ ID NO: 48 or a complementary sequence thereof, and 627 to in the base sequence of SEQ ID NO: 48 A second primer combination having at least a part of the base sequence of the 1056th base sequence or an equivalent base sequence thereof;
<48e> a first primer having at least a partial base sequence of the 627th to 1056th base sequences in the base sequence of SEQ ID NO: 48 or an equivalent base sequence thereof, and the 1057th to 1694th positions in the base sequence of SEQ ID NO: 48 A combination of second primers having at least a part of the base sequence or a sequence complementary to the equivalent base sequence;
<48f> a first primer having at least a partial base sequence of the 627th to 1056th base sequences in the base sequence of SEQ ID NO: 48 or a complementary sequence of the equivalent base sequence, and 1057 to 1057 in the base sequence of SEQ ID NO: 48 A combination of second primers having at least a part of the base sequence of the 1694th base sequence or an equivalent base sequence thereof.

The LAMP primer set capable of detecting the presence or absence of insertion of the polymorphic (48) mPing is composed of a combination of four primers (FIP, F3, BIP, B3) including at least the following two primers:
<48a ′> At least a part of the 1st to 626th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 48 or an equivalent nucleotide sequence thereof as F1c region or F2 region in FIP or as F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the nucleotide sequence of the 1057 to 1694th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 48 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<48b ′> At least a part of the 1st to 626th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 48 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1057th to 1694th base sequences in the base sequence of SEQ ID NO: 48 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<48c ′> At least a part of the 1-626th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 48 or an equivalent nucleotide sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the base sequence of 627 to 1056 in the base sequence of SEQ ID NO: 48, or a complementary sequence of its equivalent base sequence, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<48d ′> At least a part of the 1st to 626th nucleotide sequences in the nucleotide sequence of SEQ ID NO: 48 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 627th to 1056th base sequences in the base sequence of SEQ ID NO: 48, or an equivalent base sequence thereof, A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<48e ′> At least a part of the 627th to 1056th base sequences in the base sequence of SEQ ID NO: 48 or an equivalent base sequence thereof is used as the F1c region or F2 region in FIP, or as the F3 region (preferably A first primer having (as F2 region in FIP), and at least a part of the nucleotide sequence of the 1057 to 1694th nucleotide sequence in the nucleotide sequence of SEQ ID NO: 48 or a complementary sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP);
<48f ′> A base sequence of at least a part of the 627th to 1056th base sequences in the base sequence of SEQ ID NO: 48 or a complementary sequence thereof equivalent to the F1c region or F2 region in FIP, or as the F3 region The first primer having (preferably as the F2 region in FIP) and at least a part of the base sequence of the 1057th to 1694th base sequences in the base sequence of SEQ ID NO: 48 or an equivalent base sequence thereof in BIP A combination of four primers comprising a second primer having as a B1c or B2 region or as a B3 region (preferably as a B2 region in BIP).

  In <48a> to <48f> and <48a ′> to <48f ′>, “the 1st to 626th base sequence in the base sequence of SEQ ID NO: 48” is “1st to 626th base in the base sequence of SEQ ID NO: 108” In the “sequence”, “the 1057th to 1694th base sequence in the base sequence of SEQ ID NO: 48” may be replaced with “the 627th to 1261st base sequence in the base sequence of SEQ ID NO: 108”, respectively.

  In addition to the four primer combinations <48a '> to <48f'>, two loop primers (LF and LB) may be further used in combination.

  The combination of the four primers <48a '> to <48f'> may be used alone or in combination of two or more. It is preferable to combine two or more, and it is more preferable to combine any of <48a '> to <48b'> and any of <48c '> to <48f'>.

  The term “equivalent base sequence” in the various primers constituting the primer set mentioned above means a base sequence corresponding to a variation in base sequence between rice varieties, in particular, varieties other than Nipponbare and Nipponbare. . Therefore, several base sequences equivalent to a predetermined base sequence (eg, at least a part of the base sequence of Y to Z in the base sequence of SEQ ID NO. X) are several (for example, 1 to 80, 1-70, 1-60, 1-50, 1-40, 1-30, 1-20, 1-10, preferably 1-5, more preferably 1, It may be a base sequence containing 2 or 3 base modifications (eg, substitution, deletion, insertion, addition). Such an equivalent base sequence is obvious to those skilled in the art, and by referring to a database in which gene information of rice varieties is registered, or around the insertion region of the target mPing in rice varieties. This can be determined by decoding the base sequence.

  The presence or absence of mPing insertion in polymorphism (1) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (1). Can be identified. For example, if it is confirmed that the polymorphic (1) mPing is inserted, the sample is likely to be spilled and / or tender, or may contain spill and / or tenderness Can be determined to be high. On the other hand, if it is confirmed that the polymorphism (1) mPing has not been inserted, the sample is likely to be of a variety other than the first eyebrow and the tenderness, or includes a variety other than the first eyebrow and the tenderness. It can be determined that the possibility is high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (2) confirmed the presence or absence of mPing insertion in polymorphism (2). Can be identified. For example, when it is confirmed that mPing of polymorphism (2) is inserted, there is a possibility that the sample is one or more selected from the group consisting of Kitakomachi, Haenuki, Tsugaru Roman, Sasanishiki, and Kokomochi. It can be determined that the sample is high, or that the sample is likely to contain one or more selected from the group consisting of Kitamachi, Haenuki, Tsugaru Roman, Sasanishiki, and Kokomochi. On the other hand, if it is confirmed that the polymorphic (2) mPing is not inserted, the sample is likely to be a variety other than Kitamachi, Haenuki, Tsugaru Roman, Sasanishiki and Koganemochi, or Akitakomachi, It can be determined that varieties other than Haenuki, Tsugaru Roman, Sasanishiki and Kokomochi are included.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (3) confirmed the presence or absence of mPing insertion in polymorphism (3). Can be identified. For example, if it is confirmed that the polymorphic (3) mPing is inserted, the sample should be selected from Hinohikari, Yukinko Mai, Kuma no Mori, Koko no Komochi, Hachichochimochi and Kitayukimochi Or more than one selected from Hinohikari, Yukiniko Dance, Kuma no Mori, Kaze no Komochi, Hachichochimochi and Kitayukimochi it can. On the other hand, if it is confirmed that the polymorphic (3) mPing is not inserted, the sample is not Hinohikari, Yukiniko Mai, Kuma no Mori, Kaze no Komochi, Hachichochimochi and Kitayukimochi It can be determined that it is highly likely to be a variety, or that it is highly likely to include varieties other than Hinohikari, Yukiniko Dance, Kuma-no-Mori, Kaze no Komochi, Hachichochimochi and Kitayukimochi.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (4) confirmed the presence or absence of mPing insertion in polymorphism (4). Can be identified. For example, when it is confirmed that the polymorphic (4) mPing is inserted, the sample is likely to be a nanaboshi or the sample is likely to include a nanaboshi. Can be judged. On the other hand, if it is confirmed that the polymorphic (4) mPing has not been inserted, it is highly possible that the sample is of a variety other than Nanatsubashi, or that the sample is of a type other than Nanatsuboshi. It can be determined that the possibility of inclusion is high.

  The presence or absence of mPing insertion in polymorphism (5) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (5). Can be identified. For example, when it is confirmed that the mPing of polymorphism (5) is inserted, the sample is likely to be a nanaboshi or the sample is likely to include a nanaboshi. Can be judged. On the other hand, if it is confirmed that the polymorphic (5) mPing has not been inserted, it is highly possible that the sample is of a variety other than Nanatsubashi, or that the sample is of a type other than Nanatsuboshi. It can be determined that the possibility of inclusion is high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (6) confirmed the presence or absence of mPing insertion in polymorphism (6). Can be identified. For example, when it is confirmed that the polymorphic (6) mPing is inserted, the sample is likely to be at least one selected from the group consisting of Haenuki, Kobuki Buki and Yukinko Dance. Alternatively, it can be determined that there is a high possibility that the sample contains one or more selected from the group consisting of enoki, kobuki and yukinko dance. On the other hand, if it is confirmed that the polymorphic (6) mPing has not been inserted, the sample is likely to be a variety other than Hanuki, Koukibuki, and Yukiniko Mai, or And it can be determined that there is a high possibility of including varieties other than Yukinko Mai.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (7) confirmed the presence or absence of mPing insertion in polymorphism (7). Can be identified. For example, when it is confirmed that mPing of polymorphism (7) is inserted, the sample is likely to be one or more selected from the group consisting of Kirara 397, Furukiko and Hoshinome. Alternatively, it can be determined that there is a high possibility of including one or more selected from the group consisting of Kirara 397, Furukikonko and Hoshinoyume. On the other hand, if it is confirmed that the polymorphic (7) mPing is not inserted, it is highly possible that the sample is a variety other than Kirara 397, Furukiko and Hoshino Yume, or Kirara 397 It can be judged that there is a high possibility of including varieties other than Nko and Hoshi no Yume.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (8) confirmed the presence or absence of mPing insertion in polymorphism (8). Can be identified. For example, when it is confirmed that the polymorphic (8) mPing is inserted, it is highly possible that the sample is one or more selected from the group consisting of Kirara 397, Yumepirika, and Tarotsuki, or It can be determined that there is a high possibility that the sample contains one or more selected from the group consisting of Kirara 397, Yumepirika, and Oborozuki. On the other hand, when it is confirmed that the polymorphic (8) mPing is not inserted, it is highly likely that the sample is of a variety other than Kirara 397, Yumepirika or Orotsuki, or the sample is Kirara 397, Yume It can be determined that there is a high possibility of including varieties other than Pirika and Orozuki.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (9) confirmed the presence or absence of mPing insertion in polymorphism (9). Can be identified. For example, when it is confirmed that mPing of polymorphism (9) is inserted, it is highly possible that the sample is one or more selected from the group consisting of Tatsukomochi and Kinu. Alternatively, it can be determined that there is a high possibility of including one or more kinds selected from the group consisting of masigura, tatsukomochi, and kinu. On the other hand, if it is confirmed that the polymorphism (9) mPing has not been inserted, the sample is likely to be a variety other than the true tsugachi, tatsukomochi and kinu, or the It can be determined that there is a high possibility of including varieties other than Tsukomochi and Kinu-no-Hada.

  The presence or absence of mPing insertion in polymorphism (10) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (10). Can be identified. For example, if it is confirmed that an mPing of polymorphism (10) is inserted, the sample is likely to be a good splash and / or a dancer, or the sample is a good splash and / or It can be determined that there is a high possibility of including Yukinko Dance. On the other hand, if it is confirmed that the polymorphic (10) mPing has not been inserted, the sample is likely to be of a variety other than the fragile and yukiniko dance, or the confused and yukinko It can be determined that there is a high possibility of including varieties other than Mai.

  The presence or absence of mPing insertion in polymorphism (11) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (11). Can be identified. For example, if it is confirmed that mPing of polymorphism (11) is inserted, the sample is likely to be a fragile splash and / or a dancer, or the sample is a fragile splash and / or It can be determined that there is a high possibility of including Yukinko Dance. On the other hand, if it is confirmed that the polymorphism (11) mPing has not been inserted, the sample is likely to be of a variety other than a delicate splash and / or yukinko dance, or the sample is And / or it can be determined that there is a high possibility of including varieties other than Yukinko Mai.

  The presence or absence of mPing insertion in polymorphism (12) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (12). Can be identified. For example, when it is confirmed that mPing of the polymorphism (12) is inserted, one or more selected from the group consisting of romantic, kinukari, kinusume, nikkoru, nasuhikari and akisakari with which the sample is attached It can be determined that the sample is likely to contain one or more selected from the group consisting of romance, kinukari, kinusume, nikkoru, nasuhikari and akisakari. On the other hand, when it is confirmed that the polymorphic (12) mPing is not inserted, it is highly possible that the sample is a variety other than the romantic, Kinukari, Kinusume, Nikkoru, Nasuhikari, and Akisakakari. Alternatively, it can be determined that the sample is likely to contain varieties other than romance, kinuhikari, kinusume, nikkoru, nasuhikari and akisakari.

  The presence or absence of mPing insertion in polymorphism (13) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (13). Can be identified. For example, when it is confirmed that the polymorphic (13) mPing has been inserted, it can be determined that the sample is likely to be Kinuhikari or likely to contain Kinuhikari. On the other hand, when it is confirmed that the polymorphic (13) mPing is not inserted, it can be determined that the sample is likely to be a variety other than Kinuhikari, or is likely to contain a variety other than Kinuhikari. .

  The presence or absence of mPing insertion in polymorphism (14) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (14). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (14) is inserted, it can be determined that the sample is highly likely to be a dream of Asahi or that the sample is likely to contain a dream of Asahi . On the other hand, if it is confirmed that the polymorphic (14) mPing has not been inserted, the sample is likely to be a variety other than the dream of Asahi, or the sample may include a variety other than the dream of Asahi Judgment is high.

  The presence or absence of mPing insertion in polymorphism (15) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (15). Can be identified. For example, if it is confirmed that the polymorphic (15) mPing has been inserted, the sample is from the group consisting of Yumepirika, Fukuchinko, Hoshino Yume, Kaze no Komochi, Hachichochimochi and Kitayukimochi There is a high probability that the sample will be one or more selected, or the sample may contain one or more selected from the group consisting of Yumepirika, Fukumiko, Hoshino Yume, Kaze no Komochi, Hachichochimochi and Kitayukimochi Can be determined to be high. On the other hand, if it is confirmed that the polymorphic (15) mPing is not inserted, the sample must be a variety other than Yumepirika, Fukukinko, Hoshino Yume, Kaze no Komochi, Hachichochimochi and Kitayukimochi. It can be judged that there is a high possibility, or that the sample is likely to contain varieties other than Yume Piri, Fukumiko, Hoshino Yume, Kaze no Komochi, Hachichochimochi and Kitayukimochi.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (16) confirmed the presence or absence of mPing insertion in polymorphism (16). Can be identified. For example, when it is confirmed that the mPing of polymorphism (16) is inserted, it can be determined that the sample is likely to contain cocoon or the sample is likely to contain cocoon. . On the other hand, if it is confirmed that the polymorphic (16) mPing has not been inserted, the sample is likely to be of a variety other than Fusakogane, or the sample may contain a variety other than Fusogane Judgment is high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (17) confirmed the presence or absence of mPing insertion in polymorphism (17). Can be identified. For example, when it is confirmed that the polymorphic (17) mPing is inserted, it can be determined that the sample is highly likely to be a princess or the sample is likely to contain a princess. On the other hand, when it is confirmed that the polymorphism (17) mPing is not inserted, there is a high possibility that the sample is a variety other than Tsutsuhime, or the sample may contain a breed other than Tsutsuhime. Can be determined to be high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (18) confirmed the presence or absence of mPing insertion in polymorphism (18). Can be identified. For example, when it is confirmed that the polymorphic (18) mPing is inserted, it can be determined that the sample is highly likely to be a princess or the sample is likely to contain a princess. On the other hand, when it is confirmed that the polymorphic (18) mPing is not inserted, there is a high possibility that the sample is a variety other than Tsutsuhime, or the sample may contain a breed other than Tsutsuhime. Can be determined to be high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (19) confirmed the presence or absence of mPing insertion in the polymorphism (19). Can be identified. For example, when it is confirmed that the polymorphism (19) mPing is inserted, it can be determined that the sample is highly likely to be hanaethizen, or that the sample is likely to contain hanaethizen. On the other hand, when it is confirmed that the polymorphism (19) mPing is not inserted, it is highly possible that the sample is of a variety other than Hanaechizen, or that the sample is likely to contain a variety other than Hanaechizen. Can be judged.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (20) confirmed the presence or absence of mPing insertion in polymorphism (20). Can be identified. For example, if it is confirmed that mPing of the polymorphism (20) is inserted, the sample has a dream and is likely to be one or more selected from the group consisting of Kinuhikari and Natsukihikari, or It can be determined that there is a high possibility that the sample has a dream and contains at least one selected from the group consisting of Kinuhikari and Nasuhikari. On the other hand, if it is confirmed that the polymorphism (20) mPing has not been inserted, the sample has a dream and is likely to be a variety other than Kinuhikari and Nasuhikari, or the sample has a dream and Kinuhikari And it can be determined that there is a high possibility of including varieties other than Nasuhikari.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (21) confirmed the presence or absence of mPing insertion in polymorphism (21). Can be identified. For example, when it is confirmed that mPing of the polymorphism (21) is inserted, the sample is likely to be at least one selected from the group consisting of masochistic, hanaetizen and hashimo, or the sample Is likely to contain one or more selected from the group consisting of: On the other hand, if it is confirmed that the polymorphic (21) mPing is not inserted, the sample is likely to be a varieties other than Busyoji, Hanaetisen and Hashishima, or the sample is foundered, Hanaetisen and It can be determined that there is a high possibility of including varieties other than Hashishima.

  The presence or absence of mPing insertion in the polymorphism (22) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (22). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (22) is inserted, it can be determined that the sample is likely to be breastfish or the sample is likely to contain breastfish. . On the other hand, if it is confirmed that the polymorphic (22) mPing has not been inserted, the sample is likely to be of a variety other than Furumiko, or the sample may include a variety other than Furumiko Judgment is high.

  The presence or absence of mPing insertion in polymorphism (23) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (23). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (23) is inserted, it can be determined that the sample is likely to be breastfish or the sample is likely to contain breastfish. . On the other hand, if it is confirmed that the polymorphic (23) mPing has not been inserted, the sample is likely to be of a variety other than Furumiko, or the sample may include a breed other than Furumiko Judgment is high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (24) confirmed the presence or absence of mPing insertion in polymorphism (24). Can be identified. For example, when it is confirmed that mPing of the polymorphism (24) is inserted, the sample is one or more selected from the group consisting of Hoshino Yume, Natsutsuboshi, Kirara 397, Yumepirika and Orozuki. It can be determined that there is a high possibility, or that the sample is likely to contain one or more selected from the group consisting of Hoshino Yume, Nanatsubo, Kirara 397, Yumepirika and Orozuki. On the other hand, if it is confirmed that the polymorphic (24) mPing has not been inserted, the sample is likely to be of a variety other than Hoshino Yume, Nanatsuboshi, Kirara 397, Yume Pirika and Orozuki. Alternatively, it can be determined that there is a high possibility that the sample includes varieties other than Hoshino Yume, Nanatsuboshi, Kirara 397, Yumepirika, and Oborozuki.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (25) confirmed the presence or absence of mPing insertion in polymorphism (25). Can be identified. For example, when it is confirmed that the polymorphic (25) mPing is inserted, the sample is at least one selected from the group consisting of a muscular, straight, sasanishiki, tatsukomochi and kinu. It can be determined that there is a high possibility, or the sample is likely to contain one or more selected from the group consisting of muddy, straight, sasanishiki, tatsukomochi, and kinu. On the other hand, if it is confirmed that the polymorphic (25) mPing has not been inserted, the sample is likely to be a variety other than the noodles, makigura, sasanishiki, tatsukomochi and kinu. Alternatively, it can be determined that there is a high possibility that the sample contains varieties other than noodles, makigura, sasanishiki, tatsukomochi and kinu-no-nade.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (26) confirmed the presence or absence of mPing insertion in polymorphism (26). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (26) is inserted, it can be determined that the sample is highly likely to be a slime or that the sample is likely to contain a slime. On the other hand, if it is confirmed that the mPing of the polymorphism (26) is not inserted, there is a high possibility that the sample is of a variety other than kinmusume, or the sample may include varieties other than kinmusume. Can be determined to be high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (27) confirmed the presence or absence of mPing insertion in polymorphism (27). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (27) is inserted, it can be determined that the sample is highly likely to be Ichino Kaori or that the sample is likely to contain Ichino Kaori. . On the other hand, if it is confirmed that the polymorphism (27) mPing is not inserted, the sample is likely to be of a variety other than Ichino Kaori, or the sample may contain a variety other than Ichino Kaori Judgment is high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (28) confirmed the presence or absence of mPing insertion in the polymorphism (28). Can be identified. For example, when it is confirmed that the polymorphic (28) mPing is inserted, it can be determined that the sample is highly likely to be rough or that the sample is likely to contain rough. On the other hand, if it is confirmed that the polymorphic (28) mPing is not inserted, it is highly possible that the sample is of a variety other than rough, or that the sample is likely to contain a variety other than rough. Can be judged.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (29) confirmed the presence or absence of mPing insertion in the polymorphism (29). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (29) is inserted, it can be determined that the sample is highly likely to be rough or the sample is highly likely to contain rough. On the other hand, when it is confirmed that the polymorphic (29) mPing has not been inserted, it can be determined that the sample is likely to be of a variety other than rough, or that the sample is likely to contain a variety other than rough. .

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (30) confirmed the presence or absence of mPing insertion in polymorphism (30). Can be identified. For example, when it is confirmed that the polymorphic (30) mPing is inserted, it can be determined that the sample is highly likely to be Yume Mizuho or that the sample is likely to contain Yume Mizuho. On the other hand, if it is confirmed that the polymorphic (20) mPing is not inserted, the sample is likely to be of a variety other than Yume Mizuho, or is likely to contain a variety other than Yume Mizuho. Can be judged.

  The presence or absence of mPing insertion in the polymorphism (31) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (31). Can be identified. For example, when it is confirmed that the polymorphic (31) mPing is inserted, it can be determined that the sample is highly likely to be Sasanishiki or that the sample is likely to contain Sasanishiki. On the other hand, if it is confirmed that the polymorphic (31) mPing is not inserted, the sample is likely to be a variety other than Sasanishiki, or the sample is likely to contain a variety other than Sasanishiki. Can be judged.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (32) confirmed the presence or absence of mPing insertion in polymorphism (32). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (32) is inserted, it can be determined that the sample is likely to be a milky queen, or the sample is likely to include a milky queen. On the other hand, if it is confirmed that the polymorphic (32) mPing is not inserted, the sample is likely to be of a breed other than the Milky Queen, or the sample may contain a breed other than the Milky Queen. Can be determined to be high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (33) confirmed the presence or absence of mPing insertion in the polymorphism (33). Can be identified. For example, when it is confirmed that the polymorphic (33) mPing is inserted, it can be determined that the sample is likely to be a chickweed or the sample is likely to contain a chickweed. On the other hand, if it is confirmed that the polymorphic (33) mPing has not been inserted, the sample is likely to be of a variety other than chickweed, or the sample is likely to contain varieties other than chickweed. Can be judged.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (34) confirmed the presence or absence of mPing insertion in the polymorphism (34). Can be identified. For example, when it is confirmed that the polymorphic (34) mPing is inserted, it can be determined that the sample is likely to be a chickweed or the sample is likely to contain a chickweed. On the other hand, if it is confirmed that the polymorphic (34) mPing has not been inserted, the sample is likely to be of a variety other than chickweed, or the sample is likely to contain varieties other than chickweed. Can be judged.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (35) confirmed the presence or absence of mPing insertion in the polymorphism (35). Can be identified. For example, when it is confirmed that mPing of the polymorphism (35) is inserted, there is a possibility that the sample is one or more selected from the group consisting of Himenomochi, Nikkomaru, Manamusume, Chiyonishiki and Wataboshi. It can be determined that the sample is high or that the sample is likely to contain one or more selected from the group consisting of Himenomochi, Nikkomaru, Manamusume, Chiyonishiki, and Wataboshi. On the other hand, if it is confirmed that the polymorphic (35) mPing has not been inserted, the sample is likely to be a variety other than Himenomochi, Nikkomaru, Manamusume, Chiyonishiki and Wataboshi, or the sample is It can be determined that there is a high possibility of including varieties other than Himenomochi, Nikkomaru, Manamusume, Chiyonoshiki and Wataboshi.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (36) confirmed the presence or absence of mPing insertion in the polymorphism (36). Can be identified. For example, when it is confirmed that the polymorphic (36) mPing has been inserted, it can be determined that the sample is likely to have a koji or the sample is likely to contain a koji. On the other hand, if it is confirmed that the polymorphic (36) mPing has not been inserted, the sample is likely to be of a variety other than kokomochi or the sample may contain a non-kaga mochi type. Can be determined to be high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (37) confirmed the presence or absence of mPing insertion in the polymorphism (37). Can be identified. For example, when it is confirmed that the polymorphic (37) mPing is inserted, it can be determined that the sample is likely to have a koji or the sample is likely to contain a koji. On the other hand, if it is confirmed that the polymorphic (37) mPing is not inserted, the sample is likely to be of a variety other than kokomochi, or the sample may contain a variety other than kagamochi. Can be determined to be high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (38) confirmed the presence or absence of mPing insertion in the polymorphism (38). Can be identified. For example, when it is confirmed that the polymorphic (38) mPing is inserted, it is highly likely that the sample is one or more selected from the group consisting of wind pups, snails and nikkomaru, or It can be determined that the sample is likely to contain one or more species selected from the group consisting of a child of the wind, a bruise and a nikkomaru. On the other hand, if it is confirmed that the polymorphic (38) mPing has not been inserted, the sample is likely to be a variety other than a wind child, or a sample other than a silkworm, or a sample, or the sample has a wind child. It can be determined that there is a high possibility of including varieties other than daughters and nikkomaru.

  The presence or absence of mPing insertion in the polymorphism (39) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (39). Can be identified. For example, when it is confirmed that the polymorphic (39) mPing is inserted, it can be determined that the sample is highly likely to be cotton bud, or the sample is likely to contain cotton bud. On the other hand, if it is confirmed that the polymorphic (39) mPing is not inserted, the sample is likely to be of a variety other than cotton bud, or the sample is likely to contain a variety other than cotton bud. Can be judged.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (40) confirmed the presence or absence of mPing insertion in polymorphism (40). Can be identified. For example, when it is confirmed that the polymorphic (40) mPing is inserted, it can be determined that the sample is highly likely to be cotton or the sample is likely to contain cotton. On the other hand, when it is confirmed that the polymorphic (40) mPing has not been inserted, it can be determined that the sample is likely to be of a variety other than cotton bud, or that the sample is likely to contain a variety other than cotton bud. .

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (41) confirmed the presence or absence of mPing insertion in polymorphism (41). Can be identified. For example, when it is confirmed that the polymorphic (41) mPing has been inserted, it can be determined that the sample is likely to contain or have a high possibility of containing it. . On the other hand, if it is confirmed that the polymorphic (41) mPing is not inserted, it is highly possible that the sample is of a variety other than hachi mochi or the sample is of a type other than hachi mochi. It can be determined that the possibility of inclusion is high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (42) confirmed the presence or absence of mPing insertion in the polymorphism (42). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (42) is inserted, the sample is highly likely to have a scab, or the sample is likely to include a scab Can be judged. On the other hand, if it is confirmed that the polymorphic (42) mPing is not inserted, it is highly possible that the sample is of a variety other than hachimochi or the sample is of a type other than hachimochi. It can be determined that the possibility of inclusion is high.

  The presence or absence of mPing insertion in polymorphism (43) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (43). Can be identified. For example, if it is confirmed that the mPing of polymorphism (43) is inserted, the sample is likely to be sticky and / or uncooked or the sample is sticky and / or Alternatively, it can be determined that there is a high possibility of including a bare bran. On the other hand, if it is confirmed that the polymorphic (43) mPing has not been inserted, the sample is likely to be of a variety other than Tatsukochi and Kinuhara, or the sample is It can be judged that there is a high possibility of including varieties other than Kinu Hata.

  The presence or absence of mPing insertion in the polymorphism (44) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (44). Can be identified. For example, when it is confirmed that the mPing of the polymorphism (44) is inserted, it can be determined that the sample is likely to have a feeling of coming or that the sample is likely to contain feeling of coming. . On the other hand, if it is confirmed that the polymorphic (44) mPing has not been inserted, it is highly possible that the sample is of a variety other than Kitayukimochi, or the sample may include a variety other than Kitayukimochi Judgment is high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (45) confirmed the presence or absence of mPing insertion in polymorphism (45). Can be identified. For example, when it is confirmed that the polymorphic (45) mPing is inserted, it is highly likely that the sample is a bare shell, or the sample is likely to contain a bare shell Can be judged. On the other hand, if it is confirmed that the polymorphic (45) mPing has not been inserted, it is highly possible that the sample is of a variety other than uncooked or that the sample is not uncaught. It can be determined that the possibility of inclusion is high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (46) confirmed the presence or absence of mPing insertion in polymorphism (46). Can be identified. For example, when it is confirmed that the polymorphic (46) mPing is inserted, it can be determined that the sample is likely to be Yamada Nishiki, or the sample is likely to include Yamada Nishiki. On the other hand, if it is confirmed that the polymorphic (46) mPing is not inserted, there is a high possibility that the sample is a variety other than Yamada Nishiki, or the sample may include a variety other than Yamada Nishiki. Can be determined to be high.

  The presence or absence of mPing insertion in the polymorphism (47) was confirmed by an amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (47). Can be identified. For example, when it is confirmed that the polymorphic (47) mPing is inserted, it can be determined that the sample is likely to be Yamada Nishiki, or the sample is likely to include Yamada Nishiki. On the other hand, if it is confirmed that the polymorphic (47) mPing is not inserted, there is a high possibility that the sample is a variety other than Yamada Nishiki, or the sample may include a variety other than Yamada Nishiki. Can be determined to be high.

  An amplification reaction using at least a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (48) confirmed the presence or absence of mPing insertion in the polymorphism (48). Can be identified. For example, if it is confirmed that the polymorphic (48) mPing is inserted, the sample is likely to have 5 million stones, or the sample is likely to contain 5 million stones. Can be judged. On the other hand, if it is confirmed that the polymorphic (48) mPing has not been inserted, it is highly possible that the sample is of a variety other than 500 million stones, or the sample is of a variety other than 500 million stones. It can be determined that the possibility of inclusion is high.

At the time of product type identification, it is preferable to perform any one of the following steps [1] to [42]. Process [1]-[42] should just perform any one process, and may carry out combining two or more processes according to the object. In addition, process [1]-[42] is only an example, and based on said Tables 2-5, the kind of rice can be identified also in combinations other than the polymorphic combination shown in these processes.
Step [1]: When the mPing is inserted in the polymorphism (1), it is determined that the test subject is at a glance or tender. In the step [1], the mPing in the polymorphism (1) is determined. It is preferable to use a primer set composed of two or more primers capable of detecting the presence or absence of insertion of mPing in the polymorphism (1) or a probe capable of detecting the presence or absence of insertion of DNA, and by amplification of DNA using the primer set It is preferable to perform an inspection. If the insertion of mPing in the polymorphism (1) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be spilled or tender, or the sample may contain squeeze or tender Judgment is high. If the insertion of mPing in the polymorphism (1) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than the first and the second, or the sample is other than the first and the second. It can be determined that there is a high possibility of including the varieties

Step [2]: mPing is inserted in polymorphism (2), and mPing is inserted in polymorphism (6), (12), (25) or (31), and (36) or (37). When not inserted, it is determined that the subject to be examined is Akitakomachi. In the step [2], the following may be performed.
A probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (2), or a probe set capable of detecting the presence or absence of mPing insertion in the polymorphism (2);
A probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (6), or a probe capable of detecting the presence or absence of mPing insertion in the polymorphism (6);
A probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (12) or the presence or absence of mPing insertion in the polymorphism (12);
A probe that can detect the presence or absence of mPing insertion in the polymorphism (25), or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (25), and / or polymorphism A probe that can detect the presence or absence of mPing insertion in (31), or a primer set comprising two or more primers that can detect the presence or absence of mPing insertion in polymorphism (31); and mPing in polymorphism (36) A probe that can detect the presence or absence of insertion of mPing, or a primer set composed of two or more primers that can detect the presence or absence of insertion of mPing in the polymorphism (36) and / or insertion of mPing in the polymorphism (37) A probe that can detect the presence or absence of mPing A primer set composed of two or more primers capable of detecting the presence or absence of insertion.

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. The amplification reaction of DNA using each primer set confirmed the insertion of mPing in polymorphism (2), and polymorphism (6), (12), (25) or (31), (36) or If the insertion of mPing is not confirmed in (37), it can be determined that there is a high possibility that the sample is a damaging town or that the sample is likely to contain a damaging town. The amplification reaction of DNA using each primer set does not confirm the insertion of mPing in polymorphism (2), or polymorphism (6), (12), (25) or (31), (36) or When the insertion of mPing is confirmed in any of (37), it can be determined that the sample is likely to be of a variety other than Akitamachi, or that the sample is likely to contain a variety other than Akitamachi.

Step [3]: mPing is inserted in polymorphism (3) and mPing is not inserted in polymorphism (6), (10) or (11) and (15) Is determined to be Hinohikari or Kuma no Mori, during step [3], the following may be used:
A primer set composed of a probe capable of detecting the presence or absence of mPing insertion in the polymorphism (3), or two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (2);
In the polymorphism (10), a probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (6), or the presence or absence of mPing insertion in the polymorphism (6) a probe that can detect the presence or absence of mPing insertion, or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (10), and the insertion of mPing in the polymorphism (11) One or more selected from a probe that can detect the presence or absence, or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (11); and mPing in the polymorphism (15) Probe that can detect the presence or absence of insertion of mPing in polymorphism (15) A primer set composed of two or more primers capable of detecting the presence or absence of.

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. When the amplification reaction of DNA using each primer set confirms the insertion of mPing in polymorphism (3), and the insertion of mPing is not confirmed in polymorphism (6), (10) or (11) and (15) Can be determined that the sample is likely to be Hinohikari or a forest bear, or that the sample is likely to include a Hinohikari or forest bear. Insertion of mPing in polymorphism (3) is not confirmed by amplification reaction of DNA using each primer set, or mPing in any of polymorphisms (6), (10) or (11) and (15) When the insertion is confirmed, it can be determined that the sample is likely to be a variety other than Hinohikari and Mori no Kuma, or the sample is likely to contain a variety other than Hinohikari and Mori no Kuma.

Step [4]: When mPing is inserted in the polymorphism (4) or (5), it is determined that the test subject is Nanatsuboshi. In the step [4], the polymorphism ( A probe that can detect the presence or absence of mPing insertion in 4) or a primer set comprising two or more primers that can detect the presence or absence of mPing insertion in polymorphism (4), or the mPing in polymorphism (5) It is preferable to use a probe set that can detect the presence or absence of insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (5), and a test by amplification of DNA using each primer set It is preferable to carry out. If the insertion of mPing in polymorphism (4) or (5) is confirmed by DNA amplification reaction using the primer set, the sample is highly likely to be a nerd, or the sample is a nerd It can be determined that there is a high possibility that the If the insertion of mPing in the polymorphisms (4) and (5) is not confirmed by the amplification reaction of DNA using the primer set, the sample is highly likely to be of a variety other than Nanatsubo or the sample is not It can be determined that there is a high possibility of including varieties other than Natsuboshi.

Step [5]: When the mPing is inserted in the polymorphisms (2) and (6), it is determined that the test subject is a punch. In the step [5], the polymorphism (2) A probe that can detect the presence or absence of mPing insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in polymorphism (2), and the presence or absence of mPing insertion in polymorphism (6) It is preferable to use a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (6), which can detect a polymorphism (6), and performing a test by amplification of DNA using each primer set It is preferable. When the insertion of mPing in polymorphisms (2) and (6) is confirmed by the amplification reaction of DNA using the primer set, the sample is highly likely to be an eclipse, or the sample may contain an eclipse Can be determined to be high. If the insertion of mPing in the polymorphism (2) or (6) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than Baeuki or the sample is of a variety other than Bae It is possible to determine that there is a high possibility of including.

Step [6]: When mPing is inserted in polymorphisms (7) and (8), it is determined that the test object is Kirara 397. In the case of step [6], polymorphism (7) A probe set that can detect the presence or absence of mPing insertion in the polymorphism (7) or a primer set comprising two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (7), and the insertion of the mPing in the polymorphism (8) It is preferable to use a probe set that can detect the presence or absence of a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in polymorphism (8). Preferably it is done. If insertion of mPing in polymorphisms (7) and (8) is confirmed by DNA amplification reaction using the primer set, the sample is likely to be Kirara 397, or the sample may contain Kirara 397 Judgment is high. If the insertion of mPing in polymorphism (7) or (8) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than Kirara 397, or the sample is other than Kirara 397. It can be determined that there is a high possibility of including the varieties.

Step [7]: A step of determining that the test object is straight when mPing is inserted in the polymorphism (9) and no mPing is inserted in the polymorphism (43). Include a probe that can detect the presence or absence of mPing insertion in the polymorphism (9), or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (9); Preferably, a probe that can detect the presence or absence of mPing insertion in the type (43) or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (43) is used. It is preferable to conduct a test by amplification of DNA using a primer set. If the insertion of mPing in the polymorphism (9) is confirmed by the amplification reaction of DNA using the primer set, and the insertion of mPing in the polymorphism (43) is not confirmed, the sample is highly likely to be true. Alternatively, it can be determined that the sample is likely to contain a straight line. If the insertion of mPing in the polymorphism (9) is not confirmed by the amplification reaction of DNA using each primer set, or if the insertion of mPing is confirmed in the polymorphism (43), the sample is a cultivar other than true It can be determined that there is a high possibility that the sample is likely to contain varieties other than the sample.

Step [8]: When the mPing is inserted in the polymorphism (10) or (11) and the mPing is not inserted in the polymorphism (3), it is determined that the test subject is a fragile step. In the case of [8], the following may be used.
A probe that can detect the presence or absence of mPing insertion in the polymorphism (10), or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (10), or a polymorphism (11 A probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (11);
A primer set comprising a probe capable of detecting the presence or absence of mPing insertion in the polymorphism (3) or two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (3).

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. If the insertion of mPing in polymorphism (10) or (11) is confirmed by the amplification reaction of DNA using each primer set, and the insertion of mPing is not confirmed in polymorphism (3), the sample should be It can be determined that there is a high possibility that the sample is likely to be present or that the sample is likely to contain a severe splash. If the DNA amplification reaction using each primer set does not confirm the insertion of mPing in polymorphisms (10) and (11), or if the insertion of mPing is confirmed in polymorphism (3), the sample is It is possible to determine that there is a high possibility that the product is a variety other than the above, or that there is a high possibility that the sample contains a product other than the fragile sample.

Step [9]: When mPing is inserted in the polymorphisms (2) and (12), it is determined that the test subject is a tangible romance. In the case of the step [9], the polymorphism (2) A primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (2), or a probe set capable of detecting the presence or absence of mPing insertion in polymorphism (2), and mPing insertion in polymorphism (12) It is preferable to use a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (12), or a probe that can detect the presence or absence, and a test by amplification of DNA using each primer set Preferably it is done. When insertion of mPing in polymorphisms (2) and (12) is confirmed by amplification reaction of DNA using the primer set, the sample is likely to be a romance, or the sample may contain a romance Judgment is high. If the insertion of mPing in the polymorphism (2) or (12) is not confirmed by the amplification reaction of DNA using the primer set, it is highly possible that the sample is a variety other than the romantic to which the sample is connected, or other than the romantic to which the sample is connected It can be determined that there is a high possibility of including the varieties.

Step [10]: When mPing is inserted in polymorphism (13), it is determined that the test subject is Kinuhikari. In step [10], insertion of mPing in polymorphism (13) It is preferable to use a probe set that can detect the presence or absence of a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (13), and a test by amplification of DNA using the primer set is performed. It is preferable. When insertion of mPing in the polymorphism (13) is confirmed by the amplification reaction of DNA using the primer set, it can be determined that the sample is highly likely to be Kinuhikari or that the sample is likely to contain Kinuhikari. . If the insertion of mPing in the polymorphism (13) is not confirmed by DNA amplification using the primer set, the sample is likely to be of a variety other than Kinuhikari, or the sample may contain a variety other than Kinuhikari Can be determined to be high.

Step [11]: When mPing is inserted in polymorphism (14), it is determined that the test subject is a dream of Asahi. In step [11], mPing of polymorphism (14) is determined. It is preferable to use a probe set that can detect the presence or absence of insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (14), and a test by amplification of DNA using the primer set It is preferable to carry out. If the insertion of mPing in the polymorphism (14) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a dream of Asahi, or the sample may contain a dream of Asahi Can be determined to be high. If the insertion of mPing in the polymorphism (14) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a variety other than the dream of Asahi, or the sample is a variety other than the dream of Asahi It can be determined that there is a high possibility of

Step [12]: When mPing is inserted in polymorphisms (8) and (15), it is determined that the test subject is dreamy. In the case of step [12], polymorphism (8) A probe set that can detect the presence or absence of mPing insertion in the polymorphism (8) or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (8); It is preferable to use a probe that can detect the presence or absence of a primer set composed of two or more primers that can detect the presence or absence of insertion of mPing in the polymorphism (15), and performs an inspection by DNA amplification using each primer set. It is preferable. When insertion of mPing in polymorphisms (8) and (15) is confirmed by DNA amplification reaction using the primer set, the sample is likely to be dreamy or the sample may contain dreamy Judgment is high. If insertion of mPing in polymorphism (8) or (15) is not confirmed by DNA amplification reaction using the primer set, the sample is likely to be of a variety other than dreamy or the sample is other than dreamy. It can be determined that there is a high possibility of including the varieties.

Step [13]: When mPing is inserted in the polymorphism (16), it is determined that the test object is fungus. In the step [13], mPing in the polymorphism (16) is determined. It is preferable to use a probe set that can detect the presence or absence of insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (16), and a test by amplification of DNA using the primer set It is preferable to carry out. If the insertion of mPing in the polymorphism (16) is confirmed by the amplification reaction of DNA using the primer set, the sample is highly likely to be fungus or the sample may contain fungus. Can be determined to be high. If the insertion of mPing in the polymorphism (16) is not confirmed by the amplification reaction of DNA using the primer set, it is highly possible that the sample is of a variety other than Fusakogane, or the sample is of a type other than Fusakogane. It is possible to determine that there is a high possibility of including.

Step [14]: When mPing is inserted in the polymorphism (17) or (18), it is determined that the test subject is a princess princess. In the step [14], the polymorphism (17) A primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (17) or a primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (17) It is preferable to use a probe that can detect the presence or absence of a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (18), and a test is performed by DNA amplification using each primer set. It is preferable. If insertion of mPing in polymorphism (17) or (18) is confirmed by DNA amplification reaction using the primer set, the sample is likely to be a princess or the sample may contain a princess Judgment is high. If the insertion of mPing in polymorphisms (17) and (18) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than Tsutsuhime, or the sample is other than Tsuyahime It can be determined that there is a high possibility of including the varieties.

Step [15]: When mPing is inserted in the polymorphism (19), it is determined that the test subject is Hana etizen. In the step [15], the insertion of mPing in the polymorphism (19) is determined. It is preferable to use a probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (19), or a probe capable of detecting the presence or absence, and performing a test by DNA amplification using the primer set It is preferable. When the insertion of mPing in the polymorphism (19) is confirmed by the amplification reaction of DNA using the primer set, it can be determined that the sample is likely to be hanaethiene or the sample is likely to contain hanaethizen . If the insertion of mPing in the polymorphism (19) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than Hanaezen, or the sample may contain a variety other than Hanaezen Can be determined to be high.

Step [16]: When mPing is inserted in the polymorphism (20) and mPing is not inserted in the polymorphism (12), it is determined that the test subject is a dream horsetail Step [16] In this case, a probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (20), or a probe set capable of detecting the presence or absence of mPing insertion in the polymorphism (20), and It is preferable to use a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (12), or a probe that can detect the presence or absence of mPing insertion in the polymorphism (12), It is preferable to perform a test by amplification of DNA using each primer set. If the insertion of mPing in the polymorphism (20) is confirmed by the amplification reaction of DNA using the primer set, and the insertion of mPing in the polymorphism (12) is not confirmed, the sample may be a dream. It can be determined that the sample is high or the sample is likely to contain a dream horsetail. If the insertion of mPing in the polymorphism (20) is not confirmed by the amplification reaction of DNA using each primer set, or if the insertion of mPing is confirmed in the polymorphism (12), the sample should be of a variety other than Yumeshishishi It can be determined that there is a high possibility that there is a high possibility that the sample includes a variety other than a dream dream.

Step [17]: When the mPing is inserted in the polymorphism (21) and the mPing is not inserted in the polymorphism (19), it is determined that the test subject is a bath maiden or a hashimo step [17] ] A primer set comprising two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (21), or the presence or absence of mPing insertion in the polymorphism (21), It is preferable to use a probe set that can detect the presence or absence of mPing insertion in the polymorphism (19) or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (19). It is preferable to conduct a test by amplification of DNA using each primer set. If the insertion of mPing in the polymorphism (21) is confirmed by the amplification reaction of DNA using the primer set, and the insertion of mPing in the polymorphism (19) is not confirmed, the sample may be a peasant or a hashimo It is possible to determine that the sample is highly likely or that the sample is likely to contain a bath maiden or a mustard. If the insertion of mPing in the polymorphism (21) is not confirmed by the amplification reaction of DNA using each primer set, or if the insertion of mPing is confirmed in the polymorphism (19), the sample is other than Fusumoto and Hashishima. It can be determined that there is a high possibility of being a variety, or that a sample is likely to contain varieties other than Fusotome and Hashishima.

Step [18]: When mPing is inserted in the polymorphism (22) or (23), it is determined that the subject to be tested is a fluke. In the step [18], the polymorphism (22 ) A probe that can detect the presence or absence of mPing insertion in polymorphism (22), or a primer set comprising two or more primers that can detect the presence or absence of mPing insertion in polymorphism (22), or the insertion of mPing in polymorphism (23) It is preferable to use a probe that can detect the presence or absence of a primer or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (23). Preferably it is done. If the insertion of mPing in the polymorphism (22) or (23) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be full or the sample is It can be determined that the possibility of inclusion is high. If the insertion of mPing in the polymorphisms (22) and (23) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than Furumoko, or the sample is swollen It can be determined that there is a high possibility of including other varieties.

Step [19]: When the mPing is inserted in the polymorphism (7) , (15), and (24), it is determined that the subject to be tested is Hoshino Yume. Can be done using:
A primer set comprising a probe capable of detecting the presence or absence of mPing insertion in the polymorphism (7) or two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (7) ;
A primer set composed of a probe capable of detecting the presence or absence of mPing insertion in the polymorphism (15) or two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (15); and
A primer set composed of a probe that can detect the presence or absence of mPing insertion in the polymorphism (24) or two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (24).

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. If the insertion of mPing in polymorphisms (7) or (15) and (24) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a dream or the sample is It can be determined that there is a high possibility of including Hoshino Yume. If the insertion of mPing in any of polymorphisms (7), (15), and (24) is not confirmed by the amplification reaction of DNA using the primer set, the sample may be of a variety other than Hoshino Yume Or the sample is likely to contain varieties other than Hoshino Yume.

Step [20]: When mPing is inserted in the polymorphism (25) and mPing is not inserted in the polymorphisms (2) and (9), it is determined that the test subject is unsightly. For [20], the following may be used:
A primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (25) or the presence or absence of mPing insertion in the polymorphism (25);
A probe that can detect the presence or absence of mPing insertion in the polymorphism (2), or a primer set comprising two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (2); and the polymorphism (9) A probe set comprising two or more primers that can detect the presence or absence of mPing insertion in polymorphism (9) or a probe that can detect the presence or absence of mPing insertion in polymorphism (9).

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. If the amplification reaction of DNA using each primer set confirms the insertion of mPing in polymorphism (25) and the insertion of mPing is not confirmed in polymorphisms (2) and (9), the sample may be cumbersome It is possible to determine that there is a high possibility that the sample has a high probability or a sample is difficult to contain. If the amplification reaction of DNA using each primer set does not confirm the insertion of mPing in the polymorphism (15), or if the insertion of mPing is confirmed in the polymorphism (2) or (9), the sample is cumbersome. It is possible to determine that there is a high possibility that it is a variety other than the above, or that there is a high possibility that the sample contains a variety other than that which is not healthy.

Step [21]: When mPing is inserted in the polymorphism (26), it is determined that the subject to be tested is a slime. In step [21], insertion of mPing in the polymorphism (26) It is preferable to use a probe set that can detect the presence or absence of a primer or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (26). Preferably it is done. When the insertion of mPing in the polymorphism (26) is confirmed by the amplification reaction of DNA using the primer set, the sample is highly likely to be dull or the sample is highly likely to contain dull Can be judged. If the insertion of mPing in the polymorphism (26) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than the slime, or the sample includes a variety other than the slime It can be determined that the possibility is high.

Step [22]: When the mPing is inserted in the polymorphism (27), it is determined that the test object is Aichi Kaori. In the step [22], the mPing of the polymorphism (27) is determined. It is preferable to use a probe set that can detect the presence or absence of insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (27), and a test by amplification of DNA using the primer set It is preferable to carry out. When the insertion of mPing in the polymorphism (27) is confirmed by the amplification reaction of DNA using the primer set, the sample is highly likely to be the one or the possibility that the sample contains the one. Can be determined to be high. If the insertion of mPing in the polymorphism (27) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a variety other than Kaori Kaori, or the sample is a variety other than Kaori Kaori. It is possible to determine that there is a high possibility of including.

Step [23]: When mPing is inserted in the polymorphism (28) or (29), it is determined that the test subject is rough. In the step [23], the polymorphism (28) A probe that can detect the presence or absence of mPing insertion or a primer set comprising two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (28), or the presence or absence of mPing insertion in the polymorphism (29) It is preferable to use a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (29) or a probe capable of detecting DNA, and performing a test by DNA amplification using each primer set Is preferred. If the insertion of mPing in polymorphism (28) or (29) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be rough or the sample may contain rough Can be determined to be high. If the insertion of mPing in the polymorphisms (28) and (29) is not confirmed by the amplification reaction of DNA using the primer set, it is highly possible that the sample is of a variety other than rough or the sample is of a variety other than rough It is possible to determine that there is a high possibility of including.

Step [24]: When mPing is inserted in the polymorphism (30), it is determined that the test object is Yume Mizuho. In step [24], insertion of mPing in the polymorphism (30) It is preferable to use a probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (30) or a probe capable of detecting the presence or absence of the polymorphism (30). Preferably it is done. When insertion of mPing in the polymorphism (30) is confirmed by the amplification reaction of DNA using the primer set, the sample is highly likely to be Yume Mizuho, or the sample is likely to contain Yume Mizuho Can be judged. If the insertion of mPing in the polymorphism (30) is not confirmed by the DNA amplification reaction using the primer set, the sample is likely to be of a variety other than Yume Mizuho, or the sample contains a variety other than Yume Mizuho It can be determined that the possibility is high.

Step [25]: When mPing is inserted in polymorphism (31), it is determined that the test subject is Sasanishiki. In step [25], the insertion of mPing in polymorphism (31) is determined. It is preferable to use a probe set that can detect the presence or absence of a primer set composed of two or more primers that can detect the presence or absence of insertion of mPing in the polymorphism (31), and a test is performed by amplification of DNA using the primer set. It is preferable. When the insertion of mPing in the polymorphism (31) is confirmed by the amplification reaction of DNA using the primer set, it can be determined that the sample is highly likely to be Sasanishiki or that the sample is likely to contain Sasanishiki . If the insertion of mPing in the polymorphism (31) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a variety other than Sasanishiki, or the sample may contain a variety other than Sasanishiki Can be determined to be high.

Step [26]: When mPing is inserted in the polymorphism (32), it is determined that the test object is a milky queen. In step [26], insertion of mPing in the polymorphism (32) It is preferable to use a probe set that can detect the presence or absence of a primer or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (32). Preferably it is done. When the insertion of mPing in the polymorphism (32) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a milky queen, or the sample is likely to contain a milky queen. Can be judged. If insertion of mPing in the polymorphism (32) is not confirmed by DNA amplification reaction using the primer set, the sample is likely to be of a variety other than Milky Queen, or the sample contains a variety other than Milky Queen It can be determined that the possibility is high.

Step [27]: When mPing is inserted in polymorphism (33) or (34), it is determined that the test subject is a chickworm. In step [27], in polymorphism (33) A probe that can detect the presence or absence of mPing insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (33), or the presence or absence of mPing insertion in the polymorphism (34) It is preferable to use a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the probe or polymorphism (34) that can detect the polymorphism (34), and performing a test by DNA amplification using each primer set Is preferred. If the insertion of mPing in polymorphism (33) or (34) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a chickworm or the sample may contain a chickweed. Can be determined to be high. If the insertion of mPing in the polymorphisms (33) and (34) is not confirmed by the amplification reaction of DNA using the primer set, it is highly possible that the sample is a variety other than chickweed or the sample is a cultivar other than chickweed. It is possible to determine that there is a high possibility of including.

Step [28]: When the mPing is inserted in the polymorphism (35) and the mPing is not inserted in the polymorphism (12) or (38) and (39) or (40), the test subject is Determining that it is Himenomochi, Manamusume or Chiyonoshiki In the step [28], the following may be performed.
A probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (35) or the presence or absence of mPing insertion in the polymorphism (35);
A probe that can detect the presence or absence of mPing insertion in the polymorphism (12), or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (12), and / or polymorphism A probe that can detect the presence or absence of insertion of mPing in (38), or a primer set comprising two or more primers that can detect the presence or absence of insertion of mPing in polymorphism (38); and mPing in polymorphism (39) A probe that can detect the presence or absence of insertion, or a primer set comprising two or more primers that can detect the presence or absence of insertion of mPing in the polymorphism (39) and / or insertion of mPing in the polymorphism (40) A probe capable of detecting the presence or absence of mPing in the polymorphism (40) A primer set composed of two or more primers capable of detecting the presence or absence of insertion.

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. The amplification reaction of DNA using each primer set confirmed the insertion of mPing in polymorphism (35), and the insertion of mPing was not confirmed in polymorphisms (12) or (38) and (39) or (40) In some cases, it can be determined that the sample is likely to be Himenomochi, Manamusume or Chiyonishiki, or that the sample is likely to contain Himenomochi, Manamusume or Chiyonishiki. Insertion of mPing in polymorphism (35) is not confirmed by amplification reaction of DNA using each primer set, or insertion of mPing in either polymorphism (12) or (38) and (39) or (40) Is confirmed, it can be determined that the sample is likely to be a variety other than Himenomochi, Manamusume and Chiyonishiki, or that the sample is likely to contain a variety other than Himenomochi, Manamusume and Chiyonishiki.

Step [29]: When mPing is inserted in the polymorphism (36) or (37), it is determined that the subject to be tested is kokomochi. In the step [29], the polymorphism (36) A primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (36), or a primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (37) It is preferable to use a probe that can detect the presence or absence of a primer set composed of two or more primers that can detect the presence or absence of insertion of mPing in the polymorphism (37), and perform an inspection by DNA amplification using each primer set. It is preferable. If the insertion of mPing in the polymorphism (36) or (37) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to have a koji or the sample may contain a koji Judgment is high. If the insertion of mPing in polymorphisms (36) and (37) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than kojimochi, or the sample is not kojimochi It can be determined that there is a high possibility of including the varieties.

Step [30]; When mPing is inserted in polymorphism (3) or (15) and (38), it is determined that the test subject has a child of wind. You can do this with:
A primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (3), or a primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (3), or polymorphism (15) A probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (15);
A primer set composed of a probe that can detect the presence or absence of mPing insertion in the polymorphism (38) or two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (38).

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. When the insertion of mPing in polymorphisms (3) or (15) and (38) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to have a wind child, or the sample is It can be determined that there is a high possibility of including a child of wind. If the insertion of mPing in any of polymorphisms (3), (15), and (38) is not confirmed by the amplification reaction of DNA using the primer set, the sample may be of a variety other than Koshi no Mochimochi Or the sample is likely to contain varieties other than the wind puppy.

Step [31]: When mPing is inserted in the polymorphism (39) or (40), it is determined that the test subject is cotton bud. In the step [31], in the polymorphism (39) A probe that can detect the presence or absence of mPing insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (39), or the presence or absence of mPing insertion in the polymorphism (40) It is preferable to use a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the probe or polymorphism (40) that can detect the polymorphism (40), and performing a test by DNA amplification using each primer set Is preferred. If the insertion of mPing in the polymorphism (39) or (40) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be cotton bud, or the sample may contain cotton bud. Can be determined to be high. If the insertion of mPing in the polymorphisms (39) and (40) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a variety other than cotton bud, or the sample is a variety other than cotton bud. It is possible to determine that there is a high possibility of including.

Step [32]: When mPing is inserted in the polymorphism (41) or (42), it is determined that the subject to be tested is squirting. In the step [32], the polymorphism ( 41) a probe that can detect the presence or absence of mPing insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (41), or the mPing in the polymorphism (42) It is preferable to use a probe set that can detect the presence or absence of insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (42), and a test by amplification of DNA using each primer set It is preferable to carry out. If the insertion of mPing in the polymorphism (41) or (42) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be swallowed or the sample is swollen It can be determined that there is a high possibility of including mochi. If the insertion of mPing in the polymorphisms (41) and (42) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be a variety other than the rice cake or the sample is It can be determined that there is a high possibility of including varieties other than rice cakes.

Step [33]: When the mPing is inserted in the polymorphism (43) and the mPing is not inserted in the polymorphism (45), it is determined that the test subject is tucky. Step [33] A primer set comprising two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (43), or a probe that can detect the presence or absence of mPing insertion in the polymorphism (43), and It is preferable to use a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (45), or a probe that can detect the presence or absence of mPing insertion in the polymorphism (45), It is preferable to perform a test by amplification of DNA using each primer set. If the insertion of mPing in the polymorphism (43) is confirmed by the amplification reaction of DNA using the primer set, and the insertion of mPing in the polymorphism (45) is not confirmed, the sample may be sticky Can be determined to be high, or the sample is likely to contain trumpet. If the insertion of mPing in the polymorphism (43) is not confirmed by the amplification reaction of DNA using each primer set, or if the insertion of mPing is confirmed in the polymorphism (45), the sample is of a variety other than Takomochi. It is possible to determine that the sample is likely to contain a variety other than Tatsukomochi.

Step [34]: When the mPing is inserted in the polymorphism (44), it is determined that the test subject is coming and going. In the step [34], the mPing of the polymorphism (44) is determined. It is preferable to use a probe set that can detect the presence or absence of insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (44), and a test by amplification of DNA using the primer set It is preferable to carry out. When the insertion of mPing in the polymorphism (44) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to have a feeling of coming, or the sample may have a feeling of coming. Can be determined to be high. If the insertion of mPing in the polymorphism (44) is not confirmed by the amplification reaction of DNA using the primer set, the sample is highly likely to be of a variety other than Yukimochi or the sample is of a variety other than Yukimochi It is possible to determine that there is a high possibility of including.

Step [35]: When mPing is inserted in the polymorphism (45), it is determined that the test subject is a bare skin. In the step [35], mPing in the polymorphism (45) is determined. It is preferable to use a probe that can detect the presence or absence of insertion of a primer or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (45), and by amplification of DNA using the primer set It is preferable to perform an inspection. If the insertion of mPing in the polymorphism (45) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be bare or the sample may contain bare Judgment is high. If the insertion of mPing in the polymorphism (45) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than uncooked or the sample is not uncooked It can be determined that there is a high possibility of including the varieties.

Step [36]: When mPing is inserted in the polymorphism (46) or (47), it is determined that the test object is Nishiki Yamada. In the step [36], the polymorphism (46) A primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (46) or a primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in polymorphism (46) or mPing insertion in polymorphism (47) It is preferable to use a probe that can detect the presence or absence of a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (47), and a test is performed by DNA amplification using each primer set. It is preferable. When insertion of mPing in polymorphism (46) or (47) is confirmed by amplification reaction of DNA using the primer set, the sample is likely to be Yamada Nishiki, or the sample may contain Yamada Nishiki Judgment is high. If the insertion of mPing in polymorphisms (46) and (47) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than Yamada Nishiki, or the sample is other than Yamada Nishiki It can be determined that there is a high possibility of including the varieties.

Step [37]: When mPing is inserted in polymorphism (48), it is determined that the test object is 500 million stones. In step [37], mPing in polymorphism (48) is determined. It is preferable to use a probe that can detect the presence or absence of insertion of a primer or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (48), by amplification of DNA using the primer set It is preferable to perform an inspection. If the insertion of mPing in the polymorphism (48) is confirmed by the amplification reaction of DNA using the primer set, the sample is likely to contain 500 million stones, or the sample may contain 500 million stones Judgment is high. If the insertion of mPing in the polymorphism (48) is not confirmed by DNA amplification reaction using the primer set, the sample is likely to be of a variety other than 500 million stones, or the sample is other than 500 million stones It can be determined that there is a high possibility of including the varieties.

Step [38]: When the mPing is inserted in the polymorphisms (35) and (38), it is determined that the test subject is lumpy. In the step [38], the polymorphism (35) A probe set capable of detecting the presence or absence of insertion of mPing in or a primer set comprising two or more primers capable of detecting the presence or absence of insertion of mPing in the polymorphism (35), and the insertion of mPing in the polymorphism (38) It is preferable to use a probe that can detect the presence or absence of a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (38), and perform an inspection by DNA amplification using each primer set. It is preferable. If the insertion of mPing in polymorphisms (35) and (38) is confirmed by DNA amplification reaction using the primer set, the sample is likely to contain or may contain sample Judgment is high. If the insertion of mPing in the polymorphism (35) or (38) is not confirmed by the amplification reaction of DNA using the primer set, it is highly possible that the sample is of a variety other than Nikko, or the sample is not It can be determined that there is a high possibility of including the varieties.

Step [39]: When mPing is inserted in the polymorphism (3) and (6), (10) or (11), it is determined that the test subject is a Yukinko dance step [39] 39] can be done using the following:
A primer set composed of a probe capable of detecting the presence or absence of mPing insertion in the polymorphism (3) or two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (3); and
A probe set that can detect the presence or absence of mPing insertion in the polymorphism (6) or a primer set comprising two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (6), mPing in the polymorphism (10) A probe set comprising two or more primers capable of detecting the presence or absence of insertion of mPing in the polymorphism (10) or the presence or absence of insertion of mPing in the polymorphism (11) One or more selected from the group consisting of a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the detectable probe or polymorphism (11).

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. If the insertion of mPing in polymorphism (3) and (6), (10) or (11) is confirmed by DNA amplification reaction using the primer set, the sample may be kinko dance It is possible to determine that the probability is high or the sample is likely to contain a kinko dance. If the insertion of mPing in polymorphism (3) and any of (6), (10) or (11) is not confirmed by DNA amplification reaction using the primer set, the sample is other than Yukinko Mai It is possible to determine that there is a high possibility that the sample is a variety of varieties, or that the sample is likely to include a variety other than Yukinko Mai.

Step [40]: When mPing is inserted in the polymorphisms (12) and (20), it is determined that the test object is a light source. In the step [40], the polymorphism (12) A probe that can detect the presence or absence of mPing insertion or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (12), and the presence or absence of mPing insertion in the polymorphism (20) It is preferable to use a primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (20) or a probe capable of detecting DNA, and performing a test by DNA amplification using each primer set Is preferred. When insertion of mPing in polymorphisms (12) and (20) is confirmed by DNA amplification reaction using the primer set, the sample is likely to be a light or the sample may contain a light Judgment is high. If the insertion of mPing in the polymorphism (12) or (20) is not confirmed by the amplification reaction of DNA using the primer set, the sample is likely to be of a variety other than Hikari, or the sample is other than Hikari. It can be determined that there is a high possibility of including the varieties.

Step [41]: mPing is inserted into the polymorphism (12), and the mPing is inserted into the polymorphism (2), (13), (20), (26), (35) or (38). In the case of the step [41], the following may be performed using the following.
A primer set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (12), or a probe capable of detecting the presence or absence of mPing insertion in the polymorphism (12),
A probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (2), or a probe capable of detecting the presence or absence of mPing insertion in the polymorphism (2);
A probe set comprising two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (13) or the probe capable of detecting the presence or absence of mPing insertion in the polymorphism (13);
A primer set composed of two or more primers capable of detecting the presence or absence of mPing insertion in the polymorphism (20) or the presence or absence of mPing insertion in the polymorphism (20);
A probe that can detect the presence or absence of mPing insertion in the polymorphism (26), or a primer set composed of two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (26); and the polymorphism (35) A probe that can detect the presence or absence of mPing insertion in the DNA, or a primer set comprising two or more primers that can detect the presence or absence of mPing insertion in the polymorphism (35), and / or mPing in the polymorphism (38) A probe set comprising two or more primers capable of detecting the presence or absence of insertion of mPing in the polymorphism (38).

  Among these, it is preferable to perform an inspection by amplification of DNA using each primer set. The amplification reaction of DNA using each primer set confirmed the insertion of mPing in polymorphism (12), and polymorphisms (2), (13), (20), (26), (35) Alternatively, when the insertion of mPing is not confirmed in (38), it can be determined that the sample is highly likely to be clear or that the sample is likely to contain clear light. Whether the insertion of mPing in polymorphism (12) is not confirmed by the amplification reaction of DNA using each primer set, or polymorphism (2), (13), (20), (26), (35) Or, when the insertion of mPing is confirmed in either of (38), it is determined that the sample is likely to be of a variety other than Akikari or that the sample is likely to contain a variety other than Akikari. it can.

Step [42]: When mPing is not inserted in any of the polymorphisms (1) to (48), the test object is Koshihikari, Akebono, Yume Obako, Nipponbare, Aya no Kagayaki, Yumezuku Judgment of autumn poems, cheering or morning sun.
In the step [42], all probes that can detect the presence or absence of mPing insertion in each of polymorphisms (1) to (48) or the presence or absence of mPing insertion in polymorphisms (1) to (48) It is preferable to use all primer sets composed of two or more primers that can be detected, and it is preferable to perform a test by amplification of DNA using the primer set. If the insertion of mPing in the polymorphisms (1) to (48) is not confirmed by the amplification reaction of DNA using the primer set, the sample is Koshihikari, Akebono, Yume Abiko, Nipponbare, Aya no Kagayaki, Yume Shizuku, It can be determined that it is more likely to be an autumn poem, a cheer, or a morning sun, or a sample is more likely to contain these varieties than Jabi. When the insertion of mPing in any of polymorphisms (1) to (48) is confirmed by DNA amplification reaction using the primer set, the sample is Koshihikari, Akebono, Yume Abiko, Nipponbare, Aya no Kagayaki, It is more likely to be of a variety other than Yume Shizuku, Sabi, autumn poetry, cheerfulness, and Asahi, or the sample is Koshihikari, Akebono, Yume Obako, Nipponbare, Aya no Kagayaki, Yume Shizuku, Sabi It is possible to determine that there is a high possibility of including varieties other than the poems, spirits and Asahi.

  The present invention also relates to the above-described various primer sets used in the present invention and a kit containing such various primer sets.

  The kit of the present invention may further contain a protein used in the amplification reaction. Examples of such proteins include DNA polymerase (eg, strand displacement DNA polymerase in LAMP), restriction enzyme, ribonuclease H, ligase, helicase, and recombinase. The kit of the present invention may also contain a fluorescent reagent, a substrate such as dNTPs, and a coenzyme such as ATP. The kit of the present invention further comprises a control DNA encoding a housekeeping gene (eg, GAPDH, β-actin, β2-microglobulin, HPRT1) that can be used as a control, and two or more primers for such control DNA. May be included. The kit of the present invention may also contain a positive control and / or a negative control. The kit of the present invention may contain each component in a form isolated in individual containers (eg, tubes), but two or more kinds of components may be mixed in advance in the same container.

  EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited to these Examples.

Example 1: Identification of varieties of Hitomebore by PCR method Koshihikari, Hitomebore, Akitakomachi, Hinohikari, Nanatsubo, Haenuki, Kirara 397 brown rice, which are the top seven varieties of Uchi rice, are ground in a food mill. DNA was extracted from 5 g using a GM Quicker 2 extraction kit.

  An equal amount of Koshihikari extracted DNA was mixed with the extracted DNA of the pupil, and a pseudo mixed sample into the pupil was prepared.

  Variety identification was performed using the PCR method. The primers used were as shown in Table 8.

  PCR reaction was 1 × Gotaq Green Master mix (Promega), 0.25 μM PCR-F and PCR-R primer, 10 μL mixed with 5 ng extracted DNA, 95 ° C. for 2 minutes, 30 cycles of 95 ° C. for 30 seconds; 55 ° C. 30 seconds; performed at 72 ° C. for 30 seconds and 72 ° C. for 5 minutes. The completed reaction solution was electrophoresed on a 2% agarose gel to confirm the amplified product.

  The results of the PCR method are shown in FIG. As is clear from FIG. 51, a 244 bp fragment indicating that there is no insertion is obtained in varieties other than the pupil, but an amplified fragment of 677 bp that is longer by the transposon sequence length (433 bp) can be obtained by the pupil, so that only the pupil can be easily removed. Were identifiable. In addition, since the amplified sample of both the pupils and Koshihikari was obtained, it was easy to determine that the pupils were mixed with other varieties.

Example 2: Identification of varieties of pupils by LAMP method After DNA extraction and preparation of a pseudo mixed sample were performed in the same manner as in Example 1, the following operation was performed.

  Variety identification was performed using the LAMP method. The primers used were as shown in Table 9.

In the LAMP reaction, a P primer set (35P-F3, 35P-B3, 35P-FIP, 35P-BIP, 35P-LF and 35P-LB) for detecting the presence of transposon mPing insertion in the polymorphism (1) and no insertion Two sets of N primer sets to be detected (35N-F3, 35N-B3, 35N-FIP, 35N-BIP, 35N-LF and 35N-LB) are used, and 20 mM Tris-HCl (pH 8.8), 10 mM KCl, respectively. 8 mM MgSO ₄ , 0.1% Tween 20, 0.8 M Betain, 1.4 mM dNTPs, 0.2 μM F3 and B3 primers, 1.6 μM FIP and BIP primers, 0.8 μM LF and LB primers, 8 units Bst DNA polymerase (New En land Biolabs), 1 [mu] L of Loopamp fluorescence-visual detection reagent (Eiken Chemical), and to make a total volume of 25μL containing the extracted DNA of 10 ng.
In the LAMP amplification, the reaction was stopped at 63 ° C. for 30 minutes and then heated at 80 ° C. for 2 minutes to stop the reaction. The reaction solution was irradiated with ultraviolet rays of 365 nm, and those that developed fluorescence were judged as having amplification, and those that did not develop were judged as having no amplification.

  The results of the LAMP method are shown in FIG. As is clear from FIG. 52, amplification was confirmed only for the P primer set and the N primer set for other varieties, so that it was easy to identify the pupil as with the PCR method. The same results as in Example 1 were obtained for the mixed sample.

  According to the present invention, by identifying rice varieties, it can be used to identify the varieties contained in the sample, detect the presence or absence of other varieties in the sample, determine the authenticity of the varieties displayed as products, etc. it can.

SEQ ID NO: 1: Nucleotide sequence in which mPing of polymorphism (1) is inserted (marker 35)
SEQ ID NO: 2: Base sequence in which mPing of polymorphism (2) is inserted (marker 39)
SEQ ID NO: 3: Base sequence in which mPing of polymorphism (3) is inserted (marker 26)
SEQ ID NO: 4: Base sequence in which mPing of polymorphism (4) is inserted (marker 52)
SEQ ID NO: 5: base sequence in which mPing of polymorphism (5) is inserted (marker 186)
SEQ ID NO: 6: base sequence in which mPing of polymorphism (6) is inserted (marker 233)
SEQ ID NO: 7: base sequence in which mPing of polymorphism (7) is inserted (marker 4)
SEQ ID NO: 8: base sequence in which mPing of polymorphism (8) is inserted (marker 189)
SEQ ID NO: 9: base sequence in which mPing of polymorphism (9) is inserted (marker 239)
SEQ ID NO: 10: base sequence in which mPing of polymorphism (10) is inserted (marker 240)
SEQ ID NO: 11: base sequence in which mPing of polymorphism (11) is inserted (marker 241)
SEQ ID NO: 12: base sequence in which mPing of polymorphism (12) is inserted (marker 68)
SEQ ID NO: 13: base sequence in which mPing of polymorphism (13) is inserted (marker 245)
SEQ ID NO: 14: base sequence in which mPing of polymorphism (14) is inserted (marker 253)
SEQ ID NO: 15: base sequence in which mPing of polymorphism (15) is inserted (marker 191)
SEQ ID NO: 16: base sequence in which mPing of polymorphism (16) is inserted (marker 261)
SEQ ID NO: 17: base sequence in which mPing of polymorphism (17) is inserted (marker 77)
SEQ ID NO: 18: base sequence in which mPing of polymorphism (18) is inserted (marker 265)
SEQ ID NO: 19: base sequence in which mPing of polymorphism (19) is inserted (marker 83)
SEQ ID NO: 20: base sequence in which mPing of polymorphism (20) is inserted (marker 244)
SEQ ID NO: 21: base sequence in which mPing of polymorphism (21) is inserted (marker 42)
SEQ ID NO: 22: base sequence into which mPing of polymorphism (22) has been inserted (marker 27)
SEQ ID NO: 23: nucleotide sequence in which mPing of polymorphism (23) is inserted (marker 199)
SEQ ID NO: 24: nucleotide sequence in which mPing of polymorphism (24) is inserted (marker 57)
SEQ ID NO: 25: base sequence in which mPing of polymorphism (25) is inserted (marker 234)
SEQ ID NO: 26: base sequence in which mPing of polymorphism (26) is inserted (marker 299)
SEQ ID NO: 27: base sequence in which mPing of polymorphism (27) is inserted (marker 304)
SEQ ID NO: 28: base sequence in which mPing of polymorphism (28) is inserted (marker 31)
SEQ ID NO: 29: base sequence in which mPing of polymorphism (29) is inserted (marker 203)
SEQ ID NO: 30: nucleotide sequence in which mPing of polymorphism (30) is inserted (marker 309)
SEQ ID NO: 31: base sequence in which mPing of polymorphism (31) is inserted (marker 317)
SEQ ID NO: 32: base sequence in which mPing of polymorphism (32) is inserted (marker 324)
SEQ ID NO: 33: base sequence in which mPing of polymorphism (33) is inserted (marker 102)
SEQ ID NO: 34: base sequence in which mPing of polymorphism (34) is inserted (marker 371)
SEQ ID NO: 35: base sequence in which mPing of polymorphism (35) is inserted (marker 348)
SEQ ID NO: 36: base sequence in which mPing of polymorphism (36) is inserted (marker 392)
SEQ ID NO: 37: base sequence in which mPing of polymorphism (37) is inserted (marker 394)
SEQ ID NO: 38: base sequence in which mPing of polymorphism (38) is inserted (marker 48)
SEQ ID NO: 39: base sequence in which mPing of polymorphism (39) is inserted (marker 411)
SEQ ID NO: 40: base sequence in which mPing of polymorphism (40) is inserted (marker 413)
SEQ ID NO: 41: base sequence in which mPing of polymorphism (41) is inserted (marker 208)
SEQ ID NO: 42: base sequence in which mPing of polymorphism (42) is inserted (marker 217)
SEQ ID NO: 43: base sequence in which mPing of polymorphism (43) is inserted (marker 417)
SEQ ID NO: 44: base sequence in which mPing of polymorphism (44) is inserted (marker 224)
SEQ ID NO: 45: base sequence in which mPing of polymorphism (45) is inserted (marker 420)
SEQ ID NO: 46: base sequence in which mPing of polymorphism (46) is inserted (marker 426)
SEQ ID NO: 47: Base sequence in which mPing of polymorphism (47) is inserted (marker 429)
SEQ ID NO: 48: base sequence in which mPing of polymorphism (48) is inserted (marker 436)
SEQ ID NO: 49: base sequence of primer 35PCR-F SEQ ID NO: 50: base sequence of primer 35PCR-R SEQ ID NO: 51: base sequence of primers 35P-F3 and 35N-F3 SEQ ID NO: 52: base sequence sequence number of primer 35P-B3 53: nucleotide sequences of primers 35P-FIP and 35N-FIP SEQ ID NO: 54: nucleotide sequences of primer 35P-BIP SEQ ID NO: 55: nucleotide sequences of primer 35P-LF SEQ ID NO: 56: nucleotide sequences of primer 35P-LB SEQ ID NO: 57: Primer 35N-B3 nucleotide sequence SEQ ID NO: 58: Primer 35N-BIP nucleotide sequence SEQ ID NO: 59: Primer 35N-LF nucleotide sequence SEQ ID NO: 60: Primer 35N-LB nucleotide sequence SEQ ID NO: 61: Polymorphism (1) Bases without mPing inserted Column (marker 35)
SEQ ID NO: 62: Nucleotide sequence in which mPing of polymorphism (2) is not inserted (Marker 39)
SEQ ID NO: 63: nucleotide sequence in which mPing of polymorphism (3) is not inserted (marker 26)
SEQ ID NO: 64: base sequence in which mPing of polymorphism (4) is not inserted (marker 52)
SEQ ID NO: 65: nucleotide sequence in which mPing of polymorphism (5) is not inserted (marker 186)
SEQ ID NO: 66: base sequence in which mPing of polymorphism (6) is not inserted (marker 233)
SEQ ID NO: 67: nucleotide sequence in which mPing of polymorphism (7) is not inserted (marker 4)
SEQ ID NO: 68: base sequence in which mPing of polymorphism (8) is not inserted (marker 189)
SEQ ID NO: 69: nucleotide sequence in which mPing of polymorphism (9) is not inserted (marker 239)
SEQ ID NO: 70: base sequence in which mPing of polymorphism (10) is not inserted (marker 240)
SEQ ID NO: 71: base sequence in which mPing of polymorphism (11) is not inserted (marker 241)
SEQ ID NO: 72: Nucleotide sequence in which mPing of polymorphism (12) is not inserted (marker 68)
SEQ ID NO: 73: Nucleotide sequence in which mPing of polymorphism (13) is not inserted (marker 245)
SEQ ID NO: 74: nucleotide sequence in which mPing of polymorphism (14) is not inserted (marker 253)
SEQ ID NO: 75: base sequence in which mPing of polymorphism (15) is not inserted (marker 191)
SEQ ID NO: 76: nucleotide sequence in which mPing of polymorphism (16) is not inserted (marker 261)
SEQ ID NO: 77: base sequence in which mPing of polymorphism (17) is not inserted (marker 77)
SEQ ID NO: 78: Base sequence in which mPing of polymorphism (18) is not inserted (marker 265)
SEQ ID NO: 79: Nucleotide sequence in which mPing of polymorphism (19) is not inserted (Marker 83)
SEQ ID NO: 80: Nucleotide sequence in which mPing of polymorphism (20) is not inserted (marker 244)
SEQ ID NO: 81: Nucleotide sequence in which mPing of polymorphism (21) is not inserted (marker 42)
SEQ ID NO: 82: Base sequence without marker of polymorphism (22) mPing (marker 27)
SEQ ID NO: 83: nucleotide sequence in which mPing of polymorphism (23) is not inserted (marker 199)
SEQ ID NO: 84: base sequence in which mPing of polymorphism (24) is not inserted (marker 57)
SEQ ID NO: 85: Base sequence without polymorphism (25) mPing inserted (marker 234)
SEQ ID NO: 86: nucleotide sequence in which mPing of polymorphism (26) is not inserted (marker 299)
SEQ ID NO: 87: nucleotide sequence in which mPing of polymorphism (27) is not inserted (marker 304)
SEQ ID NO: 88: nucleotide sequence in which mPing of polymorphism (28) is not inserted (marker 31)
SEQ ID NO: 89: nucleotide sequence in which mPing of polymorphism (29) is not inserted (marker 203)
SEQ ID NO: 90: Nucleotide sequence in which mPing of polymorphism (30) is not inserted (marker 309)
SEQ ID NO: 91: nucleotide sequence in which mPing of polymorphism (31) is not inserted (marker 317)
SEQ ID NO: 92: base sequence in which mPing of polymorphism (32) is not inserted (marker 324)
SEQ ID NO: 93: Nucleotide sequence in which mPing of polymorphism (33) is not inserted (marker 102)
SEQ ID NO: 94: base sequence in which mPing of polymorphism (34) is not inserted (marker 371)
SEQ ID NO: 95: nucleotide sequence in which mPing of polymorphism (35) is not inserted (marker 348)
SEQ ID NO: 96: base sequence in which mPing of polymorphism (36) is not inserted (marker 392)
SEQ ID NO: 97: nucleotide sequence in which mPing of polymorphism (37) is not inserted (marker 394)
SEQ ID NO: 98: Nucleotide sequence in which mPing of polymorphism (38) is not inserted (marker 48)
SEQ ID NO: 99: base sequence in which mPing of polymorphism (39) is not inserted (marker 401 or 401)
SEQ ID NO: 100: nucleotide sequence in which mPing of polymorphism (40) is not inserted (marker 413)
SEQ ID NO: 101: nucleotide sequence in which mPing of polymorphism (41) is not inserted (marker 208)
SEQ ID NO: 102: base sequence in which mPing of polymorphism (42) is not inserted (marker 217)
SEQ ID NO: 103: base sequence in which mPing of polymorphism (43) is not inserted (marker 417)
SEQ ID NO: 104: base sequence in which mPing of polymorphism (44) is not inserted (marker 224)
SEQ ID NO: 105: base sequence in which mPing of polymorphism (45) is not inserted (marker 420)
SEQ ID NO: 106: base sequence in which mPing of polymorphism (46) is not inserted (marker 426)
SEQ ID NO: 107: Nucleotide sequence in which mPing of polymorphism (47) is not inserted (marker 429)
SEQ ID NO: 108: base sequence in which mPing of polymorphism (48) is not inserted (marker 436)

Claims (7)

About one rice polymorphism selected from the group which consists of the following polymorphisms (1)-(48) about the rice genome contained in a sample, 1 type chosen from the group which consists of the following processes [1]-[41] A method for identifying a variety of gramineous plants, comprising performing and confirming the above steps.
[Polymorphism (1) to (48)]
Polymorphism (1): Presence / absence of mPing insertion site between nucleotide residues 701 and 702 of the nucleotide sequence of SEQ ID NO: 61 Polymorphism (2): 673 of nucleotide sequence of SEQ ID NO: 62 nucleotide residues and 674 th presence of mPing insertion site between nucleotide residues polymorphism (3): mPing insertion site between 639 th nucleotide residues and 640 th nucleotide residues of nucleotide sequence of SEQ ID NO: 63 Presence polymorphism (4): Presence polymorphism of the mPing insertion site between nucleotide residues 730 and 731 of the nucleotide sequence of SEQ ID NO: 64 (5): 692 of nucleotide sequence of SEQ ID NO: 65 nt residues and 693 of the presence or absence of mPing insertion site between nucleotide residues polymorphisms (6): SEQ 736 th nucleotide residues and 737 th presence of mPing insertion site between nucleotide residues polymorphism of the nucleotide sequence of issue 66 (7): SEQ ID NO: 67 of the 712 th nucleotide sequence nucleotide residues and 713 th whether polymorphisms mPing insertion site between nucleotide residues (8): 685 th nucleotide residues and 686 th presence of mPing insertion site between nucleotide residues polymorphism of the nucleotide sequences of SEQ ID NO: 68 (9) : Polymorphism of presence or absence of mPing insertion site between nucleotide residues 738 and 739 in the nucleotide sequence of SEQ ID NO: 69 (10): nucleotide residues 733 and 734 in the nucleotide sequence of SEQ ID NO: 70 th presence of mPing insertion site between nucleotide residues polymorphism (11): salts of SEQ ID NO: 71 713 th nucleotide residues and 714 th presence of mPing insertion site between nucleotide residues polymorphism sequence (12): SEQ ID NO: 72 of the 768 th nucleotide residues and 769 th nucleotide residues of nucleotide sequence whether mPing insertion site between polymorphism (13): 732 th nucleotide residues and 733 th presence of mPing insertion site between nucleotide residues polymorphism of the nucleotide sequences of SEQ ID NO: 73 (14): SEQ ID NO: 74 Polymorphism (15) of presence or absence of mPing insertion site between the 531st nucleotide residue and the 532nd nucleotide residue of the nucleotide sequence of SEQ ID NO: 75: the 722nd nucleotide residue and the 723nd nucleotide residue of the nucleotide sequence of SEQ ID NO: 75 Presence or absence polymorphism (16) of mPing insertion site between groups: of the nucleotide sequence of SEQ ID NO: 76 Polymorphism of presence or absence of mPing insertion site between nucleotide positions 753 and 754 (17): between nucleotide positions 727 and 728 of the nucleotide sequence of SEQ ID NO: 77 Polymorphism of presence / absence of mPing insertion site (18): Polymorphism of presence / absence of mPing insertion site between nucleotide residues 716 and 717 of the nucleotide sequence of SEQ ID NO: 78 (19): nucleotide of SEQ ID NO: 79 Presence / absence polymorphism of the mPing insertion site between the 632nd nucleotide residue and the 633rd nucleotide residue of the sequence (20): between the 730th nucleotide residue and the 731st nucleotide residue of the nucleotide sequence of SEQ ID NO: 80 whether mPing insertion site between polymorphism (21): the nucleotide sequence of SEQ ID NO: 81 686 Eye nucleotide residues and 687 th presence of mPing insertion site between nucleotide residues polymorphism (22): mPing between 727 th nucleotide residues and 728 th nucleotide residues of nucleotide sequence of SEQ ID NO: 82 Insertion site polymorphism (23): mPing insertion site polymorphism between nucleotide positions 803 and 804 of the nucleotide sequence of SEQ ID NO: 83 (24): nucleotide sequence of SEQ ID NO: 84 Polymorphism of presence or absence of mPing insertion site between the 694th nucleotide residue and the 695th nucleotide residue (25): between the 728th nucleotide residue and the 729th nucleotide residue of the nucleotide sequence of SEQ ID NO: 85 whether mPing insertion site at the polymorphic (26): the nucleotide sequence of SEQ ID NO: 86 656 th Kureochido residues and 657 th nucleotide residue mPing inserted existence of site polymorphisms between (27): mPing insertion site between 703 th nucleotide residues and 704 th nucleotide residues of nucleotide sequence of SEQ ID NO: 87 Presence polymorphism (28): Presence polymorphism of mPing insertion site between nucleotide residues 704 and 705 of the nucleotide sequence of SEQ ID NO: 88 (29): 696 nucleotide sequence of SEQ ID NO: 89 Polymorphism (30) of mPing insertion site between nucleotide number 6 and nucleotide number 697 (m): mPing between nucleotide number 800 and nucleotide number 801 of the nucleotide sequence of SEQ ID NO: 90 Insertion site polymorphism (31): Nucleotide 747 in the nucleotide sequence of SEQ ID NO: 91 Polymorphism (32) of presence or absence of mPing insertion site between tide residue and 748th nucleotide residue: mPing insertion site between nucleotide 722 and nucleotide 723 of nucleotide sequence of SEQ ID NO: 92 Presence / absence polymorphism (33): Presence polymorphism of the mPing insertion site between the 650th and 651th nucleotide residues of the nucleotide sequence of SEQ ID NO: 93 (34): 733 of the nucleotide sequence of SEQ ID NO: 94 Polymorphism (35) of presence or absence of mPing insertion site between nucleotide residues 734 and 734: mPing between nucleotide residues 578 and 579 of the nucleotide sequence of SEQ ID NO: 95 the presence or absence of the insertion site polymorphism (36): 879 th nucleotide residues of nucleotide sequence of SEQ ID NO: 96 When 880 th presence of mPing insertion site between nucleotide residues polymorphism (37): Non-mPing insertion site between 718 th nucleotide residues and 719 th nucleotide residues of nucleotide sequence of SEQ ID NO: 97 multi Type (38): Presence / absence of mPing insertion site between nucleotides 756 and 757 of the nucleotide sequence of SEQ ID NO: 98 Polymorphism (39): nucleotide 689 of the nucleotide sequence of SEQ ID NO: 99 residues and 69 0 th nucleotide residue mPing inserted existence of site polymorphisms between (40): mPing insertion site between 733 th nucleotide residues and 734 th nucleotide residues of nucleotide sequence of SEQ ID NO: 100 Polymorphism (41): nucleotide position 729 of the nucleotide sequence of SEQ ID NO: 101 730 th nucleotide residue mPing inserted existence of site polymorphisms between (42): Non polymorphism mPing insertion site between 705 th nucleotide residues and 706 th nucleotide residues of nucleotide sequence of SEQ ID NO: 102 (43): Presence / absence polymorphism of the mPing insertion site between the 603rd nucleotide residue and the 604th nucleotide residue of the nucleotide sequence of SEQ ID NO: 103 (44): 727th nucleotide residue of the nucleotide sequence of SEQ ID NO: 104 whether mPing insertion site between the group and the 728 th nucleotide residue polymorphisms (45): Non-mPing insertion site between 734 th nucleotide residues and 735 th nucleotide residues of nucleotide sequence of SEQ ID NO: 105 Polymorphism (46): nucleotide 653 of the nucleotide sequence of SEQ ID NO: 106 Whether mPing insertion site between the group and the 654 th nucleotide residue polymorphisms (47): Non-mPing insertion site between 726 th nucleotide residues and 727 th nucleotide residues of nucleotide sequence of SEQ ID NO: 107 Polymorphism (48): Presence / absence of mPing insertion site between nucleotide residues 626 and 627 of the nucleotide sequence of SEQ ID NO: 108 [steps [1] to [41]]
Step [1]: When mPing is inserted in the polymorphism (1), it is determined that the test subject is at a glance or tender. Step [2]: mPing is inserted in the polymorphism (2) If the mPing is not inserted in the polymorphism (6), (12), (25) or (31), (36) or (37), the test subject is determined to be Akitakomachi Step [3]: When mPing is inserted in polymorphism (3), and mPing is not inserted in polymorphism (6), (10) or (11), and (15), It is determined that the test subject is Hinohikari or Kuma no Mori [4]: When mPing is inserted in polymorphism (4) or (5), the test subject is Nanatsuboshi Judgment [5]: When mPing is inserted in the polymorphisms (2) and (6), it is determined that the test subject is an eclipse [6]: mPing in the polymorphisms (7) and (8) Step 7 to determine that the test subject is Kirara 397 when mPing is inserted [7]: mPing is inserted in polymorphism (9), and mPing is not inserted in polymorphism (43) In this case, the step [8] of determining that the subject to be examined is straight; when the mPing is inserted in the polymorphism (10) or (11) and the mPing is not inserted in the polymorphism (3). The step [9] of determining that the test subject is a fragile splash is determined when the mPing is inserted in the polymorphisms (2) and (12), and the test subject is determined to be a romantic. Step [10]: When mPing is inserted in the polymorphism (13), it is determined that the test subject is Kinuhikari [11]: When mPing is inserted in the polymorphism (14) In addition, it is determined that the test subject is a dream of Asahi [12]: When mPing is inserted in polymorphisms (8) and (15), it is determined that the test subject is dreamy. Step [13]: When mPing is inserted in the polymorphism (16), it is determined that the test object is fungus [14]: mPing in the polymorphism (17) or (18) Step 15 to determine that the test subject is a glossy princess when the mPing is inserted in the polymorphism (19). Determining step [16]: a step of determining that the test subject is a dream sushi when mPing is inserted in the polymorphism (20) and no mPing is inserted in the polymorphism (12) [ 17]: When the mPing is inserted in the polymorphism (21) and the mPing is not inserted in the polymorphism (19), it is determined that the test subject is a diaper or a hashimo [18]: When mPing is inserted in the polymorphism (22) or (23), determining that the test subject is fluffy [19]: polymorphism (7) , (15), ( 24), when mPing is inserted, it is determined that the subject to be tested is a dream [20]: mPing is inserted in polymorphism (25), and polymorphism (2) And (9) when mPing is not inserted, determining that the test subject is unsightly [21]: When mPing is inserted in polymorphism (26), the test subject is Step [22] for determining that it is a kinmusume step [23]: When mPing is inserted in the polymorphism (27), a step [23] for determining that the test subject is Aichi Kaori [28] ) Or (29), when mPing is inserted, determining that the test subject is rough [24]: When mPing is inserted in polymorphism (30), the test subject is Step [25] of determining that it is a dream Mizuho: Step [26] of determining that the subject to be tested is Sasanishiki when mPing is inserted in the polymorphism (31). When the mPing is inserted in the polymorphism (32), determining that the test subject is a milky queen [27]: When the mPing is inserted in the polymorphism (33) or (34) The step of determining that the test subject is a chickworm [28]: mPing is inserted in the polymorphism (35), and mPing is inserted in the polymorphism (12) or (38) and (39) or (40). In the case where no P is inserted, it is determined that the test subject is Himenomochi, Manamusume or Chiyonishiki [29]: When mPing is inserted in the polymorphism (36) or (37), the test is performed. target step [30] to determine that the rice Kogane is: polymorphisms (3) or (15), when the mPing is inserted in (38), A step of determining that the test object is a child of wind [31]: a step of determining that the test target is a cottonhead when mPing is inserted in the polymorphism (39) or (40) [32] : When mPing is inserted in the polymorphism (41) or (42), it is determined that the test subject is squirting [33]: mPing is inserted in the polymorphism (43) If the polymorphism (45) does not have mPing inserted, it is determined that the subject to be tested is a step [34]: when the polymorphism (44) has mPing inserted, Step [35] for determining that the test object is a kitemochi: When the mPing is inserted in the polymorphism (45), it is determined that the test target is a bare bran [36]: When mPing is inserted in the polymorphism (46) or (47), determining that the subject is Yamada Nishiki [37]: mPing is inserted in the polymorphism (48) Step [38]: When mPing is inserted in polymorphisms (35) and (38), the test subject is stuck. Determining that there is a step [39]: When mPing is inserted in polymorphism (3) and (6), (10) or (11), the test object is a Yukinko dance Determining step [40]: When mPing is inserted in polymorphisms (12) and (20), determinating that the test subject is a light beam [41]: mPing in polymorphism (12) Is inserted, polymorphism (2), (1 3), (20), (26), and (35) or (38), if mPing is not inserted, determine that the test subject is clear The variety identification method according to claim 1, wherein any one of the polymorphisms (1) to (48) is as follows.
Polymorphism (1): Presence / absence of mPing insertion site , which is nucleotide residues 702 to 1131 of the nucleotide sequence of SEQ ID NO: 1 Polymorphism (2): nucleotide residues 674 to 1103 of the nucleotide sequence of SEQ ID NO: 2 Presence / absence polymorphism of a certain mPing insertion site (3): Presence / absence polymorphism of mPing insertion site that is the 640th to 1069th nucleotide residues of the base sequence of SEQ ID NO: 3 (4): 731 to 1160 of the base sequence of SEQ ID NO: 4 th presence of mPing insertion site is a nucleotide residue polymorphism (5): SEQ ID NO: 5 whether mPing insertion site is 693-1122 nt residues of nucleotide sequence polymorphisms (6): SEQ ID NO: 6 whether mPing insertion site is 737-1166 nt residues of nucleotide sequence polymorphisms (7): 713-1142 th of the base sequence of SEQ ID NO: 7 Whether mPing insertion site is a nucleotide residue polymorphisms (8): Non polymorphism mPing insertion site is 686-1115 nt residues of the nucleotide sequence of SEQ ID NO: 8 (9): nucleotide sequence of SEQ ID NO: 9 MPing insertion site polymorphism (10) which is the 739 to 1168th nucleotide residue of (10): Polymorphism polymorphism (11) of the mPing insertion site which is the 734 to 1163rd nucleotide residue of the nucleotide sequence of SEQ ID NO: 10: Presence / absence of mPing insertion site that is the 714 to 1143th nucleotide residue of the nucleotide sequence of SEQ ID NO: 11 (12): Presence or absence of the mPing insertion site that is the 769 to 1198th nucleotide residue of the nucleotide sequence of SEQ ID NO: 12 polymorphism (13): mPing insert is from 733 to 1162 th nucleotide residues of nucleotide sequence of SEQ ID NO: 13 Position of whether polymorphisms (14): Non polymorphism mPing insertion site is 532-961 nt residues of the nucleotide sequence of SEQ ID NO: 14 (15): 723-1152 nt of the nucleotide sequence of SEQ ID NO: 15 whether mPing insertion site is a residue polymorphism (16): Non polymorphism mPing insertion site is 754-1183 nt residues of the nucleotide sequence of SEQ ID NO: 16 (17): the nucleotide sequence of SEQ ID NO: 17 Presence / absence polymorphism of mPing insertion site which is the 728 to 1157th nucleotide residue (18): Presence polymorphism of the mPing insertion site which is the 717 to 1146th nucleotide residue of the nucleotide sequence of SEQ ID NO: 18 (19): Sequence Presence / absence polymorphism of mPing insertion site which is the nucleotide residues 633 to 1062 in the nucleotide sequence of No. 19 (20): SEQ ID NO: 2 Presence / absence polymorphism of the mPing insertion site which is the 731st to 1160th nucleotide residue of the base sequence of 0 (21): Presence / absence polymorphism of the mPing insertion site which is the 687th to 1116th nucleotide residue of the base sequence of SEQ ID NO: 21 (22): presence or absence polymorphism of mPing insertion site which is 728 to 1157th nucleotide residue of base sequence of SEQ ID NO: 22 (23): mPing which is 804 to 1233th nucleotide residue of base sequence of SEQ ID NO: 23 Insertion site polymorphism (24): mPing insertion site polymorphism (25): nucleotide sequence from 729 to 1158 of the nucleotide sequence of SEQ ID NO: 25 whether mPing insertion site is a nucleotide residue polymorphisms (26): the nucleotide sequence of SEQ ID NO: 26 657-1086 Whether mPing insertion site is the eye of nucleotide residues polymorphism (27): Non polymorphism mPing insertion site is 704-1133 nt residues of the nucleotide sequence of SEQ ID NO: 27 (28): SEQ ID NO: 28 whether mPing insertion site is 705-1134 nt residues of nucleotide sequence polymorphisms (29): Non polymorphism mPing insertion site is 697-1126 nt residues of the nucleotide sequence of SEQ ID NO: 29 (30 ): Presence / absence polymorphism of the mPing insertion site that is the 801 to 1230th nucleotide residue of the base sequence of SEQ ID NO: 30 (31): mPing insertion site that is the 748 to 1177th nucleotide residue of the base sequence of SEQ ID NO: 31 Presence polymorphism (32): m which is nucleotide residues 723 to 1152 of the nucleotide sequence of SEQ ID NO: 32 whether ing insertion site polymorphism (33): Non polymorphism mPing insertion site is 651-1080 nt residues of the nucleotide sequence of SEQ ID NO: 33 (34): 734-1163 th of the base sequence of SEQ ID NO: 34 the presence or absence of mPing insertion site is a nucleotide residue polymorphisms (35): Non polymorphism mPing insertion site is 579-1008 nt residues of the nucleotide sequence of SEQ ID NO: 35 (36): SEQ ID NO: 36 bases Presence / absence polymorphism of mPing insertion site which is nucleotides 880 to 1309 of the sequence (37): Presence / absence polymorphism of mPing insertion site which is nucleotides 732 to 1161 of the nucleotide sequence of SEQ ID NO: 37 (38) : Presence or absence polymorphism of the mPing insertion site which is the nucleotide residue of the 757 to 1186th nucleotide of the nucleotide sequence of SEQ ID NO: 38 (39 ): Presence / absence polymorphism of the mPing insertion site which is the 690 to 1119th nucleotide residue of the base sequence of SEQ ID NO: 39 (40): mPing insertion site which is the 734th to 1163rd nucleotide residue of the base sequence of SEQ ID NO: 40 Presence polymorphism (41): Presence polymorphism of mPing insertion site which is the 730th to 1159th nucleotide residues of the nucleotide sequence of SEQ ID NO: 41 (42): 706 to 1135th nucleotide residue of the nucleotide sequence of SEQ ID NO: 42 whether mPing insertion site is a group polymorphism (43): Non polymorphism mPing insertion site is 604-1033 nt residues of the nucleotide sequence of SEQ ID NO: 43 (44): the nucleotide sequence of SEQ ID NO: 44 728 ～1157 th presence of mPing insertion site is a nucleotide residue polymorphisms (45): the nucleotide sequence of SEQ ID NO: 45 7 5-1164 th presence of mPing insertion site is a nucleotide residue polymorphisms (46): Non-mPing insertion site is 654-1083 nt residues of the nucleotide sequence of SEQ ID NO: 46 polymorphisms (47): sequence Presence / absence of mPing insertion site which is the 727 to 1156th nucleotide residue of the nucleotide sequence of No. 47 (48): Presence or absence of the mPing insertion site which is the 627th to 1056th nucleotide residue of the nucleotide sequence of SEQ ID No. 48 The breed identification method according to claim 1 or 2 , wherein a primer set composed of two or more primers capable of detecting one or more polymorphisms selected from the group consisting of polymorphisms (1) to (48) is used . The method for identifying a variety according to claim 3, wherein the primer set is a primer set for PCR or a primer set for LAMP. The primer set is a primer consisting of the base sequence of SEQ ID NO: 51, a primer consisting of the base sequence of SEQ ID NO: 52, a primer consisting of the base sequence of SEQ ID NO: 53, a primer consisting of the base sequence of SEQ ID NO: 54, and a base sequence of SEQ ID NO: 55 And / or a primer set consisting of a primer consisting of the base sequence of SEQ ID NO: 56, and / or a primer consisting of the base sequence of SEQ ID NO: 51, a primer consisting of the base sequence of SEQ ID NO: 57, and a base of SEQ ID NO: 53 The breed identification method according to claim 4, comprising a primer comprising the sequence, a primer comprising the base sequence of SEQ ID NO: 58, a primer comprising the base sequence of SEQ ID NO: 59, and a primer comprising the base sequence of SEQ ID NO: 60 . The breed identification method according to claim 1 or 2, wherein one or more probes capable of detecting one or more polymorphisms selected from the group consisting of polymorphisms (1) to (48) are used . A primer set composed of two or more primers capable of detecting one or more polymorphisms selected from the group consisting of polymorphisms (1) to (48), and a group consisting of polymorphisms (1) to (48) The method according to claim 1 or 2, wherein a detection kit comprising at least one selected from the group consisting of probes capable of detecting at least one selected polymorphism is used .